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| 1 | Cultivation Ages Effect on Soil Physicochemical Properties and Heavy Metal Accumulation in Greenhouse Soils显示文摘The intensive management practices in greenhouse production may alter the soil physicochemical properties and contribute to the accumulation of heavy metals(HMs). To determine the HM concentrations in vegetable soil in relation to soil physicochemical properties and cultivation age, we conducted a soil survey for typical greenhouse soils in Shouguang, China. The results indicated that Cd is a major HM pollutant in the tested soils, as the only HM element exceeding the allowed limit for vegetable soil. The surveyed data was analyzed with regression analysis, correlation analysis and canonical correspondence analysis(CCA). A positive correlation is observed between HM pollution level and cultivation age. CCA results suggest that the HM pollution level and distribution in soil are significantly affected by soil physicochemical properties, which was a function of years of cultivation as revealed by regression analysis. In summary, cultivation age is an important factor to affect soil physicochemical properties(organic matter and inorganic nutrients) as well as HM contamination. | WANG Jun MI Wenkui SONG Peipei XIE Hui ZHU Lusheng WANG Jinhua | 2018 | Chinese Geographical Science2018，28，4： | 5 |
| 2 | Biodegradation of organochlorine pesticide endosulfan by bacterial strain Alcaligenes faecalis JBW4显示文摘The recently discovered endosulfan-degrading bacterial strain Alcaligenes faecalis JBW4 was isolated from activated sludge. This strain is able to use endosulfan as a carbon and energy source. The optimal conditions for the growth of strain JBW4 and for biodegradation by this strain were identified, and the metabolic products of endosulfan degradation were studied in detail. The maximum level of endosulfan biodegradation by strain JBW4 was obtained using broth at an initial pH of 7.0, an incubation temperature of 40°C and an endosulfan concentration of 100 mg/L. The concentration of endosulfan was determined by gas chromatography. Strain JBW4 was able to degrade 87.5% of α-endosulfan and 83.9% of β-endosulfan within 5 days. These degradation rates are much higher than the previously reported bacterial strains. Endosulfan diol and endosulfan lactone were the major metabolites detected by gas chromatography-mass spectrometry; endosulfan sulfate, which is a persistent and toxic metabolite, was not detected. These results suggested that A. faecalis JBW4 degrades endosulfan via a non-oxidative pathway. The biodegradation of endosulfan by A. faecalis is reported for the first time. Additionally, the present study indicates that strain JBW4 may have potential for the biodegradation of endosulfan residues. | Lingfen Kong Shaoyuan Zhu Lusheng Zhu Hui Xie Kunchang Su Tongxiang Yan Jun Wang Jinhua Wang Fenghua Wang Fengxia Sun | 2013 | Journal of Environmental Sciences2013，25，11： | 4 |
| 3 | Arabidopsis Blue Light Receptor Phototropin 1 Undergoes Blue Light-Induced Activation in Membrane Microdomains显示文摘Phototropin (辐透) 调停了发信号由蓝光(BL ) 开始了在在植物在血浆膜(下午) 优化光合的轻俘获起一个关键作用。然而,响应 BL 在 PM 位于辐透活动的规定下面的机制仍然保持大部分不清楚。在这研究,由单个粒子的追踪和 phot1-GFP 蛋白质的逐步的 photobleaching 分析,我们在黑暗 phot1 表明了那 ? 蛋白质留在一个不活跃的状态并且主要作为单体存在。Dimerization 和 phot1-GFP 的散开率响应 BL 以一种剂量依赖者方式增加了。相反, BL 没影响 kinase 不活跃的 phot1 D806N -GFP?but 确实提高了它的 dimerization,建议那 phot1 dimerization 独立于 phosphorylation。F ? rster 回声精力转移荧光一生成像显微镜学 ? 分析表明在 phot1-GFP 和充满甾醇的类脂化合物环境的一个标记之间的相互作用, AtRem1.3-mCherry,与 BL 处理的增加的时间被提高。然而,这个 BL 依赖的相互作用不在植物共同表示是明显的 phot1 D806N -GFP 和 AtRem1.3-mCherry,显示那 BL 便于功能的 phot1-GFP 的 translocation 进标记 AtRem1.3 的 microdomains 激活调停辐透的发信号。相反地,甾醇弄空稀释了 phot1-GFP 动力学, dimerization,和 phosphorylation。一起拿,这些结果显示膜 microdomains 充当在 PM 组织为激活的 phot1 的合适的功能必要的平台。 | Yiqun Xue Jingjing Xing Yinglang Wan Xueqin Lv Lusheng Fan Yongdeng Zhang Kai Song Li Wang Xiaohua Wang Xin Deng Frantisek Baluska John M. Christie Jinxing Lin | 2018 | Molecular Plant2018，11，6： | 4 |
| 4 | Spatial distribution of IL4 controls iNKT cell-DC crosstalk in tumors显示文摘The spatiotemporal distribution of cytokines orchestrates immune responses in vivo,yet the underlying mechanisms remain to be explored.We showed here that the spatial distribution of interleukin-4(IL4)in invariant natural killer T(iNKT)cells regulated crosstalk between iNKT cells and dendritic cells(DCs)and controlled iNKT cell-mediated T-helper type 1(Th1)responses.The persistent polarization of IL4 induced by strong lipid antigens,that is,α-galactosylceramide(αGC),caused IL4 accumulation at the immunological synapse(IS),which promoted the activation of the IL4R-STAT6(signal transducer and activator of transcription 6)pathway and production of IL12 in DCs,which enhanced interferon-γ(IFNγ)production in iNKT cells.Conversely,the nonpolarized secretion of IL4 induced by Th2 lipid antigens with a short or unsaturated chain was incapable of enhancing this iNKT cell-DC crosstalk and thus shifted the immune response to a Th2-type response.The nonpolarized secretion of IL4 in response to Th2 lipid antigens was caused by the degradation of Cdc42 in iNKT cells.Moreover,reduced Cdc42 expression was observed in tumorinfiltrating iNKT cells,which impaired IL4 polarization and disturbed iNKT cell-DC crosstalk in tumors. | Lu Wang Zhilan Liu Lili Wang Qielan Wu Xiang Li Di Xie Huimin Zhang Yongdeng Zhang Lusheng Gu Yanhong Xue Ting Yue Gang Liu Wei Ji Haiming Wei Tao Xu Li Bai | 2020 | Cellular & Molecular Immunology2020，17，5： | 4 |
| 5 | Impacts of Packet Collisions on Link Throughput in CSMA Wireless Networks显示文摘It is known that packet collisions in wireless networks will deteriorate system performance, hence substantial efforts have been made to avoid collision in multi-user access designs. Also, there have been many studies on throughput analysis of CSMA wireless networks. However, for a typical CSMA network in which not all nodes can sense each other, it is still not well investigated how link throughputs are affected by collisions. We note that in practical 802.11-like networks, the time is divided into mini-timeslots and packet collisions are in fact unavoidable. Thus, it is desirable to move forward to explore how collisions in such a network will affect system performance. Based on the collision-free ideal CSMA network(ICN) model, this paper attempts to analyze link throughputs when taking the backoff collisions into account and examine the effect of collisions on link throughputs. Specifically, we propose an Extended Ideal CSMA Network(EICN) model to characterize the collision effects as well as the interactions and dependency among links in the network. Based on EICN, we could directly compute link throughputs and collision probabilities. Simulations show that the EICN model is of high accuracy. Under various network topologies and protocol parameter settings, the computation error of link throughputs using EICN is kept to 4% or below. Interestingly, we find that unlike expected, the effect of collisions on link throughputs in a modest CSMA wireless network is not significant, which enriches our understanding on practical CSMA wireless networks such as Wi-Fi. | Caihong Kai Shengli Zhang Lusheng Wang | 2018 | China Communications2018，15，3： | 3 |
| 6 | The Genetic Mechanism of Inertinite in the Middle Jurassic Inertinite- Rich Coal Seams of the Southern Ordos Basin显示文摘also an important geological information carrier of coal forming environments.In the southern section of the Ordos Basin,the No.4 inertinite-rich coal seam of the Middle Jurassic Yan’an Formation in the Binchang Coal field was selected as an example to study the genetic mechanism of the inertinite.In this study,the results obtained from experimental tests of coal rock,including principal and trace elements,stable carbon isotopes,scanning electron microscopy,inertinite reflectance,sporopollen and free radical retorting methods,were analyzed.Then,the findings were combined with the previous understanding of the oxygen content in the atmosphere and ground fire characteristics,in order to discuss the genesis mechanism of inertinite in the No.4 coal seam.The obtained research results were as follows:(1)During the coal forming period of the No.4 coal seam,the overall climate had been relatively dry.There were four relatively dry-wet climate cycles in the No.4 coal seam,which were controlled by the eccentricity astronomical period.The inertinite content were relatively high during the dry periods;(2)The temperature range suitable for microorganism activities during the oxidation processes was between 0 and 80℃.The simulation results of the free radical concentrations showed that the maximum temperature of fusain in the No.4 coal seam during the process of coalification had not exceeded 300℃,which was significantly higher than the temperature range of microorganism activities.Therefore,these were not conducive to the activities of microorganism and formation of inertinite during the coal-forming period;(3)The genesis temperature of the inertinite in the No.4 coal seam was calculated according to the reflectance of the inertinite,which was lower than 400℃.This result supported the cause of wildfire of the inertinite and reflected that the type of wildfire was mainly ground fire,along with partially surface fire.Moreover,the paleogeographic location,climatic conditions,atmospheric oxygen concentration,etc.of the study area showed that the conditions for wildfire events were in fact available;(4)There were dense and scattered fusinite observed in the No.4 coal seam,and the thickness of cell walls were found to differ.It was speculated that this was related to the type of wildfire,combustion temperatures,combustion timeframes,and different initial conditions of the burned objects during the coal forming periods. | Dongdong Wang Qiang Mao Guoqi Dong Shipeng Yang Dawei Lv Lusheng Yin | 2019 | Journal of Geological Research2019，1，3： | 3 |
| 7 | Diamond/β-SiC film as adhesion-enhanced interlayer for top diamond coatings on cemented tungsten carbide substrate显示文摘In present work, diamond/β-SiC composite interlayers were deposited on cemented tungsten carbide(WC-6%Co) substrates by microwave plasma enhanced chemical vapor deposition(MPCVD) using H_2,CH_4 and tetramethylsilane(TMS) gas mixtures. The microstructure, chemical bonding, element distribution and crystalline quality of the composite interlayers were systematically characterized by means of field-emission scanning electron microscopy(FE-SEM), X-ray diffraction(XRD), X-ray photoelectron spectrometer(XPS), electron probe microanalysis(EPMA), Raman spectroscopy and transmission electron microscropy(TEM). The influences of varying TMS flow rates on the diamond/β-SiC composite interlayers were investigated. Through changing the TMS flow rates in the reaction gas, the volume fraction of β-SiC in the composite interlayers were tunable in the range of 12.0%–68.1%. XPS and EPMA analysis reveal that the composite interlayers are composed of C, Si element with little cobalt distribution. The better crystallinity of the diamond in the composite is characterized based on the Raman spectroscopy, which are helpful to deposit top diamond coatings with high quality. Then, the adhesion of top diamond coatings were estimated using Rockwell C indentation analysis, revealing that the adhesion of top diamond coatings on the WC-6%Co substrates can be improved by the interlayers with the diamond/β-SiC composite structures. Comprehensive TEM interfacial analysis exhibits that the cobalt diffusion is weak from WC-6%Co substrate to the composite interlayer. The homogeneous microcrystalline diamond coatings with the most excellent adhesion can be fabricated on the substrates with the composite interlayer with the β-SiC/diamond ratio of about 45%. The composite structures are appropriate for the application in high-efficiency mechanical tool as a buffer layer for the deposition of the diamond coating. | Qingquan Tian Nan Huang Bing Yang Hao Zhuang Chun Wang Zhaofeng Zhai Junhao Li Xinyi Jia Lusheng Liu Xin Jiang | 2017 | Journal of Materials Science & Technology2017，33，10： | 3 |
| 8 | Immobilization of an enzyme from a Fusarium fungus WZ-I for chlorpyrifos degradation显示文摘The free enzyme extracted from WZ-I,which was identified as Fusarium LK.ex Fx,could effectively degrade chlorpyrifos,an organophosphate insecticide.The methods of immobilizing this free enzyme and determined its degradation-related characteristics were investigated.The properties of the immobilized enzyme were compared with those of the free enzyme.The optimal immobilization of the enzyme was achieved in a solution of 30 g/L sodium alginate at 4°C for 4-12 hr.The immobilized enzyme showed the maximal activity at pH 8.0,45°C.The maximum initial rate and the substrate concentration of the immobilized enzyme were less than that of the free enzyme.The immobilized enzyme,therefore,had a higher capacity to withstand a broader range of temperatures and pH conditions than the free enzyme.With varying pH and temperatures,the immobilized enzyme was more active than the free enzyme in the degradation reaction.In addition,the immobilized enzyme exhibited only a slight loss in its initial activity,even after three repeated uses.The results showed that the immobilized enzyme was more resistant to different environmental conditions,suggesting that it was viable for future practical use. | Hui Xie,Lusheng Zhu,Tingting Ma,Jun Wang,Jinhua Wang,Jun Su,Bo Shao College of Resources and Environment,Shandong Agricultural University,Taian 271018,China | 2010 | Journal of Environmental Sciences2010，22，12： | 3 |
| 9 | The ex vivo Microenviroments in MLTC of Poorly Immunogenic Tumor Cells Facilitate Polarization of CD4^+CD25^+ Regulatory T Cells显示文摘在维持平衡外部有免疫力的 system.Recent 的一个重要角色学习的 CD4+CD25+ 规章的 T (TR ) 房间玩证明了 TR 房间可以也在压制反肿瘤免疫者反应起一个关键作用。以便调查 TR 极化上的肿瘤免疫者微型环境和它的影响,糟糕产生免疫性的肿瘤房间线 D5 ( C57BL/6 , H-2b ),产生免疫性的肿瘤房间线 FBL3 ( C57BL/6 , H-2b )并且 H22 BALB/c , H-2d )被用来建立 syngeneic/allogeneic ,糟糕产生免疫性/产生免疫性的混合淋巴细胞肿瘤房间文化( MLTC )。我们的结果表明在告知的 splenocytes 与 D5 肿瘤房间刺激了的 syngeneic 的 MLTC 的CD4+CD25+ T 房间的比例与 H22 房间比那高(0.43%对0.044%,并且类似的结果在与 .The splenocytes 从更高表明的房间比从 D5 肿瘤的 allogeneic MLTC ,房间,和 splenocytes 刺激了机智的CD4+CD25+房间分配的 D5 肿瘤的 syngeneic MLTC 与上层清液刺激了的 D5 肿瘤房间(0.39%对0.04%)刺激的 allogeneic splenocytes 出现了TGF-1 和 Th2 面向的 cytokines (IL-4 和 IL-10 ) 在糟糕产生免疫性的肿瘤房间的 syngeneic MLTC 的上层清液被统治。我们的结果为学习位于肿瘤免疫者监视下面的机制以及为肿瘤免疫疗法提供了有用信息。 | Yan Wang Le Zhou Hongyan Wang Juxiang Xiao Lusheng Si Yili Wang | 2006 | Cellular & Molecular Immunology2006，3，2： | 2 |
| 10 | Effects of 1-octyl-3-methylimidazolium nitrate on the microbes in brown soil显示文摘The toxicity of ionic liquids(ILs) on soil organisms has aroused wide attention due to their high-solubility. The present investigation focused on the toxicity of 1-octyl-3-methylimidazolium nitrate([C_8mim]NO_3) on the microbial populations(bacteria, fungi, and actinomycetes), soil enzyme(urease, dehydrogenase, acid phosphatase, and β-glucosidase)activities, microbial community diversity using terminal restriction fragment length polymorphism(T-RFLP), and abundance of the ammonia monooxygenase(amoA) genes of ammonia-oxidizing archaea(AOA) and ammonia-oxidizing bacteria(AOB) using quantitative real-time polymerase chain reaction(q-PCR) in brown soil at each trial with doses of 0, 1.0, 5.0,and 10.0 mg/kg on days 10, 20, 30, and 40. The contents of [C_8mim]NO_3 in soil were measured using high performance liquid chromatography with recoveries of 84.3% to 85.2%, and changed less than 10% during the experimental period. A significant decrease was observed from the bacteria, fungi and actinomycetes populations at 10.0 mg/kg, at which the urease activity was inhibited and the β-glucosidase activity was stimulated on days 20, 30, and 40. In addition, [C_8mim]NO_3 inhibited the dehydrogenase activity at 10 mg/kg on days 30 and 40 and the acid phosphatase activity on day 20. The diversity of the soil microbial community and the gene abundance of AOA-and AOB-amo A were also inhibited. Furthermore, the present investigation provided more scientific information for the toxicity evaluation of ILs in soil. | Cheng Zhang Jun Wang Lusheng Zhu Zhongkun Du Jinhua Wang Xi Sun Tongtong Zhou | 2018 | Journal of Environmental Sciences2018，30，5： | 2 |
| 11 | Approximations for a Bottleneck Steiner Tree Problem 显示文摘 | Lusheng Wang Ding- Zhu Du | 2002 | Algorithmica2002，32，4： | 1 |
| 12 | Mathematical Modeling for Network Selection in Heterogeneous Wireless Networks--A Tutorial显示文摘 | WANG Lusheng KUO G S | 2013 | IEEE Communications Surveys and Tutorials2013，15，1： | 1 |
| 13 | Removable edges in a cycles of a 4-connected graph显示文摘 | Wu Jichang Li Xueliang Wang Lusheng | 2004 | Discrete Math2004，287，： | 1 |
| 14 | Approximate sequencing for variable length tasks显示文摘 | Mao-Cheng Cai Xiaotie Deng Lusheng Wang | 2003 | Theoretical Computer Science2003，290，3： | 1 |
| 15 | k-Resonance of Open-ended Carbon Nanotubes 显示文摘 | Zhang Fuji Wang Lusheng | 2004 | Journal of Mathematical Chemistry2004，35，： | 1 |
| 16 | Alignment of trees-an alternative to tree edit 显示文摘 | Jiang Tao Wang Lusheng Zhang Kaizhong | 1995 | Theoretical Computer Science1995，143，： | 1 |
| 17 | Development of prophylactic recombinant HPV58-attenuated Shigella live vector vaccine and evaluation of its protective efficacy and immunogenicity in the guinea pig keratoconjunctivitis model显示文摘开发预防 recombinant 稀释 HPV58L1 的 Shigella 实时向量疫苗并且在畿尼猪 keratoconjunctivitis 模型,评估它的保护的功效和 immunogenicity HPV58L1 基因被克隆进向量 pUCmt,然后 subcloned 进自杀向量 pCVD442。recombinant plasmid pCVD442-HPV58L1 被过滤器交配与助手 plasmid PRK2013 介绍进稀释 Shigella (sf301:virG ) 。积极殖民地被聚合酶链反应收获并且证实。有 60 kDa 的分子的重量的 HPV58L1 蛋白质的表示被西方的污点证实。感兴趣的蛋白质到的能力进像病毒的粒子自我装配被传播电子显微镜,和鼠科的红血球红血球凝聚试金识别。畿尼猪 keratoconjunctivitis 模型被用来评估疫苗的保护的功效和 immunogenicity。动物实验证明没有 keratoconjunctivitis 在使免疫的组(稀释 HPV58 的 Shigella ) 发生了,并且 anti-HPV58L1-IgG 和 -IgA 的浆液层次显然被增加 P < 0.05 ) ,但是 anti-sf301 LPS-IgG 稍微就增加了(P > 0.05 ) 。连接酶的 immunosorbent 点试金证明 HPV58L1 特定的 IgA-antibody-secreting 房间(ASC ) 和怒气和淋巴节点的 IgG-ASC 显然也被增加(P < 0.01 ) 。在这研究, recombinant 稀释 HPV58L1 的 Shigella 实时向量疫苗成功地被构造,并且它能在使免疫的动物导致强壮的体液的有免疫力的回答,并且导致保护的抗体生产。 | Wensheng Li Hongli Liu Xiaofeng Yang Jin Zheng Yili Wang Lusheng Si | 2009 | Acta Biochimica et Biophysica Sinica2009，41，2： | 1 |
| 18 | Re- lay sensor placement in wireless sensor networks 显示文摘 | Cheng Xiuzhen Du Dingzhu Wang Lusheng | 2007 | WirelessNetworks2007，14，3： | 1 |
| 19 | An approximation algorithm fora bottleneck k-Steiner tree plane显示文摘 | Wang Lusheng Li Zimao | 2002 | Information Processing problem in the Euclidean Letters2002，81，3： | 1 |
| 20 | An O(n^2) algorithm for signed translocation 显示文摘 | Wang Lusheng Zhu Darning Liu Xiaowen | 2005 | Journal of Computer and System Sciences2005，70，3： | 1 |
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