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| 1 | The Genome of Artemisia annua Provides Insight into the Evolution of Asteraceae Family and Artemisinin Biosynthesis显示文摘Artemisia annua,通常已知的同样香甜的苦恼或 Qinghao,是到中国的一个灌木土著人并且长被用于药用的目的。A。annua 现在作为有势力抗疟药混合物的唯一的生来的来源全球性被栽培, artemisinin。这里,我们报导 A 的 1.74-gigabase 染色体的一个高质量的草稿集会。annua,高度异质接合,富于重复定序,并且包含 63 ? 226 编码蛋白质的基因,在定序的植物种类之中的最大的数字之一。我们发现了那,作为在 Asteraceae 的一些定序的染色体之一, A。annua 染色体包含对这大被子植物 clade 特定的很多基因。尤其是,扩大和编码涉及萜烯生合成的酶的基因的功能的多样化与 artemisinin biosynthetic 小径的进化一致。我们进一步由介绍那 A 的 transcriptome 揭示了。annua 发展了复杂 transcriptional 规章的网络位于 \O 下面 artemisinin 生合成。把转基因的 A 基于我们产生了的全面 genomic 和 transcriptomic 分析。artemisinin 高级的生产的 annua 线,它现在为大规模生产准备好了并且将从而帮助遇见增加 artemisinin 的全球需求的挑战。 | Qian Shen Lida Zhang Zhihua Liao Shengyue Wang Tingxiang Yan Pu Shi Meng Liu Xueqing Fu Qifang Pan Yuliang Wang Zongyou Lv Xu Lu Fangyuan Zhang Weimin Jiang Yanan Ma Minghui Chen Xiaolong Hao Ling Li Yueli Tang Gang Lv Yan Zhou Xiaofen Sun Peter E. Brodelius Jocelyn K.C. Rose Kexuan Tang | 2018 | Molecular Plant2018，11，6： | 47 |
| 2 | Application of next-generation sequencing technology to precision medicine in cancer: joint consensus of the Tumor Biomarker Committee of the Chinese Society of Clinical Oncology显示文摘Next-generation sequencing(NGS) technology is capable of sequencing millions or billions of DNA molecules simultaneously.Therefore, it represents a promising tool for the analysis of molecular targets for the initial diagnosis of disease, monitoring of disease progression, and identifying the mechanism of drug resistance. On behalf of the Tumor Biomarker Committee of the Chinese Society of Clinical Oncology(CSCO) and the China Actionable Genome Consortium(CAGC), the present expert group hereby proposes advisory guidelines on clinical applications of NGS technology for the analysis of cancer driver genes for precision cancer therapy. This group comprises an assembly of laboratory cancer geneticists, clinical oncologists, bioinformaticians,pathologists, and other professionals. After multiple rounds of discussions and revisions, the expert group has reached a preliminary consensus on the need of NGS in clinical diagnosis, its regulation, and compliance standards in clinical sample collection. Moreover, it has prepared NGS criteria, the sequencing standard operation procedure(SOP), data analysis, report, and NGS platform certification and validation. | Xuchao Zhang Zhiyong Liang Shengyue Wang Shun Lu Yong Song Ying Cheng Jianming Ying Weiping Liu Yingyong Hou Yangqiu Li Yi Liu Jun Hou Xiufeng Liu Jianyong Shao Yanhong Tai Zheng Wang Li Fu Hui Li Xiaojun Zhou Hua Bai Mengzhao Wang You Lu Jinji Yang Wenzhao Zhong Qing Zhou Xuening Yang Jie Wang Cheng Huang Xiaoqing Liu Xiaoyan Zhou Shirong Zhang Hongxia Tian Yu Chen Ruibao Ren Ning Liao Chunyan Wu Zhongzheng Zhu Hongming Pan Yanhong Gu Liwei Wang Yunpeng Liu Suzhan Zhang Tianshu Liu Gong Chen Zhimin Shao Binghe Xu Qingyuan Zhang Ruihua Xu Lin Shen Yilong Wu | 2019 | Cancer Biology & Medicine2019，16，1： | 14 |
| 3 | Geospatial Service Web:towards integrated cyberinfrastructure for GIScience显示文摘A geospatial cyberinfrastructure is needed to support advanced GIScience research and education activities.However,the heterogeneous and distributed nature of geospatial resources creates enormous obstacles for building a unified and interoperable geospatial cyberinfrastructure.In this paper,we propose the Geospatial Service Web(GSW)to underpin the development of a future geospatial cyberinfrastructure.The GSW excels over the traditional spatial data infrastructure by providing a highly intelligent geospatial middleware to integrate various geospatial resources through the Internet based on interoperable Web service technologies.The development of the GSW focuses on the establishment of a platform where data,information,and knowledge can be shared and exchanged in an interoperable manner.Theoretically,we describe the conceptual framework and research challenges for GSW,and then introduce our recent research toward building a GSW.A research agenda for building a GSW is also presented in the paper. | GONG Jianya WU Huayi ZHANG Tong GUI Zhipeng LI Zhenlong YOU Lan SHEN Shengyu ZHENG Jie GENG Jing QI Kunlun YANG Wenjing LI Zhenqiang YU Jingmin | 2012 | Geo-Spatial Information Science2012，15，2： | 5 |
| 4 | Single-cell atlas reveals a distinct immune profile fostered by T cell-B cell crosstalk in triple negative breast cancer显示文摘Background:Characterizing the unique immune microenvironment of each tumor is of great importance for better predicting prognosis and guiding cancer immunotherapy.However,the unique features of the immune microenvironment of triple negative breast cancer(TNBC)compared with other subtypes of breast cancer remain elusive.Therefore,we aimed to depict and compare the immune landscape among TNBC,human epidermal growth factor receptor 2-positive(HER2^(+))breast cancer,and luminal-like breast cancer.Methods:Single-cell RNA sequencing(scRNA-seq)was performed on CD45^(+)immune cells isolated from human normal breast tissues and primary breast tumors of various subtypes.By analyzing the scRNA-seq data,immune cell clusters were identified and their proportions as well as transcriptome features were compared among TNBC,human HER2^(+)breast cancer,and luminal-like breast cancer.Pseudotime and cell-cell communication analyses were also conducted to characterize the immune microenvironment.Results:ScRNA-seq data of 117,958 immune cells were obtained and 31 immune clusters were identified.A unique immunosuppressive microenvironment in TNBC was decoded as compared to that in HER2^(+)or luminal-like breast cancer,which was characterized by higher proportions of regulatory T cells(Tregs)and exhausted CD8+T cells and accompanied by more abundant plasma cells.Tregs and exhausted CD8+T cells in TNBC exhibited increased immunosuppression signature and dysfunctional scores.Pseudotime analyses showed that B cells tended to differentiate to plasma cells in TNBC.Cell-cell communication analyses indicated that these unique features are fostered by the diversified T cell-B cell crosstalk in TNBC.Based on the T cell-B cell crosstalk,a prognostic signaturewas established that could effectively predict the prognosis status for patients with TNBC.Additionally,it was found that TNBC had a higher proportion of cytotoxic natural killer(NK)cells,whereas HER2^(+)or luminal-like breast cancer lost this feature,suggesting thatHER2^(+)or luminal-like breast cancer,but not TNBC,may benefit from NK-based immunotherapy.Conclusions:This study identified a distinct immune feature fostered by T cell-B cell crosstalk in TNBC,which provides better prognostic information and effective therapeutic targets for breast cancer. | Shuning Ding Niu Qiao Qingchen Zhu Yiwei Tong Shengyue Wang Xiaosong Chen Qiang Tian Yichuan Xiao Kunwei Shen | 2023 | Cancer Communications2023，43，6： | 2 |
| 5 | Transfer of β-hydroxy-β-methylbutyrate from sows to their offspring and its impact on muscle fiber type transformation and performance in pigs显示文摘Background: Previous studies suggested that supplementation of lactating sows with β-hydroxy-β-methylbutyrate(HMB) could improve the performance of weaning pigs, but there were little information in the muscle fiber type transformation of the offspring and the subsequent performance in pigs from weaning through finishing in response to maternal HMB consumption. The purpose of this study was to determine the effect of supplementing lactating sows with HMB on skeletal muscle fiber type transformation and growth of the offspring during d 28 and180 after birth. A total of 20 sows according to their body weight were divided into the control(CON, n = 10) or HMB groups(HMB, n = 10). Sows in the HMB group were supplemented with β-hydroxy-β-methylbutyrate calcium(HMB-Ca) 2 g/kg feed during d 1 to 27 of lactation. After weaning, 48 mixed sex piglets were blocked by sow treatment and fed standard diets for post-weaning, growing, finishing periods. Growth performance was recorded during d 28 to 180 after birth. Pigs were slaughtered on d 28(n = 6/treatment) and 180(n = 6/treatment) postnatal and the longissimus dorsi(LD) was collected, respectively.Results: The HMB-fed sows during lactation showed increased HMB concentration(P < 0.05) in milk and LD of weaning piglets(P < 0.05). In addition, offsprings in HMB group had a higher finishing BW and lean percentage than did pigs in CON group(P < 0.05), meanwhile, compared with pigs from sows fed the CON diet, pigs from sows fed HMB diet showed higher type Ⅱ muscle fiber cross-sectional area(CSA), elevated myosin heavy chain(MyHC) Ⅱb and Sox6 mRNA, and fast-MyHC protein levels in LD(P < 0.05).Conclusions: HMB supplemented to sow diets throughout lactation increases the levels of HMB in maternal milk and skeletal muscle of pigs during d 28 after birth and promotes subsequent performance of pigs between d 28 and 180 of age by enhancing glycolytic muscle fiber transformation. | Haifeng Wan Jiatao Zhu Caimei Wu Pan Zhou Yong Shen Yan Lin Shengyu Xu Lianqiang Che Bin Feng Jian Li Zhengfeng Fang De Wu | 2017 | Journal of Animal Science and Biotechnology2017，8，2： | 2 |
| 6 | Anti-adhesive effects of diverse self-assemhled monolayers in nanoimprint lithography显示文摘 | Wu Chihwei Shen Yungkang Chuang Shengyu | 2007 | Sensors and Actuators A2007，139，： | 1 |
| 7 | AN ANT COLONY ALGORITHM FOR MINIMUM UNSATISFIABLE CORE EXTRACTION显示文摘Explaining the causes of infeasibility of Boolean formulas has many practical applications in electronic design automation and formal verification of hardware.Furthermore,a minimum explanation of infeasibility that excludes all irrelevant information is generally of interest.A smallest-cardinality unsatisfiable subset called a minimum unsatisfiable core can provide a succinct explanation of infea-sibility and is valuable for applications.However,little attention has been concentrated on extraction of minimum unsatisfiable core.In this paper,the relationship between maximal satisfiability and mini-mum unsatisfiability is presented and proved,then an efficient ant colony algorithm is proposed to derive an exact or nearly exact minimum unsatisfiable core based on the relationship.Finally,ex-perimental results on practical benchmarks compared with the best known approach are reported,and the results show that the ant colony algorithm strongly outperforms the best previous algorithm. | Zhang Jianmin Shen Shengyu Li Sikun | 2008 | Journal of Electronics(China)2008，25，5： | 1 |
| 8 | Mechanism of pore pressure variation in multiple coal reservoirs,western Guizhou region,South China显示文摘Pore pressure is an important parameter in coalbed methane(CBM)exploration and development;however,the distribution pattern and mechanism for pore pressure differences in the Upper Permian CBM reservoirs are poorly understood in the western Guizhou region of South China.In this study,lateral and vertical variations and mechanisms for pore pressure differences are analyzed based on 126 injection-falloff and in-situ stress well test data measured in Permian coal reservoirs.Generally,based on the pore pressure gradient and coefficient in coal reservoirs of the western Guizhou region,five zones can be delineated laterally:the mining areas of Zhina,northem Liupanshui,northern Guizhou,northwestern Guizhou and southern Liupanshui.Vertically,there are two main typical patterns:i)the pore pressure gradient(or coefficient)is nearly unchanged in different coal reservoirs,and ii)the pore pressure gradient(or coefficient)has cyclic variations in a borehole profile with multiple coal seams,which suggests the existence of a'superimposed CBM system'.The mechanism analysis indicates that coal permeability,thermal evolution stage and hydrocarbon generation contribute little to pore pressure differences in coal reservoirs in the western Guizhou region.The present-day in-situ stress field,basement structure and tectonic activity may be the dominant factors affecting lateral pore pressure differences.The sealing capacity of caprocks and the present-day in-situ stress field are significant para-meters causing vertical pore pressure differences in coal reservoirs.These results are expected to provide new geological references for CBM exploration and develop-ment in the western Guizhou region. | Wei JU Zhaobiao YANG Yulin SHEN Hui YANG Geoff WANG Xiaoli ZHANG Shengyu WANG | 2021 | Frontiers of Earth Science2021，15，4： | 0 |
| 9 | Development of digital organ-on-a-chip to assess hepatotoxicity and extracellular vesicle-based anti-liver cancer immunotherapy显示文摘Organ-on-a-chip systems have been increasingly recognized as attractive platforms to assess toxicity and to develop new therapeutic agents.However,current organ-on-a-chip platforms are limited by a“single pot”design,which inevitably requires holistic analysis and limits parallel processing.Here,we developed a digital organ-on-a-chip by combining a microwell array with cellular microspheres,which significantly increased the parallelism over traditional organ-on-a-chip for drug development.Up to 127 uniform liver cancer microspheres in this digital organ-on-a-chip format served as individual analytical units,allowing for analysis with high consistency and quick response.Our platform displayed evident anti-cancer efficacy at a concentration of 10μM for sorafenib,and had greater alignment than the“single pot”organ-on-a-chip with a previous in vivo study.In addition,this digital organ-on-a-chip demonstrated the treatment efficacy of natural killer cell-derived extracellular vesicles for liver cancer at 50μg/mL.The successful development of this digital organ-on-a-chip platform provides high-parallelism and a low-variability analytical tool for toxicity assessment and the exploration of new anticancer modalities,thereby accelerating the joint endeavor to combat cancer. | Guohua Wu Jianguo Wu Zihan Li Shengyu Shi Di Wu Xuanbo Wang Han Xu Hui Liu Yixiao Huang Rending Wang Jia Shen Zhihong Dong Shuqi Wang | 2022 | Bio-Design and Manufacturing2022，5，3： | 0 |
| 10 | Shell-formation mediated surface composition of uniform two-component microparticles fabricated by micro-fluidic spray drying: Effect of component size and solubility显示文摘This work is aimed to study the effects of component size and solubility on the surface composition of spray dried(SD)uniform two-component particles fabricated by micro-fluidic spray dryer.Various precursor liquid consisting of small molecular of methionine(Met,33 g/L)or lysine(Lys,739 g/L)and large-sized silica(12 nm)were prepared by adjusting the mass ratio of components.X-ray energy disper-sive results showed that the respective enrichment degree(De)of Met and Lys on the surface of SD-M1S9 and-L1S9 prepared at 150 C were 182±9% and 125±14%.The De of hydrophobic Met for SD-M1S1 and-M9S1 were 46±9% and 4±2%,respectively,whereas relative hydrophilic Lys mainly distributed internal of the particle meanwhile the De of silica on the surface for SD-L1S1 and-L9S1 were 17±4%and 12±1%,respectively.Drying temperature(120 and 180℃)showed more apparent effect on the De of amino acid for the particles of less amino acid.The possible formation mechanism of surface composition and the surface composition impact on the wettability of particles were explored.These results provide new guidance for manufacturing functional SD powders with various components. | Xiaoqian Ma Shen Yan Shengyu Zhang Quanyi Yin Xiaodong Chen Winston Duo Wu | 2022 | Particuology2022，20，8： | 0 |
| 11 | A workfow to Predict the Present-day in-situ Stress Field in Tectonically Stable Regions显示文摘Knowledge of the present-day in-situ stress distribution is greatly import-ant for better understanding of conventional and unconventional hydro-carbon reservoirs in many aspects,e.g,reservoir management,wellbore stability asssment,etc.In tectonically stable regions,the present-day in-situ stress field in terms of stress distribution is 1argely controlled by lithological changes,which can be predicted through|a numerical simulation method incorporating specific mechanical properties of the subsurface reservoir.In this study,a workflow was presented to predict the present-day in-situ stress field based on the finite element method(FEM).Sequentially,it consists of:i)building a three-dimensional(3D)geometric framework,i)creating a 3D petrophysical parameter field,11)integrating the geometric framework with petrophysical parameters,iv)setting up a 3D heterogeneous geomechanical model,and finally,v)calculating the present-day in-situ stress distribution and calibrating the prediction with measured stress data,e.g.,results from the extended leak-off tests(XLOTs).The approach was sucessfully applied to the Block W in Ordos Basin of central China.The results indicated that the workflow and models presented in this study could be used as an effective tool to provide insights into stress perturbations in subsurface reservoirs and geological references for subsequent analysis. | Wei Ju Ke Xu Jian Shen Chao Li Guozhang Li Haoran Xu Shengyu Wang | 2019 | Journal of Environmental & Earth Sciences2019，1，2： | 0 |
| 12 | Artificial duplicate reads in sequencing data of 454 Genome Sequencer FLX System显示文摘454 染色体音序器(GS ) FLX 系统是展示由的下一代的定序系统之一长读,高精确性,并且超离频产量。基于乳剂 PCR 的机制,一个唯一的 DNA 模板将仅仅产生在在 GS 上被放大并且定序 FLX 以后读的一个唯一的序列。然而,偏导 DNA 模板的扩大可能发生在乳剂 PCR 的过程,它导致人工的副本的生产读。在每个 DNA 模板对另外一个唯一的条件下面, 3.49%?18.14% 总数在数据被发现人工的 GS 定序 FLX 读副本读。这些副本读可以导致定序数据的误解,特殊注意应该对他们介绍给数据的潜在的偏爱被给予。 | Hui Dong Yangyi Chen Yan Shen Shengyue Wang Guoping Zhao Weirong Jin | 2011 | Acta Biochimica et Biophysica Sinica2011，43，6： | 0 |
| 13 | Crystallization Kinetics of Nanophase Glass-Ceramics as Magnetic Disk Substrate显示文摘基于 SiO_2-Al_2O_3-Li_2O-K_2O-MgO-ZnO 的系统作为一个主要水晶阶段包含贝它石英的眼镜陶艺为更高的存储能力为申请被调查到磁性的存储底层。父母眼镜在为 3 约 4 h 的某些温度被准备,然后起核心作用并且结晶。结晶化动力学 ofglass 陶艺也被学习。谷物尺寸被 Scherrer 公式和传播电子显微镜学(TEM ) 的图象处理估计。结果证明 Avrami 代表决心是 3.88 ,活化能 189.3 +- 7 kJ/mol 和谷物缩放 30 约 60 nm.A 眼镜陶艺,包括的谷物分发和形态学的详细微观结构ofnano水晶被 TEM 描绘,X光检查衍射( XRD ),微分扫描热量计( DSC ),微分热分析( DTA )。在微观结构和机械性质之间的关系也被讨论。 | Shengyu JIANG Jiancheng ZHANG Feng GU Yue SHEN Hua WANG | 2006 | Journal of Materials Science & Technology2006，22，2： | 0 |
| 14 | Designing TiO_(2)/Au/Prussian blue heterostructures nanorod arrays for ultra-stable cycle and ultra-fast response electrochromism显示文摘Prussian blue(PB)is an anodic coloring candidate in the wide area of electrochromic(EC)applications.However,the co-influence of weak adhesion and low electrical conductivity leads to the poor stability and slow switching speed.To tackle this bottleneck,a novel TiO_(2)/Au/PB nanorod array is designed through hydrothermal and electrodeposition approaches on fluorine-doped tin oxide(FTO)glass.Such a designed ternary array structure could not only increase reactive site and conductivity,but also improve ion storage capacity and promote charge transfer,attributed to the synergistic effect of TiO_(2)/Au/PB core–shell heterostructure and the localized surface plasmon resonance(LSPR)effect of Au nanoparticles.Besides,density functional theory(DFT)calculation confirms the strong interaction between rutile TiO_(2)and FTO substrate,which contributes to the improvement of EC cycle stability.Benefiting from these effects,the TiO_(2)/Au/PB film shows a fast coloration/bleaching response of 1.08/2.01 s(2.17/4.48 s,PB film)and ultra-stable EC performance of 86.8%after 20,000 cycles(50%after 600 cycles,PB film).Furthermore,the high-intensity light source can be shot clearly by the designed and assembled EC iris device(ECID)with TiO_(2)/Au/PB film as an EC layer,while the photograph without an ECID is blurry,confirming the feasibility of the material in portable digital products. | Miao Xu Kang Li Shen Wang Shengyu Zhou Hulin Zhang Hongbo Xu Jiupeng Zhao Yao Li | 2023 | Nano Research2023，16，2： | 0 |
| 15 | Environmental hypoxia induces apoptosis in large yellow croaker Larimichthys crocea via both intrinsic and extrinsic pathways显示文摘Hypoxia has become an unfavorable factor affecting the sustainable development of the large yellow croaker Larimichthys crocea,an economically important mariculture fish in China.Apoptosis is a consequence of hypoxia on fish.However,the effects of hypoxia stress on apoptosis in L.crocea remain largely unknown.We investigated the effect of environmental hypoxia on apoptosis in L.crocea.Results show that hypoxia induced apoptosis in L.crocea both in vivo and in vitro.The mitochondrial membrane potential was significantly reduced in large yellow croaker fry(LYCF)cells.The expression levels of Bcell lymphoma/leukemia-2(Bcl-2)m RNA and protein were also significantly decreased in the liver and LYCF cells during 96 h and 48 h of hypoxia stress,respectively,whereas the expression level of Bcl-2 associated X(Bax)mRNA,Casp3 mRNA,and activity of caspase-3/7/9 were significantly increased,indicating that hypoxia induced caspase-dependent intrinsic apoptosis in L.crocea.The expression level of the apoptosis-inducing factor(AIF)protein was significantly increased in the liver and LYCF cells.The level of AIF protein was significantly decreased in the cytoplasm but increased in the nuclei of L.crocea,demonstrating that hypoxia induced the AIF-mediated caspase-independent intrinsic apoptosis.In addition,the activity of caspase-8 was significantly increased,indicating that hypoxia stress induced extrinsic apoptosis in L.crocea.Therefore,hypoxia induced apoptosis in L.crocea through both the intrinsic and extrinsic pathways.The present study accumulated basic biological information to help elucidate the mechanism of hypoxia response in marine fish. | Shengyu LUO Cheng LIU Xinming GAO Jingqian WANG Yibo ZHANG Jie DING Congcong HOU Junquan ZHU Bao LOU Weiliang SHEN Xiongfei WU Chundan ZHANG | 2023 | Journal of Oceanology and Limnology2023，41，6： | 0 |
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