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| 1 | Biomass carbon stocks in China's forests between 2000 and 2050:A prediction based on forest biomass-age relationships显示文摘China's forests are characterized by young forest age,low carbon density and a large area of planted forests,and thus have high potential to act as carbon sinks in the future.Using China's national forest inventory data during 1994-1998 and 1999-2003,and direct field measurements,we investigated the relationships between forest biomass density and forest age for 36 major forest types.Statistical approaches and the predicted future forest area from the national forestry development plan were applied to estimate the potential of forest biomass carbon storage in China during 2000-2050.Under an assumption of continuous natural forest growth,China's existing forest biomass carbon(C) stock would increase from 5.86 Pg C(1 Pg=1015 g) in 1999-2003 to 10.23 Pg C in 2050,resulting in a total increase of 4.37 Pg C.Newly planted forests through afforestation and reforestation will sequestrate an additional 2.86 Pg C in biomass.Overall,China's forests will potentially act as a carbon sink for 7.23 Pg C during the period 2000-2050,with an average carbon sink of 0.14 Pg C yr-1.This suggests that China's forests will be a significant carbon sink in the next 50 years. | XU Bing,GUO ZhaoDi,PIAO ShiLong & FANG JingYun* Department of Ecology,College of Urban and Environmental Sciences,and Key Laboratory for Earth Surface Processes of the Ministry of Education,Peking University,Beijing 100871,China | 2010 | Science China(Life Sciences)2010，53，7： | 61 |
| 2 | Clinical short-term results of radiofrequency ablation in liver cancers显示文摘AIM: To study local therapeutic efficacy, side effects, andcomplications of radiofrequency ablation (RFA), whichis emerging as a new method for the treatment ofpatients with hepatocellular carcinoma (HCC) withcirrhosis or chronic hepatitis and metastatic liver cancer.METHODS: Thirty-six patients with primary andsecondary liver cancers (21 with primaryhepatocellular carcinoma, 12 with colorectal cancerliver metastases and 3 with other malignant livermetastases), which were considered not suitable forcurative resection, were include in this study. Theywere treated either with RFA (RITA2000, MountainView, California, USA) percutaneously (n=20)orintraoperatively (n=16).The procedures wereperformed using the ultrasound guidance. The qualityof RFA were based on monitoring of equipments andsubject feeling of the practitioners. Patients treatedwith RFA was followed according to clinical findings,radiographic images, and tumor markers.RESULTS: Thirty-six patients underwent RFA for 48nodules. RFA was used to treat an average 1.3 lesionsper patient, and the median size of treated lesions was2.5 cm (range, 0.5-9 cm). The average hospital staywas 5.6 days overall (2.8 days for percutaneous casesand 7.9 days for open operations). Seven patientsunderwent a second RFA procedure (sequentialablations). Sixteen HCC patients with a high level ofalpha fetoprotein (AFP) and 9 colorectal cancer livermetastases patients with a high level of serumcarcinoembryonic antigen (CEA) have a great reductionbenefited from RFA. Four (11.1 %) patients hadcomplications: one skin burn; one postoperativehemorrhage; one cholecystitis and one hepatic abscessassociated with percutaneous ablations of a largelesion. There were 4 deaths: 3 patients died from localand system diseases (1 at 7 month, 1 at 9 month, and 1at 12 month), 1 patients died from cardiovascular shock,but no RFA-related death. At a median follow-up of 10months (range, 1-24 months), 6 patients (16.7 %) hadrecurrences at an RFA site, and 20 patients (56.7 %)remained clinically free of disease.CONCLUSION: RF ablation appears to be an effective,safe, and relatively simple procedure for the treatmentof unresectable liver cancers. The rate and severity ofcomplications appear acceptable. However, furtherstudy is necessary to assess combination with othertherapies, long-term recurrence rates and effect onoverall survival. | Hong-Chi Jiang Lian-Xin Liu Da-Xun Piao Jun Xu An-Long Zhu Wei-Hui Zhang Lin-Feng Wu Department of Surgery,the First Clinical College,Harbin Medical University,Harbin 150001,Heilongjiang Province,China Min Zheng Department of Ultrasound Diagnosis,the First Clinical College,Harbin Medical University,Harbin 150001,Heilongjiang Province,China Shu-Yi Qi Second Inpatient Department,the First Clinical College,Harbin Medical University,Harbin 150001,Heilongjiang Province,China | 2002 | World Journal of Gastroenterology2002，8，4： | 33 |
| 3 | Morphological and serum hyaluronic acid, laminin and type Ⅳ collagen changes in dimethylnitrosamine-induced hepatic fibrosis of rats显示文摘瞄准:学习词法并且浆液透明质酸(哈) , laminin (行) ,和类型 IV 骨胶原在 dimethylnitrosamine (DMN ) 导致的老鼠的肝的纤维变性变化。方法:肝纤维变性的老鼠模特儿被 DMN 劝诱。浆液哈,类型 IV 骨胶原,和行被 ELISA 测量。肝 / 重量索引和词法变化在 d 上在电子显微镜下面被检验 7, 14, 21,和 28 由免疫组织化学的高山哈象天狼星红一样染色的平滑肌肌动朊 alpha-SMA 并且他染色。结果:浆液的层次哈,类型 IV 骨胶原和行显著地从 d 增加了 7 到 d 28 (P = 0.043 ) 。肝 / 重量索引在 d 上增加了 7 并且在 d 上减少了 28。在模型组,老鼠肝染色了与他和天狼星红在中央静脉显示出明显的出血和坏死肝 d 上的 10 腹片 7。薄纤维变性氏族一被形成在 d 上加入肝的中央区域 14。alpha-SMA 的数字积极房间显著地在模型组被增加。过渡肝的星形细胞在电子显微镜下面被观察。在模型组的所有老鼠在肝的实质和 alpha-SMA 的一个网络显示出微榴状的纤维变性积极房间。典型 myofibroblasts 在含纤维的中隔的核心被嵌入。比作控制组,骨胶原纤维变性的区域密度百分比并且病理学的分级在不同 d (7, 14,和 28 ) 上在模型组(P<0.05 ) 是显著地不同的。在肝的织物的骨胶原纤维变性的区域密度百分比与浆液的层次有积极关联哈,行,和类型 IV 骨胶原。结论:词法并且浆液哈,类型 IV 骨胶原,和行在老鼠在导致 DMN 的肝纤维变性被改变。 | Chun-Hui Li Dong-Ming Piao Wen-Xie Xu Zheng-Ri Yin Jing-Shun Jin Zhe-Shi Shen | 2005 | World Journal of Gastroenterology2005，11，48： | 29 |
| 4 | Shikonin induces glioma cell necroptosis in vitro by ROS overproduction and promoting RIP1/RIP3 necrosome formation显示文摘 | Bin LU Xu GONG Zong-qi WANG Ye DING Chen WANG Tian-fei LUO Mei-hua PIAO Fan-kai MENG Guang-fan CHI Yi-nan LUO Peng-fei GE | 2017 | Acta Pharmacologica Sinica2017，38，11： | 22 |
| 5 | The impacts of climate extremes on the terrestrial carbon cycle:A review显示文摘The increased frequency of climate extremes in recent years has profoundly affected terrestrial ecosystem functions and the welfare of human society. The carbon cycle is a key process of terrestrial ecosystem changes. Therefore, a better understanding and assessment of the impacts of climate extremes on the terrestrial carbon cycle could provide an important scientific basis to facilitate the mitigation and adaption of our society to climate change. In this paper, we systematically review the impacts of climate extremes(e.g. drought, extreme precipitation, extreme hot and extreme cold) on terrestrial ecosystems and their mechanisms. Existing studies have suggested that drought is one of the most important stressors on the terrestrial carbon sink, and that it can inhibit both ecosystem productivity and respiration. Because ecosystem productivity is usually more sensitive to drought than respiration, drought can significantly reduce the strength of terrestrial ecosystem carbon sinks and even turn them into carbon sources. Large inter-model variations have been found in the simulations of drought-induced changes in the carbon cycle, suggesting the existence of a large gap in current understanding of the mechanisms behind the responses of ecosystem carbon balance to drought, especially for tropical vegetation. The effects of extreme precipitation on the carbon cycle vary across different regions. In general, extreme precipitation enhances carbon accumulation in arid ecosystems, but restrains carbon sequestration in moist ecosystems. However, current knowledge on the indirect effects of extreme precipitation on the carbon cycle through regulating processes such as soil carbon lateral transportation and nutrient loss is still limited. This knowledge gap has caused large uncertainties in assessing the total carbon cycle impact of extreme precipitation. Extreme hot and extreme cold can affect the terrestrial carbon cycle through various ecosystem processes. Note that the severity of such climate extremes depends greatly on their timing, which needs to be investigated thoroughly in future studies. In light of current knowledge and gaps in the understanding of how extreme climates affect the terrestrial carbon cycle, we strongly recommend that future studies should place more attention on the long-term impacts and on the driving mechanisms at different time scales.Studies based on multi-source data, methods and across multiple spatial-temporal scales, are also necessary to better characterize the response of terrestrial ecosystems to climate extremes. | Shilong PIAO Xinping ZHANG Anping CHEN Qiang LIU Xu LIAN Xuhui WANG Shushi PENG Xiuchen WU | 2019 | Science China Earth Sciences2019，62，10： | 16 |
| 6 | Molecular Typing of Brucella Suis Collected from 1960s to 2010s in China by MLVA and PFGE显示文摘Brucellosis is a bacterial anthropozoonosis usually caused by Brucella abortus, Brucella melitensis, Brucella suis and Brucella canis. Brucella suis, the causative agent of swine brucellosis, is classified into five biovars and preferentially infects different animal hosts [1] . | LI Zhen Jun CUI Bu Yun CHEN Hai CHEN Jing Diao ZHAO Hong Yan PIAO Dong Ri JIANG Hai ZHANG Li TANG Xu KE Chang Wen YAO zhen TIAN Guo Zhong | 2013 | Biomedical and Environmental Sciences2013，26，6： | 13 |
| 7 | Exogenous pancreatic kininogenase protects against renal fibrosis in rat model of unilateral ureteral obstruction显示文摘Tissue kallikrein has protective function against various types of injury.In this study,we investigated whether exogenous pancreatic kininogenase(PK)conferred renoprotection in a rat model of unilateral ureteral obstruction(UUO)and H2O2-treated HK-2 cells in vitro.SD rats were subjected to UUO surgery,then PK(7.2 U/g per day,ip)was administered for 7 or 14 days.After the treatment,rats were euthanized;the obstructed kidneys were harvested for further examination.We found that PK administration significantly attenuated interstitial inflammation and fibrosis,and downregulated the expression of proinflammatory(MCP-1,TLR-2,and OPN)and profibrotic(TGF-β1 and CTGF)cytokines in obstructed kidney.UUO-induced oxidative stress,closely associated with excessive apoptotic cell death and autophagy via PI3K/AKT/FoxO1a signaling,which were abolished by PK administration.We further showed that PK administration increased the expression of bradykinin receptors 1 and 2(B1R and B2R)mRNA and the production of NO and cAMP in kidney tissues.Coadministration with either B1R antagonist(des-Arg9-[Leu8]-bradykinin)or B2R antagonist(icatibant)abrogated the renoprotective effects of PK,and reduced the levels of NO and cAMP in obstructed kidney.In H2O2-treated HK-2 cells,addition of PK(6 pg/mL)significantly decreased ROS production,regulated the expression of oxidant and antioxidant enzymes,suppressed the expression of TGF-β1 and MCP-1,and inhibited cell apoptosis.Our data demonstrate that PK treatment protects against the progression of renal fibrosis in obstructed kidneys. | Li-zhe Jin Hui-ying Li Jian Jin Shang-guo Piao Xiong-hu Shen Yan-ling Wu Jia-chong Xu Long-ye Zhang Yu-ji Jiang Hai-lan Zheng Ying-shun Jin Sheng Cui Kang Luo Yi Quan Can Li | 2020 | Acta Pharmacologica Sinica2020，41，12： | 9 |
| 8 | Clinical application of serial operations with preserving spleen显示文摘AIM: To evaluate the clinical application of serial operations with preservation of spleen.METHODS: Serial operations with preserving spleen were performed on 211 cases in our hospital from 1980 to 2000.The patient's age ranged from 13 to 56 years, averaging 3years. Diseases included splenic injury in 171 cases, portal hypertension in 9 cases, splenic cyst in 10 cases, and the lesion of pancreatic body and tail in 21 cases.RESULTS: All the cases were cured, and 129 patients were followedup from 3 months to 3 years with the leukocyte phagocytosis test, detection of immunoglubin, CT, 99mTc scanning and ultrasonogrsphy. The results were satisfactory.CONCLUSION: The operations with preserving spleen were safe, feasible, and worth of clinical application. | Hong-Chi Jiang~1 Bei Sun~1 Hai-Quan Qiao~1 Jun Xu~1 Da-Xun Piao~1 Hang Yin~2 1 Department of General Surgery,First Clinical Hospital,Harbin Medical University,Harbin 150001,China2 Department of General Surgery,Heilongjiang Provincial Hospital,Harbin 150001,China | 2001 | World Journal of Gastroenterology2001，7，6： | 8 |
| 9 | Bevacizumab plus capecitabine and cisplatin in Chinese patients with inoperable locally advanced or metastatic gastric or gastroesophageal junction cancer: randomized, double-blind, phase III study (AVATAR study)显示文摘 | Lin Shen Jin Li Jianming Xu Hongming Pan Guanghai Dai Shukui Qin Liwei Wang Jinwan Wang Zhenzhou Yang Yongqian Shu Ruihua Xu Lei Chen Yunpeng Liu Shiying Yu Lilian Bu Yongzhe Piao | 2015 | Gastric Cancer2015，，1： | 7 |
| 10 | Detection Rate, Distribution, Clinical and Pathological Features of Colorectal Serrated Polyps显示文摘 | Hai-Long Cao Xue Chen Shao-Chun Du Wen-Jing Song Wei-Qiang Wang Meng-Que Xu Si-Nan Wang Mei-Yu Piao Xiao-Cang Cao Bang-Mao Wang | 2016 | Chinese Medical Journal2016，，20： | 7 |
| 11 | Relationship between atrial natriuretic peptide-immunoreactive cells and microvessels in rat gastric mucosa显示文摘瞄准:调查极端结构的本地化在综合的伊朗的银币尿钠排泄的肽(ANP ) 在在老鼠胃粘膜的综合 ANP 房间和微容器之间的房间和关系。方法:嵌入免疫电子显微镜学技术的 Immunohistochemistry 技术和帖子被用来在胃粘膜关于综合 ANP 房间的表达式和综合 ANP 房间的极端结构的本地化验证调查结果。组织化学技术和鞣质的酸铁的氯化物方法(TA-Fe 染色方法) 被用来在胃的不同区域揭示微容器密度和综合 ANP 房间的分发。结果:表示 ANP 的房间是局部性的,综合 ANP 房间作为 enterochromaffin (EC ) 被识别在胃粘膜的房间。综合 ANP 房间在胃的不同区域存在。在粘膜的百分比综合 ANP 房间在身体(21.6%+/-3.6%) 在宫底(46.7%+/-5.3%) 最大、在窦(40.1%+/-4.5%) 中间、最少。在综合 ANP 房间的百分比和在窦粘膜,然而并非在宫底或身体粘膜的微容器的密度之间有一种积极关系。结论:ANP 被 EC 房间在老鼠胃粘膜综合,并且综合 ANP 房间在胃的宫底是很稠密的。ANP 可以在胃肠道地区性的 autocrine 或 paracrine 管理者充当不仅,而且作为内分泌的规章的肽。 | Chun-hui LI Zong-wei YANG Zheng-ri YIN Zheng JIN De-gang XING Lian-hua PIAO Yong-chul KIM Wen-xie XU | 2006 | Acta Pharmacologica Sinica2006，27，2： | 7 |
| 12 | Multi-locus variable-number tandem repeat analysis of Chinese Brucella strains isolated from 1953 to 2013显示文摘Background:Brucellosis was a common human and livestock disease caused by Brucella strains,the category B priority pathogens by the US Center for Disease Control(CDC).Identified as a priority disease in human and livestock populations,the increasing incidence in recent years in China needs urgent control measures for this disease but the molecular background important for monitoring the epidemiology of Brucella strains at the national level is still lacking.Methods:A total of 600 Brucella isolates collected during 60 years(from 1953 to 2013)in China were genotyped by multiple locus variable-number tandem repeat analysis(MLVA)and the variation degree of MLVA11 loci was calculated by the Hunter Gaston Diversity Index(HGDI)values.The charts and map were processed by Excel 2013,and cluster analysis and epidemiological distribution was performed using BioNumerics(version 5.1).Results:The 600 representative Brucella isolates fell into 104 genotypes with 58 singleton genotypes by the MLVA11 assay,including B.melitensis biovars 2 and 3(five main genotypes),B.abortus biovars 1 and 3(two main genotypes),B.suis biovars 1 and 3(three main genotypes),and B.canis(two main genotypes)respectively.While most B.suis biovar 1 and biovar 3 were respectively found in northern provinces and southern provinces,B.melitensis and B.abortus strains were dominant in China.Canine Brucellosis was only found in animals without any human cases reported.Eight Brucellosis epidemic peaks emerged during the 60 years between 1953 and 2013:1955-1959,1962-1969,1971-1975,1977-1983,1985-1989,1992-1997,2000-2008 and 2010-2013 in China.Conclusions:Brucellosis has its unique molecular epidemiological patterns with specific spatial and temporal distribution according to MLVA.Trial registration:IDOP-D-16-00101. | Guo-Zhong Tian Bu-Yun Cui Dong-Ri Piao Hong-Yan Zhao Lan-Yu Li Xi Liu Pei Xiao Zhong-Zhi Zhao Li-Qing Xu Hai Jiang Zhen-Jun Li | 2017 | Infectious Diseases of Poverty2017，6，1： | 6 |
| 13 | Maternal and Fetal Exposure to Four Carcinogenic Environmental Metals显示文摘Objective To examine maternal and fetal exposure levels to four carcinogenic metals,arsenic (As),cadmium (Cd),nickel (Ni),and beryllium (Be),and to investigate their environmental influences.Methods Metal concentrations in maternal and umbilical cord blood were measured by inductively coupled plasma-mass spectrometry (ICP-MS).Environmental factors that might play a role in exposure were analyzed using Mann-Whitney nonparametric U-tests and multiple linear regression.Results The concentrations of As,Cd,and Ni in umbilical cord blood (5.41,0.87,and 139.54 μg/L) were significantly lower than those in maternal blood (6.91,1.93,and 165.93 μg/L).There were significant positive correlations between the maternal and cord concentrations of each carcinogen.Our results showed that:(i) exposures to potentially harmful occupational factors during pregnancy were associated with high levels of maternal As,Cd,and Ni;(ii) living close to major transportation routes (<500 m) or exposure to second-hand smoke during pregnancy increased the maternal Cd levels and (iii) living close to industrial chimneys induced high maternal Ni levels.Multiple linear regression analysis showed that these environmental factors remained significant in models of the influences of these four carcinogens.Conclusion Both mothers and fetuses had been exposed to As,Cd,Ni,and Be.The increased levels of these carcinogens in pregnant women were associated with some detrimental environmental factors,such as occupational exposure,contact with second-hand smoke and living close to major transportation routes or industrial chimneys. | HUAI GUAN FENG-YUAN PIAO XIAO-WEI LI QIU-JUAN LI LEI XU KAZUHITO YOKOYAMA | 2010 | Biomedical and Environmental Sciences2010，23，6： | 5 |
| 14 | Monte Carlo simulation fused with target distribution modeling via deep reinforcement learning for automatic high-efficiency photon distribution estimation显示文摘Particle distribution estimation is an important issue in medical diagnosis. In particular, photon scattering in some medical devices extremely degrades image quality and causes measurement inaccuracy. The Monte Carlo(MC) algorithm is regarded as the most accurate particle estimation approach but is still time-consuming, even with graphic processing unit(GPU) acceleration. The goal of this work is to develop an automatic scatter estimation framework for high-efficiency photon distribution estimation. Specifically, a GPU-based MC simulation initially yields a raw scatter signal with a low photon number to hasten scatter generation. In the proposed method, assume that the scatter signal follows Poisson distribution, where an optimization objective function fused with sparse feature penalty is modeled. Then, an over-relaxation algorithm is deduced mathematically to solve this objective function. For optimizing the parameters in the over-relaxation algorithm, the deep Q-network in the deep reinforcement learning scheme is built to intelligently interact with the over-relaxation algorithm to accurately and rapidly estimate a scatter signal with the large range of photon numbers.Experimental results demonstrated that our proposed framework can achieve superior performance with structural similarity >0.94, peak signal-to-noise ratio >26.55 d B, and relative absolute error <5.62%, and the lowest computation time for one scatter image generation can be within 2 s. | Jianhui Ma Zun Piao Shuang Huang Xiaoman Duan Genggeng Qin Linghong Zhou Yuan Xu | 2021 | Photonics Research2021，9，3： | 5 |
| 15 | Molecular characteristics of Brucella melitensis isolates from humans in Qinghai Province, China显示文摘Background:The prevalence of human brucellosis in Qinghai Province of China has been increasing rapidly,with confirmed cases distributed across 31 counties.However,the epidemiology of brucellosis transmission has not been fully elucidated.To characterize the infecting strains isolated from humans,multiple-locus variable-number tandem repeats analysis(MLVA)and whole-genome single-nucleotide polymorphism(SNP)-based approaches were employed.Methods:Strains were isolated from two males blood cultures that were confirmed Brucella m elitensis positive following biotyping and MLVA.Genomic DNA was extracted from these two strains,and whole-genome sequencing was performed.Next,SNP-based phylogenetic analysis was performed to compare the two strains to 94 B.melitensis strains(complete genome and draft genome)retrieved from online databases.Results:The two Brucella isolates were identified as B.melitensis biovar 3(QH2019001 and QH2019005)following conventional biotyping and were found to have differences in their variable number tandem repeats(VNTRs)using MLVA-16.Phylogenetic examination assigned the 96 strains to five genotype groups,with QH2019001 and QH2019005 assigned to the same group,but different subgroups.Moreover,the QH2019005 strain was assigned to a new subgenotype,llj,within genotype II.These findings were then combined to determine the geographic origin of the two Brucella strains.Conclusions:Utilizing a whole-genome SNP-based approach enabled differences between the two B.m elitensis strains to be more clearly resolved,and facilitated the elucidation of their different evolutionary histories.This approach also revealed that QH2019005 is a member of a new subgenotype(Hj)with an ancient origin in the eastern Mediterranean Sea. | Zhi-Jun Zhao Ji-Quan Li Li Ma Hong-Mei Xue Xu-Xin Yang Yuan-Bo Zhao Yu-Min Qin Xiao-Wen Yang Dong-Ri Piao Hong-Yan Zhao Guo-Zhong Tian Qiang LiJian-Ling Wang Guang Tian Hai Jiang Li-Qing Xu | 2021 | Infectious Diseases of Poverty2021，10，2： | 5 |
| 16 | Integrative analysis of DNA methylation and gene expression identified cervical cancer-specific diagnostic biomarkers显示文摘Cervical cancer is the leading cause of death among women with cancer worldwide.Here,we performed an integrative analysis of Illumina HumanMethylation450K and RNA-seq data from TCGA to identify cervical cancer-specific DNA methylation markers.We first identified differentially methylated and expressed genes and examined the correlation between DNA methylation and gene expression.The DNA methylation profiles of 12 types of cancers,including cervical cancer,were used to generate a candidate set,and machine-learning techniques were adopted to define the final cervical cancer-specific markers in the candidate set.Then,we assessed the protein levels of marker genes by immunohistochemistry by using tissue arrays containing 93 human cervical squamous cell carcinoma samples and cancer-adjacent normal tissues.Promoter methylation was negatively correlated with the local regulation of gene expression.In the distant regulation of gene expression,the methylation of hypermethylated genes was more likely to be negatively correlated with gene expression,while the methylation of hypomethylated genes was more likely to be positively correlated with gene expression.Moreover,we identified four cervical cancer-specific methylation markers,cg07211381(RAB3C),cg12205729(GABRA2),cg20708961(ZNF257),and cg26490054(SLC5A8),with 96.2%sensitivity and 95.2%specificity by using the tenfold cross-validation of TCGA data.The four markers could distinguish tumors from normal tissues with a 94.2,100,100,and 100%AUC in four independent validation sets from the GEO database.Overall,our study demonstrates the potential use of methylation markers in cervical cancer diagnosis and may boost the development of new epigenetic therapies. | Wanxue Xu Mengyao Xu Longlong Wang Wei Zhou Rong Xiang Yi Shi Yunshan Zhang Yongjun Piao | 2019 | Signal Transduction and Targeted Therapy2019，4，1： | 4 |
| 17 | The role of bone marrow-derived cells in the origin of liver cancer revealed by single-cell sequencing显示文摘Objective:Epithelial cancers often originate from progenitor cells,while the origin of hepatocellular carcinoma(HCC)is still controversial.HCC,one of the deadliest cancers,is closely linked with liver injuries and chronic inflammation,which trigger massive infiltration of bone marrow-derived cells(BMDCs)during liver repair.Methods:To address the possible roles of BMDCs in HCC origination,we established a diethylnitrosamine(DEN)-induced HCC model in bone marrow transplanted mice.Immunohistochemistry and frozen tissue immunofluorescence were used to verify DENinduced HCC in the pathology of the disease.The cellular origin of DEN-induced HCC was further studied by single cell sequencing,single-cell nested PCR,and immunofluorescence-fluorescence in situ hybridization.Results:Studies by using single cell sequencing and biochemical analysis revealed that HCC cells in these mice were coming from donor mice BMDCs,and not from recipient mice.Furthermore,the copy numbers of mouse orthologs of several HCC-related genes previously reported in human HCC were also altered in our mouse model.DEN-induced HCCs exhibited a similar histological phenotype and genomic profile as human HCCs.Conclusions:These results suggested that BMDCs are an important origin of HCC,which provide important clues to HCC prevention,detection,and treatments. | Lu Chen Xianfu Yi Piao Guo Hua Guo Ziye Chen Chunyu Hou Lisha Qi Yongrong Wang Chengwen Li Peng Liu Yucun Liu Yuanfu Xu Ning Zhang | 2020 | Cancer Biology & Medicine2020，17，1： | 4 |
| 18 | Pingchuan formula(平喘方) improves allergic asthma in mice through inhibiting nuclear factor-kappa B/mitogen-activated protein kinase signaling pathway显示文摘OBJECTIVE:To examine the role and decipher the mechanism of Pingchuan formula(平喘方,PCF)in treating allergic asthma.METHODS:The mice were treated with saline,dexamethasone(DXM)and PCF for 1 week after the asthma model was established and their respiratory function including respiratory resistance(RI),pulmonary dynamic compliance(Cdyn)and maximum voluntary ventilation(MVV)were measured.In addition,cellular changes in bronchoalveolar lavage fluid(BALF)and pathological changes in lung biopsy as well as the expression level ofα-smooth muscle actin(α-SMA),transforming growth factor-beta1(TGF-β1)in BALF and interleukin-5(IL-5),interleukin-13(IL-13),tumor necrosis factor-α(TNF-α),interferon-γ(IFN-γ),nuclear factor-kappa B-p65(NF-κBp65),inhibitor-αof nuclear transcription factorκB(IκBα),p38 mitogen-activated protein kinase(p38 MAPK),c-jun n-terminal kinase(JNK)and its phosphorylated proteins in lung tissue were also examined and compared among different groups.RESULTS:Our data suggested that the respiratory functions were significantly improved and the pathological changes ameliorated in the DXM group and the PCF group compared to the model group.Both DXM and PCF effectively decreased the number of eosinophils,lymphocytes,and neutrophils in BAL as well as the secretion ofα-SMA and TGF-β1,IL-5,IL-13,while increased the expression of TNF-αand IFN-γ.Furthermore,our study indicated that the NF-κBp65,IκBα,p38 MAPK and JNK pathways were inhibited under the treatment of PCF.CONCLUSION:Our data indicated that PCF can attenuate the inflammatory response in asthma through inhibiting the NF-κB/MAPK signaling pathway.This study not only supported the use of PCF in allergic asthma in clinic but also shed light upon afurther understanding of the disease pathogenesis. | WU Mingyun YU Jianer BAI Li XUE Zheng JIANG Shenhua LI Liqing PIAO Xiang XU Wanchao WANG Jiani SHEN Qian | 2021 | Journal of Traditional Chinese Medicine2021，41，6： | 4 |
| 19 | Association between Adiponectin and Metabolic Syndrome in Older Adults from Major Cities of China显示文摘Objective To investigate the association between adiponectin and metabolic syndrome (MetS) and related diseases in older adults from major cities of China.Methods A total of 2 049 adults at the age of 60-96 years from 18 major cities of China were enrolled in the study.Plasma adiponectin and insulin concentrations were measured.Insulin resistance was assessed by homeostasis model assessment of insulin resistance (HOMA-IR).The definitions proposed by International Diabetes Federation (IDF)and American Heart Association/National Heart,Lung,and Blood Institute (AHA/NLHBI)were used to identify MetS.Results The adiponectin concentration increased with the advance of age and was higher in women than in men.The sex specific adiponectin concentration was inversely correlated with body mass index(BMI),waist circumference,diastolic blood pressure,triglycerides,glucose and fasting blood insulin,and positively correlated with HDL-C(P<0.001).The adiponectin concentration decreased with increasing MetS components.Compared with the 4th sex-specific adiponectin quartile,the odds ratio(OR)for prevalent MetS-IDF and MetS-AHA/NLHBI in subjects of the 1st quartile group was 3.25(95% CI:2.24,4.71)and 3.21(95% CI:2.26,4.55),respectively.The association was independent of age,sex,life-style factors,medication,family history of chronic diseases,BMI,and HOMA-IR.The OR for MetS was much higher than those of MetS components and its related diseases.Conclusion Adiponectin is strongly associated with MetS independent of insulin resistance and obesity in older adults from major cities in China.The adiponectin concentration is a useful predictor for the risk of MetS. | QIN ZHUO ZHI-QING WANG PING FU JIAN-HUA PIAO YUAN TIAN JIE XU XIAO-GUANG YANG | 2010 | Biomedical and Environmental Sciences2010，23，1： | 4 |
| 20 | Intake of Vitamin A,Thiamine,Riboflavin,Vitamin C,and Niacin Among Children Aged 6−11 Years Old—China,2016−2017显示文摘Summary What is already known on this topic?Insufficient intake of vitamins is one of the major nutritional problems in children aged 6-11 years old in China,and the problem is particularly severe for rural populations.What is added by this report?Among children aged 6-11 years old in 2016-2017,the average intakes of vitamin A,thiamine. | Wei Piao Lahong Ju Hongyun Fang Qiya Guo Shuya Cai Xiaoli Xu Shujuan Li Xue Cheng Liyun Zhao Dongmei Yu | 2021 | China CDC weekly2021，3，11： | 3 |
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