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| 1 | Development of cell-based pseudovirus entry assay to identify potential viral entry inhibitors and neutralizing antibodies against SARS-CoV-2显示文摘Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is the causative virus of the coronavirus disease 2019(COVID-19)pandemic.To establish a safe and convenient assay system for studying entry inhibitors and neutralizing antibodies against SARS-CoV-2,we constructed a codon-optimized,full-length C-terminal mutant spike(S)gene of SARS-CoV-2.We generated a luciferase(Luc)-expressing pseudovirus containing the wild-type or mutant S protein of SARS-CoV-2 in the envelope-defective HIV-1 backbone.The key parameters for this pseudovirus-based assay,including the S mutants and virus incubation time,were optimized.This pseudovirus contains a Luc reporter gene that enabled us to easily quantify virus entry into angiotensin-converting enzyme 2(ACE2)-expressing 293T cells.Cathepsin(Cat)B/L inhibitor E64d could significantly block SARS-CoV-2 pseudovirus infection in 293T-ACE2 cells.Furthermore,the SARS-CoV-2 spike pseudotyped virus could be neutralized by sera from convalescent COVID-19 patients or recombinant ACE2 with the fused Fc region of human IgG1.Thus,we developed a pseudovirus-based assay for SARS-CoV-2,which will be valuable for evaluating viral entry inhibitors and neutralizing antibodies against this highly pathogenic virus. | Jie Hu Qingzhu Gao Changlong He Ailong Huang Ni Tang Kai Wang | 2020 | Genes & Diseases2020，7，4： | 15 |
| 2 | Grassland degradation in Northern Tibet based on remote sensing data显示文摘This study selected vegetation cover as the main evaluation index, calculated the grassland degradation index (GDI) and established the remote sensing monitoring and evaluation system for grassland degradation in Northern Tibet, according to the National Standard (GB19377-2003), based on the remote sensing data such as NDVI data derived from NOAA/AVHRR with a spatial resolution of 8 km of 1981-2000, from SPOT/VGT with a spatial resolution of 1 km of 2001 and from MODIS with a spatial resolution of 0.25 km of 2002-2004 respectively in this area, in combination with the actual condition of grassland degradation. The grassland degradation processes and their responses to climate change during 1981-2004 were discussed and analyzed in this paper. The result indicated that grassland degradation in Northern Tibet is very serious, and the mean value of GDI in recent 20 years is 2.54 which belongs to the serious degradation grade. From 1981 to 2004, the GDI fluctuated distinctly with great interannual variations in the proportion of degradation degree and GDI but the general tendency turned to severe-grade during this period with the grassland degradation grade changed from light degraded to serious degraded in Northern Tibet. The extremely serious degraded and serious degraded grassland occupied 1.7% and 8.0% of the study area, the moderate and light degraded grassland accounted for 13.2% and 27.9% respectively, and un-degraded grassland occupied 49.2% of the total grassland area in 2004. The grassland degradation was serious, especially in the conjunctive area of Naqu, Biru and Jiali counties, the headstream of the Yangtze River lying in the Galadandong snow mountain and glaciers, the area along the Qinghai-Tibet highway and railway, and areas around the Tanggula and Nianqingtanggula snow mountains and glaciers. So the snow mountains and glaciers as well as their adjacent areas in Northern Tibet were sensitive to climate change and the areas along the vital communication line with frequent human activities experienced relatively serious grassland degradation. | GAO Qingzhu LI Yu'e WAN Yunfan LIN Erda XIONG Wei JIANGCUN Wangzha WANG Baoshan LI Wenfu | 2006 | Journal of Geographical Sciences2006，16，2： | 10 |
| 3 | Size Distributions of Aerosol Sulfates and Nitrates in Beijing during the 2008 Olympic Games: Impacts of Pollution Control Measures and Regional Transport显示文摘为 2008 个奥运会,激烈的控制措施在二氧化物(SO2 ) ,氮氧化物(没有 x ) 和处理穷人的问题的另外的污染物质通风的硫的工业、城市的排出物上被实现在北京的质量。在微粒硫酸盐和硝酸盐集中以及他们的尺寸分布上调查 SO2 和没有 x 减小的效果,分离尺寸的喷雾器样品在照原尺寸的控制前后在北京在城市、顺风的农村地点用微孔的一致存款 impactors (MOUDI ) 被收集。在采样时期期间,在城市、农村的地点的好粒子(PM1.8 ) 的集体集中分别地是 94.0 和 85.9 g m3。超过 90% 硫酸盐和 60% 硝酸盐形成了同样好的粒子。得益于有益的气象学的条件和来源控制,硫酸盐在奥林匹克期间在相当低的集中并且首先在冷凝作用模式被观察。控制措施的效果独立被分析让北风和南方的 air-mass-dominated 天说明任何偏爱。在控制措施被实现以后,当在北方的空气群众占优势时,下午,硫酸盐,和硝酸盐显著地被减少,与在更大的粒子的减小的一个更高的百分比。在控制措施被实现以后,微滴模式粒子,在控制被实现以前,统治了硫酸盐和硝酸盐,显著地在集体集中被减少。但当弄脏的南方的空气群众占优势时,本地来源控制在北京测量,有效地没从北方中国平原由于庞大的地区性的贡献减少周围的硫酸盐集中。 | WANG Xinfeng WANG Tao Ravi Kant PATHAK Mattias HALLQUIST GAO Xiaomeit NIE Wei XUE Likun GAO Jian GAO Rui ZHANG Qingzhu WANG Wenxing WANG Shulan CHAI Fahe CHEN Yizhen | 2013 | Advances in Atmospheric Sciences2013，30，2： | 8 |
| 4 | Rice re-cultivation in southern China: An option for enhanced climate change resilience in rice production显示文摘在南部的中国种的瑞斯说明在播种面积的 94% 总数和在生产的 88% 总数,它非常与中国食物有关系安全。由于在在空间 / 时间的分发的水可获得性和米饭生长之间的延长冲突,然而,米饭生产在 16%22% 的面积受不了季节的干旱,它损害食物生产能力和食物安全。与米饭和惯例集中于季节的干旱的空间分发适应它,并且基于平衡的水的分析供应为并且在 19812030 期间在成长季节规模由米饭要求,这份报纸估计变化在在变化气候下面的米饭生产的过程的季节的干旱以后,并且鉴别为气候的一般米饭重新耕作选择改变改编。一些结论能如下被得出。( 1 )瑞斯在季节的干旱建议衰落,与早季节米饭(早米饭此后)由 12,500 km2 ,由 80,000 km2 的中间的季节米饭(中间的米饭),和由 25,000 km2 的特别地迟了的季节米饭(迟了的米饭),它占几乎20%它的栽培面积。由于气候变化,在生产区域的主要米饭的季节的干旱趋于一般来说减轻,这被显示,迟了的季节米饭特别地。(2 ) 未来气候变化在在中国生产区域的米饭在水资源的空间 / 时间的分发上引起重要影响。为 rice-re-cultivation 基于预先设计的改编行动,米饭耕作模式经历在 19812000 和 20012030 之间的重要改变。在东方贵州和西方的湖南,单个早正的单个干燥耕作的模式被变成两倍收割。在东方湖南,干燥收割的模式加单个干燥耕作早被变成单身者。在北安徽,收割的干燥耕作的模式被变成中间的米饭。所有这些以后为米饭生产针对水的潜在的足够的可获得性。(3 ) 在这份报纸开发的瑞斯重新耕作模式帮助重新平衡对使用空间分析工具使米饭生长适应变化的米饭生长的水需求和供应从空间观点浇可获得性,并且找到响应气候的制片人友好的重新耕作选择改变的米饭。 | MA Xin WU Shaohong LI Yu'e ZHANG Xueyan GAO Qingzhu WU Yang | 2013 | Journal of Geographical Sciences2013，23，1： | 5 |
| 5 | Overexpression of the neuroglobin gene delivered by ultrasound-targeted microbubble destruction protects SH-SY5Y cells against cobalt chloride induced hypoxia显示文摘In this study, we examined the effects of neuroglobin gene (Ngb) transfection into SH-SY5Y cells, using ultrasound-targeted microbubble destruction (UTMD), on cobalt chloride-induced hypoxia. With an ultrasound intensity of 0.8 W/cm2, a 60-second exposure duration, 50% duty cycle, and 20% microbubble concentration, pAcGFP1-C1-Ngb-transfected cells exhibited the highest cell viability and transfection efficiency. The efficiency of plasmid delivery was significantly higher with UTMD than transfection with plasmid alone, transfection with plasmid using microbubbles, or transfection of plasmid by ultrasound. In addition, during cobalt chloride-induced hypoxia, caspase-3 activity in pAcGFP1-C1-Ngb-transfected cells was significantly lower than in untransfected cells. Ngb protein and mRNA expression were significantly higher in cells transfected by UTMD than in cells transfected with the other methods. These results demonstrate that UTMD can very efficiently mediate exogenous gene delivery, and that Ngb overexpression protects cells against cobalt chloride-induced hypoxia. | Qian Yang Dianwen Gao Qingzhu Nie Zhengang Cai Jian Du Lujuan Shan Yuejian Liu | 2011 | Neural Regeneration Research2011，6，25： | 3 |
| 6 | Excessive plant compensatory growth:a potential endogenous driver of meadow degradation on the Qinghai-Tibetan Plateau显示文摘Degradation of meadow ecosystems in the largest alpine region of the world,i.e.,the Qinghai-Tibetan Plateau(QTP),is a crucial ecological issue that has ardently discussed in recent years.Many factors,such as livestock overgrazing,climate change and overpopulation of small mammals are treated as important factors that cause the degradation of meadow ecosystems in the QTP.However,there are few hypotheses focus on the potential role of plant compensatory growth on meadow degradation.We proposed a compensatory growth-related hypothesis to understand the potential degradation process of meadow ecosystems in the QTP.We discussed that there are two stages of meadow degradation,i.e.the beginning stage of meadow degradation that is triggered by high-strength overcompensation;and the intensification stage of meadow degradation,which are driven by external factors such as climate warming,small mammals and thawing of permafrost.The mechanism of meadow degradation driven by plant compensatory growth is the asynchronism of plant consumption and the availability of soil nutrients.Our hypothesis that plant compensatory growth drives meadow degradation under the overgrazing condition requires re-examination and modification by testing the balance between soil nutrient cycling rates and the strength of plant compensatory growth in alpine regions. | Yong Zhang Hasbagan Ganjurjav Shikui Dong Qingzhu Gao | 2020 | Ecosystem Health and Sustainability2020，6，1： | 1 |
| 7 | Alpine grassland degradation index and its response to recent climate variability in Northern Tibet, Chin显示文摘 | Gao Qingzhu Wan Yunfan Xu Hongmei | 2010 | Quaternary Interna- tional2010，226，12： | 1 |
| 8 | Mechanism and rate constants for complete series reactions of 19 fluorophenols with atomic H显示文摘Fluorine-containing halogenated fluorophenol may have efect as intermediate species involved in the formation of polyfluorinated dibenzo-p-dioxin/dibenzofurans(PFDDs/Fs). The mechanism for the atomic H initiated reactions with complete series of nineteen fluorophenol congeners was studies using the density functional theory. At the MPWB1K/6-31+G(d,p) level, the geometries and frequencies of reactants, transition states, and products were obtained, and the accurate energetic values were acquired at the MPWB1K/6-311+G(3df,2p) level. The rate constants were evaluated by the canonical variational transition-state theory with the small curvature tunneling contribution over a wide temperature range of 600–1000 K. The study shows that the intramolecular hydrogen-bond in the ortho-substituted FPs as well as the inductive efect of the electron-withdrawing fluorine and steric repulsion of multiple substitutions may ultimately be responsible for the relative strength of the O–H bonds in FPs. The results can be used for further studies on PFDD/Fs formation mechanism. | Rui Gao Xiaoyan Sun Wanni Yu Qingzhu Zhang Wenxing Wang | 2014 | Journal of Environmental Sciences2014，26，1： | 1 |
| 9 | Size-resolved aerosol ionic composition and secondary formation at Mount Heng in South Central China显示文摘理解解决尺寸的喷雾器在南方华中在上面的边界层影响第二等的喷雾器形成的离子的作文和因素,分离尺寸的喷雾器样品在在山 Heng ( 1269 m asl )的顶点的春天 2009 用微孔的一致存款 impactor ( MOUDI )被收集,由 13 个无机、器官的水溶性的离子的随后的实验室分析列在后面。在 non-dust-storm 时期期间,普通下午 < 潜水艇 class= “ a-plus-plus ” > 1.8 集中是 41.8 g?? | Xinfeng WANG Wenxing WANG Likun XUE Xiaomei GAO Wei NIE Yangchun YU Yang ZHOU Lingxiao YANG Qingzhu ZHANG Tao WANG | 2013 | Frontiers of Environmental Science & Engineering2013，7，6： | 1 |
| 10 | Formation of secondary organic carbon and long-range transport of carbonaceous aerosols at Mount Heng in South China显示文摘 | Shengzhen Zhou Zhe Wang Rui Gao Likun Xue Chao Yuan Tao Wang Xiaomei Gao Xinfeng Wang Wei Nie Zheng Xu Qingzhu Zhang Wenxing Wang | 2012 | Atmospheric Environment2012，，： | 1 |
| 11 | Formation of secondary organic carbon and cloud impact on carbonaceous aerosols at Mount Tai, North China显示文摘 | Zhe Wang Tao Wang Jia Guo Rui Gao Likun Xue Jiamin Zhang Yang Zhou Xuehua Zhou Qingzhu Zhang Wenxing Wang | 2011 | Atmospheric Environment2011，，： | 1 |
| 12 | O-GlcNAcylation of YTHDF2 promotes HBV-related hepatocellular carcinoma progression in an N^(6)-methyladenosine-dependent manner显示文摘Hepatitis B virus(HBV)infection is a major risk factor for hepatocellular carcinoma(HCC),but its pathogenic mechanism remains to be explored.The RNA N^(6)-methyladenosine(m^(6)A)reader,YTH(YT521-B homology)domain 2(YTHDF2),plays a critical role in the HCC progression.However,the function and regulatory mechanisms of YTHDF2 in HBV-related HCC remain largely elusive.Here,we discovered that YTHDF2 O-GlcNAcylation was markedly increased upon HBV infection.O-GlcNAc transferase(OGT)-mediated O-GlcNAcylation of YTHDF2 on serine 263 enhanced its protein stability and oncogenic activity by inhibiting its ubiquitination.Mechanistically,YTHDF2 stabilized minichromosome maintenance protein 2(MCM2)and MCM5 transcripts in an m^(6)A-dependent manner,thus promoting cell cycle progression and HBV-related HCC tumorigenesis.Moreover,targeting YTHDF2 O-GlcNAcylation by the OGT inhibitor OSMI-1 significantly suppressed HCC progression.Taken together,our findings reveal a new regulatory mechanism for YTHDF2 and highlight an essential role of YTHDF2 O-GlcNAcylation in RNA m^(6)A methylation and HCC progression.Further description of the molecular pathway has the potential to yield therapeutic targets for suppression of HCC progression due to HBV infection. | Yang Yang Yu Yan Jiaxin Yin Ni Tang Kai Wang Luyi Huang Jie Hu Zhongqi Feng Qingzhu Gao Ailong Huang | 2023 | Signal Transduction and Targeted Therapy2023，8，3： | 0 |
| 13 | Mechanism and kinetic properties of NO_3-initiated atmospheric degradation of DDT显示文摘In this article, the NO3 radical-initiated atmospheric oxidation degradation of DDT was theoretically investigated using molecular orbital theory calculations. All the calculations of intermediates, transition states and products were performed at the MPWB1K/6-311+G(3df,2p)//MPWB1K/6-31+G(d,p) level of theory. Several energetically favorable reaction pathways were revealed. The formation mechanisms of secondary pollutants were presented and discussed. The rate constants were deduced over the temperature range of 273–333 K using canonical variational transition-state(CVT) theory with the small curvature tunneling(SCT) method. Our study shows that H abstraction from the alkyl group and NO3 addition to the C3 atom of the benzene ring are the dominant reaction pathways. The rate-temperature formula of the overall rate constants is k(T)(DDT+NO3) =(7.21 × 10.15)exp(–153.81/T) cm3/(mol·sec) over the possible atmospheric temperature range of 273–333 K. The atmospheric lifetime of DDT determined by NO3 radical is about 52.5 days, which indicates that it can be degraded in the gas phase within several months. | Cai Liu Shanqing Li Rui Gao Juan Dang Wenxing Wang Qingzhu Zhang | 2014 | Journal of Environmental Sciences2014，26，3： | 0 |
| 14 | Analysis of LULUCF Accounting Rules after 2012显示文摘陆地使用,陆地使用变化和林地(LULUCF ) 活动能允许附属建筑我在京都协议狂欢减少他们的碳排放减小压力,并且可比较地为他们的国内工业和精力生产扩展更多的排放空间。枪眼源于 LULUCF 活动类型,特定的财务方法总是在不同聚会之中被说服,特别地包括收获木头产品,力量无可抗拒的影响,引用水平的阀值值,并且粗野网或网网财务方法。为在财务枪眼估计无常,并且到避免开发那国家利用 LULUCF 的财务枪眼减少他们的排放减小压力, LULUCF 数据在附属建筑从主要发达国家认为包括 EU 27,加拿大,日本,和俄国,我是镇定的。根据这些数据的分析,枪眼影响 LULUCF 的财务结果。结果证明收获木头产品的无常是过多的。没有力量无可抗拒, LULUCF 活动生产的碳水池将平均增加至少 30% 。碳水池的参考水平的阀值价值应该被设为高水平。网网财务方法可能对在 2012 以后的 LULUCF 更合适。引证刘, S. ,李·伊, Q. 高,等, 2011:在 2012 以后的 LULUCF 财务规则的分析。副词。Clim。变化物件, 2 (4 ) , doi:10.3724/SP .J.1248.2011.00178。 | Shuo Liu Yu'e Li Qingzhu Gao Yunfan Wan Xin Ma Xiaobo Qin | 2011 | Advances in Climate Change Research2011，2，4： | 0 |
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