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| 1 | Paeonol inhibits tumor growth in gastric cancer in vitro and in vivo显示文摘AIM:To investigate the anti-tumor effects of paeonol in gastric cancer cell proliferation and apoptosis in vitro and in vivo.METHODS:Murine gastric cancer cell line mouse forestomach carcinoma(MFC) or human gastric cancer cell line SGC-7901 was cultured in the presence or absence of paeonol.Cell proliferation was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay,and cell cycle and apoptosis by flow cytometry and TUNEL staining.Tumor growth after subcutaneous implantation of MFC cells in mice was monitored,and the effects of treatment with paeonol were determined.RESULTS:In vitro,paeonol caused dose-dependent inhibition on cell proliferation and induced apoptosis.Cell cycle analysis revealed a decreased proportion of cells in G0/G1 phase,with arrest at S.Paeonol treatment in gastric cancer cell line MFC and SGC-790 cells significantly reduced the expression of Bcl-2 and increased the expression of Bax in a concentration-related manner.Administration of paeonol to MFC tumor-bearing mice significantly lowered the tumor growth and caused tumor regression.CONCLUSION:Paeonol has signif icantly growth-inhibitory and apoptosis-inducing effects in gastric cancer cells both in vitro and in vivo. | Na Li Lu-Lu Fan Guo-Ping Sun Xin-An Wan Zhang-Gui Wang Qiang Wu Hua Wang | 2010 | World Journal of Gastroenterology2010，16，35： | 27 |
| 2 | Brucine Inhibits Bone Metastasis of Breast Cancer Cells by Suppressing Jagged1/Notch1 Signaling Pathways显示文摘Objective: To examine the effects of brucine on the invasion, migration and bone resorption of receptor activator of nuclear factor-kappa B ligand(RANKL)-induced osteoclastogenesis. Methods: The osteoclastogenesis model was builded by co-culturing human breast tumor MDA-MB-231 and mouse RAW264.7 macrophages cells. RANKL(50 ng/m L) and macrophage-colony stimulating factor(50 ng/m L) were added to this system, followed by treatment with brucine(0.02, 0.04 and 0.08 mmol/L), or 10 μmol/L zoledronic acid as positive control. The migration and bone resorption were measured by transwell assay and in vitro bone resorption assay. The protein expressions of Jagged1 and Notch1 were investigated by Western blot. The expressions of transforming growth factor-β1(TGF-β1), nuclear factor-kappa B(NF-κB) and Hes1 were determined by enzyme-linked immunosorbent assay. Results: Compared with the model group, brucine led to a dose-dependent decrease on migration of MDA-MB-231 cells, inhibited RANKL-induced osteoclastogenesis and bone resorption of RAW264.7 cells(P <0.01). Furthermore, brucine decreased the protein levels of Jagged1 and Notch1 in MDA-MB-231 cells and RAW264.7 cells co-cultured system as well as the expressions of TGF-β1, NF-κB and Hes1(P<0.05 or P<0.01). Conclusion: Brucine may inhibit osteoclastogenesis by suppressing Jagged1/Notch1 signaling pathways. | HU Ke-fei KONG Xiang-ying ZHONG Mi-cun WAN Hong-ye LIN Na PEI Xiao-hua | 2017 | Chinese Journal of Integrative Medicine2017，23，2： | 17 |
| 3 | Piglet gut microbial shifts early in life:causes and effects显示文摘The gut microbiome has long been known to play fundamentally important roles in the animal health and the well-being of its host. As such, the establishment and maintenance of a beneficial gut microbiota early in life is crucial in pigs, since early gut colonizers are pivotal in the establishment of permanent microbial community structures affecting the health and growth performance of pigs later in life. Emphasizing this importance of early gut colonizers, it is critical to understand the factors impacting the establishment of the piglet gut microbiome at weaning. Factors include, among others, diet, in-feed antibiotics, probiotics and prebiotic administration. The impact of these factors on establishment of the gut microbiome of piglets at weaning includes effects on piglet gut microbial diversity, structure, and succession. In this review, we thoroughly reviewed the most recent findings on the piglet gut microbiome shifts as influenced by weaning, and how these microbiome changes brought about by various factors that have been shown to affect the development of microbiota in piglets. This review will provide a general overview of recent studies that can help to facilitate the design of new strategies to modulate the gut microbiome in order to enhance gastrointestinal health, growth performance and well-being of piglets. | Robin B.Guevarra Jun Hyung Lee Sun Hee Lee Min-Jae Seok Doo Wan Kim Bit Na Kang Timothy J.Johnson Richard E.Isaacson Hyeun Bum Kim | 2019 | Journal of Animal Science and Biotechnology2019，10，3： | 13 |
| 4 | Selenium Supplementation Alleviates Autoimmune Thyroiditis by Regulating Expression of Th1/Th2 Cytokines显示文摘Selenium (Se) is an essential trace element.Autoimmune thyroid diseases (AITD) are destructive inflammatory or anti-receptor autoimmune diseases characterized by reactivity to self-thyroid antigens.However,the effects of Se on the cytokines in AITD are still unclear.So we researched the role of Selenium (Se) and Th1/Th2 cytokine productions in the pathogenesis of autoimmune thyroid diseases (AITD).The data shown that high Se intake reduced the autoantibody titers and alleviated the | TAN Long SANG Zhong Na SHEN Jun WU Yun Tang YAO Zhai Xiao ZHANG Jin Xiang ZHAO Na ZHANG Wan Qi | 2013 | Biomedical and Environmental Sciences2013，26，11： | 10 |
| 5 | Biomarkers enhance the long-term predictive ability of the KAMIR risk score in Chinese patients with ST-elevation myocardial infarction显示文摘Background:The Global Registry of Acute Coronary Events (GRACE) score is recommended by current ST-elevation myocardial infarction (STEMI) guidelines.But it has inherent defects.The present study aimed to investigate the more compatible risk stratification for Chinese patients with STEMI and to determine whether the addition of biomarkers to the Korea Acute Myocardial Infarction Registry (KAMIR) score could enhance its predictive value for long-term outcomes.Methods:A total of 1093 consecutive STEMI patients were included and followed up 48.2 months.Homocysteine,hypersensitive C-reactive protein (hs-CRP),and N-terminal pro-B-type natriuretic peptide (NT-proBNP) were detected.The KAMIR score and the GRACE score were calculated.The performance between the KAMIR and the GRACE was compared.The predictive power of the KAMIR alone and combined with biomarkers were assessed by the receiver-operating characteristic (ROC) curve.Results:The KAMIR demonstrated a better risk stratification and predictive ability than the GRACE (death:AUC = 0.802 vs.0.721,P<0.001;major adverse cardiovascular events (MACE):AUC = 0.683 vs.0.656,P<0.001).It showed that the biomarkers could independently predict death [homocysteine:HR= 1.019 (1.015–1.024),P<0.001;hs-CRP:HR= 1.052 (1.000–1.104),P= 0.018;NT-pro BNP:HR= 1.142 (1.004–1.280),P= 0.021] and MACE [homocysteine:HR= 1.019 (1.015–1.024),P<0.001;hs-CRP:HR= 1.012 (1.003–1.021),P= 0.020;NT-pro BNP:HR= 1.136 (1.104–1.168),P= 0.006].When they were used in combination with the KAMIR,the area under the ROC curve (AUC) significantly increased for death [homocysteine:AUC = 0.802 vs.0.890,Z = 5.982,P<0.001;hs-CRP:AUC = 0.802 vs.0.873,Z= 3.721,P<0.001;NT-pro BNP:AUC= 0.802 vs.0.871,Z = 2.187,P= 0.047;homocysteine,hs-CRP and NT-pro BNP:AUC = 0.802 vs.0.940,Z = 6.177,P<0.001] and MACE [homocysteine:AUC = 0.683 vs.0.771,Z= 6.818,P<0.001;hs-CRP:AUC= 0.683 vs.0.712,Z= 2.022,P= 0.031;NT-pro BNP:AUC= 0.683 vs.0.720,Z= 2.974,P= 0.003;homocysteine,hs-CRP and NT-pro BNP:AUC= 0.683 vs.0.789,Z= 6.900,P<0.001].Conclusion:The KAMIR is better than the GRACE in risk stratification and prognosis prediction in Chinese STEMI patients.A combination of above-mentioned biomarkers can develop a more predominant prediction for long-term outcomes. | Jian-Jun Wang Yan Fan Yan Zhu Jian-Dong Zhang Su-Mei Zhang Zhao-Fei Wan Hong-Ling Su Na Jiang | 2019 | Chinese Medical Journal2019，，1： | 9 |
| 6 | Association of Overlapped and Un-overlapped Comorbidities with COVID-19 Severity and Treatment Outcomes: A Retrospective Cohort Study from Nine Provinces in China显示文摘Objective Several COVID-19 patients have overlapping comorbidities. The independent role of each component contributing to the risk of COVID-19 is unknown, and how some non-cardiometabolic comorbidities affect the risk of COVID-19 remains unclear.Methods A retrospective follow-up design was adopted. A total of 1,160 laboratory-confirmed patients were enrolled from nine provinces in China. Data on comorbidities were obtained from the patients’ medical records. Multivariable logistic regression models were used to estimate the odds ratio(OR) and 95% confidence interval(95% CI) of the associations between comorbidities(cardiometabolic or non-cardiometabolic diseases), clinical severity, and treatment outcomes of COVID-19.Results Overall, 158(13.6%) patients were diagnosed with severe illness and 32(2.7%) had unfavorable outcomes. Hypertension(2.87, 1.30–6.32), type 2 diabetes(T2 DM)(3.57, 2.32–5.49),cardiovascular disease(CVD)(3.78, 1.81–7.89), fatty liver disease(7.53, 1.96–28.96), hyperlipidemia(2.15, 1.26–3.67), other lung diseases(6.00, 3.01–11.96), and electrolyte imbalance(10.40, 3.00–26.10)were independently linked to increased odds of being severely ill. T2 DM(6.07, 2.89–12.75), CVD(8.47,6.03–11.89), and electrolyte imbalance(19.44, 11.47–32.96) were also strong predictors of unfavorable outcomes. Women with comorbidities were more likely to have severe disease on admission(5.46,3.25–9.19), while men with comorbidities were more likely to have unfavorable treatment outcomes(6.58, 1.46–29.64) within two weeks.Conclusion Besides hypertension, diabetes, and CVD, fatty liver disease, hyperlipidemia, other lung diseases, and electrolyte imbalance were independent risk factors for COVID-19 severity and poor treatment outcome. Women with comorbidities were more likely to have severe disease, while men with comorbidities were more likely to have unfavorable treatment outcomes. | MA Yan ZHU Dong Shan CHEN Ren Bo SHI Nan Nan LIU Si Hong FAN Yi Pin WU Gui Hui YANG Pu Ye BAI Jiang Feng CHEN Hong CHEN Li Ying FENG Qiao GUO Tuan Mao HOU Yong HU Gui Fen HU Xiao Mei HU Yun Hong HUANG Jin HUANG Qiu Hua HUANG Shao Zhen JI Liang JIN Hai Hao LEI Xiao LI Chun Yan LI Min Qing LI Qun Tang LI Xian Yong LIU Hong De LIU Jin Ping LIU Zhang MA Yu Ting MAO Ya MO Liu Fen NA Hui WANG Jing Wei SONG Fang Li SUN Sheng WANG Dong Ting WANG Ming Xuan WANG Xiao Yan WANG Yin Zhen WANG Yu Dong WU Wei WU Lan Ping XIAO Yan Hua XIE Hai Jun XU Hong Ming XU Shou Fang XUE Rui Xia YANG Chun YANG Kai Jun YUAN Sheng Li ZHANG Gong Qi ZHANG Jin Bo ZHANG Lin Song ZHAO Shu Sen ZHAO Wan Ying ZHENG Kai ZHOU Ying Chun ZHU Jun Teng ZHU Tian Qing ZHANG Hua Min WANG Yan Ping WANG Yong Yan 无 | 2020 | Biomedical and Environmental Sciences2020，33，12： | 8 |
| 7 | Study of recombinant human interleukin-12 for treatment of complications after radiotherapy for tumor patients显示文摘AIM To evaluate the treatment effects of recombinant human interleukin-12(rh IL-12) on radiotherapy complications, such as severe myelosuppression or pancytopenia, the decline or imbalance of immune function, etc.METHODS The patients received high-dose and short-course precise radiotherapy, such as Cyber knife and image-guided radiotherapy(IGRT), which can cause myelosuppression or pancytopenia and immune function decline within a short time. One-hundred subjects were enrolled in the study, and 50 were randomized to a treatment group which used rh IL-12 and 50 were randomized to a control group which used symptomatic and supportive therapy after radiotherapy. The 50 subjects in the treatment group were further divided into five subgroups and intervenedwith rh IL-12 at a dose of 50, 100, 150, 200 or 250 ng/kg respectively. The dose-effect relationship was observed. RESULTS Rh IL-12 significantly attenuated the decrease of peripheral blood cells in the treatment group, and immune function was improved after treatment. Due to the different radiation doses, there was a fluctuation within 12 h after treatment but mostly showing an increasing trend. As to the clinical manifestations, 2 patients in the 250 ng/kg subgroup showed low fever after administration, 1 patient in the 200 ng/kg subgroup and 2 patients in the 250 ng/kg subgroup showed mild impairment of liver function during the observation period.CONCLUSION Rh IL-12 has effective therapeutic and protective effects on complications following radiotherapy, such as the decline of blood cells, myelosuppression and the decline or imbalance of immune function, which indicated good prospects for development and application. | Na Guo Wen-Qin Wang Xiao-Jing Gong Lei Gao Li-Rong Yang Wei-Na Yu Hong-Yu Shen Ling-Qin Wan Xi-Feng Jia Yi-Shan Wang Yi Zhao | 2017 | World Journal of Clinical Oncology2017，8，2： | 7 |
| 8 | UGT74AF3 enzymes specifically catalyze the glucosylation of 4-hydroxy-2,5-dimethylfuran-3(2H)-one,an important volatile compound in Camellia sinensis显示文摘4-Hydroxy-2,5-dimethylfuran-3(2H)-one(HDMF)is an important odorant in some fruits,and is proposed to play a crucial role in the caramel-like notes of some teas.However,its biosynthesis and metabolism in tea plants are still unknown.Here,HDMF glucoside was unambiguously identified as a native metabolite in tea plants.A novel glucosyltransferase UGT74AF3a and its allelic protein UGT74AF3b specifically catalyzed the glucosylation of HDMF and the commercially important structural homologues 2(or 5)-ethyl-4-hydroxy-5(or 2)-methylfuran-3(2H)-one(EHMF)and 4-hydroxy-5-methylfuran-3(2H)-one(HMF)to their correspondingβ-D-glucosides.Site-directed mutagenesis of UGT74AF3b to introduce a single A456V mutation resulted in improved HDMF and EHMF glucosylation activity and affected the sugar donor preference compared with that of the wild-type control enzyme.The accumulation of HDMF glucoside was consistent with the transcript levels of UGT74AF3 in different tea cultivars.In addition,transient UGT74AF3a overexpression in tobacco significantly increased the HDMF glucoside contents,and downregulation of UGT74AF3 transcripts in tea leaves significantly reduced the concentration of HDMF glucoside compared with the levels in the controls.The identification of HDMF glucoside in the tea plant and the discovery of a novel-specific UDP-glucose:HDMF glucosyltransferase in tea plants provide the foundation for improvement of tea flavor and the biotechnological production of HDMF glucoside. | Yongxian Chen Xiangyang Guo Ting Gao Na Zhang Xiaochun Wan Wilfried Schwab Chuankui Song | 2020 | Horticulture Research2020，7，1： | 6 |
| 9 | Molecular characterization of three Hsp90 from Pieris and expression patterns in response to cold and thermal stress in summer and winter diapause of Pieris melete显示文摘热吃惊蛋白质(Hsps ) 在昆虫被连接了到压力和冬季 diapause,但是他们是否是夏天 diapause 的部件,仍然是未知的。分别地,在这研究,从 Pieris melete 的 Hsp90 的互补 DNA, Pieris rapae 和 Pieris canidia,命名 PmHsp90, PrHsp90 和 PcHsp90 被克隆并且定序。推出的氨基酸顺序分别地与 82.6, 82.6 和 82.7 kDa 的一个通常认为的分子的团由 718 氨基酸残余组成了。氨基酸序列在 Hsp90 家庭和合拢活动区域的 bHLH 蛋白质五个保存签名主题包含了所有。响应夏天 diapause 和冬季 diapause 的 PmHsp90 的微分表示模式,与热 / 寒冷应力有关,被调查。冷应力在夏天和冬季 diapause 蛹,然而并非在 nondiapause 个人导致了 Hsp90 upregulation。热吃惊 upregulated PmHsp90 逐渐地随在夏天 diapause,和 PmHsp90 的温度的增加是很快在冬季 diapause 的 upregulated。在 39 点在 30 min 热以后震惊牥捡慥???????? ???? ???? 吗?? | Wu, Yue‐Kun Zou, Chao Fu, Dao‐Meng Zhang, Wan‐Na Xiao, Hai‐Jun | 2018 | Insect Science2018，25，2： | 5 |
| 10 | Preparation of Electrospun PVDF Nanofiber Composite Filter Medium and Its Application in Air Filtration显示文摘Nanofibrous media with both high particle interception efficiency and robust air permeability has broad technological applications in areas including individual protection, industrial security, and environmental governance. However, producing such filtration media has proven to be extremely challenging. Here we reported an approach to preparing and fabricating a polyvinylidene fluoride (PVDF) nanofiber composite filter medium composed of 2D PVDF nanofiber nets and a stable substrate via onestep electrospinning for effective air filtration. PVDF nanofibers are obtained by adjusting the electrospinning process. With the combined properties of ultrasmall diameter, high porosity, and a bonded scaffold, the resulting PVDF nanofiber composite filter medium exhibits a robust high filtration efficiency of 99.901%(equivalent to an F9 rating) for 0.4 mm particles and a long service life (a large dust holding capacity of 36 g/m^2) for ultrafine airborne particles based on the sieving principle and surface filtration behavior. The successful synthesis of PVDF nanofibers medium would not only make it a promising candidate for air filtration, but also provide new insights into the design and development of composite nanofiber structures for various applications. | Qi Du Na Wang Wen Liu XueFeng Chen Yue Xu Ying Wan | 2019 | Paper And Biomaterials2019，4，1： | 5 |
| 11 | Immunological Evaluation of a Novel Mycobacterium tuberculosis Antigen Rv0674显示文摘Objective This study aimed to characterize the diagnostic and vaccine potential of a novel Mycobacterium tuberculosisantigen Rv0674. Methods To evaluate thediagnostic potential and antigenicity of Rv0674, IgG was evaluated using ELISA and interferon (IFN)-γ was done by using ELISpot assay among TB patients and healthy donors. For immunogenicity evaluation, BALB/c mice were immunized with Rv0674. Cytokine production was determined by cytokine release assay using an ELISA kit, and the antibodies were tested using ELISA. Results The results of serum Elisa tests showed that Rv0674 specific immunoglobulin G (IgG) response was higher in TB patients than negative controls. And Rv0674 had good performance in serological test with sensitivity and specificity of 77.1% and 81.1%, respectively. While it shows poor sensitivity and specificity of 26.23% and 79.69% for IFN-γ tests. In BALB/c mice, Rv0674 adjuvant by DDA/PolyI:C could also induce a high level of IFN-γ, interleukin-2 and interleukin-6 as well as a high IgG titer in both high-and low-dose groups indicating that Rv0674 is essential in humoral and cellular immunity. Moreover, the cytokine profile and IgG isotypecharacterized Rv0674 as a Th1/Th2-mixed-type protective immunity with the predominance of Th1 cytokines. Conclusion Rv0674 may be a good potential candidate for the development of TB serological diagnosis and a new TB vaccine. | XIAO Tong Yang LIU Hai Can LI Xiao Qin HUANG Ming Xiang LI Gui Lian LI Na YAN Yu Han LUO Qiao WANG Xue Zhi LI Ma Chao WAN Kang Lin | 2019 | Biomedical and Environmental Sciences2019，32，6： | 3 |
| 12 | A New Compound from the Root of Salvia przewalskii Maxim显示文摘Neo-przewaquinone A was isolated from the root of Salvia przewalskii Maxim. The structure elucidation and 1H, 13C NMR assignments were achieved by spectroscopic method. | Wan Sheng CHEN*, Zhao Yang TAO, Wei Dong ZHANG, Lian Na SUN School of Pharmacy, Second Military Medical University, Shanghai 200433 | 2003 | Chinese Chemical Letters2003，14，7： | 3 |
| 13 | Effects of New Rural Cooperative Medical Scheme on Medical Service Utilization and Medical Expense Control of Inpatients: A 3-year Empirical Study of Hainan Province in China显示文摘 | Tao Dai Hong-Pu Hu Xu Na Ya-Zi Li Yan-Li Wan Li-Qin Xie | 2016 | Chinese Medical Journal2016，，11： | 3 |
| 14 | Comprehensive comparison on the chemical metabolites and taste evaluation of tea after roasting using untargeted and pseudotargeted metabolomics显示文摘Roasting is a common manufacture technology for processing various teas.It is not only used in decreasing the water content of finished tea,but also improving the flavor of teas.In the present study,the roasted and non-roasted teas were compared by liquid-chromatography mass spectrometry and sensory evaluation.The roasted tea tasted less bitter and astringent.The content of main galloylated and simple catechins,caffeine and theobromine in roasted were significantly lower than non-roasted teas.Targeted taste-compounds metabolomics revealed that(-)-epigallocatechin gallate,kaempferol-glucose-rhamnose-glucose and(-)-epicatechin gallate were main contributors tightly correlated to astringent intensity.Flavonol glycosides including kaempferol-glucose,quercetin-glucose,kaempferol-glucose-rhamnose-glucose,and quercetin-glucose-rhamnose-glucose in roasted teas were also significantly less than non-roasted teas.To study the chemical changes during roasting,tea with a strong astringency was roasted under 80,100,120,140,and 160°C.With the increase of roasting temperature,the bitter and astringent intensity of tea was gradually decreased,but the main astringent compounds including(-)-epigallocatechin,(-)-epigallocatechin gallate and kaempferol/quercetin glycosides were irregularly varied with temperature.The Pearson correlation coefficient analysis suggested procyanidin B2,coumaroylquinic acids and gallotannins were tightly correlated to the astringent and bitter perceptions,while N-ethyl-2-pyrrolidonesubstituted flavan-3-ols were negatively correlated. | Zongde Jiang Zisheng Han Mingchun Wen Chi-Tang Ho You Wu Yijun Wang Na Xu Zhongwen Xie Jinsong Zhang Liang Zhang Xiaochun Wan | 2022 | Food Science and Human Wellness2022，11，3： | 3 |
| 15 | Screening ofα-glucosidase inhibitors in large-leaf yellow tea by offline bioassay coupled with liquid chromatography tandem mass spectrometry显示文摘Larger-leaf yellow tea(LYT)is a characteristic type of Chinese tea produced in Huoshan County,Anhui Province,which is made by mature leaves with stems.According to recent report,LYT showed competitive effects in anti-hyperglycemia in comparison to other teas such as green or black tea.However,the bioactive compounds of LYT are still undiscovered so far.For this purpose,5 fractions of LYT were prepared by sequential extraction.The in vitro bioassay results indicated that the ethyl acetate fraction of LYT had the strongest inhibitory effects onα-glucosidase andα-amylase.Fluorescence-quenching analysis and proteinbinding test revealed that the compounds of ethyl acetate fraction could inhibitα-glucosidase andα-amylase activities through binding to enzymes or other mechanisms.All chromatographic peaks of high-performance liquid chromatography(HPLC)of ethyl acetate fraction were separated and collected.The purified compounds were identified by liquid chromatography-mass spectrometry(LC-MS),and subsequently screened by calculating their inhibition ratio onα-glucosidase at the real concentration in LYT infusion.The results showed that(-)-epigallocatechin gallate,(-)-gallocatechin gallate,caffeine,N-ethyl-2-pyrrolidone-substituted flavan-3-ols were effective inhibitors forα-glucosidase. | You Wu Zisheng Han Mingchun Wen Chi-Tang Ho Zongde Jiang Yijun Wang Na Xu Zhongwen Xie Jinsong Zhang Liang Zhang Xiaochun Wan | 2022 | Food Science and Human Wellness2022，11，3： | 3 |
| 16 | Effects of a combination of Japanese Raisin Tree Seed and Flower of Lobed Kudzuvine against acute alcohol-induced liver injury in mice显示文摘Objective:The Flower of Lobed Kudzuvine[Pueraria lobata(Willd.)Ohwi;Gehua in Chinese;GH]and Japanese Raisin Tree Seed(Hovenia dulcis Thunnb.;Zhijuzi in Chinese;ZJZ)are herbs that have been used in China for the treatment of alcohol intoxication and liver diseases.We aimed to evaluate the hepatoprotective potential of a combination of these in mice with acute alcohol-induced liver injury,and to elucidate the mechanisms involved.Methods:Male ICR mice were randomly allocated to six groups:a control group,an alcohol-administered group,and groups that were administered alcohol and one of silibinin,the GH,the ZJZ or a GH-ZJZ combination(at a ratio of 2:1).Animals were orally administered 56%alcohol(Er Guo-tou white spirit,0.12 mL/10 g/d)for 10 days and at the end of this period,hepatic biochemical indicators,antioxidant parameters,alcohol metabolic enzymes,and histopathologic changes were evaluated.Moreover,the expression of the signaling molecules KEAP1,NRF2,and AQP9 were measured by qRT-PCR and western blotting.Results:Compared with the model group,GH-ZJZ(2:1)had lower serum ALT(12.15±0.39,P紏.003),AST(104.07±1.03,P紏.001),and ALP(148.09±2.55,P紏.010)activities,and lower TC(1.97±0.05,P紏.001)and TG(1.54±0.07,P?.002)concentrations.GH-ZJZ(2:1)also significantly increased the hepatic activities of SOD and GSH(4.24±0.25 and 1.57±0.06,respectively;both P<.01),reduced the ROS and MDA concentrations(97.50±3.00 and 2.39±0.19,respectively;both P<.01),and upregulated Nrf2 expression(P<.01).GH-ZJZ(2:1)significantly reduced the expression of KEAP1 and AQP9 in the liver,compared with alcohol-administered mice(P<.01).Importantly,the GH-ZJZ combination caused a more marked improvement in acute liver injury than GH or ZJZ alone.Conclusion:We have demonstrated protective effects of GH-ZJZ(2:1)against acute alcohol-induced hepatic injury,and shown that these effects may be associated with improvements in lipid and alcohol metabolism,antioxidant capacity,and lipid peroxidation. | Wan Xu Shaohong Chen Gansheng Zhong Haiyan Liu Linlin Xiu Xue Yu Feng Chen Na Li Yanmin Lv | 2020 | Journal of Traditional Chinese Medical Sciences2020，7，1： | 2 |
| 17 | Induction of priming by cold stress via inducible volatile cues in neighboring tea plants^FA显示文摘Plants have evolved sophisticated defense mechanisms to overcome their sessile nature.However,if and how volatiles from cold‐stressed plants can trigger interplant communication is still unknown.Here,we provide the first evidence for interplant communication via inducible volatiles in cold stress.The volatiles,including nerolidol,geraniol,linalool,and methyl salicylate,emitted from cold‐stressed tea plants play key role(s)in priming cold tolerance of their neighbors via a C‐repeatbinding factors‐dependent pathway.The knowledge will help us to understand how plants respond to volatile cues in cold stress and agricultural ecosystems. | Mingyue Zhao Lu Wang Jingming Wang Jieyang Jin Na Zhang Lei Lei Ting Gao Tingting Jing Shangrui Zhang Yi Wu Bin Wu Yunqing Hu Xiaochun Wan Wilfried Schwab Chuankui Song | 2020 | Journal of Integrative Plant Biology2020，62，10： | 2 |
| 18 | Isocitrate lyase from Mycobacterium tuberculosis promotes survival of Mycobacterium smegmatis within macrophage by suppressing cell apoptosis显示文摘背景 Isocitrate lyase ( ICL )以前被表明 .Presently 在 Mycobacterium 肺结核(鱼雷快艇)的细胞内部的新陈代谢起一个枢轴的作用证据的 severallines 建议从鱼雷快艇( MTB-ICL )的 ICL 可以在在鱼雷快艇和主人 macrophage.However 之间的相互作用起一些作用,在 MTB-ICL 和主人 macrophage.Methods MTB-icl 和 M 之间的相互作用上没有研究。smegmatis (MS )-icl 基因被聚合酶链反应(PCR ) 放大并且克隆进 E。由 electroporation 获得 recombinant 梭 plasmids PMTB-icl 和 pMS-icl.Following 转变进 MS 的 coli-mycobacterium 梭 plasmid pUV15 , pMTB-icl 和 pMS-icl 的表示被 recombinant plasmids 的 -PCR.The 表示从 pUVl 导出的反向的 transcriptase ( RT )验证 5 rMS 什么时候是由巨噬细胞的 phagocytized ,也经由荧光 microscope.Ms 1-2c 被验证, rMS-pUV15 , rMS-pMS-icl 和 rMS-pMTB-icl 被用来感染 RAW264.7 房间,细胞内部的 MS 的 survivaI 是 | LI Jun-ming, LI Na, ZHU Dao-yin WAN La-gen HE Yong-lin YANG Chun | 2008 | Chinese Medical Journal2008，，12： | 2 |
| 19 | Ischemic or toxic injury: A challenging diagnosis and treatment of drug-induced stenosis of the sigmoid colon显示文摘A 48-year-old woman was admitted with 15-mo history of abdominal pain, diarrhea and hematochezia, and 5-mo history of defecation difficulty. She had been successively admitted to nine hospitals, with an initial diagnosis of inflammatory bowel disease with stenotic sigmoid colon. Findings from computed tomography virtual colonoscopy, radiography with meglumine diatrizoate, endoscopic balloon dilatation, metallic stent implantation and later overall colonoscopy, coupled with the newfound knowledge of compound Qingdai pill-taking, led to a subsequent diagnosis of ischemic or toxic bowel disease with sigmoid colon stenosis. The patient was successfully treated by laparoscopic sigmoid colectomy, and postoperative pathological examination revealed ischemic or toxic injury of the sigmoid colon, providing a final diagnosis of druginduced sigmoid colon stenosis. This case highlights that adequate awareness of drug-induced colon stenosis has a decisive role in avoiding misdiagnosis and mistreatment. The diagnostic and therapeutic experiences learnt from this case suggest that endoscopic balloon expansion and colonic metallic stent implantation as bridge treatments were demonstrated as crucial for the differential diagnosis of benign colonic stenosis. Skillful surgical technique and appropriate perioperative management helped to ensure the safety of our patient in subsequent surgery after long-term use of glucocorticoids. | Zong-Ming Zhang Xiang-Chun Lin Li Ma An-Qin Jin Fang-Cai Lin Zhuo Liu Li-Min Liu Chong Zhang Na Zhang Li-Juan Huo Xue-Liang Jiang Feng Kang Hong-Jun Qin Qiu-Yang Li Hong-Wei Yu Hai Deng Ming-Wen Zhu Zi-Xu Liu Bai-Jiang Wan Hai-Yan Yang Jia-Hong Liao Xu Luo You-Wei Li Wen-Ping Wei Meng-Meng Song Yue Zhao Xue-Ying Shi Zhao-Hui Lu | 2017 | World Journal of Gastroenterology2017，23，21： | 2 |
| 20 | Elastic chitosan conduits with multiple channels and well defined microstructure显示文摘 | Jixiang Zhu Yi Xiong Chenguang Zeng Na Qiang Daping Quan Jun Wan | 2012 | International Journal of Biological Macromolecules (-)2012，，1： | 1 |
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