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| 1 | Lithium, an anti-psychotic drug, greatly enhances the generation of induced pluripotent stem cells显示文摘体的房间能由定义因素是进导致的 pluripotent 干细胞(iPSCs ) 的 reprogrammed。reprogramming 的低效率和 oncogenes 和病毒的向量的 genomic 集成限制了 iPSCs 的潜在的申请。这里,我们报导那锂(李) ,药过去常对待心情混乱,极大地从老鼠提高 iPSC 产生胚胎的成纤维细胞和人的脐的静脉 endothelial 房间。李与(Oct4 ) 或二个因素(OS 或好) 便于 iPSC 产生。支持 reprogramming 上的李的效果仅仅部分取决于它的主要目标 GSK3β。不同于另外的 GSK3β禁止者,李不仅增加 Nanog 的表示,而且提高 Nanog 的 transcriptional 活动。我们也发现李由经由 LSD1 的 downregulation 支持 epigenetic 修正施加它的效果, H3K4 特定的 histone demethylase。将 LSD1 击倒部分在提高 reprogramming 模仿李的效果。我们的结果不仅提供一个直接方法改进 iPSC 产生效率,而且为体的房间 reprogramming 作为一个批评调节的人识别了 histone demethylase。 | Quan Wang Xinxiu Xu Jun Li Jing Liu Haifeng Gu Ru Zhang Jiekai Chen Yin Kuang Jian Fei Cong Jiang Ping Wang Duanqing Pei Sheng Ding Xin Xie | 2011 | Cell Research2011，21，10： | 23 |
| 2 | Single-cell transcriptomics reveals regulators underlying immune cell diversity and immune subtypes associated with prognosis in nasopharyngeal carcinoma显示文摘Nasopharyngeal carcinoma(NPC)is an aggressive malignancy with extremely skewed ethnic and geographic distributions.Increasing evidence indicates that targeting the tumor microenvironment(TME)represents a promising therapeutic approach in NPC,highlighting an urgent need to deepen the understanding of the complex NPC TME.Here,we generated single-cell transcriptome profiles for 7581 malignant cells and 40,285 immune cells from fifteen primary NPC tumors and one normal sample.We revealed malignant signatures capturing intratumoral transcriptional heterogeneity and predicting aggressiveness of malignant cells.Diverse immune cell subtypes were identified,including novel subtypes such as CLEC9A^+dendritic cells(DCs).We further revealed transcriptional regulators underlying immune cell diversity,and cell–cell interaction analyses highlighted promising immunotherapeutic targets in NPC.Moreover,we established the immune subtype-specific signatures,and demonstrated that the signatures of macrophages,plasmacytoid dendritic cells(pDCs),CLEC9A^+DCs,natural killer(NK)cells,and plasma cells were significantly associated with improved survival outcomes in NPC.Taken together,our findings represent a unique resource providing in-depth insights into the cellular heterogeneity of NPC TME and highlight potential biomarkers for anticancer treatment and risk stratification,laying a new foundation for precision therapies in NPC. | Yu-Pei Chen Jian-Hua Yin Wen-Fei Li Han-Jie Li Dong-Ping Chen Cui-Juan Zhang Jia-Wei Lv Ya-Qin Wang Xiao-Min Li Jun-Yan Li Pan-Pan Zhang Ying-Qin Li Qing-Mei He Xiao-Jing Yang Yuan Lei Ling-Long Tang Guan-Qun Zhou Yan-Ping Mao Chen Wei Ke-Xu Xiong Hong-Bo Zhang Shi-Da Zhu Yong Hou Ying Sun Michael Dean Ido Amit Kui Wu Dong-Ming Kuang Gui-Bo Li Na Liu Jun Ma | 2020 | Cell Research2020，30，11： | 19 |
| 3 | Prognosis-related classifi cation and dynamic monitoring of immune status in patients with sepsis:A prospective observational study显示文摘BACKGROUND:The dynamic monitoring of immune status is crucial to the precise and individualized treatment of sepsis.In this study,we aim to introduce a model to describe and monitor the immune status of sepsis and to explore its prognostic value.METHODS:A prospective observational study was carried out in Zhongshan Hospital,Fudan University,enrolling septic patients admitted between July 2016 and December 2018.Blood samples were collected at days 1 and 3.Serum cytokine levels(e.g.,tumor necrosis factor-α[TNF-α],interleukin-10[IL-10])and CD14+monocyte human leukocyte antigen-D-related(HLA-DR)expression were measured to serve as immune markers.Classifi cation of each immune status,namely systemic inflammatory response syndrome(SIRS),compensatory anti-inflammatory response syndrome(CARS),and mixed antagonistic response syndrome(MARS),was defined based on levels of immune markers.Changes of immune status were classifi ed into four groups which were stabilization(SB),deterioration(DT),remission(RM),and non-remission(NR).RESULTS:A total of 174 septic patients were enrolled including 50 non-survivors.Multivariate analysis discovered that IL-10 and HLA-DR expression levels at day 3 were independent prognostic factors.Patients with MARS had the highest mortality rate.Immune status of 46.1%patients changed from day 1 to day 3.Among four groups of immune status changes,DT had the highest mortality rate,followed by NR,RM,and SB with mortality rates of 64.7%,42.9%,and 11.2%,respectively.CONCLUSIONS:Severe immune disorder defi ned as MARS or deterioration of immune status defi ned as DT lead to the worst outcomes.The preliminary model of the classifi cation and dynamic monitoring of immune status based on immune markers has prognostic values and is worthy of further investigation. | Jun Yin Yao Chen Jun-ling Huang Lei Yan Zhong-shu Kuang Ming-ming Xue Si Sun Hao Xiang Yan-yan Hu Zhi-min Dong Chao-yang Tong Chun-xue Bai Zhen-ju Song | 2021 | World Journal of Emergency Medicine2021，12，3： | 16 |
| 4 | First report of the South American tomato leafminer, Tuta absoluta (Meyrick), in China显示文摘Tuta absoluta(Meyrick) originated in South America and is one of the most serious pests of tomatoes. It is also known to attack other solanaceous crops, including potato, eggplant, pepper, tobacco, and weedy species such as black nightshade. After accidental introduction into Spain in 2006, this pest spread rapidly throughout Afro-Eurasia and has become a major threat to tomato production worldwide. Here, we report the first record of T. absoluta as an invasive pest in China. It was found in tomato fields in Ili Kazakg Autonomous Prefecture, Xinjiang Uygur Autonomous Region(Ili, Xinjiang), China, and its occurrence was confirmed by both morphological and molecular approaches. In Ili, T. absoluta has been found to attack eggplant, potato, and black nightshade. We found the larvae generally mining and feeding on leaves and boring into tomato fruits, with multiple larvae sometimes observed in a single fruit. Its infestation levels differ among the tomato fields and host species. In all of the surveyed tomato fields, T. absoluta infested 100% of plants. In some of the fields, up to 90% of the eggplant and 100% of the potato plants were infested. Since no natural enemies were found under field conditions, suitable management practices are urgently needed to stop the further spread of this destructive pest in China. | ZHANG Gui-fen MA De-ying WANG Yu-sheng GAO You-hua LIU Wan-xue ZHANG Rong FU Wen-jun XIAN Xiao-qing WANG Jun KUANG Meng WAN Fang-hao | 2020 | Journal of Integrative Agriculture2020，19，7： | 12 |
| 5 | TNF-α and IL-1RA Polymorphisms and Silicosis Susceptibility in Chinese Workers Exposed to Silica Particles:A Case-Control Study显示文摘Objective To assess the association of TNF- α and IL-1RA SNPs with the risk of silicosis in Chinese workers exposed to silica particles. Methods Case-control study design was used to enroll 68 silicotic patients induced by silica particles and 68 healthy workers matched for length of silica particle exposure as controls. Both cases and controls were from the same company in southwest China, and each of them was requested to complete a questionnaire. Blood samples were drawn for genomic DNA extraction from each participant. The genotyping of TNF-α (-238 and -308) and IL-1RA (+2018) was performed using polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) and SYBR green-based quantitative polymerase chain reaction (qPCR), respectively. Unconditional logistic regression model was used to estimate odds ratios (ORs) and their 95% confidential intervals (CI) for SNPs. Results No significant differences were found between cases and controls in particles exposure length, body mass index (BMI), and status of smoking and alcohol consumption except for age (P=0.001) and blood type (P=0.042). The frequencies of TNF-α (-238) and IL-1RA (+2018) genotypes in cases were significantly different from those in controls, (P=0.001 and P=0.002, respectively), while a borderline significant difference was found in the frequencies of TNF-α (-308) between cases and controls (P=0.063). The variants of three SNPs increased the risk of silicosis in the Chinese workers exposed to silica particles. The adjusted ORs of TNF-α (-308), TNF-α (-238) and IL-1RA (+2018) were 2.8 (95% CI: 1.1-7.5), 20.9 (95% CI: 1.8-236.4) and 4.0 (95% CI: 1.6-10.1), respectively. Conclusion It is suggested that cytokine polymorphisms of TNF- α (-238, -308) and IL-1RA (+2018) are associated with the risk of silicosis in the Chinese workers exposed to silica particles. Further independent studies on the interaction between SNPs and exposure to silica particles with a larger sample size are therefore warranted. | WANG Yong Wei LAN Ya Jia YANG Yue Lin WANG De Jun KUANG Jie | 2012 | Biomedical and Environmental Sciences2012，25，5： | 11 |
| 6 | The formation and evolution of Chepaizi-Mosuowan paleo-uplift and its control on the distributions of sedimentary facies in the Junggar Basin显示文摘The Chepaizi-Mosuowan paleo-uplift is a large-scale uplift stretching across the Junggar Basin formed during the Yanshanian. It has experienced four evolutionary stages: the initial forming stage (J1), the intense development stage (J2+3), the waning and burial stage (K-E), and the tilting and extinction stage (N-Q). The most intense period of activities is the Middle Jurassic. Dur-ing the Early Jurassic, the Chepaizi-Mosuowan paleo-structure was a low amplitude uplift. Because of the subsequent strong uplifting during Middle-Late Jurassic, the Middle and Upper Jurassic were eroded. With the evolution of the Chepaizi-Mosuowan paleo-uplift, the sedimentary pattern of the basin changed, and the paleo-uplift separated the northern depositional systems from those in the south side. As a result, the basin tectonics controlled the distribution and evolution of the depositional systems. During Early Jurassic, while the paleo-uplift was low, its controlling effect on depositional systems was limited and sediments coming from the northwest could reach the central and southern parts of the basin. With the strong uplifting of the Chepaizi-Mosuowan paleo-structure during Middle-Late Jurassic, sediments from the northwest provenance could only deposit in the northern graben of the paleo-uplift. The intense erosion of the Middle-Upper Jurassic also changed the former sedimentary center of the basin into a source area, supplying sediments for grabens on both sides of the paleo-uplift. In the Cretaceous, regional subsidence caused the paleo-uplift to be buried again and subsequently sediments accumulated on top of it. The depositional facies are dominated by fluvial in the Early Cretaceous and shallow lacustrine to deltaic in the Late Cretaceous. In the history of the long-lasting development of this paleo-uplift, large-scale erosions of the paleohigh not only provided sufficient sediments to the center of the Junggar Basin, but also created favorable conditions for the formation of various subtle traps such as lithologic and stratigraphic traps. | JI YouLiang ZHOU Yong KUANG Jun WAN Lu ZHANG Rui LU ChunHong | 2010 | Science China Earth Sciences2010，53，6： | 10 |
| 7 | Microalloying Al alloys with Sc:a review显示文摘As a kind of important light alloys,the Al alloys exhibit mechanical properties that are closely related to the microstructures.Changing the main alloying elements and adjusting heat treatments are usually approaches to tune the microstructure and hence artificially control the mechanical properties.However,the windows for the two approaches have become quite narrow,after extensive studies in the last half of century.Microalloying has become the most promising strategy to further modify the microstructure and improve the mechanical properties of Al alloys,among which the element of scandium(Sc)is especially powerful.In this paper,the recent progresses in Al alloys microalloyed with Sc are briefly reviewed,focusing on the microstructural characterization,strengthening response,and underlying mechanisms.The possible key research points are also proposed for the further development of Al alloys microalloyed with Sc and other rare earth elements. | Jin-Yu Zhang Yi-Han Gao Chong Yang Peng Zhang Jie Kuang Gang Liu Jun Sun | 2020 | Rare Metals2020，39，6： | 10 |
| 8 | The influence of graded levels of available phosphorus on growth performance,muscle antioxidant and flesh quality of young grass carp(Ctenopharyngodon idella)显示文摘Growth, muscle composition, meat quality characteristics and antioxidant capacity in muscle of young grass carp(Ctenopharyngodon idella)(initial weight 282.9 ± 3.3 g) fed graded levels of phosphorus(1.0,2.5, 3.8, 5.6, 7.8 and 9.5 g/kg diet) for 8 wk were investigated. Results indicated that percentage weight gain, feed intake, feed efficiency, serum phosphorus and alkaline phosphatase were improved with optimal phosphorus supplementations(P < 0.05). Muscle protein content and water holding capacity were significantly elevated, while moisture, lipid and ash contents were significantly decreased with dietary phosphorus to a certain level(P < 0.05). The meat shear force value and hydroxyproline content were not influenced by different levels of phosphorus. Muscle anti-hydroxyl radical, superoxide dismutase, catalase, glutathione S-transferase activities and glutathione content were significantly improved(P < 0.05). Conversely, anti-superoxide anion, glutathione reducase and glutathione peroxidase activities were decreased(P < 0.05) with dietary phosphorus to a certain level. These results indicated that suitable dietary phosphorus improved growth performance, meat quality and muscle antioxidant capacity. Dietary available phosphorus requirement of young grass carp for percentage weight gain was4.0 g/kg diet. | Jing Wen Weidan Jiang Lin Feng Shengyao Kuang Jun Jiang Ling Tang Xiaoqiu Zhou Yang Liu | 2015 | Animal Nutrition2015，，2： | 8 |
| 9 | Influence of silane on corrosion resistance of magnesium alloy AZ31 with thermally sprayed aluminum coatings显示文摘Aluminum coatings on Mg alloy AZ31 were fabricated using the thermal spraying technique, and then sealed with silane.The surface morphology and chemical groups were discerned using scanning electron microscopy and examined using Fourier transformation infrared spectroscopy, respectively.The salt fog tests and the potentiodynamic electrochemical technique were applied to evaluate the influence of silane on corrosion of the AZ31 alloy with aluminum coatings.The results showed that the corrosion resistance of the aluminum-coated AZ31 alloy was superior to that of the substrate.The aluminum coating sealed with various silane layers led to a further increase in the corrosion resistance of the alloy.Double silane layers were more corrosion-resistant than the single one.Also, it was no longer significant for more than two silane layers to improve the corrosion resistance.It implied that the optimum choice for silane treatment on the aluminum coatings was two layers. | ZENG Rongchang CHEN Jun KUANG Jun ZHANG Jin WANG Ying | 2010 | Rare Metals2010，29，2： | 7 |
| 10 | Control of Facies and Potential on Jurassic Hydrocarbon Accumulation and Prediction of Favorable Targets in the Hinterland Region of the Junggar Basin显示文摘Exploration practices show that the Jurassic System in the hinterland region of the Junggar Basin has a low degree of exploration but huge potential,however the oil/gas accumulation rule is very complicated,and it is difficult to predict hydrocarbon-bearing properties.The research indicates that the oil and gas is controlled by structure facies belt and sedimentary system distribution macroscopically,and hydrocarbon-bearing properties of sand bodies are controlled by lithofacies and petrophysical facies microscopically.Controlled by ancient and current tectonic frameworks,most of the discovered oil and gas are distributed in the delta front sedimentary system of a palaeo-tectonic belt and an ancient slope belt.Subaqueous branch channels and estuary dams mainly with medium and fine sandstone are the main reservoirs and oil production layers,and sand bodies of high porosity and high permeability have good hydrocarbon-bearing properties;the facies controlling effect shows a reservoir controlling geologic model of relatively high porosity and permeability.The hydrocarbon distribution is also controlled by relatively low potential energy at the high points of local structure macroscopically, while most of the successful wells are distributed at the high points of local structure,and the hydrocarbon-bearing property is good at the place of relatively low potential energy;the hydrocarbon distribution is in close connection with faults,and the reservoirs near the fault in the region of relatively low pressure have good oil and gas shows;the distribution of lithologic reservoirs at the depression slope is controlled by the distribution of sand bodies at positions of relatively high porosity and permeability.The formation of the reservoir of the Jurassic in the Junggar Basin shows characteristics of favorable facies and low-potential coupling control,and among the currently discovered reservoirs and industrial hydrocarbon production wells,more than 90%are developed within the scope of faciespotential index FPI>0.5,while the FPI and oil saturation of the discovered reservoir and unascertained traps have relatively good linear correlation.By establishing the relation model between hydrocarbonbearing properties of traps and FPI,totally 43 favorable targets are predicted in four main target series of strata and mainly distributed in the Badaowan Formation and the Sangonghe Formation,and the most favorable targets include the north and east of the Shinan Sag,the middle and south of the Mobei Uplift,Cai-35 well area of the Cainan Oilfield,and North-74 well area of the Zhangbei fault-fold zone. | CHEN Dongxia PANG Xiongqi KUANG Jun KANG Dejiang LEI Lei DENG Yougen | 2010 | Acta Geologica Sinica(English Edition)2010，84，5： | 7 |
| 11 | Cell cycle-related kinase reprograms the liver immune microenvironment to promote cancer metastasis显示文摘The liver is an immunologically tolerant organ and a common metastatic site of multiple cancer types.Although a role for cancer cell invasion programs has been well characterized,whether and how liver-intrinsic factors drive metastatic spread is incompletely understood.Here,we show that aberrantly activated hepatocyte-intrinsic cell cycle-related kinase(CCRK)signaling in chronic liver diseases is critical for cancer metastasis by reprogramming an immunosuppressive microenvironment.Using an inducible liverspecific transgenic model,we found that CCRK overexpression dramatically increased both B16F10 melanoma and MC38 colorectal cancer(CRC)metastasis to the liver,which was highly infiltrated by polymorphonuclear-myeloid-derived suppressor cells(PMNMDSCs)and lacking natural killer T(NKT)cells.Depletion of PMN-MDSCs in CCRK transgenic mice restored NKT cell levels and their interferon gamma production and reduced liver metastasis to 2.7% and 0.7%(metastatic tumor weights)in the melanoma and CRC models,respectively.Mechanistically,CCRK activated nuclear factor-kappa B(NF-κB)signaling to increase the PMN-MDSC trafficking chemokine C-X-C motif ligand 1(CXCL1),which was positively correlated with liver-infiltrating PMN-MDSC levels in CCRK transgenic mice.Accordingly,CRC liver metastasis patients exhibited hyperaaivation of hepatic CCRK/NF-κB/CXCL1 signaling,which was associated with accumulation of PMN-MDSCs and paucity of NKT cells compared to healthy liver transplantation donors.In summary,this study demonstrates that immunosuppressive reprogramming by hepatic CCRK signaling undermines antimetastatic immunosurveillance.Our findings offer new mechanistic insights and therapeutic targets for liver metastasis intervention. | Xuezhen Zeng Jingying Zhou Zhewen Xiong Hanyong Sun Weiqin Yang Myth T.S.Mok Jing Wang Jingqing Li Man Liu Wenshu Tang Yu Feng Hector Kwong-Sang W ang Shun-Wa Tsang King-Lau Chow Philip Chun Yeung John Wong Paul Bo-San Lai Anthony Wing-Hung Chan Ka Fai To Stephen Lam Chan Qiang Xia Jing Xue Xiao Chen Jun Yu Sui Peng Joseph Jao-Yiu Sung Ming Kuang Alfred Sze-Lok Cheng | 2021 | Cellular & Molecular Immunology2021，18，4： | 5 |
| 12 | Robust leaderless time-varying formation control for unmanned aerial vehicle swarm system with Lipschitz nonlinear dynamics and directed switching topologies显示文摘This paper tackles the robust leaderless Time-Varying Formation(TVF)control problem for the Unmanned Aerial Vehicle(UAV)swarm system with Lipschitz nonlinear dynamics,external disturbances and directed switching topologies.In comparison with the previous achievements on formation control problems,the UAV swarm system with Lipschitz nonlinear dynamics can accomplish the pre-designed TVF while tracking a pre-given trajectory which is produced by a virtual leader UAV in the presence of external disturbances.Firstly,by applying the consensus theory,a TVF controller is developed with the local neighborhood status information,the errors of real time status of all UAVs,the expected formation configuration and the pre-given trajectory under directed switching topologies.Secondly,through a certain matrix variable substitution,the UAV swarm system formation control issue is transformed into a lower dimensional asymptotically stable control issue.Thirdly,by introducing the minimum dwell time,the design steps of formation control algorithm are further acquired.In the meantime,the stability of the UAV swarm system is analyzed through the construction of a piecewise continuous Lyapunov functional and via the Linear Matrix Inequalities(LMIs)method.Finally,the comparison results of a numerical simulation are elaborated to verify the validity of the proposed approach. | Yuhang KANG Yu KUANG Jun CHENG Bangchu ZHANG Yahui QI Shaolei ZHOU Kai MAO | 2022 | Chinese Journal of Aeronautics2022，35，1： | 4 |
| 13 | Late-Stage Reservoir Formation Effect and Its Dynamic Mechanisms in Complex Superimposed Basins显示文摘复杂的附加的盆展出多级式的构造事件和多级式的水库形成;在早阶段形成的烃水库在经历以后阶段的多重构造事件的调整,重建和破坏以后有通常迟了阶段的开始特征。在这份报纸,这现象被称为迟了阶段的水库形成效果。迟了阶段的水库形成效果在复杂的附加的盆是油和煤气形成的水库的一个基本特征,揭示在附加的盆而且迟了阶段的水库形成的重要性的油和煤气形成的水库的多级式的特性,关联和复杂性不仅。迟了阶段的水库形成不在附加的盆是油和煤气的形成水库的一个基本特征。多级式的水库形成仅仅在附加的盆描绘油和煤气形成的水库的一个方面并且不充分在附加的盆代表油和煤气形成的水库的复杂性。我们用迟了阶段的水库形成效果建议代替迟了阶段的水库形成概念在附加的盆指导复杂水库的探索。在当前的地质的条件下面,迟了阶段的水库形成效果主要被四种基本形式代表:分阶段执行转变,规模重建,部件变化和陷井调整。迟了阶段的水库形成效果被二种地质的过程生产:首先,油和各种各样的地质的阀值(烃逐出阀值,烃迁居阀值,和烃积累阀值)的煤气的保留功能引起油和煤气的水库形成的实际时间比在常规意义的大量烃的产生的时间迟了,生产迟了阶段的水库形成效果;第二,在油和煤气的水库形成以后的构造事件(连续地强壮的重建,早阶段的强壮的重建,中间阶段的强壮的重建,迟了阶段的强壮的重建和长期的稳定的沉积)的多重类型导致更早形成的水库的调整,重建和破坏,并且由于迟了阶段的水库形成效果形成新第二等的烃水库。 | KANG Dejiang PANG Xiongqi KUANG Jun LUO Xiaorong PANG Hong LEI Lei | 2010 | Acta Geologica Sinica(English Edition)2010，84，5： | 4 |
| 14 | Effects of overaging temperature on the microstructure and properties of 600MPa cold-rolled dual-phase steel显示文摘C–Mn steels prepared by annealing at 800°C for 120 s and overaging at 250–400°C were subjected to pre-straining(2%) and baking treatments(170°C for 20 min) to measure their bake-hardening(BH_2) values. The effects of overaging temperature on the microstructure, mechanical properties, and BH_2 behavior of 600 MPa cold-rolled dual-phase(DP) steel were investigated by optical microscopy, scanning electron microscopy, and tensile tests. The results indicated that the martensite morphology exhibited less variation when the DP steel was overaged at 250–350°C. However, when the DP steel was overaged at 400°C, numerous non-martensite and carbide particles formed and yield-point elongation was observed in the tensile curve. When the overaging temperature was increased from 250 to 400°C, the yield strength increased from 272 to 317 MPa, the tensile strength decreased from 643 to 574 MPa, and the elongation increased from 27.8% to 30.6%. Furthermore, with an increase in overaging temperature from 250 to 400°C, the BH_2 value initially increases and then decreases. The maximum BH_2 value of 83 MPa was observed for the specimen overaged at 350°C. | Chun-fu Kuang Zhi-wang Zheng Gong-ting Zhang Jun Chang Shen-gen Zhang Bo Liu | 2016 | International Journal of Minerals,Metallurgy and Materials2016，23，8： | 4 |
| 15 | The relationship between dietary methionine and growth,digestion,absorption,and antioxidant status in intestinal and hepatopancreatic tissues of sub-adult grass carp(Ctenopharyngodon idella)显示文摘Background: Methionine is an essential amino acid for fish. The present study was conducted to investigate the effects of dietary methionine on growth performance, digestive and absorptive ability, as well as antioxidant capacity in the intestine and hepatopancreas of sub-adult grass carp(Ctenopharyngodon idella).Results: Dietary methionine deficiency significantly decreased percentage weight gain(PWG), feed intake, feed efficiency and protein efficiency ratio, as well as activities of hepatopancreatic glutamate-oxaloacetate transaminase and muscle glutamate-pyruvate transaminase in sub-adult grass carp(P < 0.05). Furthermore, methionine deficiency significantly reduced activities of trypsin, lipase and amylase in the intestine, Na^+/K^+-ATPase, alkaline phosphatase and γ-glutamyl transpeptidase in three intestinal segments, and creatine kinase(CK) in the proximal intestine(P < 0.05). However, an unexplained and significant increase in CK activity in the mid intestine was associated with dietary methionine deficiency. Malondialdehyde and protein carbonyl contents in the intestine and hepatopancreas were significantly increased by methionine deficiency(P < 0.05), whereas anti-hydroxyl radical capacity in the hepatopancreas and intestine, and anti-superoxide anion capacity in the intestine, were significantly decreased by methionine deficiency(P < 0.05). Moreover, methionine deficiency significantly decreased superoxide dismutase and glutathione reductase activities, glutathione contents in the hepatopancreas and intestine, as well as glutathione peroxidase activity in the intestine(P < 0.05), whereas it significantly increased activities of catalase in the hepatopancreas and glutathione-S-transferase in the hepatopancreas and intestine(P < 0.05).(Continued on next page)(Continued from previous page)Conclusions: The present results demonstrated that dietary methionine deficiency induced poor growth, and decreased digestive and absorptive function and antioxidant capacity in the hepatopancreas and intestine of sub-adult grass carp. Methionine requirements for sub-adult grass carp(450-1, 170 g) based on PWG, intestinal trypsin, and hepatopancreatic anti-hydroxyl radical activities were estimated to be 6.12 g/kg diet(21.80 g/kg protein),6.99 g/kg diet(24.90 g/kg protein) and 5.42 g/kg diet(19.31 g/kg protein), respectively, in the presence of 1.50 g cysteine/kg(5.35 g/kg protein). | Pei Wu Ling Tang Weidan Jiang Kai Hu Yang Liu Jun Jiang Shengyao Kuang ling tang Wuneng Tang Yongan Zhang Xiaoqiu Zhou Lin Feng | 2017 | Journal of Animal Science and Biotechnology2017，8，4： | 4 |
| 16 | Prostate-specific membrane antigen expression in hepatocellular carcinoma,cholangiocarcinoma,and liver cirrhosis显示文摘BACKGROUND Primary liver cancer includes three subtypes:Hepatocellular carcinoma(HCC),intrahepatic cholangiocarcinoma(CCA),and combined hepatocellular carcinoma.Patients with primary liver cancer experienced poor prognosis and high mortality,so early detection of liver cancer and improved management of metastases are both key strategies to reduce the death toll from liver cancer.Prostate-specific membrane antigen(PSMA)expression in the tumor-associated neovasculature of nonprostate malignancies including liver cancer has been reported recently,but conclusive evidence of PSMA expression based on the pathological type of liver cancer remains limited.AIM To study the expression of PSMA in HCC,CCA,and liver cirrhosis.METHODS A total of 446 formalin-fixed paraffin-embedded(FFPE)liver tumor and liver cirrhosis tissue samples were obtained retrospectively from the Pathology Department of Tongji Hospital.Immunohistochemistry was used to detect PSMA expression in these 446 FFPE liver biopsy specimens(213 HCC,203 CCA,and 30 liver cirrhosis).The tumor compartment and the associated neovascular endothelium were separately analyzed.PSMA expression was examined by two certified pathologists,and the final results were presented in a 4-point scoring system(0-3 points).Correlation between PSMA expression and clinicopathological information was also assessed.RESULTS PSMA was expressed primarily in the neovascular endothelium associated with tumors.The positive rate of PSMA staining in HCC was significantly higher than that in CCA(86.8%vs 79.3%;P=0.001)but was only 6.6%in liver cirrhosis(P=0.000).HCC cases had more 3-score PSMA staining than CCA had(89/213,41.8%vs 35/203,17.2%;P=0.001).PSMA expression correlated positively with the stage and grade of HCC and CCA.In both liver cancer subtypes,there were more PSMA+cases in stages III–V diseases than in stages I and II.High staining intensity of PSMA was more frequently observed in liver cancers at high grade and advanced stage.There was no significant association of PSMA expression with sex,age,region,α-fetoprotein,hepatitis B surface antigen,or tumor size in both tumor subtypes.CONCLUSION Neovascular PSMA may be a promising marker to differentiate HCC from liver cirrhosis and a prognostic marker for anti-tumor angiogenesis therapy for HCC. | Li-Xing Chen Si-Juan Zou Dan Li Jian-Yuan Zhou Zhao-Ting Cheng Jun Zhao Yuan-Li Zhu Dong Kuang Xiao-Hua Zhu | 2020 | World Journal of Gastroenterology2020，26，48： | 3 |
| 17 | Growth,digestive and absorptive capacity and antioxidant status in intestine and hepatopancreas of sub-adult grass carp Ctenopharyngodonidella fed graded levels of dietary threonine显示文摘Background:This study was carried out to investigate effects of threonine levels on growth,digestive and absorptive capacity and antioxidant status in intestine and hepatopancreas of sub-adult grass carp(Ctenopharyngodonidella).Results:Weight gain,specific growth rate,feed intake and feed efficiency were significantly improved by dietary threonine(P<0.05).Intestinal activities of trypsin,chymotrypsin,alpha-amylase,lipase,alkaline phosphatase,y-glutamyl transpeptidase and creatine kinase took the similar trends.Contents of malondialdehyde and protein carbonyl in intestine and hepatopancreas were significantly decreased by dietary optimal threonine supplementation(P<0.05).Anti-superoxide anion capacity,anti-hydroxyl radical capacity,glutathione content and activities of superoxide dismutase,catalase and glutathione-S-transferase in intestine and hepatopancreas were enhanced by dietary threonine(P< 0.05).Conclusions:Dietary threonine could improve growth,enhance digestive and absorptive capacity and antioxidant status in intestine and hepatopancreas of sub-adult grass carp.The dietary threonine requirement of sub-adult grass carp(441.9-1,013.4 g) based on weight gain was 11.6 g/kg diet or 41.5 g/kg of dietary protein by quadratic regression analysis. | Yang Hong Weidan Jiang Shengyao Kuang Kai Hu Ling Tang Yang Liu Jun Jiang Yongan Zhang Xiaoqiu Zhou Lin Feng | 2015 | Journal of Animal Science and Biotechnology2015，6，4： | 3 |
| 18 | Abnormal brain activity in adolescents with Internet addiction who attempt suicide: an assessment using functional magnetic resonance imaging显示文摘Internet addiction is associated with an increased risk of suicidal behavior and can lead to brain dysfunction among adolescents.However,whether brain dysfunction occurs in adolescents with Internet addiction who attempt suicide remains unknown.This observational cross-sectional study enrolled 41 young Internet addicts,aged from 15 to 20 years,from the Department of Psychiatry,the First Affiliated Hospital of Chongqing Medical University,China from January to May 2018.The participants included 21 individuals who attempted suicide and 20 individuals with Internet addiction without a suicidal attempt history.Brain images in the resting state were obtained by a 3.0 T magnetic resonance imaging scanner.The results showed that activity in the gyrus frontalis inferior of the right pars triangularis and the right pars opercularis was significantly increased in the suicidal attempt group compared with the non-suicidal attempt group.In the resting state,the prefrontal lobe of adolescents who had attempted suicide because of Internet addiction exhibited functional abnormalities,which may provide a new basis for studying suicide pathogenesis in Internet addicts.The study was authorized by the Ethics Committee of Chongqing Medical University,China(approval No.2017 Scientific Research Ethics(2017-157))on December 11,2017. | Yan Huang Lu Xu Li Kuang Wo Wang Jun Cao Mu-Ni Xiao | 2020 | Neural Regeneration Research2020，15，8： | 3 |
| 19 | SARS-CoV-2 spike protein interacts with and activates TLR41显示文摘Dear Editor,Accumulating clinical data suggest the main causes of death by COVID-19 include respiratory failure and the onset of sepsis.1 Importantly,sepsis has been observed in nearly all deceased patients.2,3,4,5 It remains elusive how SARS-CoV-2 infection results in viral sepsis in humans.Toll-like receptor 4(TLR4)mediates anti-gram-negative bacterial immune responses by recognizing lipopolysaccharide(LPS)from bacteria.6 We recently found that SARS-CoV-2 infection provoked an anti-bacterial like response at the very early stage of infection via TLR4.However,the identity of the original trigger initiating these abnormal immune responses during SARS-CoV-2 infection is unknown. | Yingchi Zhao Ming Kuang Junhong Li Ling Zhu Zijing Jia Xuefei Guo Yaling Hu Jun Kong Hang Yin Xiangxi Wang Fuping You | 2021 | Cell Research2021，31，7： | 3 |
| 20 | Effects of pre-strain and baking parameters on the microstructure and bake-hardening behavior of dual-phase steel显示文摘In a typical process, C-Mn steel was annealed at 800°C for 180 s, and then cooled rapidly to obtain the ferrite-martensite microstructure. After pre-straining, the specimens were baked and the corresponding bake-hardening(BH) values were determined as a function of pre-strain, baking temperature, and baking time. The influences of pre-strain, baking temperature and baking time on the microstructure evolution and bake-hardening behavior of the dual-phase steel were investigated systematically. It was found that the BH value apparently increased with an increase in pre-strain in the range from 0 to 1%; however, increasing pre-strain from 1% to 8% led to a decrease in the BH value. Furthermore, an increase in baking temperature favored a gradual improvement in the BH value because of the formation of Cottrell atmosphere and the precipitation of carbides in both the ferrite and martensite phases. The BH value reached a maximum of 110 MPa at a baking temperature of 300°C. Moreover, the BH value enhanced significantly with increasing baking time from 10 to 100 min. | Chun-fu Kuang Shen-gen Zhang Jun Li Jian Wang Hua-fei Liu | 2014 | International Journal of Minerals,Metallurgy and Materials2014，21，8： | 2 |
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