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| 1 | Characterization of the essential role of bone morphogenetic protein 9 (BMP9) in osteogenic differentiation of mesenchymal stem cells (MSCs) through RNA interference显示文摘Mesenchymal stem cells(MSCs)are multipotent stem cells and capable of differentiating into multiple cell types including osteoblastic,chondrogenic and adipogenic lineages.We previously identified BMP9 as one of the most potent BMPs that induce osteoblastic differentiation of MSCs although exact molecular mechanism through which BMP9 regulates osteogenic differentiation remains to be fully understood.Here,we seek to develop a recombinant adenovirus system to optimally silence mouse BMP9 and then characterize the important role of BMP9 in osteogenic differentiation of MSCs.Using two different siRNA bioinformatic prediction programs,we design five siRNAs targeting mouse BMP9(or simB9),which are expressed under the control of the converging H1 and U6 promoters in recombinant adenovirus vectors.We demonstrate that two of the five siRNAs,simB9-4 and simB9-7,exhibit the highest efficiency on silencing exogenous mouse BMP9 in MSCs.Furthermore,simB9-4 and simB9-7 act synergistically in inhibiting BMP9-induced expression of osteogenic markers,matrix mineralization and ectopic bone formation from MSCs.Thus,our findings demonstrate the important role of BMP9 in osteogenic differentiation of MSCs.The characterized simB9 siRNAs may be used as an important tool to investigate the molecular mechanism behind BMP9 osteogenic signaling.Our results also indicate that recombinant adenovirus-mediated expression of siRNAs is efficient and sustained,and thus may be used as an effective delivery vehicle of siRNA therapeutics. | Shujuan Yan Ruyi Zhang Ke Wu Jing Cui Shifeng Huang Xiaojuan Ji Liping An Chengfu Yuan Cheng Gong Linghuan Zhang Wei Liu Yixiao Feng Bo Zhang Zhengyu Dai Yi Shen Xi Wang Wenping Luo Bo Liu Rex C.Haydon Michael J.Lee Russell R.Reid Jennifer Moriatis Wolf Qiong Shi Hue H.Luu Tong-Chuan He Yaguang Weng | 2018 | Genes & Diseases2018，5，2： | 8 |
| 2 | The development of a sensitive fluorescent protein-based transcript reporter for high throughput screening of negative modulators of lncRNAs显示文摘While the human genome is pervasively transcribed,<2%of the human genome is transcribed into protein-coding mRNAs,leaving most of the transcripts as noncoding RNAs,such as microRNAs and long-noncoding RNAs(lncRNAs),which are critical components of epigenetic regulation.lncRNAs are emerging as critical regulators of gene expression and genomic stability.However,it remains largely unknown about how lncRNAs are regulated.Here,we develop a highly sensitive and dynamic reporter that allows us to identify and/or monitor negative modulators of lncRNA transcript levels in a high throughput fashion.Specifically,we engineer a fluorescent fusion protein by fusing three copies of the PEST destruction domain of mouse ornithine decarboxylase(MODC)to the C-terminal end of the codon-optimized bilirubin-inducible fluorescent protein,designated as dBiFP,and show that the dBiFP protein is highly destabilized,compared with the commonly-used eGFP protein.We further demonstrate that the dBiFP signal is effectively down-regulated when the dBiFP and mouse lncRNA H19 chimeric transcript is silenced by mouse H19-specific siRNAs.Therefore,our results strongly suggest that the dBiFP fusion protein may serve as a sensitive and dynamic transcript reporter to monitor the inhibition of lncRNAs by microRNAs,synthetic regulatory RNA molecules,RNA binding proteins,and/or small molecule inhibitors so that novel and efficacious inhibitors targeting the epigenetic circuit can be discovered to treat human diseases such as cancer and other chronic disorders. | Zongyue Zeng Bo Huang Shifeng Huang Ruyi Zhang Shujuan Yan Xinyi Yu Yi Shu Chen Zhao Jiayan Lei Wenwen Zhang Chao Yang Ke Wu Ying Wu Liping An Xiaojuan Ji Cheng Gong Chengfu Yuan Linghuan Zhang Wei Liu Yixiao Feng Bo Zhang Zhengyu Dai Yi Shen Xi Wang Wenping Luo Rex C.Haydon Hue H.Luu Lan Zhou Russell R.Reid Tong-Chuan He Xingye Wu | 2018 | Genes & Diseases2018，5，1： | 4 |
| 3 | Laser acupuncture for mild to moderate depression in a primary care setting-a randomised controlled trial显示文摘 | Quah-Smith JI Tang WM Russell J | 2005 | Acupunct Med2005，23，3： | 1 |
| 4 | Rethinking phonons:The issue of disorder显示文摘Current understanding of phonons treats them as plane waves/quasi-particles of atomic vibration that propagate and scatter.The problem is that conceptually,when any level of disorder is introduced,whether compositional or structural,the character of vibrational modes in solids changes,yet nearly all theoretical treatments continue to assume phonons are still waves.For example,the phonon contributions to alloy thermal conductivity(TC)rely on this assumption and are most often computed from the virtual crystal approximation(VCA).Good agreement is obtained in some cases,but there are many instances where it fails—both quantitatively and qualitatively.Here,we show that the conventional theory and understanding of phonons requires revision,because the critical assumption that all phonons/normal modes resemble plane waves with well-defined velocities is no longer valid when disorder is introduced.Here we show,surprisingly,that the character of phonons changes dramatically within the first few percent of impurity concentration,beyond which phonons more closely resemble the modes found in amorphous materials.We then utilize a different theory that can treat modes with any character and experimentally confirm its new insights. | Hamid Reza Seyf Luke Yates Thomas L.Bougher Samuel Graham Baratunde A.Cola Theeradetch Detchprohm Mi-Hee Ji Jeomoh Kim Russell Dupuis Wei Lv Asegun Henry | 2017 | npj Computational Materials2017，，1： | 1 |
| 5 | Preparation of nanosize Pt clusters using ion exchange of Pt(NH3) 4 2+ inside mesoporous channel of MCM-41显示文摘 | Ryong Ryoo Chang Hyun Ko Ji Man Kim Russell Howe | 1996 | Catalysis Letters (-)1996，，1： | 1 |
| 6 | Practical application of the PAR index:an illustrative comparison of the outcome of treatment using two fixed appliance techniques显示文摘 | Buchanan IB Russell JI Clark JD | 1996 | Br J Orthod1996，23，： | 1 |
| 7 | The use of transvaginal ultrasound to aspirate bilateral hydrosalpinges prior to in vitro fertilization : a case report显示文摘 | Russell JB Rodriguez Z Komins JI | 1991 | J In Vitro Fert Embryo Transf1991，8，4： | 1 |
| 8 | 离心式压缩机在阻塞状态下的运行研究显示文摘离心式压缩机偶尔会有在阻塞区或其附近运行的情况。通过建立阻塞状态下所允许的各种操作极限,在测试数据和模拟数据的基础上对离心式压缩机在阻塞区的运行状态进行了研究。考虑到机芯性能、轴向载荷、蜗壳特性以及径向载荷的变化。讨论了叶轮叶片的激振问题。对多级压缩机和双段压缩机在流量明显高于设计流量运行时,叶轮不匹配所造成的影响给出了特别的建议。压缩机过载运行范围的限制不仅影响操作灵活性,还要求配备更复杂的控制系统。讨论了离心式压缩机在阻塞状态下运行的气动、结构以及转子动力学的问题,提出了离心式压缩机在阻塞区可使用和不可使用的基本特性。 | Rainer Kurz Russell K Marechale Edward J Fowler Min Ji Michael J Cave | 2017 | 天然气与石油2017，35，1： | 1 |
| 9 | Practical application of the PAR index: An illustrative comparison of the outcome of treatment using two fixed appliance techniques显示文摘 | Buchanan IB Russell JI Clark JD | 1996 | Br J Orthod1996，23，4： | 1 |
| 10 | Molecular mediators of Porphyromonas gingivalis-induced T-cell apoptosis显示文摘 | Harris JI Russell RR Curtis MA | 2002 | Oral Microbiol Immunol2002，17，4： | 1 |
| 11 | Folic acid use and major congenital malformations in offspring of women with epilepsy: a prospective study from the UK Epilepsy and Pregnancy Register 显示文摘 | Morrow JI Hunt S J Russell AJ | 2009 | J Neurol Neurosurg Psychiatry2009，80，： | 1 |
| 12 | Primary nursing : An evaluation of its effects on patient Perception of care and staff Sactisfaction显示文摘 | Sellick K J Russell Beckmann JI | 2003 | In- tennational Nursing Studies2003，40，5： | 1 |
| 13 | Practical application of the PAR index:an illustrative comparison of the outcome of treatment using two fixed appliance techniques显示文摘 | Buchanan IB Russell JI Clark JD | 1996 | Br J Orthod1996，23，4： | 1 |
| 14 | Molecular characterization of radiation- and chemically induced mutations associated with neuromuscular tremors, runting, juvenile lethality, and sperm defects in jdf2 mice显示文摘 | Mitchell Walkowicz Yonggang Ji Xiaojia Ren Bernhard Horsthemke Liane B. Russell Dabney Johnson Eugene M. Rinchik Robert D. Nicholls Lisa Stubbs | 1999 | Mammalian Genome1999，，9： | 1 |
| 15 | A 3-year oral health dose-response study of sodium monofluorophosphate dentifrices with and without zinc citrate:anti-caries results显示文摘 | Stephen KW Greatmor SL Russell JI | 1988 | Community Dent Oral Epidemiol1988，16，： | 1 |
| 16 | Incorporation of bispohospho nates into adenine nucleotides by amoebae of the cellular slime mold dictyostelium discoideum显示文摘 | Rogers MJ Ji X Russell RGG | 1994 | Biochem J1994，303，1： | 1 |
| 17 | Influence of the electronic structure on the ductile behavior of B2 CsCl-type AB intermetallics 显示文摘 | GSCITNEIDNER K A Jr JI M WANG C Z HO K M RUSSELL A M MUDRYK Y A BECKER A T LARSON J L | 2009 | Acta Materialia2009，57，19： | 1 |
| 18 | Qualititave of Aspects of Pear Flavour显示文摘 | LF Russell HA Quamme JI GRAY | 1981 | Journal of Food Science1981，，： | 1 |
| 19 | Practical applicationof the PAR index ; an illustrative comparison of the out-come of treatment using two fixed appliance techniques显示文摘 | Buchanan IB Russell JI Clark JD | 1996 | Br J Orthod1996，23，4： | 1 |
| 20 | Dkk1 -mediated inhibition of Wnt signaling in bone results in osteopenia显示文摘 | Ji Li Ildiko Sarosi Russell C. Cattley James Pretorius Frank Asuncion Mario Grisanti Sean Morony Stephen Adamu Zhaopo Geng Wanrong Qiu Paul Kostenuik David L. Lacey W. Scott Simonet Brad Bolon Xueming Qian Victoria Shalhoub Michael S. Ominsky Hua Zhu Ke X | 2006 | Bone2006，，4： | 1 |
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