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| 1 | Pyrolysis transformation of ZIF-8 wrapped with polytriazine to nitrogen enriched core-shell polyhedrons carbon for supercapacitor显示文摘This work presents a simple effective strategy to synthesize N-doped and shell-controlled carbon nanocages through a package baking approach.A green approach to synthesize core-shell ZIF-8@PTZ nanoparticles involves zinc contained ZIF-8 core wrapped by a N-enriched polytriazine(PTZ).Synthesized core-shell ZIF-8@PTZ nanoparticles are calcinated to further sublime zinc through PTZ shell and washed by HC1,leaving a porous carbon structure.At the meantime,hollow cavities were introduced into N-doped carbon polyhedrons via the sacrifice of ZIF-8 template(noted as ZIF-8@C/N-x).The electrochemical performance of the ZIF-8@C/N-x as supercapacitor electrode has demonstrated high energy density and specific capacitance,as well as a long-term cycleability showing 92%capacitance retention after 10000 cycles.There is a systematic correlation between micro-/meso-porosity of ZIF-8@C/N-x and their electrochemical performances. | Nuoya Wang Xinhua Huang Lei Zhang Jinsong Hu Yimin Chao Ruikun Zhao | 2021 | Frontiers of Chemical Science and Engineering2021，15，4： | 1 |
| 2 | Novel attitude determination method by integration of electronic level meter,INS,and low-cost turntable for level attitude evaluation and calibration of INS显示文摘Attitude references are greatly needed for the evaluation and calibration of Inertial Navigation Systems(INSs),which are widely used in gravimeter,marine,and aeronautical navigation.High-accuracy turntable,INS,and Global Navigation Satellite System have been utilized to verify the performance of relatively low-accuracy INS.The accuracy requirement of the attitude reference continuously increases with the rapid improvement of inertial sensors and navigation algorithms.However,the cost of attitude determination system increases rapidly with the increase of attitude accuracy requirement.To solve this limitation,the integration of level meter,INS,and low-cost turntable is proposed to provide level attitude,such as roll and pitch.The turntable is utilized to rotate the INS.An integration model of the level meter and INS is built to estimate the level attitude and reduce the cost of the turntable.The proposed method successfully avoids the dependence on high-accuracy turntables.An observability degree analysis is conducted to improve the level atti-tude accuracy further.The simulation and turntable test results indicate that the proposed method can provide high-accuracy level attitude without high-accuracy INS or turntable and is applicable to error calibration and attitude evaluation of INS. | Anlan YANG Peida HU Genming LIU Rong ZHANG Qiuping WU Ruikun ZHOU | 2023 | Chinese Journal of Aeronautics2023，36，4： | 0 |
| 3 | Characterization of the viral fibroblast growth factor homolog of Helicoverpa armigera single nucleopolyhedrovirus显示文摘Fibroblast growth factor(FGF) is found throughout multicellular organisms; however, fgf homologs(vfgf) have only been identified among viruses in lepidopteran baculoviruses. The function of v FGFs from Group I alphabaculoviruses, including Autographa californica multiple nucleopolyhedrovirus(Ac MNPV) and Bombyx mori nucleopolyhedrovirus(Bm NPV), involves accelerated killing of infected larvae by both viruses. The v FGF of Group II alphabaculovirus is structurally different from that of Group I alphabaculovirus, with a larger C-terminal region and additional N-linked glycosylation sites. In this study, we characterized the Group II alphabaculovirus v FGF of Helicoverpa armigera single nucleopolyhedrovirus(Hear NPV). The transcription and expression of vfgf was detected at 3 h and 16 h post-infection in Hear NPV-infected cells. To further study v FGF function, we constructed vfgf-knockout and-repaired Hear NPV bacmids and investigated their affect in both cultured cells and insects. Deletion of vfgf had no effect on budded-virus production or viral DNA replication in cultured Hz AM1 cells. However, bioassays showed that Hear NPV vfgf deletion significantly increased the median lethal dose and delayed the median lethal time by ~12 h in the host insect when the virus was delivered orally. These results suggested that v FGF is an important virulent factor for HearN PV infection and propagation in vivo. | Feifei Yin Ruikun Du Wenhua Kuang Guang Yang Hualin Wang Fei Deng Zhihong Hu Manli Wang | 2016 | Virologica Sinica2016，31，3： | 0 |
| 4 | Cyclin A2/cyclin-dependent kinase 1-dependent phosphorylation of Top2a is required for S phase entry during retinal development in zebrafish显示文摘Cyclin-dependent kinase 1 (CDK1) plays an essential role in cell cycle regulation.However,as mouse Cdk1embryos die early,the role of CDK1 in regulating the cell cycle and embryo development remains unclear.Here,we showed that zebrafish cdk1^(-/-)embryos exhibit severe microphthalmia accompanied by multiple defects in S phase entry,M phase progression,and cell differentiation but not in interkinetic nuclear migration.We identified Top2a as a potential downstream target and cyclin A2 and cyclin B1 as partners of Cdk1 in cell cycle regulation via an in silico analysis.While depletion of either cyclin A2 or Top2a led to the decreased S phase entry in zebrafish retinal cells,the depletion of cyclin B1 led to M phase arrest.Moreover,phosphorylation of Top2a at serine 1213 (S1213) was nearly abolished in both cdk1 and ccna2mutants,but not in ccnb1 mutants.Furthermore,overexpression of TOP2A^(S1213D),the phosphomimetic form of human TOP2A,rescued S phase entry and alleviated the microphthalmia defects in both cdk1^(-/-)and ccna2^(-/-)embryos.Taken together,our data suggest that Cdk1 interacts with cyclin A2 to regulate S phase entry partially through Top2a phosphorylation and interacts with cyclin B1 to regulate M phase progression. | Miaomiao Jin Jingyu Li Ruikun Hu Baijie Xu Guanliang Huang Weilai Huang Bo Chen Jie He Ying Cao | 2021 | Journal of Genetics and Genomics2021，48，1： | 0 |
| 5 | An in vitro recombination-based reverse genetic system for rapid mutagenesis of structural genes of the Japanese encephalitis virus显示文摘Japanese encephalitis virus(JEV) is one of the most common pathogens of severe viral encephalitis, which is a severe threat to human health. Despite instability of the JEV genome in bacteria, many strategies have been developed to establish molecular clone systems of JEV, providing convenient tools for studying the virus life cycle and virus–host interactions. In this study, we adapted an In-Fusion enzyme-based in vitro recombination method to construct a reverse genetic system of JEV, thereby providing a rapid approach to introduce mutations into the structural genes. A truncated genome without the structural genes was constructed as the backbone, and the complementary segment containing the structural genes was recombined in vitro, which was then transfected directly into virus-permissive cells. The progeny of the infectious virus was successfully detected in the supernatant of the transfected cells, and showed an identical phenotype to its parental virus. To provide a proof-of-principle, the 12 conserved cysteine residues in the envelope(E) protein of JEV were respectively mutated using this approach, and all mutations resulted in a complete failure to generate infectious virus. However, a leucine-tophenylanine mutation at amino acid 107 of the E protein did not interfere with the production of the infectious virus. These results suggested that all 12 cysteines in the E protein are essential for the JEV life cycle. In summary, a novel reverse genetic system of JEV was established for rapidly introducing mutations into structural genes, which will serve as a useful tool for functional studies. | Ruikun Du Manli Wang Zhihong Hu Hualin Wang Fei Deng | 2015 | Virologica Sinica2015，30，5： | 0 |
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