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| 1 | Detection of serum TNF-α,IFN-γ,IL-6 and IL-8 in patients with hepatitis B显示文摘NTRODUCTIONSinceMuto[1]reportedthatTNFαandIL1wererelatedtofulminanthepatitis,thestudiesontherelationshipbetweencytokinesa... | WANG Jing Yan, WANG Xue Lian and LIU Pei | 1999 | World Journal of Gastroenterology1999，5，1： | 54 |
| 2 | Infection with novel coronavirus(SARS-CoV-2)causes pneumonia in Rhesus macaques显示文摘The 2019 novel coronavirus(SARS-CoV-2)outbreak is a major challenge for public health.SARS-CoV-2 infection in human has a broad clinical spectrum ranging from mild to severe cases,with a mortality rate of^6.4%worldwide(based on World Health Organization daily situation report).However,the dynamics of viral infection,replication and shedding are poorly understood.Here,we show that Rhesus macaques are susceptible to the infection by SARS-CoV-2.After intratracheal inoculation,the first peak of viral RNA was observed in oropharyngeal swabs one day post infection(1 d.p.i.),mainly from the input of the inoculation,while the second peak occurred at 5 d.p.i.,which reflected on-site replication in the respiratory tract.Histopathological observation shows that SARS-CoV-2 infection can cause interstitial pneumonia in animals,characterized by hyperemia and edema,and infiltration of monocytes and lymphocytes in alveoli.We also identified SARS-CoV-2 RNA in respiratory tract tissues,including trachea,bronchus and lung;and viruses were also re-isolated from oropharyngeal swabs,bronchus and lung,respectively.Furthermore,we demonstrated that neutralizing antibodies generated from the primary infection could protect the Rhesus macaques from a second-round challenge by SARS-CoV-2.The non-human primate model that we established here provides a valuable platform to study SARS-CoV-2 pathogenesis and to evaluate candidate vaccines and therapeutics. | Chao Shan Yan-Feng Yao Xing-Lou Yang Yi-Wu Zhou Ge Gao Yun Peng Lian Yang Xue Hu Jin Xiong Ren-Di Jiang Hua-Jun Zhang Xiao-Xiao Gao Cheng Peng Juan Min Ying Chen Hao-Rui Si Jia Wu Peng Zhou Yan-Yi Wang Hong-Ping Wei Wei Pang Zheng-Fei Hu Long-Bao Lv Yong-Tang Zheng Zheng-Li Shi Zhi-Ming Yuan | 2020 | Cell Research2020，30，8： | 19 |
| 3 | Diagnostic performance of magnifying narrow-band imaging for early gastric cancer: A meta-analysis显示文摘AIM: To investigate the performance of magnifying endoscopy with narrow-band imaging(ME-NBI) in the diagnosis of early gastric cancer(EGC).METHODS: Systematic literature searches were conducted until February 2014 in Pub Med, EMBASE, Web of Science, Ovid, Scopus and the Cochrane Library databases by two independent reviewers. Meta-analysis was performed to calculate the pooled sensitivity, specificity and diagnostic odds ratio and to construct a summary receiver operating characteristic(ROC) curve. Subgroup analyses were performed based on the morphology type of lesions, diagnostic standard, the size of lesions, type of assessment, country and sample size to explore possible sources of heterogeneity. A Deeks' asymmetry test was used to evaluate the publication bias.RESULTS: Fourteen studies enrolling 2171 patients were included. The pooled sensitivity, specificity and diagnostic odds ratio for ME-NBI diagnosis of EGC were 0.86(95%CI: 0.83-0.89), 0.96(95%CI: 0.95-0.97) and 102.75(95%CI: 48.14-219.32), respectively, with the area under ROC curve being 0.9623. Among the 14 studies, six also evaluated the diagnostic value of conventional white-light imaging, with a sensitivityof 0.57(95%CI: 0.50-0.64) and a specificity of 0.79(95%CI: 0.76-0.81). When using 'VS'(vessel plus surface) ME-NBI diagnostic systems in gastric lesions of depressed macroscopic type, the pooled sensitivity and specificity were 0.64(95%CI: 0.52-0.75) and 0.96(95%CI: 0.95-0.98). For the lesions with a diameter less than 10 mm, the sensitivity and specificity were 0.74(95%CI: 0.65-0.82) and 0.98(95%CI: 0.97-0.98).CONCLUSION: ME-NBI is a promising endoscopic tool in the diagnosis of early gastric cancer and might be helpful in further target biopsy. | Ying-Ying Hu Qing-Wu Lian Zheng-Hua Lin Jing Zhong Meng Xue Liang-Jing Wang | 2015 | World Journal of Gastroenterology2015，21，25： | 18 |
| 4 | Syntheses and Fungicidal Activities of Thiazole-5-carboxanilides Bearing Thioether Group显示文摘 | XUE Hansong LIU Aiping LIU Weidong LI Jianming REN Yeguo HUANG Lu HE Lian OU Xiaoming YE Jiao HUANG Mingzhi | 2016 | Chemical Research in Chinese Universities2016，32，5： | 13 |
| 5 | Stem cells in gastric cancer显示文摘Gastric cancer(GC) is one of the leading causes of cancerrelated mortality worldwide.Cancer stem cells(CSCs),which were first identified in acute myeloid leukemia and subsequently in a large array of solid tumors,play important roles in cancer initiation,dissemination and recurrence.CSCs are often transformed tissue-specific stem cells or de-differentiated transit amplifying progenitor cells.Several populations of multipotent gastric stem cells(GSCs) that reside in the stomach have been determined to regulate physiological tissue renewal and injury repair.These populations include the Villin+ and Lgr5+ GSCs in the antrum,the Troy+ chief cells in the corpus,and the Sox2+ GSCs that are found in both the antrum and the corpus.The disruption of tumor suppressors in Villin+ or Lgr5+ GSCs leads to GC in mouse models.In addition to residing GSCs,bone marrow-derived cells can initiate GC in a mouse model of chronic Helicobacter infection.Furthermore,expression of the cell surface markers CD133 or CD44 defines gastric CSCs in mouse models and in human primary GC tissues and cell lines.Targeted elimination of CSCs effectively reduces tumor size and grade in mouse models.In summary,the recent identification of normal GSCs and gastric CSCs has greatly improved our understanding of the molecular and cellular etiology of GC and will aid in the development of effective therapies to treat patients. | Ying-Ying Hu Qing-Wu Lian Zheng-Hua Lin Jing Zhong Meng Xue Liang-Jing Wang | 2015 | World Journal of Gastroenterology2015，21，1： | 10 |
| 6 | Characterization of submicron aerosols in the urban outflow of the central Pearl River Delta region of China显示文摘 | Zhaoheng GONG Zijuan LAN Lian XUE Liwu ZENG Lingyan HE Xiaofeng HUANG | 2012 | Frontiers of Environmental Science & Engineering2012，6，5： | 9 |
| 7 | Construction and anti-tumor effects of recombinant fowlpox virus expressing Newcastle disease virus hemagglutinin-neuramidinase gene显示文摘Hemagglutinin-neuramidinase (HN ) ,纽卡斯尔疾病导出病毒的蛋白质,不是仅仅调停受体识别而且拥有 neuraminidase (NA ) 活动,劈开那些受体的一个部件的能力, N-acetylneuraminic 酸(NAcneu, sialic ) 。包括人,在哺乳动物的种类的这蛋白质有有趣的反肿瘤以及有免疫力的刺激性质,这被知道。在癌症基因治疗探索 HN 基因的使用,我们构造了表示 HN 蛋白质(vFV-HN ) 的一个 recombinant 家禽痘病毒并且在 vivo 并且在 vitro 把 recombinant 病毒的 theanti 肿瘤活动与野类型的家禽痘病毒(FPV ) 的作比较。这里,我们发现尽管 B16 房间对野类型的家禽痘病毒的基础细胞毒素的效果有点抵抗,有 vFV-HN 的感染引起了显著细胞毒素的效果并且,与 vFV-HN 使免疫的忍受肿瘤的老鼠的幸存显著地与独自与 FPV 使免疫的老鼠的幸存相比被增加。而且,有 vFV-HNelicited 的老鼠的免疫 B16 肿瘤特定的细胞毒素的 T 淋巴细胞(CTL ) 回答和在 vivo 的 bothCD4+ 和 CD8+ T 房间人口的同种细胞的扩大。另外,从老鼠的淋巴节点的 T 房间种牛痘, Th1 cytokine IL-2 和 IFN-v 高级的 vFV-HN 藏匿了,显示肿瘤房间的回归与 Th1 类型主导的有免疫力的回答有关。这些结果证明有 vFV-HN 的种痘可以是为癌症基因治疗的潜在的策略。 | LI Xiao JIN Ningyi LIAN Hai GUAN Goufang SUN Lili LI Xue mei ZHENG Hongling | 2006 | Chinese Science Bulletin2006，51，22： | 9 |
| 8 | Molecular epidemiology of serotype 19A Streptococcus pneumoniae isolated from children in Beijing, 1997-2006显示文摘尽管有链球菌 pneumoniae serotype 19A 的流行的背景,这 serotype 的分子的特征充分还有待于被阐明。学习因此是在打字的 China.Methods 脉搏地胶化电气泳动和 multilocus 顺序决定 serotype 19A 的相同的这的目的被做到这 49 serotype 19A 孤立调查在在北京和另外的区域流行的紧张之间的关系。从 1997 ~ 2006 的结果, serotype 19A 的百分比孤立增加。到青霉素和 amoxicillin 的危险性率减少了,到 cefuroxime 的抵抗率增加了。ST320 是最流行的圣,由 ST3546 列在后面。有六新圣,在我们的学习识别。serotype 19A 种类被分类进六不同脉搏地胶化电气泳动(PFGE ) 模式。 ST320 ,与二个不同 PFGE 模式( A 和 D )被联系,说明了因为 32 孤立,并且 ST3546 ,与二个 PFGE 模式( B 和 E )被联系,向前从 2003 说明了 eightConclusions , ST320 是最普通的圣和到显著地增加的 cefuroxime 的抵抗的率。链球菌 pneumoniae serotype 19A 的进一步长期的调查被要求在这重要人的病原体监视圣流行和抗菌剂抵抗。 | XUE Lian YAO Kai-hu YU Sang-jie LIU Zun-jie QIAN Jing SHEN Xu-zhuang YANG Yong-hong | 2011 | Chinese Medical Journal2011，，12： | 6 |
| 9 | Expression of proliferating cell nuclear antigen in polyps from large intestine显示文摘Coloncancerhasahighincidenceintheworld,especialyinWesterncountries,andtheincidenceisalsoincreasinginChinainrecentyears.Toredu... | LUO Yu Qin 1, MA Lian Sheng 2, ZHAO Yi Ling 3, WU Kai Chun 4, PAN Bo Rong 5 and ZHANG Xue Yong 4 | 1999 | World Journal of Gastroenterology1999，5，2： | 6 |
| 10 | The role and clinical implications of microRNAs in hepatocellular carcinoma显示文摘Hepatocellular carcinoma (HCC) is common and one of the most aggressive of all human cancers. Recent studies have indicated that miRNAs, a class of small noncoding RNAs that regulate gene expression post-transcriptionally, directly contribute to HCC by targeting many critical regulatory genes. Several miRNAs are involved in hepatitis B or hepatitis C virus replication and virus-induced changes, whereas others participate in multiple intracellular signaling pathways that modulate apoptosis, cell cycle checkpoints, and growth-factor-stimulated responses. When disturbed, these pathways appear to result in malignant transformation and ultimately HCC development. Recently, miRNAs circulating in the blood have acted as possible early diagnostic markers for HCC. These miRNA also could serve as indicators with respect to drug efficacy and be prognostic in HCC patients. Such biomarkers would assist stratification of HCC patients and help direct personalized therapy. Here, we summarize recent advances regarding the role of miRNAs in HCC development and progression. Our expectation is that these and ongoing studies will contribute to the understanding of the multiple roles of these small noncoding RNAs in liver tumorigenesis. | ZHAO Xue YANG Zhen LI GuangBing LI DongKai ZHAO Yi WU Yan ROBSON Simon C. HE Lian XU YiYao MIAO R-uoYu ZHAO HaiTao | 2012 | Science China(Life Sciences)2012，55，10： | 6 |
| 11 | A series of Er3+-activated SrLaGa3O7 single crystal fibers for mid-infrared laser application显示文摘A multidisciplinary approach for the production and characterization of a series of high concentration Er3+activated SrLaGa3 O7(abbreviated as Er:SLGO)crystal fibers is shown to have a great promise for implementation in mid-infrared laser applications.The current approach includes the design and formation of unique layered tetrahedral network structures with several kinds of rare earth(RE)ions including Er ions distributing statistically between layers,such as Er:SLGO,Er,Nd:SLGO,Er,Yb,Ho:SLGO,Er,Eu:SLGO and Er,Ho:SLGO.Five kinds of Er:SLGO crystal fibers were designed to grow via a micropulling down method.Spectroscopic analyses show that Er,Yb,Ho:SLGO and Nd,Er:SLGO crystal fibers were superiorly endowed with inhomogeneous broadening absorption and strong emission.The unique structural components design enables the generation of improved absorption and emission recombination,and the inhibition of self-termination as well.Generally,the use of structural components design may warrant high-efficiency emissions in RE-doped crystal fibers. | Yan Wang Yingshu Lian Yanping Zhang Chaoyang Tu Dongfeng Xue | 2020 | Journal of Rare Earths2020，38，5： | 5 |
| 12 | Characteristics of deep drainage and soil water in the mobile sandy lands of Inner Mongolia, northern China显示文摘Quantification of deep drainage and the response of soil water content to rainfall patterns are critical for an effective management strategy of soil water conservation and groundwater utilization. However, there has been little information on how rainfall characteristics influence soil water dynamics and deep drainage in mobile sandy lands. We used an underground chamber to examine the responses of deep drainage and soil water content in mobile sandy lands to rainfall characteristics in Inner Mongolia during the growing seasons of 2010, 2011 and 2012. Results showed that rainfall in this area was dominated by small events(≤5 mm), which increased soil water content in the surface soil layers(0–40 cm), but did not increase soil water content in the deeper soil layers(greater than 40 cm). Soil water content at the 0–100 cm depth increased significantly when the total amount of rain was >20 mm. Rainfall amount, intensity and the duration of dry intervals were significantly related to the soil water content in different soil layers. Deep drainage was significantly correlated with rainfall amount and intensity, but not with the duration of dry intervals. The coefficients of deep drainage in the mobile sandy lands ranged from 61.30% to 67.94% during the growing seasons. Our results suggested that rainfall infiltration in the mobile sandy lands had considerable potential to increase soil water storage while recharging the groundwater in this region. | Xin Ping LIU Yu Hui HE Xue Yong ZHAO Tong Hui ZHANG La Mei ZHANG Yun Hua MA Shu Xia YAO Shao Kun WANG Shui Lian WEI | 2015 | Journal of Arid Land2015，7，2： | 4 |
| 13 | MicroRNA-182 promotes pancreatic cancer cell proliferation and migration by targeting β-TrCP2显示文摘胰腺的癌症是好攻击的恶意。中部的幸存率仍然保持低,显示新奇 biomarkers 和治疗学的目标的鉴定是批评的。这里,我们在胰腺的癌症开发检验了 microRNA-182 (miR-182 ) 的角色。人的胰腺的癌症标本和房间线的分析证明 miR-182 是在胰腺的癌症的 overexpressed 并且支持肿瘤增长和侵略。-TrCP2 作为 miR-182 的一个直接目标被证实。-TrCP2 的 Silencing 增加了类似于 miR-182 overexpression 的 -catenin, 的层次。-TrCP2 的宫外的表示禁止了 -catenin 发信号的 miR-182-induced 激活。由 -TrCP2 的 miR-182 和它的颠倒的 oncogenic 效果在 vivo 被证实。这研究建议 -TrCP 和 miR-182 可以为胰腺的癌症的早察觉和治疗是可能的 biomarkers 和目标。 | Shi Wang Jiansong Ji Jingjing Song Xue Li Shilong Han Weishuai Lian Chuanwu Cao Xiaoping Zhang Maoquan Li | 2016 | Acta Biochimica et Biophysica Sinica2016，48，12： | 4 |
| 14 | Effects of luteinizing hormone and androgen on the development of rat progenitor Leydig cells in vitro and in vivo显示文摘祖先 Leydig 房间从干细胞被导出。祖先 Leydig 房间的增长和区别显著地在发身期间贡献 Leydig 房间数字。然而,这些过程的规定仍然保持不清楚。现在的学习的目的是决定 luteinizing 荷尔蒙(LH ) 或雄激素是否贡献祖先 Leydig 房间的增长和区别。Fourteen-day-old 男 Sprague-Dawley 老鼠与 NalGlu 被对待 7 天,它是一个释放 gonadotropin 荷尔蒙对手,到在垂体并且这样减少 LH 的分泌物,在睾丸的雄激素。老鼠与 LH 或 7 α 被共同管理; -methyl-nortestosterone (MENT ) 是对由 5 α 的新陈代谢抵抗的雄激素, -reductase 1 在祖先 Leydig 房间,和 LH 或雄激素的随后的效果被测量。3 H-Thymidine 静脉内地也被注入老鼠在祖先 Leydig 房间学习 thymidine 加入。祖先 Leydig 房间被检验。NalGlu 管理由 83 % 减少了祖先 Leydig 房间增长;。另外, LH 或 MENT 处理恢复了 Leydig 房间 proliferative 能力到 73 %或 50 %控制,分别地。增长相关的基因的送信人 RNA 层次用即时 PCR 被测量。Igf1, Lifr, Pdgfra, Bcl2, Ccnd3 和 Pcna 的表示层次是由 MENT 的 upregulated,并且 Pdgfra, Ccnd3 和 Pcna 的那些是由 LH 的 upregulated。LH 和 MENT 在 vitro 刺激了祖先 Leydig 房间的区别。我们断定 LH 和 MENT 涉及调整祖先 Leydig 房间的发展。 | Jing-Jing Guo Xue Ma Claire Q F Wang Yu-Fei Ge Qing-Quan Lian Dianne O Hard Yu-Fei Zhang Qiang Dong Yun-Fei Xu Ren-Shan Ge | 2013 | Asian Journal of Andrology2013，15，5： | 4 |
| 15 | Evodiamine activates cellular apoptosis through suppressing PI3K/AKT and activating MAPK in glioma显示文摘OBJECTIVE Glioblastoma multiforme(GBM) is the most malignant primary tumor of the central nervous system and is associated with a very poor prognosis.No further improvements in outcomes have been reported since radiotherapy-temozolomide therapy was introduced.Therefore,de.veloping new agents to treat GBM is important.This study aimed to evaluate the anti-tumor effect of evodiamine(Evo) on GBM cells,and to determine the underlying mechanisms involved.METHODS U251,LN229,HEB and PC12 cells were treated with various concentrations of evodiamine for 24 and48 hours,cell viability was measured by MTT assay.The U251 and LN229 cells were treated with evo.diamine(0-10 μmol·L^(-1)) for 24 h,and then stained with Hoechst 33258.An Annexin V-FITC Apoptosis Detection Kit was used to detect apoptosis in the cells.Reactive oxygen species(ROS) production was detected using dichlorofluorescein diacetate(DCFH-DA) staining.The changes in mitochondrial mem.brane potential(MMP) were assessed by JC-1 after cells were treated with evodiamine.The expres.sion levels of p-PI3K,PI3K,p-Akt,Akt,Bax,Bcl-2,p-p38,p38,p-JNK,JNK,p-ERK,ERK,Cytochrome c,Caspase-3,cleaved Caspase-3,PRAP,and cleaved PARP were measured by Western blot analy.ses.RESULTS According to MTT assay results,Evo significantly inhibited the cell proliferation in a time-and dose-dependent manner.Fluorescence microscopy and flow cytometry analyses revealed that Evo induced cell apoptosis in a concentration-dependent manner.Moreover,Evo induced reactive oxygen species(ROS) production and mitochondrial membrane potential(MMP) disruption.Finally,Evo induced apoptosis in cancer cells by suppressing PI3K/AKT signaling and inducing MAPK phos.phorylation(p38 and JNK,but not ERK) to regulate apoptotic proteins(Bax,Bcl-2,Cytochrome c,Cas.pase-3,and PARP).CONCLUSION In summary,Evo inhibits cell proliferation by inducing cellular apoptosis via suppressing PI3K/AKT and activating MAPK in GBM;these results indicate that Evo may be regarded as a new approach for GBM treatment. | Feng ZHI Rong WANG Dan-hi DENG Nai-yuan SHAO Yuan XU Lian XUE Ya PENG Ya-tian LIU | 2018 | 中国药理学与毒理学杂志2018，32，4： | 4 |
| 16 | Protective Efficacy of Inactivated Vaccine against SARS-CoV-2 Infection in Mice and Non-Human Primates显示文摘The ongoing coronavirus disease 2019(COVID-19)pandemic caused more than 96 million infections and over 2 million deaths worldwide so far.However,there is no approved vaccine available for severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the disease causative agent.Vaccine is the most effective approach to eradicate a pathogen.The tests of safety and efficacy in animals are pivotal for developing a vaccine and before the vaccine is applied to human populations.Here we evaluated the safety,immunogenicity,and efficacy of an inactivated vaccine based on the whole viral particles in human ACE2 transgenic mouse and in non-human primates.Our data showed that the inactivated vaccine successfully induced SARS-CoV-2-specific neutralizing antibodies in mice and non-human primates,and subsequently provided partial(in low dose)or full(in high dose)protection of challenge in the tested animals.In addition,passive serum transferred from vaccine-immunized mice could also provide full protection from SARS-CoV-2 infection in mice.These results warranted positive outcomes in future clinical trials in humans. | Yan-Feng Yao Ze-Jun Wang Ren-Di Jiang Xue Hu Hua-Jun Zhang Yi-Wu Zhou Ge Gao Ying Chen Yun Peng Mei-Qin Liu Ya-Nan Zhang Juan Min Jia Lu Xiao-Xiao Gao Jing Guo Cheng Peng Xu-Rui Shen Qian Li Kai Zhao Lian Yang Xin Wan Bo Zhang Wen-Hui Wang Jia Wu Peng Zhou Xing-Lou Yang Shuo Shen Chao Shan Zhi-Ming Yuan Zheng-Li Shi | 2021 | Virologica Sinica2021，36，5： | 4 |
| 17 | Immunological Evaluation of a Novel Mycobacterium tuberculosis Antigen Rv0674显示文摘Objective This study aimed to characterize the diagnostic and vaccine potential of a novel Mycobacterium tuberculosisantigen Rv0674. Methods To evaluate thediagnostic potential and antigenicity of Rv0674, IgG was evaluated using ELISA and interferon (IFN)-γ was done by using ELISpot assay among TB patients and healthy donors. For immunogenicity evaluation, BALB/c mice were immunized with Rv0674. Cytokine production was determined by cytokine release assay using an ELISA kit, and the antibodies were tested using ELISA. Results The results of serum Elisa tests showed that Rv0674 specific immunoglobulin G (IgG) response was higher in TB patients than negative controls. And Rv0674 had good performance in serological test with sensitivity and specificity of 77.1% and 81.1%, respectively. While it shows poor sensitivity and specificity of 26.23% and 79.69% for IFN-γ tests. In BALB/c mice, Rv0674 adjuvant by DDA/PolyI:C could also induce a high level of IFN-γ, interleukin-2 and interleukin-6 as well as a high IgG titer in both high-and low-dose groups indicating that Rv0674 is essential in humoral and cellular immunity. Moreover, the cytokine profile and IgG isotypecharacterized Rv0674 as a Th1/Th2-mixed-type protective immunity with the predominance of Th1 cytokines. Conclusion Rv0674 may be a good potential candidate for the development of TB serological diagnosis and a new TB vaccine. | XIAO Tong Yang LIU Hai Can LI Xiao Qin HUANG Ming Xiang LI Gui Lian LI Na YAN Yu Han LUO Qiao WANG Xue Zhi LI Ma Chao WAN Kang Lin | 2019 | Biomedical and Environmental Sciences2019，32，6： | 3 |
| 18 | Restrictive Cardiomyopathy Resulting from a Troponin Ⅰ Type 3 Mutation in a Chinese Family显示文摘Objective To identify the pathogenic variant responsible for restrictive cardiomyopathy(RCM) in a Chinese family.Methods Next generation sequencing was used for detecting the mutation and results verified by sequencing.We used restriction enzyme digestion to test the mutation in the family members and 200 unrelated normal subjects without any cardiac inherited diseases when the mutation was identified.Results Five individuals died from cardiac diseases,two of whom suffered from sudden cardiac death.Two individuals have suffered from chronic cardiac disorders.Mutation analysis revealed a novel missense mutation in exon 7 of troponin Ⅰ type 3(TNNI3),resulting in substitution of serine(S) with proline(P) at amino acid position 150,which cosegregated with the disease in the family,which is predicted to be probably damaging using PolyPhen-2.The mutation was not detected in the 200 unrelated subjects we tested.Conclusion Using next generation sequencing,which has very recently been shown to be successful in identifying novel causative mutations of rare Mendelian disorders,we found a novel mutation of TNNI3 in a Chinese family with RCM. | Yan-ping Ruan Chao-xia Lu Xiao-yi Zhao Rui-juan Liang Hui Lian Michael Routledge Wei Wu Xue Zhang Zhong-jie Fan | 2016 | Chinese Medical Sciences Journal2016，31，1： | 3 |
| 19 | Hypoxia-induced GBE1 expression promotes tumor progression through metabolic reprogramming in lung adenocarcinoma显示文摘Hypoxia mediates a metabolic switch from oxidative phosphorylation to glycolysis and increases glycogen synthesis.We previously found that glycogen branching enzyme(GBE1)is downstream of the hypoxia-inducible factor-1(HIF1)signaling pathway in lung adenocarcinoma(LUAD)cells;however,the molecular mechanism underlying HIF1 regulation of GBE1 expression remains unknown.Herein,the effect of GBE1 on tumor progression via changes in metabolic signaling under hypoxia in vitro and in vivo was evaluated,and GBE1-related genes from human specimens and data sets were analyzed.Hypoxia induced GBE1 upregulation in LUAD cells.GBE1-knockdown A549 cells showed impaired cell proliferation,clone formation,cell migration and invasion,angiogenesis,tumor growth,and metastasis.GBE1 mediated the metabolic reprogramming of LUAD cells.The expression of gluconeogenesis pathway molecules,especially fructose-1,6-bisphosphatase(FBP1),was markedly higher in shGBE1 A549 cells than it was in the control cells.FBP1 inhibited the tumor progression of LUAD.GBE1-mediated FBP1 suppression via promoter methylation enhanced HIF1αlevels through NF-κB signaling.GBE1 may be a negative prognostic biomarker for LUAD patients.Altogether,hypoxia-induced HIF1αmediated GBE1 upregulation,suppressing FBP1 expression by promoter methylation via NF-κB signaling in LUAD cells.FBP1 blockade upregulated HIF1α,triggered the switch to anaerobic glycolysis,and enhanced glucose uptake.Therefore,targeting HIF1α/GBE1/NF-κB/FBP1 signaling may be a potential therapeutic strategy for LUAD. | Lifeng Li Li Yang Zhirui Fan Wenhua Xue Zhibo Shen Yongliang Yuan Xiangdong Sun Dan Wang Jingyao Lian Liping Wang Jie Zhao Yi Zhang | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 3 |
| 20 | Diagnostic Value of Cerebrospinal Fluid T-SPOT.TB for Tuberculousis Meningitis in China显示文摘The aim of this study was to evaluate the diagnostic value of the cerebrospinal fluid(CSF) T‐SPOT.TB test for the diagnosis of TB meningitis(TBM). A retrospective analysis of 96 patients with manifested meningitis was conducted; T‐SPOT.TB test was performed for diagnosing TBM to determine the diagnostic sensitivity, specificity, positive predictive value(PPV), and negative predictive value(NPV). A receiver operating characteristic(ROC) curve was also drawn to assess the diagnostic accuracy. The sensitivity, specificity, PPV, and NPV of CSF T‐SPOT.TB test were 97.8%, 78.0%, 80.3%, and 97.5%, respectively, for 52 patients(54.2%) of the 96 enrolled patients. The area under the curve(AUC) was 0.910, and the sensitivities of CSF T‐SPOT.TB for patients with stages I, II, and III of TBM were 96.7%, 97.2%, and 98.9%, respectively. CSF T‐SPOT.TB test is a rapid and accurate diagnostic method with higher sensitivity and specificity for diagnosing TBM. | LI Xue Lian XIE Na WANG Song Wang WU Qian Hong MA Yan SHU Wei CHEN Hong Mei ZHANG Li Qun WU Xiao Guang MA Li Ping CHE Nan Ying GAO Meng Qiu | 2017 | Biomedical and Environmental Sciences2017，30，9： | 3 |
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