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| 1 | Remote heart rate measurement using low-cost RGB face video: a technical literature review显示文摘 | Philipp V. ROUAST Marc T. P. ADAM Raymond CHIONG David CORNFORTH Ewa LUX | 2018 | Frontiers of Computer Science2018，12，5： | 12 |
| 2 | 多年生黑麦草叶片长度数量性状位点(QTLs)研究显示文摘选择固定有益等位基因 ,研究多年生黑麦草叶片长度数量性状位点 ,结果表明 :与叶长最为相关的 3个标记所表达的变异占表型总变异的 5 0 .9% (r2 ) ;在选择中如能将这 3个标记最优化地组合在一起 ,其综合共同作用有望使叶片长度延长和缩短 2 6 mm;鉴于供试材料在最初 3个世代的群体选择中所估测到叶片长度的遗传力为 0 .32 ,这样在今后的选择工作中 ,即使只使用现有的两个标记 。 | 米福贵 BARRE Philippe 瞿礼嘉 GHESQUIERE Marc | 2004 | 草地学报2004，12，4： | 7 |
| 3 | Hepatitis B virus genotypes and extrahepatic manifestations显示文摘 | Patrice Cacoub David Saadoun Marc Bourlière Hacene Khiri Agnes Martineau Yves Benhamou Marina Varastet Stanislas Pol Vincent Thibault Michel Rotily Philippe Halfon | 2005 | Journal of Hepatology2005，，5： | 5 |
| 4 | Comparison of conventional radiography and MDCT in suspected scaphoid fractures显示文摘AIM: To determine the diagnostic accuracy and radiation dose of conventional radiography and multidetector computed tomography(MDCT) in suspected scaphoid fractures.METHODS: One hundred twenty-four consecutive patients were enrolled in our study who had suffered from a wrist trauma and showed typical clinical symptoms suspicious of an acute scaphoid fracture. All patients had initially undergone conventional radiography. Subsequent MDCT was performed within 10 d because of persisting clinical symptoms. Using the MDCT data as the reference standard, a fourfold table was used to classify the test results. The effective dose and impaired energy were assessed in order to compare the radiation burden of the two techniques. The Wilcoxon test was performed to compare the two diagnostic modalities.RESULTS: Conventional radiography showed 34 acute fractures of the scaphoid in 124 patients(42.2%). Subsequent MDCT revealed a total of 42 scaphoid fractures. The sensitivity of conventional radiography for scaphoid fracture detection was 42.8% and its specificity was 80% resulting in an overall accuracy of 59.6%. Conventional radiography was significantly inferior to MDCT(P < 0.01) concerning scaphoidfracture detection. The mean effective dose of MDCT was 0.1 m Sv compared to 0.002 m Sv of conventional radiography.CONCLUSION: Conventional radiography is insufficient for accurate scaphoid fracture detection. Regarding the almost negligible effective dose, MDCT should serve as the first imaging modality in wrist trauma. | Cyrus Behzadi Murat Karul Frank Oliver Henes Azien Laqmani Philipp Catala-Lehnen Wolfgang Lehmann Hans-Dieter Nagel Gerhard Adam Marc Regier | 2015 | World Journal of Radiology2015，7，1： | 4 |
| 5 | Severe delayed complication after percutaneous endoscopic colostomy for chronic intestinal pseudo-obstruction: A case report and review of the literature显示文摘经皮的内视镜的结肠开口术(PEC ) 逐渐地作为外科的一种选择被建议对待各种各样的混乱,包括尖锐结肠的伪阻塞,长期的肠的伪阻塞和复发的 sigmoid 肠扭传。我们在 PEC 放置以后在发生的严重复杂并发症上报导二个月。有自从 8 年起,演变的长期的肠的伪阻塞的历史的一个 74 岁的人被重新接纳到我们的医院并且收到了 PEC 提供长期的地势。过程是平静的并且极大地改进了病人生命的质量。二个月以后,病人开发了尖锐粪的腹膜炎。在剖腹术,结肠开口术凸缘在腹壁被嵌入,但是没有压迫性坏死在结肠的墙的水平被发现。这复杂并发症与结肠开口术试管的不经心的拖拉有关是可能的。有终端回肠造口术的小计结肠切除术被执行。我们考察迄今为止报导的 PEC 的 60 个案例的主要特征,包括指示和复杂并发症。 | David Bertolini Philippe De Saussure Michael Chilcott Marc Girardin Jean-Marc Dumonceau | 2007 | World Journal of Gastroenterology2007，13，15： | 4 |
| 6 | Rabeprazole test for the diagnosis of gastro-oesophageal reflux disease: Results of a study in a primary care setting显示文摘瞄准:为了决定病人的 rabeprazole 测试的诊断价值,由全科医生看。方法:有 GERD 暗示的症状的 83 个病人被全科医生在这个多中心注册,使随机化并且双窗帘学习。所有病人收到了 rabeprazole (20 mg 出价) 或安慰剂一个星期。GERD 的诊断在内视镜检查法或反常食道的 24-h pH 测试在粘膜裂缝的存在上被建立。如果病人们报导至少,“ 7 点 Likert 上的症状的清楚的改进”可伸缩,测试被认为阳性。结果:为 rabeprazole 和安慰剂的测试的敏感分别地是 83% 和 40% 。相应特性,积极、否定的预兆的价值是 45% 和 67% , 71% 和 71% ,和 62% 和 35% 分别地。操作特征(巨鸟) 分析的接收装置证实最好差别对待截止对应于对“清楚的改进”的描述。结论:禁止者(PPI ) 测试的质子泵的差的特性不支持如此的一条途径在一个主要照顾背景建立 GERD 的诊断。 | Stanislas Bruley des Varannes Sylvie Sacher-Huvelin Fabienne Vavasseur Claude Masliah Marc Le Rhun Philippe Aygalenq Sylvie Bonnot-Marlier Yves Lequeux Jean Paul Galmiche | 2006 | World Journal of Gastroenterology2006，12，16： | 4 |
| 7 | Large endoscopic mucosal resection for colorectal tumors exceeding 4 cm显示文摘AIM:To evaluate the feasibility and the outcome of endoscopic mucosal resection(EMR)for large colorectal tumors exceeding 4 cm(LCRT)undergoing piecemeal resection. METHODS:From January 2005 to April 2008,146 digestive tumors larger than 2 cm were removed with the EMR technique in our department.Of these,34 tumors were larger than 4 cm and piecemeal resection was carried out on 26 colorectal tumors.The mean age of the patients was 71 years.The mean follow-up duration was 12 mo. RESULTS:LCRTs were located in the rectum,left colon,transverse colon and right colon in 58%,15%, 4%and 23%of cases,respectively.All were sessile tumors larger than 4 cm with a mean size of 4.9 cm (4-10 cm).According to the Paris classification,34%of the tumors were typeⅠs,58%typeⅡa,4%typeⅡb and 4%typeⅡc.Pathological examination showed tubulous adenoma in 31%,tubulo-villous adenoma in 27%,villous adenoma in 42%,high-grade dysplasia in38%,in situ carcinoma in 19%of the cases and mucosal carcinoma(m2)in 8%of the cases.The two cases(7.7%)of procedural bleeding that occurred were managed endoscopically and one small perforation was treated with clips.During follow-up,recurrence of the tumor occurred in three patients(12%),three of whom received endoscopic treatment. CONCLUSION:EMR for tumors larger than 4 cm is a safe and effective procedure that could compete with endoscopic submucosal dissection,despite providing incomplete histological assessment. | Philippe Ah Soune Charles Ménard Ezzedine Salah Ariadne Desjeux Jean-Charles Grimaud Marc Barthet | 2010 | World Journal of Gastroenterology2010，16，5： | 4 |
| 8 | Increased Risk for Nonmelanoma Skin Cancers in Patients Who Receive Thiopurines for Inflammatory Bowel Disease显示文摘 | Laurent Peyrin–Biroulet Kiarash Khosrotehrani Fabrice Carrat Anne–Marie Bouvier Jean–Baptiste Chevaux Tabassome Simon Frank Carbonnel Jean–Frédéric Colombel Jean–Louis Dupas Philippe Godeberge Jean–Pierre Hugot Marc Lémann Stéphane Nahon Jean–Marc Sabaté | 2011 | Gastroenterology2011，，5： | 3 |
| 9 | Dual-color deep-tissue three-photon microscopy with a multiband infrared laser显示文摘Multiphoton microscopy combined with genetically encoded fluorescent indicators is a central tool in biology.Threephoton(3P)microscopy with excitation in the short-wavelength infrared(SWIR)water transparency bands at 1.3 and 1.7μm opens up new opportunities for deep-tissue imaging.However,novel strategies are needed to enable in-depth multicolor fluorescence imaging and fully develop such an imaging approach.Here,we report on a novel multiband SWIR source that simultaneously emits ultrashort pulses at 1.3 and 1.7μm that has characteristics optimized for 3P microscopy:sub-70 fs duration,1.25 MHz repetition rate,and μJ-range pulse energy.In turn,we achieve simultaneous 3P excitation of green fluorescent protein(GFP)and red fluorescent proteins(mRFP,mCherry,tdTomato)along with third-harmonic generation.We demonstrate in-depth dual-color 3P imaging in a fixed mouse brain,chick embryo spinal cord,and live adult zebrafish brain,with an improved signal-to-background ratio compared to multicolor twophoton imaging.This development opens the way towards multiparametric imaging deep within scattering tissues. | Khmaies Guesmi Lamiae Abdeladim Samuel Tozer Pierre Mahou Takuma Kumamoto Karolis Jurkus Philippe Rigaud Karine Loulier Nicolas Dray Patrick Georges Marc Hanna Jean Livet Willy Supatto Emmanuel Beaurepaire Frédéric Druon | 2018 | Light(Science & Applications)2018，7，1： | 3 |
| 10 | E-Cadherin loss associated with EMT promotes radioresistance in human tumor cells显示文摘 | Jan Theys Barry Jutten Roger Habets Kim Paesmans Arjan J. Groot Philippe Lambin Brad G. Wouters Guido Lammering Marc Vooijs | 2011 | Radiotherapy and Oncology2011，，3： | 3 |
| 11 | Towards a pan-European consensus on the treatment of patients with colorectal liver metastases显示文摘 | Eric Van Cutsem Bernard Nordlinger Rene Adam Claus-Henning K?hne Carmelo Pozzo Graeme Poston Marc Ychou Philippe Rougier | 2006 | European Journal of Cancer2006，，14： | 2 |
| 12 | <ce:link locator='fx1'/> A Review of Activity Indices and Efficacy End Points for Clinical Trials of Medical Therapy in Adults With Ulcerative Colitis显示文摘 | Geert D’Haens William J. Sandborn Brian G. Feagan Karel Geboes Stephen B. Hanauer E. Jan Irvine Marc Lémann Philippe Marteau Paul Rutgeerts Jurgen Sch?lmerich Lloyd R. Sutherland | 2007 | Gastroenterology2007，，2： | 2 |
| 13 | Fungal diversity notes 111-252-taxonomic and phylogenetic contributions to fungal taxa显示文摘This paper is a compilation of notes on 142 fungal taxa,including five new families,20 new genera,and 100 new species,representing a wide taxonomic and geographic range.The new families,Ascocylindricaceae,Caryosporaceae and Wicklowiaceae(Ascomycota)are introduced based on their distinct lineages and unique morphology.The new Dothideomycete genera Pseudomassariosphaeria(Amniculicolaceae),Heracleicola,Neodidymella and Pseudomicrosphaeriopsis(Didymellaceae),Pseudopithomyces(Didymosphaeriaceae),Brunneoclavispora,Neolophiostoma and Sulcosporium(Halotthiaceae),Lophiohelichrysum(Lophiostomataceae),Galliicola,Populocrescentia and Vagicola(Phaeosphaeriaceae),Ascocylindrica(Ascocylindricaceae),Elongatopedicellata(Roussoellaceae),Pseudoasteromassaria(Latoruaceae)and Pseudomonodictys(Macrodiplodiopsidaceae)are introduced.The newly described species of Dothideomycetes(Ascomycota)are Pseudomassariosphaeria bromicola(Amniculicolaceae),Flammeascoma lignicola(Anteagloniaceae),Ascocylindrica marina(Ascocylindricaceae),Lembosia xyliae(Asterinaceae),Diplodia crataegicola and Diplodia galiicola(Botryosphaeriaceae),Caryospora aquatica(Caryosporaceae),Heracleicola premilcurensis and Neodidymella thailandicum(Didymellaceae),Pseudopithomyces palmicola(Didymosphaeriaceae),Floricola viticola(Floricolaceae),Brunneoclavispora bambusae,Neolophiostoma pigmentatum and Sulcosporium thailandica(Halotthiaceae),Pseudoasteromassaria fagi(Latoruaceae),Keissleriella dactylidicola(Lentitheciaceae),Lophiohelichrysum helichrysi(Lophiostomataceae),Aquasubmersa japonica(Lophiotremataceae),Pseudomonodictys tectonae(Macrodiplodiopsidaceae),Microthyrium buxicola and Tumidispora shoreae(Microthyriaceae),Alloleptosphaeria clematidis,Allophaeosphaeria cytisi,Allophaeosphaeria subcylindrospora,Dematiopleospora luzulae,Entodesmium artemisiae,Galiicola pseudophaeosphaeria,Loratospora luzulae,Nodulosphaeria senecionis,Ophiosphaerella aquaticus,Populocrescentia forlicesenensis and Vagicola vagans(Phaeosphaeriaceae),Elongatopedicellata lignicola,Roussoella magnatum and Roussoella angustior(Roussoellaceae)and Shrungabeeja longiappendiculata(Tetraploasphaeriaceae).The new combinations Pseudomassariosphaeria grandispora,Austropleospora archidendri,Pseudopithomyces chartarum,Pseudopithomyces maydicus,Pseudopithomyces sacchari,Vagicola vagans,Punctulariopsis cremeoalbida and Punctulariopsis efibulata Dothideomycetes.The new genera Dictyosporella(Annulatascaceae),and Tinhaudeus(Halosphaeriaceae)are introduced in Sordariomycetes(Ascomycota)while Dictyosporella aquatica(Annulatascaceae),Chaetosphaeria rivularia(Chaetosphaeriaceae),Beauveria gryllotalpidicola and Beauveria loeiensis(Cordycipitaceae),Seimatosporium sorbi and Seimatosporium pseudorosarum(Discosiaceae),Colletotrichum aciculare,Colletotrichum fusiforme and Colletotrichum hymenocallidicola(Glomerellaceae),Tinhaudeus formosanus(Halosphaeriaceae),Pestalotiopsis subshorea and Pestalotiopsis dracaenea(Pestalotiopsiceae),Phaeoacremonium tectonae(Togniniaceae),Cytospora parasitica and Cytospora tanaitica(Valsaceae),Annulohypoxylon palmicola,Biscogniauxia effusae and Nemania fusoideis(Xylariaceae)are introduced as novel species to order Sordariomycetes.The newly described species of Eurotiomycetes are Mycocalicium hyaloparvicellulum(Mycocaliciaceae).Acarospora septentrionalis and Acarospora castaneocarpa(Acarosporaceae),Chapsa multicarpa and Fissurina carassensis(Graphidaceae),Sticta fuscotomentosa and Sticta subfilicinella(Lobariaceae)are newly introduced in class Lecanoromycetes.In class Pezizomycetes,Helvella pseudolacunosa and Helvella rugosa(Helvellaceae)are introduced as new species.The new families,Dendrominiaceae and Neoantrodiellaceae(Basidiomycota)are introduced together with a new genus Neoantrodiella(Neoantrodiellaceae),here based on both morphology coupled with molecular data.In the class Agaricomycetes,Agaricus pseudolangei,Agaricus haematinus,Agaricus atrodiscus and Agaricus exilissimus(Agaricaceae),Amanita melleialba,Amanita pseudosychnopyramis and Amanita subparvipantherina(Amanitaceae),Entoloma calabrum,Cora barbulata,Dictyonema gomezianum and Inocybe granulosa(Inocybaceae),Xerocomellus sarnarii(Boletaceae),Cantharellus eucalyptorum,Cantharellus nigrescens,Cantharellus tricolor and Cantharellus variabilicolor(Cantharellaceae),Cortinarius alboamarescens,Cortinarius brunneoalbus,Cortinarius ochroamarus,Cortinarius putorius and Cortinarius seidlii(Cortinariaceae),Hymenochaete micropora and Hymenochaete subporioides(Hymenochaetaceae),Xylodon ramicida(Schizoporaceae),Colospora andalasii(Polyporaceae),Russula guangxiensis and Russula hakkae(Russulaceae),Tremella dirinariae,Tremella graphidis and Tremella pyrenulae(Tremellaceae)are introduced.Four new combinations Neoantrodiella gypsea,Neoantrodiella thujae(Neoantrodiellaceae),Punctulariopsis cremeoalbida,Punctulariopsis efibulata(Punctulariaceae)are also introduced here for the division Basidiomycota.Furthermore Absidia caatinguensis,Absidia koreana and Gongronella koreana(Cunninghamellaceae),Mortierella pisiformis and Mortierella formosana(Mortierellaceae)are newly introduced in the Zygomycota,while Neocallimastix cameroonii and Piromyces irregularis(Neocallimastigaceae)are introduced in the Neocallimastigomycota.Reference specimens or changes in classification and notes are provided for Alternaria ethzedia,Cucurbitaria ephedricola,Austropleospora,Austropleospora archidendri,Byssosphaeria rhodomphala,Lophiostoma caulium,Pseudopithomyces maydicus,Massariosphaeria,Neomassariosphaeria and Pestalotiopsis montellica. | Hiran A.Ariyawansa Kevin D.Hyde Subashini C.Jayasiri Bart Buyck K.W.Thilini Chethana Dong Qin Dai Yu Cheng Dai Dinushani A.Daranagama Ruvishika S.Jayawardena Robert Lücking Masoomeh Ghobad-Nejhad Tuula Niskanen Kasun M.Thambugala Kerstin Voigt Rui Lin Zhao Guo-Jie Li Mingkwan Doilom Saranyaphat Boonmee Zhu L.Yang Qing Cai Yang-Yang Cui Ali H.Bahkali Jie Chen Bao Kai Cui Jia Jia Chen Monika C.Dayarathne Asha J.Dissanayake Anusha H.Ekanayaka Akira Hashimoto Sinang Hongsanan E.B.Gareth Jones Ellen Larsson Wen Jing Li Qi-Rui Li Jian Kui Liu Zong Long Luo Sajeewa S.N.Maharachchikumbura Ausana Mapook Eric H.C.McKenzie Chada Norphanphoun Sirinapa Konta Ka Lai Pang Rekhani H.Perera Rungtiwa Phookamsak Chayanard Phukhamsakda Umpava Pinruan Emile Randrianjohany Chonticha Singtripop Kazuaki Tanaka Cheng Ming Tian Saowaluck Tibpromma Mohamed A.Abdel-Wahab Dhanushka N.Wanasinghe Nalin N.Wijayawardene Jin-Feng Zhang Huang Zhang Faten A.Abdel-Aziz Mats Wedin Martin Westberg Joseph F.Ammirati Timur S.Bulgakov Diogo X.Lima Tony M.Callaghan Philipp Callac Cheng-Hao Chang Luis F.Coca Manuela Dal-Forno Veronika Dollhofer Kateřina Fliegerová Katrin Greiner Gareth W.Griffith Hsiao-Man Ho Valerie Hofstetter Rajesh Jeewon Ji Chuan Kang Ting-Chi Wen Paul M.Kirk Ilkka Kytövuori James D.Lawrey Jia Xing Hong Li Zou Yi Liu Xing Zhong Liu Kare Liimatainen H.Thorsten Lumbsch Misato Matsumura Bibiana Moncada Salilaporn Nuankaew Sittiporn Parnmen AndréL.C.M.de Azevedo Santiago Sujinda Sommai Yu Song Carlos A.F.de Souza Cristina M.de Souza-Motta Hong Yan Su Satinee Suetrong Yong Wang Syuan-Fong Wei Ting Chi Wen Hai Sheng Yuan Li Wei Zhou Martina Réblová Jacques Fournier Erio Camporesi J.Jennifer Luangsa-ard Kanoksri Tasanathai Artit Khonsanit Donnaya Thanakitpipattana Sayanh Somrithipol Paul Diederich Ana M.Millanes Ralph S.Common Marc Stadler Ji Ye Yan XingHong Li Hye Won Lee Thi T.T.Nguyen Hyang Burm Lee Eliseo Battistin Orlando Marsico Alfredo Vizzini Jordi Vila Enrico Ercole Ursula Eberhardt Giampaolo Simonini Hua-An Wen Xin-Hua Chen Otto Miettinen Viacheslav Spirin Hernawati | 2015 | Fungal Diversity2015，，6： | 2 |
| 14 | Hepatic and Extrahepatic Colorectal Metastases: When Resectable, Their Localization Does Not Matter, But Their Total Number Has a Prognostic Effect显示文摘 | Dominique Elias MD PhD Gabriel Liberale MD Déwi Vernerey MSc Marc Pocard MD PhD Michel Ducreux MD PhD Valérie Boige MD David Malka MD PhD Jean-Pierre Pignon MD PhD Philippe Lasser MD | 2005 | Annals of Surgical Oncology2005，，11： | 2 |
| 15 | Proteomic and metabolomic profiling underlines the stage- and time-dependent effects of high temperature on grape berry metabolism显示文摘Climate change scenarios predict an increase in mean air temperatures and in the frequency,intensity,and length of extreme temperature events in many wine-growing regions worldwide.Because elevated temperature has detrimental effects on berry growth and composition,it threatens the economic and environmental sustainability of wine production.Using Cabernet Sauvignon fruit-bearing cuttings,we investigated the effects of high temperature(HT)on grapevine berries through a label-free shotgun proteomic analysis coupled to a complementary metabolomic study.Among the 2,279 proteins identified,592 differentially abundant proteins were found in berries exposed to HT.The gene ontology categories“stress,”“protein,”“secondary metabolism,”and“cell wall”were predominantly altered under HT.High temperatures strongly impaired carbohydrate and energy metabolism,and the effects depended on the stage of development and duration of treatment.Transcript amounts correlated poorly with protein expression levels in HT berries,highlighting the value of proteomic studies in the context of heat stress.Furthermore,this work reveals that HT alters key proteins driving berry development and ripening.Finally,we provide a list of differentially abundant proteins that can be considered as potential markers for developing or selecting grape varieties that are better adapted to warmer climates or extreme heat waves. | David Lecourieux Christian Kappel Stéphane Claverol Philippe Pieri Regina Feil John E.Lunn Marc Bonneu Lijun Wang Eric Gomès Serge Delrot Fatma Lecourieux | 2020 | Journal of Integrative Plant Biology2020，62，8： | 2 |
| 16 | Adipokines Linking Obesity with Colorectal Cancer Risk in Postmenopausal Women显示文摘 | Gloria Y.F. Ho Tao Wang Marc J. Gunter Howard D. Strickler Mary Cushman Robert C. Kaplan Sylvia Wassertheil-Smoller Xiaonan Xue Swapnil N. Rajpathak Rowan T. Chlebowski Mara Z. Vitolins Philipp E. Scherer Thomas E. Rohan | 2012 | Cancer Research2012，，12： | 2 |
| 17 | Bacillus lipopeptides: versatile weapons for plant disease biocontrol显示文摘 | Marc Ongena Philippe Jacques | 2007 | Trends in Microbiology2007，，3： | 2 |
| 18 | Value of pelvic embolization in the management of severe postpartum hemorrhage due to placenta accreta, increta or percreta显示文摘 | Philippe Soyer Olivier Morel Yann Fargeaudou Marc Sirol Fabrice Staub Mourad Boudiaf Henri Dahan Alexandre Mebazaa Emmanuel Barranger Olivier le Dref | 2010 | European Journal of Radiology2010，，3： | 2 |
| 19 | <ce:link locator='fx1'/> A Review of Activity Indices and Efficacy End Points for Clinical Trials of Medical Therapy in Adults With Ulcerative Colitis显示文摘 | Geert D'Haens William J. Sandborn Brian G. Feagan Karel Geboes Stephen B. Hanauer E. Jan Irvine Marc Lémann Philippe Marteau Paul Rutgeerts Jurgen Sch?lmerich Lloyd R. Sutherland | 2007 | Gastroenterology2007，，2： | 2 |
| 20 | Methylome and transcriptome data integration reveals potential roles of DNA methylation and candidate biomarkers of cow Streptococcus uberis subclinical mastitis显示文摘Background:Mastitis caused by different pathogens including Streptococcus uberis(S.uberis)is responsible for huge economic losses to the dairy industry.In order to investigate the potential genetic and epigenetic regulatory mecha‑nisms of subclinical mastitis due to S.uberis,the DNA methylome(whole genome DNA methylation sequencing)and transcriptome(RNA sequencing)of milk somatic cells from cows with naturally occurring S.uberis subclinical mastitis and healthy control cows(n=3/group)were studied.Results:Globally,the DNA methylation levels of CpG sites were low in the promoters and first exons but high in inner exons and introns.The DNA methylation levels at the promoter,first exon and first intron regions were nega‑tively correlated with the expression level of genes at a whole‑genome‑wide scale.In general,DNA methylation level was lower in S.uberis‑positive group(SUG)than in the control group(CTG).A total of 174,342 differentially methylated cytosines(DMCs)(FDR<0.05)were identified between SUG and CTG,including 132,237,7412 and 34,693 DMCs in the context of CpG,CHG and CHH(H=A or T or C),respectively.Besides,101,612 methylation haplotype blocks(MHBs)were identified,including 451 MHBs that were significantly different(dMHB)between the two groups.A total of 2130 differentially expressed(DE)genes(1378 with up‑regulated and 752 with down‑regulated expression)were found in SUG.Integration of methylome and transcriptome data with MethGET program revealed 1623 genes with signifi‑cant changes in their methylation levels and/or gene expression changes(MetGDE genes,MethGET P‑value<0.001).Functional enrichment of genes harboring≥15 DMCs,DE genes and MetGDE genes suggest significant involvement of DNA methylation changes in the regulation of the host immune response to S.uberis infection,especially cytokine activities.Furthermore,discriminant correlation analysis with DIABLO method identified 26 candidate biomarkers,including 6 DE genes,15 CpG‑DMCs and 5 dMHBs that discriminated between SUG and CTG.Conclusion:The integration of methylome and transcriptome of milk somatic cells suggests the possible involve‑ment of DNA methylation changes in the regulation of the host immune response to subclinical mastitis due to S.uberis.The presented genetic and epigenetic biomarkers could contribute to the design of management strategies of subclinical mastitis and breeding for mastitis resistance. | Mengqi Wang Nathalie Bissonnette Mario Laterriere Pier‑Luc Dudemaine David Gagne Jean‑Philippe Roy Xin Zhao Marc‑Andre Sirard Eveline M.Ibeagha‑Awemu | 2023 | Journal of Animal Science and Biotechnology2023，14，2： | 2 |
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