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| 1 | Graft-accelerated virus-induced gene silencing facilitates functional genomics in rose flowers显示文摘Rose has emerged as a model ornamental plant for studies of flower development, senescence, and morphology, as well as the metabolism of floral fragrances and colors.Virus-induced gene silencing(VIGS) has long been used in functional genomics studies of rose by vacuum infiltration of cuttings or seedlings with an Agrobacterium suspension carrying TRV-derived vectors. However, VIGS in rose flowers remains a challenge because of its low efficiency and long time to establish silencing. Here we present a novel and rapid VIGS method that can be used to analyze gene function in rose,called ‘graft-accelerated VIGS', where axil ary sprouts are cut from the rose plant and vacuum infiltrated with Agrobacterium. The inoculated scions are then grafted back onto the plants to flower and silencing phenotypes can be observed within 5 weeks, post-infiltration. Using this new method, we successfully silenced expression of the RhDFR_1, RhA G, and RhNUDX_1 in rose flowers, and affected their color, petal number, as well as fragrance, respectively. This grafting method will facilitate high-throughput functional analysis of genes in rose flowers. Importantly, it may also be applied to other woody species that are not currently amenable to VIGS by conventional leaf or plantlet/seedling infiltration methods. | Huijun Yan Shaochuan Shi Nan Ma Xiaoqian Cao Hao Zhang Xianqin qiu Qigang Wang Hongying Jian Ningning Zhou Zhao Zhang Kaixue Tang | 2018 | Journal of Integrative Plant Biology2018，60，1： | 11 |
| 2 | Anlotinib suppresses lymphangiogenesis and lymphatic metastasis in lung adenocarcinoma through a process potentially involving VEGFR-3 signaling显示文摘Objective:Lymphatic metastasis is one of the leading causes of malignancy dispersion in various types of cancer.However,few anti-lymphangiogenic drugs have been approved for clinical use to date.Therefore,new therapies to block lymphangiogenesis are urgently required.Methods:Immunohistochemistry,immunofluorescence,Western blot,migration assays,and lymphangiogenesis and lymphatic metastasis assays were used.Results:Anlotinib,a receptor tyrosine kinase inhibitor,suppressed the rate of new metastatic lesions(31.82%in the placebo arm and 18.18%in the anlotinib arm)in patients with advanced lung adenocarcinoma who were enrolled in our ALTER-0303 study.D2-40+-lymphatic vessel density was strongly correlated with disease stage,metastasis,and poor prognosis in 144 Chinese patients with lung adenocarcinoma.In mice bearing A549EGFP tumors,tumor lymphatic vessel density,tumor cell migration to lymph nodes,and the number of distant metastatic lesions were lower in the anlotinib group than in the controls.Anlotinib inhibited the growth and migration of human lymphatic endothelial cells(hLECs)and lymphangiogenesisin vitro andin vivo.Treatment of hLECs with anlotinib downregulated phosphorylated vascular endothelial growth factor receptor 3(VEGFR-3).Conclusions:Anlotinib inhibits lymphangiogenesis and lymphatic metastasis,probably through inactivating VEGFR-3 phosphorylation.The results indicate that anlotinib may be beneficial for treatment in avoiding lymphangiogenesis and distant lymphatic metastasis in lung adenocarcinoma.(Trial registration:ALTER0303;NCT02388919;March 17,2015.) | Tingting Qin Zhujun Liu Jing Wang Junling Xia Shaochuan Liu Yanan Jia Hailin Liu Kai Li | 2020 | Cancer Biology & Medicine2020，17，3： | 9 |
| 3 | From Green Super Rice to green agriculture:Reaping the promise of functional genomics research显示文摘Producing sufficient food with finite resources to feed the growing global population while having a smaller impact on the environment has always been a great challenge.Here,we review the concept and practices of Green Super Rice(GSR)that have led to a paradigm shift in goals for crop genetic improvement and models of food production for promoting sustainable agriculture.The momentous achievements and global deliveries of GSR have been fueled by the integration of abundant genetic resources,functional gene discoveries,and innovative breeding techniques with precise gene and whole-genome selection and efficient agronomic management to promote resource-saving,environmentally friendly crop production systems.We also provide perspectives on new horizons in genomic breeding technologies geared toward delivering green and nutritious crop varieties to further enhance the development of green agricul-ture and better nourish the world population. | Sibin Yu Jauhar Ali Shaochuan Zhou Guangjun Ren Huaan Xie Jianlong Xu Xinqiao Yu Fasong Zhou Shaobing Peng Liangyong Ma Dingyang Yuan Zefu Li Dazhou Chen Ruifeng Zheng Zhigang Zhao Chengcai Chu Aiqing You Yu Wei Susong Zhu Qiongyao Gu Guangcun He Shigui Li Guifu Liu Changhua Liu Chaopu Zhang Jinghua Xiao Lijun Luo Zhikang Li Qifa Zhang | 2022 | Molecular Plant2022，15，1： | 6 |
| 4 | Significance of the lymph nodes in the 7th station in rational dissection for metastasis of distal gastric cancer with different T categories显示文摘Objective: To determine the clinicopathological characteristics, and evaluate the appropriate extent of lymph node dissection in distal gastric cancer patients with comparable T category.Methods: A retrospective study was conducted on 570 distal gastric cancer patients, who underwent gastric resection with D2 nodal dissection, which was performed by the same surgical team from January 1997 to January 2011. We compared the differences in lymph node metastasis rates and metastatic lymph node ratios between different T categories. Additionally, we investigated the impact of lymph node metastasis in the 7th station on survival rate of distal gastric cancer patients with the same TNM staging.Results: Among the 570 patients, the overall lymph node metastasis rate of advanced distal gastric cancer was 78.1%, and the metastatic lymph node ratio was 27%. The lymph node metastasis rate in the 7th station was similar to that of perigastric lymph nodes. There was no statistical significance in patients with the same TNM stage(stage II and III), irrespective of the metastatic status in the 7th station.Conclusions: Our results suggest that to a certain extent, it is reasonable to include lymph nodes in the 7th station in the D1 lymph node dissection. | Wu Song Yulong He Shaochuan Wang Weiling He Jianbo Xu | 2014 | Chinese Journal of Cancer Research2014，26，4： | 3 |
| 5 | Identification of Nonstructural Protein 8 as the N-Terminus of the RNA-Dependent RNA Polymerase of Porcine Reproductive and Respiratory Syndrome Virus显示文摘Porcine reproductive and respiratory syndrome virus (PRRSV) is a member within the family Arteriviridae of the order Nidovirales. Replication of this positive-stranded RNA virus within the host cell involves expression of viral replicase proteins encoded by two ORFs, namely ORF1 a and ORF1 b. In particular, translation of ORF1 b depends on a-1-ribosomal frameshift strategy. Thus, nonstructural protein 9 (nsp9), the first protein within ORF1 b that specifies the function of the viral RNA-dependent RNA polymerase, is expressed as the C-terminal extension of nsp8, a small nsp that is encoded by ORF1 a. However, it has remained unclear whether the mature form of nsp9 in virus-infected cells still retains nsp8,addressing which is clearly critical to understand the biological function of nsp9. By taking advantage of specific antibodies to both nsp8 and nsp9, we report the following findings. (1) In infected cells, PRRSV nsp9 was identified as a major product with a size between 72 and 95 k Da (72–95 KDa form), which exhibited the similar mobility on the gel to the in vitro expressed nsp8–9 ORF1 b, but not the ORF1 b-coded portion (nsp9 ORF1 b). (2) The antibodies to nsp8, but not to nsp7 or nsp10, could detect a major product that had the similar mobility to the 72–95 KDa form of nsp9. Moreover, nsp9 could be co-immunoprecipitated by antibodies to nsp8, and vice versa. (3) Neither nsp4 nor nsp2 PLP2 was able to cleave nsp8–nsp9 in vitro. Together, our studies provide experimental evidence to suggest that nsp8 is an N-terminal extension of nsp9.Our findings here paves way for further charactering the biological function of PRRSV nsp9. | Yuanyuan Liu Yunhao Hu Yue Chai Liping Liu Jiangwei Song Shaochuan Zhou Jia Su Lei Zhou Xinna Ge Xin Guo Jun Han Hanchun Yang | 2018 | Virologica Sinica2018，33，5： | 3 |
| 6 | Study on the vehicle routing problem of automobile logistics显示文摘 | Yun Wang Shaochuan Fu Lin Yu | | 0，，06： | 1 |
| 7 | Cryo-copolymerization preparation of dextran-hyaluronate based supermacroporous cryogel scaffolds for tissue engineering applications显示文摘Dextran-hyaluronate (Dex -- 哈) 为软织物工程的基于的 supermacroporous cryogel 脚手架被在结冰条件下面在各种各样的 macromonomer 集中包含葡聚糖 methacrylate (Dex 麻省) 和 hyaluronate methacrylate (哈麻省) 的水的答案的免费激进的 cryo-copolymerization 准备。这被观察为 Dex 的准备的 macromonomers 的合适的全部的集中 -- 哈有满足的性质的 cryogel 支架是 5%(w/w ) 在 1% 的哈麻省集中(w/v ) ,它然后被用来生产测试支架。有 5% 的获得的 cryogel 支架(w/w ) macromonomer 答案有高水位线渗透(5.1 ? 桴 ? 潨灳瑩? | Dongjiao ZHOU Shaochuan SHEN Junxian YUN Kejian YAO Dong-Qiang LIN | 2012 | Frontiers of Chemical Science and Engineering2012，6，3： | 1 |
| 8 | Field Efficacy Trial and Optimum Control Period of Rice False Smut(Ustilaginoidea virens) in Single Cropping Middle-late Rice显示文摘[Objective]The paper was to study optimum chemical agents and control periods against rice false smut( Ustilaginoidea virens) in single cropping middle-late rice. [Method]Taking chemical agents( triadimefon,armure,Jinggangmycin) as main treatments and spraying periods( within 7 d before initial heading stage,within 7 d before initial heading stage + initial heading stage,initial heading stage) as assisted treatments,the field efficacy trial and optimum control period of U. virens in single cropping middle-late rice were studied using Huanghuazhan and Jinnongsimiao as experimental materials in 2013. [Result] Three chemical agents,triadimefon,armure,Jinggangmycin,extremely reduced diseased panicle rate and disease index of U. virens,but there was no significant difference among three chemical agents. Spraying period did not have significant impact on diseased panicle rate and disease index of U. virens,whereas spraying within7 d before initial heading stage and during initial heading stage had relatively good control effect. Yield increased significantly after application of three chemical agents,and armure led to greater increase. The reason for yield increase was that seed setting rate was significantly increased,and the number of filled grains per panicle was significantly increased. Although spraying period influenced yield,the difference was not significant. [Conclusion] Triadimefon,armure and Jinggangmycin had good control effects against U. virens in single cropping middle-late rice,of which armure had better control effects. Spraying chemical agents increased seed setting rate,and further increased the number of filled grains per panicle and yield. Spraying within 7 d before initial heading stage and during initial heading stage had relatively better control effect against U. virens. | Liu Huaizhen Li Kanghuo Huang Qing Lu Xiuming Zhang Bin Li Huifeng Zou Jixiang Zhou Shaochuan | 2014 | Plant Diseases and Pests2014，5，2： | 1 |
| 9 | Study on the vehicle routing problem of automobile logistics显示文摘 | Yun Wang Shaochuan Fu Lin Yu | 2008 | Service Operations and Logistics and Informatics2008，，6： | 1 |
| 10 | The identification of Pi50(t), a new member of the rice blast resistance Pi2/Pi9 multigene family显示文摘 | Xiaoyuan Zhu Shen Chen Jianyuan Yang Shaochuan Zhou Liexian Zeng Jingluan Han Jing Su Ling Wang Qinghua Pan | 2012 | Theoretical and Applied Genetics2012，，7： | 1 |
| 11 | Coal Seam Methane Distribution and Its Significance in Pingdingshan Mining Area 显示文摘 | GUO Deyong ZHOU Xinquan SHEN Shaochuan | 2002 | Journal of Coal Science & Engineering2002，8，2： | 1 |
| 12 | Formulation of Poorly Water-Soluble Compound Loaded Solid Lipid Nanoparticles in a Microchannel System Fabricated by Mechanical Microcutting Method: Puerarin as a Model Drug显示文摘 | Linhong X Xu T Junxian Y Shaochuan S | 2012 | Industrial & Engineering Chemistry Research2012，51，11： | 1 |
| 13 | Management of cerebral palsy in children by selective posterior rhizotomy显示文摘 | PAN SHAOCHUAN | 1994 | Chinese Journal of Children Surgery1994，15，： | 1 |
| 14 | The identification of Pi50(t), a new member of the rice blast resistance Pi2/Pi9 multigene family显示文摘 | Xiaoyuan Zhu Shen Chen Jianyuan Yang Shaochuan Zhou Liexian Zeng Jingluan Han Jing Su Ling Wang Qinghua Pan | 2012 | Theoretical and Applied Genetics2012，，7： | 1 |
| 15 | Coal forming environments and their relationship to tectonic activity in the Cevennes Stephanian coal basin显示文摘 | Guo Deyong Zhou Xinquan Shen Shaochuan | 2002 | Journal of coal Science & Engineering ( China )2002，8，： | 1 |
| 16 | Chromatographic Adsorption of Serum Albumin and Antibody Proteins in Cryogels with Benzyl-Quaternary Amine Ligands显示文摘 | Junxian Yun Xiuhong Cheng Jialei Ye Shaochuan Shen Gensheng Yang Kejian Yao Harald Kirsebom Dong-Qiang Lin Yi-Xin Guan Shan-Jing Yao | 2014 | Journal of Chromatography A2014，，： | 1 |
| 17 | 美铝推出世界最轻卡车铝轮毂显示文摘近期,美国铝业公司面向市场推出新一代的铝轮毂,该种铝轮毂命名为Alcoalvlone。该铝轮毂为整体锻造产品,质量更轻,性能更好,能有效减少车轮与抱闸的磨损,是目前世界上最轻的卡车轮毂,比传统卡车轮毂轻23kg。 | Liu Honglei,Xu Bin,Ji Shaochuan,Zheng Baoquan (Citic Diacastal Wheel Group,Qinhuangdao,China) | 2010 | 特种铸造及有色合金2010，30，6： | 0 |
| 18 | Comparisons of Metabolic Profiles for Carbohydrates,Amino Acids,Lipids,Fragrance and Flavones During Grain Development in indica Rice Cultivars显示文摘We used a widely targeted metabolomics approach to examine the metabolic changes in three pedigreed indica cultivars(Meixiangzhan 2,Meisizhan and Qixinzhan),with different eating qualities,at 8,15 and 30 d after flowering(DAF)to explore the formation mechanism of rice eating and nutritional qualities at a global metabolic level.A total of 623 metabolites were identified,and results showed that metabolic variations among rice cultivars decreased with grain developmental stage,suggesting that sufficient carbohydrate and amino acid supply during grain development may contribute to excellent rice eating and nutritional quality formation.Lysophosphatidylcholines 19:0 and 16:1 were beneficial for excellent eating quality formation during grain development.Rice fragrance was attributed mainly to spermidine andγ-aminobutyric acid.Rice cultivars with excellent eating quality at 15-30 DAF had relatively higher flavone content,possibly because they had adequate carbohydrate and amino acid contents during grain development.These results provided insight into the mechanisms for establishing rice eating and nutritional qualities during grain formation at a global metabolic level,and can be applied towards improving rice quality. | CHEN Yibo WANG Zhidong WANG Chongrong LI Hong HUANG Daoqiang ZHOU Degui ZHAO Lei PAN Yangyang GONG Rong ZHOU Shaochuan | 2022 | Rice science2022，29，2： | 0 |
| 19 | Architecture modification and In^(3+)-doping of WO_(3) photoanodes to boost the photoelectrochemical water oxidation performance显示文摘WO_(3) photoanodes have been widely utilized for the oxygen evolution reaction(OER)in the photoelectrochemical water splitting system.Herein,the effects of hydrazine hydrate modification and In^(3+)-doping on the physicochemical properties and photocurrent density of the WO_(3) photoanode prepared by hydrothermally treating at 160℃followed by calcining at 500℃are investigated.Among them,the hydrazine hydrate in the hydrothermal solution can serve as a texture regulator,resulting in the formation of WO_(3) films with the layered architecture stacked by nanosheets dominantly exposed(020)facets,which allows the WO_(3) films to have faster charge separation and larger specific surface area for OER according to the characterization results of microstructures and photoelectrochemcial behaviors;while the In^(3+)-doping can optimize the energy band structure of WO_(3) and adjust the work function to increase the driving force of OER based on the ultraviolet photoelectron spectroscopy,Mott–Schottky and open-circuit photovoltage plots.Under the simulated sunlight(AM1.5G)illumination,the designed In^(3+)–WO_(3)(N_2H_4)photoanode in Na_2SO_4 solution delivers amaximum incident photon-to-current efficiency of 38.6%at 410 nm and a photocurrent density of 1.93 mA cm^(-2)at 1.23 V vs.RHE,which is 2.8 and 3.0 times higher than the pristine WO_(3) photoanode,respectively.This study provides a promising strategy to improve the water splitting performance of nanostructured WO_(3) photoanodes by altering the architecture and introducing heteroatoms. | Peng Zeng Yang Zhou Lingling Peng Shaochuan Wang Tianyou Peng | 2023 | Science China Chemistry2023，66，11： | 0 |
| 20 | Spatio-temporal epidemic type aftershock sequence model for Tangshan aftershock sequence显示文摘Shallow earthquakes usually show obvious spatio-temporal clustering patterns.In this study,several spatio-temporal point process models are applied to investigate the clustering characteristics of the well-known Tangshan sequence based on classical empirical laws and a few assumptions.The relative fit of competing models is compared by Akaike Information Criterion.The spatial clustering pattern is well characterized by the model which gives the best fit to the data.A simulated aftershock sequence is generated by thinning algorithm and compared with the real seismicity. | Shaochuan Lue Yong Li | 2011 | Earthquake Science2011，24，5： | 0 |
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