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| 1 | AXL is a candidate receptor for SARS-CoV-2 that promotes infection of pulmonary and bronchial epithelial cells显示文摘The current coronavirus disease 2019(COVID-19)pandemic presents a global public health challenge.The viral pathogen responsible,severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),binds to the host receptor ACE2 through its spike(S)glycoprotein,which mediates membrane fusion and viral entry.Although the role of ACE2 as a receptor for SARS-CoV-2 is clear,studies have shown that ACE2 expression is extremely low in various human tissues,especially in the respiratory tract.Thus,other host receptors and/or co-receptors that promote the entry of SARS-CoV-2 into cells of the respiratory system may exist.In this study,we found that the tyrosine-protein kinase receptor UFO(AXL)specifically interacts with the N-terminal domain of SARS-CoV-2 S.Using both a SARS-CoV-2 virus pseudotype and authentic SARS-CoV-2,we found that overexpression of AXL in HEK293T cells promotes SARS-CoV-2 entry as efficiently as overexpression of ACE2,while knocking out AXL significantly reduces SARS-CoV-2 infection in HI 299 pulmonary cells and in human primary lung epithelial cells.Soluble human recombinant AXL blocks SARS-CoV-2 infection in cells expressing high levels of AXL.The AXL expression level is well correlated with SARS-CoV-2 S level in bronchoalveolar lavage fluid cells from COVID-19 patients.Taken together,our findings suggest that AXL is a novel candidate receptor for SARS-CoV-2 which may play an important role in promoting viral infection of the human respiratory system and indicate that it is a potential target for future clinical intervention strategies. | Shuai Wang Zongyang Qiu Yingnan Hou Xiya Deng Wei Xu Tingting Zheng Peihan Wu Shaofang Xie Weixiang Bian Chong Zhang Zewei Sun Kunpeng Liu Chao Shan Aifu Lin Shibo Jiang Youhua Xie Qiang Zhou Lu Lu Jing Huang Xu Li | 2021 | Cell Research2021，31，2： | 39 |
| 2 | C_(17)-fengycin B,produced by deep-sea-derived Bacillus subtilis,possessing a strong antifungal activity against Fusarium solani显示文摘Root rot disease caused by Fusarium solani is the most devastating disease of the tomato and legume crops in China.The metabolites of Bacillus species can inhibit many fungal diseases.In this study,the metabolites of deep-sea-derived bacterium Bacillus subtilis 2 H11 can significantly inhibit the growth of F.solani.The metabolite C_(17)-fengycin B,one of the cyclic lipopeptides,was identified by the combination of silica column chromatography,high-performance liquid chromatography(HPLC),high-energy collision induced dissociation mass spectrometry(HCD-MS)and tandem mass spectrometry(HCD-MS/MS).The results of scanning electron microscopy(SEM)and transmission electron microscopy(TEM)showed that C_(17)-fengycin B could destroy the structure of the hyphae and spores of F.solani.The antifungal activities of C_(17)-fengycin B against F.solani were tested at concentrations ranging from 0.05 mg/mL to 0.20 mg/mL.The results indicated that C_(17)-fengycin B inhibited the growth of F.solani with antifungal index of 89.80%at 0.20 mg/mL,and the antifungal activity of C_(17)-fengycin B was further verified by the pot experiment.In addition,the cytotoxicity experiment showed that C_(17)-fengycin B had good biocompatibility and was a potential candidate for the development of biocontrol pesticide in the future. | Weixiang LIU Chaomin SUN | 2021 | Journal of Oceanology and Limnology2021，39，5： | 5 |
| 3 | Extraction and back-extraction behaviors of 14 rare earth elements from sulfuric acid medium by TODGA显示文摘The unique physical and chemical properties of rare earth elements lay the foundation for their extensive application. N,N,N',N' Tetra-octyl-3-oxopentanediamide(TODGA) is excellent in its ability of extracting rare earth elements and it is favored for green initiative. In this paper, the extraction and back-extraction of14 rare earth elements by TODGA were studied. Experiments show that in conditions of 6 mol/L sulfuric acid, the extraction temperature of 25 ℃,the phase ratio of 1:1 and 0.04 mol/LTODGA(aviation kerosene as the diluent), the extraction rates of 14 rare earth elements including lanthanum, cerium, praseodymium,neodymium, europium, gadolinium, terbium, dysprosium, holmium, erbium, thulium, ytterbium, lutetium, and yttrium were 99.00%-99.73%. Mixed with hydrochloric acid and nitric acid(HCl 3.5 mol/L, HNO_30.5 mol/L), the recoveries of the 14 rare earth elements are 91.52%-99.91% when the extraction temperature is 25 ℃ and the ratio is 1:1. The following application is based on the optimum conditions above with practical samples(from the roasting production line of China North Rare Earth High-tech Company Limited) for extraction and back-extraction experiments. Experiments show that TODGA has excellent enrichment effect on 14 rare earth elements, the extraction rates are 91.36%-99.80%, the back-extraction rates are 87.29%-99.64% and the total recoveries are 81.19%-99.44%. | Huiting Yuan Weixiang Hong Yushan Zhou Baisong Pu Aijun Gong Tao Xu Qishan Yang Fukai Li Lina Qiu Weiwei Zhang Yuning Liu | 2018 | Journal of Rare Earths2018，36，6： | 4 |
| 4 | Neural-network adaptive controller for nonlinear systems and its application in pneumatic servo systems显示文摘In this paper, a novel control law is presented, which uses neural-network techniques to approximate the affine class nonlinear system having unknown or uncertain dynamics and noise disturbances. It adopts an adaptive control law to adjust the network parameters online and adds another control component according to H-infinity control theory to attenuate the disturbance. This control law is applied to the position tracking control of pneumatic servo systems. Simulation and experimental results show that the tracking precision and convergence speed is obviously superior to the results by using the basic BP-network controller and self-tuning adaptive controller. | Lu LU Fagui LIU Weixiang SHI | 2008 | 控制理论与应用(英文版)2008，6，1： | 2 |
| 5 | Separation of injectable salidroside by column chromatography of macroporous resins for treating myocardial ischemia显示文摘The objective of the present study is to develop a method for large-scale separating and purifying salidroside from rhodiola kirilowii roots and for preparing injectable medicinal ingredient.Crude extract of salidroside was prepared by water-ethanol system,and purified by column chromatography of macroporous resins.Static adsorption and desorption studies were performed on six kinds of macroporous resins,and SP825 resin was chosen,followed by optimizing process parameters.The optimum sample volume,feed concentration,ratio of diameter to height,and feeding flow rate were 1.5 bed volumes(BV),15 mg/mL,1:10 and 1 BV/h,respectively.Dynamic desorption was performed consecutively with 8 BV of distilled water,3 BV of 5% ethanol and 8 BV of 10% ethanol at a flow rate of 2 BV/h.After three cycles in separating 3.5 tons of rhodiola kirilowii roots,salidroside purity was increased from 3.4% in the crude extract to 93.6% in purified salidroside product.This study provides a novel method to separate salidroside for injectable use. | JU RuiJun HUANG RenJie ZHOU Jia LI RuoJing ZHOU Peng ZHANG ZaoHua XIANG FeiJun XU DongJin LIU WeiXiang MA XingTian ZHANG Qiang LU WanLiang | 2012 | Science China Chemistry2012，55，7： | 2 |
| 6 | Hyperbolic polynomial uniform B-spline curves and surfaces with shape parameter显示文摘 | Liu Xumin Xu Weixiang Guan Yong Shang Yuanyuan | 2009 | Graphical Models2009，，1： | 2 |
| 7 | Transgenic Bt rice does not affect enzyme activities and microbial composition in the rhizosphere during crop development显示文摘 | Wei Liu Hao Hao Lu Weixiang Wu Qi Kun Wei Ying Xu Chen Janice E. Thies | 2007 | Soil Biology and Biochemistry2007，，2： | 2 |
| 8 | Hyperbolic polynomial uniform B-spline curves and surfaces with shape parameter显示文摘 | Liu Xumin Xu Weixiang Guan Yong Shang Yuanyuan | 2010 | Graphical Models2010，72，1： | 1 |
| 9 | Physical and Electrochemical Characterizations of Microwave-assisted Polyol Preparation of Carbon-supported PtRu Nanoparticles显示文摘 | Liu Zhaolin Jim Yang Lee Chen Weixiang | 2004 | Langmuir2004，20，： | 1 |
| 10 | On Alpha-Divergence Based N0n-negative Matr Factorization for Clustering Cancer Gene Expression Data显示文摘 | Liu Weixiang Ye Datian Yuan Kehong | 2008 | Artificial Intelligence in Medicine2008，44，1： | 1 |
| 11 | Overexpression of the mTERT gene by adenoviral vectors promotes the proliferation of neuronal stem cells in vitro and stimulates neurogenesis in the hippocampus of mice显示文摘We sought to construct the adenoviral vector carrying the gene encoding mouse telomerase reverse transcriptase(mTERT),as well as detect its expression and effect on the proliferation of neuronal stem cells.mTERT was am-plified by RT-PCR and then the eukaryotic expression vector of pDC-EGFP-TERT was constructed.After DNA sequence analysis,we detected that there were 293 cells transfected with pDC-EGFP-TERT and helper adenovirus plasmid pBHG lox ΔE1,and three Cre using Lipofectamine 2000 mediation,named Ad-mTERT-GFP,to pack-age adenoviral particles.The Ad-mTERT-GFP was used to infect neuronal stem cells and then the expression and activity of mTERT were detected.In addition,Bromodeoxyuridine labeling test identified the impact of mTERT overexpression on proliferation of neuronal stem cells.The recombinant adenoviral vector confirmed that mTERT was successfully constructed.Overexpression of mTERT stimulated the proliferation of neuronal stem cells both in vitro and in vivo.mTERT overexpression via adenoviral vector carrying mTERT cDNA upregulated the ability of proliferation in neuronal stem cells. | Mengying Liu Yao Hu Lijuan Zhu Chen Chen Yu Zhang Weixiang Sun Qigang Zhou | 2012 | The Journal of Biomedical Research2012，26，5： | 1 |
| 12 | Reduction of nitrogen loss and Cu and Zn mobility during sludge composting with bamboo charcoal amendment显示文摘 | Li Hua Weixiang Wu Yuxue Liu Murray B. McBride Yingxu Chen | 2009 | Environmental Science and Pollution Research2009，，1： | 1 |
| 13 | Cyclohexanol dehydrogenation over Co/carbon nanotube catalysts and the effect of promoter K on performance显示文摘 | Zong-Jian Liu Zhude Xu Zhong-Yong Yuan Deyi Lu Weixiang Chen Wuzong Zhou | 2001 | Catalysis Letters (-)2001，，3： | 1 |
| 14 | Pharmacological inhibition of BAP1 recruits HERC2 to competitively dissociate BRCA1-BARD1, suppresses DNA repair and sensitizes CRC to radiotherapy显示文摘Radiotherapy is widely used in the management of advanced colorectal cancer(CRC).However,the clinical efficacy is limited by the safe irradiated dose.Sensitizing tumor cells to radiotherapy via interrupting DNA repair is a promising approach to conquering the limitation.The BRCA1-BARD1 complex has been demonstrated to play a critical role in homologous recombination(HR)DSB repair,and its functions may be affected by HERC2 or BAP1.Accumulated evidence illustrates that the ubiquitination-deubiquitination balance is involved in these processes;however,the precise mechanism for the cross-talk among these proteins in HR repair following radiation hasn’t been defined.Through activity-based profiling,we identified PT33 as an active entity for HR repair suppression.Subsequently,we revealed that BAP1 serves as a novel molecular target of PT33 via a CRISPR-based deubiquitinase screen.Mechanistically,pharmacological covalent inhibition of BAP1 with PT33 recruits HERC2 to compete with BARD1 for BRCA1 interaction,interrupting HR repair.Consequently,PT33 treatment can substantially enhance the sensitivity of CRC cells to radiotherapy in vitro and in vivo.Overall,these findings provide a mechanistic basis for PT33-induced HR suppression and may guide an effective strategy to improve therapeutic gain. | Xin Yue Tingyu Liu Xuecen Wang Weijian Wu Gesi Wen Yang Yi Jiaxin Wu Ziyang Wang Weixiang Zhan Ruirui Wu Yuan Meng Zhirui Cao Liyuan Le Wenyan Qiu Xiaoyue Zhang Zhenyu Li Yong Chen Guohui Wan Xianzhang Bu Zhenwei Peng Ran-yi Liu | 2023 | Acta Pharmaceutica Sinica B2023，13，8： | 1 |
| 15 | Reduction of nitrogen loss and Cu and Zn mobility during sludge composting with bamboo charcoal amendment显示文摘 | Li Hua Weixiang Wu Yuxue Liu Murray B. McBride Yingxu Chen | 2009 | Environmental Science and Pollution Research2009，，1： | 1 |
| 16 | Degradative solvent extraction of low-rank coal:Role of water on pyrolysis mechanism of low-rank coal in a highly-dispersed medium显示文摘A degradative solvent extraction(DSE)method was proposed to upgrade low-rank coals(LRC)for their cascaded utilizations in a highly-dispersed medium.The derived products exhibited well improved properties in comparison to the raw LRCs.Previously,the LRCs were pre-dried to eliminate the potential impact of the varied water content of raw LRCs,but in light of the abundant water in fresh coals,the hydrolyzing effect of water on coals under heating,and the energy cost to pre-dry the LRCs,it is consequently essential to clarify the effect of water so as to verify the necessity of pre-drying process.In this study,the roles of inherent water and extra-added water were respectively investigated,using dried,raw and wet coals from two typical LRCs.The results show that increasing the moisture content of raw LRCs contributed to the extraction ability of DSE method without noticeably changing the elemental composition,chemical structure and thermal decomposition behavior of extractable products,thus pre-drying of LRCs before DSE treatment was proven unnecessary.Additionally,the roles of the inherent water were concluded as:1)leading to the formation of stable covalent bond during drying process;and 2)acting as H donor to promote extraction ability,while the added water can only function as H donor.Since excessive water will increase the pressure during DSE treatment and lead to the waste water treatment,the LRCs with a water content between 10%and 30%were consequently recommended for the practical application of DSE treatment. | Zhenzhong Hu Xu Zhang Xian Li Weixiang Qian Guangqian Luo Huan Liu Hongyun Hu Nakorn Worasuwannarak Hong Yao | 2022 | Carbon Resources Conversion2022，5，2： | 1 |
| 17 | Hyerbolic polynomial uniform B-spline curves and surfaces with shape parameters 显示文摘 | Liu Xumin Xu Weixiang Guan Yong | 2010 | Graphical Models2010，72，1： | 1 |
| 18 | Evaluation of biochar effects on nitrogen retention and leaching in Multi-layered soil columns显示文摘 | Ding Y Liu Yuxue Wu Weixiang | 2010 | Water Air Soil Pollt2010，213，1234： | 1 |
| 19 | Meso-cenozoie taphrogeny and extensional tectonics in eastern China显示文摘 | Xingyuan LIU He{u Wang Weixiang el al | 1983 | Acta Geologica Sinica1983，57，1： | 1 |
| 20 | Epigenetic Regulation of miR-184 by MBD1 Governs Neural Stem Cell Proliferation and Differentiation显示文摘 | Changmei Liu Zhao-Qian Teng Nicholas J. Santistevan Keith E. Szulwach Weixiang Guo Peng Jin Xinyu Zhao | 2010 | Cell Stem Cell2010，，5： | 1 |
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