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| 1 | Developing superior alleles of yield genes in rice by artificial mutagenesis using the CRISPR/Cas9 system显示文摘Rice yield is an important and complex agronomic trait controlled by multiple genes.In recent decades,dozens of yield-associated genes in rice have been cloned,many of which can increase production in the form of loss or degeneration of function.However,mutations occurring randomly under natural conditions have provided very limited genetic resources for yield increases.In this study,potentially yield-increasing alleles of two genes closely associated with yield were edited artificially.The recently developed CRISPR/Cas9system was used to edit two yield genes:Grain number 1a(Gn1a)and DENSE AND ERECT PANICLE1(DEP1).Several mutants were identified by a target sequence analysis.Phenotypic analysis confirmed one mutant allele of Gn1a and three of DEP1 conferring yield superior to that conferred by other natural high-yield alleles.Our results demonstrate that favorable alleles of the Gnla and DEP1 genes,which are considered key factors in rice yield increases,could be developed by artificial mutagenesis using genome editing technology. | Liyu Huang Ru Zhang Guangfu Huang Yanxia Li Getachew Melaku Shilai Zhang Haitao Chen Yanjuan Zhao Jing Zhang Yesheng Zhang Fengyi Hu | 2018 | The Crop Journal2018，6，5： | 8 |
| 2 | Identification of Key Genes for the Ultrahigh Yield of Rice Using Dynamic Cross-tissue Network Analysis显示文摘Significantly increasing crop yield is a major and worldwide challenge for food supply and security.It is well-known that rice cultivated at Taoyuan in Yunnan of China can produce the highest yield worldwide.Yet,the gene regulatory mechanism underpinning this ultrahigh yield has been a mystery.Here,we systematically collected the transcriptome data for seven key tissues at different developmental stages using rice cultivated both at Taoyuan as the case group and at another regular rice planting place Jinghong as the control group.We identified the top 24 candidate high-yield genes with their network modules from these well-designed datasets by developing a novel computational systems biology method,i.e.,dynamic cross-tissue(DCT)network analysis.We used one of the candidate genes,Os SPL4,whose function was previously unknown,for gene editing experimental validation of the high yield,and confirmed that Os SPL4 significantly affects panicle branching and increases the rice yield.This study,which included extensive field phenotyping,cross-tissue systems biology analyses,and functional validation,uncovered the key genes and gene regulatory networks underpinning the ultrahigh yield of rice.The DCT method could be applied to other plant or animal systems if different phenotypes under various environments with the common genome sequences of the examined sample.DCT can be downloaded from https://github.com/ztpub/DCT. | Jihong Hu Tao Zeng Qiongmei Xia Liyu Huang Yesheng Zhang Chuanchao Zhang Yan Zeng Hui Liu Shilai Zhang Guangfu Huang Wenting Wan Yi Ding Fengyi Hu Congdang Yang Luonan Chen Wen Wang | 2020 | Genomics, Proteomics & Bioinformatics2020，18，3： | 2 |
| 3 | Combination therapy of thiamine,vitamin C and hydrocortisone in treating patients with sepsis and septic shock:a meta-analysis and trial sequential analysis显示文摘Background:The objective of this study was to evaluate the clinical efficacy of thiamine and vitamin C with or without hydrocortisone coadministration on the treatment of sepsis and septic shock.Methods:MEDLINE,EMBASE and CENTRAL databases were searched for randomized controlled trials(RCTs)that made a comparative study between the combination therapy of vitamin C and thiamine with or without hydrocortisone and the administration of placebo in patients with sepsis or septic shock.Two reviewers independently performed study selection,data extraction and quality assessment.Both short-term mortality and change in the sequential organ failure assessment(SOFA)score from baseline(delta SOFA)were set as the primary outcomes.Secondary endpoints included intensive care unit(ICU)mortality,new onset of acute kidney injury,total adverse events,ICU and hospital length of stay,duration of vasopressor usage and ventilator-free days.Meanwhile,trial sequential analysis was conducted for primary outcomes.Results:Eight RCTs with 1428 patients were included in the current study.The results showed no significant reduction of short-term mortality in sepsis and septic shock patients who received combination therapy of vitamin C and thiamine with or without hydrocortisone compared to those with placebo{risk ratio(RR),1.02[95%confidence interval(CI),0.87 to 1.20],p=0.81,I2=0%;risk difference(RD),0[95%CI,−0.04 to 0.05]}.Nevertheless,the combination therapy was associated with significant reduction in SOFA score[mean difference(MD),−0.63,(95%CI,−0.96 to−0.29,p<0.001,I^(2)=0%]and vasopressors duration(MD,−22.11[95%CI,−30.46 to−13.77],p<0.001,I2=6%).Additionally,there were no statistical differences in the pooled estimate for other outcomes.Conclusions:In the current meta-analysis,the combination therapy of vitamin C and thiamine,with or without hydrocortisone had no impact on short-term mortality when compared with placebo,but was associated with significant reduction in SOFA score among patients with sepsis and septic shock. | Renqi Yao Yibing Zhu Yue Yu Zhixuan Li Lixue Wang Liyu Zheng Jingyan Li Huibin Huang Guosheng Wu Feng Zhu Zhaofan Xia Chao Ren Yongming Yao | 2021 | Burns & Trauma2021，9，1： | 2 |
| 4 | 2.29-kV GaN-based double-channel Schottky barrier diodes on Si substrates with high VON uniformity显示文摘The GaN on Si technology is attractive for power electronic systems owing to its low cost and large wafer size[1].GaN Schottky barrier diodes(SBDs)on Si substrates have been extensively studied due to their superior power figure of merit and fast reverse recovery features[2]. | Ren HUANG Weihang ZHANG Jincheng ZHANG Chunxu SU Xi LIU Liyu FU Yue HAO | 2023 | Science China(Information Sciences)2023，66，6： | 2 |
| 5 | Genome and Comparative Transcriptomics of African Wild Rice Oryza Iongistaminata Provide Insights into Molecular Mechanism of Rhizomatousness and Self-Incompatibility显示文摘Dear Editor, Oryza Iongistaminata is an African wild rice species with AA genome type possessing special traits that are highly valued for improving cultivated rice,such as strong resistance to biotic and abiotic stresses (Song et al.,1995) for improving resistance of cultivars,rhizomatousness for perennial breeding (Glover et al.,2010),and self-incompatibility (SI) for new ways to produce hybrid seeds (Ghesquiere,1986).Deciphering the genome of O.longistaminata will be the key to uncovering the mechanism of these hallmark traits and improving cultivated rice. | Yesheng Zhang Shilai Zhang Hui Liu Binying Fu Lijuan Li Min Xie Yue Song Xin Li Jing Cai Wenting Wan Ling Kui Hui Huang Jun Lyu Yang Dong Wensheng Wang Liyu Fluang Jing Zhang Qinzhong Yang Qinfi Shan Qiong Li Wangqi Huang Dayun Tao Muhua Wang Mingsheng Chen Yeisoo Yu Rod A. Wing Wen Wang Fengyi Hu | 2015 | Molecular Plant2015，8，11： | 1 |
| 6 | Differentialregulation of hepatic bile salt and organic anion transporters in pregnant and postpartum rat and the role of prolactin显示文摘 | Liyue huang Yang liu | 2001 | Hepatology2001，33，： | 1 |
| 7 | Research on coupled thermo-hydro-mechanical dynamic response characteristics of saturated porous deep-sea sediments under vibration of mining vehicle显示文摘The excessive deformation of deep-sea sediments caused by the vibration of the mining machine will adversely affect the efficiency and safety of mining.Combined with the deep-sea environment,the coupled thermo-hydro-mechanical problem for saturated porous deep-sea sediments subject to the vibration of the mining vehicle is investigated.Based on the Green-Lindsay(G-L)generalized thermoelastic theory and Darcy’s law,the model of thermo-hydro-mechanical dynamic responses for saturated porous deep-sea sediments under the vibration of the mining vehicle is established.We obtain the analytical solutions of non-dimensional vertical displacement,excess pore water pressure,vertical stress,temperature,and change in the volume fraction field with the normal mode analysis method,and depict them graphically.The normal mode analysis method uses the canonical coordinate transformation to solve the equation,which can quickly decouple the equation by ignoring the modal coupling effect on the basis of the canonical mode.The results indicate that the vibration frequency has obvious influence on the vertical displacement,excess pore water pressure,vertical stress,and change in volume fraction field.The loading amplitude has a great effect on the physical quantities in the foundation,and the changes of the physical quantities increase with the increase in loading amplitude. | Wei ZHU Xinyu SHI Rong HUANG Liyue HUANG Wenbo MA | 2021 | Applied Mathematics and Mechanics(English Edition)2021，42，9： | 1 |
| 8 | Chronic bronchitis leads to accelerated hyperinflation in COPD patients during exercise显示文摘 | Wen Zhang Haitao Lu Liyue Peng Xiaoxia Ren Yong Lu Li An Hong Zhang Xiaoyue Tan Xingguo Sun Kewu Huang | 2015 | Respirology2015，，4： | 1 |
| 9 | Altered effective connectivity patterns of the default mode network in Alzheimer’s disease: An fMRI study显示文摘 | Yufang Zhong Liyu Huang Suping Cai Yun Zhang Karen M. von Deneen Aifeng Ren Junchan Ren | 2014 | Neuroscience Letters2014，，： | 1 |
| 10 | Effect of triiodothyronine on the growth and survival of larval striped bass (Morone saxatilis)显示文摘 | Liyue Huang Jennifer L. Specker David A. Bengtson | 1996 | Fish Physiology and Biochemistry1996，，1： | 1 |
| 11 | Early detection of focal cerebral ishcemia using bispectrum parameters of EEG in neural networks显示文摘 | Huang Liyu Ju Fengchi Cheng Jingzhi | 2003 | IEEE EMBS Conference on Neural Engineering2003，，3： | 1 |
| 12 | Vaccination of Goats with 31 kDa and 32 kDa Schistosoma japonicum Antigens by DNA Priming and Protein Boosting显示文摘Two Schistosoma japonicum vaccine candidate antigens Sj 31 and Sj 32, which have shown particular promise to induce protective immunity in mice, were used to immunize goats by using a DNA priming-protein boosting strategy in present work. DNA vaccine formulations of the two antigens (VRSj31 and VRSj32) were produced and injected intramuscularly twice at a 2-week interval and then recombinant proteins (rSj31 and rSj32) together with Freund Complete Adjuvant (FCA) were used to boost the goats. The experiment was repeated in different batche cercariae. A strong anamnestic antibody response was induced after boost. A significant reduction of liver egg counts and miracidial hatching was showed in both experiments. Significant protections against challenge infection were elicited with 31.6% of percentage reduction for worm recovery in the second experiment and 20.9% in the first experiment, respectively. | Lianfei Tang Zhijun Zhou Yuxiao Chen Yonghui Luo Linqian Wang Liyu Chen Fushen Huang Xianfang Zeng Xinyuan Yi | 2007 | Cellular & Molecular Immunology2007，4，2： | 1 |
| 13 | Bispectrum quantification analysis of EEG and artificial neural network may clas- sify ischemic states显示文摘 | Huang Liyu Wang Weirong Singare S | 2006 | Lecture Notes in Computer Science2006，4233，： | 1 |
| 14 | Nuclear magnetic resonance studies on vanadium(IV) electrolyte solutions for vanadium redox flow battery显示文摘 | M. Vijayakumar Sarah D. Burton Cheng Huang Liyu Li Zhenguo Yang Gordon L. Graff Jun Liu Jianzhi Hu Maria Skyllas-Kazacos | 2010 | Journal of Power Sources2010，，22： | 1 |
| 15 | Deep transcriptome sequencing of rhizome and aerial-shoot in Sorghum propinquum显示文摘 | Ting Zhang Xiuqin Zhao Wensheng Wang Liyu Huang Xiaoyue Liu Ying Zong Linghua Zhu Daichang Yang Binying Fu Zhikang Li | 2014 | Plant Molecular Biology2014，，3： | 1 |
| 16 | Propofol-Induced Anesthesia Alters Corticocortical Functional Connectivity in the Human Brain:An EEG Source Space Analysis显示文摘Dear Editor,Anesthetic-induced unconsciousness is accompanied by decreased information exchange among relevant cortical functional modular areas that support normal cognitive function[1].Using the electroencephalogram(EEG)signal,evidence suggest that the feedback and feedforward connectivity among cortical regions may be asymmetrically affected during anesthetic-induced anesthesia[2]. | Xue Zhao Yubo Wang Yun Zhang Haidong Wang Junchan Ren Fei Yan Dawei Song Ruini Du Qiang Wang Liyu Huang | 2021 | Neuroscience Bulletin2021，37，4： | 1 |
| 17 | The Integrative Studies on the Functional A-to-I RNA Editing Events in Human Cancers显示文摘Adenosine-to-inosine(A-to-I)RNA editing,constituting nearly 90%of all RNA editing events in humans,has been reported to contribute to the tumorigenesis in diverse cancers.However,the comprehensive map for functional A-to-I RNA editing events in cancers is still insufficient.To fill this gap,we systematically and intensively analyzed multiple tumorigenic mechanisms of A-to-I RNA editing events in samples across 33 cancer types from The Cancer Genome Atlas.For individual candidate among1,500,000 quantified RNA editing events,we performed diverse types of downstream functional annotations.Finally,we identified 24,236 potentially functional A-to-I RNA editing events,including the cases in APOL1,IGFBP3,GRIA2,BLCAP,and miR-589-3p.These events might play crucial roles in the scenarios of tumorigenesis,due to their tumor-related editing frequencies or probable effects on altered expression profiles,protein functions,splicing patterns,and microRNA regulations of tumor genes.Our functional A-to-I RNA editing events(https://ccsm.uth.edu/CAeditome/)will help better understand the cancer pathology from the A-to-I RNA editing aspect. | Sijia Wu Zhiwei Fan Pora Kim Liyu Huang Xiaobo Zhou | 2023 | Genomics, Proteomics & Bioinformatics2023，21，3： | 0 |
| 18 | GIGANTEA orthologs,E2 members,redundantly determine photoperiodic flowering and yield in soybean显示文摘Soybean(Glycine max L.)is a typical photoperiodsensitive crop,such that photoperiod determines its flowering time,maturity,grain yield,and phenological adaptability.During evolution,the soybean genome has undergone two duplication events,resulting in about 75%of all genes being represented by multiple copies,which is associated with rampant gene redundancy.Among duplicated genes,the important soybean maturity gene E2 has two homologs,E2-Like a(E2La)and E2-Like b(E2Lb),which encode orthologs of Arabidopsis GIGANTEA(GI).Although E2 was cloned a decade ago,we still know very little about its contribution to flowering time and even less about the function of its homologs.Here,we generated single and double mutants in E2,E2La,and E2Lb by genome editing and determined that E2 plays major roles in the regulation of flowering time and yield,with the two E2 homologs depending on E2 function.At high latitude regions,e2 single mutants showed earlier flowering and high grain yield.Remarkably,in terms of genetic relationship,genes from the legume-specific transcription factor family E1 were epistatic to E2.We established that E2 and E2-like proteins form homodimers or heterodimers to regulate the transcription of E1 family genes,with the homodimer exerting a greater function than the heterodimers.In addition,we established that the H3 haplotype of E2 is the ancestral allele and is mainly restricted to low latitude regions,from which the loss-of-function alleles of the H1 and H2haplotypes were derived.Furthermore,we demonstrated that the function of the H3 allele is stronger than that of the H1 haplotype in the regulation of flowering time,which has not been shown before.Our findings provide excellent allelic combinations for classical breeding and targeted gene disruption or editing. | Lingshuang Wang Haiyang Li Milan He Lidong Dong Zerong Huang Liyu Chen Haiyang Nan Fanjiang Kong Baohui Liu Xiaohui Zhao | 2023 | Journal of Integrative Plant Biology2023，65，1： | 0 |
| 19 | Design Method for Mix Proportion of Hydraulic High Performance Concrete Based on Overall Calculation显示文摘 | Jun Feng Shuyou Huang Bingfang Zhao Liyu Sun | 2014 | 土木工程与技术(中英文版)2014，3，2： | 0 |
| 20 | ENERGY FEATURE EXTRACTION AND SVM CLASSIFICATION OFMOTORIMAGERY-INDUCED ELECTROENCEPHALOGRAMS显示文摘The precise classification for the electroencephalogram(EEG)in different mental tasks in the research on braincomputer interface(BCI)is the key for the design and clinical application of the system.In this paper,a new combination classification algorithm is presented and tested using the EEG data of right and left motor imagery experiments.First,to eliminate the low frequency noise in the original EEGs,the signals were decomposed by empirical mode decomposition(EMD)and then the optimal kernel parameters for support vector machine(SVM)were determined,the energy features of thefirst three intrinsic mode functions(IMFs)of every signal were extracted and used as input vectors of the employed SVM.The output of the SVM will be classification result for different mental task EEG signals.The study shows that mean identification rate of the proposed algorithm is 95%,which is much better than the present traditional algorithms. | JIANING ZHENG LIYU HUANG JING ZHAO | 2012 | Journal of Innovative Optical Health Sciences2012，5，2： | 0 |
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