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| 1 | Humoral immune response to circulating SARS-CoV-2 variants elicited by inactivated and RBD-subunit vaccines显示文摘SARS-CoV-2 variants could induce immune escape by mutations on the receptor-binding domain(RBD)and N-terminal domain(NTD).Here we report the humoral immune response to circulating SARS-CoV-2 variants,such as 501Y.V2(B.1.351),of the plasma and neutralizing antibodies(NAbs)elicited by CoronaVac(inactivated vaccine),ZF2001(RBD-subunit vaccine)and natural infection.Among 86 potent NAbs identified by high-throughput single-cell VDJ sequencing of peripheral blood mononuclear cells from vaccinees and convalescents,near half anti-RBD NAbs showed major neutralization reductions against the K417N/E484K/N501Y mutation combination,with E484K being the dominant cause.VH3-53/VH3-66 recurrent antibodies respond differently to RBD variants,and K417N compromises the majority of neutralizing activity through reduced polar contacts with complementarity determining regions.In contrast,the 242–244 deletion(242–244Δ)would abolish most neutralization activity of anti-NTD NAbs by interrupting the conformation of NTD antigenic supersite,indicating a much less diversity of anti-NTD NAbs than anti-RBD NAbs.Plasma of convalescents and CoronaVac vaccinees displayed comparable neutralization reductions against pseudo-and authentic 501Y.V2 variants,mainly caused by E484K/N501Y and 242–244Δ,with the effects being additive.Importantly,RBD-subunit vaccinees exhibit markedly higher tolerance to 501Y.V2 than convalescents,since the elicited anti-RBD NAbs display a high diversity and are unaffected by NTD mutations.Moreover,an extended gap between the third and second doses of ZF2001 leads to better neutralizing activity and tolerance to 501Y.V2 than the standard three-dose administration.Together,these results suggest that the deployment of RBD-vaccines,through a third-dose boost,may be ideal for combating SARS-CoV-2 variants when necessary,especially for those carrying mutations that disrupt the NTD supersite. | Yunlong Cao Ayijiang Yisimayi Yali Bai Weijin Huang Xiaofeng Li Zhiying Zhang Tianjiao Yuan Ran An Jing Wang Tianhe Xiao Shuo Du Wenping Ma Liyang Song Yongzheng Li Xiang Li Weiliang Song Jiajing Wu Shuo Liu Xuemei Li Yonghong Zhang Bin Su Xianghua Guo Yangyang Wei Chuanping Gao Nana Zhang Yifei Zhang Yang Dou Xiaoyu Xu Rui Shi Bai Lu Ronghua Jin Yingmin Ma Chengfeng Qin Youchun Wang Yingmei Feng Junyu Xiao Xiaoliang Sunney Xie | 2021 | Cell Research2021，31，7： | 12 |
| 2 | Graphitic carbon nitride (g-C3N4)-based nanosized heteroarrays: Promising materials for photoelectrochemical water splitting显示文摘Photoelectrochemical(PEC)water splitting is recognized as a sustainable strategy for hydrogen generation due to its abundant hydrogen source,utilization of inexhaustible solar energy,high-purity product,and environment-friendly process.To actualize a practical PEC water splitting,it is paramount to develop efficient,stable,safe,and low-cost photoelectrode materials.Recently,graphitic carbon nitride(g-C3N4)has aroused a great interest in the new generation photoelectrode materials because of its unique features,such as suitable band structure for water splitting,a certain range of visible light absorption,nontoxicity,and good stability.Some inherent defects of g-C3N4,however,seriously impair further improvement on PEC performance,including low electronic conductivity,high recombination rate of photogenerated charges,and limited visible light absorption at long wavelength range.Construction of g-C3N4-based nanosized heteroarrays as photoelectrodes has been regarded as a promising strategy to circumvent these inherent limitations and achieve the high-performance PEC water splitting due to the accelerated exciton separation and the reduced combination of photogenerated electrons/holes.Herein,we summarize in detail the latest progress of g-C3N4-based nanosized heteroarrays in PEC water-splitting photoelectrodes.Firstly,the unique advantages of this type of photoelectrodes,including the highly ordered nanoarray architectures and the heterojunctions,are highlighted.Then,different g-C3N4-based nanosized heteroarrays are comprehensively discussed,in terms of their fabrication methods,PEC capacities,and mechanisms,etc.To conclude,the key challenges and possible solutions for future development on g-C3N4-based nanosized heteroarray photoelectrodes are discussed. | Liqun Wang Wenping Si Yueyu Tong Feng Hou Daniele Pergolesi Jungang Hou Thomas Lippert Shi Xue Dou Ji Liang | 2020 | Carbon Energy2020，2，2： | 10 |
| 3 | Characterization of the essential role of bone morphogenetic protein 9 (BMP9) in osteogenic differentiation of mesenchymal stem cells (MSCs) through RNA interference显示文摘Mesenchymal stem cells(MSCs)are multipotent stem cells and capable of differentiating into multiple cell types including osteoblastic,chondrogenic and adipogenic lineages.We previously identified BMP9 as one of the most potent BMPs that induce osteoblastic differentiation of MSCs although exact molecular mechanism through which BMP9 regulates osteogenic differentiation remains to be fully understood.Here,we seek to develop a recombinant adenovirus system to optimally silence mouse BMP9 and then characterize the important role of BMP9 in osteogenic differentiation of MSCs.Using two different siRNA bioinformatic prediction programs,we design five siRNAs targeting mouse BMP9(or simB9),which are expressed under the control of the converging H1 and U6 promoters in recombinant adenovirus vectors.We demonstrate that two of the five siRNAs,simB9-4 and simB9-7,exhibit the highest efficiency on silencing exogenous mouse BMP9 in MSCs.Furthermore,simB9-4 and simB9-7 act synergistically in inhibiting BMP9-induced expression of osteogenic markers,matrix mineralization and ectopic bone formation from MSCs.Thus,our findings demonstrate the important role of BMP9 in osteogenic differentiation of MSCs.The characterized simB9 siRNAs may be used as an important tool to investigate the molecular mechanism behind BMP9 osteogenic signaling.Our results also indicate that recombinant adenovirus-mediated expression of siRNAs is efficient and sustained,and thus may be used as an effective delivery vehicle of siRNA therapeutics. | Shujuan Yan Ruyi Zhang Ke Wu Jing Cui Shifeng Huang Xiaojuan Ji Liping An Chengfu Yuan Cheng Gong Linghuan Zhang Wei Liu Yixiao Feng Bo Zhang Zhengyu Dai Yi Shen Xi Wang Wenping Luo Bo Liu Rex C.Haydon Michael J.Lee Russell R.Reid Jennifer Moriatis Wolf Qiong Shi Hue H.Luu Tong-Chuan He Yaguang Weng | 2018 | Genes & Diseases2018，5，2： | 8 |
| 4 | Effect of NH_4F and Nano-SiO_2 on Morphological Control of α-Al_2O_3 Platelets via Solid-state Reaction显示文摘Alpha-alumina(α-Al_2O_3) platelets were prepared via solid-state reaction using pseudo-boehmite as the starting material, while NH_4F and nano-SiO_2 were used as additives, and nitric acid was used as the binder. The effect of these additives on properties of the alumina platelets was determined via scanning electron microscopy, X-ray diffraction, and measurements of the surface area and silicon content. A mechanism governing their role in the synthesis process was proposed. The results indicated that NH_4F could promote the formation of highly crystalline platelets. Addition of nano-SiO_2 could lead to increase in the diameter and reduction in the thickness of the platelets, and could also prevent their transformation to α-Al_2O_3. This addition weakened the effect of NH_4F because of the reaction of nano-SiO_2 with F-species and the formation of volatile SiF_4. | Miao Zhuang Shi Jiangong Zhang Wenping Zhang Minhong | 2016 | China Petroleum Processing & Petrochemical Technology2016，18，4： | 7 |
| 5 | Optimal stimulation parameters for Renzhong (DU 26) electro-acupuncture for improving motor function in a rat model of middle cerebral artery occlusion显示文摘The selection of electro-acupuncture parameters remains poorly unified between clinical studies.The present study observed the effects of electro-acupuncturing Renzhong (DU 26) with different stimulation parameters on motor function recovery following middle artery occlusion injury in rats.Results showed an optimal stimulation parameter for Renzhong electro-acupuncture that was low frequency and mild current (2 Hz,1 mA) significantly improved cortical excitability and conductive function,and promoted recovery in a rat model of motor function in middle artery occlusion.Frequency had a greater impact than current or interaction,and played a critical role in electro-acupuncture therapy. | Lin Han Shu Wang Jinquan Ma Wenping Yao Yan Shen Xuemin Shi | 2011 | Neural Regeneration Research2011，6，12： | 5 |
| 6 | Pore evolution characteristic of shale in the Longmaxi Formation,Sichuan Basin显示文摘Through the field emission scanning electronic microscope(FESEM)and the nitrogen adsorption test,pore type and structure of shale reservoir in the Longmaxi Formation in the Sichuan Basin were well studied.Result showed that the pore type includes organic pore,intercrystalline pore,dissolution intracrystalline pore and interparticle pore,and the organic pore was one of major pore types;among the organic pore,the micropore had large pore volume and specific surface area,and was the main storage space of shale gas.Through study on effect of total organic carbon(TOC),organic matter maturity(Ro),diagenesis and tectonism on shale porosity,influence of TOC on porosity could be divided into four stages:the rapid increasing stage(TOC from 0 to 2%),the slow decreasing stage(TOC from 2 to 3%),the rapid increasing stage(TOC from 3 to 4%or 6%)and the rapid decreasing stage(TOC>4%or 6%);influence of the maturity on porosity of shale could be divided into three stages:the rapid decreasing stage(Ro from 1.5 to 2.2%),the rapid increasing stage(Ro from 2.2 to 2.7%)and the rapid decreasing stage(Ro>2.7%);during the high thermal evolution stage,the organic diagenesis was stronger than the inorganic diagenesis;the tectonism had a great impact on porosity,and the more intense the tectonism was,the smaller the porosity would be.The evolution of shale porosity of the Longmaxi Formation underwent five stages:the immature rapid compaction stage(Ro<0.7%),the mature hydrocarbon generation and dissolution stage(Ro from 0.7 to 1.3%),the high mature pore closed stage(Ro from 1.3 to 2.2%),the overmature secondary pyrolysis stage(Ro from 2.2 to 2.7%)and the overmature slow compaction stage(Ro>2.7%);among which the mature hydrocarbon generation and dissolution stage and the overmature secondary pyrolysis stage were the most favorable shale pore development stages。 | Wenping Liu Jun Liu Molun Cai Chao Luo Xuewen Shi Jian Zhang | 2017 | Petroleum Research2017，2，4： | 3 |
| 7 | Application of contrast-enhanced ultrasound in minimally invasive ablation of benign thyroid nodules显示文摘Objective:This study aimed to investigate the application value of contrast-enhanced ultrasound(CEUS)before and after minimally invasive ablation procedures for benign thyroid nodule(s)(BTN).Methods:This prospective study included patients with BTNs scheduled to undergo ultrasound-guided minimally invasive ablation treatment.CEUS was performed before and after ablation(at 1 day,and 1,6,and 12 months after ablation).Changes in microvascular perfusion and the volume of BTNs were noted and assessed.Results:Sixty-two patients(62 BTNs),who underwent ablation procedures between June 2016 and August 2020,were included.All lesions were confirmed by biopsy,and histopathological results were obtained before ablation treatment.On preoperative CEUS,the lesions exhibited hyperenhancement(53.23%)or iso-enhancement(46.77%)during the arterial phase,and all lesions exhibited iso-enhancement in the venous and late phases.One day after ablation,none of the BTNs exhibited obvious enhancement on CEUS.One(1.61%)lesion was retreated due to a nodule-like enhancement area detected by CEUS at the 6-month follow-up.The mean nodular volume reduction rate(VRR)at 1,6,and 12 months follow-up demonstrated no significant difference between the two ablation groups(microwave ablation versus radiofrequency ablation).Twelve months after ablation,the mean(±SD)VRR of all BTNs was 60.3±10.3%.Conclusion:CEUS helped guide treatment decisions for BTNs before ablation treatment.Moreover,it could also be used to accurately and noninvasively evaluate treatment efficacy. | Jiaying Cao Peili Fan Feihang Wang Shuainan Shi Lingxiao Liu Zhiping Yan Yi Dong Wenping Wang | 2022 | Journal of Interventional Medicine2022，5，1： | 3 |
| 8 | Present situation of production process and market of methyl methacrylate at home and abroad 显示文摘 | CUI BO SHI WENPING | 2000 | Technique and Economics of Petrochemical Engynology2000，8，6： | 1 |
| 9 | Deactivation and regeneration of TS-1/SiO2 catalyst for epoxidation of propylene with hydrogen peroxide in a fixed-bed reactor显示文摘为有在一个改正床反应堆的氢过氧化物的丙烯的 epoxidation 的 TS-1/SiO2 催化剂被调查了。逐渐地,与丙烯的联机反应时间,而是选择, epoxide 在大约 93% 点被保留的催化剂活动减少。新鲜、撤销并且再生的催化剂与 X 光检查衍射被描绘, Fourier 变换红外线的光谱学, ultra-violet-visible 弥漫的反射, Brunner-Emmett-Teller 方法和 thermogravimetric 分析,和撤销催化剂与 H2O2/methanol 被改革溶液。与新鲜催化剂相比,框架结构和在撤销并且再生的 TS-1/SiO2 催化剂的框架的钛的内容没被改变。催化剂释放的主要原因是由笨重器官的副产品的催化剂的隧道的阻塞,它在 TS-1 盖住钛的活跃中心。撤销 TS-1/SiO2 催化剂上的扔的材料能被处理与氢过氧化物 / 甲醇答案或纯甲醇移开;更高处理温度并且越高在甲醇的 H2O2 的集中,越 higher 新生的程度。新生处理没在丙烯 epoxidation 影响产品选择。 | Hainan SHI Yaquan WANG Guoqiang WU Wenping FENG Yi Lin Teng ZHANG Xing JIN Shuhai WANG Xiaoxue WU Pengxu YAO | 2013 | Frontiers of Chemical Science and Engineering2013，7，2： | 1 |
| 10 | A Brillouin lidar system using F-P etalon and ICCD for remote sensing of the ocean 显示文摘 | SHI Jinwei OUYANG Min GONG Wenping | 2008 | Applied Physics B(S0946-2171)2008，90，34： | 1 |
| 11 | Present situation of production pr- ocess and market of methyl methaerylate at home and a- broad 显示文摘 | Cui bo Shi wenping | 2000 | Technique and Economics of Petrochemical Engynology2000，8，6： | 1 |
| 12 | Effect of Lithium Nitrate on the Structure and Property of α-Al2O3 Platelets Prepared via Solid-state Reaction显示文摘The α-Al_2O_3 platelets were prepared via solid-state reactions and the effect of the amount of lithium nitrate additive on the property of the platelets was investigated. The ICP results indicated that the high temperature calcination process resulted in a large loss of lithium species because of volatilization, but there was still a small amount of residual lithium species in the α-Al_2O_3 platelets. The SEM micrographs showed that lithium nitrate led to decrease in the thickness of α-Al_2O_3 platelets and irregular morphology of aggregates. Pore structures results exhibited that addition of lithium nitrate led to decrease in the pore size and increase in the specific surface area of aggregates of α-Al_2O_3 platelets. The XRD and IR patterns suggested that the residual lithium and aluminum oxide formed LiAl_5O_8. The existence of LiAl_5O_8 was the basic reason for the changed performance of α-Al_2O_3 platelets. | Miao Zhuang Shi Jiangong Miao Qingyuan Hao Jianwei Zhang Yi Zhang Wenping | 2017 | China Petroleum Processing & Petrochemical Technology2017，19，2： | 1 |
| 13 | High performance adsorbents based on hierarchically porous silica for purifying muhieomponent wastewater 显示文摘 | Shi Wenping Sheng Yang | 2011 | Journal of Materials Chemistry2011，21，15： | 1 |
| 14 | A non-ACE2 competing human single-domain antibody confers broad neutralization against SARS-CoV-2 and circulating variants显示文摘The current COVID-19 pandemic has heavily burdened the global public health system and may keep simmering for years.The frequent emergence of immune escape variants have spurred the search for prophylactic vaccines and therapeutic antibodies that confer broad protection against SARS-CoV-2 variants.Here we show that the bivalency of an affinity maturated fully human singledomain antibody(n3113.1-Fc)exhibits exquisite neutralizing potency against SARS-CoV-2 pseudovirus,and confers effective prophylactic and therapeutic protection against authentic SARS-CoV-2 in the host cell receptor angiotensin-converting enzyme 2(ACE2)humanized mice.The crystal structure of n3113 in complex with the receptor-binding domain(RBD)of SARS-CoV-2,combined with the cryo-EM structures of n3113 and spike ecto-domain,reveals that n3113 binds to the side surface of up-state RBD with no competition with ACE2.The binding of n3113 to this novel epitope stabilizes spike in up-state conformations but inhibits SARS-CoV-2 S mediated membrane fusion,expanding our recognition of neutralization by antibodies against SARS-CoV-2.Binding assay and pseudovirus neutralization assay show no evasion of recently prevalent SARS-CoV-2 lineages,including Alpha(B.1.1.7),Beta(B.1.351),Gamma(P.1),and Delta(B.1.617.2)for n3113.1-Fc with Y58L mutation,demonstrating the potential of n3113.1-Fc(Y58L)as a promising candidate for clinical development to treat COVID-19. | Zhenlin Yang Yulu Wang Yujia Jin Yuanfei Zhu Yanling Wu Cheng Li Yu Kong Wenping Song Xiaolong Tian Wuqiang Zhan Ailing Huang Shanshan Zhou Shuai Xia Xiaoxu Tian Chao Peng Cuicui Chen Yibing Shi Gaowei Hu Shujuan Du Yuyan Wang Youhua Xie Shibo Jiang Lu Lu Lei Sun Yuanlin Song Tianlei Ying | 2021 | Signal Transduction and Targeted Therapy2021，6，12： | 1 |
| 15 | A lider system based on stimulated brillouin scattering 显示文摘 | Shi Jinwei Li Guixin Gong Wenping | 2007 | Appl Phys B2007，86，： | 1 |
| 16 | Highly active pd/WO2-CNTs catalysts for formic acid electro-oxidation and study of the kinetics显示文摘 | Chun'an Ma* Yanxian Jin Meiqin Shi Youqun Chu Yinghua Xu Wenping Jia Qiaohua Yuan Jiabin Chen Huiling Pan Qiuwei Dai | 2014 | Ionics2014，20，： | 1 |
| 17 | Real-time detecting of Brillouin scattering in water with ICCD显示文摘 | Li Shujing Shi jinwei Gong Wenping | 2005 | Proc SPIE2005，6028，： | 1 |
| 18 | Downregulation of L?type amino acid transporter 1 expression inhibitsthe growth, migration and invasion of gastric cancer cells显示文摘 | Lianghui Shi Wenping Luo Wenbing Huang Shisui Huang Guangyan Huang | 2013 | Oncology Letters2013，，1： | 1 |
| 19 | Real - time Detecting of Brillouin Scattering in Water with ICCD 显示文摘 | Li Shujing Shi jinwei Gong Wenping | 2005 | Proceedings of 20th Congress of the International Commission for Optics [C]2005，，： | 1 |
| 20 | Design and experiment of a novel pneumatic soft arm based on a deployable origami exoskeleton显示文摘T Soft arms have shown great application potential because of their flexibility and compliance in unstructured environments.However,soft arms made from soft materials exhibit limited cargo-loading capacity,which restricts their ability to manipulate objects.In this research,a novel soft arm was developed by coupling a rigid origami exoskeleton with soft airbags.The joint module of the soft arm was composed of a deployable origami exoskeleton and three soft airbags.The motion and load performance of the soft arm of the eight-joint module was tested.The developed soft arm withstood at least 5 kg of load during extension,contraction,and bending motions;exhibited bistable characteristics in both fully contracted and fully extended states;and achieved a bending angle of more than 240°and a contraction ratio of more than 300%.In addition,the high extension,contraction,bending,and torsional stiffnesses of the soft arm were experimentally demonstrated.A kinematic-based trajectory planning of the soft arm was performed to evaluate its error in repetitive motion.This work will provide new design ideas and methods for flexible manipulation applications of soft arms. | Yuwang LIU Wenping SHI Peng CHEN Yi YU Dongyang ZHANG Dongqi WANG | 2023 | Frontiers of Mechanical Engineering2023，18，4： | 0 |
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