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| 1 | Pea3 expression promotes the invasive and metastatic potential of colorectal carcinoma显示文摘AIM:To investigate the function of Pea3 in colorectal carcinoma(CRC)invasion and metastatic potential.METHODS:The expression of Pea3 during clinical progression of human CRC was investigated using Oncomine Research Edition.To assay Pea3 expression in established CRC cell lines,we performed western blotting of cell lysates.We employed sh RNA-mediated knockdown of Pea3 in HCT116(HCT)and LS174T CRC cells which was confirmed by real-time quantitative PCR(q PCR)and western blotting.Transwell invasion assays,MTS proliferation assays,anoikis assays,and fluorometric matrix metalloprotease(MMP)assays wereperformed to determine the effects of Pea3 knockdown on invasion,proliferation,anoikis and MMP activity in CRC cells in vitro.Alterations in epithelial-mesenchymal transition(EMT)and matrix metalloprotease(MMP)m RNA levels were determined by q PCR.CRC cells were injected into the flanks of nude mice to generate xenografts and tumor growth monitored with serial calliper measurements.To assay metastatic potential,CRC cells were injected into the spleen of nude mice,and histological analysis performed on the livers 21 d later.RESULTS:We demonstrated that reduction of Pea3expression in CRC cells significantly impaired their invasive capacity(HCT.sh Pea3,0.28±0.04 fold,P<0.01;LS.sh Pea3,0.15±0.04 fold;SW.sh Pea3,0.23±0.03,P<0.01),reduced anoikis resistance(HCT.sh Pea3 75.4%±1.9%viable cells vs HCT.sh Ctrl 88.6%±0.6%viable cells,P<0.01;LS.sh Pea3 71.7%±0.5%viable cells vs LS.Ctrl 89.6%±0.3%viable cells,P<0.005,but had no effect on proliferation(HCT.sh Ctrl AUC 5098±123 vs HCT.sh Pea3 5689±151,P<0.05;LS.sh Ctrl AUC 5600±324.1 vs LS.sh Pea3 6423±400,P<0.05).In vivo,HCT.sh Pea3 and HCT.sh Ctrl tumour xenografts grew at a similar rate(HCT.sh Pea3 2.64±0.82 fold vs HCT.sh Ctrl 2.88±0.80 fold,P>0.05).In keeping with a pro-metastatic function for Pea3 in CRC,several EMT markers and MMPs were downregulated in sh Pea3-expressing cells,suggesting that Pea3 may exert its effects through these processes.A reduction in overall MMP activity was observed in HCT.sh Pea3 cells compared to their control counterparts(HCT.sh Pea30.61±0.04 fold,P<0.005).This translated in vivo to the complete absence of metastases in the livers of mice that were grafted with CRC cells lacking Pea3.Conversely,CRC cells expressing Pea3 formed liver metastases in all mice.CONCLUSION:Our study implicates Pea3 as a mediator of metastases,and provides a biological rationale for the adverse prognosis associated with elevated Pea3 expression in human CRC. | Aruz Mesci Samira Taeb Xiaoyong Huang Rishi Jairath Darshan Sivaloganathan Stanley K Liu | 2014 | World Journal of Gastroenterology2014，20，46： | 7 |
| 2 | Three-dimensional bioprinted glioblastoma microenvironments model cellular dependencies and immune interactions显示文摘Brain tumors are dynamic complex ecosystems with multiple cell types.To model the brain tumor microenvironment in a reproducible and scalable system,we developed a rapid three-dimensional(3D)bioprinting method to construct clinically relevant biomimetic tissue models.In recurrent glioblastoma,macrophages/microglia prominently contribute to the tumor mass.To parse the function of macrophages in 3D,we compared the growth of glioblastoma stem cells(GSCs)alone or with astrocytes and neural precursor cells in a hyaluronic acid-rich hydrogel,with or without macrophage.Bioprinted constructs integrating macrophage recapitulate patient-derived transcriptional profiles predictive of patient survival,maintenance of stemness,invasion,and drug resistance.Whole-genome CRISPR screening with bioprinted complex systems identified unique molecular dependencies in GSCs,relative to sphere culture.Multicellular bioprinted models serve as a scalable and physiologic platform to interrogate drug sensitivity,cellular crosstalk,invasion,context-specific functional dependencies,as well as immunologic interactions in a species-matched neural environment. | Min Tang Qi Xie Ryan C.Gimple Zheng Zhong Trevor Tam Jing Tian Reilly L.Kidwell Qiulian Wu Briana C.Prager Zhixin Qiu Aaron Yu Zhe Zhu Pinar Mesci Hui Jing Jacob Schimelman Pengrui Wang Derrick Lee Michael H.Lorenzini Deobrat Dixit Linjie Zhao Shruti B.hargava Tyler E.Miller Xueyi Wan Jing Tang Bingjie Sun Benjamin F.Cravatt Alysson R.Muotri Shaochen Chen Jeremy N.Rich | 2020 | Cell Research2020，30，10： | 6 |
| 3 | Assessment of endothelial function in patients with nonalcoholic fatty liver disease显示文摘 | Yasar Colak Ebubekir Senates Atakan Yesil Yusuf Yilmaz Oguzhan Ozturk Levent Doganay Ender Coskunpinar Ozlem Timirci Kahraman Banu Mesci Celal Ulasoglu Ilyas Tuncer | 2013 | Endocrine2013，，1： | 2 |
| 4 | Visual acuity and contrast sensitivity function after accommodative and multifocal intraocular lens implantation显示文摘 | Mesci C Erbil H Ozdoker L | 2010 | Eur J Ophthalmol2010，20，1： | 1 |
| 5 | Use of pistachio shells as an adsorbent for the removal of zinc ( II ) ion 显示文摘 | Turan N G Mesci B | 2011 | Clean-Soil Air Water2011，39，5： | 1 |
| 6 | The use of artificial neural networks (ANN) for modeling of adsorption of Cu(II) from industrial leachate by pumice 显示文摘 | N Gamze Turana Basak Mesci Okan Ozgonenel | 2011 | Chemical Engineering Journal2011，171，： | 1 |
| 7 | The effects of neuromuscular e- lectrical stimulation on clinical improvement in hemiplegic lower extremi- ty rehabilitation in chronic stroke : a single-blind, randomised, controlled trial显示文摘 | Mesci N Ozdemir F Kabayel DD | 2009 | Disabil Rehabil2009，31，24： | 1 |
| 8 | Visual acuity and contrast sen-sitivity function after accommodative and multifocal intraocular lensimplantation显示文摘 | Mesci C Erbil H Ozdoker L | 2010 | Eur J Ophthalmol2010，20，1： | 1 |
| 9 | Association of tumour necrosis factor-alpha -308 G/A polymorphism with primary open-angle glancoma显示文摘 | Bozkurt B Mesci L Irkec M | 2012 | Clin Experiment Ophthalmol2012，40，4： | 1 |
| 10 | Deep selerectomy augmented with combination of absorbable biosynthetic sodium hyahronateseleral implantand mitomycin C or with mitomycin C versus trabeculectomy:long term results显示文摘 | Mesci C Eril HH Karakurt Y | 2012 | Clin Exp Ophthalmol2012，40，4： | 1 |
| 11 | Digital evaluation of the mus- cle functions of the lower extremities among inguinal hernia patients treated using three different surgical techniques:a prospective random- ized study显示文摘 | Mesci A Korkmaz B Dinckan A | 2012 | Surg Today2012，42，4： | 1 |
| 12 | Visual acuity and contrast sensitivity function after accommodative and multifocal intraocular lens implantation显示文摘 | Mesci C Erbil H Ozdoker L | 2010 | Eur J Ophthalmol2010，20，1： | 1 |
| 13 | Visual acuity and contrast sensitivity function after accommodative and multifocal intraocuiar lens implantation显示文摘 | Mesci C Erbil H Ozdoker L Karakurt Y Bilge AD | | 0，，01： | 1 |
| 14 | Visual acuity and contrast sensitivity function after accommodative and multifocal intraocular lens implantation显示文摘 | Mesci C Erbil H Ozdoker L | 2009 | Eur J Ophthalmol2009，20，1： | 1 |
| 15 | Visual acuity and contrast sensitivity function after accommodative and multifocal intraocular lens implantation显示文摘 | Mesci C Erbil H Ozdoker L | | 0，，： | 1 |
| 16 | Cytomegalovirus evasion of innate immunity by subversion of the NKR-PIB: Clr-b missing- self axis显示文摘 | Voigt S Mesci A Ettinger J | 2007 | Immunity2007，26，5： | 1 |
| 17 | Differences in contrast sensiti(v)ity between monofocal,multifocal and accommodating intrao(e)ular lenses: long-term results显示文摘 | Mesci (C) Erbil HH Olgun A | | 0，，： | 1 |
| 18 | Cytomegalovirus evasion of innate immunity by subversion of the NKR-P1B: Clr-b missing-self axis显示文摘 | Voigt S Mesci A Ettinger J | 2007 | Immunity2007，26，5： | 1 |
| 19 | Digital evalua- tion of the muscle functions of the lower extremities among in- guinal hernia patients treated using three different surgical techniques : a prospective randomized study 显示文摘 | MESCI A KORKMAZ B DINCKAN A | 2012 | Surg Today2012，42，4： | 1 |
| 20 | Investigation of the Possibility of Copper Removal from Industrial Leachate by Raw and Calcined Phosphogypsum:D‐Optimal and Taguchi Designs显示文摘 | MESCI B TURAN N G OZGONENEL O | 2012 | Clean–Soil Air Water2012，40，8： | 1 |
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