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| 1 | Characterization and pilot functional study of a root-specific MYB transcription factor of Arabidopsis显示文摘An MYB transcription factor (AtMYB305) of Arabidopsis was structurally characterized. The biochemical activity of the protein was tested and its in vivo function was preliminarily analyzed. AtMYB305 contains two imperfect repeats (R2, R3) in its DNA binding domain. Gel mobility shift assay demonstrated that GST-AtMYB305 fusion pro-tein bound specifically to the DNA fragment that included a consensus MYB recognition sequence (TAACTG). Overex-pression of AtMYB305 in the fission yeast caused the forma-tion of elongated cells with one condensed nucleus. Semi-quantitative RT-PCR analysis revealed that AtMYB305 was expressed specifically in the roots of Arabidopsis. | Dong Wang Lihong Li Zhiling Chen Guixian Xia | 2002 | Chinese Science Bulletin2002，47，4： | 4 |
| 2 | Identification of HSC/MPP expansion units in fetal liver by single-cell spatiotemporal transcriptomics显示文摘Limited knowledge of cellular and molecular mechanisms underlying hematopoietic stem cell and multipotent progenitor(HSC/MPP)expansion within their native niche has impeded the application of stem cell-based therapies for hematological malignancies.Here,we constructed a spatiotemporal transcriptome map of mouse fetal liver(FL)as a platform for hypothesis generation and subsequent experimental validation of novel regulatory mechanisms.Single-cell transcriptomics revealed three transcriptionally heterogeneous HSC/MPP subsets,among which a CD93-enriched subset exhibited enhanced stem cell properties.Moreover,by employing integrative analysis of single-cell and spatial transcriptomics,we identified novel HSC/MPP‘pocket-like’units(HSC PLUS),composed of niche cells(hepatoblasts,stromal cells,endothelial cells,and macrophages)and enriched with growth factors.Unexpectedly,macrophages showed an 11-fold enrichment in the HSC PLUS.Functionally,macrophage-HSC/MPP co-culture assay and candidate molecule testing,respectively,validated the supportive role of macrophages and growth factors(MDK,PTN,and IGFBP5)in HSC/MPP expansion.Finally,cross-species analysis and functional validation showed conserved cell-cell interactions and expansion mechanisms but divergent transcriptome signatures between mouse and human FL HSCs/MPPs.Taken together,these results provide an essential resource for understanding HSC/MPP development in FL,and novel insight into functional HSC/MPP expansion ex vivo. | Suwei Gao Qiang Shi Yifan Zhang Guixian Liang Zhixin Kang Baofeng Huang Dongyuan Ma Lu Wang Jianwei Jiao Xiangdong Fang Cheng-Ran Xu Longqi Liu Xun Xu Berthold Gottgens Cheng Li Feng Liu | 2022 | Cell Research2022，32，1： | 4 |
| 3 | Expression of a cotton profilin gene GhPFN1 is associated with fiber cell elongation显示文摘Profilin is an important actin-binding protein involved in regulating the organization of actin filaments. A cotton profilin gene (GhPFN1) that shares 71% identity to profilin1 of Arabidopsis in its amino acid sequence was isolated. Semi-quantitative RT-PCR showed that GhPFN1 was expressed preferentially in the developing cotton fibers and reached the highest level at the fast elongation stage. The function of GhPFN1 in vivo was analyzed using the S. pombe system, and results suggested that GhPFN1 plays a role in fiber cell elongation. | LI Lihong, WANG Haiyun, LI Yan and XIA Guixian(Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China) | 2002 | Progress in Natural Science:Materials International2002，12，10： | 3 |
| 4 | Research on meshing theory of noninvolute beveloid gears 显示文摘 | LI GUIXIAN WEN JIANMIN | 2003 | Journal of Harbin Institate of Technology2003，10，2： | 1 |
| 5 | A large insert Thellungiella halophila BIBAC library for genomics and identification of stress tolerance genes显示文摘 | Weiquan Wang Yaorong Wu Yin Li Jiaying Xie Zhonghui Zhang Zhiyong Deng Yiyue Zhang Cuiping Yang Jianbin Lai Huawei Zhang Hongyan Bao Sanyuan Tang Chengwei Yang Peng Gao Guixian Xia Huishan Guo Qi Xie | 2010 | Plant Molecular Biology (-)2010，，1： | 1 |
| 6 | Study of Catalytic Hydrogenation of 1, 4-Benzenediol over Ru/HY catalys显示文摘 | Li Guixian Dong Peng Wang Xiaorui | 2011 | Journal of Molecular Catalysis (China)2011，15，4： | 1 |
| 7 | New progress of Keggin and Wells-Dawson type polyoxometalates catalyze acid and oxidative reactions 显示文摘 | Li Guixian Ding Yong Wang Jianming | 2007 | Journal of Molecular Catalysis A: Chemical2007，262，12： | 1 |
| 8 | Mono-substituted Keggin-polyoxometalate complexes as effective and recyclable catalyst for the oxidation of alcohols with hydrogen peroxide in biphasic system显示文摘 | Jianmin Wang Liang Yan Guixian Li Xiaolai Wang Yong Dings and Jishuan Suo | 2005 | Tetrahedron Letters2005，46，： | 1 |
| 9 | Newprogress of Keggin and Wells-Dawson type polyoxometa-lates catalyze acid and oxidative reactions 显示文摘 | Li Guixian Ding Yong Wang Jianming | 2007 | Journalof Molecular Catalysis A Chemical2007，262，： | 1 |
| 10 | Meching Analysis on the Quasi-ellipsoidal Gear Transmission with inner Toroidal Teeth in the Flexible Joint of Robot显示文摘 | Li Guixian Li Huamin Bi Zhuming | 1992 | Chinese Joumal of Mechanical Engeering1992，5，： | 1 |
| 11 | Wells-Dawson Type Molybdovanadophosphoric Heteropolyacids Catalyzed Prins Cyclization of Alkenes with Paraformaldehyde Under Mild Conditions: A Facile and Efficient Method to 1,3-Dioxane Derivatives 显示文摘 | Li Guixian Gu Yanlong Ding Yong | 2004 | J Mol Catal A : Chem2004，218，2： | 1 |
| 12 | Direct Side-Chain Oxidation of Ethylbenzene over Supported Co4HP2Mo15V3O62Catalysts as a Clean and Highly Effi cient Approach to Producing Acetophenone显示文摘 | Li Guixian Li Yazhen Mu Ruina | 2013 | React Kinet Mech Catal2013，109，1： | 1 |
| 13 | New progress of Keggin and Wells-Dawson type polyoxometalates catalyze acid and oxidative reactions 显示文摘 | Li Guixian Ding Yong Wang Jianming | 2007 | Journal of Molecular Catalysis A: Chemical2007，262，： | 1 |
| 14 | Kinetic Study of the Esterifi cation of Acetic Acid and n-Amyl Alcohol Catalyzed by H3PW6Mo6O40 Immobilized on Silylated Palygorskite显示文摘 | Li Guixian Mu Ruina Fan Zongliang | 2013 | React Kinet Mech Catal2013，110，1： | 1 |
| 15 | Pyridine-keggin heteropoly compounds as catalyst for hydroxylation of phenol using hydrogen peroxide as oxidant显示文摘 | Jianmin Wang Liang Yan Guang Qian Gaomeng Lv Guixian Li Jishuan Suo Xiaolai Wang | 2007 | Reaction Kinetics and Catalysis Letters2007，，1： | 1 |
| 16 | New pro- gress of Keggin and Wells-Dawson type polyoxometalates catalyze acid and oxidative reactions 显示文摘 | LI Guixian DING Yong WANG Jianming | 2007 | Journal of Mo- lecular Catalysis A: Chemical2007，262，12： | 1 |
| 17 | New Progress of Keggin and Wells - Dawson type Polyoxometalates catalyze acid and oxidative reactions 显示文摘 | Guixian Li Yong Ding Jianming Wang | 2007 | Journal of Molecular Catalysis A2007，262，2： | 1 |
| 18 | Quasi Ellipsoid Gear Surface Reconstruction Based on Meshless Local Petrov-Galerkin Method and Transmission Characteristic显示文摘Special transmission 3D model simulation must be based on surface discretization and reconstruction,but special transmission usually has complicated tooth shape and movement,so present software can't provide technical support for special transmission 3D model simulation.Currently,theoretical calculation and experimental method are difficult to exactly solve special transmission contact analysis problem.How to reduce calculation and computer memories consume and meet calculation precision is key to resolve special transmission contact analysis problem.According to 3D model simulation and surface reconstruction of quasi ellipsoid gear is difficulty,this paper employes meshless local Petrov-Galerkin(MLPG) method.In order to reduce calculation and computer memories consume,we disperse tooth mesh into finite points-sparseness points cloud or grid mesh,and then we do interpolation reconstruction in some necessary place of the 3D surface model during analysis.Moving least square method(MLSM) is employed for tooth mesh interpolation reconstruction,there are some advantages to do interpolation by means of MLSM,such as high precision,good flexibility and no require of tooth mesh discretization into units.We input the quasi ellipsoid gear reconstruction model into simulation software,we complete tooth meshing simulation.Simulation transmission ratio during meshing period was obtained,compared with theoretical transmission ratio,the result inosculate preferably.The method using curve reconstruction realizes surface reconstruction,reduce simulation calculation enormously,so special gears simulation can be realized by minitype computer.The method provides a novel solution for special transmission 3D model simulation analysis and contact analysis. | WU Xuemei SHAN Debin LI Guixian | 2010 | Chinese Journal of Mechanical Engineering2010，23，6： | 1 |
| 19 | Catalytic oxidation of toluene tobenzaldehyde over keggin-type heteropolyacid compounds显示文摘 | Li Guixian Wang Guang Gao Yunyan | 2010 | Fine Chem2010，28，1： | 1 |
| 20 | New Progressof Keggin and Wells-Dawson Type Polyoxometalates CatalyzeAcid and Oxidative Reactions显示文摘 | Li Guixian Ding Yong Wang Jianming | 2007 | J Mol Cata A:Chem2007，262，12： | 1 |
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