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| 1 | A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants显示文摘CRISPR/Cas9 染色体指向系统被用于许多种类。然而,为植物报导的 mostCRISPR/Cas9 系统能仅仅修改一个或一些指向地点。这里,我们报导一个柔韧的 CRISPR/Cas9 向量系统,利用植物 codon 优化了 Cas9 基因,为方便、高效率的复合染色体在 monocot 和 dicot 植物编辑。我们设计了基于 PCR 的过程很快产生多重 sgRNA 表达式盒子,它能在一个轮由金门结扎或吉布森汇编克隆被装配进二进制 CRISPR/Cas9 向量。与这个系统,我们与 85.4% 变化评估的一般水准在米饭编辑了 46 个目标地点,主要在 biallelic 和同型结合的地位。我们推想在米饭的大约 16% 同型结合的变化通过相应基于再结合的修理跟随的非相应的加入结束的机制被产生。我们也获得了一致 biallelic,在 Arabidopsis T 1 植物的异质接合、同型结合、妄想的变化。在米饭和 Arabidopsis 的指向的变化是可继承的。我们在 T 0 米饭和 T 1 由同时的指向的 Arabidopsis 植物多重(多达八) 一个基因家庭,在一条 biosynthetic 小径的多重基因,或在单个基因的多重地点的成员。这个系统为为基本研究和基因改进在植物学习多重基因和基因家庭的功能提供了一个万用的工具箱。 | Xingliang Ma Qunyu Zhang Qinlong Zhu Wei Liu Yan Chen Rong Qiu Bin Wang Zhongfang Yang Heying Li Yuru Lin Yongyao Xie Rongxin Shen Shuifu Chen Zhi Wang Yuanling Chen Jingxin Guo Letian Chen Xiucai Zhao Zhicheng Dong Yao-Guang Liu | 2015 | Molecular Plant2015，8，8： | 272 |
| 2 | Interspecific Hybrid Sterility in Rice Is Mediated by OgTPR 1 at the S 1 Locus Encoding a Peptidase- like Protein显示文摘 | Yongyao Xie Peng Xu Jianle Huang Shengjian Ma Xianrong Xie Dayun Tao Letian Chen Yao-Guang Liu | 2017 | Molecular Plant2017，10，8： | 22 |
| 3 | An effective strategy to establish a male sterility mutant mini-library by CRISPR/Cas9-mediated knockout of anther-specific genes in rice显示文摘Male sterility(MS),characterized by functional defects in male organs or gametes,is an important agronomic trait for hybrid seed production,especially for self-pollinated crops such as rice(Oryza sativa L)(Chen and Liu,2014).Spontaneous MS mutants are rare and difficult to maintain in nature,thus limiting basicresearch and breeding.Artificial mutants are typically generated by physical,chemical,or biological mutagenesis(Wei et al,2013).Recently developed genome editing systems such as CRISPR/Cas9 allow efficient and timesaving knockout of endogenous genes at specific sites(Smith et al,2000;Moscou and Bogdanove,2009;Gasiunas et al.,2012). | Kun Ma Jingluan Han Yu Hao Zhongfang Yang Junyu Chen Yao-Guang Liu Qinlong Zhu Letian Chen | 2019 | Journal of Genetics and Genomics2019，46，5： | 6 |
| 4 | OsEDM2L mediates m^(6)A of EAT1 transcript for proper alternative splicing and polyadenylation regulating rice tapetal degradation显示文摘N^(6)-methyladenosine(m^(6)A) modification affects the post-transcriptional regulation of eukaryotic gene expression, but the underlying mechanisms and their effects in plants remain largely unknown. Here,we report that the N^(6)-adenine methyltransferase-like domain-containing protein ENHANCED DOWNY MILDEW 2-LIKE(OsEDM2 L) is essential for rice(Oryza sativa L.) anther development. The osedm2 l knockout mutant showed delayed tapetal programmed cell death(PCD) and defective pollen development. OsEDM2 L interacts with the transcription factors basic helix-loop-helix 142 and TAPETUMDEGENERATIONRETARDATIONto regulate the expression of ETERNAL TAPETUM 1(EAT1), a positive regulator of tapetal PCD. Mutation of OsEDM2 L altered the transcriptomic m^(6)A landscape, and caused a distinct m^(6)A modification of the EAT1 transcript leading to dysregulation of its alternative splicing and polyadenylation, followed by suppression of the EAT1 target genes OsAP25 and OsAP37 for tapetal PCD. Therefore, OsEDM2 L is indispensable for proper messenger RNA m^(6)A modification in rice anther development. | Kun Ma Jingluan Han Zixu Zhang Heying Li Yanchang Zhao Qinlong Zhu Yongyao Xie Yao‐Guang Liu Letian Chen | 2021 | Journal of Integrative Plant Biology2021，63，11： | 1 |
| 5 | Genome‑wide DNA methylation and transcriptome analyses reveal the key gene for wool type variation in sheep显示文摘Background Wool fibers are valuable materials for textile industry.Typical wool fibers are divided into medullated and non-medullated types,with the former generated from primary wool follicles and the latter by either primary or secondary wool follicles.The medullated wool is a common wool type in the ancestors of fine wool sheep before breeding.The fine wool sheep have a non-medullated coat.However,the critical period determining the type of wool follicles is the embryonic stage,which limits the phenotypic observation and variant contrast,making both selection and studies of wool type variation fairly difficult.Results During the breeding of a modern fine(MF)wool sheep population with multiple-ovulation and embryo transfer technique,we serendipitously discovered lambs with ancestral-like coarse(ALC)wool.Whole-genome rese-quencing confirmed ALC wool lambs as a variant type from the MF wool population.We mapped the significantly associated methylation locus on chromosome 4 by using whole genome bisulfite sequencing signals,and in turn identified the SOSTDC1 gene as exons hypermethylated in ALC wool lambs compare to their half/full sibling MF wool lambs.Transcriptome sequencing found that SOSTDC1 was expressed dozens of times more in ALC wool lamb skin than that of MF and was at the top of all differentially expressed genes.An analogy with the transcriptome of coarse/fine wool breeds revealed that differentially expressed genes and enriched pathways at postnatal lamb stage in ALC/MF were highly similar to those at the embryonic stage in the former.Further experiments validated that the SOSTDC1 gene was specifically highly expressed in the nucleus of the dermal papilla of primary wool follicles.Conclusion In this study,we conducted genome-wide differential methylation site association analysis on differen-tial wool type trait,and located the only CpG locus that strongly associated with primary wool follicle development.Combined with transcriptome analysis,SOSTDC1 was identified as the only gene at this locus that was specifically overexpressed in the primary wool follicle stem cells of ALC wool lamb skin.The discovery of this key gene and its epigenetic regulation contributes to understanding the domestication and breeding of fine wool sheep. | Jiankui Wang Guoying Hua Ganxian Cai Yuhao Ma Xue Yang Letian Zhang Rui Li Jianbin Liu Qing Ma Keliang Wu Yaofeng Zhao Xuemei Deng | 2023 | Journal of Animal Science and Biotechnology2023，14，6： | 0 |
| 6 | Progress and Challenges Toward Effective Flexible Perovskite Solar Cells显示文摘The demand for building-integrated photovoltaics and portable energy systems based on flexible photovoltaic technology such as perovskite embedded with exceptional flexibility and a superior power-to-mass ratio is enormous.The photoactive layer,i.e.,the perovskite thin film,as a critical component of flexible perovskite solar cells(F-PSCs),still faces long-term stability issues when deformation occurs due to encountering temperature changes that also affect intrinsic rigidity.This literature investigation summarizes the main factors responsible for the rapid destruction of F-PSCs.We focus on long-term mechanical stability of F-PSCs together with the recent research protocols for improving this performance.Furthermore,we specify the progress in F-PSCs concerning precise design strategies of the functional layer to enhance the flexural endurance of perovskite films,such as internal stress engineering,grain boundary modification,self-healing strategy,and crystallization regulation.The existing challenges of oxygen-moisture stability and advanced encapsulation technologies of F-PSCs are also discussed.As concluding remarks,we propose our viewpoints on the large-scale commercial application of F-PSCs. | Xiongjie Li Haixuan Yu Zhirong Liu Junyi Huang Xiaoting Ma Yuping Liu Qiang Sun Letian Dai Shahzada Ahmad Yan Shen Mingkui Wang | 2023 | Nano-Micro Letters2023，15，11： | 0 |
| 7 | Highly Efficient White Organic Light-emitting Devices with Optimized Electron Transporting Layers显示文摘We fabricated three phosphorescent blue organic light-emitting devices based on blue phosphor of iri-dium(Ⅲ) bis[(4,6-difluorophenyl)-pyridinato-N,C2'](Firpic) with different electron transporting layer(ETL) materialsBy analyzing efficiency curves and spectral characteristics, the significant effect of ETL on many aspects of deviceperformance was demonstrated. With optimized ETL, the characteristics of devices, such as voltage and efficiencywere significantly improved. Combined with a yellow phosphor of iridium(Ⅲ) bis(4-phenylthieno[3,2-c]pyridinato-N,C2') acetylacetonate(PO-01), phosphorescent white organic light-emitting devices(PhWOLEDs) were obtained.Then, with an aim to promote the performance of the PhWOLEDs~ a thin layer of 4,4',4"-tri(N-carbazolyl)-triphenylamine(TCTA) was introduced between two light emission layers, and the diffusion of excitons was confined.The outperformance device fabricated with 4,7-diphenyl-l,10-phenanthroline(Bphen) as the electron transportinglayer exhibited a peak current efficiency of 36.6 cd/A, a peak power efficiency of 19.2 lm/W, and the InternationalCommission on Illumination coordinates(0.37, 0.46). | ZHANG Tianyu ZHENG Yu WANG Chengming ZHANG Yixin LIU Shihao MA Jian ZHANG Letian XIE Wenfa CHEN Ping LIN Jun LIU Yujuan | 2017 | Chemical Research in Chinese Universities2017，33，2： | 0 |
| 8 | Unraveling the Effect of Stacking Configurations on Charge Transfer in WS_(2) and Organic Semiconductor Heterojunctions显示文摘Photoinduced interfacial charge transfer plays a critical role in energy conversion involving van der Waals(vdW)heterostructures constructed of inorganic nanostructures and organic materials.However,the effect of molecular stacking configurations on charge transfer dynamics is less understood.In this study,we demonstrated the tunability of interfacial charge separation in a type-Ⅱ heterojunction between monolayer(ML)WS_(2) and an organic semiconducting molecule[2-(3″′,4′-dimethyl-[2,2′:5′,2′:5″,2″′-quaterthiophen]-5-yl)ethan-1-ammonium halide(4Tm)]by rational design of relative stacking configurations.The assembly between ML-WS_(2) and the 4Tm molecule forms a face-to-face stacking when 4Tm molecules are in a selfaggregation state.In contrast,a face-to-edge stacking is observed when 4Tm molecule is incorporated into a 2D organic-inorganic hybrid perovskite lattice.The face-to-face stacking was proved to be more favorable for hole transfer from WS_(2) to 4Tm and led to interlayer excitons(IEs)emission.Transient absorption measurements show that the hole transfer occurs on a time scale of 150 fs.On the other hand,the face-to-edge stacking resulted in much slower hole transfer without formation of IEs.This inefficient hole transfer occurs on a similar time scale as A exciton recombination in WS_(2),leading to the formation of negative trions.These investigations offer important fundamental insights into the charge transfer processes at organic−inorganic interfaces. | Shuchen Zhang Dewei Sun Jiaonan Sun Ke Ma Zitang Wei Jee Yung Park Aidan H.Coffey Chenhui Zhu Letian Dou Libai Huang | 2023 | Precision Chemistry2023，1，7： | 0 |
| 9 | FFAR4 improves the senescence of tubular epithelial cells by AMPK/SirT3 signaling in acute kidney injury显示文摘Acute kidney injury(AKI)is a serious clinical complication with high morbidity and mortality rates.Despite substantial progress in understanding the mechanism of AKI,no effective therapy is available for treatment or prevention.We previously found that G protein-coupled receptor(GPCR)family member free fatty acid receptor 4(FFAR4)agonist TUG891 alleviated kidney dysfunction and tubular injury in AKI mice.However,the versatile role of FFAR4 in kidney has not been well characterized.In the study,the expression of FFAR4 was abnormally decreased in tubular epithelial cells(TECs)of cisplatin,cecal ligation/perforation and ischemia/reperfusion injury-induced AKI mice,respectively.Systemic and conditional TEC-specific knockout of FFAR4 aggravated renal function and pathological damage,whereas FFAR4 activation by TUG-891 alleviated the severity of disease in cisplatin-induced AKI mice.Notably,FFAR4,as a key determinant,was firstly explored to regulate cellular senescence both in injured kidneys of AKI mice and TECs,which was indicated by senescence-associatedβ-galactosidase(SA-β-gal)activity,marker protein p53,p21,Lamin B1,phospho-histone H2A.X,phospho-Rb expression,and secretory phenotype IL-6 level.Mechanistically,pharmacological activation and overexpression of FFAR4 reversed the decrease of aging-related SirT3 protein,where FFAR4 regulated SirT3 expression to exhibit anti-senescent effect via Gq subunit-mediated CaMKKβ/AMPK signaling in cisplatin-induced mice and TECs.These findings highlight the original role of tubular FFAR4 in cellular senescence via AMPK/SirT3 signaling and identify FFAR4 as a potential drug target against AKI. | Letian Yang Bo Wang Fan Guo Rongshuang Huang Yan Liang Lingzhi Li Sibei Tao Ting Yin Ping Fu Liang Ma | 2022 | Signal Transduction and Targeted Therapy2022，7，12： | 0 |
| 10 | Crystallographic Characterization and Themal Decomposition Behavior of High-Purity Microcrystalline Magnesite显示文摘The intergranular cracking face is smooth and transgranular cracking fracture shows trace of cleavage. It is hard to find characteristic rhombus in the dense small crystals.As measured by SEM, the particle size of microcrystalline magnesite originating in Sichuan-Tibet is uniform, most grains range in 2-4 μm. Three-dimensional (3D) crystal morphology assumes cubic or column. The result of chemical analysis shows that the microcrystalline magnesite has high purity and XRD indicates that the lattice constant is the same as macrocrystalline magnesite with standard d values and X-ray diffraction intensity. The decomposition temperature is 650 ℃ determined by DTA, which is also the same as macrocrystalline magnesite. Crystal morphology of nanometer periclase treated at 650 ℃ for 4 h and calcined at 1 000 ℃ respectively was observed by FE-SEM, the grain of periclase fired at 650 ℃ is <20 nm and that of the periclase calcined at 1 000 ℃ is <200 nm, both are round. The quality of high purity magnesite manufactured by high temperature shaft kiln is at the same level as sea water magnesite made in Japan and brine magnesite produced in the Netherlands, while the crystal size is slightly larger. | HE Zhongyang MA Letian LIU Baikuan | 2012 | China's Refractories2012，21，3： | 0 |
| 11 | Metabolic and immunological effects of gut microbiota in leaf beetles at the local and systemic levels显示文摘Insects’intestinal microbes have profound effects on the host’s physiological traits,which can impact their physiology at both the local(gut)and systemic(body)levels.Nevertheless,the molecular mechanisms underlying hostmicrobiota interactions,especially in non-model insects,remain elusive.Recently,tissue-specific transcriptomic analysis has been highlighted as a robust tool in studying host–microbe interactions.Plagiodera versicolora is a worldwide leaf-eating pest that primarily feeds on willows and poplar.The interplay between gut microflora and this host beetle has yet to be studied.Herein,we investigate the effects of the gut microbiota on the body mass of P.versicolora larvae,compare the nutrition status of larvae in absence and presence of gut microbiota,and profile gut bacterial loads throughout its developmental larval stages.We then perform comparative transcriptomic analysis of gut and body tissues in axenic and non-axenic larvae.Finally,we confirm the expression patterns of representative genes in nutritional metabolism and immunity.Results show that weight growth is retarded in conventional larvae,with a concomitant increase of total bacterial load by the 5^(th)development day,and germ-free larvae have a higher glucose content than conventional-reared larvae.Both nutritional and immunological analyses indicate that gut bacteria are a burden in the beetle’s larval development.These findings elucidate the impacts of gut microbiota on P.versicolora,and provide insight into tissue-specific responses to gut microflora in this pest at the genetic level,boosting our understanding of the molecular mechanisms underlying host–microbe interactions in leaf beetles and beyond. | Meiqi MA Chengjie TU Jing LUO Min LU Shichang ZHANG Letian XU | 2021 | Integrative Zoology2021，16，3： | 0 |
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