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| 1 | Effect of Pulsed Magnetic Field on the Residual Stress of Rolled Magnium Alloy AZ31 Sheet显示文摘A novel method of pulsed magnetic field(PMF)treatment was developed to eliminate the residual stress of rolled magnesium alloy AZ31 sheet in this study.The eff ect of PMF on residual stress of rolled AZ31 sheet was investigated and its mechanism was analyzed.The experimental results revealed that the pulse frequency had a significant impact on residual stress.After 10.0 Hz PMF treatment,the average and maximum reduction rates of residual stress along the rolled direction were 26.6%and 30.3%,respectively.It was found that the dislocation density and parallel dislocation in grains of the rolled sheet increased after it was treated by the pulsed magnetic field.The simulation results showed that the Lorentz force generated by the pulsed magnetic field can lead to basal slip,thereby resulting in local plastic deformation.Besides,the Joule heat produced during the PMF treatment was conducive to the elimination of residual stress. | Meng Yan Cong Wang Tianjiao Luo Yingju Li Xiaohui Feng Qiuyan Huang Yuansheng Yang | 2021 | Acta Metallurgica Sinica(English Letters)2021，34，1： | 3 |
| 2 | Generation and characterization of stable pig pregastrulation epiblast stem cell lines显示文摘Pig epiblast-derived pluripotent stem cells are considered to have great potential and broad prospects for human therapeutic model developme nt and livestock breeding.Despite on going attempts since the 1990s,no stably defined pig epiblast-derived stem cell line has bee n established.Here,guided by in sights from a large-scale sin gle-cell tran scriptome an alysis of pig embryos from embryonic day(E)0 to E14,specifically,the tracing of pluripotency changes during epiblast development,we developed an in vitro culture medium for establishing and maintaining stable pluripotent stem cell lines from pig E10 pregastrulation epiblasts(pgEpiSCs).Enabled by chemical inhibition of WNT-related signaling in combination with growth factors in the FGF/ERK,JAK/STAT3,and Activin/Nodal pathways,pgEpiSCs maintain their pluripotency transcriptome features,similar to those of E10 epiblast cells,and normal karyotypes after more than 240 passages and have the potential to differentiate into three germ layers.Strikingly,ultradeep in situ Hi-C analysis revealed functional impacts of chromatin 3D-spatial associations on the transcriptional regulation of pluripote ncy marker genes in pgEpiSCs.I n practice,we con firmed that pgEpiSCs readily tolerate at least three rounds of successive gene editi ng and gene rated cloned gen e-edited live piglets.Our findings deliver on the long-a nticipated promise of pig pluripote nt stem cells and open new avenues for biological research,animal husbandry,and regenerative biomedicine. | Minglei Zhi Jinying Zhang Qianzi Tang Dawei Yu Shuai Gao Dengfeng Gao Pengliang Liu Jianxiong Guo Tang Hai Jie Gao Suying Cao Zimo Zhao Chongyang Li Xiaogang Weng Mengnan He Tianzhi Chen Yingjie Wang Keren Long Deling Jiao Guanglei Li Jiaman Zhang Yan Liu Yu Lin Daxin Pang Qianqian Zhu Naixin Chen Jingjing Huang Xinze Chen Yixuan Yao Jingcang Yang Zicong Xie Xianya Huang Mengxin Liu Ran Zhang Qiuyan Li Yiliang Miao Jianhui Tian Xingxu Huang Hongsheng Ouyang Bofeng Liu Wei Xie Qi Zhou Zhonghua Liu Caihong Zheng Mingzhou Li Jianyong Han | 2022 | Cell Research2022，32，4： | 3 |
| 3 | Identification of a suitable endogenous control gene in porcine blastocysts for use in quantitative PCR analysis of microRNAs显示文摘To obtain reliable results in quantitative PCR (qPCR) reactions, an endogenous control (EC) gene is needed to correct for systematic variations. In this study, a TaqMan low density array was used to quantify the expression levels of microRNA (miRNA) genes in in vivo fertilized, in vitro fertilized, parthenogenetic and somatic cell nuclear transfer blastocysts. The aim was to identify suitable EC genes for the qPCR analysis of miRNAs in porcine blastocysts. The results showed that thirty-six miRNAs were commonly expressed in the four kinds of embryos and the expression levels of eleven miRNAs were similar in the different embryo types (P-value>0.05). These 11 miRNAs were selected as candidate EC genes for further analysis and, of these, miR-16 was identified as the most stable EC gene by the GeNorm (a tool based on a pair-wise comparison model that calculates the internal control genes stability measure and determines the most reliable pair of EC genes) and NormFinder (an excel plug-in that uses an ANOVA-based model to estimate intra-and inter-group variation to indicate the single most stable EC gene) programs. In addition, a cell number normalization method validated miR-16 as a suitable EC gene for use in future qPCR analysis of miRNAs in porcine blastocysts. | LI Jun WEI HengXi LI Yan LI QiuYan LI Ning | 2012 | Science China(Life Sciences)2012，55，2： | 2 |
| 4 | Production of human lysozyme-transgenic cloned porcine embryos by somatic nuclear transfer显示文摘Due to their physiology and organ size, pigs have significant potential as human disease models and as organ transplantation donors. Genetic modification of pigs could provide benefits for both agriculture and human medicine. In this study, five fetal pig fibroblast cell lines from two species (Wuzhishan and Landrace pigs) were transfected using double-marked human lysozyme (HLY) plasmids (pBC1-HLY-GFP-NEO) by a liposome-mediated method. The ratio of green fluorescent protein (GFP)-expressing cells was >95% in sw7, sw8, slw3 and slw6 cell lines, but only 49.3% in slw9 cells. Cells from the four highly transgenic lines were used as nuclear donors to construct embryos, which were then cultured after fusion and activation by electric stimulation. The rate of cleavage was 76.7%, 48 h after activation. After 7 days, 18.5% of cleaved eggs had developed to the blastocyst stage and 93.3% of blastocysts were GFP-positive. These results indicate that transgenic fetal pig flbroblast cell lines could be obtained by a liposome-mediated method, though the transfection efficiency varied between cell lines. Reconstructed embryos derived from transgenic cells could successfully develop into blastocysts, most of which were GFP-positive. | Qiuyan Li Hengxi Wei Ying Guo Yan Li Rui Zhao Yufang Ma Zhengquan Yu Bo Tang Lei Zhang Yunping Dai Ning Li | 2009 | Progress in Natural Science:Materials International2009，19，6： | 2 |
| 5 | Electrostatic assembly functionalization of poly(γ-glutamic acid)for biomedical antibacterial applications显示文摘Poly(γ-glutamic acid)(γ-PGA)has been found widespread applications in biomedical field because of its excellent water solubility,biocompatibility,and bioactivity.Herein,a water-insoluble γ-PGA antibacterial compound is facilely fabricated via one-pot electrostatic assembly of γ-PGA with cationic ethyl lauroyl arginate(ELA).The functionalized γ-PGA compound(γ-PGA-ELA)ethanol solution can facilely produce colorless and transparent coatings on various inorganic,metal,and polymeric substrates,especially for the lumen of slender catheters(length up to 2 m,and inner diameter down to 1 mm).The functionalized γ-PGA coating presents remarkable antibacterial efficacy in vitro and in vivo.In addition,the γ-PGA compound is used as antibacterial additives of polyolefin via melting extrusion,and the asprepared antibacterial polyolefin demonstrates advantageous antibacterial efficacy.More importantly,the functionalized γ-PGA coating exhibit good hemocompatibility,low cytotoxicity,and satisfactory histocompatibility.The as-proposed γ-PGA compound has a great potential to serve as a safe and multifunctional antibacterial candidate to combat biomedical devices-related infections. | Xiaodan Wang Hengchong Shi Haoyu Tang Huan Yu Qiuyan Yan Huawei Yang Xu Zhang Shifang Luan | 2020 | Journal of Materials Science & Technology2020，59，24： | 1 |
| 6 | Lercanidipine hydrochloride versus felodipine sustained-release for mild-to-moderate hypertension: a multi-center, randomized clinical trial显示文摘 | Ying Wu Mengdan Xu Hao Wang Xin Xu Shuiping Zhao Mei Zhang Huigen Jin Jinchuan Yan Bangning Wang Jianbin Gong Xiang Lu Jianqiang Peng Qiuyan Dai | 2014 | Current Medical Research & Opinion2014，，1： | 1 |
| 7 | Prenatal ultrasonographic characteristics and prognosis of isolated redundant foramen ovale flap显示文摘Objective:To analyze the prenatal ultrasonographic characteristics and prognosis of the isolated redundant foramen ovale flap(RFOF).Methods:From January 2014 to December 2021,we collected data on fetal echocardiography analyses and perinatal outcomes for fetuses with isolated RFOF in Peking University People's Hospital.Results:We found that 0.31%(87/28308)of participants have RFOF.The four-chamber results of the foramen ovale flap(FOF)showed that it was stiff and extended>50%or reached the lateral wall of the left atrium(LA)in diastole.As seen from the foramen ovale(FO)channel and four-chamber views,the hypermobile and redundant flap were observed shrinking and stretching with the fetal cardiac cycle,which is similar to jellyfish.The lateral displacement of flow from LA to the left ventricle(LV)around the FOF on color doppler demonstrated thin linear blood flow from the right to left and a reversal of flow across FO.A uniphasic,but not biphasic,pattern of FOF displacement was observed on M-mode.Stages I(23/87)and II(51/87)had a higher ratio of ventricular disproportion than Stage 0(11/87)and III(2/87).We observed the RA/LA(right/left atrium)>1.2 in 53 cases(60.9%),RV/LV(right/left ventricle)>1.2 in 53 cases(60.9%),PA/AO(pulmonary/aortic artery)>1.2 in 53 cases(60.9%),and moderate or severe tricuspid regurgitation in 10 cases and moderate pericardial effusion in 2 cases(2.2%).Seventy-four RFOF cases had follow-up data.Neonatal death occurred in 2 cases;72 fetuses survived with normal or minor heart defects.Conclusion:RFOF should be considered if the left side of the heart of a fetus is smaller and related to hypermobile FOF.For isolated RFOF cases,a monthly follow-up is recommended to monitor arrhythmia or fetal hydrop status.Prompt treatment is recommended for those with adequate gestational age and lung maturity. | Yuntao Li Qiuyan Pei Zhenjuan Yang Yani Yan Xiaowei Xue | 2022 | Gynecology and Obstetrics Clinical Medicine2022，2，3： | 1 |
| 8 | Clostridium beijerinckii mutant with high inhibitor tolerance obtained by low-energy ion implantation显示文摘 | Guo Ting Tang Yan Zhang Qiuyan | 2012 | J Ind Microbiol Biotechnol2012，39，： | 1 |
| 9 | Effect of leptin on oocyte maturation and subsequent pregnancy rate of cloned embryos reconstructed by somatic cell nuclear transfer in pigs显示文摘由原子转移(SCNT ) 有的体的房间克隆猪在基本研究,人的药和农业生产的宽应用。到改善克隆效率,二基本成熟媒介的效果, NCSU-23 和 TCM199,被比较,并且 TCM199 为有 leptin 的下列实验被选择。我们系统地在 vitro 成熟(IVM ) 在卵母细胞上学习了 leptin 补充的效果,在激活的单性生殖的联盟者(PA ) 和 SCNT 胚胎的 vitro 开发并且在在胚胎转移(et ) 以后的 SCNT 胚胎的 vivo 开发。结果证明进成熟媒介的 100 或 200 ng/ml leptin 的补充极大地没影响 PA 和 SCNT 的卵母细胞,或劈开率的原子成熟(P >
0.05 ) 。当 100 或 200 ng/ml leptin 在 PA 胚囊被加到房间的成熟媒介,和数字时, PA 和 SCNT 胚胎的胚囊率显著地被改进也被改进(P <
0.05 ) 。在 SCNT 的胚囊的房间的数字被改进,当 100 ng/ml leptin 被增加时(P <
0.05 ) 。而且,到 IVM 媒介的 100 或 200 ng/ml leptin 的补充可以在猪克隆改进怀孕率和交货率。 | Hengxi Wei Qiuyan Li Jun Li Yan Li Yunping Dai Yufang Ma Kai Xue Ning Li | 2008 | Progress in Natural Science:Materials International2008，18，12： | 1 |
| 10 | Identifying Factors of Integrated Industry Based on the Complex Systems Theory显示文摘 | Qiuyan Zhang GuohongWang Ruyu Yan Jing Zhang | 2009 | Journal of Systems Science and Information2009，7，2： | 0 |
| 11 | Study on Improvement of Soil Porosity显示文摘In order to further improve soil porosity and improve soil physical properties,the raw materials,formula and application methods of soil amendment were optimized,and the pH and porosity of yellow soil were compared and analyzed after application of soil amendment.The results showed that the application of amendments increased soil pH and porosity.The results will provide the scientific basis for the improvement of soil porosity. | Xiong YAN Wenzhao LI Xuhui WANG Qiuyan WANG Ling YANG Jun ZHAO | 2022 | Asian Agricultural Research2022，14，4： | 0 |
| 12 | Establishment of a prognostic scoring model for regional recurrent nasopharyngeal carcinoma after neck dissection显示文摘Objective:The main aim of this study was to establish a scoring model to predict risk of progression and survival in patients with regionally recurrent nasopharyngeal carcinoma(NPC).Methods:Three hundred and forty-eight patients subjected to neck dissection from 2003 to 2017 were included for study.Clinicopathologic information for each patient was analyzed.Independent prognostic factors were selected using the Cox proportional hazards model and incorporated into the scoring model.Concordance index(C-index)and calibration curves were used to verify discrimination and calibration,respectively and the results validated using bootstrap resampling.Results:Microscopic positive lymph node>2[hazard ratio(HR),2.19;95%confidence interval(CI),1.30–3.68;P=0.003],extranodal extension(HR,2.75;95%CI,1.69–4.47;P<0.001),and lower neck involvement(HR,1.78;95%CI,1.04–3.04;P=0.034)were identified from multivariate analysis as independent factors for overall survival(OS).A qualitative 4-point scale was generated to stratify patients into 4 risk groups for predicting OS and progression-free survival(PFS).The novel scoring model demonstrated enhanced discrimination(C-index=0.69;95%CI,0.62–0.76)relative to the original recurrent tumor-node-metastasis(rTNM)staging system(C-index=0.56;95%CI,0.50–0.62),and was internally validated with a bootstrap-adjusted C-index of 0.70.The calibration curve showed good agreement between predicted probabilities and actual observations.Conclusions:The scoring system established in this study based on a large regionally recurrent NPC cohort fills a gap regarding assessment of risk and prediction of survival outcomes after neck dissection in this population and could be further applied to identify high-risk patients who may benefit from more aggressive intervention. | Xiaoyun Li Chao Lin Jinjie Yan Qiuyan Chen Xuesong Sun Sailan Liu Shanshan Guo Liting Liu Haojun Xie Qingnan Tang Yujing Liang Ling Guo Hao Li Xuekui Liu Xiang Guo Linquan Tang Haiqiang Mai | 2020 | Cancer Biology & Medicine2020，17，1： | 0 |
| 13 | Thermal annealing promoted room temperature phosphorescence:motion models and internal mechanism显示文摘Thermal annealing has been proven to be an efficient method to optimize the device performance of organic and polymeric opto-electronic materials.However,no detailed information of aggregate structures was obtained for a deeper understanding of what happens during thermal annealing.Herein,through modulation of molecular configurations by tunable linkage positions,and the amplified amplitudes of molecular motions by incorporation of additional methylene units,accurate changes of aggregated structures upon thermal annealing have been achieved,accompanying with the‘turn-on’room temperature phosphorescence(RTP)response by about 4800-and 177-fold increase of lifetimes.The stretching and swing motion models have been proposed,which afforded an efficient way to investigate the science of dynamic aggregation in depth. | Yan Gao Jie Lu Qiuyan Liao Shuhui Li Qianqian Li Zhen Li | 2023 | National Science Review2023，10，11： | 0 |
| 14 | Subsoil tillage enhances wheat productivity,soil organic carbon and available nutrient status in dryland fields显示文摘Tillage practices during the fallow period benefit water storage and yield in dryland wheat crops.However,there is currently no clarity on the responses of soil organic carbon(SOC),total nitrogen(TN),and available nutrients to tillage practices within the growing season.This study evaluated the effects of three tillage practices(NT,no tillage;SS,subsoil tillage;DT,deep tillage)over five years on soil physicochemical properties.Soil samples at harvest stage from the fifth year were analyzed to determine the soil aggregate and aggregate-associated C and N fractions.The results indicated that SS and DT improved grain yield,straw biomass and straw carbon return of wheat compared with NT.In contrast to DT and NT,SS favored SOC and TN concentrations and stocks by increasing the soil organic carbon sequestration rate(SOCSR)and soil nitrogen sequestration rate(TNSR)in the 0-40 cm layer.Higher SOC levels under SS and NT were associated with greater aggregate-associated C fractions,while TN was positively associated with soluble organic nitrogen(SON).Compared with DT,the NT and SS treatments improved soil available nutrients in the 0-20 cm layer.These findings suggest that SS is an excellent practice for increasing soil carbon,nitrogen and nutrient availability in dryland wheat fields in North China. | Qiuyan Yan Linjia Wu Fei Dong Shuangdui Yan Feng Li Yaqin Jia Jiancheng Zhang Ruifu Zhang Xiao Huang | 2024 | Journal of Integrative Agriculture2024，23，1： | 0 |
| 15 | Bibliometric Analysis of Soil Fertility Evaluation Papers Based on Core Journals in CNKI显示文摘[Objectives]This study was conducted to deeply analyze the research status in the field of soil fertility evaluation.[Methods]With the core journals of Peking University included in CNKI database from 2002 to 2021 as the research object,papers were searched in the advanced search mode using SU=‘soil fertility’and SU=‘evaluation’as the retrieval subject terms and analyzed by the bibliometric research method,from three perspectives,namely,the annual number of papers published,the number of papers issued by authors and the number of highly cited papers.[Results]A total of 891 first authors issued 990 papers related to soil fertility evaluation in total from 2002 to 2021,with an average annual number of papers published at about 50,and the number of published papers showed a trend of rapidly increasing first and then gradually stabilizing.The top 13 authors published 3-4 papers,including Yang Xiaojuan,Chen Haisheng and Li Xinju(4 papers)ranking first.The top 10 papers with the highest citation frequency were published in 9 journals,respectively covering ecology,forestry,agriculture,geography,engineering and other fields,which shows that there are many kinds of journals focusing on the research field of soil fertility evaluation.[Conclusions]The results can provide reference for further research on soil fertility evaluation. | Huaidan ZHANG Xiong YAN Wenzhao LI Jinghua WEN Yuanyuan LI Xuhui WANG Qiuyan WANG | 2022 | Agricultural Biotechnology2022，11，6： | 0 |
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