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| 1 | Sirolimus inhibits growth of human hepatoma cells alone or combined with tacrolimus, while tacrolimus promotes cell growth显示文摘AIM: Standard immunosuppression after organ transplantation stimulates tumor growth. Sirolimus has a strong antiproliferative and a tumor inhibiting effect. The purpose is to assess the effect on tumor growth of the immunosuppressive compounds sirolimus and tacrolimus alone and in combination on cells of human hepatocellular carcinoma.METHODS: We used the human cell lines SK-Hep 1 and Hep 3B derived from hepatocellular carcinoma. Proliferation analyses after treatment with sirolimus, tacrolimus, or the combination of both were performed. FACS analyses were done to reveal cell cycle changes and apoptotic cell death. The expression of apoptosis-related proteins was estimated by Western blots.RESULTS: Sirolimus alone or combined with tacrolimus inhibited the growth of both cell lines after 5 d by up to 35% in SK-Hep 1 cells, and by up to 68% in Hep 3B cells at 25 ng/mL. Tacrolimus alone stimulated the growth by 12% after 5 ng/mL and by 25% after 25 ng/mL in Hep 3B cells. We found an increase of apoptotic Hep 3B cells from 6 to 16%, and a G1-arrest in SK-Hep 1 cells with an increase of cells from 61 to 82%, when sirolimus and tacrolimus were combined. Bcl-2 was down-regulated in Hep 3B, but not in SK-Hep 1 cells after combined treatment.CONCLUSION: Sirolimus appears to inhibit the growth of hepatocellular carcinoma cells alone and in combination with tacrolimus. Sirolimus seems to inhibit the growth stimulation of tacrolimus. | Guido Schumacher Marijke Oidtmann Anne Rueggeberg Dietmar Jacob Sven Jonas Jan M. Langrehr Ruth Neuhaus Marcus Bahra Peter Neuhaus | 2005 | World Journal of Gastroenterology2005，11，10： | 14 |
| 2 | Matched-pair analysis of postoperative morbidity and mortality for pancreaticogastrostomy and pancreaticojejunostomy using mattress sutures in soft pancreatic tissue remnants显示文摘BACKGROUND:After pancreaticoduodenectomy,the incidence of postoperative pancreatic fistula remains high,especially in patients with 'soft' pancreatic tissue remnants.No 'gold standard' surgical technique for pancreaticoenteric anastomosis has been established.This study aimed to compare the postoperative morbidity and mortality of pancreaticogastrostomy and pancreaticojejunostomy for 'soft' pancreatic tissue remnants using modified mattress sutures.METHODS:Seventy-five patients who had undergone pancreaticogastrostomy and 75 who had undergone pancreaticojejunostomy after pancreaticoduodenectomy between 2002 and 2008 were retrospectively compared using matched-pair analysis.A modified mattress suture technique was used for the pancreaticoenteric anastomosis.Patients with an underlying 'hard' pancreatic tissue remnant,as in chronic pancreatitis,were excluded.Both groups were homogeneous for age,gender,and underlying disease.Postoperative morbidity,mortality,and preoperative and operative data were analyzed.RESULTS:There were no significant differences between the groups for the incidence of postoperative pancreatic fistula (10.7% in both).Postoperative morbidity and mortality,median operation time,median length of hospital stay,intraoperative blood loss,and the amount of intraoperatively transfused erythrocyte concentrates also did not significantly differ between the groups.Patient age >65 years (P=0.017),operation time >350minutes (P=0.001),and intraoperative transfusion of erythrocyte concentrates (P=0.038) were identified as risk factors for postoperative morbidity.CONCLUSIONS:Our results showed no significant differences between the groups in the pancreaticogastrostomy and pancreaticojejunostomy anastomosis techniques using mattress sutures for 'soft' pancreatic tissue remnants.In our experience,the mattress sutures are safe and simple to use,and pancreaticogastrostomy in particular is feasible and easy to learn,with good endoscopic accessibility to the anastomosis region.However,the location of the anastomosis and the surgical technique need to be individually evaluated to further reduce the incidence of postoperative pancreatic fistula. | Fritz Klein Marcus Bahra Matthias Glanemann Wladimir Faber Peter Warnick Andreas Andreou Safak Gül Dietmar Jacob | 2012 | Hepatobiliary & Pancreatic Diseases International2012，11，1： | 9 |
| 3 | Different cava reconstruction techniques in liver transplantation:piggyback versus cava resection显示文摘BACKGROUND: Originally, cava reconstruction(CR) in liver transplantation meant complete resection and reinsertion of the donor cava. Alternatively, preservation of the recipients inferior vena cava(IVC) with side-to-side anastomosis(known as 'piggyback') can be performed. Here, partial clamping maintains blood flow of the IVC, which may improve cardiovascular stability, reduce blood loss and stabilize kidney function. The aim of this study was to compare both techniques with particular focus on kidney function.METHODS: A series of 414 patients who had had adult liver transplantations(2006-2009) were included. Among them,176(42.5%) patients had piggyback and 238 had classical CR operation, 112(27.1%) of the patients underwent CR accompanied with veno-venous bypass(CR-B) and 126(30.4%)without a bypass. The choice of either technique was based on the surgeons' individual preference. Kidney function [serum creatinine, calculated glomerular filtration rate(GFR), RIFLE stages] was assessed over 14 days.RESULTS: Lab-MELD scores were significantly higher in CR-B(22.5±11.0) than in CR(17.3±9.0) and piggyback(18.8±10.0)(P=0.008). Unexpectedly, the incidences of arterial stenoses(P=0.045) and biliary leaks(P=0.042) were significantly increased in piggyback. Preoperative serum creatinine levels were the highest in CR-B [1.45±1.17 vs 1.25±0.85(piggyback)and 1.13±0.60 mg/dL(CR); P=0.033]. Although a worsening of postoperative kidney function was observed among all groups,this was most pronounced in CR-B [creatinine day 14: 1.67±1.40 vs 1.35±0.96(piggyback) and 1.45±1.03 mg/dL(CR); P=0.102].Accordingly, the proportion of patients displaying RIFLE stages≥2 was the highest in CR/CR-B(26%/19%) when compared to piggyback(18%).CONCLUSIONS: Piggyback revealed a shorter warm ischemic time, a reduced blood loss, and a decreased risk of acute kidney failure. Thus, piggyback is a useful technique, which should be applied in standard procedures. When piggyback is unfeasible,cava replacement, which displayed a lower incidence of vascular and biliary complications in our study, remains as a safe alternative. | Volker Schmitz Wenzel Schoening Ines Jelkmann Brigitta Globke Andreas Pascher Marcus Bahra Peter Neuhaus Gero Puhl | 2014 | Hepatobiliary & Pancreatic Diseases International2014，13，3： | 5 |
| 4 | Perioperative treatment options in resectable pancreatic cancer-how to improve long-term survival显示文摘Surgery remains the only chance of cure for pancreatic cancer, but only 15%-25% of patients present with resectable disease at the time of primary diagnosis. Important goals in clinical research must therefore be to allow early detection with suitable diagnostic procedures, to further broaden operation techniques and to determine the most effective perioperative treatment of either chemotherapy and/or radiation therapy. More extensive operations involving extended pancreatectomy, portal vein resection and pancreatic resection in resectable pancreatic cancer with limited liver metastasis, performed in specialized centers seem to be the surgical procedures with a possible impact on survival. After many years of stagnation in pharmacological clinical research on advanced pancreatic ductal adenocarcinomas(PDAC)- since the approval of gemcitabine in 1997- more effective cytotoxic substances(nab-paclitaxel) and combinations(FOLFIRINOX) are now available for perioperative treatment. Additionally, therapies with a broader mechanism of action are emerging(stroma depletion, immunotherapy, antiinflammation), raising hopes for more effective adjuvant and neoadjuvant treatment concepts, especially in the context of 'borderline resectability'. Only multidisciplinary approaches including radiology, surgery, medical and radiation oncology as the backbones of the treatment of potentially resectable PDAC may be able to further improve the rate of cure in the future. | Marianne Sinn Marcus Bahra Timm Denecke Sue Travis Uwe Pelzer Hanno Riess | 2016 | World Journal of Gastrointestinal Oncology2016，8，3： | 3 |
| 5 | Tumor–stromal cross-talk modulating the therapeutic response in pancreatic cancer显示文摘Background: Pancreatic ductal adenocarcinoma(PDAC) is a highly malignant solid tumor with a dismal prognosis. The stroma component makes up to 90% of the tumor mass and is thought to be one of the main reasons for the tumor's high chemoresistance. Cancer associated fibroblasts(CAFs) have previously been identified to be the key stromal players. This is the first time we provide detailed in vitro experiments investigating tumor–stromal interactions when exposed to three well-known chemotherapeutic agents. Methods: Monocultures, indirect and direct co-cultures of two PDAC cell lines(AsPC and Panc-1) and six primary patients derived CAFs were treated with gemcitabine, nab-paclitaxel and the γ-secretaseinhibitor(GSI) DAPT. The cell viability of each component was measured with XTT. Finally, IL-6 concentrations of the supernatants were analyzed. Results: On the contrary to PDAC cell lines, CAF monocultures hardly responded to any treatment which suggested that stroma(CAFs) itself is more resistant to standard chemo-treatments than the epithelial cancer cells. Moreover, only a weak chemotherapeutic response was observed in direct co-cultures of cancer cells with CAFs. A change in the morphology of direct co-cultures was accompanied with the chemoresistance. CAFs were observed to build cage-like structures around agglomerates of tumor cells. High levels of IL-6 were also associated with a reduced response to therapy. Indirect co-cultures make the tumor–stromal interaction more complex. Conclusions: CAFs are highly chemoresistant. Direct cell–cell contact and high levels of IL-6 correlate with a high chemoresistance. | Christopher C.M.Neumann Ellen von Horschelmann Anja Reutzel-Selke Elisabeth Seidel Igor Maximilian Sauer Johann Pratschke Marcus Bahra Rosa Bianca Schmuck | 2018 | Hepatobiliary & Pancreatic Diseases International2018，17，5： | 2 |
| 6 | Current Concepts on Gastric Carcinoid Tumors显示文摘 | George C. Nikou Theodoros P. Angelopoulos Marcus Bahra | 2012 | Gastroenterology Research and Practice2012，，： | 2 |
| 7 | Patient and tumour biology predict survival beyond the M ilan criteria in liver transplantation for hepatocellular carcinoma显示文摘 | Andreas Andreou Safak Gül Andreas Pascher Wenzel Sch?ning Hussein Al‐Abadi Marcus Bahra Fritz Klein Timm Denecke Benjamin Strücker Gero Puhl Johann Pratschke Daniel Seehofer | 2015 | HPB2015，，2： | 1 |
| 8 | The DNA index is a strong predictive marker in intrahepatic cholangiocarcinoma: the results of a five-year prospective study显示文摘 | Carsten Kamphues Nadine Al-Abadi Angelika Dürr Roberta Bova Frederick Klauschen Albrecht Stenzinger Marcus Bahra Hussein Al-Abadi Peter Neuhaus Daniel Seehofer | 2014 | Surgery Today2014，，7： | 1 |
| 9 | Outcome after liver re‐transplantation in patients with recurrent chronic hepatitis C显示文摘 | Marcus Bahra Ulf Peter Neumann Dietmar Jacob Thomas Berg Ruth Neuhaus Jan Michael Langrehr Peter Neuhaus | 2007 | Transplant International2007，，9： | 1 |
| 10 | Distal Pancreatectomy With En Bloc Resection of the Celiac Trunk for Extended Pancreatic Tumor Disease: An Interdisciplinary Approach显示文摘 | Timm Denecke Andreas Andreou Petr Podrabsky Christian Grieser Peter Warnick Marcus Bahra Fritz Klein Bernd Hamm Peter Neuhaus Matthias Glanemann | 2011 | CardioVascular and Interventional Radiology2011，，： | 1 |
| 11 | Postoperative Complications Deteriorate Long-Term Outcome in Pancreatic Cancer Patients显示文摘 | Carsten Kamphues Roberta Bova Dorothee Schricke Michael Hippler-Benscheidt Frederick Klauschen Albrecht Stenzinger Daniel Seehofer Matthias Glanemann Peter Neuhaus Marcus Bahra | 2012 | Annals of Surgical Oncology2012，，： | 1 |
| 12 | Cytoreductive Surgery for Pancreatic Cancer Improves Overall Outcome of Gemcitabine-Based Chemotherapy显示文摘 | Marcus Bahra Johann Pratschke Fritz Klein Peter Neuhaus Sabine Boas-Knoop Gero Puhl Timm Denecke Joyce R. Pullankavumkal Marianne Sinn Hanno Riess Uwe Pelzer | 2015 | Pancreas2015，，： | 1 |
| 13 | Treatment of Patients with Recurrent Hepatitis C after Liver Transplantation with Peginterferon Alfa-2B Plus Ribavirin显示文摘 | Ulf Neumann Gero Puhl Marcus Bahra Thomas Berg Jan Michael Langrehr Ruth Neuhaus Peter Neuhaus | 2006 | Transplantation2006，，1： | 1 |
| 14 | Long-term results of liver resection for hepatocellular carcinoma in noncirrhotic liver显示文摘 | Wladimir Faber Siamak Sharafi Martin Stockmann Timm Denecke Bruno Sinn Gero Puhl Marcus Bahra Maciej B. Malinowski Peter Neuhaus Daniel Seehofer | 2012 | Surgery2012，，： | 1 |
| 15 | Antiviral Treatment of Patients with Recurrent Hepatitis C After Liver Transplantation with Pegylated Interferon显示文摘 | Sven C. Schmidt Marcus Bahra Sandra Bayraktar Thomas Berg Maximilian Schmeding Johann Pratschke Peter Neuhaus Ulf Neumann | 2010 | Digestive Diseases and Sciences2010，，7： | 1 |
| 16 | Metastatic Pancreatic Cancer: Are We Making Progress in Treatment?显示文摘 | Joanne Chiu Thomas Yau Marcus Bahra | 2012 | Gastroenterology Research and Practice2012，，： | 1 |
| 17 | Mycophenolate Mofetil Monotherapy in Liver Transplantation: 5-Year Follow-Up of a Prospective Randomized Trial显示文摘 | Maximilian Schmeding Anja Kiessling Ruth Neuhaus Christoph Heidenhain Marcus Bahra Peter Neuhaus Ulf P. Neumann | 2011 | Transplantation2011，，8： | 1 |
| 18 | Role of IL28B Polymorphism in the Development of Hepatitis C Virus-Induced Hepatocellular Carcinoma, Graft Fibrosis, and Posttransplant Antiviral Therapy显示文摘 | Dennis Eurich Sabine Boas-Knoop Marcus Bahra Ruth Neuhaus Rajan Somasundaram Peter Neuhaus Ulf Neumann Daniel Seehofer | 2012 | Transplantation2012，，6： | 1 |
| 19 | Prolyl Hydroxylase Domain 2 Protein Is a Strong Prognostic Marker in Human Gastric Cancer显示文摘 | Kamphues Carsten Wittschieber Daniel Klauschen Frederick Kasajima Atsuko Dietel Manfred Schmidt Sven-christian Glanemann Matthias Bahra Marcus Neuhaus Peter Weichert Wilko Stenzinger Albrecht | 2012 | Pathobiology2012，，： | 1 |
| 20 | Fibrosis progression after liver transplantation in patients with recurrent hepatitis C显示文摘 | Ulf P. Neumann Thomas Berg Marcus Bahra Daniel Seehofer Jan M. Langrehr Ruth Neuhaus Cornelia Radke Peter Neuhaus | 2004 | Journal of Hepatology2004，，5： | 1 |
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