|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Enhancement of human ACAT1 gene expression to promote the macrophage-derived foam cell formation by dexamethasone显示文摘In macrophages, the accumulation of cholesteryl esters synthesized by the activated acyl-coenzyme A:cholesterol acyltransferase-1 (ACAT1) results in the foam cell formation, a hallmark of early atherosclerotic lesions. In this study,with the treatment of a glucocorticoid hormone dexamethasone (Dex), lipid staining results clearly showed the large accumulation of lipid droplets containing cholesteryl esters in THP- 1-derived macrophages exposed to lower concentration of the oxidized low-density lipoprotein (ox-LDL). More notably, when treated together with specific anti-ACAT inhibitors, the abundant cholesteryl ester accumulation was markedly diminished in THP-l-derived macrophages, confirming that ACAT is the key enzyme responsible for intracellular cholesteryl ester synthesis. RT-PCR and Western blot results indicated that Dex caused up-regulation of human ACAT1 expression at both the mRNA and protein levels in THP-1 and THP- 1-derived macrophages. The luciferase activity assay demonstrated that Dex could enhance the activity of human ACAT1 gene P1 promoter, a major factor leading to the ACAT1 activation, in a cell-specific manner.Further experimental evidences showed that a glucocorticoid response element (GRE) located within human ACAT1gene P1 promoter to response to the elevation of human ACAT1 gene expression by Dex could be functionally bound with glucocorticoid receptor (GR) proteins. These data supported the hypothesis that the clinical treatment with Dex,which increased the incidence of atherosclerosis, may in part due to enhancing the ACAT1 expression to promote the accumulation of cholesteryl esters during the macrophage-derived foam cell formation, an early stage of atherosclerosis. | LiYANG JinBoYANG JiaCHEN GuangYaoYU PeiZHOU LeiLEI ZhenZhenWANG CatherineCYCHANG XinYingYANG TaYuanCHANG BoLiangLI | 2004 | Cell Research2004，14，4： | 24 |
| 2 | Role of autophagy in the pathogenesis of multiple sclerosis显示文摘Autophagy plays an important role in maintaining the cellular homeostasis. One of its functions is to degrade unnecessary organelles and proteins for energy recycling or amino-acids for cell survival. Ablation of autophagy leads to neurodegeneration. Multiple sclerosis(MS), a permanent neurological impairment typical of chronic inflammatory demyelinating disorder, is an auto-immune disease of the central nervous system(CNS). Autophagy is tightly linked to the innate and adaptive immune systems during the autoimmune process, and several studies have shown that autophagy directly participates in the progress of MS or experimental autoimmune encephalomyelitis(EAE, a mouse model of MS). Dysfunction of mitochondria that intensively influences the autophagy pathway is one of the important factors in the pathogenesis of MS. Autophagy-related gene(ATG) 5 and immune-related GTPase M(IRGM) 1 are increased, while ATG16L2 is decreased, in T-cells in EAE and active relapsing-remitting MS brains. Administration of rapamycin, an inhibitor of mammalian target of rapamycin( m TOR), ameliorates relapsing-remitting EAE. Inflammation and oxidative stress are increased in MS lesions and EAE, but Lamp2 and the LC3-II/LC3-I ratio are decreased. Furthermore, autophagy in various glial cells plays important roles in regulating neuro-inflammation in the CNS, implying potential roles in MS. In this review, we discuss the role of autophagy in the peripheral immune system and the CNS in neuroinflammation associated with the pathogenesis of MS. | Peizhou Liang Weidong Le | 2015 | Neuroscience Bulletin2015，31，4： | 19 |
| 3 | Patterns of DNA cytosine methylation between haploids and corresponding diploids in rice显示文摘Eighteen pairs of diploid-haploid twin-seedlings were identified and screened out fromspecial rice SARII-628 population. Five pairs of themwere selected and randomly designated as A, B, C, Dand E. Simple sequence repeats (SSR) analysisshowed that there was no difference among 310 siteswhich indicated that there was no base mutation onDNA primary structure. DNA methylation plays animportant role in gene expression regulation duringgrowth and development stages in eukaryotes. Amodified AFLP technique (methylation-sensitiveAFLP, MSAP) was employed to detect the DNA me-thylation patterns in the 5′-CCGG sites of the fivepairs of twin-seedlings. Although no methylationmutation was detected among the five diploids,forty-three methylation mutation sites were foundfrom the corresponding haploids. The MSAP ratios,which were the ratios of MSAP sites to the total am-plified sites, in five haploids were 18.79%, 19.35%,18.49%, 18.45% and 18.75%, respectively. And cor-responding full methylation levels (5′-CmCGG indouble strands) of those haploids were 10.58%,11.3%, 10.11%, 10.09% and 10.34%, respectively.Both MSAP and full methylation levels in the fivehaploids were higher than that of their correspondingdiploids, which suggested that hypermethylation oc-curred in some 5′-CCGG sites. Five types of MASPpatterns among the five pairs of twin-seedlings weredetected as follows: (1) no changes, methylation lev-els were the same in both haploids and diploids; (2) demethylation, diploid was methylated but no me-thylation in the same site in haploid; (3) hypermethy-lation, the methylation level in haploid was higher than those in diploid; (4) hypomethylation, methyla-tion in haploid was lower than those in diploid; (5) undecided pattern, change trend of methylation lev-els in haploids was not decided. The bands of 18 sites were reclaimed, then sequenced and searched on website to determine the sites of those sequences on rice chromosomes. The result showed that the methylation mutation involved the whole rice genome and 12 pairs of chromosomes. The mutation was site-related and there were different mutation sites for different haploids. Compared to diploids, the higher methylation level in haploids might be a readjusting reaction to the decrease in ploidy for the sake of sur-vival. | ZHANG Hongyu PENG Hai LI Yun XU Peizhou WANG Xudong WU Xianjun | 2006 | Chinese Science Bulletin2006，51，14： | 15 |
| 4 | 5-Ethynyl-2′-deoxyuridine as a molecular probe of cell proliferation for high-content siRNA screening assay by “click” chemistry显示文摘Labelling and identification of proliferating cells is important for the study of physiological or pathological processes in high-content screening (HCS) assays. Here we describe ethynyl deoxyuridine (EdU) as a biomarker for the assessment of cell proliferation and clearly demonstrate the feasibility of the EdU-labelling method for use in HCS assays. EdU detection is highly robust, reproducible, technically simple, and well suited for automated segmentation, which provides an excellent al- ternative for setting up multiplexed HCS assays of siRNA, miRNA and small-molecule libraries. | CHEN MiaoJuan QU DeZhong CHI WeiLin WANG Wei REN XiaoShuai CONG ShuJie LIANG PeiZhou FENG ShiPeng ZHANG BiLiang | 2011 | Science China Chemistry2011，54，11： | 6 |
| 5 | Synthesis of Microporous Nitrogen-Rich Covalent-Organic Framework and Its Application in CO_(2) Capture显示文摘An imine-based nitrogen-rich covalent-organic framework(COF)was successfully synthesized using two tri-angular building units under solvothermal reaction condition.The gas adsorption properties of the obtained micro-porous nitrogen-rich COF were investigated.The results indicated that the activated COF material presented good up take capabilities of CO_(2) and CH_(4) at 61.2 and 43.4 cm^(3)·g^(−1)at 1 atm and 273 K,respectively,showing its applica-tion potential in selective gas capture and separation. | Qiang Gao Linyi Bai Xiaojing Zhang Peng Wang Peizhou Li Yongfei Zeng Ruqiang Zou Yanli Zhao | 2015 | Chinese Journal of Chemistry2015，33，1： | 2 |
| 6 | Expression of multi-functional cellulase gene mfc in Coprinus cinereus under control of different basidiomycete promoters显示文摘 | CHENG Shujie YANG Peizhou GUO Liqiong | 2009 | Bioresource Technology2009，100，19： | 1 |
| 7 | Recombinantmuhi-functional cellulase activity in submerged fer- mentation of lignocellulosicwaster显示文摘 | Yang Peizhou Guo Liqiong Cheng Shujie | 2011 | Renewable Energy2011，，36： | 1 |
| 8 | ZIF-8 immobilized nickel nanoparticles: highly effective catalysts for hydro- gen generation from hydrolysis of ammonia borane 显示文摘 | Li Peizhou Aranishi K Xu Qiang | 2012 | Chem Commun2012，48，26： | 1 |
| 9 | , Expression of multi-function cellulose gene mfc in Coprinus cinereus under control of different basidiomycete promoters 显示文摘 | Shujie Cheng Peizhou Yang Liqiong Guo | 2009 | Bioresource Technology2009，100，： | 1 |
| 10 | A rationally designed nitrogen-rich metal-organic framework and its ex- ceptionallyhigh CO2 and H2 uptake capability 显示文摘 | Wang Xiaojun Li Peizhou Chen Yifei | 2013 | Sci Rep2013，3，： | 1 |
| 11 | Dynamic Programmable Scheduling Mechanism of GEPON 显示文摘 | Aihan YIN Peizhou ZHANG Wei ZHOU | 2009 | Journal of Light wave Technology2009，，27： | 1 |
| 12 | Expression of multi-functional cellulase gene mfc in Coprinus cinereus under control of different basidiomycete promoters显示文摘 | Shujie Cheng Peizhou Yang Liqiong Guo | 2009 | Bioresource Technology2009，100，44： | 1 |
| 13 | Hsp70 gene expression in four hatchery Pacific abalone,Haliotis discus hannai Ino,populations used for marker-assisted selection显示文摘 | Cheng Peizhou Liu Xiao Zhang Guofan | 2006 | Aquaculture Research2006，37，： | 1 |
| 14 | Cloning and expression analysis of an inducible HSP70 gene from Pacific abalone,Haliotis discus hannai Ino显示文摘 | Cheng Peizhou Liu Xiao Zhang Guofan | 2006 | Fish and Shellfish Immunology2006，，： | 1 |
| 15 | Hsp70 gene expression in four hatchery Pacific abalone,Haliotis discus hannai Ino,populations used for marker-assisted selection显示文摘 | Cheng Peizhou Liu Xiao Zhang Guofan | 2006 | Aquaculture Research2006，37，： | 1 |
| 16 | KPNB1 , XPO7 and IPO8 Mediate the Translocation of NF‐κB /p65 into the Nucleus显示文摘 | Peizhou Liang Haiyan Zhang Guoxin Wang Suping Li Shujie Cong Yingyun Luo Biliang Zhang | 2013 | Traffic2013，，11： | 1 |
| 17 | Ligand Engineering in Tin-Based Perovskite Solar Cells显示文摘Perovskite solar cells(PSCs)have attracted aggressive attention in the photovoltaic field in light of the rapid increasing power conversion efficiency.However,their large-scale application and commercialization are limited by the toxicity issue of lead(Pb).Among all the lead-free perovskites,tin(Sn)-based perovskites have shown potential due to their low toxicity,ideal bandgap structure,high carrier mobility,and long hot carrier lifetime.Great progress of Sn-based PSCs has been realized in recent years,and the certified efficiency has now reached over 14%.Nevertheless,this record still falls far behind the theoretical calculations.This is likely due to the uncontrolled nucleation states and pronounced Sn(Ⅳ)vacancies.With insights into the methodologies resolving both issues,ligand engineering-assisted perovskite film fabrication dictates the state-of-the-art Sn-based PSCs.Herein,we summarize the role of ligand engineering during each state of film fabrication,ranging from the starting precursors to the ending fabricated bulks.The incorporation of ligands to suppress Sn~(2+)oxidation,passivate bulk defects,optimize crystal orientation,and improve stability is discussed,respectively.Finally,the remained challenges and perspectives toward advancing the performance of Sn-based PSCs are presented.We expect this review can draw a clear roadmap to facilitate Sn-based PSCs via ligand engineering. | Peizhou Li Xiangrong Cao Jingrui Li Bo Jiao Xun Hou Feng Hao Zhijun Ning Zuqiang Bian Jun Xi Liming Ding Zhaoxin Wu Hua Dong | 2023 | Nano-Micro Letters2023，15，10： | 0 |
| 18 | Driver Fatigue Detection System Based on Colored and Infrared Eye Features Fusion显示文摘Real-time detection of driver fatigue status is of great significance for road traffic safety.In this paper,a proposed novel driver fatigue detection method is able to detect the driver’s fatigue status around the clock.The driver’s face images were captured by a camera with a colored lens and an infrared lens mounted above the dashboard.The landmarks of the driver’s face were labeled and the eye-area was segmented.By calculating the aspect ratios of the eyes,the duration of eye closure,frequency of blinks and PERCLOS of both colored and infrared,fatigue can be detected.Based on the change of light intensity detected by a photosensitive device,the weight matrix of the colored features and the infrared features was adjusted adaptively to reduce the impact of lighting on fatigue detection.Video samples of the driver’s face were recorded in the test vehicle.After training the classification model,the results showed that our method has high accuracy on driver fatigue detection in both daytime and nighttime. | Yuyang Sun Peizhou Yan Zhengzheng Li Jiancheng Zou Don Hong | 2020 | Computers, Materials & Continua2020，，6： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员