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| 1 | Genomic landscape and genetic manipulation of the black soldier fly Hermetia illucens,a natural waste recycler显示文摘The black soldier fly(BSF),Hermetia illucens(Diptera:Stratiomyidae),is renowned for its bioconversion of organic waste into a sustainable source of animal feed.We report a high-quality genome of 1.1 Gb and a consensus set of 16,770 gene models for this beneficial species.Compared to those of other dipteran species,the BSF genome has undergone a substantial expansion in functional modules related to septic adaptation,including immune system factors,olfactory receptors,and cytochrome P450s.We further profiled midgut transcriptomes and associated microbiomes of BSF larvae fed with representative types of organic waste.We find that the pathways related to digestive system and fighting infection are commonly enriched and that Firmicutes bacteria dominate the microbial community in BSF across all diets.To extend its potential practical applications,we further developed an efficient CRISPR/Cas9-based gene editing approach and implemented this to yield flightless and enhanced feeding capacity phenotypes,both of which could expand BSF production capabilities.Our study provides valuable genomic and technical resources for optimizing BSF lines for industrialization. | Shuai Zhan Gangqi Fang Minmin Cai Zongqing Kou Jun Xu Yanghui Cao Liang Bai Yixiang Zhang Yongmao Jiang Xingyu Luo Jian Xu Xia Xu Longyu Zheng Ziniu Yu Hong Yang Zhijian Zhang Sibao Wang Jeffery K.Tomberlin Jibin Zhang Yongping Huang | 2020 | Cell Research2020，30，1： | 9 |
| 2 | Whole genome re-sequencing reveals evolutionary patterns of sacred lotus (Nelumbo nucifera)显示文摘Sacred lotus(Nelumbo nucifera or lotus) is an important aquatic plant in horticulture and ecosystems. As a foundation for exploring genomic variation and evolution among different germplasms, we re-sequenced 19 individuals from three cultivated temperate lotus subgroups(rhizome,seed and flower lotus), one wild temperate lotus subgroup(wild lotus), one tropical lotus group(Thai lotus) and an outgroup(Nelumbo lutea). Through genetic diversity and polymorphism analysis by non-missing SNP sites widely distributed in the whole genome, we confirmed that wild and Thai lotus exhibited greater differentiation with a higher genomic diversity compared to cultivated lotus. Rhizome lotus had the lowest genomic diversity and a closer relationship to wild lotus, whereas the genomes of seed and flower lotus were admixed. Genes in energy metabolism process and plant immunity evolved rapidly in lotus, reflecting local adaptation.We established that candidate genes in genomic regions with significant differentiation associated with temperate and tropical lotus divergence always exhibited highly divergent expression pattern. Together, this study comprehensive and credible interpretates important patterns of genetic diversity and relationships, gene evolution, and genomic signature from ecotypic differentiation of sacred lotus. | Longyu Huang Mei Yang Ling Li Hui Li Dong Yang Tao Shi Pingfang Yang | 2018 | Journal of Integrative Plant Biology2018，60，1： | 8 |
| 3 | Photoreductive degradation of sulfur hexafluoride in the presence of styrene显示文摘硫 hexafluoride (SF6 ) 在空气作为最强大的温室气体之一被知道。由苯乙烯的 SF6 的减少的相片降级被学习了以达到为硫 hexafluoride 污染开发新奇补习。破坏和 SF6 的移动效率(DRE ) 上的反应条件的效果在这研究被检验。两起始的 styrene-to-SF6 比率和起始的氧集中对 DRE 施加重要影响。SF6 移动效率在 0.2 的起始的 styrene-to-SF6 比率到达最大的价值。DRE 与增加氧集中在 0 ~ 0.09 mol/m3 然后减少的范围上与氧集中增加,这被发现。当水蒸汽被喂进煤气的混合时, DRE 稍微在整个学习时间规模上被提高。X光检查光电子光谱学( XPS )分析,和煤气的层析团,光谱测定( GCMS )和 Fourier 转变红外线的光谱学(英尺红外)分析,证明那是将近居住在煤气的阶段的起始的氟的符号在 SiF4 形式的所有,而,起始的硫在元素的硫形式被扔,在相片降级以后。从有毒的副产品免费,相片降级可以为 SF6 消退面对苯乙烯用作一种有希望的技术。 | HUANG Li GU Dinghong Yang Longyu Xia Lanyan ZHANG Renxi HOU Huiqi | 2008 | Journal of Environmental Sciences2008，20，2： | 5 |
| 4 | Recent diagnostic developments at the 100 kJ-level laser facility in China显示文摘A 100 kJ-level laser facility has been designed to study inertial confinement fusion physics in China.This facility incorporates various diagnostic techniques,including optical,x-ray imaging,x-ray spectrum,and fusion product diagnostics,as well as general diagnostics assistance systems and central control and data acquisition systems.This paper describes recent developments in diagnostics at the facility. | Feng Wang Shaoen Jiang Yongkun Ding Shenye Liu Jiamin Yang Sanwei Li Tianxuan Huang Zhurong Cao Zhenghua Yang Xin Hu Wenyong Miao Jiyan Zhang Zhebin Wang Guohong Yang Rongqing Yi Qi Tang Longyu Kuang Zhichao Li Dong Yang Yulong Li Xiaoshi Peng Kuan Ren Baohan Zhang | 2020 | Matter and Radiation at Extremes2020，5，3： | 2 |
| 5 | Development and application of perfect SSR markers in cotton显示文摘Background:This study aimed to develop a set of perfect simple sequence repeat(SSR)markers with a single copy in the cotton genome,to construct a DNA fingerprint database suitable for authentication of cotton cultivars.We optimized the polymerase chain reaction(PCR)system for multi-platform compatibility and improving detection efficiency.Based on the reference genome of upland cotton and 10×resequencing data of 48 basic cotton germplasm lines,single-copy polymorphic SSR sites were identified and developed as diploidization SSR markers.The SSR markers were detected by denaturing polyacrylamide gel electrophoresis(PAGE)for initial screening,then fluorescence capillary electrophoresis for secondary screening.The final perfect SSR markers were evaluated and verified using 210 lines from different sources among Chinese cotton regional trials.Results:Using bioinformatics techniques,1246 SSR markers were designed from 26626 single-copy SSR loci.Adopting a stepwise(primary and secondary)screening strategy,a set of 60 perfect SSR markers was selected with high amplification efficiency and stability,easy interpretation of peak type,multiple allelic variations,high polymorphism information content(PIC)value,uniform chromosome distribution,and single-copy characteristics.A multiplex PCR system was established with ten SSR markers using capillary electrophoresis detection.Conclusions:A set of perfect SSR markers of cotton was developed and a high-throughput SSR marker detection system was established.This study lays a foundation for large-scale and standardized construction of a cotton DNA fingerprint database for authentication of cotton varieties. | WU Yuzhen HUANG Longyu ZHOU Dayun FU Xiaoqiong LI Chao WEI Shoujun PENG Jun KUANG Meng | 2020 | Journal of Cotton Research2020，3，3： | 2 |
| 6 | Ti2CTx MXene-based all-optical modulator显示文摘MXenes,a new class of 2D transition metal carbides,nitrides,and carbonitrides,have attracted much attention due to their outstanding properties.Here,we report the broadband spatial self-phase modulation of Ti2CTx MXene nanosheets dispersed in deionized water in the visible to near-infrared regime,highlighting the broadband nonlinear optical(NLO)response of Ti2CTx MXene.Using ultrafast pulsed laser excitation,the nonlinear refractive index n2 and the thirdorder nonlinear susceptibility χ^(3)monolayer of Ti2CTx MXene were measured to be^10^−13 m^2/W and ~10^−10 esu,respectively.Leveraging the large optical nonlinearity of Ti2CTx MXene,an all-optical modulator in the visible regime was fabricated based on the spatial cross-phase modulation effect.This work suggests that 2D MXenes are ideal broadband NLO materials with excellent prospects in NLO applications. | Jun Yi Jie Li Shuohan Huang Longyu Hu Lili Miao Chujun Zhao Shuangchun Wen Vadym N.Mochalin Apparao M.Rao | 2020 | InfoMat2020，2，3： | 1 |
| 7 | Shallow sea topography detection using fully Polarimetric Gaofen-3 SAR data based on swell patterns显示文摘Compared to single-polarization synthetic aperture radar(SAR)data,fully polarimetric SAR data can provide more detailed information of the sea surface,which is important for applications such as shallow sea topography detection.The Gaofen-3 satellite provides abundant polarimetric SAR data for ocean research.In this paper,a shallow sea topography detection method was proposed based on fully polarimetric Gaofen-3 SAR data.This method considers swell patterns and only requires SAR data and little prior knowledge of the water depth to detect shallow sea topography.Wave tracking was performed based on preprocessed fully polarimetric SAR data,and the water depth was then calculated considering the wave parameters and the linear dispersion relationships.In this paper,four study areas were selected for experiments,and the experimental results indicated that the polarimetric scattering parameterαhad higher detection accuracy than quad-polarization images.The mean relative errors were 14.52%,10.30%,12.56%,and 12.90%,respectively,in the four study areas.In addition,this paper also analyzed the detection ability of this model for different topographies,and the experiments revealed that the topography could be well recognized when the topography gradient is small,the topography gradient direction is close to the wave propagation direction,and the isobath line is regular. | Longyu Huang Chenqing Fan Junmin Meng Jungang Yang Jie Zhang | 2023 | Acta Oceanologica Sinica2023，42，2： | 0 |
| 8 | Genomic divergence in cotton germplasm related to maturity and heterosis显示文摘Commercial varieties of upland cotton(Gossypium hirsutum)have undergone extensive breeding for agronomic traits,such as fiber quality,disease resistance,and yield.Cotton breeding programs have widely used Chinese upland cotton source germplasm(CUCSG)with excellent agronomic traits.A better understanding of the genetic diversity and genomic characteristics of these accessions could accelerate the identification of desirable alleles.Here,we analyzed 10,522 high-quality singlenucleotide polymorphisms(SNP)with the CottonSNP63 K microarray in 137 cotton accessions(including 12 hybrids of upland cotton).These data were used to investigate the genetic diversity,population structure,and genomic characteristics of each population and the contribution of these loci to heterosis.Three subgroups were identified,in agreement with their knownpedigrees,geographical distributions,and times since introduction.For each group,we identified lineagespecific genomic divergence regions,which potentially harbor key alleles that determine the characteristics of each group,such as early maturity-related loci.Investigation of the distribution of heterozygous loci,among 12 commercial cotton hybrids,revealed a potential role for these regions in heterosis.Our study provides insight into the population structure of upland cotton germplasm.Furthermore,the overlap between lineagespecific regions and heterozygous loci,in the high-yield hybrids,suggests a role for these regions in cotton heterosis. | Shoupu He Gaofei Sun Longyu Huang Daigang Yang Panhong Dai Dayun Zhou Yuzhen Wu Xiongfeng Ma Xiongming Du Shoujun Wei Jun Peng Meng Kuang | 2019 | Journal of Integrative Plant Biology2019，61，8： | 0 |
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