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| 1 | Introduction of rice chitinase gene into wheat via low energy Ar^+ beam implantation显示文摘A chitinase gene (RCH8) in plasmid vector pCAMBIA1308 was delivered into 3 wheat cultivars (Yangmai 158, Wan 9210, Wanmai 32) by low energy Ar+ beam-mediated method. Preliminary calli from treated mature embryos were first selected on hygromycin (Hm, 20 or 30 mg/L) containing medium. After the resistant calli formed, they were transferred to the regeneration medium with 10 or 20 mg/L Hm. All the three wheat varieties obtained trans-genie plants. PCR and PCR-Southern assays showed that most plants regenerated from the resistant calli were positive transgenic plants. Southern blot of the positive green plants confirmed stable integration of alien DNA into wheat genome. The plant transformation frequencies varied with the variety and ion dose implanted. Wanmai 32 possessed the highest transformation frequency, reaching 3.8% at a suitable implantation dose. The transformation frequency of Yangmai 158 and Wan 9210 varied from 0.5% to 2.5% and from 0.5% to 1.4%, respectively. Progeny test for resistance to | WU Lifang LI Hong FENG Huiyun WU Lijun YU Zengliang | 2001 | Chinese Science Bulletin2001，46，4： | 14 |
| 2 | Advances in COVID-19 mRNA vaccine development显示文摘To date,the coronavirus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has determined 399,600,607 cases and 5,757,562 deaths worldwide.COVID-19 is a serious threat to human health globally.The World Health Organization(WHO)has declared COVID-19 pandemic a major public health emergency.Vaccination is the most effective and economical intervention for controlling the spread of epidemics,and consequently saving lives and protecting the health of the population.Various techniques have been employed in the development of COVID-19 vaccines.Among these,the COVID-19 messenger RNA(mRNA)vaccine has been drawing increasing attention owing to its great application prospects and advantages,which include short development cycle,easy industrialization,simple production process,flexibility to respond to new variants,and the capacity to induce better immune response.This review summarizes current knowledge on the structural characteristics,antigen design strategies,delivery systems,industrialization potential,quality control,latest clinical trials and real-world data of COVID-19 mRNA vaccines as well as mRNA technology.Current challenges and future directions in the development of preventive mRNA vaccines for major infectious diseases are also discussed. | Enyue Fang Xiaohui Liu Miao Li Zelun Zhang Lifang Song Baiyu Zhu Xiaohong Wu Jingjing Liu Danhua Zhao Yuhua Li | 2022 | Signal Transduction and Targeted Therapy2022，7，4： | 5 |
| 3 | Cytosolic delivery of the immunological adjuvant PolyⅠ:C and cytotoxic drug crystals via a carrier-free strategy significantly amplifies immune response显示文摘Co-delivery of chemotherapeutics and immunostimulant or chemoimmunotherapy is an emerging strategy in cancer therapy.The precise control of the targeting and release of agents is critical in this methodology.This article proposes the asynchronous release of the chemotherapeutic agents and immunostimulants to realize the synergistic effect between chemotherapy and immunotherapy.To obtain a proof-of-concept,a co-delivery system was prepared via a drug-delivering-drug(DDD)strategy for cytosolic co-delivery of Poly I:C,a synthetic ds RNA analog to activate RIG-I signaling,and PTX,a commonly used chemotherapeutics,in which pure PTX nanorods were sequentially coated with Poly I:C and mannuronic acid via stimulating the RIG-I signaling axis.The co-delivery system with a diameter of 200 nm enables profound immunogenicity of cancer cells,exhibiting increased secretion of cytokines and chemokines,pronounced immune response in vivo,and significant inhibition of tumor growth.Also,we found that intracellularly sustained release of cytotoxic agents could elicit the immunogenicity of cancer cells.Overall,the intracellular asynchronous release of chemotherapeutics and immunomodulators is a promising strategy to promote the immunogenicity of cancer cells and augment the antitumor immune response. | Xiaoqing Du Yuqi Hou Jia Huang Yan Pang Chenlu Ruan Wei Wu Chenjie Xu Hongwei Zhang Lifang Yin Wei He | 2021 | Acta Pharmaceutica Sinica B2021，11，10： | 3 |
| 4 | Association of chromosome 7 aneuploidy measured by fluorescence in situ hybridization assay with muscular invasion in bladder cancer显示文摘Background:The preoperative prediction of muscular invasion status is important for adequately treating bladder cancer(BC)but nevertheless,there are some existing dilemmas in the current preoperative diagnostic accuracy of BC with muscular invasion.Here,we investigated the potential association between the fluorescence in situ hybridization(FISH)assay and muscular invasion among patients with BC.A cytogenetic-clinical nomogram for the individualized preoperative differentiation of muscle-invasive BC(MIBC)from non-muscle-invasive BC(NMIBC)is also proposed.Methods:All eligible BC patients were preoperatively tested using a FISH assay,which included 4 sites(chromosome-specific centromeric probe[CSP]3,7,and 17,and gene locus-specific probe[GLP]-p16 locus).The correlation between the FISH assay and BC muscular invasion was evaluated using the Chi-square tests.In the training set,univariate and multivariate logistic regression analyses were used to develop a cytogenetic-clinical nomogram for preoperative muscular invasion prediction.Then,we assessed the performance of the nomogram in the training set with respect to its discriminatory accuracy and calibration for predicting muscular invasion,and clinica usefulness,which were then validated in the validation set.Moreover,model comparison was set to evaluate the discrimination and clinical usefulness between the nomogram and the individual variables incorporated in the nomogram.Results:Muscular invasion was more prevalent in BC patients with positive CSP3,CSP7 and CSP17 status(OR[95%CI],2.724[1.555 to 4.774],P<0.001;3.406[1.912 to 6.068],P<0.001 and 2.483[1.436 to 4.292],P=0.001,respectively).Radiologydetermined tumor size,radiology-determined clinical tumor stage and CSP7 status were identified as independent risk factors of BC muscular invasion by the multivariate regression analysis in the training set.Then,a cytogenetic-clinical nomogram incorporating these three independent risk factors was constructed and was observed to have satisfactory discrimination in the training(AUC 0.784;95%CI:0.715 to 0.853)and validation(AUC 0.743;95%CI:0.635 to 0.850)set.The decision curve analysis(DCA)indicated the clinical usefulness of our nomogram.In models comparison,using the receiver operator characteristic(ROC)analyses,the nomogram showed higher discriminatory accuracy than any variables incorporated in the nomogram alone and the DCAs also identified the nomogram as possessing the highest net benefits at wide range of threshold probabilities.Conclusion:CSP7 status was identified as an independent factor for predicting muscular invasion in BC patients and was successfully incorporated in a clinical nomogram combining the results of the FISH assay with clinical risk factors. | Xiayao Diao Jinhua Cai Junjiong Zheng Jianqiu Kong Shaoxu Wu Hao Yu Hao Huang Weibin Xie Xiong Chen Chengran Huang Lifang Huang Haide Qin Jian Huang Tianxin Lin | 2020 | Cancer Communications2020，40，4： | 3 |
| 5 | A survey of routing techniques for satellite networks显示文摘Satellite networks have many advantages over traditional terrestrial networks.However,it is very difficult to design a satellite network with excellent performance.The paper briefly summarizes some existing satellite network routing technologies from the perspective of both single-layer and multilayer satellite constellations,and focuses on the main ideas,characteristics,and existing problems of these routing technologies.For single-layer satellite networks,two routing strategies are discussed,virtual node strategy and virtual topology strategy.Moreover,considering the deficiency of existing multilayer satellite network routing,we discuss the topic invulnerability.Finally,the challenges and problems faced by the satellite network are analyzed and the trend of future development is predicted. | QI Xiaogang MA Jiulong WU Dan LIU Lifang HU Shaolin | 2016 | Journal of Communications and Information Networks2016，1，4： | 3 |
| 6 | Tuning the luminous properties and optical thermometry of Cs_(2)SnCl_(6) phosphor microcrystals via Bi and Sb codoping显示文摘The toxicity and instability of lead halide perovskite seriously limit its commercial application in lighting,although it has high photoluminescence(PL)efficiency and adjustable emission.Here,lead-free bismuth(Bi)and antimony(Sb)codoped Cs_(2)SnCl_(6)(BSCSC)microcrystals(MCs)are prepared successfully by a solvothermal method.The PL spectrum is composed of dual emission bands with the peak at 485 and 650 nm,of which relative intensity can be tunable through the change of Bi and Sb feeding contents,respectively.Because of the phonon–electron interaction,the PL intensity is enhanced as the temperature rises within the range of 80–260 K.Then,the nonradiative transition is intensified until 380 K,which results in decrease in PL intensity.Simultaneously,combining with time-resolved PL,it is concluded that the emission peak at 485 nm is attributed to the[Bi_(Sn)+V_(Cl)]as the luminescent centers with the lifetime of hundreds of nanoseconds,and the emission peak at 650 nm is attributed to microsecond-timescale self-trapped excitons.The maximum values of relative sensitivity(S_(R))and absolute sensitivity(S_(A))values obtained are 3.82%K^(-1) and 5.11 ns·K^(-1),which for the first time to our knowledge demonstrate that BSCSC MCs can be novel luminescent materials for developing better optical thermometry.Whitelight-emitting diodes(WLEDs)are constructed using BSCSC MCs only combined with an LED chip,the Commission Internationale de L’Eclairage color coordinates of which are(0.30,0.37).It provides a novel scheme for the lighting field to realize WLEDs without adding additional commercial phosphors. | SHUNFA GONG RUIRUI WU SEN YANG LIFANG WU MINMIN ZHANG QIUJU HAN WENZHI WU | 2021 | Photonics Research2021，9，11： | 2 |
| 7 | Metal phenolic network-stabilized nanocrystals of andrographolide to alleviate macrophage-mediated inflammation in-vitro显示文摘Macrophages play a crucial role in initiating,maintaining,and resolving inflammation through the phenotypic shift,inducing or inhibiting the production of inflammatory cytokines.Therefore,macrophages are potential targets for treating inflammatory diseases.Andrographolide(AND)is a potent anti-inflammatory drug that can reduce pro-inflammatory cytokines and suppress NF-κB/MAPK pathway in activated macrophages.Although AND has many medicinal properties,its lower water solubility and first-pass effect in the liver have hindered its clinical application.In this context,by using a metal phenolic network as a stabilizer,we designed and prepared highly stabilized AND nanocrystals(AND-MPN Ns)with high drug loading capacity to facilitate the clinical application of AND.Our findings showed that AND-MPN Ns could be used to enhance the anti-inflammation in-vitro via macrophage polarization,reducing proinflammatory cytokines IL-6 and TNF-α,and suppressing the NF-κB signaling pathway activation.The results demonstrated the potential of AND-MPN Ns to combat inflammatory diseases effectively. | Kosheli Thapa Magar George Frimpong Boafo Makhloufi Zoulikha Xiaohong Jiang Xiaotong Li Qingqing Xiao Xuyang Xing Xiaochun Wang Lifang Fan Zhenfeng Wu Wei He | 2023 | Chinese Chemical Letters2023，34，1： | 2 |
| 8 | All-in-One Deposition to Synergistically Manipulate Perovskite Growth for High-Performance Solar Cell显示文摘Nonradiative recombination losses originating from crystallographic distortions and issues occurring upon interface formation are detrimental for the photovoltaic performance of perovskite solar cells.Herein,we incorporated a series of carbamide molecules(urea,biuret,or triuret)consisting of both Lewis base(-NH2)and Lewis acid(-C=O)groups into the perovskite precursor to simultaneously eliminate the bulk and interface defects.Depending on the different coordination ability with perovskite component,the incorporated molecules can either modify crystallization dynamics allowing for large crystal growth at low temperature(60℃),associate with antisite or undercoordinated ions for defect passivation,or accumulate at the surface as an energy cascade layer to enhance charge transfer,respectively.Synergistic benefits of the above functions can be obtained by rationally optimizing additive combinations in an all-in-one deposition method.As a result,a champion efficiency of 21.6%with prolonged operational stability was achieved in an inverted MAPbI3 perovskite solar cell by combining biuret and triuret additives.The simplified all-in-one fabrication procedure,adaptable to different types of perovskites in terms of pure MAPbI3,mixed perovskite,and all-inorganic perovskite,provides a cost-efficient and reproducible way to obtain high-performance inverted perovskite solar cells. | Yifan Lv Hui Zhang Jinpei Wang Libao Chen Lifang Bian Zhongfu An Zongyao Qian Guoqi Ren Jie Wu Frank Nuesch Wei Huang | 2020 | Research2020，，1： | 2 |
| 9 | Radiobiological effects of a lowenergy ion beam on wheat显示文摘 | Lifang Wu Zengliang Yu | 2001 | Radiation Environmental Biophysics2001，40，： | 1 |
| 10 | A review on the GaN-on-Si power electronic devices显示文摘The past decades have witnessed a tremendous development of GaN-based power electronic devices grown on Si substrate.This article provides a concise introduction,review,and outlook of the research developments of GaN-on-Si power device technology.The comprehensive review has discussed the crucial issues in the state-of-the-art device technology based on both GaN materials epitaxy including stress control and point defects study,and device fabrication including normally offsolutions like Cascode,trench MIS-gate,and p-GaN gate.Device reliability and other common fabrication issues in GaN high electron mobility transistors(HEMTs)are also discussed.Lastly,we give an outlook on the GaN-on-Si power devices from two aspects,namely high frequency,and high power GaN ICs,and GaN vertical power devices. | Yaozong Zhong Jinwei Zhang Shan Wu Lifang Jia Xuelin Yang Yang Liu Yun Zhang Qian Sun | 2022 | Fundamental Research2022，2，3： | 1 |
| 11 | Fibrous MXene Aerogels with Tunable Pore of Contaminated Seawater显示文摘The seawater desalination based on solardriven interfacial evaporation has emerged as a promising technique to alleviate the global crisis on freshwater shortage.However,achieving high desalination performance on actual,oil-contaminated seawater remains a critical challenge,because the transport channels and evaporation interfaces of the current solar evaporators are easily blocked by the oil slicks,resulting in undermined evaporation rate and conversion efficiency.Herein,we propose a facile strategy for fabricating a modularized solar evaporator based on flexible MXene aerogels with arbitrarily tunable,highly ordered cellular/lamellar pore structures for high-efficiency oil interception and desalination.The core design is the creation of 1D fibrous MXenes with sufficiently large aspect ratios,whose superior flexibility and plentiful link forms lay the basis for controllable 3D assembly into more complicated pore structures.The cellular pore structure is responsible for effective contaminants rejection due to the multi-sieving effect achieved by the omnipresent,isotropic wall apertures together with underwater superhydrophobicity,while the lamellar pore structure is favorable for rapid evaporation due to the presence of continuous,large-area evaporation channels.The modularized solar evaporator delivers the best evaporation rate(1.48 kg m-2h-1)and conversion efficiency(92.08%)among all MXene-based desalination materials on oil-contaminated seawater. | Fan Wu Siyu Qiang Xiao-Dong Zhu Wenling Jiao Lifang Liu Jianyong Yu Yi-Tao Liu Bin Ding | 2023 | Nano-Micro Letters2023，15，5： | 1 |
| 12 | Coolant choice for the central beryllium pipe of the BES Ⅲ beam pipe显示文摘 | ZHENG Lifang WANG Li WU Ping | 2010 | Chin Phys C2010，34，7： | 1 |
| 13 | Analysis of global gene expression profiles to identify differentially expressed genes critical for embryo development in Brassica rapa显示文摘 | Yu Zhang Lifang Peng Ya Wu Yanyue Shen Xiaoming Wu Jianbo Wang | 2014 | 2014 (4-5)2014，，4： | 1 |
| 14 | Mesoporous LiFePO 4 microspheres for rechargeable lithium-ion batteries显示文摘 | Juan Du Lifang Jiao Qiong Wu Yongchang Liu Zhan Qi Lijing Guo Yijing Wang Huatang Yuan | 2013 | Electrochimica Acta2013，，： | 1 |
| 15 | Synthesis and characterization of Li 2 FeP 2 O 7 /C nanocomposites as cathode materials for Li-ion batteries显示文摘 | Juan Du Lifang Jiao Qiong Wu Yongchang Liu Yanping Zhao Lijing Guo Yijing Wang Huatang Yuan | 2013 | Electrochimica Acta2013，，： | 1 |
| 16 | Expression and Inheritance of Nine Transgenes in Rice显示文摘 | Liying Wu Somen Nandi Lifang Chen Raymond L. Rodriguez Ning Huang | 2002 | Transgenic Research2002，，5： | 1 |
| 17 | Mutagencidty and polyeyclic aromatic hydrocarbon content of fumes from heated cooking oils produced in Taiwan显示文摘 | CHIANG T A WU Peifen WANG Lifang | 1997 | Mutation Research1997，381，2： | 1 |
| 18 | Proteomic characterization of royal jelly proteins in Chinese (Apis cerana cerana) and European (Apis mellifera) honeybees显示文摘 | Ning Qu Jie Jiang Liangxian Sun Changcheng Lai Lifang Sun Xueji Wu | 2008 | Biochemistry (Moscow)2008，，6： | 1 |
| 19 | Enhanced blue and green upconversion in hydrothermally synthesized hexagonal NaY1-xYb,F4:Ln3+(Ln3+=Er3+ or Tm3+)显示文摘 | Liang Lifang Wu Hao Hu Haili | 2004 | Journal of Alloy and Compounds2004，368，： | 1 |
| 20 | A comparative study on stabilization of available as in highly contaminated hazardous solid waste显示文摘 | Hu Lifang Feng Huajun Wu Yuyong | 2010 | Journal of Hazardous Materials2010，174，13： | 1 |
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