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| 1 | Activation of JAK-STAT pathway is required for platelet-derived growth factor-induced proliferation of pancreatic stellate cells显示文摘AIM: To clarify the role of Janus kinase-signal transducersand activators of transcription (JAK-STAT) pathway in platelet-derived growth factor (PDGF) induced proliferation in activated pancreatic stellate cells (PSCs).METHODS: PSCs were isolated from rat pancreas tissue, and used in their culture-activated, myofibroblast-like phenotype. STAT-specific binding activity was assessed by electrophoretic mobility shift assay. Activation of Src, JAK2,STAT1, STAT3, and ERK was determined by Western blotting using anti-phosphospecific antibodies. Cell proliferation was assessed by measuring the incorporation of 5-bromo-2′deoxyuridine.RESULTS: PDGF-BB induced STAT-specific binding activity, and activation of Src, JAK2, STAT1, STAT3, and ERK. Ethanol and acetaldehyde at clinically relevant concentrations decreased basal activation of JAK2 and STAT3. PDGFinduced activation of STAT1 and STAT3 was inhibited bya Src inhibitor PP1 and a JAK2 inhibitor AG490, whereasPDGF-induced activation of ERK was inhibited by PP1, and not by AG490. PDGF-induced proliferation was inhibited by PP1 and AG490 as well as by STAT3 antisense oligonucleotide.CONCLUSION: PDGF-BB activated JAK2-STAT pathwayvia Src-dependent mechanism. Activation of JAK2-STAT3pathway, in addition to ERK, may play a role in PDGF-induced proliferation of PSCs. | Atsushi Masamune Masahiro Satoh Kazuhiro Kikuta Noriaki Suzuki Tooru Shimosegawa | 2005 | World Journal of Gastroenterology2005，11，22： | 15 |
| 2 | Endothelin-1 stimulates contraction and migration of rat pancreatic stellate cells显示文摘AIM: To examine the ability of ET-1 to affect the cell functions of PSCs and the underlying molecular mechanisms.METHODS: PSCs were isolated from the pancreas of male Wistar rats after perfusion with collagenase, and cells between passages two and five were used. Expression of ET-1 and ET receptors was assessed by reverse transcription-PCR and immunostaining. Phosphorylation of myosin regulatory light chain (MLC), extracellular-signal regulated kinase (ERK), and Akt was examined by Western blotting. Contraction of PSCs was assessed on hydrated collagen lattices. Cell migration was examined using modified Boyden chambers. Cell proliferation was assessed by measuring the incorporation of 5-bromo-2'-deoxyuridine.RESULTS: Culture-activated PSCs expressed ETA and ETB receptors, and ET-1. ET-1 induced phosphorylation of MLC and ERK, but not Akt. ET-1 induced contraction and migration,but did not alter proliferation of PSCs. ET-1-induced contraction was inhibited by an ETA receptor antagonist BQ-123 and an ETB receptor antagonist BQ-788, whereas migration was inhibited by BQ-788 but not by BQ-123. A Rho kinase inhibitor Y-27632 abolished both contraction and migration.CONCLUSION: ET-1 induced contraction and migration of PSCs through ET receptors and activation of Rho-Rho kinase. ETA and ETB receptors play different roles in the regulation of these cellular functions in response to ET-1. | Atsushi Masamune Masahiro Satoh Kazuhiro Kikuta Noriaki Suzuki Kennichi Satoh Tooru Shimosegawa | 2005 | World Journal of Gastroenterology2005，11，39： | 11 |
| 3 | Establishment and characterization of a rat pancreatic stellate cell line by spontaneous immortalization显示文摘AIM: Activated pancreatic stellate cells (PSCs) have been implicated in the pathogenesis of pancreatic fibrosis and inflammation. Primary PSCs can be subcultured only several times because of their limited growth potential. A continuous cell line may therefore be valuable in studying molecular mechanisms of these pancreatic disorders. The aim of this study was to establish a cell line of rat PSCs by spontaneous immortalization.METHODS: PSCs were isolated from the pancreas of male Wistar rats, and conventional subcultivation was performed repeatedly. Telomerase activity was measured using the telomere repeat amplification protocol. Activation of transcription factors was assessed by electrophoretic mobility shift assay.Activation of mitogen-activated protein (MAP) kinases was examined by Western blotting using anti-phosphospecific antibodies. Expression of cytokine-induced neutrophil chemoattractant-1 was determined by enzyme immunoassay.RESULTS: Conventional subcultivation yielded actively growing cells. One clone was obtained after limiting dilution,and designated as SIPS. This cell line has been passaged repeatedly more than 2 years, and is thus likely immortalized.SIPS cells retained morphological characteristics of primary,culture-activated PSCs. SIPS expressed α-smooth muscle actin, glial acidic fibrillary protein, vimentin, desmin, type Ⅰ collagen, fibronectin, and prolyl hydroxylases. Telomerase activity and p53 expression were negative. Proliferation of SIPS cells was serum-dependent, and stimulated with platelet-derived growth factor-BB through the activation of extracellular signal-regulated kinase. Interleukin-1β activated nuclear factor-κB, activator protein-1, and MAP kinases.Interleukin-1β induced cytokine-induced neutrophil chemoattractant-1 expression through the activation of nuclear factor-κB and MAP kinases.CONCLUSION: SIPS cells can be useful for in vitro studies of cell biology and signal transduction of PSCs. | Atsushi Masamune Masahiro Satoh Kazuhiro Kikuta Noriaki Suzuki Tooru Shimosegawa | 2003 | World Journal of Gastroenterology2003，9，12： | 11 |
| 4 | Green tea polyphenol epigallocatechin-3-gallate blocks PDGF-induced proliferation and migration of rat pancreatic stellate cells显示文摘AIM: To clarify the effects of epigallocatechin-3-gallate (EGCG) on the platelet-derived growth factor (PDGF)-BBinduced proliferation and migration of pancreatic stellate cells (PSCs).METHODS: PSCs were isolated from rat pancreas tissue and used in their culture-activated, myofibroblast-like phenotype. Cell proliferation was assessed by measuring the incorporation of 5-bromo-2'-deoxyuridine. Cell migration was assessed using modified Boyden chambers. CyclinD1, p21waf1, and p27kip1 expression and phosphorylation of PDGF β-receptor, extracellular signal-regulated kinase, and Akt were examined by Western blotting. Activation of phosphatidylinositol 3-kinase was examined by kinase assay using phosphatidylinositol as a substrate. Cell cycle was assessed by flow cytometry after staining with propidium iodide. RESULTS: EGCG at non-cytotoxic concentrations inhibited PDGF-induced proliferation and migration. This effect was associated with the inhibition of cell cycle progression beyondthe G1 phase, decreased cyclin D1 and increased p27Kip1 expression. EGCG inhibited tyrosine phosphorylation of PDGF β-receptor and downstream activation of extracellular signal-regulated kinase and phosphatidylinositol 3-kinase/ Akt pathways.CONCLUSION: EGCG inhibited PDGF-BB-induced proliferation and migration of PSCs through the inhibrdon of PDGF-mediated signaling pathways. | Atsushi Masamune Kazuhiro Kikuta Masahiro Satoh Noriaki Suzuki Tooru Shimosegawa | 2005 | World Journal of Gastroenterology2005，11，22： | 8 |
| 5 | Time to re-evaluate effects of renin-angiotensin system inhibitors on renal and cardiovascular outcomes in diabetic nephropathy显示文摘The use of renin-angiotensin system(RAS) inhibitors, such angiotensin converting enzyme inhibitors/angiotensin-Ⅱreceptor blockers, to slow progression of chronic kidney disease(CKD) in a large group dominated by elderly people in the real world is not supported by available evidence. Large-scale clinical trials had many faults,among them a lack of focus on the elderly. However,it would be difficult to conduct clinical trials of a similar scale in elderly CKD patients. Besides, progression ofkidney disease is often slow in elderly persons, and the vast majority of older adults with CKD will die before reaching end stage renal disease. Moreover, since it is not clear that progression of kidney disease, and even of proteinuric diabetic nephropathy, is not inhibited through the use of RAS inhibitors, the most patientcentric goal of therapy for many elderly individuals should be individualized. | Hiromichi Suzuki Tomohiro Kikuta Tsutomu Inoue Ukihiro Hamada | 2015 | World Journal of Nephrology2015，4，1： | 6 |
| 6 | Impaired glucose tolerance in acute pancreatitis显示文摘Acute pancreatitis(AP) is an acute inflammatory disease of the exocrine pancreas. In spite of the pivotal role of the endocrine pancreas in glucose metabolism, the impact of impaired glucose tolerance on AP has not been fully elucidated. A meta-analysis of seven observational studies showed that type 2 diabetes mellitus(DM) was associated with an increased risk of AP. The increased risk of AP shown in the meta-analysis was independent of hyperlipidemia, alcohol use and gallstones. Antidiabetic drugs including incretins might increase the risk of AP, but no intervention trials have confirmed this. Although a controversial finding, DM seems to be associated with severe attacks and organ failure in AP. We analyzed the results of a nationwide epidemiological survey of AP in Japan. We studied the impact of pre-existing DM on the clinical course of AP in 1954 cases for which information on DM status was available at the onset of AP. The prevalence of DM in AP patients(12.8%) was higher than that in the general population in Japan(10.5%). AP patients with DM had higher morbidity of cardiovascular and renal failure than those without DM. About 35% of the idiopathic AP patients with DM had renal failure. The mortality of AP patients with DM(4.0%) was higher than that of AP patients without DM(1.7%). If stratified by etiology, idiopathic, but not alcoholic or biliary, AP patients with DM were predisposed to increased mortality(9.7%). In conclusion, impaired glucose tolerance might have an impact on the development and clinical outcome of AP. However, the impact might depend on the cause of hyperglycemia, the condition of DM including severity, duration and treatment, and the characteristics of the AP patients including age, etiology and comorbidity. | Kazuhiro Kikuta Atsushi Masamune Tooru Shimosegawa | 2015 | World Journal of Gastroenterology2015，21，24： | 6 |
| 7 | IgG4-unrelated type 1 autoimmune pancreatitis显示文摘A 50-year-old male was referred to our hospital for the evaluation of hyperproteinemia.Fluorodeoxyglucose positron emission tomography revealed high fluorodeoxyglucose uptake in the pancreas,bilateral lacrimal glands,submandibular glands,parotid glands,bilateral pulmonary hilar lymph nodes,and kidneys.Laboratory data showed an elevation of hepatobiliary enzymes,renal dysfunction,and remarkably high immunoglobulin(Ig) G levels,without elevated serum IgG4.Abdominal computed tomography revealed swelling of the pancreatic head and bilateral kidneys.Endoscopic retrograde cholangiopancreatography showed an irregular narrowing of the main pancreatic duct in the pancreatic head and stricture of the lower common bile duct.Histological examination by endoscopic ultrasonography-guided fine-needle aspiration revealed findings of lymphoplasmacytic sclerosing pancreatitis without IgG4-positive plasma cells.Abnormal laboratory values and the swelling of several organs were improved by the treatment with steroids.The patient was diagnosed as having type 1 autoimmune pancreatitis(AIP) based on the International Consensus Diagnostic Criteria.Therefore,we encountered a case of compatible type 1 AIP without elevated levels of serum IgG4 or IgG4-positive plasma cells.This case suggests that AIP phenotypes are not always associated with IgG4. | Eriko Nakano Atsushi Kanno Atsushi Masamune Naoki Yoshida Seiji Hongo Shin Miura Tetsuya Takikawa Shin Hamada Kiyoshi Kume Kazuhiro Kikuta Morihisa Hirota Keisuke Nakayama Fumiyoshi Fujishima Tooru Shimosegawa | 2015 | World Journal of Gastroenterology2015，21，33： | 3 |
| 8 | Pancreatic duct drainage using EUS-guided rendezvous technique for stenotic pancreaticojejunostomy显示文摘The patient was a 30-year-old female who had undergone excision of the extrahepatic bile duct and Rouxen-Y hepaticojejunostomy for congenital biliary dilatation at the age of 7.Thereafter,she suffered from recurrent acute pancreatitis due to pancreaticobiliary maljunction and received subtotal stomach-preserving pancreaticoduodenectomy.She developed a pancreatic fistula and an intra-abdominal abscess after the operation.These complications were improved by percutaneous abscess drainage and antibiotic therapy.How ever,upper abdominal discomfort and the elevation of serum pancreatic enzymes persisted due to stenosis from the pancreaticojejunostomy.Because we could not accomplish dilation of the stenosis by endoscopic retrograde cholangiopancreatography,we tried an endoscopic ultrasonography(EUS) guided rendezvous technique for pancreatic duct drainage.After transgastric puncture of the pancreatic duct using an EUS-fine needle aspiration needle,the guidewire was inserted into the pancreatic duct and finally reached to the jejunum through the stenotic anastomosis.We changed the echoendoscope to an oblique-viewing endoscope,then grasped the guidewire and withdrew it through the scope.The stenosis of the pancreaticojejunostomy was dilated up to 4 mm,and a pancreatic stent was put in place.Though the pancreatic stent was removed after three months,the patient remained symptomfree.Pancreatic duct drainage using an EUS-guided rendezvous technique was useful for the treatment of a stenotic pancreaticojejunostomy after pancreaticoduodenectomy. | Tetsuya Takikawa Atsushi Kanno Atsushi Masamune Shin Hamada Eriko Nakano Shin Miura Hiroyuki Ariga Jun Unno Kiyoshi Kume Kazuhiro Kikuta Morihisa Hirota Hiroshi Yoshida Yu Katayose Michiaki Unno Tooru Shimosegawa | 2013 | World Journal of Gastroenterology2013，19，31： | 3 |
| 9 | Characterization of termetallic Pt-Ir-Au catalysts for NO decomposition显示文摘A termetallic catalyst of Pt-Ir-Au/Al2O3 for NOx decomposition was prepared by loading the metallic colloids in C2H5OH-H2O solution and a surfactant of polyvinyl pyrrolidone.Compared with an impregnated Pt/Al2O3 catalyst,the termetallic catalyst of PtIrAu811/Al2O3,with a Pt:Ir:Au atomic ratio of 8:1:1,exhibited higher NO decomposition and selectivity to N2.Transmission electron microscopy and X-ray diffraction were conducted to clarify the state of the supported metals and indicate three precious metals alloyed on the catalyst.In the study of NO-temperature programmed desorption,oxygen desorption on the PtIrAu811 catalyst shifted to the low temperature side compared to that on Pt/Al2O3,which correlated well with its higher catalytic performance in NO decomposition. | Akira Morikawa Kohei Okumura Masaru Ishii Koichi Kikuta Akihiko Suda Hirofumi Shinjo | 2011 | Rare Metals2011，30，1： | 3 |
| 10 | Diagnosis of autoimmune pancreatitis by EUS-FNA by using a 22-gauge needle based on the International Consensus Diagnostic Criteria显示文摘 | Atsushi Kanno Kazuyuki Ishida Shin Hamada Fumiyoshi Fujishima Jun Unno Kiyoshi Kume Kazuhiro Kikuta Morihisa Hirota Atsushi Masamune Kennichi Satoh Kenji Notohara Tooru Shimosegawa | 2012 | Gastrointestinal Endoscopy2012，，3： | 3 |
| 11 | miR‐197 induces epithelial–mesenchymal transition in pancreatic cancer cells by targeting p120 catenin显示文摘 | Shin Hamada Kennichi Satoh Shin Miura Morihisa Hirota Atsushi Kanno Atsushi Masamune Kazuhiro Kikuta Kiyoshi Kume Jun Unno Shinichi Egawa Fuyuhiko Motoi Michiaki Unno Tooru Shimosegawa | 2013 | J Cell Physiol2013，，6： | 3 |
| 12 | The angiotensin II type I receptor blocker olmesartan inhibits the growth of pancreatic cancer by targeting stellate cell activities in mice显示文摘 | Atsushi Masamune Shin Hamada Kazuhiro Kikuta Tetsuya Takikawa Shin Miura Eriko Nakano Tooru Shimosegawa | 2013 | Scandinavian Journal of Gastroenterology2013，，5： | 2 |
| 13 | Lipase production in two-step fed-batch culture of organic solvent-tolerant Pseudomonas aeruginosa LST-03显示文摘 | Ito T Kikuta H Nagamori E | 2001 | Journal of Biosciences Bioengineering2001，91，： | 1 |
| 14 | A pilot study on effectiveness of music therapy in hospice in Japan显示文摘 | Nakayama H Kikuta F Takeda H | | 0，，02： | 1 |
| 15 | 查看详情显示文摘 | Murikawa A Kikuta K Suda A | | 0，，： | 1 |
| 16 | Reorientation of cortional microtubales in the sub-apical region during tuberization in single-node stem segment of potato in culture显示文摘 | FUJINO K KODA Y KIKUTA Y | 1995 | Plant Cell Physiol1995，36，： | 1 |
| 17 | Milrinone for the treatment of cerebral vasospasm after subarachnoid hemorrhage:report of seven cases显示文摘 | Arakawa Y Kikuta K Hojo M | 2001 | Neurosurgery2001，48，4： | 1 |
| 18 | Prostaglandin and leukotriene ω-hydroxylases显示文摘 | Kikuta Y Kusunose E Kusunose M | 2002 | Prostaglandins Other Lipid Mediat2002，6869，： | 1 |
| 19 | Diffusion tensor fiber tractography for arteriovenous malformations: quantitative analyses to evaluate the corticospinal tract and optic radiation显示文摘 | Okada T Miki Y Kikuta K | 2007 | AJNR Am J Neuroradiol2007，28，6： | 1 |
| 20 | Effect of early optic canal unroofing on the outcome of visual functions in surgery for meningiomas of the tuberculum sellae and planum sphenoidale 显示文摘 | Nozaki K Kikuta K Takagi Y et d | 2008 | Neurosurgery2008，62，4： | 1 |
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