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| 1 | Establishment of a dmp based maternal haploid induction system for polyploid Brassica napus and Nicotiana tabacum显示文摘Doubled haploid(DH) technology is used to obtain homozygous lines in a single generation, a technique that significantly accelerates the crop breeding trajectory. Traditionally, in vitro culture is used to generate DHs, but this technique is limited by species and genotype recalcitrance. In vivo haploid induction(HI) through seed is widely and efficiently used in maize and was recently extended to several other crops. Here we show that in vivo HI can be triggered by mutation of DMP maternal haploid inducer genes in allopolyploid(allotetraploid) Brassica napus and Nicotiana tabacum. We developed a pipeline for selection of DMP orthologs for clustered regularly interspaced palindromic repeats mutagenesis and demonstrated average amphihaploid induction rates of2.4% and 1.2% in multiple B. napus and N. tabacum genotypes, respectively. These results further confirmed the HI ability of DMP gene in polyploid dicot crops. The DMP-HI system offers a novel DH technology to facilitate breeding in these crops. The success of this approach and the conservation of DMP genes in dicots suggest the broad applicability of this technique in other dicot crops. | Yu Zhong Yuwen Wang Baojian Chen Jinchu Liu Dong Wang Mengran Li Xiaolong Qi Chenxu Liu Kim Boutilier Shaojiang Chen | 2022 | Journal of Integrative Plant Biology2022，64，6： | 4 |
| 2 | Fault Diagnosis of Analog Circuit Based on PSO and BP Neural Network显示文摘In order to improve the speed and accuracy of analog circuit fault diagnosis,using Back Propagation Neural Network(BPNN),a new method is proposed based on Particle Swarm Optimization(PSO)to adjust weights of BP neural network.The model can not only overcome the limitations of the slow convergence and the local extreme values by basic BP algorithm,but also improve the learning ability and generalization ability with a higher precision.The response signals of analog circuit is preprocessed by Wavelet Packet Transform(WPT)as the fault feature.The simulation result shows that the proposed method has higher diagnostic accuracy and faster convergence speed,which is effective for fault location. | JI Mengran CHEN Gang YANG Qing ZHANG Jinge | 2014 | 沈阳理工大学学报2014，33，5： | 1 |
| 3 | A lipid-based LMP2-mRNA vaccine to treat nasopharyngeal carcinoma显示文摘Nasopharyngeal carcinoma(NPC)is a serious and highly invasive epithelial malignancy that is closely associated with Epstein‒Barr virus(EBV).Due to the lack of therapeutic vaccines for NPC,we selected EBV latent membrane protein 2(LMP2)as a preferable targeting antigen to develop a lipid-based LMP2-mRNA(mLMP2)vaccine.Full-length mLMP2 expressing LMP2 was first synthesized using an in vitro transcription method and then encapsulated into(2,3-dioleacyl propyl)trimethylammonium chloride(DOTAP)-based cationic liposomes to obtain the mRNA vaccine(LPX-mLMP2).The cell assays showed that the antigenpresenting cells were capable of highly efficient uptake of LPX-mLMP2 and expression of LMP2.LMP2 could subsequently be presented to form the peptide-major histocompatibility complex(pMHC).Furthermore,LPX-mLMP2 could accumulate in the spleen,express antigens,promote the maturation of dendritic cells and stimulate antigen-specific T-cell responses in vivo.It dramatically inhibited the tumor growth of the LMP2-expressing tumor model after three doses of vaccination.Additionally,the proliferation of antigen-specific T cells in the tumor site made a good sign for the promise of mRNA vaccines in virus-induced cancer.Overall,we provided a newly developed antigen-encoding mRNA vaccine with advantages against NPC.We also demonstrated that mRNA vaccines are attractive candidates for cancer immunotherapy. | Mengran Guo Xing Duan Xingchen Peng Zhaohui Jin Hai Huang Wen Xiao Qian Zheng Yongqi Deng Na Fan Kepan Chen Xiangrong Song | 2023 | Nano Research2023，16，4： | 1 |
| 4 | A novel aluminum-carbon nanotubes nanocomposite with doubled strength and preserved electrical conductivity显示文摘Enhancing the mechanical strength of highly conductive pure metals usually causes significant reduction in their electrical conductivity.For example,introducing phase/matrix interfaces or more grain boundaries,are common and effective methods to strengthen metals.But it simultaneously increases the electron scattering at the interface,thus reducing the electrical conductivity.In this study,we demonstrate that pure aluminum(Al)/carbon nanotubes(CNTs)nanocomposites prepared by friction stir processing have successfully broken through these limitations.The yield strength and tensile strength of Al/CNTs nanocomposites have improved by 104.7%and 51.8%compared to pure Al,while the electrical conductivity remained comparable to that of pure Al.To explore the potential mechanisms,the interface between CNTs and Al was examined and characterized by transmission electron microscopy(TEM)and Raman spectroscopy.Little interfacial reaction compounds were present and no visible physical gaps were observed at CNTs and Al interfaces.We defined it as a clean and tightly bonded interface.Although the quantity of phase interface has increased,the electrical conductivity of the nanocomposite remains approximately unchanged.We attribute the preserved electrical conductivity to the clean and tightly bonded CNTs/Al interface in the nanocomposite. | Shuai Zhang Gaoqiang Chen Timing Qu Jinquan Wei Yufan Yan Qu Liu Mengran Zhou Gong Zhang Zhaoxia Zhou Huan Gao Dawei Yao Yuanwang Zhang Qingyu Shi Hua Zhang | 2021 | Nano Research2021，14，8： | 1 |
| 5 | Sodium alginate coating simultaneously increases the biosafety and immunotherapeutic activity of the cationic mRNA nanovaccine显示文摘The extraordinary advantages associated with mRNA vaccines,including their high efficiency,relatively low severity of side effects,and ease of manufacture,have enabled them to be a promising immunotherapy approach against various infectious diseases and cancers.Nevertheless,most mRNA delivery carriers have many disadvantages,such as high toxicity,poor biocompatibility,and low efficiency in vivo,which have hindered the widespread use of mRNA vaccines.To further characterize and solve these problems and develop a new type of safe and efficient mRNA delivery carrier,a negatively charged SA@DOTAP-mRNA nanovaccine was prepared in this study by coating DOTAP-mRNA with the natural anionic polymer sodium alginate(SA).Intriguingly,the transfection efficiency of SA@DOTAP-mRNA was significantly higher than that of DOTAP-mRNA,which was not due to the increase in cellular uptake but was associated with changes in the endocytosis pathway and the strong lysosome escape ability of SA@DOTAP-mRNA.In addition,we found that SA significantly increased the expression of LUC-mRNA in mice and achieved certain spleen targeting.Finally,we confirmed that SA@DOTAP-mRNA had a stronger antigen-presenting ability in E.G7-OVA tumor-bearing mice,dramatically inducing the proliferation of OVA-specific CLTs and ameliorating the antitumor effect.Therefore,we firmly believe that the coating strategy applied to cationic liposome/mRNA complexes is of potential research value in the field of mRNA delivery and has promising clinical application prospects. | Xing Duan Yi Zhang Mengran Guo Na Fan Kepan Chen Shugang Qin Wen Xiao Qian Zheng Hai Huang Xiawei Wei Yuquan Wei Xiangrong Song | 2023 | Acta Pharmaceutica Sinica B2023，13，3： | 1 |
| 6 | Melphalan-loaded methoxy poly(ethylene glycol)-poly(D,L-lactide)copolymer nanomicelles in the treatment of multiple myeloma显示文摘Multiple myeloma(MM)is the second most common hematological tumor characterized by the proliferation of monoclonal plasma cells.Melphalan(MEL)is commonly used in the treatment of MM and is especially essential for patients undergoing autologous stem cell transplantation(ASCT).Although many drugs for MM have been developed in recent years,chemotherapy followed by ASCT remains the optimal option.Melphalan,the backbone of the conditioning regimen,brings severe toxicities at a high dose.Nanodrug delivery systems enable drugs to be highly effective and have low toxicity.In this study,methoxy poly(ethylene glycol)-poly(D,L-lactide)copolymer(MPEG-PDLLA)was chosen to encapsulate melphalan,and the characteristics,effectiveness,and safety of MEL/MPEG-PDLLA in vitro and in vivo were investigated.MEL/MPEG-PDLLA showed slow release and was easily engulfed by MM cells despite a result of the antitumor assay comparable to that of free melphalan in vitro.The in vivo results showed that MEL/MPEG-PDLLA could significantly alleviate tumor burden and prolong survival time without increasing the toxicity to vital organs.In addition,MEL/MPEG-PDLLA could significantly reduce the damage to the intestinal mucosa caused by melphalan.In conclusion,MEL/MPEG-PDLLA shows improved antitumor activity and has the potential to alleviate pains of MM patients undergoing ASCT. | Yingying Chen Qiang Zeng Bingyang Chu Zhigang Liu Xue Wei Mengran Chen Peipei Yang Minghai Tang Ting Niu Yongqian Jia Ying Qu Zhiyong Qian | 2023 | Chinese Chemical Letters2023，34，9： | 0 |
| 7 | Geology, environment, and life in the deepest part of the world’s oceans显示文摘The hadal zone,mostly comprising of deep trenches and constituting of the deepest part of the world’s oceans,represents the least explored habitat but one of the last frontiers on our planet.The present scientific understanding of the hadal environment is still relatively rudimentary,particularly in comparison with that of shallower marine environments.In the last 30 years,continuous efforts have been launched in deepening our knowledge regarding the ecology of the hadal trench.However,the geological and environmental processes that potentially affect the sedimentary,geochemical and biological processes in hadal trenches have received less attention.Here,we review recent advances in the geology,biology,and environment of hadal trenches and offer a perspective of the hadal science involved therein.For the first time,we release highdefinition images taken by a new full-ocean-depth manned submersible Fendouzhe that reveal novel species with an unexpectedly high density,outcrops of mantle and basaltic rocks,and anthropogenic pollutants at the deepest point of the world’s ocean.We advocate that the hydration of the hadal lithosphere is a driving force that influences a variety of sedimentary,geochemical,and biological processes in the hadal trench.Hadal lithosphere might host the Earth’s deepest subsurface microbial ecosystem.Future research,combined with technological advances and international cooperation,should focus on establishing the intrinsic linkage of the geology,biology,and environment of the hadal trenches. | Mengran Du Xiaotong Peng Haibin Zhang Cong Ye Shamik Dasgupta Jiwei Li Jiangtao Li Shuangquan Liu Hengchao Xu Chuanxu Chen Hongmei Jing Hongzhou Xu Jun Liu Shunping He Lisheng He Shanya Cai Shun Chen Kaiwen Ta | 2021 | The Innovation2021，2，2： | 0 |
| 8 | π-Extension and chlorination of non-fullerene acceptors enable more readily processable and sustainable high-performance organic solar cells显示文摘Organic solar cells(OSCs)processed without halogenated solvents and complex treatments are essential for future commercialization.Herein,we report three novel small molecule acceptors(NFAs)consisting of a Y6-like core but withπ-extended naphthalene with progressively more chlorinated end-capping groups and a longer branched chain on the Nitrogen atom.These NFAs exhibit good solubilities in nonchlorinated organic solvents,broad optical absorptions,closeπ-πstacking distances(3.63–3.84A),and high electron mobilities(~10^(-3)cm^(2)V^(-1)s^(-1)).The o-xylene processed and as-cast binary devices using PM6 as the donor polymer exhibit a PCE increasing upon progressive chlorination of the naphthalene end-capping group from 8.93%for YN to 14.38%for YN-Cl to 15.00%for YN-2Cl.Furthermore similarly processed ternary OSCs were fabricated by employing YN-Cl and YN-2Cl as the third component of PM6:CH1007 blends(PCE=15.75%).Compared to all binary devices,the ternary PM6:CH1007:YN-Cl(1:1:0.2)and PM6:CH1007:YN-2Cl(1:1:0.2)cells exhibit significantly improved PCEs of 16.49%and15.88%,respectively,which are among the highest values reported to date for non-halogenated solvent processed OSCs without using any additives and blend post-deposition treatments. | Ning Su Jianhua Chen Mengran Peng Guoping Li Robert M.Pankow Ding Zheng Junqiao Ding Antonio Facchetti Tobin J.Marks | 2023 | Journal of Energy Chemistry2023，，4： | 0 |
| 9 | Annotating TSSs in Multiple Cell Types Based on DNA Sequence and RNA-seq Data via DeeReCT-TSS显示文摘The accurate annotation of transcription start sites(TSSs)and their usage are critical for the mechanistic understanding of gene regulation in different biological contexts.To fulfill this,specific high-throughput experimental technologies have been developed to capture TSSs in a genome-wide manner,and various computational tools have also been developed for in silico prediction of TSSs solely based on genomic sequences.Most of these computational tools cast the problem as a binary classification task on a balanced dataset,thus resulting in drastic false positive predictions when applied on the genome scale.Here,we present Dee Re CT-TSS,a deep learningbased method that is capable of identifying TSSs across the whole genome based on both DNA sequence and conventional RNA sequencing data.We show that by effectively incorporating these two sources of information,Dee Re CT-TSS significantly outperforms other solely sequence-based methods on the precise annotation of TSSs used in different cell types.Furthermore,we develop a meta-learning-based extension for simultaneous TSS annotations on 10 cell types,which enables the identification of cell type-specific TSSs.Finally,we demonstrate the high precision of DeeReCT-TSS on two independent datasets by correlating our predicted TSSs with experimentally defined TSS chromatin states.The source code for Dee Re CT-TSS is available at https://github.-com/Joshua Chou2018/Dee Re CT-TSS_release and https://ngdc.cncb.ac.cn/biocode/tools/BT007316. | Juexiao Zhou Bin Zhang Haoyang Li Longxi Zhou Zhongxiao Li Yongkang Long Wenkai Han Mengran Wang Huanhuan Cui Jingjing Li Wei Chen Xin Gao | 2022 | Genomics, Proteomics & Bioinformatics2022，20，5： | 0 |
| 10 | Effect of Dandelion(Taraxacum mongolicum Hand.-Mazz.)Intercropping with Different Plant Spacing on Blight and Growth of Pepper(Capsicum annuum L.)显示文摘Intercropping of crops that can secrete bacteriostatic active substances can not only inhibit the occurrence of disease but also have an important effect on plant growth.However,the effects of dandelion intercropping on pepper blight control and pepper growth remain unclear.In this study,the control effect of dandelion on pepper blight was studied by inoculating the pepper leaves with Phytophthora infestans,and it also discusses the correlation of the occurrence of pepper epidemic disease with the pepper canopy environment,soil environment,pepper photo-synthesis,and yield index.The results showed that best plant distance for dandelion intercropping was 20 cm(P20),and the control effect reached 43.31%.As compared to the CK,SOD enzyme,POD enzyme,and PAL enzyme were significantly up-regulated during the growth of pepper;chlorophyll content in pepper leaves was significantly increased;photosynthetic characteristics were significantly increased;stem diameter and yield of crop pepper were effectively improved;and the quality of the pepper product was better,but intercropping dandelion resulted in a significant decrease of nutrients in the soil environment of pepper,so a reasonable intercropping distance was needed.The correlation analysis shows that the incidence of pepper blight(A)was significantly positively correlated with soil temperature(Q),intercellular carbon dioxide(L),and canopy air temperature(O).The incidence of capsicum blight(A)was significantly negatively correlated with chlorophyll content(F),net photo-synthetic rate(K),stomatal conductance(M),ww rate(N),soil sucrase activity(W),vitamin C(AB),and leaf PAL enzyme(J).Finally,it was deduced that intercropping dandelion could effectively control the occurrence of pepper blight while also demonstrating a complex interaction with the pepper growing environment. | Peixin Li Hanbing Liu Yingtong Chen Xin Zhang Ning Cao Ying Sun Meimei Jia Mengran Wu Xuejiao Tong Xinmei Jiang Xihong Yu Yao Cheng | 2023 | Phyton-International Journal of Experimental Botany2023，92，8： | 0 |
| 11 | Simultaneous enhancement of mechanical properties and corrosion resistance of as-cast Mg-5Zn via microstructural modification by friction stir processing显示文摘Magnesium alloys are ideal lightweight materials;however,their applications are extremely limited due to their low strength,poor ductility,and weak corrosion resistance.In the present study,a friction stir processing(FSP)treatment was employed to optimize the mechanical properties and corrosion resistance of an as-cast Mg-5Zn alloy.The average grain size of the Mg-5Zn alloy was refined from 133.8μm to1.3μm as a result of FSP.Along different directions,FSP exhibited the enhancement effects on different mechanical properties.Furthermore,according to the potentiodynamic polarization results,the corrosion current density at the free-corrosion potential of the FSPed sample,was 4.1×10^(-6)A/cm^(2)in 3.5 wt.%Na Cl aqueous solution,which was significantly lower than that of the as-cast sample.Electrochemical impedance spectroscopy revealed that the polarization impedance,Rp,of the FSPed sample was 1534Ω/cm^(2)in 3.5 wt.%NaCl aqueous solution,which was 71.4%greater than that of the as-cast sample.The corrosion morphology of the FSPed sample in 3.5 wt.%NaCl aqueous solution exhibited largely uniform corrosion,rather than severe localized corrosion characteristics,which further reduced the corrosion depth on the basis of reducing the corrosion current density.The results presented herein indicate that FSP is a viable technique for simultaneously improving the mechanical properties and corrosion resistance of the as-cast Mg-5Zn alloy. | Fei Long Gaoqiang Chen Mengran Zhou Qingyu Shi Qu Liu | 2023 | Journal of Magnesium and Alloys2023，11，6： | 0 |
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