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| 1 | Cytokines and hepatocytes显示文摘 | Geller DA Nussler AK Silvio MDI et al | 1993 | Proc Natl Acad Sci USA1993，90，12： | 1 |
| 2 | Assessment of tobacco heating system 2.4 on osteogenic differentiation of mesenchymal stem cells and primary human osteoblasts compared to conventional cigarettes显示文摘BACKGROUND Cigarette smoking(CS)is the most common method of consuming tobacco.Deleterious effects on bone integrity,increased incidence of fractures,and delayed fracture healing are all associated with CS.Over 150 of the 6500 molecular species contained in cigarette smoke and identified as toxic compounds are inhaled by CS and,via the bloodstream,reach the skeletal system.New technologies designed to develop a reduced-risk alternative for smokers are based on electronic nicotine delivery systems,such as e-cigarettes and tobacco heating systems(THS).THS are designed to heat tobacco instead of burning it,thereby reducing the levels of harmful toxic compounds released.AIM To examine the effects of THS on osteoprogenitor cell viability and function compared to conventional CS.METHODS Human immortalized mesenchymal stem cells(n=3)and primary human preosteoblasts isolated from cancellous bone samples from BG Unfall Klinik Tübingen(n=5)were osteogenically differentiated in vitro with aqueous extracts generated from either the THS 2.4“IQOS”or conventional“Marlboro”cigarettes for up to 21 d.Cell viability was analyzed using resazurin conversion assay(mitochondrial activity)and calcein-AM staining(esterase activity).Osteogenic differentiation and bone cell function were evaluated using alkaline phosphatase(AP)activity,while matrix formation was analyzed through alizarin red staining.Primary cilia structure was examined by acetylatedα-tubulin immunofluorescent staining.Free radical production was evaluated with 2’,7’-dichlorofluoresceindiacetate assay.RESULTS Our data clearly show that THS is significantly less toxic to bone cells than CS when analyzed by mitochondrial and esterase activity(P<0.001).No significant differences in cytotoxicity between the diverse flavors of THS were observed.Harmful effects from THS on bone cell function were observed only at very high,non-physiological concentrations.In contrast,extracts from conventional cigarettes significantly reduced the AP activity(by two-fold)and matrix mineralization(four-fold)at low concentrations.Additionally,morphologic analysis of primary cilia revealed no significant changes in the length of the organelle involved in osteogenesis of osteoprogenitor cells,nor in the number of ciliated cells following THS treatment.Assessment of free radical production demonstrated that THS induced significantly less oxidative stress than conventional CS in osteoprogenitor cells.CONCLUSIONTHS was significantly less harmful to osteoprogenitor cells during osteogenesisthan conventional CS. Additional studies are required to confirm whether THS isa better alternative for smokers to improve delays in bone healing followingfracture. | Romina H Aspera-Werz Sabrina Ehnert Monja Müller Sheng Zhu Tao Chen Weidong Weng Johann Jacoby Andreas K Nussler | 2020 | World Journal of Stem Cells2020，12，8： | 1 |
| 3 | The combined treatment with L-arginine and methylprednisolone improves graft morphology and mucosal barrier function 显示文摘 | Wamecke HB Schirmeier A Nussler AK | 2002 | Transplant Proc2002，34，3： | 1 |
| 4 | Association of bcl-2,bax,bcl-XL and inter leukin-1 β-conveoting enzyme expression with initial response to chemotherapy in acute myeloid leukemia显示文摘 | Stoezer O J Nussler V Darsow M | 1996 | Leukemia1996，，10： | 1 |
| 5 | Diagnosis and treatment of arterial steal syndromes in liver transplant recipients 显示文摘 | Nussler NC Settmacher U Haase R | 2003 | Liver Transplant2003，9，： | 1 |
| 6 | Stimulation of the nitric oxide synthase pathway in human hepatocytes by cytokines and endotoxin显示文摘 | Nussler AK Di Silvio M Billiar TR Hoffman RA Geller DA Selby R | 1992 | J Exp Med1992，176，1： | 1 |
| 7 | Proinflammatory cytokines and endotoxin stimulate ICAM-1 gene expression and secretion by normal human hepatocytes 显示文摘 | Saloh S Nussler AK Liu ZZ | 1994 | Immunology1994，82，： | 1 |
| 8 | Present status and perspectives of cell-based therapies for liver diseases显示文摘 | Andreas Nussler Sarah Konig Michael Ott Etienne Sokal Bruno Christ Wolfgang Thasler Marc Brulport Geredn Gabelein Wiebke Schormann Maren Schulze Ewa Ellis Matthias Kraemer Frank Nocken Wolfgang Fleig Michael Manns Steven C. Strom Jan G. Hengstler | 2006 | Journal of Hepatology2006，，1： | 1 |
| 9 | Inflammation immunoregulation and inducible nitric oxide synthase显示文摘 | Andreas K Nussler Timothy A | 1993 | Journal of Leukocyte Biology1993，54，： | 1 |
| 10 | Diagnosis and treatment of arterial steal syndromes in liver transplant recipients 显示文摘 | Nussler NC Settmacher U Haase R | 2003 | Liver Transpl2003，9，6： | 1 |
| 11 | Indication,technique,and outcome of portal vein arterialization in orthotopic liver transplantation显示文摘 | Stange B Glanemann M Nussler NC | 2001 | Transplant Proc2001，33，12： | 1 |
| 12 | Circulating intercellular abhesion molecule-1(CICAM-1) in autiommune liver diseases and evidence for the production of ICAM-1,by Cytokinestimulater hepatoaytes显示文摘 | Thomson AW Satohs Nussler AK | 1994 | Clin EXP immunol1994，95，1： | 1 |
| 13 | Early posttraumatic increase in production of nitric oxide in humans显示文摘 | Nussler AK Rosch M | 1998 | Shock1998，10，4： | 1 |
| 14 | Venous complications after orthotopic liver transplantation 显示文摘 | Settmacer U Nussler NC Neuhaus P | 2000 | Clin Transplant2000，14，： | 1 |
| 15 | Evolving experience with prevention and treatment of splenic artery syndrome after orthotopic liver transplantation显示文摘 | Mogl MT Nussler NC Presser SJ | 2010 | Transplant Int2010，23，8： | 1 |
| 16 | Diagnosis and Treatment of Arterial Steal Syndromes in Liver Transplant Recipients显示文摘 | Nussler NC Settmacher U Haase R | 2003 | Liver Transpl2003，9，6： | 1 |
| 17 | Differentiation of in vitro-modified human peripheral blood monocyte into hepatocyte- like and pancreatic islet-like cells 显示文摘 | Ruhnker M Ungefroren H Nussler A | 2005 | Gastroenterology2005，128，7： | 1 |
| 18 | Inhibition of nitric oxide synthase does not influence urinary nitrite plus nitrate excretion after renal ischemic injury显示文摘 | Ogawa T Nussler AK Tuaumer E | 2002 | Langenbecks Arch Surg2002，386，7： | 1 |
| 19 | Protective role of HO-1 for alcohol-dependent liver damage显示文摘 | Nussler AK Hao L Knobeloch D Yao P | | 0，，： | 1 |
| 20 | In vivo induction of the nitric oxide pathway in hepatocytes after injection with irradiated malaria sporozoites, malaria blood parasites or adjuvants显示文摘 | Nussler AK Renia L Pasquetto V | 1993 | Eur J Immunol1993，23，4： | 1 |
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