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| 1 | A novel m6A reader Prrc2a controls oligodendroglial specification and myelination显示文摘While N6-methyladenosine (m6A), the most abundant internal modification in eukaryotic mRNA, is linked to cell differentiation and tissue development, the biological significance of m6A modification in mammalian glial development remains unknown. Here, we identify a novel m6A reader, Prrc2a (Proline rich coiled-coil 2A), which controls oligodendrocyte specification and myelination. Nestin-Cre-mediated knockout of Prrc2a induces significant hypomyelination, decreased lifespan, as well as locomotive and cognitive defects in a mouse model. Further analyses reveal that Prrc2a is involved in oligodendrocyte progenitor cells (OPCs) proliferation and oligodendrocyte fate determination. Accordingly, oligodendroglial-lineage specific deletion of Prrc2a causes a similar phenotype of Nestin-Cre-mediated deletion. Combining transcriptome-wide RNA-seq, m6A-RIP-seq and Prrc2a RIP-seq analysis, we find that Olig2 is a critical downstream target gene of Prrc2a in oligodendrocyte development. Furthermore, Prrc2a stabilizes Olig2 mRNA through binding to a consensus GGACU motif in the Olig2 CDS (coding sequence) in an m6A-dependent manner. Interestingly, we also find that the m6A demethylase, Fto, erases the m6A modification of Olig2 mRNA and promotes its degradation. Together, our results indicate that Prrc2a plays an important role in oligodendrocyte specification through functioning as a novel m6A reader. These findings suggest a new avenue for the development of therapeutic strategies for hypomyelination-related neurological diseases. | Rong Wu Ang Li Baofa Sun Jian-Guang Sun Jinhua Zhang Ting Zhang Yusheng Chen Yujie Xiao Yuhao Gao Qingyang Zhang Jun Ma Xin Yang Yajin Liao Wei-Yi Lai Xiaolong Qi Shukun Wang Yousheng Shu Hai-Lin Wang Fengchao Wang Yun-Gui Yang Zengqiang Yuan | 2019 | Cell Research2019，29，1： | 32 |
| 2 | Augmented reality technology for preoperative planning and intraoperative navigation during hepatobiliary surgery: A review of current methods显示文摘Background: Augmented reality(AR) technology is used to reconstruct three-dimensional(3D) images of hepatic and biliary structures from computed tomography and magnetic resonance imaging data, and to superimpose the virtual images onto a view of the surgical field. In liver surgery, these superimposed virtual images help the surgeon to visualize intrahepatic structures and therefore, to operate precisely and to improve clinical outcomes.Data Sources: The keywords 'augmented reality', 'liver', 'laparoscopic' and 'hepatectomy' were used for searching publications in the Pub Med database. The primary source of literatures was from peer-reviewed journals up to December 2016. Additional articles were identified by manual search of references found in the key articles.Results: In general, AR technology mainly includes 3D reconstruction, display, registration as well as tracking techniques and has recently been adopted gradually for liver surgeries including laparoscopy and laparotomy with video-based AR assisted laparoscopic resection as the main technical application. By applying AR technology, blood vessels and tumor structures in the liver can be displayed during surgery,which permits precise navigation during complex surgical procedures. Liver transformation and registration errors during surgery were the main factors that limit the application of AR technology.Conclusions: With recent advances, AR technologies have the potential to improve hepatobiliary surgical procedures. However, additional clinical studies will be required to evaluate AR as a tool for reducing postoperative morbidity and mortality and for the improvement of long-term clinical outcomes. Future research is needed in the fusion of multiple imaging modalities, improving biomechanical liver modeling,and enhancing image data processing and tracking technologies to increase the accuracy of current AR methods. | Rui Tang Long-Fei Ma Zhi-Xia Rong Mo-Dan Li Jian-Ping Zeng Xue-Dong Wang Hong-En Liao Jia-Hong Dong | 2018 | Hepatobiliary & Pancreatic Diseases International2018，17，2： | 27 |
| 3 | Expenditure and financial burden for the diagnosis and treatment of colorectal cancer in China:a hospital.based,multicenter,cross-sectional survey显示文摘Background: The increasing prevalence of colorectal cancer(CRC) in China and the paucity of information about relevant expenditure highlight the necessity of better understanding the financial burden and effect of CRC diagnosis and treatment. We performed a survey to quantify the direct medical and non-medical expenditure as well as the resulting financial burden of CRC patients in China.Methods: We conducted a multicenter, cross-sectional survey in 37 tertiary hospitals in 13 provinces across China between 2012 and 2014. Each enrolled patient was interviewed using a structured questionnaire. All expenditure data were inflated to the 2014 Chinese Yuan(CNY; 1 CNY = 0.163 USD). We quantified the overall expenditure and financial burden and by subgroup(hospital type, age at diagnosis, sex, education, occupation, insurance type, household income, clinical stage, pathologic type, and therapeutic regimen). We then performed generalized linear modeling to determine the factors associated with overall expenditure.Results: A total of 2356 patients with a mean age of 57.4 years were included, 57.1 % of whom were men; 13.9% of patients had stage I cancer; and the average previous-year household income was 54,525 CNY.The overall average direct expenditure per patient was estimated to be 67,408 CNY, and the expenditures for stage Ⅰ, Ⅱ, Ⅲ, and Ⅳ disease were 56,099 CNY, 59,952 CNY, 67,292 CNY, and 82,729 CNY, respectively. Non-medical expenditure accounted for 8.3%of the overall expenditure. The 1-year out-of-pocket expenditure of a newly diagnosed patient was 32,649 CNY, which accounted for 59.9% of their previous-year household income and caused 75.0% of families to suffer an unmanageable financial burden. Univariate analysis showed that financial burden and overall expenditure differed in almost all subgroups(P < 0.05), except for sex. Multivariate analysis showed that patients who were treated in specialized hospitals and those who were diagnosed with adenocarcinoma or diagnosed at a later stage were likely to spend more,whereas those with a lower household income and those who underwent surgery spent less(all P < 0.05).Conclusions: For patients in China, direct expenditure for the diagnosis and treatment of CRC seemed catastrophic,and non-medical expenditure was non-ignorable. The financial burden varied among subgroups, especially among patients with different clinical stages of disease, which suggests that, in China, CRC screening might be cost-effective. | Hui-Yao Huang Ju-Fang Shi Lan-Wei Guo Ya-Na Bai Xian-Zhen Liao Guo-iangLiu A-Yan Mao Jian-Song Ren Xiao-Jie Sun Xin-Yu Zhu Le Wang Bing-Bing Song Ling-Bin Du Lin Zhu Ji-Yong Gong Qi Zhou Yu-Qin Liu Rong Cao Ling Mai Li Lan Xiao-Hua Sun Ying Ren Jin-Yi Zhou Yuan-Zheng Wang Xiao Qi Pei-An Lou Dian Shi Ni Li Kai Zhang Jie He Min Dai | 2017 | Chinese Journal of Cancer2017，36，8： | 25 |
| 4 | Persistence of hepatitis B vaccine immune protection and response to hepatitis B booster immunization显示文摘AbstractAIMToidentifythepersistenceofimmuneprotectionofChinamade,plasmaderivedhepatitisBvaccineafterinfancyimmunizationandt... | LI Hui 1, LI Rong Cheng 2, LIAO Su Su 1, YANG Jin Ye 2, ZENG Xian Jia 1 and WANG Shu Sheng 2 | 1998 | World Journal of Gastroenterology1998，4，6： | 15 |
| 5 | Long-term efectiveness of infancy low-dose hepatitis B vaccine immunization in Zhuang minority area in China显示文摘METHODSTwoepidemiologicmethods,acrosssectionalfolowupstudyandacasecontrolstudy,wereusedfortheevaluationoftheserologicalef... | LI Hui 1, LI Rong Cheng 2, LIAO Su Su 1, GONG Jian 2, ZENG Xian Jia 1 and LI Yan Ping 2 | 1999 | World Journal of Gastroenterology1999，5，2： | 14 |
| 6 | Study on the anticarcinogenic effect and acutetoxicity of liver-targeting mitoxantrone-nanoparticles显示文摘AIM To study the anticarcinogenic effect and acute toxicity of liver targeting mitoxantrone nanospheres. METHODS The anticarcinogenic effect of mitoxantrone polybutylcyanoacrylate nanoparticles (DHAQ PBCA NP) was investigated by using heterotopic and orthotopic transplantation models of human hepatocellular carcinoma (HCC) in nude mice and was compared with mitoxantrone (DHAQ) and doxorubicin (ADR). The acute toxicity of DHAQ PBCA NP lyophilized injection in mice was also studied. RESULTS The tumor inhibition rates of ADR, DHAQ, DHAQ PBCA NP to orthotopically transplanted HCC were 60 07%, 67 49% and 99 44%, respectively, but regard to heterotopically transplanted HCC, these were 80 03%, 86 18% and 92 90%, which were concordant with the results acquired by mitosis counting and proliferating cell nuclear antigen (PCNA). After iv administration to mice with DHAQ PBCA NP, the LD 50 was 16 9*!mg/*!kg ± 3 9*!mg/*!kg , no obvious local irritation was observed and there was no significant damage to the structure of liver cells, and that of the heart, spleen and kidneys. CONCLUSION The effect of DHAQ PBCA NP was significantly higher than that of DHAQ and ADR in the anti orthotopically transplanted HCC and the acute toxicity was relatively low. | ZHANG Zhi Rong, HE Qin, LIAO Gong Tie and BAI Shao Huai | 1999 | World Journal of Gastroenterology1999，5，6： | 13 |
| 7 | Effects of transplanted myoblasts transfected with human growth hormone gene on improvement of ventricular function of rats显示文摘背景:为心肌的修理的房间移植被早细胞死亡限制。有人的生长激素(hGH ) 的基因治疗被显示了在实验动物支持血管生成和 attenuate apoptosis。这研究被进行在心肌的梗塞以后在心功能恢复和血管生成上探索基于 myoblast 的 hGH 基因治疗的效果,并且比较基于 myoblast 的 hGH 基因治疗和成肌细胞治疗之间的差别。方法:Myoblasts 从几只 SD 老鼠被孤立,有教养,净化,并且有原生质标志 pLghGHSN 和 pLgGFPSN 的 transfected。放射性免疫测定(RIA ) 被用来在这些 myoblasts 检测 hGH 的表示。SD 老鼠经历了左前面的下降冠的动脉的结扎以便建立一个心局部缺血模型。在离开了冠的动脉吸藏以后,经历了结扎的三十幸存老鼠 2 个星期随机被划分成 3 个相等的组:pLghGHSN 组织感染 hGH 基因移植的收到的成肌细胞;pLgGFPSN 组织感染 GFP 基因移植的收到的成肌细胞;控制组织:仅仅收到了有教养的媒介。在注射以后的四个星期幸存老鼠由回响心动描记法经历了心脏的功能的评估。老鼠被打死,室的样品是有 hematoxylin 曙红和第八因子的经历的免疫组织化学。Cryosection 被萤光显微镜检查分析检验绿荧光灯的蛋白质的表示。反向的 transcriptase 聚合酶链反应(RT-PCR ) 被用来检验脉管的内皮生长因素(VEGF ) 的 mRNA 表示, bax 和 Bcl-2。在心肌层的 hGH 表示被西方的污点检验。结果:成肌细胞能成功地被孤立,有教养并且 transfected。在 transfected 成肌细胞的 hGH 的表示与 RIA 被表明。在治疗以后的四个星期,心脏的功能在 pLghGHSN 显著地被改进组和 pLgGFPSN 组。部分弄短(FS ) 并且在 pLghGHSN 组的喷射部分(EF ) 在治疗以后与 pLgGFPSN 组和控制组相比显著地被提高(FS:36.9+/-5.3 对 29.5+/-3.5, 21.8+/-2.9;EF:56.9+/-4.3 对 47.1+/-3.6, 38.4+/-4.8, P<0.05 ) 。左室的结束心脏舒张的尺寸(LVEDD ) 和在 pLghGHSN 组的心 infracted 尺寸在治疗以后与 pLgGFPSN 组和控制组相比显著地被减少(LVEDD:5.9+/-0.3 对 6.8+/-0.2, 8.6+/-0.3;心 infracted 尺寸:(34.5+/-4.2 )% 对(40.0+/-3.9 )% ,(46.1+/-3.8 )% , P<0.05 ) ;格林荧光在 pLgGFPSN 组的 cryosection 被检测。pLgGFPSN 组的毛状的密度比 pLghGHSN 组和控制组(P<0.05 ) 的那些显著地大。在 pLghGHSN 组的 VEGF 和 Bcl-2/bax 的 mRNA 表达式比在 pLgGFPSN 组或控制组(P<0.05 ) 高。在心肌层织物的 hGH 基因的表示能被西方的污点试金在 pLghGHSN 组检测。结论:有 hGH 的心房间 transfected 的移植与 GFP 比房间 transfected 的移植导致了 antiapoptosis 的更大的血管生成和效果。联合 GH 基因转移和房间移植为改进柱子梗塞提供了有效策略室的功能。 | RONG Shu-ling LU Yong-xin LIAO Yu-hua WANG Xiao-lin WANG Yong-jin CHANG Chao WANG Yu-qin LIU Qi-yun GAO Yan-zhang MI Shao-hua | 2008 | Chinese Medical Journal2008，，4： | 12 |
| 8 | Aspartic acid in the hippocampus:a biomarker for postoperative cognitive dysfunction显示文摘This study established an aged rat model of cognitive dysfunction using anesthesia with 2% isoflurane and 80% oxygen for 2 hours. Twenty-four hours later, Y-maze test results showed that isoflurane significantly impaired cognitive function in aged rats. Gas chromatography-mass spectrometry results showed that isoflurane also significantly increased the levels of N,N-diethylacetamide, n-ethylacetamide, aspartic acid, malic acid and arabinonic acid in the hippocampus of isoflurane-treated rats. Moreover, aspartic acid, N,N-diethylacetamide, n-ethylacetamide and malic acid concentration was positively correlated with the degree of cognitive dysfunction in the isoflurane-treated rats. It is evident that hippocampal metabolite changes are involved in the formation of cognitive dysfunction after isoflurane anesthesia. To further verify these results, this study cultured hippocampal neurons in vitro, which were then treated with aspartic acid(100 μmol/L). Results suggested that aspartic acid concentration in the hippocampus may be a biomarker for predicting the occurrence and disease progress of cognitive dysfunction. | Rong Hu Dong Huang Jianbin Tong Qin Liao Zhonghua Hu Wen Ouyang | 2014 | Neural Regeneration Research2014，9，2： | 12 |
| 9 | Effects of recombinant retroviral vector mediated human insulin like growth factor-1 gene transfection on skeletal muscle growth in rat显示文摘Background This study transferred a recombinant gene encoding human insulin like growth factor-1 (hIGF-1) into modified primary skeletal myoblasts with a retroviral vector (pLgXSN ) and determined whether the hIGF-1 promoted growth of skeletal muscle in rat. Methods hIGF-1cDNA was amplified in vitro from normal human liver cells by using RT-PCR and cloned into plasmid vector pLgXSN. The recombinant vector pLghIGF-1SN and control vector pLgGFPSN were transfected into packaging cell PT67 and G418 was used to select positive colony. Myoblasts were infected with a high titre viral supernatant and transduction efficiency was evaluated as GFP expression. The expression of hIGF-1 mRNA in myoblasts was investigated by immunocytochemistry and RT-PCR. MTT assays detected the growth of myoblasts in vitro. Myoblasts transduced with pLghIGF-1SN were injected into hind limb muscles of 10-12 week male SD rats. Formed tissues were harvested 4 weeks later. Myocyte diameter, mean weight of hind limb and body were measured to evaluate the skeletal muscle growth. Results Recombinant retroviral plasmid vector pLghIGF-1SN was constructed successfully. The titre of the packaged recombinant retrovirus was 1×106 cfu/ml. The transfection rate of PT67 cells reached 100% after G418 screening. hIGF-1 expression was positive in myoblast-IGF-1. The proliferation rate of myoblast-IGF-1 in vitro was higher than GFP-myoblast or myoblast (P< 0.05). The mean weights of hind limb and body of rats injected myoblast-IGF-1 were higher than those of the rats injected with myoblast-GFP or myoblast (P< 0.05). Myocyte diameter had a significant increase in IGF-1 group compared to GFP group and myoblast group (P< 0.05). Conclusions The transfection of the human IGF-1 gene mediated by a retroviral vector can promote the growth of skeletal muscle in rats. Genetically modified primary skeletal myoblasts provide a possibly effective approach to treat some skeletal muscle diseases. | RONG Shu-Ling LU Yong-Xin LIAO Yu-Hua WANG Xiao-Lin GUO He-Ping CHANG Chao GAO Yan-Zhang MI Shao-Hua Wan Jian-Ping | 2006 | Chinese Medical Journal2006，，23： | 10 |
| 10 | Clinical Characteristics of Children with Coronavirus Disease 2019 in Hubei, China显示文摘Since December 2019,COVID-19 has occurred unexpectedly and emerged as a health problem worldw ide.Despite the rapidly increasing number of cases in subsequent weeks,the clinical characteristics of pediatric cases are rarely described.A cross-sectional multicenter study was carried out in 10 hospitals across Hubei province.A total of 25 confirmed pediatric cases of COVID-19 were collected.The demographic data,epidemiological history,underlying discascs,clinical mani festations,laboratory and radiological data,treatments,and outcomes were analyzcd.Of 25 hospitalized patients with COVID-19,the boy to girl ratio was 1.27:1.The median age was 3 years.COVID-19 cases in children aged<3 years,3-6 years,and≥6-years patients were 10(40%),6(24%),and 9(36%),respectively.The most common symptoms at onset of ilness were fever(13[52%]),and dry cough(11[44%]).Chest CT images showed essential normal in 8 cases(33.3%),unilateral involvement of lungs in 5 cases(20.8%),and bilateral involvement in 11 cases(45.8%).Clinical diagnoses included upper respiratory tract infection(n=8),mild pneumonia(n=15),and critical cases(n=2).Two critical cases(8%)were given invasive mechanical ventilation,corticosteroids,and immunoglobulin.The symptoms in 24(96%)of 25 patients were alleviated and one patient had been discharged.It was concluded that children were susceptible to COVID-19 like adults,while the clinical presentations and outcomes were more favorable in children.However,children less than 3 years old accounted for majority cases and critical cases lied in this age group,which demanded extra attentions during home caring and hospitalization treatment. | Fang ZHENG Chun LIAO Qi-hong FAN Hong-bo CHEN Xue-gong ZHAO Zhong-guo XIE Xi-lin LI Chun-xi CHEN Xiao-xia LU Zhi-sheng LIU Wei LU Chun-bao CHEN Rong JIAO Ai-ming ZHANG Jin-tang WANG Xi-wei DING Yao-guang ZENG Li-ping CHENG Qing-feng HUANG Jiang WU Xi-chang LUO Zhu-jun WANG Yan-yan ZHONG Yan BAI Xiao-yan WU Run-ming JIN | 2020 | Current Medical Science2020，40，2： | 10 |
| 11 | Tectonic Evolution of the Tianhuan Depression and the Western Margin of the Late Triassic Ordos显示文摘The Ordos Basin is one of the most important oil and gas basins in China. Based on surface outcrop, key exploratory wells and seismic reflection data and by using the technology of 'prototype basin recovery', seismic profile 'layer flattening' and 'restoration of balanced section',and other methods, the sedimentary boundary, structure and the evolution history of the Tianhuan depression on the western margin of the Ordos Basin are reestablished. The following results have been obtained. (1) The west boundary of the Late Triassic Ordos Basin was far beyond the scope of the current basin. The basin is connected with the Late Triassic Hexi Corridor Basin, and its western margin did not have tectonic characteristics of a foreland basin. (2) The Tianhuan depression was first formed in the Late Jurassic. At the late stage it was impacted by the late Yanshanian and Himalayan tectonic movement and the depression axis gradually moved eastwards to the present location with a cumulative migration distance of ~30 km. (3) Eastward migration of the depression axis caused adjustment and even destruction of the originally formed oil and gas reservoirs, so that oil and gas remigrated and aggregated, resulting in secondary structural reservoirs formed at high positions on the western flank of the depression. | LI Xiangbo LIU Huaqing WANYAN Rong WEI Lihua LIAO Jianbo FENG Ming MA Yuhu BAI Yunlai | 2009 | Acta Geologica Sinica(English Edition)2009，83，6： | 9 |
| 12 | Long-term eficacy of plasma-derived hepatitis B vaccine among Chinese children:a 12-year folow-up study显示文摘INTRODUCTIONToevaluatelongtermeficacyofaplasmaderivedhepatitisBvaccineandprovideevidencefordecisionmakingonthevaccineboost... | LIAO Su Su 1, LI Rong Cheng 2, LI Hui 1, YANG Jin Ye 2, ZENG Xian Jia 1, GONG Jian 2, WANG Shu Sheng 2, LI Yan Ping 2 and ZHANG Kong Lai 1 | 1999 | World Journal of Gastroenterology1999，5，2： | 9 |
| 13 | Fe_3O_4@C nanoparticles as high-performance Fenton-like catalyst for dye decoloration显示文摘Water pollution has become serious environmental problem nowadays. Advanced oxidation processes(AOP) have been widely applied in water treatment.However, traditional Fenton reaction based on Fe2﹢-H2O2 system has obvious drawbacks, which limit its applications In this study, magnetic Fe3O4core-C shell nanoparticles(Fe3O4@C NPs) were prepared for the decoloration of methylene blue(MB) via the co-precipitation followed by the hydrothermal dehydrogenation of glucose. Fe3O4@C NPs showed high catalytic activity of the decoloration of MB through the decomposition of H2O2 in Fenton-like reactions. Fe3O4@C NPs had much higher activity than bare Fe3O4 cores, suggesting the coating of carbon enhanced the catalytic activity. The performance of Fe3O4@C NPs was better at lower pH and higher temperature, but was significantly inhibited in the presence of radical scavenger tertiary butanol. Fe3O4@C NPs could be magnetic separated and regenerated, and maintained with very good catalytic activity. The implication for the applications of Fe3O4@C NP-catalyzed Fenton-like reactions in water treatment was discussed. | Xiaoliang Zhang Manli He Jia-Hui Liu Rong Liao Lianqin Zhao Jingru Xie Ruijue Wang Sheng-Tao Yang Haifang Wang Yuanfang Liu | 2014 | Chinese Science Bulletin2014，59，27： | 8 |
| 14 | Medical expenditure for esophageal cancer in China:a 10-year multicenter retrospective survey(2002-2011)显示文摘Background: Esophageal cancer is associated with substantial disease burden in China, and data on the economic burden are fundamental for setting priorities in cancer interventions. The medical expenditure for the diagnosis and treatment of esophageal cancer in China has not been fully quantified. This study aimed to examine the medical expenditure of Chinese patients with esophageal cancer and the associated trends.Methods: From 2012 to 2014, a hospital-based multicenter retrospective survey was conducted in 37 hospitals in 13 provinces/municipalities across China as a part of the Cancer Screening Program of Urban China. For each esophageal cancer patient diagnosed between 2002 and 2011, clinical information and expense data were extracted by using structured questionnaires. All expense data were reported in Chinese Yuan(CNY; 1 CNY = 0.155 USD) based on the2011 value and inflated using the year-specific health care consumer price index for China.Results: A total of 14,967 esophageal cancer patients were included in the analysis. It was estimated that the overall average expenditure per patient was 38,666 CNY, and an average annual increase of 6.27% was observed from 2002(25,111 CNY) to 2011(46,124 CNY). The average expenditures were 34,460 CNY for stage Ⅰ,39,302 CNY for stage Ⅱ,40,353 CNY for stage Ⅲ, and 37,432 CNY for stage IV diseases(P < 0.01). The expenditure also differed by the therapy type, which was 38,492 CNY for surgery, 27,933 CNY for radiotherapy, and 27,805 CNY for chemotherapy(P < 0.05).Drugs contributed to 45.02% of the overall expenditure.Conclusions: These conservative estimates suggested that medical expenditures for esophageal cancer in China substantially increased in the last 10 years, treatment for early-stage esophageal cancer costs less than that for advanced cases, and spending on drugs continued to account for a considerable proportion of the overall expenditure. | Lan-Wei Guo Hui-Yao Huang Ju-Fang Shi Li-Hong Lv Ya-Na Bai A-Yan Mao Xian-Zhen Liao Guo-Xiang Liu Jian-Song Ren Xiao-Jie Sun Xin-Yu Zhu Jin-Yi Zhou Ji-Yong Gong Qi Zhou Lin Zhu Yu-Qin Liu Bing-Bing Song Ling-Bin Du Xiao-Jing Xing Pei-An Lou Xiao-Hua Sun Xiao Qi Shou-Ling Wu Rong Cao Li Lan Ying Ren Kai Zhang Jie He Jian.Gong Zhang Min Dai | 2017 | Chinese Journal of Cancer2017，36，11： | 7 |
| 15 | Pathological and molecular examinations of postmortem testis biopsies reveal SARS-CoV-2 infection in the testis and spermatogenesis damage in COVID-19 patients显示文摘In late December 2019,the novel severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),which causes coronavirus disease 2019(COVID-19),was identified in Wuhan,China,and the ensuing pandemic has led to more than 50 million infected individuals and more than one million deaths by November 10,2020(https://covid19.who.int/).Pathologic investigations of autopsy tissue have focused primarily on the lung,heart,and kidney,whereas morphologic data on testis injury and the effects of SARS-CoV-2 infection on spermatogenesis are limited.Although two groups did not detect SARS-CoV-2 in the semen or testes of recovered COVID-19 patients,1,2 another group confirmed SARS-CoV-2 in the semen of patients.3 Therefore,it is currently unknown whether SARS-CoV-2 infection impacts spermatogenesis and male fertility.In the present study,we evaluated the effects of SARS-CoV-2 infection on spermatogenesis by examining the pathophysiology and molecular features of testes obtained from five male COVID-19 patients at autopsy. | Xixiang Ma Chuhuai Guan Rong Chen Yunyun Wang Shenglei Feng Rongshuai Wang Guoqiang Qu Sijia Zhao Fengli Wang Xiaoli Wang Dingyu Zhang Liang Liu Aihua Liao Shuiqiao Yuan | 2021 | Cellular & Molecular Immunology2021，18，2： | 6 |
| 16 | Formulation of dried lignans nanosuspension with high redispersibility to enhance stability,dissolution,and oral bioavailability显示文摘Herpetospermum caudigerum lignans(HTL), one of the potential drugs with anti-hepatitis B virus and hepatoprotective effects, has limited clinical applications because of poor aqueous solubility and low bioavailability. Both herpetrione(HPE) and herpetin(HPN) are the most abundant ingredients in HTL and exhibit weak acidity. The purpose of the present study was to produce dried preparations of HTL(composed of HPE and HPN) nanosuspensions(HTL-NS) with high redispersibility using lyophilization technology. The HTL-NS was prepared by utilizing precipitation-combined homogenization technology based on acid-base neutralization reactions, and critical formulation and process parameters affecting the characteristics of HTL-NS were optimized. The resultant products were characterized by particle size analysis, SEM, XRD, stability, solubility, dissolution and in vivo bioavailability. HTL-NS showed near-spherical-shaped morphology and the size was 243 nm with a narrow PDI value of 0.187. The dried preparations with a relatively large particle size of 286 nm and a PDI of 0.215 were achieved by using 4%(W/V) mannitol as cryoprotectants, and had a better stability at 4 or 25 oC for 2 months, compared to HTL-NS. In the in vitro test, the dried preparations showed markedly increased solubility and dissolution velocity. Besides, in the in vivo evaluation, it exhibited significant increases in AUC0–t, C_(max), MRT and a decrease in T_(max), compared to the raw drug.. In conclusion, our results provide a basis for the development of a drug delivery system for poorly water-soluble ingredients with p H-dependent solubility. | SHEN Gang CHENG Ling WANG Li-Qiang ZHANG Li-Hong SHEN Bao-De LIAO Wei-Bo LI Juan-Juan ZHENG Juan XU Rong YUAN Hai-Long | 2016 | Chinese Journal of Natural Medicines2016，14，10： | 5 |
| 17 | Effects of Different Microbes on Fermenting Feed for Sea Cucumber(Apostichopus japonicus)显示文摘The effects of different microbes on fermenting feed for sea cucumber(Apostichopus japonicus) were compared to select the optimal fermentation strain in this study. Saccharomgces cerevisae, Candida utilis, Bacillus subtilis and Geotrichum candidum were independently added into the experimental compound feed, while only saline was mixed with the control feed. The fermentation treatments were inoculated with 10% seed solution under the condition of 25℃ and 70% water content, which lasted for 5 days to elucidate the optimal microbe strain for fermenting effect. Physicochemical indexes and sensorial characteristics were measured per day during the fermentation. The indexes included dry matter recovery(DMR), crude protein(CP), the percentage of amino acid nitrogen to total nitrogen(AA-N/t N), the percentage of ammonia nitrogen to total nitrogen(NH3-N/t N), and the ratio of fermentation strains and vibrios to the total microbes, color, smell and viscosity. The results showed that DMR, CP and AA-N/t N of the S. cerevisae group reached the highest level on day 3, but the ratio of fermentation strain was second to C. utilis group. In addition, its NH3-N/t N and the ratio of vibrios were maintained at low levels, and the sensory evaluation score including smell, color and viscosity was the highest in S. cerevisae group on day 3. Therefore, S. cerevisae could be the optimal strain for the feed fermentation for sea cucumber. This research developed a new production method of fermentation feed for sea cucumber. | JIANG Yan WANG Yingeng MAI Kangsen ZHANG Zheng LIAO Meijie RONG Xiaojun | 2015 | Journal of Ocean University of China2015，14，5： | 5 |
| 18 | Characterization of the start-up behavior and steady-state performance of biotrickling filter removing low concentration toluene waste gas显示文摘A biotrickling filter packed with the regular ceramic spheres was designed and fabricated to purify the low toluene-concentration waste gas.Particular attention was made to the study of the start-up behavior of the biotrickling filter.Moreover,the steady performances were investigated to establish the experimental correlation between the operation factors and removal efficiency.It was found that the start-up process of the biotrickling filter exhibited three stages including the biofilm formation,biofilm development,and biofilm stabilization.The OD600 nm of the circulated liquid,gas pressure drop,elimination capacity,and temperature rise maintained at a rather low level in the biofilm formation stage,then increased rapidly in the biofilm development stage,and finally reached a stable value in the biofilm stabilization stage.It was also found that the removal efficiency of the biotrickling filter in the stable period decreased with increase in the waste gas flow rate,circulated liquid flow rate,and diameter of the ceramic sphere.More interestingly,it was revealed the operation modes have a significant influence on the removal efficiency of the biotrickling filter,and the microorganism show a high activity under the operating temperature ranging from 30°C to 40°C.The experimental correlations for describing the effect of operation factors on the removal efficiency of the biotrickling filters under both the co-current and counter-current operation modes were obtained.The correlation results are in good agreement with the experimental data. | CHEN Rong LIAO Qiang TIAN Xin WANG YongZhong ZHU Xun MIAO JunHe | 2012 | Science China(Technological Sciences)2012，55，6： | 4 |
| 19 | Palliative primary tumor resection provides survival beneits for the patients with metastatic colorectal cancer and low circulating levels of dehydrogenase and carcinoembryonic antigen显示文摘Background: It remains controversial whether palliative primary tumor resection(PPTR) can provide survival benefits to the patients with metastatic colorectal cancer(m CRC) who have unresectable metastases. The aim of this study was to evaluate whether PPTR could improve the survival of patients with m CRC.Methods: We conducted a retrospective study on consecutive m CRC patients with unresectable metastases who were diagnosed at Sun Yat?sen University Cancer Center in Guangzhou, Guangdong, China, between January 2005 and December 2012. Overall survival(OS) and progression?free survival(PFS) after first?line chemotherapy failure were compared between the PPTR and non?PPTR patient groups.Results: A total of 387 patients were identified, including 254 who underwent PPTR and 133 who did not. The median OS of the PPTR and non?PPTR groups was 20.8 and 14.8 months(P < 0.001), respectively. The median PFS after first?line chemotherapy was 7.3 and 4.8 months(P < 0.001) in the PPTR and non?PPTR groups, respectively. A larger proportion of patients in the PPTR group(219 of 254, 86.2%) showed local progression compared with that of patients in the non?PPTR group(95 of 133, 71.4%; P < 0.001). Only patients with normal lactate dehydrogenase(LDH) levels and with carcinoembryonic antigen(CEA) levels <70 ng/m L benefited from PPTR(median OS, 22.2 months for the PPTR group and 16.2 months for the non?PPTR group; P < 0.001).Conclusions: For m CRC patients with unresectable metastases, PPTR can improve OS and PFS after first?line chemo?therapy and decrease the incidence of new organ involvement. However, PPTR should be recommended only for patients with normal LDH levels and with CEA levels <70 ng/m L. | Wen-Zhuo He Yu-Ming Rong Chang Jiang Fang-Xin Liao Chen-Xi Yin Gui-Fang Guo Hui-Juan Qiu Bei Zhang Liang-Ping Xia | 2016 | Chinese Journal of Cancer2016，35，9： | 4 |
| 20 | Effect of Cold-Water Irrigation on Grain Quality Traits in japonica Rice Varieties from Yunnan Province,China显示文摘The response of grain quality traits to cold-water irrigation and its correlation with cold tolerance were studied in 11 japonica rice varieties from Yunnan Province, China. The results indicated that the response of grain quality traits to the cold-water stress varied with rice varieties and grain quality traits. Under the cold-water stress, grain width, chalky rice rate, whiteness, 1000-grain weight, brown rice rate, taste meter value, peak viscosity, trough viscosity, breakdown viscosity and final viscosity significantly decreased, whereas grain length-width ratio, head rice rate, alkali digestion value, protein content and setback viscosity markedly increased. However, the other traits such as grain length, amylose content, milled rice rate, peak viscosity time and pasting temperature were not significantly affected by the cold-water stress. Significant correlations were discovered between phenotypic acceptability and cold response indices of taste meter value, protein content, peak viscosity and breakdown viscosity. Therefore, it would be very important to improve the cold tolerance of Yunnan rice varieties in order to stabilize and improve their eating quality. | ZHAO Guo-zhen LIU Ji-xin YANG Sea-jun YEA Jong-doo LIAO Xin-hua Su Zhen-xi SHI Rong JIANG Cong DAI Lu-yuan | 2009 | Rice science2009，16，3： | 4 |
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