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| 1 | Potential Use of a Poultry Manure Digestate as a Biofertiliser:Evaluation of Soil Properties and Lactuca sativa Growth显示文摘Anaerobic digestion is a process that is widely used for the treatment of organic wastes. The digestate can be used as a soil amendment or crop fertiliser. The aims of our work were to evaluate 1) the physicochemical composition and pathogen content in a digestate from poultry manure, according to international regulations, and 2) the effect of its soil application on the major chemical and biological soil properties and on the growth of Lactuca sativa. The experiment consisted of two groups of pots(with and without crop). Treatments applied to each group were as follows: low and high doses of digestate and inorganic fertiliser, and no application(control)(low dose: 70 kg nitrogen(N) ha^(-1) and 21 kg phosphorus(P) ha^(-1); high dose: 210 kg N ha^(-1) and 63 kg P ha^(-1)). Soil samples were taken 7 and 34 d(harvest) after treatment applications. Heavy metal and pathogen contents in the digestate were below the upper limit values. Despite the high pH and electrical conductivity values of the digestate, both soil parameters presented acceptable values for crop growth. Although there were no initial increases in total inorganic N and available P in soil with digestate application, an increase in the fresh weight of crop was observed with the high dose application. This is probably associated with the slow nutrient release from the digestate during the development of the crop. Changes in the microbial community were temporary and occurred at the initial sampling stage of the experiment. | Natalia MóRTOLA Romina ROMANIUK Vanina COSENTINO Maximiliano EIZA Patricia CARFAGNO Pedro RIZZO Patricia BRES Nicolás RIERA Marcos ROBA Mariano BUTTI Daiana SAINZ Lucrecia BRUTTI | 2019 | Pedosphere2019，29，1： | 2 |
| 2 | S-adenosyl-methionine decreases ethanol-induced apoptosis in primary hepatocyte cultures by a c-Jun N-terminal kinase activity-independent mechanism显示文摘AIM:To determine the role of c-Jun N-terminal kinase(JNK)activity in ethanol-induced apoptosis and themodulation of this signaling cascade by S-Adenosyl-methionine(AdoMet).METHODS:Primary hepatocyte cultures werepretreated with 100 μmol/L SP600125,a selective JNKinhibitor,1 mL/L DMSO or 4 mmol/L AdoMet and thenexposed to 100 mmo/L ethanol.Hepatocyte apoptosiswas determined by the TUNEL and DNA ladder assays.JNK activity and its inhibition by SP600125 and AdoMetwere determined by Western blot analysis of c-junphosphorylation and Bid fragmentation.SP600125 andAdoMet effects on the apoptotic signaling pathway weredetermined by Western blot analysis of cytochrome crelease and pro-caspase 3 fragmentation.The AdoMeteffect on glutathione levels was measured by Ellman'smethod and reactive oxygen species(ROS)generationby cell cytometry.RESULTS:The exposure of hepatocytes to ethanolinduced JNK activation,c-jun phosphorylation,Bidfragmentation,cytochrome c release and pro-caspase 3cleavage;these effects were diminished by SP600125,and caused a significant decrease in ethanol-inducedapoptosis(P<0.05).AdoMet exerted an antioxidanteffect maintaining glutathione levels and decreasing ROSgeneration,without a significant effect on JNK activity,and prevented cytochrome c release and pro-caspase 3cleavage. CONCLUSION:The JNK signaling cascade is a keycomponent of the proapoptotic signaling pathwayinduced by ethanol.JNK activation may be independentfrom ROS generation,since AdoMet which exertedantioxidant properties did not have a significant effect onJNK activity.JNK pathway modulator agents and AdoMetmay be components of promising therapies for alcoholicliver disease(ALD)treatment. | Maria del Pilar Cabrales-Romero Lucrecia Márquez-Rosado Samia FatteI-Fazenda Cristina Trejo-Solis Evelia Arce-Popoca Leticia Alemán-Lazarini Saúl Villa-Trevineo | 2006 | World Journal of Gastroenterology2006，12，12： | 2 |
| 3 | Outcomes of a Contemporary Sample of Patients With Atrial Fibrillation Taking Digoxin: Results From the AFBAR Study显示文摘 | Moisés RODRíGUEZ-MA?ERO Fernando OTERO-RAVI?A Javier GARCíA-SEARA Lucrecia ZUGAZA-GURRUCHAGA José M. RODRíGUEZ-GARCíA Rubén BLANCO-RODRíGUEZ Victorino TURRADO TURRADO José M. FERNáNDEZ-VILLAVERDE Rafael C. VIDAL-PéREZ José R. GONZáLEZ-JUANATEY | 2014 | Revista Espa?ola de Cardiología (English Edition)2014，，： | 1 |
| 4 | Assessing glycaemic variability with continuous glucose monitoring system before and after continuous subcutaneous insulin infusion in people with Type 1 diabetes显示文摘 | Elisa Moya Chimenti Lucrecia Herranz de la Morena Pilar Martín Vaquero Lourdes Sáez-de-ibarra María García Domínguez Luis Felipe Pallardo Sánchez | 2010 | Diabetes Research and Clinical Practice2010，，3： | 1 |
| 5 | Connexin43 phosphorylation in brain, cardiac, endothelial and epithelial tissues显示文摘 | Lucrecia Márquez-Rosado Joell L. Solan Clarence A. Dunn Rachael P. Norris Paul D. Lampe | 2011 | BBA - Biomembranes2011，，： | 1 |
| 6 | Phosphorylation at S365 is a gatekeeper event that changes the structure of Cx43 and prevents down-regulation by PKC 显示文摘 | Joell LS Lucrecia Marquez-Rosado Paul LS | 2007 | J Cell Biol2007，179，6： | 1 |
| 7 | Immunomodulation and Anti-Inflammatory Effects of Garlic Compounds显示文摘 | Rodrigo Arreola Saray Quintero-Fabián Rocío Ivette López-Roa Enrique Octavio Flores-Gutiérrez Juan Pablo Reyes-Grajeda Lucrecia Carrera-Quintanar Daniel Ortu?o-Sahagún Oscar Bottasso | 2015 | Journal of Immunology Research2015，，： | 1 |
| 8 | Influence of Cholecystitis State on Pharmacological Response to Cholecystokinin of Isolated Human Gallbladder with Gallstones显示文摘 | María A. Martínez-Cuesta Lucrecia Moreno Julia Morillas Julio Ponce Juan V. Esplugues | 2003 | Digestive Diseases and Sciences2003，，5： | 1 |
| 9 | A single dose of caffeic acid phenethyl ester prevents initiation in a medium-term rat hepatocarcinogenesis model显示文摘AIM: To study of the effect of caffeic acid phenethyl ester (CAPE) on the initiation period in a medium-term assay of hepatocarcinogenesis. METHODS: Male Wistar rats were subjected to a carcinogenic treatment (CT) and sacrificed at 25th d; altered hepatic foci (AHF) were generated efficiently. To a second group of rats a single 20 mg/kg doses of CAPE was given 12 h before initiation with CT and were sacrificed at 25th d. We evaluated the expression of preneoplastic markers as γ-glutamyltranspeptidase (GGT) and glutathione S-transferase type pi protein (GSTp) by histochemistry, RT-PCR and Western blot analyses, respectively. We measured thiobarbituric acid reactive substances (TBARS) in homogenates of liver and used Unscheduled DNA Synthesis (UDS) assay by incorporation of [3H] thymidine (3HdT) in primary hepatocyte cultures (PHC). RESULTS: At 25th d after CT CAPE reduced the observed increase of GGT+AHF by 84% and liver expression of ggt mRNA by 100%. In case of the GSTp protein, the level was reduced by 90%. As indicative of oxidative stress generated by diethylnitrosamine (DEN) 12 h after its administration, we detected a 68% increase of TBARS. When CAPE was administered before DEN, it completely protected from liver TBARS induction. To have an indication of the sole effect of CAPE on initiation, twocarcinogens were tested in a UDS assay in PHC, we used methyl-n-nitrosoguanidine as a direct carcinogen and DEN, as indirect carcinogen. In this assay, genotoxic damage caused by carcinogens was abolished at 5μM CAPE concentration. CONCLUSION: Our results demonstrated that CAPE possesses anti-genotoxic and antineoplastic capabilities, by an anti-oxidative and free-radical scavenging mechanism. | Claudia Esther Carrasco-Legleu Yesennia Sánchez-Pérez Lucrecia Márquez-Rosado Samia Fattel-Fazenda Evelia Arce-Popoca Sergio Hernández-García Saúl Villa-Trevio | 2006 | World Journal of Gastroenterology2006，12，42： | 0 |
| 10 | Chemical and Biological Characterization from Condafia microphylla Fruits, a Native Species of Patagonia Argentina显示文摘 | Patricia Boeri Lucrecia Pifiuel Sandra Sharry Andrea Tombari Daniel Barrio | 2017 | Journal of Agricultural Science and Technology(B)2017，7，6： | 0 |
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