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| 1 | Doublecortin-like kinase 1 exhibits cancer stem cell-like characteristics in a human colon cancer cell line显示文摘Purpose: Colon cancer stem cells (CSCs) are implicated in colorectal cancer carcinogenesis, metastasis, and therapeutic resistance. The identification of these cells could help to develop novel therapeutic strategies. Doublecortin-like kinase 1 (DCLK1) has been viewed as a marker for gastrointestinal stem cells that fuel the self-renewal process, however others view them as a marker of Tuft cells or as an enteroendocrine subtype. The purpose of this study was to use a colon cancer cell line to identify and characterize the stem-like characteristics of the DCLK1+ cell population. Methods: To enrich stem-like cells, HCT116 cells (derived from colon adenocarcinomas) were cultured using serum-free media to form spheres under both normal oxygen and hypoxia condition. DCLK1 transcript expression in the adherent parental cells and spheroids was quantified using quantitative real time reverse transcription- polymerase chain reaction [(q)RT-PCR]. DCLK1 protein expression was determined using flow cytometry. Self-renewal capability from adherent parental cells and spheroids was determined using extreme limiting dilution analysis (ELDA). Results: Under both normal oxygen and hypoxia condition, the adherent parental cells were composed of cells that express low levels of DCLK1. However, spheroids exhibited an increased frequency of cells expressing DCLK1 on both mRNA and protein levels. Cells derived from spheroids also possess stronger self-renewal capability. Conclusions: The higher fraction of DCLK1+ cells exhibited by spheroids and hypoxia reflects the stem-like characteristics of these cells. DCLK1 may represent an ideal marker to study and develop effective strategies to overcome chemo-resistance and relapse of colon cancer. | Lianna Li Charles F. Bellows | 2013 | Chinese Journal of Cancer Research2013，25，2： | 9 |
| 2 | Single-crystal microplates of two-dimensional organic-inorganic lead halide layered perovskites for optoelectronics显示文摘器官无机的混合 perovskites 在高效率的太阳能电池和轻排放由于他们的应用吸引可观的注意。与三维的 perovskites 相比,二维(2D ) 分层的混合 perovskites 有一个更高的激子绑定精力和潜在地更高的轻排放的效率。因为他们能是为综合光电子的合适的积木,有明确的 nanoscale 形态学的高质量的水晶的 2D perovskites 的生长是合乎需要的并且(nano ) photonics。此处,我们基于 2-phenylethylammonium 报导 2D perovskites 的单个水晶的 microplates 的灵巧的答案生长( C 6 H 5 CH 2 CH 2 NH 3+,豌豆)阳离子,(豌豆) 2 PbX 4( X = Br ,我),与通过一个溶解再结晶过程的明确的矩形的几何学和 nanoscale 厚度。水晶结构(豌豆)2 PbX 4 首先用单个水晶的 X 光检查衍射被证实。一个答案阶段运输生长过程被开发在与与铅醋酸盐先锋电影涂的底层不同的另一干净底层上与几百纳米的十测微计和厚度的一种典型尺寸种 microplates。表面地形学分析建议 2D microplates 的形成被婚礼蛋糕生长机制多半驾驶。通过卤化物 alloying,光致发光排放(豌豆)2 Pb (Br,我) 有狭窄的山峰带宽的 4 perovskites 乐意地被调节从紫(~ 410 nm ) 到绿色(~ 530 nm ) 。 | Dewei Ma Yongping Fu Lianna Dang Jianyuan Zhai Ilia A. Guzei Song Jin | 2017 | Nano Research2017，10，6： | 2 |
| 3 | Simultaneous determination of active xanthone glycosides, timosaponins and alkaloids in rat plasma after oral administration of Zi-Shen Pill extract for the pharmacokinetic study by liquid chromatography–tandem mass spectrometry显示文摘 | Fei Cai Wen Xu Hua Wei Lianna Sun Shouhong Gao Qi Yang Jin Feng Feng Zhang Wansheng Chen | 2010 | Journal of Chromatography B2010，，21： | 1 |
| 4 | Apolipoprotein E polymorphism influences orthotopic liver transplantation outcomes in patients with hepatitis C virus-induced liver cirrhosis显示文摘BACKGROUND Hepatitis C virus(HCV)infection is responsible for a chronic liver inflammation,which may cause end-stage liver disease and hepatocellular carcinoma.Apolipoprotein E(protein:ApoE,gene:APOE),a key player in cholesterol metabolism,is mainly synthesized in the liver and APOE polymorphisms may influence HCV-induced liver damage.AIM To determine whether APOE alleles affect outcomes in HCV-infected patients with liver cirrhosis following orthotopic liver transplantation(OLT).METHODS This was a cohort study in which 179 patients,both genders and aged 34-70 years,were included before or after(up to 10 years follow-up)OLT.Liver injury severity was assessed using different criteria,including METAVIR and models for endstage liver disease.APOE polymorphisms were analyzed by quantitative real-time polymerase chain reaction.RESULTS The APOE3 allele was the most common(67.3%).In inflammation severity of biopsies from 89 OLT explants and 2 patients in pre-transplant,the degree of severe inflammation(A3F4,0.0%)was significantly less frequent than in patients with minimal and moderate degree of inflammation(≤A2F4,16.2%)P=0.048,in patients carrying the APOE4 allele when compared to non-APOE4.In addition,a significant difference was also found(≤A2F4,64.4%vs A3F4,0.0%;P=0.043)and(A1F4,57.4%vs A3F4,0.0%;P=0.024)in APOE4 patients when compared to APOE3 carriers.The fibrosis degree of the liver graft in 8 of 91 patients and the lack of the E4 allele was associated with more moderate fibrosis(F2)(P=0.006).CONCLUSION Our results suggest that the E4 allele protects against progression of liver fibrosis and degree of inflammation in HCV-infected patients. | José Carlos Rodrigues Nascimento Lianna C Pereira Juliana Magalhães C Rêgo Ronaldo P Dias Paulo Goberlânio B Silva Silvio Alencar C Sobrinho Gustavo R Coelho Ivelise Regina C Brasil Edmilson F Oliveira-Filho James S Owen Pierluigi Toniutto Reinaldo B Oriá | 2021 | World Journal of Gastroenterology2021，27，11： | 1 |
| 5 | A genome-wide comparison of genes responsive to autopolyploidy inIsatis indigotica usingArabidopsis thaliana Affymetrix genechips显示文摘 | Beibei Lu Xinzhu Pan Lei Zhang Beibei Huang Lianna Sun Bin Li Bo Yi Shuiqing Zheng Xiaojing Yu Ruxian Ding Wansheng Chen | 2006 | Plant Molecular Biology Reporter2006，，2： | 1 |
| 6 | Interplay between Two Epigenetic Marks显示文摘 | Lianna M. Johnson Xiaofeng Cao Steven E. Jacobsen | 2002 | Current Biology2002，，16： | 1 |
| 7 | Inhibitory effect of traditional Chinese medicine Zi-Shen Pill on benign prostatic hyperplasia in rats 显示文摘 | He S TieJun L LianNa S | 2008 | Journal of Ethnopharmacology2008，15，2： | 1 |
| 8 | A simple and sensitive HPLC method for the simultaneous determination of eight bioactive components and fingerprint analysis of Schisandra sphenanthera显示文摘 | Hua Wei Lianna Sun Zongguang Tai Shouhong Gao Wen Xu Wansheng Chen | 2010 | Analytica Chimica Acta2010，，1： | 1 |
| 9 | Characterization and expression profiling of tyrosine aminotransferase gene from Salvia miltiorrhiza (Dan-shen) in rosmarinic acid biosynthesis pathway显示文摘 | Beibei Huang Bo Yi Yanbing Duan Lianna Sun Xiaojing Yu Jing Guo Wansheng Chen | 2008 | Molecular Biology Reports2008，，4： | 1 |
| 10 | DEMETER, a DNA Glycosylase Domain Protein, Is Required for Endosperm Gene Imprinting and Seed Viability in Arabidopsis显示文摘 | Yeonhee Choi Mary Gehring Lianna Johnson Mike Hannon John J. Harada Robert B. Goldberg Steven E. Jacobsen Robert L. Fischer | 2002 | Cell2002，，1： | 1 |
| 11 | A simple and sensi- tive HPLC method for the simultaneous determination of eight bio- active components and fingerprint analysis of Schisandra sphenan- thera显示文摘 | Wei Hua Sun Lianna Tai Zongguang | 2010 | Anal Chim Acta2010，662，： | 1 |
| 12 | A genome-wide comparison of genes responsive to autopolyploidy inIsatis indigotica usingArabidopsis thaliana Affymetrix genechips显示文摘 | Beibei Lu Xinzhu Pan Lei Zhang Beibei Huang Lianna Sun Bin Li Bo Yi Shuiqing Zheng Xiaojing Yu Ruxian Ding Wansheng Chen | 2006 | Plant Molecular Biology Reporter2006，，2： | 1 |
| 13 | Characterization and expression profiling of tyrosine aminotransferase gene from Salvia miltiorrhiza (Dan-shen) in rosmarinic acid biosynthesis pathway显示文摘 | Beibei Huang Bo Yi Yanbing Duan Lianna Sun Xiaojing Yu Jing Guo Wansheng Chen | 2008 | Molecular Biology Reports2008，，4： | 1 |
| 14 | Screening of target genes and regulatory function of miRNAs as prognostic indicators for prostate cancer显示文摘 | Xiaoli Z Yawei W Lianna L | 2015 | Medical science monitor: international medical joumal of experimental and clinical research2015，，21： | 1 |
| 15 | The SRA methyl-cytosine-binding domain links DNA and histone methylation 显示文摘 | LIANNA M J MAGNOLIA B STEVEN E J | 2007 | Current Biology2007，17，： | 1 |
| 16 | Metabotropic receptors in excitotoxicity: ( S )-4-carboxy-3-hydroxyphenylglycine (( S )-4C3HPG) protects against rat striatal quinolinic acid lesions显示文摘 | Lianna R. Orlando David G. Standaert John B. Penney Anne B. Young | 1995 | Neuroscience Letters1995，，1： | 1 |
| 17 | Epilepsy, suicidality, and psychiatric disorders: A bidirectional association显示文摘 | Dale C. Hesdorffer Lianna Ishihara Lakshmi Mynepalli David J. Webb John Weil W. Allen Hauser | 2012 | Ann Neurol2012，，2： | 1 |
| 18 | 电子时代游戏当道显示文摘在电脑软件飞速发展的时代,电子游戏也经历了多次变革。如今,一个全新的时代已经来临,它带来了最酷的游戏模式,最逼真动感的幻想天地。 | Lianna | 2008 | 上海百货2008，，9： | 0 |
| 19 | 用蜜奶和水果量肤现配显示文摘 众多的媒体曝光事件使许多顾客开始担心化妆品的质量,这无疑给美容院老板出了一道难题.引进新产品后,怎么做才能让顾客对产品质量放心呢?…… | Lianna | 2007 | 医学美学美容(美容师)2007，，2： | 0 |
| 20 | 上海新开美容美发店要听证显示文摘近日,上海市新出台《服务行业行政许可听证办法》,规定本市新开美容美发店必须进行听证,听证会的结果将成为行政许可审批部门作出许可决定的依据。 | Lianna | 2007 | 医学美学美容(财智)2007，，1： | 0 |
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