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| 1 | Establishment of a Highly Efficient Regeneration System for the Mature Embryo Culture of Wheat显示文摘Establishment of a highly efficient regeneration system for the mature embryo of wheat will provide a convenient tool for wheat tissue culture and transformation, thereby facilitating the transformation of foreign genes into wheat. By using the mature embryos derived from 20 different wheat lines including Shi 4185, Yumai 66, Lunxuan 987, CB037, Yangmai 6, Xinchun 9, Bobwhite, Han 6172, Zheng 9023, Jimai 20, Ningchun 4, and Jing 411, the effects of some factors including inoculation methods, initiating culture media, organic additives, antioxidants, and auxins on the regeneration from the explants were evaluated. The results indicated that the scraping embryo culture was better than the whole embryo culture, the Aa medium was better than the SD2 medium and dicamba was better than 2,4-D in increasing the regeneration frequency. An Adi medium was established in this study by adding silver nitrate, cysteine, ascorbic acid, dicamba, glutamine into the Aa medium at the concentration of 4 , 40, 100, 2, and 5 mg L-1, respectively. By using the Adi medium and the scraping technique, the regeneration frequencies of the mature embryos of CB037, Lunxuan 987, Han 6172, Yangmai 6, Bobwhite, Zheng 9023, Shi 4185, and Jimai 20 became 85.6, 60.1, 46.0, 42.1, 42.0, 34.0, 33.0, and 32.0%, respectively, which were about 5-8 times higher than that obtained from the conventional culture mediums and techniques. This novel regeneration system could be helpful in wheat transformation. | YIN Gui-xiang WANG Yan-li SHE Mao-yun DU Li-pu XU Hui-jun MA Jing-xiu YE Xing-guo | 2011 | Agricultural Sciences in China2011，10，1： | 19 |
| 2 | Improvement of Plant Regeneration from Immature Embryos of Wheat Infected by Agrobacterium tumefaciens显示文摘Wheat, one of the most important food crops, has been extensively studied with respects to plant regeneration and transformation employing the immature embryos as recipient tissues. However, the transformed tissues often become severely necrotic after co-cultivation with Agrobacterium, which is one of the major obstacles in gene delivery. In this study, wheat varieties CB037, Kenong 199, Xinchun 9, Lunxuan 987, and Shi 4185 showed desirable culture potential or high infection ability in Agrobacterium-mediated transformation. Similarly, optimal regeneration conditions were determined by testing their ability to inhibit the cell necrosis and cell death phenotype. Two auxins of 2,4-dichlorophenoxyacetic acid (2,4-D) and 3,6-dichloro-o-anisic acid (dicamba) were evaluated for highly significant effect on both callus and plantlet production, although they were genotype-dependent in wheat. Substitution of 2,4-D by dicamba enhanced the growth and regeneration ability of callus from the immature embryos of most genotypes tested. The callus growth state couldn’t be modified by adding antioxidants such as ascorbic acid, cysteine, and silver nitrate or organic additives such as thiamine HCl and asparagine to the media. On the contrary, the best tissue statement and plant regeneration was achieved by employing the media containing the simplest MS (Murashige and Skoog) components and dicamba without organic components and vitamins. Thereby, our results are thought to inhibit wheat cell necrosis effectively and suggested to be used for more wheat genotypes. | TAO Li-li YIN Gui-xiang DU Li-pu SHI Zheng-yuan SHE Mao-yun XU Hui-jun YE Xing-guo | 2011 | Agricultural Sciences in China2011，10，3： | 10 |
| 3 | Cloning and Characterization of Genes Coding for Fructan Biosynthesis Enzymes (FBEs) in Triticeae Plants显示文摘Fructan is not only a carbon source for storage but also plays an important role as anti-stress agents in many plant species. Complex fructans having both β-(2,1)- and β-(2,6)-linked fructosyl units accumulate in Triticeae plants commonly. Three enzymes (sucrose: sucrose 1-fructosyltransferase, 1-SST, EC: 2.4.1.99; sucrose: fructan 6-fructosyltransferase, 6- SFT, EC: 2.4.1.10; and fructan: fructan 1-fructosyltransferase, 1-FFT, EC: 2.4.1.100) were involved in fructan biosynthesis in Triticeae plant species. We successfully isolated these genes from tetraploid wheat (Triticum turgidum, genotype: AABB), common wheat (Triticum aestivum L., genotype: AABBDD) and three wild relatives of common wheat, Triticum urartu Thum. (the origin of the AA genome), Aegilops speltoides (Tausch) Gren. (the putative source of the SS genome) and Aegilops tauschii Coss. (the source of the DD genome). Sequence analysis revealed that all the FBEs (fructan biosynthetic enzymes) had three highly conserved functional motifs except 1-SST (EU981912) from tetraploid wheat species only with conserved DPNG. Low pI (isoelectric point) and potential N-glycosylation sites were predicted, which were crucial for protein compartmentation and post-translational process. Analysis on subcelluar localization signals showed that only 6-SFT had vacuolar-directed signal. Sequences alignment result showed that 1-SST and 1-FFT were more conservative and had closer relationship each other, while 6-SFT was more active during the evolution processing. According to the syntenic relationship between wheat and rice genome, FBEs were predicated to be located on the homeologous group 6 and group 2 chromosomes. Expression profile confirmed that expression of all the three FBEs were drought-stress induced. This study can assist to establish a useful theoretical platform for cold- or drought-tolerant improvement of wheat by modulating FBEs expression. | GAO Xiang SHE Mao-yun YIN Gui-xiang YU Yang QIAO Wei-hua DU Li-pu YE Xing-guo | 2010 | Agricultural Sciences in China2010，9，3： | 9 |
| 4 | Pedicled greater omentum flap for preventing bile leak in liver transplantation patients with poor biliary tract conditions显示文摘BACKGROUND:Bile leak remains a main complication in liver transplantation patients with poor biliary tract conditions,mainly caused by an insufficient blood supply or dysplasia of the biliary tract.Although Roux-en-Y modus operandi can be adopted,the risk of other complications of the biliary tract such as infection increases.Using pedicled greater omentum flaps to wrap the anastomotic stoma, which increases the biliary tract blood supply,may reduce the incidence of bile leak. METHODS:Fourteen patients undergoing piggy-back liver transplantation and having poor biliary tract conditions were treated with pedicled greater omentum flaps to wrap the anastomotic stoma of the biliary tract. Their clinical data were analyzed retrospectively. RESULTS:Of the 14 patients,only one(7.1%)had a mild bile leak on the 8th day post-operation and fully recovered after symptomatic treatment.The other patients had no biliary complications. CONCLUSIONS:Using pedicled greater omentum flaps to wrap the anastomotic stoma of the biliary tract is an effective way to prevent bile leak in liver transplantation patients,especially those with poor biliary tract conditions.However,experience with this surgical technique still needs to be further explored. | Ye, Qi-Fa Niu, Ying She, Xing-Guo Ming, Ying-Zi Cheng, Ke Ma, Yin Ren, Zu-Hai | 2007 | Hepatobiliary & Pancreatic Diseases International2007，6，5： | 5 |
| 5 | Hepatic veins anatomy and piggy-back liver transplantation显示文摘BACKGROUND:The piggy-back caval anastomosis technique is widely used in orthotopic liver transplantation although it carries an increased risk of complications,including outflow obstruction and Budd-Chiari syndrome.The aim of this study is to clarify the anatomy and variations of hepatic veins(HVs) draining into the inferior vena cava(IVC),and to classify the surgical techniques of piggy-back liver transplantation(PBLT) based on the anatomy of HVs which can reduce the occurrence of complications.METHODS:PBLT was performed in 248 consecutive cases at our hospital from January 2004 to August 2011.The anatomy of recipients' HVs was determined when removing the native diseased livers.Both anatomy of HVs and short HVs draining into the IVC were recorded.These data were collected and analyzed.RESULTS:We classified anatomic variations of HVs in the 248 livers into five types according to the way of drainage into the IVC:type I(trunk type of left and middle HVs),142(57.3%) patients;type II(trunk type of right and middle HVs),54(21.8%);type III(trunk type of left,middle and right HVs),14(5.6%);type IV(non-trunk type of left,middle and right HVs),of which,type IVa,16(6.5%),in the same horizontal plane;type IVb,18(7.3%),in different horizontal planes;and type V(segment type),4(1.6%).The patients whose HVs anatomy belonged to types I,II and III underwent classical piggy-back liver transplantation.Type IVa patients had classical PBLT via HV venoplasty prior to piggy-back anastomosis,while type IVb patients and type V patients could only have modified PBLT.CONCLUSION:This study demonstrates that HVs can be classified according to the anatomy of their drainage into the IVC and we can use this classification to choose the best operative approach to PBLT. | Ying-Zi Ming,Ying Niu,Ming-Jie Shao,Xing-Guo She and Qi-Fa Ye Research Center of Chinese Health Ministry on Transplantation Medicine Engineering and Technology,The Third Xiangya Hospital,Central South University,Changsha 410013,China | 2012 | Hepatobiliary & Pancreatic Diseases International2012，11，4： | 4 |
| 6 | Risk factors for delayed graft function in cardiac death donor renal transplants显示文摘 | SHAO Ming-jie YE Qi-fa MING Ying-zi SHE Xing-guo LIU Hong YE Shao-jun NIU Ying | 2012 | Chinese Medical Journal2012，，21： | 4 |
| 7 | Outcome of kidney transplantation between controlled cardiac death and brain death donors: a meta-analysis显示文摘 | Ming Yingzi Shao Mingjie Tian Tingting She Xingguo Liu Hong Ye Shaojun Ye Qifa | 2014 | Chinese Medical Journal2014，，15： | 3 |
| 8 | Simultaneous detection and characterization of toxigenic Clostridium difficile directly from clinical stool specimens显示文摘我们采用了结合的复合聚合酶链反应(PCR ) 指向六的毛状的电气泳动(mPCR-CE ) 在为 toxigenic C 的同时的察觉和描述的 tcdC 的 Clostridium 顽固基因,包括的 tpi, tcdA, tcdB, cdtA, cdtB,和删除。顽固直接从烘便的标本。mPCR-CE 每反应有 10 个形成殖民地的单位的察觉的限制没有与另外的相关细菌的基因跨反应。临床的确认在 354 上被执行连续地与怀疑的 C 从病人收集了凳子标本。顽固感染并且 45 孤立。结果与与 BD 最大 Cdiff,即时房间分析试金(RTCA ) ,和 mPCR-CE 相结合的一个参考标准相比。toxigenic C。顽固种类在 36 被检测孤立并且由 mPCR-CE 的 45 个凳子标本,它提供了 20.3% 的积极的率(81/399 ) 。mPCR-CE 有 97.2% 的特性和 96.0% 的敏感,它比 RTCA 高(x 2= 5.67, P = 0.017 ) 但是比 BD 最大 Cdiff 低(P = 0.245 ) 。在 45 紧张之中,(97.8%) 44 作为 non-ribotype 被决定 027 由 mPCR-CE,它充分被适合于 PCR ribotyping。尽管 ribotypes 017 (n = 8, 17.8%) , 001 (n = 6, 13.3%) ,并且 012 (n = 7, 15.6%) 在这个区域是占优势的, ribotype 027 重要遗传型习惯性地被监视。mPCR-CE 为 toxigenic C 的同时的察觉提供了一个其他的诊断工具。在凳子顽固、在 RT027 和 non-RT027 之间潜在地区分。 | Hanjiang Lai Chen Huang Jian Cai Julian Ye Jun She Yi Zheng Liqian Wang Yelin Wei Weijia Fang Xianjun Wang Yi-Wei Tang Yun Luo Dazhi Jin | 2018 | Frontiers of Medicine2018，12，2： | 2 |
| 9 | T he BAD protein integrates survival signaling by egfr/mapk and PI 3 K/akt kinase pathways in pten-deficient tumor ceils 显示文摘 | She QB Solit DB Ye Q | 2005 | Cancer Cell2005，8，4： | 1 |
| 10 | Rapid response of brain metastasis to crizotinib in a patient with KLC1-ALK fusion and MET gene amplification positive non-small cell lung cancer:a case report显示文摘Non-small cell lung cancer(NSCLC) ranks as the leading cause of cancer-related death in the world. Brain metastasis(BM) is a common complication of NSCLC, with 25%–40% of patients developing BM during the course of the disease. A significant strategy of local disease control in the central nervous system is radiation therapy. With the development of precision medicine,the concept of treating lung cancer BM has gradually changed. In this case, we performed a surgical procedure to obtain enough tumor tissue for the detection of the target gene and other related experiments after the patient was informed. Finally, we found that the patient had both hepatocyte growth factor receptor(MET) gene amplification and kinesin light chain 1-anaplastic lymphoma kinase fusion(KLC1-ALK) through next-generation sequencing and showed sensitivity to the targeted therapy of crizotinib. The patient exhibited good response. Our case was successful and underwent targeted therapy with the guidance of precise diagnosis. | Peng Wang Pei Xiao Yingnan Ye Pengpeng Liu Lei Han Li Dong Chunhua She Jinpu Yu | 2017 | Cancer Biology & Medicine2017，14，2： | 1 |
| 11 | The BAD protein integrates survival signling by EGFR/MAPK and PI3K/Akt kinase pathways in PTEN-deficient tumor cells显示文摘 | Qing-Bai She David B Solit Qing Ye | 2005 | Cancer Cell2005，8，： | 1 |
| 12 | 4E-BP1 is a key effector of the oncogenic activation of the AKT and ERK signaling pathways that integrates their function in tumors显示文摘 | She QB Halilovic E Ye Q | 2010 | Cancer Cell2010，18，1： | 1 |
| 13 | mansoni 显示文摘 | Ruppel A She YE Wei DX Sere of Schistosoma Japon/cum Infected Patients Cross - react with Diagnostic 31/ 31 kDa Proteins of S | 1987 | Clin Exp Immunol1987，69，： | 1 |
| 14 | Breast tumor cens with PI3K mutation or Her-2 amplification are selectively addicted to Akt signaling显示文摘 | She QB Chandarlapaty S Ye Q | 2008 | PLoS One2008，3，8： | 1 |
| 15 | Breast tumor cells with PI3K mutation or HER2 amplification are selectively addicted to AKT signaling 显示文摘 | She QB Chandarlapaty S Ye Q Lobo J Haskell KM Leander KR | 2008 | PLoS One2008，3，8： | 1 |
| 16 | Advances in post-operative prognostic models for hepatocellular carcinoma显示文摘Hepatocellular carcinoma(HCC)is one of the most common malignancies and a leading cause of cancer-related death worldwide.Surgery remains the primary and most successful therapy option for the treatment of early-and mid-stage HCCs,but the high heterogeneity of HCC renders prognostic prediction challenging.The construction of relevant prognostic models helps to stratify the prognosis of surgically treated patients and guide personalized clinical decision-making,thereby improving patient survival rates.Currently,the prognostic assessment of HCC is based on several commonly used staging systems,such as Tumor-Node-Metastasis(TNM),Cancer of the Liver Italian Program(CLIP),and Barcelona Clinic Liver Cancer(BCLC).Given the insufficiency of these staging systems and the aim to improve the accuracy of prognostic prediction,researchers have incorporated further prognostic factors,such as microvascular infiltration,and proposed some new prognostic models for HCC.To provide insights into the prospects of clinical oncology research,this review describes the commonly used HCC staging systems and new models proposed in recent years. | Ziqin HE Xiaomin SHE Ziyu LIU Xing GAO Lu LU Julu HUANG Cheng LU Yan LIN Rong LIANG Jiazhou YE | 2023 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2023，24，3： | 1 |
| 17 | The BAD protein integrates survival signaling by EGFR/MAPKand PI3K/Akt kinase pathways in PTEN-deficient tumor cells 显示文摘 | Qing-Bai She David B Solit Qing Ye | 2005 | Cancer Cell2005，8，10： | 1 |
| 18 | Up-regulation of IL-6 and TNF-α induced by SARS-coronavirus spike protein in murine macrophages via NF-κB pathway显示文摘 | Wei Wang Linbai Ye Li Ye Baozong Li Bo Gao Yingchun Zeng Lingbao Kong Xiaonan Fang Hong Zheng Zhenghui Wu Yinglong She | 2007 | Virus Research2007，，1： | 1 |
| 19 | The BAD protein integrates survival signaling by EGFR/MAPK and PI3K/Akt kinase pathways in PTEN- deficient tumor cells显示文摘 | She Q B Solit D B Ye Q | 2005 | Cancer Cell2005，8，4： | 1 |
| 20 | A unified formulation for hollow and solid concrete-filled steel tube columns under axial compression 显示文摘 | YU Min ZHA Xiao-xiong YE Jian-qiao SHE Chun-yan | 2010 | Journal of Engineering Structures2010，32，4： | 1 |
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