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| 1 | A novel m6A reader Prrc2a controls oligodendroglial specification and myelination显示文摘While N6-methyladenosine (m6A), the most abundant internal modification in eukaryotic mRNA, is linked to cell differentiation and tissue development, the biological significance of m6A modification in mammalian glial development remains unknown. Here, we identify a novel m6A reader, Prrc2a (Proline rich coiled-coil 2A), which controls oligodendrocyte specification and myelination. Nestin-Cre-mediated knockout of Prrc2a induces significant hypomyelination, decreased lifespan, as well as locomotive and cognitive defects in a mouse model. Further analyses reveal that Prrc2a is involved in oligodendrocyte progenitor cells (OPCs) proliferation and oligodendrocyte fate determination. Accordingly, oligodendroglial-lineage specific deletion of Prrc2a causes a similar phenotype of Nestin-Cre-mediated deletion. Combining transcriptome-wide RNA-seq, m6A-RIP-seq and Prrc2a RIP-seq analysis, we find that Olig2 is a critical downstream target gene of Prrc2a in oligodendrocyte development. Furthermore, Prrc2a stabilizes Olig2 mRNA through binding to a consensus GGACU motif in the Olig2 CDS (coding sequence) in an m6A-dependent manner. Interestingly, we also find that the m6A demethylase, Fto, erases the m6A modification of Olig2 mRNA and promotes its degradation. Together, our results indicate that Prrc2a plays an important role in oligodendrocyte specification through functioning as a novel m6A reader. These findings suggest a new avenue for the development of therapeutic strategies for hypomyelination-related neurological diseases. | Rong Wu Ang Li Baofa Sun Jian-Guang Sun Jinhua Zhang Ting Zhang Yusheng Chen Yujie Xiao Yuhao Gao Qingyang Zhang Jun Ma Xin Yang Yajin Liao Wei-Yi Lai Xiaolong Qi Shukun Wang Yousheng Shu Hai-Lin Wang Fengchao Wang Yun-Gui Yang Zengqiang Yuan | 2019 | Cell Research2019，29，1： | 32 |
| 2 | Standardized Operational Protocol for Human Brain Banking in China显示文摘With the progress of society, there is an increasing need to tackle disorders of the central nervous system. Human brain tissue, unlike animal tissues, is an irreplaceable resource for the study of neurological diseases (1)Aimed at scientific research and education, the roles of human brain tissue repositories are to acquire brain tissue from donors, prepare, process, and preserve collected samples,provide tissue to specific eligible facilities, and determine the characteristics of each tissue sample. | Wenying Qiu Hanlin Zhang Aimin Bao Keqing Zhu Yue Huang Xiaoxin Yan Jing Zhang Chunjiu Zhong Yong Shen Jiangning Zhou Xiaoying Zheng Liwei Zhang Yousheng Shu Beisha Tang Zhenxin Zhang Gang Wang Ren Zhou Bing Sun Changlin Gong Shumin Duan Chao Ma | 2019 | Neuroscience Bulletin2019，35，2： | 6 |
| 3 | Cell vibron polariton resonantly self-confined in the myelin sheath of nerve显示文摘Polaritons are arousing tremendous interests in physics and material sciences for their unique and amazing properties,especially including the condensation,lasing without inversion and even room-temperature superfluidity.Herein,we propose a cell vibron polariton(cell-VP):a collectively coherent mode of a photon and all phospholipid molecules in a myelin sheath formed by glial cells.Cell-VP can be resonantly self-confined in the myelin sheath under physiological conditions.The observations benefit from the specifically compact,ordered and polar thin-film structure of the sheath,and the relatively strong coupling of the mid-infrared photon with the vibrons of phospholipid tails in the myelin.The underlying physics is revealed to be the collectively coherent superposition of the photon and vibrons,the polariton induced significant enhancement of myelin permittivity,and the resonance of the polariton with the sheath.The captured cell-VPs in myelin sheaths may provide a promising way for super-efficient consumption of extra-weak bioenergy and even directly serve for quantum information.These findings further the understanding of nervous system operations at cellular level from the view of quantum mechanics. | Bo Song Yousheng Shu | 2020 | Nano Research2020，13，1： | 4 |
| 4 | Anterograde Trans-Synaptic Tagging Mediated by Adeno-Associated Virus显示文摘Trans-neuronal viral tracing is becoming one of the most important methods for mapping neuronal circuit connections,but an anterograde trans-synaptic tracer compatible with functional assays is still lacking.Adeno-associated virus(AAV)is replication-defective,and its production of offspring needs the help of adenovirus(AV)[1]or herpesvirus co-infection[2].The replication deficiency,non-pathogenicity,easy manipulability。 | Fei Zhao Hai-Fei Jiang Wen-Bo Zeng Yousheng Shu Min-Hua Luo Shumin Duan | 2017 | Neuroscience Bulletin2017，33，3： | 4 |
| 5 | Demonstration of biophoton-driven DNA replication via gold nanoparticle-distance modulated yield oscillation显示文摘Biologically,there exist two kinds of syntheses:photosynthesis and ATP-driven biosynthesis.The light harvesting of photosynthesis is known to achieve an efficiency of〜95%by the quantum energy transfer of photons.However,how the ATP-driven biosynthesis reaches its high efficiency still remains unknown.Deoxynucleotide triphosphates(dNTPs)in polymerase chain reaction(PCR)adopt the identical way of ATP to release their energy,and thus can be employed to explore the ATP energy process.Here,using a gold nanoparticle(AuNP)enhanced PCR(AuNP-PCR),we demonstrate that the energy released by phosphoanhydride-bond(PB)hydrolysis of dNTPs is in form of photons(PB-photons)to drive DNA replication,by modulating their resonance with the average inter-AuNP distance(D).The experimental results show that both the efficiency and yield of PCR periodically oscillate with D increasing,indicating a quantized process,but not simply a thermal one.The PB-photon wavelength is further determined to 8.4 pm.All these results support that the release,transfer and utilization of bioenergy are in the form of photons.Our findings of ATP-energy quantum conversion will open a new avenue to the studies of high-efficiency bioenergy utilization,biochemistry,biological quantum physics,and even brain sciences. | Na Li Daoling Peng Xianjing Zhang Yousheng Shu Feng Zhang Lei Jiang Bo Song | 2021 | Nano Research2021，14，1： | 4 |
| 6 | Laminar Distribution of Neurochemically-Identified Interneurons and Cellular Co-expression of Molecular Markers in Epileptic Human Cortex显示文摘Inhibitory GABAergic interneurons are fundamental elements of cortical circuits and play critical roles in shaping network activity. Dysfunction of interneurons can lead to various brain disorders, including epilepsy,schizophrenia, and anxiety. Based on the electrophysiological properties, cell morphology, and molecular identity,interneurons could be classified into various subgroups. In this study, we investigated the density and laminar distribution of different interneuron types and the coexpression of molecular markers in epileptic human cortex.We found that parvalbumin(PV) and somatostatin(SST)neurons were distributed in all cortical layers except layer I, while tyrosine hydroxylase(TH) and neuropeptide Y(NPY) were abundant in the deep layers and white matter.Cholecystokinin(CCK) neurons showed a high density in layers IV and VI. Neurons with these markers constituted*7.2%(PV), 2.6%(SST), 0.5%(TH), 0.5%(NPY), and4.4%(CCK) of the gray-matter neuron population. Doubleand triple-labeling revealed that NPY neurons were also SST-immunoreactive(97.7%), and TH neurons were more likely to express SST(34.2%) than PV(14.6%). A subpopulation of CCK neurons(28.0%) also expressed PV, but none contained SST. Together, these results revealed the density and distribution patterns of different interneuron populations and the overlap between molecular markers in epileptic human cortex. | Qiyu Zhu Wei Ke Quansheng He Xiongfei Wang Rui Zheng Tianfu Li Guoming Luan Yue-Sheng Long Wei-Ping Lia Yousheng Shu | 2018 | Neuroscience Bulletin2018，34，6： | 3 |
| 7 | Axonal bleb recording显示文摘Patch-clamp recording requires direct accessibility of the cell membrane to patch pipettes and allows the investigation of ion channel properties and functions in specific cellular compartments.The cell body and relatively thick dendrites are the most accessible compartments of a neuron,due to their large diameters and therefore great membrane surface areas.However,axons are normally inaccessible to patch pipettes because of their thin structure;thus studies of axon physiology have long been hampered by the lack of axon recording methods.Recently,a new method of patchclamp recording has been developed,enabling direct and tight-seal recording from cortical axons.These recordings are performed at the enlarged structure(axonal bleb) formed at the cut end of an axon after slicing procedures.This method has facilitated studies of the mechanisms underlying the generation and propagation of the main output signal,the action potential,and led to the finding that cortical neurons communicate not only in action potential-mediated digital mode but also in membrane potential-dependent analog mode. | Wenqin Hu Yousheng Shu | 2012 | Neuroscience Bulletin2012，28，4： | 3 |
| 8 | Functional Self-Excitatory Autapses(Auto-synapses)on Neocortical Pyramidal Cells显示文摘Synapses are essential structures in the nervous system and normally form between two cells.However,some occur in a single neuron between the axon and its own dendrites and soma.These special synapses are known as autapses(or autosynapses or self-synapses)[1].Previous studies have shown that autapses were much more abundant in cultured neurons[2–4]than in the intact brain,so there was suspicion that autapses in vivo may be wiring errors or redundant structures[5]. | Wei Ke Quansheng He Yousheng Shu | 2019 | Neuroscience Bulletin2019，35，6： | 2 |
| 9 | MAGNETOHYDRODYNAMIC PIPE FLOW IN DUCTS WITH PARTIAL CIRCULAR RING CROSS SECTION AND ANNULAR CROSS SECTION显示文摘In this paper we use the Green function method to solve the problem of steady one dimensionalflow of an incompressible viscous, electrically conducting fluid through a pipe with partial circular ring cross sec-tion and one with annular cross section, in the presence of an applied transverse uniform magnetic field, We ob-tain analytic solutions and carry out some numerical calculations of the velocity distribution and induced magnet-ic field. | Shu Yousheng, Department of Physics, Peking University, Beijing, ChinaQian Shangwu, Center of Theoretical Physics, CCAST (World Lab. ), Beijing Institute of Theoretical Physics, Academia Sinica, Beijing Department of Physics, Peking University, Beijing, China | 1990 | Acta Mechanica Sinica1990，6，1： | 0 |
| 10 | Functional Autapses Form in Striatal Parvalbumin Interneurons but not Medium Spiny Projection Neurons显示文摘Autapses selectively form in specific cell types in many brain regions.Previous studies have also found putative autapses in principal spiny projection neurons(SPNs)in the striatum.However,it remains unclear whether these neurons indeed form physiologically functional autapses.We applied whole-cell recording in striatal slices and identified autaptic cells by the occurrence of prolonged asynchronous release(AR)of neurotransmitters after bursts of high-frequency action potentials(APs).Surprisingly,we found no autaptic AR in SPNs,even in the presence of Sr^(2+).However,robust autaptic AR was recorded in parvalbumin(PV)-expressing neurons.The autaptic responses were mediated by GABA_(A) receptors and their strength was dependent on AP frequency and number.Further computer simulations suggest that autapses regulate spiking activity in PV cells by providing self-inhibition and thus shape network oscillations.Together,our results indicate that PV neurons,but not SPNs,form functional autapses,which may play important roles in striatal functions. | Xuan Wang Zhenfeng Shu Quansheng He Xiaowen Zhang Luozheng Li Xiaoxue Zhang Liang Li Yujie Xiao Bo Peng Feifan Guo Da-Hui Wang Yousheng Shu | 2023 | Neuroscience Bulletin2023，39，4： | 0 |
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