|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Fusing T5 exonuclease with Cas9 and Cas12a increases the frequency and size of deletion at target sites显示文摘CRISPR/Cas systems, especially CRISPR/Cas9, generally result in small insertions/deletions, which are unlikely to eliminate the functions of regulatory and other non-coding sequences. To generate larger genomic deletions usually requires the use of pairs of guide RNAs. Here we show that it is possible to create such deletions with a single guide RNA by fusing Cas9 or Cas12a with T5 exonuclease(T5exo). These fusion constructs were found to increase both the frequency and size of deletions at target loci in rice protoplasts and seedlings. Moreover, the genome editing efficiencies of Cas9 and Cas12a were also enhanced by fusion with T5 exonuclease. These T5exo-Cas fusions expand the CRISPR toolbox, and facilitate knockout of regulatory and non-coding DNA sequences. From a wider standpoint, our results suggest a general strategy for producing larger deletions using other Cas nucleases. | Qianwei Zhang Kangquan Yin Guanwen Liu Shengnan Li Mengou Li Jin-Long Qiu | 2020 | Science China(Life Sciences)2020，63，12： | 6 |
| 2 | Effect of Hydrological Connectivity on Soil Carbon Storage in the Yellow River Delta Wetlands of China显示文摘Hydrological connectivity has significant effects on the functions of estuarine wetland ecosystem.This study aimed to examine the dynamics of hydrological connectivity and its impact on soil carbon pool in the Yellow River Delta,China.We calculated the hydrological connectivity based on the hydraulic resistance and graph theory,and measured soil total carbon and organic carbon under four different hydrological connectivity gradients(Ⅰ0‒0.03,Ⅱ0.03‒0.06,Ⅲ0.06‒0.12,Ⅳ0.12‒0.39).The results showed that hydrological connectivity increased in the north shore of the Yellow River and the south tidal flat from 2007 to 2018,which concentrated in the mainstream of the Yellow River and the tidal creek.High hydrological connectivity was maintained in the wetland restoration area.The soil total carbon storage and organic carbon storage significantly increased with increasing hydrological connectivity fromⅠtoⅢgradient and decreased inⅣgradient.The highest soil total carbon storage of 0‒30 cm depth was 5172.34 g/m^(2),and organic carbon storage 2764.31 g/m^(2)inⅢgradient.The hydrological connectivity changed with temporal and spatial change during 2007‒2018 and had a noticeable impact on soil carbon storage in the Yellow River Delta.The results indicated that appropriate hydrological connectivity,i.e.0.08,could effectively promote soil carbon storage. | FENG Jiuge LIANG Jinfeng LI Qianwei ZHANG Xiaoya YUE Yi GAO Junqin | 2021 | Chinese Geographical Science2021，31，2： | 4 |
| 3 | Rapid detection of organophosphorus pesticide residue on Prussian blue modified dual-channel screen-printed electrodes combing with portable potentiostat显示文摘A novel electrochemical method for the rapid detection of organophosphorus pesticide residues was realized on a dual-channel screen-printed electrode(DSPE) that was integrated with a portable smartphone-controlled potentiostat. The two carbon working channels of DSPE were first modified by electrodepositing of Prussian blue. The channels were then modified with acetylcholinesterase(AChE)via Nafion. The inhibition ratio of AChE was detected by comparing the electrical current of acetylthiocholine(ATCh) that was catalyzed by the enzyme electrodes with(channel 1) and without(channel 2) organophosphorus pesticide. Inhibition ratios were related with the negative logarithm of the organophosphorus pesticide(trichlorfon, oxamyl, and isocarbophos) concentrations at optimum experimental conditions(pH 6.9 of electrolyte, 0.2 V working potential, 2.5 μL AChE modification amount, and 15 min inhibition time). The linear equations were I% = 32.301 gC +253.3(R = 0.9750) for isocarbophos, 1% = 35.991 gC + 270.1(R = 0.9668) for chlorpyrifos, and 1% = 33.701 gC+ 250.5(R = 0.9606) for trichlorfon. The detection limits were calculated as 10^(-7)g/mL. Given that the inhibition ratios were only related with pesticide concentration and not with pesticide species, the proposed electrodes and electrometer can rapidly detect universal organophosphorus pesticides and assess pesticide pollution. | Qianwei Shi Yuanjie Teng Yuchao Zhang Wenhan Liu | 2018 | Chinese Chemical Letters2018，29，9： | 4 |
| 4 | Relationship between COX-2 and cell cycle-regulatory proteins in patients with esophageal squamous cell carcinoma显示文摘AIM: To investigate the correlation between cyclooxygenase-2 (COX-2) and cell cycle-regulatory proteins in patients with esophageal squamous cell carcinoma (ESCC). METHODS: One hundred and two surgically obtained specimens of ESCC were randomly collected. All specimens were obtained from patients who had not received chemoor radiotherapy prior to surgical resection.Twenty-eight specimens of normal squamous epithelium served as controls. The expression of COX-2, Ki-67, cyclin A and p27 was examined by immunohistochemistry. The Pearson test was used to analyze the relationship between groups. RESULTS: The protein level of COX-2, Ki-67 and cyclin A was significantly higher in ESCC than in normal squamous epithelium (74.7±61.2 vs 30.2 ± 43.4, 64.0 ± 51.6 vs 11.6 ± 2.3, 44.2 ± 32.2 vs 11.7 ± 5.0, respectively, all P<0.01). In contrast, the protein level of p27 was signifi cantly lower in ESCC than in normal squamous epithelium (182.0 ±69.0 vs 266.4±28.0, P<0.01). In ESCC, COX-2 expression was correlated with T stage, the score of T1-T2 stage was lower than that of T3-T4 stage (55.0±42.3 vs 83.0 ± 66.5, P<0.05), and Ki-67, cyclin A and p27 expressions were correlated with the tumor differentiation (43.8±31.7 vs 98.4± 84.8, 32.0 ± 19.0 vs 54.1 ±53.7,206.2±61.5 vs 123.5±68.3, respectively, all P<0.01). COX-2 expression was positively correlated to Ki-67, cyclin A and negatively correlated to p27 expression in ESCC (r=0.270, 0.233 and-0.311, respectively, all P<0.05).CONCLUSION: The expression of COX-2 is correlated with tumor cell invasion and is closely related to the cell proliferation in patients with ESCC. | Jun-Xing Huang Mao-Song Lin Yun-Lei Zhang Feng-Yue Li Rong-Yu Qian Wei-Chang Chen Zheng-Xiang Song Ping Chen Eeva Salminen | 2010 | World Journal of Gastroenterology2010，16，47： | 3 |
| 5 | Enhanced removal of sulfonamide antibiotics from water by phosphogypsum modified biochar composite显示文摘Antibiotic pollution has become a global eco-environmental issue.To reduce sulfonamide antibiotics in water and improve resource utilization of solid wastes,phosphogypsummodified biochar composite(PMBC)was prepared via facile one-step from distillers grains,wood chips,and phosphogypsum.The physicochemical properties of PMBCwere characterized by scanning electron microscope(SEM),Fourier transform infrared spectroscopy(FTIR),Zeta potential,X-ray diffraction(XRD),etc.The influencing factors,adsorption behaviors,and mechanisms of sulfadiazine(SD)and sulfamethazine(SMT)onto PMBC were studied by batch and fixed bed column adsorption experiments.The results showed that the removal rates of SD and SMT increased with the increase of phosphogypsum proportion,while decreased with the increase of solution pH.The maximum adsorption capacities of modified distillers grain and wood chips biochars for SD were 2.98 and 4.18 mg/g,and for SMT were 4.40 and 8.91mg/g,respectively,which was 9.0–22.3 times that of pristine biochar.Fixed bed column results demonstrated that PMBC had good adsorption capacities for SD and SMT.When the solution flow rate was 2.0 mL/min and the dosage of PMBC was 5.0 g,the removal rates of SD and SMT by modified wood chips biochar were both higher than 50%in 4 hr.The main mechanisms of SD and SMT removal by PMBC are hydrogen bonding,π-πdonor-acceptor,electrostatic interaction,and hydrophobic interaction.This study provides an effective method for the removal of antibiotics in water and the resource utilization of phosphogypsum. | Zonghong Jiang Miao Chen Xinqing Lee Qianwei Feng Ning Cheng Xueyang Zhang Shengsen Wang Bing Wang | 2023 | Journal of Environmental Sciences2023，，8： | 2 |
| 6 | A study on theoretical calculation method of subway safety evaeuation显示文摘 | Zhang Bing Xu Zhisheng Zhao Qianwei | 2014 | Procedia Engi neering2014，71，： | 1 |
| 7 | LEAKAGE-PLUGGING WHILE DRILLING AND PLUGGING AGENTS显示文摘LEAKAGE-PLUGGINGWHILEDRILLINGANDPLUGGINGAGENTSPengZhenbinZhangXuzhiDaiQianwei(DepartmentofGeology,CentralSouthUniversityofTec... | Peng Zhenbin Zhang Xuzhi Dai Qianwei(Department of Geology, Central South University ofTechnology, Changsha 410083, China) | 1996 | Journal of Central South University1996，8，1： | 1 |
| 8 | A study on theoretical calculation method of subway safety evacuation显示文摘 | ZHANG Bing XU Zhisheng ZHAO Qianwei | 2014 | Procedia Engineering2014，71，： | 1 |
| 9 | Point-cloud segmentation of individual trees in complex natural forest scenes based on a trunk-growth method显示文摘Forest resource management and ecological assessment have been recently supported by emerging technologies.Terrestrial laser scanning(TLS)is one that can be quickly and accurately used to obtain three-dimensional forest information,and create good representations of forest vertical structure.TLS data can be exploited for highly significant tasks,particularly the segmentation and information extraction for individual trees.However,the existing single-tree segmentation methods suffer from low segmentation accuracy and poor robustness,and hence do not lead to satisfactory results for natural forests in complex environments.In this paper,we propose a trunk-growth(TG)method for single-tree point-cloud segmentation,and apply this method to the natural forest scenes of Shangri-La City in Northwest Yunnan,China.First,the point normal vector and its Z-axis component are used as trunk-growth constraints.Then,the points surrounding the trunk are searched to account for regrowth.Finally,the nearest distributed branch and leaf points are used to complete the individual tree segmentation.The results show that the TG method can effectively segment individual trees with an average F-score of 0.96.The proposed method applies to many types of trees with various growth shapes,and can effectively identify shrubs and herbs in complex scenes of natural forests.The promising outcomes of the TG method demonstrate the key advantages of combining plant morphology theory and LiDAR technology for advancing and optimizing forestry systems. | Qianwei Liu Weifeng Ma Jianpeng Zhang Yicheng Liu Dongfan Xu Jinliang Wang | 2021 | Journal of Forestry Research2021，32，6： | 1 |
| 10 | BCAT1 promotes lung adenocarcinoma progression through enhanced mitochondrial function and NF-κB pathway activation显示文摘Lung cancer is one of the most prevalent and malignant cancers,among which lung adenocarcinoma(LUAD)accounts for the majority and remains a major cause of cancer-related mortality worldwide(Cui et al.,2019).Despite the growing intensity of research on the pathobiology and progression of lung cancer and the fact that many genes have been identified as potential drivers and targets for therapy(Luo et al.,2019;Zhang et al.,2019),the treatment and prognosis of lung cancer patients have hardly improved.Therefore,this study aimed to investigate the precise mechanism of lung cancer development and explore efficient diagnostic and therapeutic methods for clinical treatment. | Mengdan YU Qianwei ZHAO Jinxia LI Fang XU Zhibiao ZHANG Yixian LIU Liping DAI Bingxia ZHANG Jianying ZHANG Jintao ZHANG | 2022 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2022，23，9： | 0 |
| 11 | In-situ coupling construction of interface bridge to enhance electrochemical stability of all solid-state lithium metal batteries显示文摘Polymer-based composite electrolytes composed of three-dimensional Li_(6.4)La_(3)Zr_(2)Al_(0.2)O_(12)(3D-LLZAO)have attracted increasing attention due to their continuous ion conduction and satisfactory mechanical properties.However,the organic/inorganic interface is incompatible,resulting in slow lithium-ion transport at the interface.Therefore,the compatibility of organic/inorganic interface is an urgent problem to be solved.Inspired by the concept of“gecko eaves”,polymer-based composite solid electrolytes with dense interface structures were designed.The bridging of organic/inorganic interfaces was established by introducing silane coupling agent(3-chloropropyl)trimethoxysilane(CTMS)into the PEO-3D-LLZAO(PL)electrolyte.The in-situ coupling reaction improves the interface affinity,strengthens the organic/inorganic interaction,reduces the interface resistance,and thus achieves an efficient interface ion transport network.The prepared PEO-3D-LLZAO-CTMS(PLC)electrolyte exhibits enhanced ionic conductivity of 6.04×10^(-4)S cm^(-1)and high ion migration number(0.61)at 60℃and broadens the electrochemical window(5.1 V).At the same time,the PLC electrolyte has good thermal stability and high mechanical properties.Moreover,the Li Fe PO_(4)|PLC|Li battery has excellent rate performance and cycling stability with a capacity decay rate of 2.2%after 100 cycles at 60℃and 0.1 C.These advantages of PLC membranes indicate that this design approach is indeed practical,and the in-situ coupling method provides a new approach to address interface compatibility issues. | Qianwei Zhang Rong Yang Chao Li Lei Mao Bohai Wang Meng Luo Yinglin Yan Yiming Zou Lisheng Zhong Yunhua Xu | 2024 | Journal of Energy Chemistry2024，89，2： | 0 |
| 12 | Effect of Cl doping amount on the microstructure,photovoltaic properties and ferroelectric properties of Bi-based lead-free perovskite显示文摘Organic-inorganic hybrid perovskites with 3D perovskite structure have gained much attention as light harvesting materials in thinfilm photovoltaics.This is because of their outstanding light-absorption characteristics,charge-transport dynamics and their simple processability using lab-scale solution and vapor phase deposition techniques.However,the inherent instability and lead toxicity of lead-based PSCs are the major problems at present.Recent studies have shown that the(CH_(3)NH_(3)T_(3)Bi_(2)I_(9)(MBI)0D bismuth-based compound can be used as an optical absorption layer in solar cells.In this paper,the(CH_(3)NH_(3)T_(3)Bi_(2)I_(9)was doped with Cland a series of(CH_(3)NH_(3)T_(3)Bi_(2)I_(9-x)Cl_(x)films were prepared.The effects of different doping amounts on the microstructure,photovoltaic properties and ferroelectric properties were systematically investigated.Scanning electron microscope(SEM)and Atomic force microscope(AFM)analysis showed that with the increase of doping content,the density of the films increased and the roughness decreased.The photoelectric conversion efficiency of(CH_(3)NH_(3)T_(3)Bi_(2)I_(9-x)Cl_(x)raises with the increase of doping content.For example,the photoelectric conversion efficiency of(C_(H)3NH_(3)T_(3)Bi_(2)I_(3)Cl_(6)is 0.473%.We find that the leakage current descends into the increase in doping content,which may be due to the increase in the film density and the decrease of porosity.These research results have a positive effect on the development of Bi-based lead-free perovskite. | Shilong Zhang Ruicheng Xu Zhendong Li Qianwei Zhang Li Cheng Zhenhua Wang Chunlin Fu | 2019 | Journal of Advanced Dielectrics2019，9，2： | 0 |
| 13 | Design and synthesis of novel α-aminoamides derivatives as Nav1.7 inhibitors for antinociception显示文摘Three novel series of α-aminoamides derivatives were designed and synthesized based on ralfinamide,and their Nav1.7 inhibitory activities were evaluated using manual patch clamp electrophysiology. Active compounds inhibited Nav1.7 with half maximal inhibitory concentration(IC_(50)) values ranging from2.9 μmol/L to 21.4 μmol/L. Among them, the most potent compound 19h exhibited about 12-fold potency better than ralfinamide. The investigation of their structure-activity relationship gives a strategy to improve the Nav1.7 inhibition of ralfinamide analogues. Compound 19h was efficacious in antinociception in the mouse spared nerve injury(SNI) model of neuropathic pain without causing sedation in the open field test. | Dengqi Xue Yani Liu Yilin Zheng Heling Niu Liying Dong Xiangshuo Ouyang Siyu Song Denggao Zhang Qianwei Ge Kewei Wang Liming Shao | 2022 | Chinese Chemical Letters2022，33，3： | 0 |
| 14 | BnbHLH92a negatively regulates anthocyanin and proanthocyanidin biosynthesis in Brassica napus显示文摘Yellow seed trait is a desirable characteristic with potential for increasing seed quality and commercial value in rapeseed,and anthocyanin and proanthocyanidins(PAs)are major seed-coat pigments.Few transcription factors involved in the regulation of anthocyanin and PAs biosynthesis have been characterized in rapeseed.In this study,we identified a transcription factor gene BnbHLH92a(BnaA06T0441000ZS)in rapeseed.Overexpressing BnbHLH92a both in Arabidopsis and in rapeseed reduced levels of anthocyanin and PAs.Correspondingly,the expression profiles of anthocyanin and PA biosynthesis genes(TT3,BAN,TT8,TT18,and TTG1)were shown by quantitative real-time PCR to be inhibited in BnbHLH92a-overexpressing Arabidopsis seeds,indicating that BnbHLH92a represses the anthocyanin and PA biosynthesis pathway in Arabidopsis.BnbHLH92a physically interacts with the BnTTG1 protein and represses the biosynthesis of anthocyanins and PAs in rapeseed.BnbHLH92a also binds directly to the BnTT18 promoter and represses its expression.These results suggest that BnbHLH92a is a novel upstream regulator of flavonoid biosynthesis in B.napus. | Ran Hu Meichen Zhu Si Chen Chengxiang Li Qianwei Zhang Lei Gao Xueqin Liu Shulin Shen Fuyou Fu Xinfu Xu Ying Liang Liezhao Liu Kun Lu Hao Yu Jiana Li Cunmin Qu | 2023 | The Crop Journal2023，11，2： | 0 |
| 15 | Natural forest ALS-TLS point cloud data registration without control points显示文摘Airborne laser scanning(ALS)and terrestrial laser scanning(TLS)has attracted attention due to their forest parameter investigation and research applications.ALS is limited to obtaining fi ne structure information below the forest canopy due to the occlusion of trees in natural forests.In contrast,TLS is unable to gather fi ne structure information about the upper canopy.To address the problem of incomplete acquisition of natural forest point cloud data by ALS and TLS on a single platform,this study proposes data registration without control points.The ALS and TLS original data were cropped according to sample plot size,and the ALS point cloud data was converted into relative coordinates with the center of the cropped data as the origin.The same feature point pairs of the ALS and TLS point cloud data were then selected to register the point cloud data.The initial registered point cloud data was fi nely and optimally registered via the iterative closest point(ICP)algorithm.The results show that the proposed method achieved highprecision registration of ALS and TLS point cloud data from two natural forest plots of Pinus yunnanensis Franch.and Picea asperata Mast.which included diff erent species and environments.An average registration accuracy of 0.06 m and 0.09 m were obtained for P.yunnanensis and P.asperata,respectively. | Jianpeng Zhang Jinliang Wang Feng Cheng Weifeng Ma Qianwei Liu Guangjie Liu | 2023 | Journal of Forestry Research2023，34，3： | 0 |
| 16 | Biomineralized nanoparticles for the immobilization and degradation of crude oil-contaminated soil显示文摘Accidental oil leaks and spills often cause server soil pollution,and in situ remediation is a powerful and economical treatment technology.While during in situ remediation process,unpredicted migration of petroleum hydrocarbon in heterogeneous soil will lead to a long-term source of persistent aquifer contamination.To reduce the migration of petroleum hydrocarbon and effectively improve the in situ remediation efficiency,herein,fungal biomineralization strategy was proposed for the immobilization of petroleum contaminants.A ureolytic fungi strain with crude oil-degradation ability was screened and identified as Chaetomium globosum.When incubated in medium containing Ca2+and crude oil,a mineral corona with spiny nanoparticles was formed at the edge of oil and the interface characters were analyzed using fluorescent pH and dissolved oxygen(DO)sensing films,respectively.Results indicated that biominerals preferred to aggregate around the edge of crude oil,providing favorable microenvironment for fungal growth and then leading to the increase of pH in the microenvironment,eventually accompanied by the formation of mineral corona.The mineral corona with numerous nanoparticles may act as a solid and stable shell,limiting or reducing the mobility of crude oil,and providing enough time for fungal biodegradation.After 28 days incubation,oilcontaminated soil treated with fungal biomineralization showed better immobilization ability for total petroleum hydrocarbon(TPH)under simulated acid-rain condition and higher TPH removal efficiency.This is the first demonstration for the immobilization of oil through fungal biomineralized nanoparticles,thus providing a novel strategy for the in situ remediation of oilcontaminated sites. | Daoqing Liu Qianwei Li Enhui Liu Miao Zhang Jicheng Liu Chunmao Chen | 2023 | Nano Research2023，16，10： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员