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| 1 | TRIM22 Inhibits the TRAF6-stimulated NF-κB Pathway by Targeting TAB2 for Degradation显示文摘Tripartite motif containing 22 (TRIM22), a member of the TRIM/RBCC family, has been reported to activate the nuclear factor-kappa B (NF-κB) pathway in unstimulated macrophage cell lines, but the detailed mechanisms governing this activation remains unclear. We investigated this mechanism in HEK293T cells. We found that overexpression of TRIM22 could activate the NF-κB pathway and conversely, could inhibit the tumor necrosis factor receptor-associated factor 6 (TRAF6)-stimulated NF-κB pathway in HEK293T cells. Further experiments showed that TRIM22 could decrease the self-ubiquitination of TRAF6, and interact with and degrade transforming growth factor-β activated kinase 1 binding protein 2 (TAB2), and that these effects could be partially rescued by a TRIM22 RING domain deletion mutant. Collectively, our data indicate that overexpression of TRIM22 may negatively regulate the TRAF6-stimulated NF-κB pathway by interacting with and degrading TAB2. | Hui Qiu Fang Huang Han Xiao Binlian Sun Rongge Yang | 2013 | Virologica Sinica2013，28，4： | 5 |
| 2 | Gene expression dynamics during the gonocyte to spermatogonia transition and spermatogenesis in the domestic yak显示文摘Background:Spermatogenesis is a cellular differentiation process that includes three major events:mitosis of spermatogonia,meiosis of spermatocytes and spermiogenesis.Steady-state spermatogenesis relies on functions of spermatogonial stem cells(SSCs).Establishing and maintaining a foundational SSC pool is essential for continued spermatogenesis in mammals.Currently,our knowledge about SSC and spermatogenesis is severely limited in domestic animals.Results:In the present study,we examined transcriptomes of testes from domestic yaks at four different stages(3,5,8 and 24 months of age)and attempted to identify genes that are associated with key developmental events of spermatogenesis.Histological analyses showed that the most advanced germ cells within seminiferous tubules of testes from 3,5,8 and 24 months old yaks were gonocytes,spermatogonia,spermatocytes and elongated spermatids,respectively.RNA-sequencing(RNA-seq)analyses revealed that 11904,4381 and 2459 genes were differentially expressed during the gonocyte to spermatogonia transition,the mitosis to meiosis transition and the meiosis to post-meiosis transition.Further analyses identified a list of candidate genes than may regulate these important cellular processes.CXCR4,a previously identified SSC niche factor in mouse,was one of the up-regulated genes in the 5 months old yak testis.Results of immunohistochemical staining confirmed that CXCR4 was exclusively expressed in gonocytes and a subpopulation of spermatogonia in the yak testis.Conclusions:Together,these findings demonstrated histological changes of postnatal testis development in the domestic yak.During development of spermatogonial lineage,meiotic and haploid germ cells are supported by dynamic transcriptional regulation of gene expression.Our transcriptomic analyses provided a list of candidate genes that potentially play crucial roles in directing the establishment of SSC and spermatogenesis in yak. | Guowen Wang Yongchang Li Qilin Yang Shangrong Xu Shike Ma Rongge Yan Ruina Zhang Gongxue Jia Deqiang Ai Qi’en Yang | 2019 | Journal of Animal Science and Biotechnology2019，10，4： | 3 |
| 3 | Drug resistance mutation profiles of the drug-nave and first-line regimen-treated HIV-1-infected population of Suzhou, China显示文摘Little is known about the prevalence of drug-resistant mutations in HIV-1-positive individuals in Suzhou, China. To elucidate the transmitted drug resistance(TDR) and acquired drug resistance mutation(ADR) profiles, we collected blood specimens from 127 drug-naive and 117 first-line drugtreated HIV-1-infected individuals sampled from 2014 to 2016 in Suzhou. We successfully amplified po/fragments from 100 drug-naive and 20 drug-treated samples. We then determined the drugresistant mutations to protease(PR) and reverse-transcriptase(RT) inhibitors according to the Stanford drug resistance database. Overall, 11 and 13 individuals had transmitted(drug-naive group) and acquired(treated group) resistance mutations, respectively. Six transmitted drugresistant mutations were found, including two mutations(L33F and L76V) in the protease region and four(K70N/E and V179D/E) in the RT region. Only L76 V was a major mutation, and K70N/E and V179D/E are known to cause low-level resistance to RT inhibitors. All 13 treated participants who had major drug resistance mutations demonstrated intermediate to high resistance to efavirenz and nevirapine, and six had a treatment duration of less than three months. No major mutations to RT inhibitors were found, implying that the epidemic of transmitted resistance mutations was not significant in this area. Our results suggest that more frequent virus load and drug resistance mutation tests should be conducted for individuals receiving antiretroviral treatment, especially for newly treated patients. Our research provides insights into the occurrence of HIV-1 drug resistance in Suzhou and will help to optimize the treatment strategy for this population. | Tingting Li Feng Qian Ting Yuan Weilu Xu Li Zhu Jinlong Huang Haiyan Wang Yueping Zhu Yinling Wang Xiaohong Li Saihong Gu Zhuqing Tan Hui Chen Xiangrong Luo Wei Zhu Weniuan Lu Ping Xu Ming Li Yuying Chen Yong Gao Rongge Yang Chuanwu Zhu Binlian Sun | 2017 | Virologica Sinica2017，32，4： | 2 |
| 4 | A numerical model of displacement for giant magnetos?trictive actuator显示文摘 | Yan Rongge Wang Bowen Yang Qingxin | 2004 | IEEE Transactions on Magnetics2004，14，2： | 1 |
| 5 | Geochronology and Geochemistry of Late Triassic Intrusive Rocks in the Xiuyan Area,Liaodong Peninsula,Eastern North China Craton:Petrogenesis and Implications for Lithospheric Thinning显示文摘The timing and mechanisms of lithospheric thinning and destruction of the North China Craton(NCC)remain controversial,and the overall geodynamics of the process are poorly understood.This paper documents Late Triassic igneous rocks including monzogranite,gabbro,and diorite from the Xiuyan District on the Liaodong Peninsula in the eastern NCC,which have LA-ICP-MS zircon U-Pb ages of 229.0±0.4 Ma,216.2±0.9 Ma,and 210.6±2.0 Ma,respectively.Monzogranite shows high-SiO_(2) adakite affinity,negative ε_(Hf)(t)values(-20.6 to-17.9),and old T_(DM2) ages(3.53-3.29 Ga),suggesting that their parental magma was derived from thickened Paleoarchean mafic lower crust and minor mantle materials that were also involved their generation.Gabbro is ultrapotassic,strongly enriched in LREEs and LILEs,depleted in HFSEs,and has evolved zircon Hf isotopes with negative ε_(Hf) of -10.04 to-5.85 and old T_(DM2) ages(2.59-2.22 Ga).These are diagnostic signatures of a crustal component,but their high contents of Mg O,Cr,Co,Ni indicate that the primary magma originated from enriched mantle.Diorite is enriched in LILEs and LREEs,depleted in HFSEs(with negative Nb,Ta,and Ti anomalies),and contains negative ε_(Hf)(t)values(-13.64 to-11.01).Compared with the gabbro,the diorite is relatively enriched in Nb,Ta and HREEs,and also contains younger T_(DM2) ages(2.11-1.94 Ga),suggesting that the diorite was formed by mixing between ancient lower crust-derived felsic magmas and asthenospheric mantle-derived magmas.Field observations,geochronology,geochemistry,and zircon Lu-Hf isotopes indicate that Late Triassic magmatism and tectonic activity resulted from deep subduction of the Yangtze Craton beneath the NCC in the Xiuyan area.This phase of tectonic activity was completed in the eastern NCC by the Late Triassic(216 Ma),and was subsequently followed by lithospheric thinning that began in the Late Triassic. | ZHANG Yanfei DONG Yang XIAO Rongge LIU Jingdang ZHAO Baoju | 2021 | Acta Geologica Sinica(English Edition)2021，95，5： | 1 |
| 6 | A Numerical Model of Displacement for Giant Magnetostrictive Actuator 显示文摘 | Rongge Yan Bowen Wang Qingxin Yang | 2004 | IEEE Transaction Applied Super Conductivity2004，14，2： | 1 |
| 7 | Inference of Global HIV-1 Sequence Patterns and Preliminary FeatureAnalysis显示文摘The epidemiology of HIV-1 varies in different areas of the world, and it is possible that this complexity may leave unique footprints in the viral genome. Thus, we attempted to find significant patterns in global HIV-1 genome sequences. By applying the rule inference algorithm RIPPER (Repeated Incremental Pruning to Produce Error Reduction) to multiple sequence alignments of Env sequences from four classes of compiled datasets, we generated four sets of signature patterns. We found that these patterns were able to distinguish southeastern Asian from non- southeastern Asian sequences with 97.5% accuracy, Chinese from non-Chinese sequences with 98.3% accuracy, African from non-African sequences with 88.4% accuracy, and southern African from non-southern African sequences with 91.2% accuracy. These patterns showed different associations with subtypes and with amino acid positions. In addition, some signature patterns were characteristic of the geographic area from which the sample was taken. Amino acid features corresponding to the phylogenetic clustering of HIV-1 sequences were consistent with some of the deduced patterns. Using a combination of patterns inferred from subtypes B, C, and all subtypes chimeric with CRF01_AE worldwide, we found that signature patterns of subtype C were extremely common in some sampled countries (for example, Zambia in southern Africa), which may hint at the origin of this HIV-1 subtype and the need to pay special attention to this area of Africa. Signature patterns of subtype B sequences were associated with different countries. Even more, there are distinct patterns at single position 21 with glycine, leucine and isoleucine corresponding to subtype C, B and all possible recombination forms chimeric with CRF01_AE, which also indicate distinct geographic features. Our method widens the scope of inference of signature from geographic, genetic, and genomic viewpoints. These findings may provide a valuable reference for epidemiological research or vaccine design. | Yan Wang Reda Rawi Daniel Hoffmann Binlian Sun Rongge Yang | 2013 | Virologica Sinica2013，28，4： | 1 |
| 8 | A magneto-mechanical strongly coupled model for giant magnetostrictive force sensor 显示文摘 | Qingxin Yang Rongge Yan Changzai Fan | 2007 | IEEE Transactions On Magnetics2007，43，4： | 1 |
| 9 | Magne- toelastic numerical analysis of permanent magnet synchronous motor including magnetostriction effects and harmonics显示文摘 | Lihua Zhu Qingxin Yang Rongge Yan | 2013 | IEEE Transactions on AppliedSupercon-ductivity2013，46，1： | 1 |
| 10 | A Magneto-mechanical Strongly Coupled Model for Giant Magnetostrictive Force Sensor显示文摘 | Yang Qingxin Yan Rongge Fan Changzai | | IEEE Transactions on Magnetics0，43，4： | 1 |
| 11 | Ficolin-2 binds to HIV-1 gp120 and blocks viral infection显示文摘Ficolin-2 is a lectin complement pathway activator present in normal human plasma and usually associated with infectious diseases, but little is known about the role of ficolin-2 in human immunodeficiency virus(HIV) infection. Here, we describe our novel findings that serum ficolin-2concentrations of 103 HIV-1 patients were much higher compared to those of 57 healthy donors. In vitro analysis showed that HIV-1 infection could enhance ficolin-2 expression. We further demonstrated that recombinant ficolin-2 protein could bind with HIV-1 envelope glycoprotein gp120, and subsequently induce complement dependent cytotoxicity. Moreover, ficolin-2 could block the entry of HIV-1 into target cells(TZM-b1 and MT-2 cells) and infection in a ficolin-2 dosedependent manner. To our knowledge, this is the first report about the protective role of ficolin-2against HIV-1 infection and our study suggests that ficolin-2 is an important human innate immune molecule against HIV. | Fengling Luo Tielong Chen Jun Liu Xihui Shen Yinnan Zhao Rongge Yang Xiaolian Zhang | 2016 | Virologica Sinica2016，31，5： | 1 |
| 12 | Numerical com- putation for a new way to reduce vibration and noise due to mag- netostriction and magnetic forces of transformer cores显示文摘 | ZHU Lihua YANG Qingxin YAN Rongge | 2013 | Journal of Applied Physics2013，113，17： | 1 |
| 13 | A Numerical Model of Displacement for Giant Magnetostrictive Actuator 显示文摘 | Rongge Yan Bowen Wang Qingxin Yang | 2004 | IEEE Transaction Applied Super Conductivity2004，14，2： | 1 |
| 14 | Differential selection in HIV-1 gp120 between subtype B and East Asian variant B'显示文摘HIV-1 evolves strongly and undergoes geographic differentiation as it spreads in diverse host populations around the world.For instance,distinct genomic backgrounds can be observed between the pandemic subtype B,prevalent in Europe and North-America,and its offspring clade B' in East Asia.Here we ask whether this differentiation affects the selection pressure experienced by the virus.To answer this question we evaluate selection pressure on the HIV-1 envelope protein gp120at the level of individual codons using a simple and fast estimation method based on the ratio k_alk_s of amino acid changes to synonymous changes.To validate the approach we compare results to those from a state-of-the-art mixed-effect method.The agreement is acceptable,but the analysis also demonstrates some limitations of the simpler approach.Further,we find similar distributions of codons under stabilizing and directional selection pressure in gp120 for subtypes B and B' with more directional selection pressure in variable loops and more stabilizing selection in the constant regions.Focusing on codons with increased k_alk_s values in B',we show that these codons are scattered over the whole of gp120,with remarkable clusters of higher density in regions flanking the variable loops.We identify a significant statistical association of glycosylation sites and codons with increased k_alk_s values. | Stefan Dang Yan Wang Bettina Budeus Jens Verheyen Rongge Yang Daniel Hoffmann | 2014 | Virologica Sinica2014，29，1： | 0 |
| 15 | The V1 region of gp120 is preferentially selected during SIV/HIV transmission and is indispensable for envelope function and virus infection显示文摘A transmission bottleneck occurs during each human immunodeficiency virus(HIV) transmission event, which allows only a few viruses to establish new infection. However, the genetic characteristics of the transmitted viruses that are preferentially selected have not been fully elucidated. Here, we analyzed amino acids changes in the envelope protein during simian immunodeficiency virus(SIV)/HIV deep transmission history and current HIV evolution within the last 15–20 years. Our results confirmed that the V1V2 region of gp120 protein, particularly V1, was preferentially selected. A shorter V1 region was preferred during transmission history, while during epidemic, HIV may evolve to an expanded V1 region gradually and thus escape immune recognition. We then constructed different HIV-1 V1 mutants using different HIV-1 subtypes to elucidate the role of the V1 region in envelope function. We found that the V1 region, although highly variable, was indispensable for virus entry and infection, probably because V1 deletion mutants exhibited impaired processing of gp160 into mature gp120 and gp41. Additionally, the V1 region affected Env incorporation. These results indicated that the V1 region played a critical role in HIV transmission and infection. | Yanpeng Li Ulf Dittmer Yan Wang Jiping Song Binlian Sun Rongge Yang | 2016 | Virologica Sinica2016，31，3： | 0 |
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