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| 1 | Physical Location of the Rice Pi-5(t), Glh and RTSV Genes by ISH of BAC Clones显示文摘Mapplng of low of slngle-copy sequences on plant chromosomes has proven dirricult because ofvery low frequency or slgnal detectlon. Rlce BAC Ilbrary ls belng used wldely ln rlce genome research due tolts distlnctlve advantages over other llbrary systems. In tkls study, two blotln-Iabeled rice BAC clones closelylinked to a rlce blast reslstance, green Ieaf hopper reslstance and tungro spherlcal virus resistance gene,Pi-5(t),Glh, RTSV, were in situ hybrldlred to rlce ckromosomes. They were located on the long arm andshort arm of ckromosome 4 with FL value of 40% and 100% respectively-The rrequency of signal detectionreacked 46. 8% and 59- 2%- The slgnal location were conslstent wltk the selectlve marker on rice saturatedm0Iecular map. The results demonstrated the advautages to Iocate BAC cIones to ckromosomes by in sho hybrldization and will racilitate tke rlce low of slngle-copy gene Iocation by using the BAC library. | Yan Huimin, Song Yunchun, Li Lijia, Bi Xuezhi, Fu Binying(College of Life Sciences, Wuhan University, Wuhan 430072, China) | 1998 | Wuhan University Journal of Natural Sciences1998，3，2： | 24 |
| 2 | Physical location of rice Gm-6,Pi-5(t) genes in O.officinalis with BAC-FISH显示文摘A fluorescence in situ hybridization (FISH) procedure was adopted to physically map two rice BAC clones 24E21 and 4F22 linked to Gm-6 and Pi-5(t) in O. offi-cinalis. FISH results showed that the two BAC clones were located at 4L. The percentage distance from the centromere to the hybridization sites was 72 ± 2.62 for 24E21 and 54± 5.43 for 4F22, the detection rates were 52.70% and 61.2%. The results obtained from the BAC and plasmid clones, RG214 and RZ565 of cultivated rice and O. officinalis were the same. This suggested that the markers, RG214 and RZ565 of cultivated rice and O. officinalis were in the same BAC clones. The homologous sequences of Gm-6 and Pi-5(t) in O. officinalis were positions that signals existed on the 4L. Many signals were observed when no Cot-1 DNA blocked. This also showed that repetitive sequences were some ho-molgous between cultivated rice and O. officinalis. The identification of chromosome 4 of O. officinalis is based on Jena et al. (1994). In our study, we discussed | QIN Rui WEI Wenhui JIN Weiwei HE Guangcun NING Shunbin YU Shunwu SONG Yunchun | 2001 | Chinese Science Bulletin2001，46，8： | 6 |
| 3 | Comparative genome research between maize and rice using genomic in situ hybridization显示文摘Using the genomic DNAs of maize and rice as probes respectively, the homology of maize and rice genomes was assessed by genomic in situ hybridization. When rice genomic DNAs were hybridized to maize, all chromosomes displayed many multiple discrete regions, while each rice chromosome delineated a single consecutive chromosomal region after they were hybridized with maize genomic DNAs. The results indicate that the genomes of maize and rice share high homology, and confirm the proposal that maize and rice are diverged from a common ancestor. | Shunbin Ning Weiwei Jin Ling Wang Yi Ding Yunchun Song | 2001 | Chinese Science Bulletin2001，46，8： | 5 |
| 4 | Logging Evaluation of the Ordovician Carbonate Reservoir Beds in the Lungudong Region,Tarim Basin显示文摘在最近的年里,大进步在 Tarim 盆的 Lungudong 区域在油和煤气的探索常常被取得了。然而,进步在它的主要生产油的地平线的评估是慢的奥陶纪碳酸盐水库床。基于以前的研究并且在象钻,地质学和油测试那样的各种各样的数据上,在有各单个井的成像和常规记载数据的解释的联合,并且通过分析和比较,在成像并且在这个区域的为碳酸盐水库床的四种类型的常规记载的鉴定方法在这篇论文被总结。为四个水库床参数的计算公式,我。e。在这个区域的页岩内容,孔,渗透和油浸透被建议;并且在这个区域的水库床被划分成三个层次(我, II 和 III ) 由联合油测试数据并且记载数据,水库床的有效的孔的更低的限制和有效水库的破裂孔,床分别地作为 1.8% 和 0.04% 被决定。物理性质参数被常规记载的曲线计算,并且为水库开发的最有益的区域包括地被预言。在飞机上,水库床参数的高值的地区主要在 N-S-trending 罢工滑倒差错被集中, Sangtamu 差错 horst 地区并且接近 LG38 很好区域;垂直地, Yijianfang 形成的水库床参数比 Yingshan 和 Lianglitage 形成的那些好。 | YANG Wenjing XIAO Chengwen LIU Luofu QI Guangzhong JIANG Zhenxue YUAN Yunchun WANG Weili YANG song | 2010 | Acta Geologica Sinica(English Edition)2010，84，5： | 5 |
| 5 | Determination of copy number for 5S rDNA and centromeric sequence RCS2 in rice by Fiber-FISH显示文摘The copy number of 5S rDNA and centromerie sequence RCS2 was determined by extended DNA fiber based fluorescence in situ hybridization (Fiber-FISH) in rice (Oryza sativa ssp. indica cv. Guangluai No. 4) genome. In order to determine the copy number, it is necessary to know the basepair number that a given length DNA fiber contains under a microscope. Therefore, the length of two DNA frag-ments, in which the basepair number had been already known, was measured. The insert sequence of the tested BAC 38D17 was 136 kb and its extended DNA was 56.4 μm long, 2.41 kb/μm on average, while that of the tested BAC 44B4 was 144.5 kb in total and 55.7 μm long, 2.60 kb/μm on average under the microscope. They were very close to the theoretical value of B-DNA in the Watson-Crick DNA model, which is 2.97 kb/μm. According to the average value of basepair number per μm of the two samples mentioned above, that is, 2.51 kb/μm, it could be estimated that the copy number was about 686 for 5S rDNA and 286-1121 for the | Zongyun Li Siluo Huang Weiwei Jin Shunbin Ning Yunchun Song Lijia Li | 2002 | Chinese Science Bulletin2002，47，3： | 5 |
| 6 | A novel method for in situ detection of apoptotic cell death in plants显示文摘A simple novel protocol suitable for in situ detection of apoptotic plant cell death is presented. This protocol combines the chromosome spreading technique with an extended in situ end labeling version for plants and makes it possible to detect apoptosis directly at chromosome,nuclear and DNA levels simultaneously with high-sensitivity. False positive and nonspecific signals can be efficiently avoided. Moreover, the changes of chromosomes, nuclei and DNA during the process of apoptosis can be characterized in situ. To illustrate this method, salt stress-induced cell death has been investigated in maize root meristematic cells. | Shunbin Ning Yunchun Song Ling Wang Lihua Liu | 1999 | Chinese Science Bulletin1999，44，11： | 4 |
| 7 | Mouse Karyotype Obtained by Combining DAPI Staining with Image Analysis显示文摘In this study, mitotic metaphase chromosomes in mouse were identified by a new chromosome fluorescence banding technique combining DAPI staining with image analysis. Clear 4’, 6-diamidino-2-phenylindole (DAPI) multiple bands like G-bands could be produced in mouse. The MetaMorph software was then used to generate linescans of pixel intensity for the banded chromosomes from short arm to long arm. These linescans were sufficient not only to identify each individual chromosome but also analyze the physical sites of bands in chromosome. Based on the results, the clear and accurate karyotype of mouse metaphase chromosomes was established. The technique is therefore considered to be a new method for cytological studies of mouse. | DAI Xiaohua YANG Guangxu LIU Jingyu SONG Yunchun | 2006 | Wuhan University Journal of Natural Sciences2006，11，2： | 3 |
| 8 | Introgression of perennial teosinte genome into maize and identification of genomic in situ hybridization and microsatellite markers显示文摘 | Qilin Tang Tingzhao Rong Yunchun Song | 2005 | Crop Science2005，45，： | 1 |
| 9 | Blend Films of Chitosan/Starch显示文摘BlendFilmsofChitosan/StarchDuYumin,XiaZuyong,LuRongDepartmentofEnvironmentalScience,WuhanUniversity,Wuhan430072,ChinaAbstract... | Song Yunchun Ren Nan Mao Ninghui Liu Lihua | 1997 | Wuhan University Journal of Natural Sciences1997，2，2： | 1 |
| 10 | Strong convergence of iteration algorithms for a countable family of nonexpansive mappings显示文摘 | SONG Yisheng ZHENG Yunchun | 2009 | Nonlinear Analysis:Theory Methods & Application2009，71，7： | 1 |
| 11 | FISH analysis of the integration patterns in transgenic rice co-transformed by micro-projectile bombardment显示文摘Using multi-color fluorescence in situ hybridization (FISH), we localized transferred barnase-psl and pHctinG DNA sequences onto chromosomes of two trans-genie rice plants, named Q12 and Q13, both of which were produced by micro-projectile bombardment. In both Q12 and Q13, each detected cell showed 2-3 signal spots on their chromosomes respectively. The signals of both barnase-psl and pHctinG were mostly detected in the adjacent chromosomal sites in which their signals were overlapped and could be recognized by the signal color on the metaphase chromosomes. Fiber FISH further demonstrated that the multiple copies in each of the two DNA sequences distributed adjacently on the DNA fiber in Q13. Combined with the results of Southern hybridization, the possible integration patterns in transgenic rice co-transformed by micro-projectile bombardment have been discussed. | JIN Weiwei LI Zongyun NING Shunbin LING Dinghou LI Lijia SONG Yunchun | 2001 | Chinese Science Bulletin2001，46，23： | 1 |
| 12 | Introgressionof perennial teosinte genome into maize and identification ofgenomic in situ hybridization and microsatellite markers显示文摘 | Qiling Tang Tingzhao Rong Yunchun Song | 2005 | CropScience2005，45，： | 1 |
| 13 | Genetic diversity in oil and vegetable mustard (Brassica juncea) landraces revealed by SRAP markers显示文摘 | Xiao-ming Wu Bi-yun Chen Guangyuan Lu Han-zhong Wang Kun Xu Gao Guizhan Yunchun Song | 2009 | Genetic Resources and Crop Evolution2009，，7： | 1 |
| 14 | Adaptive watermark generation mechanism based on time series prediction for stream processing显示文摘The data stream processing framework processes the stream data based on event-time to ensure that the request can be responded to in real-time.In reality,streaming data usually arrives out-of-order due to factors such as network delay.The data stream processing framework commonly adopts the watermark mechanism to address the data disorderedness.Watermark is a special kind of data inserted into the data stream with a timestamp,which helps the framework to decide whether the data received is late and thus be discarded.Traditional watermark generation strategies are periodic;they cannot dynamically adjust the watermark distribution to balance the responsiveness and accuracy.This paper proposes an adaptive watermark generation mechanism based on the time series prediction model to address the above limitation.This mechanism dynamically adjusts the frequency and timing of watermark distribution using the disordered data ratio and other lateness properties of the data stream to improve the system responsiveness while ensuring acceptable result accuracy.We implement the proposed mechanism on top of Flink and evaluate it with realworld datasets.The experiment results show that our mechanism is superior to the existing watermark distribution strategies in terms of both system responsiveness and result accuracy. | Yang SONG Yunchun LI Hailong YANG Jun XU Zerong LUAN Wei LI | 2021 | Frontiers of Computer Science2021，15，6： | 0 |
| 15 | Physical Location of Terminal Markers Belonging to Five Linkage Groups in Maize RFLP Map Using in Situ Hybridization显示文摘PhysicalLocationofTerminalMarkersBelongingtoFiveLinkageGroupsinMaizeRFLPMapUsinginSituHybridizationMaoNinghui,SongYunchun,L... | Mao Ninghui Song Yunchun Liu Lihua Hang Chao Yan Chunhong | 1997 | Wuhan University Journal of Natural Sciences1997，2，4： | 0 |
| 16 | A correlation method of detecting and estimating interactions of QTLs显示文摘More and more studies demonstrate that a great deal of interactions among the quantitative trait loci (QTLs) are far more than those detected by single markers. A correlation method was proposed for estimating the interactions of multiple QTLs detected by multi-markers in several mapping populations. Genetic implication of this method and usage were discussed. | HU Zhongli SUN Qixin ZHANG Xiufu SONG Yunchun ZHANG Qifa | 2002 | Chinese Science Bulletin2002，47，8： | 0 |
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