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| 1 | Haplotype of prostaglandin synthase 2/cyclooxygenase 2 is involved in the susceptibility to inflammatory bowel disease显示文摘AIM: Prostaglandin G/H synthase 2 (PTGS2 or COX2) is one of the key factors in the cellular response to inflammation.PTGS2 is expressed in the affected intestinal segments of patients with inflammatory bowel diseases (IBD). In IBD patients, non-steroidal anti-inflammatory drugs, which have been shown to reduce both the production and activity of PTGS2, may activate IBD and aggravate the symptoms.We aimed at examining genetic variants of PTGS2 that may be risk factors for IBD.METHODS: We genotyped 291 individuals diagnosed with IBD and 367 controls from the Dutch population for the five most frequent polymorphisms of the PTGS2 gene.Clinical data were collected on all patients. DNA was extracted via normal laboratory methods. Genotyping was carried out using multiplex PCR followed by the Invader Assay and the 5' exonuclease assay (TaqMan). New polymorphism screening was performed by pre-screening with denaturing high-performance liquid chromatography,followed by fluorescent sequencing.RESULTS: Allele 5209G was weakly associated with Crohn's disease (odds ratio [OR] 1.63, 95% confidence interval [CI] 1.03-2.57), and allele 8473T with ulcerative colitis (OR 1.50, 95%CI 1.00-2.27). The haplotype including both alleles showed a strong association with IBD (OR 13.15, 95%CI 3.17-116.15). This haplotype, while rare (-0.3%) in the general population, is found more frequently in patients (3.5%).CONCLUSION: Our data suggest that this haplotype of PTGS2 contributes to the susceptibility of IBD. | David G Cox J Bart A Crusius Petra HM Peeters H Bas Bueno-de-Mesquita A Salvador Pe(n|~)a Federico Canzian | 2005 | World Journal of Gastroenterology2005，11，38： | 10 |
| 2 | Alleviated mucosal and neuronal damage in a rat model of Crohn's disease显示文摘AIM:To establish a rat model suitable to investigate the repetitive relapsing inflammations(RRI)characteristic to Crohn’s disease.METHODS:Colitis was induced by 2,4,6-trinitrobenzenesulfonic acid(TNBS).RRI were mimicked by repeating administrations of TNBS.Tissue samples were taken from control,once,twice and three times treated rats from the inflamed and adjacent non-inflamed colonic segments at different timepoints during the acute intestinal inflammation.The means of the ulcerated area were measured to evaluate the macroscopic mu-cosal damage.The density of myenteric neurons was determined on whole mounts by Hu C/Hu D immunohistochemistry.Heme oxygenase-1(HO-1)expression was evaluated by molecular biological techniques.RESULTS:TNBS-treated rats displayed severe colitis,but the mortality was negligible,and an increase of body weight was characteristic throughout the experimental period.The widespread loss of myenteric neurons,and marked but transient HO-1 up-regulation were demonstrated after the first TNBS administration.After repeated doses the length of the recovery time and extent of the ulcerous colonic segments were markedly decreased,and the neuronal loss was on a smaller scale and was limited to the inflamed area.HO-1 m RNA level was notably greater than after a single dose and overexpression was sustained throughout the timepoints examined.Nevertheless,the HO-1protein up-regulation after the second TNBS treatment proved to be transient.Following the third treatment HO-1 protein expression could not be detected.CONCLUSION:Experimentally provoked RRI may exert a protective preconditioning effect against the mucosal and neuronal damage.The persistent up-regulation of HO-1 m RNA expression may correlate with this. | Petra Talapka Lajos István Nagy Alexandra Pál Marietta Zita Poles Anikó Berkó Mária Bagyánszki László Géza Puskás éva Fekete Nikolett Bódi | 2014 | World Journal of Gastroenterology2014，20，44： | 3 |
| 3 | Structural and molecular features of intestinal strictures in rats with Crohn's-like disease显示文摘AIM: To develop a new rat model we wanted to gain a better understanding of stricture formation in Crohn's disease(CD).METHODS: Chronic colitis was induced locally by the administration of 2,4,6-trinitrobenzenesulfonic acid(TNBS). The relapsing inflammation characteristic to CD was mimicked by repeated TNBS treatments. Animals were randomly divided into control, once, twice and three times TNBS-treated groups. Control animals received an enema of saline. Tissue samples were taken from the strictured colonic segments and also adjacent proximally and distally to its 60, 90 or 120 d after the last TNBS or saline administrations. The frequency and macroscopic extent of the strictures were measured on digital photographs. The structural features of strictured gut wall were studied by light- and electron microscopy. Inflammation related alterations in TGF-beta 2 and 3, matrix metalloproteinases 9(MMP9) and TIMP1 m RNA and protein expression were determined by quantitative real-time PCR and western blot analysis. The quantitative distribution of caspase 9 was determined by post-embedding immunohistochemistry.RESULTS: Intestinal strictures first appeared 60 d after TNBS treatments and the frequency of them increased up to day 120. From day 90 an intact lamina epithelialis, reversible thickening of lamina muscularis mucosae and irreversible thickening of the muscularis externa were demonstrated in the strictured colonic segments. Nevertheless the morphological signs of apoptosis were frequently seen and excess extracellular matrix deposition was recorded between smooth muscle cells(SMCs). Enhanced caspase 9 expression on day 90 in the SMCs and on day 120 also in myenteric neurons indicated the induction of apoptosis. The m RNA expression profile of TGF-betas after repeated TNBS doses was characteristic to CD, TGF-beta 2, but not TGF-beta 3 was up-regulated. Overexpression of MMP9 and down-regulation of TIMP1 were demonstrated. The progressive increase in the amount of MMP9 protein in the strictures was also obvious between days 90 and 120 but TIMP1 protein was practically undetectable at this time.CONCLUSION: These findings indicate that aligned structural and molecular changes in the gut wall rather than neuronal cell death play the primary role in stricture formation. | Petra Talapka Anikó Berkó Lajos István Nagy Lalitha Chandrakumar Mária Bagyánszki László Géza Puskás éva Fekete Nikolett Bódi | 2016 | World Journal of Gastroenterology2016，22，22： | 3 |
| 4 | A Novel Line Immunoassay Based on Recombinant Virulence Factors Enables Highly Specific and Sensitive Serologic Diagnosis of Helicobacter pylori Infection显示文摘 | Luca Formichella Laura Romberg Christian Bolz Michael Vieth Michael Geppert Gereon G?ttner Christina N?lting Dirk Walter Wolfgang Schepp Arne Schneider Kurt Ulm Petra Wolf Dirk H. Busch Erwin Soutschek Markus Gerhard | 2013 | Clinical and Vaccine Immunology2013，，11： | 2 |
| 5 | Epitope mapping of cytochrome P4502D6 autoantigen in patients with chronic hepatitis C during α-interferon treatment显示文摘 | Georgios N Dalekos Heiner Wedemeyer Petra Obermayer-Straub Anne Kayser Ayse Barut Hannah Frank Michael P Manns | 1999 | Journal of Hepatology1999，，3： | 1 |
| 6 | Theoretical analysis of a quartz-enhanced photoacoustic spectroscopy sensor显示文摘 | PETRA N ZWECK J KOSTEREV A A | 2009 | Applied Physics B2009，94，4： | 1 |
| 7 | Digital synthesizer/mixer with hybrid CORDIC-multiplier architecture:error analysis and optimization显示文摘 | De CARO D PETRA N STROLLO A G M | 2009 | IEEE Transactions on circuit and systems-I: regular papers2009，56，2： | 1 |
| 8 | A 630 MHz, 76 mW direct digital frequency synthesizer using enhanced ROM compression technique 显示文摘 | STROLLO A G M CARO D D PETRA N | 2007 | IEEE J Sol Sta Cire2007，42，2： | 1 |
| 9 | Electroencephalography in the elderly显示文摘 | Davidson Petra N Davidson Keith A | 2012 | Neurodiagnostic Journal2012，52，1： | 1 |
| 10 | Practical Application of Digital Fractional-Order Controller to Temperature Control显示文摘 | PETRAS I VINAGRE B N | 2002 | Acta Montanistica Slovaca2002，7，2： | 1 |
| 11 | Accumulation and metabolism of the anticancer flavonoid 5,7- dimethoxyflavone compared to its unmethylated analog chrysin in the Atlantic killifish显示文摘 | Petra A Tsuji Richard N Winn Thomas Walle | 2006 | Chemico-Biological Interactions2006，164，： | 1 |
| 12 | Accumulation and metabolism of the anticancer flavonoid 5, -dimdthoxyflavone compared to its unmethylated analog chrysin in the Atlantic killifish显示文摘 | Petra A T Richard N W Thomas W | 2006 | Chem Biol Interact2006，164，4： | 1 |
| 13 | Intramyocardial Injection of Autologous Bone Marrow-Derived Ex Vivo Expanded Mesenchymal Stem Cells in Acute Myocardial Infarction Patients is Feasible and Safe up to 5 Years of Follow-up显示文摘 | Sander F. Rodrigo Jan Ramshorst Georgette E. Hoogslag Helèn Boden Matthijs A. Velders Suzanne C. Cannegieter Helene Roelofs Imad Younis Petra Dibbets-Schneider Willem E. Fibbe Jaap Jan Zwaginga Jeroen J. Bax Martin J. Schalij Saskia L. Beeres Douwe E. Ats | 2013 | Journal of Cardiovascular Translational Research2013，，5： | 1 |
| 14 | Characterization of mtDNA SNP typing and mixture ratio assessment with simultaneous real-time PCR quantification of both allelic states 显示文摘 | Harald N Michael D C Petra G | 2006 | Int J Leg Med2006，120，： | 1 |
| 15 | Hyperphosphorylation of the hepatitis C virus NS5A protein requires an active NS3 protease, NS4A, NS4B, and NS5A encoded on the same polyprotein显示文摘 | Petra N Angelica C Raffaele DF | 1999 | J virol1999，73，12： | 1 |
| 16 | Development and validation of a second-generation metal-on-metal bearing显示文摘 | Claude B. Rieker Rolf Sch?n Petra K?ttig | 2004 | The Journal of Arthroplasty2004，，8： | 1 |
| 17 | More Frequent Flooding Changes in Flood Frequency in Switzerland sinee 1850 显示文摘 | Petra S F Felix N | 2010 | Journal of Hydrology2010，381，： | 1 |
| 18 | Corporate governance and chief executive officer compensation 显示文摘 | Petra S T Dorata N T | 2008 | Corporate Govern- ance2008，8，2： | 1 |
| 19 | Cytochrome P450 2A6: a new hepatic autoantigen in patients with chronic hepatitis C virus infection显示文摘 | Georgios N Dalekos Petra Obermayer-Straub Moritz Bartels Takashi Maeda Anne Kayser Sabine Braun Stephanie Loges Eleonore Schmidt M.Eric Gershwin Michael P Manns | 2003 | Journal of Hepatology2003，，5： | 1 |
| 20 | Theoretical Analysis of aQuartz-Enhanced Photoacoustic Spectroscopy Sensor显示文摘 | Petra N Zweck J Kosterev A A | 2009 | AppliedPhysics B:Lasers and Optics2009，94，4： | 1 |
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