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| 1 | Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases显示文摘在各种各样的纸巾的 microRNAs (miRNAs ) 的 Dysregulated 表示与许多疾病被联系了,包括癌症。这里,我们证明 miRNAs 在象老鼠,老鼠,牛的胎儿,小牛,和马那样的人和另外的动物的浆液和血浆是在场的。在浆液的 miRNAs 的层次在一样的种类的个人之中稳定、可再现、一致。采用 Solexa,我们定序健康中国题目的所有浆液 miRNAs 并且分别地在男、女的题目发现超过 100 和 91 浆液 miRNAs。我们也为肺癌症, colorectal 癌症,和糖尿病识别了浆液 miRNAs 的特定的表示模式,提供证据那浆液 miRNAs 为各种各样的疾病包含指纹。癌症特定的浆液 miRNAs 由 Solexa 获得了的二个非小的房间肺进一步在 75 个健康施主和 152 个癌症病人的独立审判被验证,用量的反向的抄写聚合酶链反应试金。通过这些分析,我们断定浆液 miRNAs 能为各种各样的癌症和另外的疾病的察觉用作潜在的 biomarkers。 | Xi Chen Yi Ba Lijia Ma Xing Cai Yuan Yin Kehui Wang Jig ang Guo Yujing Zhang Jiangning Chen Xing Guo Qibin Li Xiaoying Li Wenjing Wang Yan Zhang Jin Wang Xueyuan Jiang Yang Xiang Chen Xu Pingping Zheng Juanbin Zhang Ruiqiang Li Hongjie Zhang Xiaobin Shang Ting Gong Guang Ning Jun Wang Ke Zen Junfeng Zhang Chen-Yu Zhang | 2008 | Cell Research2008，18，10： | 975 |
| 2 | The Tea Tree Genome Provides Insights into Tea Flavor and Independent Evolution of Caffeine Biosynthesis显示文摘茶是世界有巨大的经济、药用、文化的重要性的最旧、很流行的包含咖啡因的饮料。这里,我们在场第一个高质量的核苷酸序列充满重复(80.9%) ,栽培的茶树山茶 sinensis 的 3.02-Gb 染色体。我们证明茶树的一种非常地大的染色体尺寸从一些 LTR retrotransposon 家庭的慢、稳定、长期的扩大被结果。除了一个最近的整个染色体的复制事件,基因的系特定的扩大把新陈代谢的生合成与 flavonoid 联系了被发现,它提高 catechin 生产,萜烯酶激活,和应力公差,为茶风味和改编的重要特征。我们相对可可子和咖啡表明一个独立人士和茶咖啡因合成小径的快速的进化。在 25 山茶种类之中的比较研究表明那更高的表情大多数 flavonoid- 和咖啡因铺平 -- 然而并非 theanine 相关的基因贡献 catechins 和咖啡因的增加的生产并且因此提高处理茶的适用性和茶质量。这些新奇调查结果为进一步的 metabolomic 和典型生合成小径的功能的 genomic 精炼铺平道路并且将帮助开发将最后满足并且吸引世界范围的更多的茶喝酒者的茶风味的一个更多样化的集合。 | En-Hua Xia Hai-Bin Zhang Jun Sheng Kui Li Qun-Jie Zhang Changhoon Kim Yun Zhang Yuan Liu Ting Zhu Wei Li Hui Huang Yan Tong Hong Nan Cong Shi Chao Shi Jian-Jun Jiang Shu-Yan Mao Jun-Ying Jiao Dan Zhang Yuan Zhao You-Jie Zhao Li-Ping Zhang Yun-Long Liu Ben-Ying Liu Yue Yu Sheng-Fu Shao De-Jiang Ni Evan E. Eichler Li-Zhi Gao | 2017 | Molecular Plant2017，10，6： | 113 |
| 3 | Transmission Dynamics of an Outbreak of the COVID-19 Delta Variant B.1.617.2——Guangdong Province,China,May-June 2021显示文摘On May 21,2021,a local case of coronavirus disease 2019(COVID-19)was confirmed in a 75-year-old woman(experienced onset of symptoms on May 18)in Liwan District,Guangzhou City,Guangdong Province,China.The number of infections has increased in the following 10 days and led to 5 generations of transmission. | Meng Zhang Jianpeng Xiao Aiping Deng Yingtao Zhang Yali Zhuang Ting Hu Jiansen Li Hongwei Tu Bosheng Li Yan Zhou Jun Yuan Lei Luo Zimian Liang Youzhi Huang Guoqiang Ye Mingwei Cai Gongli Li Bo Yang Bin Xu Ximing Huang Yazun Cui Dongsheng Ren Yanping Zhang Min Kang Yan Li | 2021 | China CDC weekly2021，3，27： | 51 |
| 4 | Massive individual orbital angular momentum channels for multiplexing enabled by Dammann gratings显示文摘Data transmission rates in optical communication systems are approaching the limits of conventional multiplexing methods.Orbital angular momentum(OAM)in optical vortex beams offers a new degree of freedom and the potential to increase the capacity of free-space optical communication systems,with OAM beams acting as information carriers for OAM division multiplexing(OAM-DM).We demonstrate independent collinear OAM channel generation,transmission and simultaneous detection using Dammann optical vortex gratings(DOVGs).We achieve 80/160 Tbit s^(-1) capacity with uniform power distributions along all channels,with 1600 individually modulated quadrature phase-shift keying(QPSK)/16-QAM data channels multiplexed by 10 OAM states,80 wavelengths and two polarizations.DOVG-enabled OAM multiplexing technology removes the bottleneck of massive OAM state parallel detection and offers an opportunity to raise optical communication systems capacity to Pbit s^(-1) level. | Ting Lei Meng Zhang Yuru Li Ping Jia Gordon Ning Liu Xiaogeng Xu Zhaohui Li Changjun Min Jiao Lin Changyuan Yu Hanben Niu Xiaocong Yuan | 2015 | Light(Science & Applications)2015，4，1： | 47 |
| 5 | The Relationship between Glucose Excursion and Cognitive Function in Aged Type 2 Diabetes Patients显示文摘Objective Evidence suggests that type 2 diabetes (T2DM) is associated with an increased risk of dementia and that glucose variability is an independent risk factor for diabetic complications.This study investigated the relationship between glucose excursion and cognitive function in aged T2DM patients.Methods A total of 248 aged T2DM patients wore a continuous glucose monitoring system (CGMS) for 3 days in order to evaluate glucose excursion,including mean amplitude of glycemic excursions (MAGE) and mean of daily difference (MODD).All subjects were evaluated with a number of accepted cognitive function tests,including themini‐mental status examination (MMSE).The relationship between MAGE and MODD and performance on these cognitive tests was assessed.Results The MAGE and MMSE score were negatively correlated,likewise with the correlation between MODD and MMSE.Liner multivariate regression analysis showed that MAGE and MODD were also negatively related to MMSE independent of age,sex,glycemic control,hypertension,smoking,or coronary heart disease history.Conclusion Glucose excursion is related to cognitive function in aged T2DM patients.Elevated glucose excursion decreased the MMSE score,which reflects general cognitive function.Thus,therapy aimed at controlling glucose excursion may be beneficial for maintaining cognitive function in aged T2DM patients. | ZHONG Yuan ZHANG Xiao Yan MIAO Ya ZHU Jie Hua YAN Hong WANG Bei Yun JIN Jun HU Ting Jun JIA Wei Ping | 2012 | Biomedical and Environmental Sciences2012，25，1： | 34 |
| 6 | A novel m6A reader Prrc2a controls oligodendroglial specification and myelination显示文摘While N6-methyladenosine (m6A), the most abundant internal modification in eukaryotic mRNA, is linked to cell differentiation and tissue development, the biological significance of m6A modification in mammalian glial development remains unknown. Here, we identify a novel m6A reader, Prrc2a (Proline rich coiled-coil 2A), which controls oligodendrocyte specification and myelination. Nestin-Cre-mediated knockout of Prrc2a induces significant hypomyelination, decreased lifespan, as well as locomotive and cognitive defects in a mouse model. Further analyses reveal that Prrc2a is involved in oligodendrocyte progenitor cells (OPCs) proliferation and oligodendrocyte fate determination. Accordingly, oligodendroglial-lineage specific deletion of Prrc2a causes a similar phenotype of Nestin-Cre-mediated deletion. Combining transcriptome-wide RNA-seq, m6A-RIP-seq and Prrc2a RIP-seq analysis, we find that Olig2 is a critical downstream target gene of Prrc2a in oligodendrocyte development. Furthermore, Prrc2a stabilizes Olig2 mRNA through binding to a consensus GGACU motif in the Olig2 CDS (coding sequence) in an m6A-dependent manner. Interestingly, we also find that the m6A demethylase, Fto, erases the m6A modification of Olig2 mRNA and promotes its degradation. Together, our results indicate that Prrc2a plays an important role in oligodendrocyte specification through functioning as a novel m6A reader. These findings suggest a new avenue for the development of therapeutic strategies for hypomyelination-related neurological diseases. | Rong Wu Ang Li Baofa Sun Jian-Guang Sun Jinhua Zhang Ting Zhang Yusheng Chen Yujie Xiao Yuhao Gao Qingyang Zhang Jun Ma Xin Yang Yajin Liao Wei-Yi Lai Xiaolong Qi Shukun Wang Yousheng Shu Hai-Lin Wang Fengchao Wang Yun-Gui Yang Zengqiang Yuan | 2019 | Cell Research2019，29，1： | 32 |
| 7 | Saponins from Panax japonicus attenuate age-related neuroinflammation via regulation of the mitogen-activated protein kinase and nuclear factor kappa B signaling pathways显示文摘Neuroinflammation is recognized as an important pathogenic factor for aging and related cognitive disorders. Mitogen-activated protein kinase and nuclear factor kappa B signaling pathways may mediate neuroinflammation. Saponins from Panax japonicus are the most abundant and bioactive members in rhizomes of Panax japonicus, and show anti-inflammatory activity. However, it is not known whether saponin from Panax japonicus has an anti-inflammatory effect in the aging brain, and likewise its underlying mechanisms. Sprague-Dawley rats were divided into control groups(3-, 9-, 15-, and 24-month-old groups) and saponins from Panax japonicus-treated groups. Saponins from Panax japonicus-treated groups were orally administrated saponins from Panax japonicus at three doses of 10, 30, and 60 mg/kg once daily for 6 months until the rats were 24 months old. Immunohistochemical staining and western blot assay results demonstrated that many microglia were activated in 24-month-old rats compared with 3-and 9-month-old rats. Expression of interleukin-1β, tumor necrosis factor-α, cyclooxygenase-2, and inducible nitric oxide synthase increased. Each dose of saponins from Panax japonicus visibly suppressed microglial activation in the aging rat brain, and inhibited expression levels of the above factors. Each dose of saponins from Panax japonicus markedly diminished levels of nuclear factor kappa B, IκBα, extracellular signal-regulated kinase, c-Jun N-terminal kinase, and p38. These results confirm that saponins from Panax japonicus can mitigate neuroinflammation in the aging rat brain by inhibition of the mitogen-activated protein kinase and nuclear factor kappa B signaling pathways. | Li-li Deng Ding Yuan Zhi-yong Zhou Jing-zhi Wan Chang-cheng Zhang Chao-qi Liu Yao-yan Dun Hai-xia Zhao Bo Zhao Yuan-jian Yang Ting Wang | 2017 | Neural Regeneration Research2017，12，11： | 17 |
| 8 | Characterization of Xanthomonas oryzae- Responsive cis-Acting Element in the Promoter of Rice Race-Specific Susceptibility Gene Xa13显示文摘米饭 Xa13 基因,其倡导者怀有 UPT (由抄写起来调整像使活跃之物[TAL ] 受动器) 盒子, UPTPthXo1,在 Xanthomonas oryzae pv 引起的赛跑特定的致病力起一个枢轴的作用。oryzae (Xoo ) 种类 PXO99。PXO99 由导致 Xa13 引起米饭疾病。然而, UPTPthXo1 框是否是在 Xa13 表示的激活的唯一的 PXO99 应答的调整 cis 元素,是未知的。我们在米饭和 PXO99 蛋白质的绑定分析了一系列截断结束、截断地点、变异地点的 Xa13 倡导者的表达式到未经触动、部分,或变异地点的 UPTPthXo1 框。在 Xa13 倡导者, UPTPthXo1 盒子是导致导致 PXO99 的 Xa13 表示的唯一的 Xoo 应答的行动 cis 元素。5'-terminal 框的第二,第三,和第四核苷酸为细菌的蛋白质绑定和基因激活是重要的;任何一个这些地点的变化废除了导致 PXO99 的基因表示。而且, UPTPthXo1 盒子的 3'-half 也为蛋白质绑定和基因激活被要求。这些调查结果将经由 UPT 盒子和 TAL 受动器提高我们米饭和 Xoo 的相互作用的分子的机制的理解。 | Ting Yuan Xianghua Li Jinghua Xiao Shiping Wang | 2011 | Molecular Plant2011，4，2： | 16 |
| 9 | Progress of the key materials for organic solar cells显示文摘Organic solar cells have attracted academic and industrial interests due to the advantages like lightweight,flexibility and roll-to-roll fabrication.Nowadays,18%power conversion efficiency has been achieved in the state-of-the-art organic solar cells.The recent rapid progress in organic solar cells relies on the continuously emerging new materials and device fabrication technologies,and the deep understanding on film morphology,molecular packing and device physics.Donor and acceptor materials are the key materials for organic solar cells since they determine the device performance.The past 25 years have witnessed an odyssey in developing high-performance donors and acceptors.In this review,we focus on those star materials and milestone work,and introduce the molecular structure evolution of key materials.These key materials include homopolymer donors,D-A copolymer donors,A-D-A small molecular donors,fullerene acceptors and nonfullerene acceptors.At last,we outlook the challenges and very important directions in key materials development. | Yang Tong Zuo Xiao Xiaoyan Du Chuantian Zuo Yuelong Li Menglan Lv Yongbo Yuan Chenyi Yi Feng Hao Yong Hua Ting Lei Qianqian Lin Kuan Sun Dewei Zhao Chunhui Duan Xiangfeng Shao Wei Li Hin-Lap Yip Zhengguo Xiaol Bin Zhang Qingzhen Bian Yuanhang Cheng Shengjian Liu Ming Cheng Zhiwen Jin Shangfeng Yang Liming Ding | 2020 | Science China Chemistry2020，63，6： | 15 |
| 10 | Recombinant CC16 protein inhibits the production of pro-inflammatory cytokines via NF-KB and p38 MAPK pathways in LPS-activated RAW264,7 macrophages显示文摘积累的证据显示克莱拉房间 protein-16 (CC16 ) 有反煽动性的功能,尽管深奥分子的小径完全没被阐明。这里,我们评估了效果肿瘤坏死因素 alpha (TNF-) 的表示上的 recombinant 老鼠 CC16 (rCC16 ) , interleukin-6 (IL-6 ) ,和在 lipopolysaccharide (LPS ) 的 IL-8 刺激了老鼠巨噬细胞(RAW264.7 房间) 并且探索了内在的分子的机制。rCC16 在两个禁止了导致 LPS 的 TNF- , IL-6,和 IL-8 表示,这被发现送信人 ribonucleicacid (mRNA ) 水平和蛋白质以一种集中依赖者方式铺平,由即时反向的 transcriptase 聚合酶链反应和连接酶的 immunosorbent 试金示威了。如此的镇压效果被 transcriptional 活动和原子因素(NF ) 的脱氧核糖核酸酸绑定活动的抑制伴随 -B 然而并非使活跃之物蛋白质(AP )-1。西方的污点分析进一步表明 rCC16 禁止了原子 NF-B 和 NF-B 禁止的蛋白质 IB,和 p38 的 cytosolic NF-B, phosphorylation 和减小的减小的增加激活 mitogen 的蛋白质 kinase (MAPK ) 依赖者由在它的 p65 子单元的 Ser276 的 phosphorylation 的 NF-B 激活。而且, rCC16 被发现没另外在c6月N终端 kinase ,c6月,或c-Jun.的原子 translocation 的 phosphorylation 上有效果,TNF-的减小, IL-6 ,并且 IL-8 被颠倒内长的uteroglobin有约束力的蛋白质的水平什么时候被 RNA 干扰在 对待rCC16 、 对待LPS 的 RAW264.7 房间减少。我们的数据建议 rCC16 由在 RAW264.7 房间使 NF-B 和 p38 MAPK 然而并非 AP-1 失去活性压制调停 LPS 的煽动性的调停人 TNF- , IL-6,和 IL-8 生产。 | Min Pang Yangyang Yuan Dong Wang Ting Li Dan Wang Xiaohong Shi Min Guo Chunfang Wang Xinri Zhang Guoping Zheng Baofeng Yu Hailong Wang | 2017 | Acta Biochimica et Biophysica Sinica2017，49，5： | 14 |
| 11 | COVID-19: Challenges to GIS with Big Data显示文摘The outbreak of the 2019 novel coronavirus disease(COVID-19)has caused more than 100,000 people infected and thousands of deaths.Currently,the number of infections and deaths is still increasing rapidly.COVID-19 seriously threatens human health,production,life,social functioning and international relations.In the fight against COVID-19,Geographic Information Systems(GIS)and big data technologies have played an important role in many aspects,including the rapid aggregation of multi-source big data,rapid visualization of epidemic information,spatial tracking of confirmed cases,prediction of regional transmission,spatial segmentation of the epidemic risk and prevention level,balancing and management of the supply and demand of material resources,and socialemotional guidance and panic elimination,which provided solid spatial information support for decision-making,measures formulation,and effectiveness assessment of COVID-19 prevention and control.GIS has developed and matured relatively quickly and has a complete technological route for data preparation,platform construction,model construction,and map production.However,for the struggle against the widespread epidemic,the main challenge is finding strategies to adjust traditional technical methods and improve speed and accuracy of information provision for social management.At the data level,in the era of big data,data no longer come mainly from the government but are gathered from more diverse enterprises.As a result,the use of GIS faces difficulties in data acquisition and the integration of heterogeneous data,which requires governments,businesses,and academic institutions to jointly promote the formulation of relevant policies.At the technical level,spatial analysis methods for big data are in the ascendancy.Currently and for a long time in the future,the development of GIS should be strengthened to form a data-driven system for rapid knowledge acquisition,which signifies ts that GIS should be used to reinforce the social operation parameterization of models and methods,especially when providing support for social management. | Chenghu Zhou Fenzhen Su Tao Pei An Zhang Yunyan Du Bin Luo Zhidong Cao Juanle Wang Wen Yuan Yunqiang Zhu Ci Song Jie Chen Jun Xu Fujia Li Ting Ma Lili Jiang Fengqin Yan Jiawei Yi Yunfeng Hu Yilan Liao Han Xiao | 2020 | Geography and Sustainability2020，1，1： | 13 |
| 12 | Integrated(de)multiplexer for orbital angular momentum fiber communication显示文摘The quickly increasing data transfer load requires an urgent revolution in current optical communication. Orbital angular momentum(OAM) multiplexing is a potential candidate with its ability to considerably enhance the capacity of communication. However, the lack of a compact, efficient, and integrated OAM(de)multiplexer prevents it from being widely applied. By attaching vortex gratings onto the facets of a few-mode fiber, we demonstrate an integrated fiber-based OAM(de)multiplexer. A vortex grating fabricated on the fiber facet enables the direct multiplexing of OAM states at one port and the demultiplexing of OAM states at the other port. The measured bit error rate of the carrier signal after propagating through a 5-km few-mode fiber confirms the validity and effectiveness of the proposed approach. The scheme offers advantages in future high-capacity OAM communication based on optical fiber. | ZHENWEI XIE SHECHENG GAO TING LEI SHENGFEI FENG YAN ZHANG FAN LI JIANBO ZHANG ZHAOHUI LI XIAOCONG YUAN | 2018 | Photonics Research2018，6，7： | 13 |
| 13 | 2018: The Hottest Summer in China and Possible Causes显示文摘In 2018,China experienced the hottest summer since 1961.The maximum,mean,and minimum temperatures all reached the highest.Air temperatures in most regions were much higher than normal;in northern China especially,the temperature anomalies were above double of the standard deviations.Consistent variations of temperature anomalies appeared in the national mean and in northern China on different timescales from intraseasonal to annual,indicating that the above normal temperature in northern China contributed significantly to the record-breaking hot summer of entire China.Relationships among the high temperature in summer 2018,the tropospheric circulation,and the global sea surface temperatures(SSTs)are further analyzed.It is found that the intensified and more northward western Pacific subtropical high(WPSH),weakened Northeast China cold vortex(NECV),and positive geopotential height anomaly from northern China to the Sea of Japan resulted in the abnormally high temperature in summer 2018.From late July to mid August,the WPSH was stronger than normal,with its ridge line jumping to north of 40°N;meanwhile,the NECV was much weaker and more northward than normal;both of the two systems led to the persistent high temperature in northern China during this period.In addition,the SSTs in Kuroshio and its extension area(K–KE)in summer 2018 were also the highest since 1961 and the greatest positive SST anomaly in K–KE was favorable for the above normal geopotential height over North China–Northeast China–Japan at 500 hPa,giving rise to the exceptionally high temperature in northern China. | Ting DING Yuan YUAN Jianming ZHANG Hui GAO | 2019 | Journal of Meteorological Research2019，33，4： | 13 |
| 14 | Management of granulomatous lobular mastitis: an international multidisciplinary consensus(2021 edition)显示文摘Granulomatous lobular mastitis(GLM) is a rare and chronic benign inflammatory disease of the breast. Difficulties exist in the management of GLM for many front-line surgeons and medical specialists who care for patients with inflammatory disorders of the breast. This consensus is summarized to establish evidence-based recommendations for the management of GLM. Literature was reviewed using PubMed from January 1, 1971 to July 31, 2020. Sixty-six international experienced multidisciplinary experts from 11 countries or regions were invited to review the evidence.Levels of evidence were determined using the American College of Physicians grading system, and recommendations were discussed until consensus. Experts discussed and concluded 30 recommendations on historical definitions,etiology and predisposing factors, diagnosis criteria, treatment, clinical stages, relapse and recurrence of GLM. GLM was recommended as a widely accepted definition. In addition, this consensus introduced a new clinical stages and management algorithm for GLM to provide individual treatment strategies. In conclusion, diagnosis of GLM depends on a combination of history, clinical manifestations, imaging examinations, laboratory examinations and pathology.The approach to treatment of GLM should be applied according to the different clinical stage of GLM. This evidencebased consensus would be valuable to assist front-line surgeons and medical specialists in the optimal management of GLM. | Qian-Qian Yuan Shu-Yuan Xiao Omar Farouk Yu-Tang Du Fereshte Sheybani Qing Ting Tan Sami Akbulut Kenan Cetin Afsaneh Alikhassi Rami Jalal Yaghan Irmak Durur-Subasi Fatih Altintoprak Tae Ik Eom Fatih Alper Mustafa Hasbahceci David Martínez-Ramos Pelin Seher Oztekin Ava Kwong Cedric W.Pluguez-Turul Kirstyn EBrownson Shirish Chandanwale Mehran Habib Liu-Yi Lan Rui Zhou Xian-Tao Zeng Jiao Bai Jun-Wen Bai Qiong-Rong Chen Xing Chen Xiao-Ming Zha Wen-Jie Dai Zhi-Jun Dai Qin-Yu Feng Qing-Jun Gao Run-Fang Gao Bao-San Han Jin-Xuan Hou Wei Hou Hai-Ying Liao Hong Luo Zheng-Ren Liu Jing-Hua Lu Bin Luo Xiao-Peng Ma Jun Qian Jian-Yong Qin Wei Wei Gang Wei Li-Ying Xu Hui-Chao Xue Hua-Wei Yang Wei-Ge Yang Chao-Jie Zhang Fan Zhang Guan-Xin Zhang Shao-Kun Zhang Shu-Qun Zhang Ye-Qiang Zhang Yue-Peng Zhang Sheng-Chu Zhang Dai-Wei Zhao Xiang-Min Zheng Le-Wei Zheng Gao-Ran Xu Wen-Bo Zhou Gao-Song Wu | 2022 | Military Medical Research2022，9，4： | 13 |
| 15 | Redistribution of Pb, Zn and Cu Fractions in Tailing Soils Treated with Different Extractants显示文摘The efficiency of EDTA, HNO3 and CaCl2 as extractants to remove Pb, Zn and Cu from tailing soils without varying soil pH was investigated with distributions of Pb, Zn and Cu being determined before and after extraction using the sequential extraction procedure of the optimized European Community Bureau of Reference (BCR). Results indicated that EDTA and HNO3 were both effective extracting agents.The extractability of extractants for Pb and Zn was in the order EDTA > HNO3 > CaCl2, while for Cu it was HNO3 > EDTA > CaCl2. After EDTA extraction, the proportion of Pb, Zn and Cu in the four fractions varied greatly, which was related to the strong extraction and complexation ability. Before and after extraction with HNO3 and CaCl2, the percentages of Pb, Zn and Cu in the reducible, oxidizable and residual fractions changed little compared to the acid-extractable fraction. The lability of metal in the soil and the kinds of extractants were the factors controlling the effects of metal extraction. | LIU Yun-Guo WANG Xian-Hai ZENG Guang-Ming LI Xin ZHOU Chun-Hua FAN Ting LI Yong-Li YUAN Xin-Zhong | 2006 | Pedosphere2006，16，3： | 12 |
| 16 | Potentiating CD8^(+) T cell antitumor activity by inhibiting PCSK9 to promote LDLR-mediated TCR recycling and signaling显示文摘Metabolic regulation has been proven to play a critical role in T cell antitumor immunity.However,cholesterol metabolism as a key component of this regulation remains largely unexplored.Herein,we found that the low-density lipoprotein receptor(LDLR),which has been previously identified as a transporter for cholesterol,plays a pivotal role in regulating CD8+T cell antitumor activity.Besides the involvement of cholesterol uptake which is mediated by LDLR in T cell priming and clonal expansion,we also found a non-canonical function of LDLR in CD8+T cells:LDLR interacts with the T-cell receptor(TCR)complex and regulates TCR recycling and signaling,thus facilitating the effector function of cytotoxic T-lymphocytes(CTLs).Furthermore,we found that the tumor microenvironment(TME)downregulates CD8+T cell LDLR level and TCR signaling via tumor cell-derived proprotein convertase subtilisin/kexin type 9(PCSK9)which binds to LDLR and prevents the recycling of LDLR and TCR to the plasma membrane thus inhibits the effector function of CTLs.Moreover,genetic deletion or pharmacological inhibition of PCSK9 in tumor cells can enhance the antitumor activity of CD8+T cells by alleviating the suppressive effect on CD8+T cells and consequently inhibit tumor progression.While previously established as a hypercholesterolemia target,this study highlights PCSK9/LDLR as a potential target for cancer immunotherapy as well. | Juanjuan Yuan Ting Cai Xiaojun Zheng Yangzi Ren Jingwen Qi Xiaofei Lu Huihui Chen Huizhen Lin Zijie Chen Mengnan Liu Shangwen He Qijun Chen Siyang Feng Yingjun Wu Zhenhai Zhang Yanqing Ding Wei Yang | 2021 | Protein & Cell2021，12，4： | 12 |
| 17 | The Effects of Demographic Features on Differences in Sensitivity between PCL-C and SCL-90 Scores in a Follow-up Study in Secondary School Students in the Wenchuan Earthquake Region显示文摘Objective To analyze the sensitivity of effect factors between the PCL‐C and the SCL‐90,to provide evidence for social psychological crisis screening and post‐trauma interventions.Methods We administered the PCL‐C and SCL‐90 to screen for PTSD and other psychological problems among students who survived the disaster and continued their school studies.The surveys were carried out 3,6,9,and 12 months after the earthquake.A bivariate 2‐level logistic model was used to explore the different levels of sensitivity among students.The factors influencing the relationships between PTSD and depression,and between PTSD and anxiety were examined.Results We analyzed data from 1 677 students,revealing that female students in higher grades were more likely to exhibit symptoms of depression,rather than PTSD,compared with the control group (males in lower grades),and the difference was significant (P<0.05).In contrast,ethnic minorities were more likely to exhibit PTSD symptoms compared to the others.In addition,female students were more likely to exhibit symptoms of anxiety than PTSD.Other effects that did not reach statistical significance were suggested to have a similar influence on PTSD,depression,and anxiety.Conclusion After a natural disaster,specific aspects of depression and anxiety should be examined,avoiding an overemphasis on PTSD in social psychological crisis interventions. | HOU Feng Su LI Ting LI Juan HU Xiao Qin LIU Zhi Yue YUAN Ping | 2011 | Biomedical and Environmental Sciences2011，24，6： | 11 |
| 18 | Casticin induces caspase-mediated apoptosis via activation of mitochondrial pathway and upregulation of DR5 in human lung cancer cells显示文摘Objective:To assess if casticin induces caspase-mediated apoptosis via activation of mitochondrial pathway and upregulation of DR5 in human lung cancer ceils.Methods:Human non-small-cell lung carcinoma cell lines H460,AS49 and H157 were cultured in vitro.The cytotoxic activities were determined using MTT assay.The apoptotic cells death was examined by flow cytometry using PI staining and DMA agarose gel electrophoresis.The activities of caspase-3, -8 and -9 were measured via ELISA.Cellular fractionation was determined by flow cytometry to assess release of cytochrome c and the mitochondrial transmembrane potential.Bcl-2/Bcl-XL/ XIAP/Bid/ DR5 and DR4 proteins were analyzed using western blot.Results:The concentrations required for a 50%decrease in cell growth(IC50) ranged from 1.8 to 3.2 Jt M.Casticin induced rapid apoptosis and triggered a series of effects associated with apoptosis by way of mitochondrial pathway,including the depolarization of the mitochondrial membrane,release of cytochrome c from mitochondria,activation of procaspase-9 and -3,and increase of DNA fragments.Moreover, the pan caspase inhibitor zVAD-FMK and the caspase-3 inhibitor zOEVD-FMK suppressed casticin-induced apoptosis.In addition,casticin induced XIAP and Bcl-XL down-regulation, Bax upregulation and Bid clearage.In H157 cell line,casticin increased expression of DRS at protein levels but not affect the expression of DR4.The prelreatmenl with DR5/Fc chimera protein effectively attenuated casticin-induced apoptosis in H157 cells.No correlation was found between cell sensitivity to casticin and that to p53 status,suggesting that casticin induce a p53- independent apoptosis.Conclusions:Our results demonstrate that casticin induces caspase-mediated apoptosis via activation of mitochondrial pathway and upregulation of DRS in human lung cancer cells. | Yuan Zhou Yi Peng Qi-Qi Mao Xia Li Ming-Wu Chen Ting Su Li Tian Nai-Quan Mao Ling-Zhi Long Mei-Fang Quan Fei Liu Su-Fang Zhou Yong-Xiang Zhao | 2013 | Asian Pacific Journal of Tropical Medicine2013，6，5： | 11 |
| 19 | Prevalence, genetic diversity, and antimicrobial susceptibility profiles of Staphylococcus aureus isolated from bovine mastitis in Zhejiang Province, China显示文摘This study was conducted to determine genetic diversity and antimicrobial susceptibility profiles of Staphylococcus aureus recovered from bovine mastitis in Zhejiang Province, China. Out of 3178 quarter milk samples from 846 lactating cows, among which 459 cows (54.3%) were found HMT positive, 890 quarters (28%) were found having subclinical mastitis. From 75 representative S. aureus isolates, 16 distinct types were identified by pulsed-field gel electrophoresis (PFGE). Four major PFGE types (A, B, C, and D) accounted for 82.7% of all isolates, and type A (41.3%) was observed in multiple herds across the studied areas. Each region was found to have a predominant type: Hangzhou type A (64.1%), Ningbo type C (34.5%) and type B (23.1%), Jinhua type D (53.3%), and Taizhou type C (62.5%). Results of antimicrobial susceptibility tests showed that 90.7% of the isolates were resistant to at least one antimicrobial. Resistance to penicillin and ampicillin (77.3%), tetracycline (60.0%), or erythromycin (48.0%) was observed. The bacteria resistant to multiple antibiotics such as penicillin, ampicillin, tetracycline, and erythromycin were commonly found. The information obtained from this study is useful for designing specific control programs for bovine S. aureus mastitis in this region. | Jian-ping LI Hai-jian ZHOU Lin YUAN Ting HE Song-hua HU | 2009 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2009，10，10： | 11 |
| 20 | Rupintrivir is a promising candidate for treating severe cases of Enterovirus-71 infection显示文摘AIM:To evaluate the suitability of rupintrivir against Enterovirus 71(EV71)induced severe clinical symptoms using computational methods. METHODS:The structure of EV71 3C protease was predicted by homology modeling.The binding free energies between rupintrivir and EV71 3C and human rhinovirus 3C protease were computed by molecular dynamics and molecular mechanics Poisson-Boltzmann/ surface area and molecular mechanics generalized-born/ surface area methods.EV71 3C fragments obtained from clinical samples collected during May to July 2008 in Shanghai were amplified by reverse-transcription and polymerase chain reaction and sequenced. RESULTS:We observed that rupintrivir had favorable binding affinity with EV71 3C protease(-10.76 kcal/mol). The variability of the 3C protein sequence in isolates of various outbreaks,including those obtained in our hospital from May to July 2008,were also analyzed to validate the conservation of the drug binding pocket. CONCLUSION:Rupintrivir,whose safety profiles had been proved,is an attractive candidate and can be quickly utilized for treating severe EV71 infection. | Zhang, Xiao-Nan Song, Zhi-Gang Jiang, Ting Shi, Bi-Sheng Hu, Yun-Wen Yuan, Zheng-Hong | 2010 | World Journal of Gastroenterology2010，16，2： | 10 |
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