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| 1 | The Reference Genome of Tea Plant and Resequencing of 81 Diverse Accessions Provide Insights into Its Genome Evolution and Adaptation显示文摘Tea plant is an important economic crop,which is used to produce the world's oldest and most widely consumed tea beverages.Here,we present a high-quality reference genome assembly of the tea plant(Camellia sinensis var.sinensis)consisting of 15 pseudo-chromosomes.LTR retrotransposons(LTR-RTs)account for 70.38%of the genome,and we present evidence that LTR-RTS play critical roles in genome size expansion and the transcriptional diversification of tea plant genes through preferential insertion in promoter regions and introns.Genes,particularly those coding for terpene biosynthesis pro-teins,associated with tea aroma and stress resistance were significantly amplified through recent tandem duplications and exist as gene clusters in tea plant genome.Phylogenetic analysis of the sequences of 81 tea plant accessions with diverse origins revealed three well-differentiated tea plant populations,support-ing the proposition for the southwest origin of the Chinese cultivated tea plant and its later spread to western Asia through introduction.Domestication and modern breeding left significant signatures on hundreds of genes in the tea plant genome,particularly those associated with tea quality and stress resis-tance.The genomic sequences of the reported reference and resequenced tea plant accessions provide valuable resources for future functional genomics study and molecular breeding of improved cul-tivars of tea plants. | Enhua Xia Wei Tong Yan Hou Yanlin An Linbo Chen Qiong Wu Yunlong Liu Jie Yu Fangdong Li Ruopei Li Penghui Li Huijuan Zhao Ruoheng Ge Jin Huang Ali Inayat Mallano Yanrui Zhang Shengrui Liu Weiwei Deng Chuankui Song Zhaoliang Zhang Jian Zhao Shu Wei Zhengzhu Zhang Tao Xia Chaoling Wei Xiaochun Wan | 2020 | Molecular Plant2020，13，7： | 51 |
| 2 | A 4-bp Insertion at ZmPLA 1 Encoding a Putative Phospholipase A Generates Haploid Induction in Maize显示文摘 | Chenxu Liu Xiang Li Dexuan Meng Yu Zhong Chen Chen Xin Dong Xiaowei Xu Baojian Chen Wei Li Liang Li Xiaolong Tian Haiming Zhao Weibin Song Haishan Luo Qinghua Zhang Jinsheng Lai Weiwei Jin Jianbing Yan Shaojiang Chen | 2017 | Molecular Plant2017，10，3： | 39 |
| 3 | Laparoscopic versus open pancreaticoduodenectomy for pancreatic ductal adenocarcinoma:a propensity score matching analysis显示文摘Background:A growing body of evidence supports the use of laparoscopic pancreaticoduodenectomy(LPD)as an efficient and feasible surgical technique.However,few studies have investigated its applicability in pancreatic ductal adenocarcinoma(PDAC),and the long-term efficacy of LPD on PDAC remains unclear.This study aimed to compare the short-and long-term outcomes between LPD and open pancreaticoduodenectomy(OPD)for PDAC.Methods:The data of patients who had OPD or LPD for PDAC between January 2013 and September 2017 were retrieved.Their postoperative outcomes and survival were compared after propensity score matching.Results:A total of 309 patients were included.After a 2:1 matching,93 cases in the OPD group and 55 in the LPD group were identified.Delayed gastric emptying(DGE),particularly grade B/C DGE,occurred less frequently in the LPD group than in the OPD group(1.8%vs.36.6%,P<0.001;1.8%vs.22.6%,P=0.001).The overall complication rates were significantly lower in the LPD group than in the OPD group(49.1%vs.71.0%,P=0.008),whereas the rates of major complications were similar(10.9%vs.14.0%,P=0.590).In addition,the median overall survival was comparable between the two groups(20.0 vs.18.7 months,P=0.293).Conclusion:LPD was found to be technically feasible with efficacy similar to OPD for patients with PDAC. | Wentao Zhou Weiwei Jin Dansong Wang Chao Lu Xuefeng Xu Renchao Zhang Tiantao Kuang Yucheng Zhou Wenchuan Wu Dayong Jin Yiping Mou Wenhui Lou | 2019 | Cancer Communications2019，39，1： | 14 |
| 4 | De novo mutation in ATP6V1B2 impairs lysosome acidification and causes dominant deafness-onychodystrophy syndrome显示文摘 | Yongyi Yuan Jianguo Zhang Qing Chang Jin Zeng Feng Xin Jianjun Wang Qingyan Zhu Jing Wu Jingqiao Lu Weiwei Guo Xukun Yan Hui Jiang Binfei Zhou Qi Li Xue Gao Huijun Yuan Shiming Yang Dongyi Han Zixu Mao Ping Chen Xi Lin Pu Dai | 2014 | Cell Research2014，24，11： | 13 |
| 5 | Insight-HXMT observations of the first binary neutron star merger GW170817显示文摘Finding the electromagnetic(EM) counterpart of binary compact star merger, especially the binary neutron star(BNS) merger,is critically important for gravitational wave(GW) astronomy, cosmology and fundamental physics. On Aug. 17, 2017,Advanced LIGO and Fermi/GBM independently triggered the first BNS merger, GW170817, and its high energy EM counterpart,GRB 170817 A, respectively, resulting in a global observation campaign covering gamma-ray, X-ray, UV, optical, IR, radio as well as neutrinos. The High Energy X-ray telescope(HE) onboard Insight-HXMT(Hard X-ray Modulation Telescope) is the unique high-energy gamma-ray telescope that monitored the entire GW localization area and especially the optical counterpart(SSS17 a/AT2017 gfo) with very large collection area(~1000 cm^2) and microsecond time resolution in 0.2-5 MeV. In addition,Insight-HXMT quickly implemented a Target of Opportunity(ToO) observation to scan the GW localization area for potential X-ray emission from the GW source. Although Insight-HXMT did not detect any significant high energy(0.2-5 MeV) radiation from GW170817, its observation helped to confirm the unexpected weak and soft nature of GRB 170817 A. Meanwhile,Insight-HXMT/HE provides one of the most stringent constraints(~10^(-7) to 10^(-6) erg/cm^2/s) for both GRB170817 A and any other possible precursor or extended emissions in 0.2-5 MeV, which help us to better understand the properties of EM radiation from this BNS merger. Therefore the observation of Insight-HXMT constitutes an important chapter in the full context of multi-wavelength and multi-messenger observation of this historical GW event. | TiPei Li ShaoLin Xiong ShuangNan Zhang FangJun Lu LiMing Song XueLei Cao Zhi Chang Gang Chen Li Chen TianXiang Chen Yong Chen YiBao Chen YuPeng Chen Wei Cui WeiWei Cui JingKang Deng YongWei Dong YuanYuan Du MinXue Fu GuanHua Gao He Gao Min Gao MingYu Ge YuDong Gu Ju Guan ChengCheng Guo DaWei Han Wei Hu Yue Huang Jia Huo ShuMei Jia LuHua Jiang WeiChun Jiang Jing Jin YongJie Jin Bing Li ChengKui Li Gang Li MaoShun Li Wei Li Xian Li XiaoBo Li XuFang Li YanGuo Li ZiJian Li ZhengWei Li XiaoHua Liang JinYuan Liao CongZhan Liu GuoQing Liu HongWei Liu ShaoZhen Liu XiaoJing Liu Yuan Liu YiNong Liu Bo Lu XueFeng Lu Tao Luo Xiang Ma Bin Meng Yi Nang JianYin Nie Ge OU JinLu Qu Na Sai Liang Sun Yin Tan Lian Tao WenHui Tao YouLi Tuo GuoFeng Wang HuanYu Wang Juan Wang WenShuai Wang YuSa Wang XiangYang Wen BoBing WU Mei Wu GuangCheng Xiao He Xu YuPeng Xu LinLi Yan JiaWei Yang Sheng Yang YanJi Yang AiMei Zhang ChunLei Zhang ChengMo Zhang Fan Zhang HongMei Zhang Juan Zhang Qiang Zhang Shu Zhang Tong Zhang Wei Zhang WanChang Zhang WenZhao Zhang Yi Zhang Yue Zhang YiFei Zhang YongJie Zhang Zhao Zhang ZiLiang Zhang HaiSheng Zhao JianLing Zhao XiaoFan Zhao ShiJie Zheng Yue Zhu YuXuan Zhu ChangLin Zou | 2018 | Science China(Physics,Mechanics & Astronomy)2018，61，3： | 12 |
| 6 | Normal Structure and Function of Endothecium Chloroplasts Maintained by ZmMs33-Mediated Lipid Biosynthesis in Tapetal Cells Are Critical for Anther Development in Maize显示文摘Genic male sterility(GMS)is critical for heterosis utilization and hybrid seed production.Although GMS mutants and genes have been studied extensively in plants,it has remained unclear whether chloroplast-associated photosynthetic and metabolic activities are involved in the regulation of anther development.In this study,we characterized the function of ZmMs33/ZmGPAT6,which encodes a member of the glycerol-3-phosphate acyltransferase(GPAT)family that catalyzes the first step of the glycerolipid synthetic pathway.We found that normal structure and function of endothecium(En)chloroplasts maintained by ZmMs33-mediated lipid biosynthesis in tapetal cells are crucial for maize anther development.ZmMs33 is expressed mainly in the tapetum at early anther developmental stages and critical for cell proliferation and expansion at late stages.Chloroplasts in En cells of wild-type anthers function as starch storage sites before stage 10 but as photosynthetic factories since stage 10 to enable starch metabolism and carbohydrate supply.Loss of ZmMs33 function inhibits the biosynthesis of glycolipids and phospholipids,which are major components of En chloroplast membranes,and disrupts the development and function of En chloroplasts,resulting in the formation of abnormal En chloroplasts containing numerous starch granules.Further analyses reveal that starch synthesis during the day and starch degradation at night are greatly suppressed in the mutant anthers,leading to carbon starvation and low energy status,as evidenced by low trehalose-6-phosphate content and a reduced ATP/AMP ratio.The energy sensor and inducer of autophagy,SnRK1,was activated to induce early and excessive autophagy,premature PCD,and metabolic reprogramming in tapetal cells,finally arresting the elongation and development of mutant anthers.Taken together,our results not only show that ZmMs33 is required for normal structure and function of En chloroplasts but also reveal that starch metabolism and photosynthetic activities of En chloroplasts at different developmental stages are essential for normal anther development.These findings provide novel insights for understanding how lipid biosynthesis in the tapetum,the structure and function of En chloroplasts,and energy and substance metabolism are coordinated to maintain maize anther development. | Taotao Zhu Ziwen Li Xueli An Yan Long Xiaofeng Xue Ke Xie Biao Ma Danfeng Zhang Yijian Guan Canfang Niu Zhenying Dong Quancan Hou Lina Zhao Suowei Wu Jinping Li Weiwei Jin Xiangyuan Wan | 2020 | Molecular Plant2020，13，11： | 9 |
| 7 | Genome-Wide Analysis of Histone Modifications: H3K4me2, H3K4me3, H3K9ac, and H3K27ac in Oryza sativa L. Japonica显示文摘当以前的研究证明了 histone 修正能由调整基因抄写影响工厂生长和开发时,在这些修正和基因表示之间的关系的知识仍然是有限的。这研究使用了高产量的定序跟随的染色质 immunoprecipitation (ChIP-Seq ) ,到调查四 histone 修正的染色体宽的分发:H3K4 (H3K4me2 和 H3K4me3 ) 和在 Oryza sativa L 的 H3K9 和 H3K27 (H3K9ac 和 H3K27ac ) 的 acylation 的 di 和 trimethylation。装饰用的梨树。由分析出版 DNase-Seq 数据,这研究探索了 DNase 过分敏感(DH ) 沿着米饭染色体的地点。histone 标记主要在通用区域出现了并且在基因的抄写开始地点(TSS ) 附近被充实。这分析证明四 histone 修正和 DH 地点都与活跃抄写被联系。而且,四 histone 修正与答应被用来在米饭基因注解预言失踪的基因的特征的抄本 regionsa 是高度并发的。预言被试验性地证实二的抄写预言了失踪的基因进一步验证。而且,顺序主题分析被构造以便识别 DH 地点和许多通常认为的抄写因素绑定地点。 | Zhou Du Hui Li Qiang Wei Xin Zhao Chunchao Wang Qilin Zhu Xin Yi Wenying Xua X. Shirley Liu Weiwei Jin Zhen Su | 2013 | Molecular Plant2013，6，5： | 8 |
| 8 | Global cultivated land mapping at 30 m spatial resolution显示文摘Cultivated land is one of the most important types of land cover in the global mapping of land cover, and its variation influences economic development, food security, and ecological environment protection. Existing products of global cultivated land mapping have a low resolution, and high spatial resolution products are in demand. This study uses global remote sensing image datasets in 2000/2010 with a spatial resolution of 30 m(Landsat TM/ETM+, HJ-1), MODIS 250 m NDVI time-serial data, and many types of reference data. An three-layer extraction method based on pixels, objects, and knowledge(POK) was adopted to ease cultivated land extraction in global-scale 30 m images, i.e., cultivated land classification based on pixel-scale multi-feature optimization, cultivated land automatic identification based on objects, and interactive object processing based on information service and priori knowledge. Global 30 m cultivated land mapping was accomplished for the two reference years(2000 and 2010), and statistical analysis was conducted on the data. Results showed that the total cultivated land area was 1.903 billion ha and 1.960 billion ha, respectively. Accuracy assessments showed that overall accuracy of global cultivated land mapping are higher than 92% for both the two reference years. The global cultivated land products in 2000/2010 developed in this research are superior to their international counterparts in terms of spatial resolution and classification accuracy. They also provide significant basic data on global food security, ecological environment supervision, and global change. | CAO Xin CHEN XueHong ZHANG WeiWei LIAO AnPing CHEN LiJun CHEN ZhiGang CHEN Jin | 2016 | Science China(Information Sciences)2016，59，12： | 8 |
| 9 | Allele-defined genome reveals biallelic differentiation during cassava evolution显示文摘Dear Editor,Generation of heterozygous genomes by hybridization between or within species can help maintain plant diversity and serve as a potential source of new species(Baek et al.,2018).Moreover,genomic heterozygosity is associated with genomic coadaptation,developmental stability,and heterosis.Accurate definition of alleles in haplotypes is necessary to precisely characterize allelic variation controlling agriculturally important traits(Shi et al.,2019).Currently,most released genomes have mosaic assembly of haplotypes due to random selection or collapse of alleles during genome assembly(Shi et al.,2019),which masked allelic variation and functional differentiation of divergent alleles in heterozygous species. | Wei Hu Changmian Ji Haitao shi Zhe Liang Zehong Ding Jianqiu Ye Wenjun Ou Gang Zhou Weiwei Tie Yan Yan Jinghao Yang Xiaoying Yang Yunxie Wei Zhiqiang Jin Jianghui Xie Ming Peng Wenquan Wang Anping Guo Biyu Xu Jianchun Guo Songbi Chen Liming Ma Mingcheng Wang Yang Zhou Xiaolong Li Ruoxi Li Siyi Guo Xinhui Xiao Zhongqing Wan Feifei An Jie Zhang Qingyun Leng Yin Li Ray Ming Kaimian Li | 2021 | Molecular Plant2021，14，6： | 8 |
| 10 | Terahertz detectors based on superconducting hot electron bolometers显示文摘Low noise terahertz(THz)heterodyne detectors based on superconducting niobium nitride(NbN) hot electron bolometers(HEBs)have been studied.The HEB consists of a planar antenna and an NbN bridge connecting across the antenna's inner terminals on a high-resistivity Si substrate.The double sideband noise temperatures at 4.2 K without corrections have been characterized from 0.65 to 3.1 THz.The excess quantum noise factor β of about 4 has been obtained,which agrees well with the calculated value.Allan variance of the HEB has been measured,and Allan time up to 20 s is obtained using a microwave feedback method.Also,the intermediate frequency gain bandwidth(GBW)was measured using two dierent methods,resulting in same GBW value of about 3.5 GHz. | JIANG Yi JIN BiaoBing XU WeiWei KANG Lin CHEN Jian WU PeiHeng | 2012 | Science China(Information Sciences)2012，55，1： | 8 |
| 11 | Zircon SHRIMP U–Pb age of Late Jurassic OIB-type volcanic rocks from the Tethyan Himalaya: constraints on the initial activity time of the Kerguelen mantle plume显示文摘This work presents zircon U–Pb age and wholerock geochemical data for the volcanic rocks from the Lakang Formation in the southeastern Tethyan Himalaya and represents the initial activity of the Kerguelen mantle plume. SHRIMP U–Pb dating of zircons from the volcanic rocks yielded a ^(206) Pb/^(238) U age of 147 ± 2 Ma that reflects the time of Late Jurassic magmatism. Whole rock analyses of major and trace elements show that the volcanic rocks are characterized by high content of Ti O_2(2.62 wt%–4.25 wt%) and P_2O_5(0.38 wt%–0.68 wt%), highly fractionated in LREE/HREE [(La/Yb)N= 5.35–8.31] with no obvious anomaly of Eu, and HFSE enrichment with no obvious anomaly of Nb and Ta, which are similar to those of ocean island basalts and tholeiitic basaltic andesites indicating a mantle plume origin. The Kerguelen mantle plume produced a massive amount of magmatic rocks from Early Cretaceous to the present, which widely dispersed from their original localities of emplacement due to the changing motions of the Antarctic, Australian, and Indian plates. However, our new geochronological and geochemical results indicate that the Kerguelen mantle plume started from the Late Jurassic. Furthermore, we suggest that the Kerguelen mantle plume may played a significant role in the breakup of eastern Gondwanaland according to the available geochronological, geochemical and paleomagnetic data. | Yuruo Shi Chenyang Hou J. Lawford Anderson Tianshui Yang Yiming Ma Weiwei Bian Jingjie Jin | 2018 | Acta Geochimica2018，37，3： | 6 |
| 12 | A temperature-activated nanocomposite metamaterial absorber with a wide tunability显示文摘新奇薄、灵活的宽带电磁的微波吸收器与 nanocomposites 被认识到并且完成宽频率 tunability (从 10 ~ 17.2 GHz ) 由活跃地调整抵抗。建议吸收器被可伸缩的屏幕打印优化 nanoparticle 墨水到灵活绝缘的合成底层上制作。基于底层的形状记忆效果和 nanocomposite 频率的 piezoresistive 效果选择表面,一个可控制的表电阻,和从而悦耳的波浪吸收性能,能以一种激活温度、动态地稳定的方式被认识到。结果由利用人工的材料和基于聚合物的 nanocomposites 的聪明的行为的以前低估的内在的性质为活跃电磁的设备的设计提供新尺寸。 | Weiwei Li Lingyu Zhao Zhaohe Dai Hao Jin Feng Duan Junchao Liu Zhihui Zeng Jun Zhao Zhong Zhang | 2018 | Nano Research2018，11，7： | 6 |
| 13 | Physical location of rice Gm-6,Pi-5(t) genes in O.officinalis with BAC-FISH显示文摘A fluorescence in situ hybridization (FISH) procedure was adopted to physically map two rice BAC clones 24E21 and 4F22 linked to Gm-6 and Pi-5(t) in O. offi-cinalis. FISH results showed that the two BAC clones were located at 4L. The percentage distance from the centromere to the hybridization sites was 72 ± 2.62 for 24E21 and 54± 5.43 for 4F22, the detection rates were 52.70% and 61.2%. The results obtained from the BAC and plasmid clones, RG214 and RZ565 of cultivated rice and O. officinalis were the same. This suggested that the markers, RG214 and RZ565 of cultivated rice and O. officinalis were in the same BAC clones. The homologous sequences of Gm-6 and Pi-5(t) in O. officinalis were positions that signals existed on the 4L. Many signals were observed when no Cot-1 DNA blocked. This also showed that repetitive sequences were some ho-molgous between cultivated rice and O. officinalis. The identification of chromosome 4 of O. officinalis is based on Jena et al. (1994). In our study, we discussed | QIN Rui WEI Wenhui JIN Weiwei HE Guangcun NING Shunbin YU Shunwu SONG Yunchun | 2001 | Chinese Science Bulletin2001，46，8： | 6 |
| 14 | Genomic, Transcriptomic, and Phenomic Variation Reveals the Complex Adaptation of Modern Maize Breeding显示文摘 | Haijun Liu Xiaqing Wang Marilyn L. Warburton Weiwei Wen Minliang Jin Min Deng Jie Liu Hao Tong Qingchun Pan Xiaohong Yang Jianbing Yan | 2015 | Molecular Plant2015，8，6： | 6 |
| 15 | Copper-ceria sheets catalysts: Effect of copper species on catalytic activity in CO oxidation reaction显示文摘Copper-ceria sheets catalysts with different loadings of copper(2 wt.%, 5 wt.% and 10 wt.%) supported on ceria nanosheets were synthesized via a depositioneprecipitation(DP) method. The prepared catalysts were systematically characterized with various structural and textural detections including X-ray diffraction(XRD), Raman spectra, transmission electron microscopy(TEM), X-ray absorption fine structure(XAFS), and temperature-programmed reduction by hydrogen(H_2-TPR), and tested for the CO oxidation reaction. Notably, the sample containing 5 wt.% of Cu exhibited the best catalytic performance as a result of the highest number of active CuO species on the catalyst surface. Further increase of copper content strongly affects the dispersion of copper and thus leads to the formation of less active bulk CuO phase, which was verified by XRD and H_2-TPR analysis. Moreover, on the basis of in-situ diffuse reflectance infrared Fourier transform spectroscopy(in-situ DRIFTS) results, the surface Cu^+ species, which are derived from the reduction of Cu^(2+), are likely to play a crucial role in the catalyzing CO oxidation.Consequently, the superior catalytic performance of the copper-ceria sheets is mainly attributed to the highly dispersed CuO_x cluster rather than Cu-[O_x]-Ce structure, while the bulk CuO phase is adverse to the catalytic activity of CO oxidation. | Linying Du Weiwei Wang Han Yan Xu Wang Zhao Jin Qisheng Song Rui Si Chunjiang Jia | 2017 | Journal of Rare Earths2017，35，12： | 5 |
| 16 | Auranofin mitigates systemic iron overload and induces ferroptosis via distinct mechanisms显示文摘Iron homeostasis is essential for health;moreover,hepcidin-deficiency results in iron overload in both hereditary hemochromatosis and iron-loading anemia.Here,we identified iron modulators by functionally screening hepcidin agonists using a library of 640 FDA-approved drugs in human hepatic Huh7 cells.We validated the results in C57BL/6J mice and a mouse model of hemochromatosis(Hfe^(−/−)mice).Our screen revealed that the anti-rheumatoid arthritis drug auranofin(AUR)potently upregulates hepcidin expression.Interestingly,we found that canonical signaling pathways that regulate iron,including the Bmp/Smad and IL-6/Jak2/Stat3 pathways,play indispensable roles in mediating AUR’s effects.In addition,AUR induces IL-6 via the NF-κB pathway.In C57BL/6J mice,acute treatment with 5 mg/kg AUR activated hepatic IL-6/hepcidin signaling and decreased serum iron and transferrin saturation.Whereas chronically treating male Hfe^(−/−)mice with 5 mg/kg AUR activated hepatic IL-6/hepcidin signaling,decreasing systemic iron overload,but less effective in females.Further analyses revealed that estrogen reduced the ability of AUR to induce IL-6/hepcidin signaling in Huh7 cells,providing a mechanistic explanation for ineffectiveness of AUR in female Hfe^(−/−)mice.Notably,high-dose AUR(25 mg/kg)induces ferroptosis and causes lipid peroxidation through inhibition of thioredoxin reductase(TXNRD)activity.We demonstrate the ferroptosis inhibitor ferrostatin significantly protects liver toxicity induced by highdose AUR without comprising its beneficial effect on iron metabolism.In conclusion,our findings provide compelling evidence that TXNRD is a key regulator of ferroptosis,and AUR is a novel activator of hepcidin and ferroptosis via distinct mechanisms,suggesting a promising approach for treating hemochromatosis and hepcidin-deficiency related disorders. | Lei Yang Hao Wang Xiang Yang Qian Wu Peng An Xi Jin Weiwei Liu Xin Huang Yuzhu Li Shiyu Yan Shuying Shen Tingbo Liang Junxia Min Fudi Wang | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 5 |
| 17 | Comparative genome research between maize and rice using genomic in situ hybridization显示文摘Using the genomic DNAs of maize and rice as probes respectively, the homology of maize and rice genomes was assessed by genomic in situ hybridization. When rice genomic DNAs were hybridized to maize, all chromosomes displayed many multiple discrete regions, while each rice chromosome delineated a single consecutive chromosomal region after they were hybridized with maize genomic DNAs. The results indicate that the genomes of maize and rice share high homology, and confirm the proposal that maize and rice are diverged from a common ancestor. | Shunbin Ning Weiwei Jin Ling Wang Yi Ding Yunchun Song | 2001 | Chinese Science Bulletin2001，46，8： | 5 |
| 18 | Determination of copy number for 5S rDNA and centromeric sequence RCS2 in rice by Fiber-FISH显示文摘The copy number of 5S rDNA and centromerie sequence RCS2 was determined by extended DNA fiber based fluorescence in situ hybridization (Fiber-FISH) in rice (Oryza sativa ssp. indica cv. Guangluai No. 4) genome. In order to determine the copy number, it is necessary to know the basepair number that a given length DNA fiber contains under a microscope. Therefore, the length of two DNA frag-ments, in which the basepair number had been already known, was measured. The insert sequence of the tested BAC 38D17 was 136 kb and its extended DNA was 56.4 μm long, 2.41 kb/μm on average, while that of the tested BAC 44B4 was 144.5 kb in total and 55.7 μm long, 2.60 kb/μm on average under the microscope. They were very close to the theoretical value of B-DNA in the Watson-Crick DNA model, which is 2.97 kb/μm. According to the average value of basepair number per μm of the two samples mentioned above, that is, 2.51 kb/μm, it could be estimated that the copy number was about 686 for 5S rDNA and 286-1121 for the | Zongyun Li Siluo Huang Weiwei Jin Shunbin Ning Yunchun Song Lijia Li | 2002 | Chinese Science Bulletin2002，47，3： | 5 |
| 19 | Comparison of the distribution of the repetitive DNA sequences in three variants of Cucumis sativus reveals their phylogenetic relationships显示文摘有在拷贝数字或 / 并且顺序多型性的可变性的重复 DNA 序列能作为有用分子的标记被采用学习 phylogenetics 并且当在 situ 杂交(鱼) 与荧光结合了时,识别种类 / 染色体。Cucumis sativus 有三变体, Cucumis sativus L。var。sativus, Cucumis sativus L。var。hardwickii 和 Cucumis sativus L。var。xishuangbannesis。在这三变体之中的 phylogenetics 很好没用 cytological 里程碑被探索。这里,我们在黄瓜专注于高度重复的 DNA 序列的组织和分发,与栽培变种和野黄瓜之间的差别上的强调。在黄瓜和它的亲戚的 chromosomal karyotypes 的差异在我们的学习被检测。从而,有多探查鸡尾酒的三个集合的顺序的鱼(有染色体特定的 fosmid 的联合重复 DNA 作为探针克隆) 在一样的中期房间上被进行,它帮助了我们同时识别每野黄瓜 C 的 7 个中期染色体。sativus var。hardwickii。体的中期染色体的标准化 karyotype 被构造。我们也显示的数据在栽培变种黄瓜和 C 之间的关系。s。var。xishuangbannesis 比 C 的靠近。s。var。xishuangbannesis 和 C。s。var。hardwickii。 | Xin Zhao Jingyuan Lu Zhonghua Zhang Jiajin Hu Sanwen Huang Weiwei Jin | 2011 | Journal of Genetics and Genomics2011，38，1： | 5 |
| 20 | Effect of nitrogen limitation on antioxidant qualities is highly associated with genotypes of lettuce(Lactuca sativa L.)显示文摘Antioxidants are highly beneficial to human health, and their accumulation in lettuce, one of the most popular leafy vegetables, depends on both genetic and environmental factors. Nitrogen(N) availability plays an essential role in regulating antioxidant accumulation, but the influence of genotype × N interactions on the antioxidant qualities of lettuce is poorly understood. Therefore, the present study investigated the variation of growth and antioxidant qualities of 20 lettuce(Lactuca sativa L.) genotypes(10 green lettuce genotypes and 10 red lettuce genotypes) under limited N(low N, LN) conditions and standard N(high N, HN) conditions. For all 20 genotypes, LN conditions reduced shoot(i.e., leaf) growth, but increased plant concentrations of vitamin C,glutathione, and phenolic compounds, with the exception of carotenoids, compared with HN conditions. Because of reduced biomass under LN conditions, not all lettuce genotypes exhibited increased antioxidant yields or total antioxidant capacity yield. The variation in antioxidant quality was primarily genetically determined. Generally, the green lettuce genotypes exhibited more pronounced increases in antioxidant yields and total antioxidant capacity yield than the red lettuce genotypes under LN conditions. These results suggest that even though LN conditions generally tend to improve the antioxidant qualities of lettuce, the extent of this effect is highly dependent on genotype. Therefore, genotype should be given priority in future studies that aim to improve antioxidant qualities in lettuce through N management. | Weiwei ZHOU Ting LV Yan HU Wenjing LIU Qingfang BI Chongwei JIN Lingli LU Xianyong LIN | 2020 | Pedosphere2020，30，3： | 4 |
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