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| 1 | Mitochondrial localization of ORF346 causes pollen abortion in alloplasmic male sterility显示文摘The Nsa cytoplasmic male sterility(CMS) system confers stable male sterility and offers great potential for production of hybrid seeds in oilseed rape. However, genes responsible for male sterility in Nsa CMS have not been identified. By mitochondrial genome sequencing of Nsa CMS and its maintainer line,we identified in an Nsa CMS line several chimeric genes encoding hypothetical proteins harboring transmembrane domains. One novel chimeric gene orf346 showed high identity with cox1 at the 50 terminal region and was co-transcribed with nad3 and rps12 genes. Transgenic plants of orf346 fused with or without mitochondrial targeting peptide conferred complete male sterility in Arabidopsis. ORF346 was mitochondrion-localized. Expression of orf346 in Escherichia coli inhibited bacterial growth, with excessive accumulation of reactive oxygen species and decreased ATP content. These results reveal a link between the newly identified mitochondrial gene orf346 and the abortion of Nsa CMS. Inadequate energy supply and excessive accumulation of reactive oxygen species may account for pollen abortion in Nsa CMS plants. | Shifei Sang Hongtao Cheng Mengyu Hao Bingli Ding Desheng Mei Hui Wang Wenxiang Wang Jia Liu Li Fu Kede Liu Qiong Hu | 2021 | The Crop Journal2021，9，6： | 5 |
| 2 | Kinetics of oxidation of dimethyl trisulfide by potassium permanganate in drinking water显示文摘Metabolites of algae such as geosmin, 2- methylisoborneol etc. are reported to induce pungent odors into drinking water and attract additional scientifc attention. Recently, in China, taste and odor outbreaks in drinking water supply have become increasingly common. In source water affected by eutrophication, dimethyl trisulfide, speculated to be produced by decayed algae, was found to be the source of taste and odor issues and can be removed effectively by usual oxidation agents. In this experimental study, batch scale tests were carried out focusing on the removal of dimethyl trisulfide. Reaction kinetics of dimethyl trisulfide oxidized by potassium permanganate in water had been studied; influence factors such as pH, organic substrate, other existed taste, and odor contaminant in equivalent concentration were also dis- cussed. Results showed that dimethyl trisulfide can be removed by potassium permanganate efficiently; the ratio can reach more than 70% with oxidant dosage of 4 mg. L~ and contact time prolonged to 120 min. The dimethyl trisulfide decomposition followed a second-order kinetics pattern with a rate constant k -- 0.00213 L.(min.mg)-1. Typically, the degradation rate of dimethyl trisulfide was increased with the increasing KMnO4 dosage, but dramatically dropped with the increasing levels of humic acid (1.8-4.5 mg. L-1) and other odor-causing compounds (e.g. fl-cyclocitral, 0-1886.0 pg.L-1). Solution pH (5.2- 9.0) and initial dimethyl trisulfide concentration did not significantly affected the degradation. This study demon- strates that KMnO4 oxidation is an effective option to remove dimethyl trisulfide from water. | Xiaoyan MA Shifei HU Hongyu WANG Jun LI Jing HUANG Yun ZHANG Weigang LU Qingsong LI | 2012 | Frontiers of Environmental Science & Engineering2012，6，2： | 3 |
| 3 | New loci for refractive errors and ocular biometric parameters in young Chinese Han adults显示文摘Myopia has become a major public health issue with an increasing prevalence.There are still individuals who experience similar environmental risk factors and,yet,remain non-myopic.Thus,there might be genetic factors protecting people from myopia.Considering the opposite ocular characteristics of primary angle closure glaucoma(PACG)to myopia and possible common pathway between them,we propose that certain risk genes for PACG might act as a protective factor for myopia.In this study,2,678 young adults were genotyped for 37 targeted single nucleotide polymorphisms.Compared with emmetropia,rs1401999(allele C:OR=0.795,P=0.03;genotype in dominant model:OR=0.759,P=0.02)and rs1258267(allele A:OR=0.824,P=0.03;genotype in dominant model:OR=0.603,P=0.01)were associated with low to moderate myopia and high myopia,respectively.Genotype under recessive model of rs11024102 was correlated with myopia(OR=1.456,P=0.01),low to moderate myopia(OR=1.443,P=0.02)and high myopia(OR=1.453,P=0.02).However,these associations did not survive Bonferroni correction.Moreover,rs1401999,rs1258267,and rs11024102 showed associations with certain ocular biometric parameters in different groups.Our study suggests that ABCC5,CHAT and PLEKHA7 might be associated with refractive errors by contributing to the regulation of ocular biometry,in terms of uncorrected results and their biological functions. | Yunyun Sun Zi-Bing Jin Shifei Wei Hongyan Jia Kai Cao Jianping Hu Caixia Lin Wenzai An Jiyuan Guo He LiJing Fu Shi-Ming Li Ningli Wang the Anyang University Students Eye Study Group the Anyang University Students Eye Study Group | 2022 | Science China(Life Sciences)2022，65，10： | 1 |
| 4 | Ki-netics on oxidation of odor contaminant-dimethyl tri-sulfide by potassium ferrate in drinking water 显示文摘 | Hu Shifei Ma Xiaoyan Wang Hongyu | 2011 | Advanced Materials Research2011，，： | 1 |
| 5 | Complete organelle genomes of Sinapis arvensis and their evolutionary implications显示文摘Sinapis arvensis,belonging to the genus Sinapis of the family Brassicaceae,has good agronomic characters that make it a valuable genetic resource for crop improvement and is a cytoplasmic source of heterologous cytoplasmic male sterility(CMS).In addition,S.arvensis has played an important role in the evolution of the six major cultivated Brassica species involved in the triangle of U.Using next-generation sequencing,we assembled and revealed the gene composition of S.arvensis cytoplasmic genome.The chloroplast genome comprises 153,590 bp,with 112 individual genes,including 4 r RNA,29 t RNA,and 79 proteincoding genes.The mitochondrial genome comprises 240,024 bp,with 54 genes,including 18 t RNA,three r RNA and 33 protein-coding genes.Genome structure and evolutionary analysis indicated that the sequences of the S.arvensis organellar genomes were more similar to those of Brassica nigra and B.carinata than to those of other Brassicaceae species.Four mitochondrial open reading frames displaying chimeric structural features and encoding hypothetical proteins with transmembrane domains may account for the infertility of Nsa CMS previously derived from somatic cell hybridization between B.napus and S.arvensis.These results will not only contribute to utilize the germplasm resource of S.arvensis,and comprehend the evolution of organelle genomes within the Brassicaceae family,but also help to identify genes conditioning the alloplasmic male sterility of Nsa CMS in B.napus. | Shifei Sang Hongtao Cheng Desheng Mei Li Fu Hui Wang Jia Liu Wenxiang Wang Qamar U.Zaman Kede Liu Qiong Hu | 2020 | The Crop Journal2020，8，4： | 1 |
| 6 | Computational Tools and Resources for CRISPR/Cas Genome Editing显示文摘The past decade has witnessed a rapid evolution in identifying more versatile clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein(Cas)nucleases and their functional variants,as well as in developing precise CRISPR/Cas-derived genome editors.The programmable and robust features of the genome editors provide an effective RNAguided platform for fundamental life science research and subsequent applications in diverse scenarios,including biomedical innovation and targeted crop improvement.One of the most essential principles is to guide alterations in genomic sequences or genes in the intended manner without undesired off-target impacts,which strongly depends on the efficiency and specificity of single guide RNA(sgRNA)-directed recognition of targeted DNA sequences.Recent advances in empirical scoring algorithms and machine learning models have facilitated sgRNA design and off-target prediction.In this review,we first briefly introduce the different features of CRISPR/Cas tools that should be taken into consideration to achieve specific purposes.Secondly,we focus on the computer-assisted tools and resources that are widely used in designing sgRNAs and analyzing CRISPR/Cas-induced on-and off-target mutations.Thirdly,we provide insights into the limitations of available computational tools that would help researchers of this field for further optimization.Lastly,we suggest a simple but effective workflow for choosing and applying web-based resources and tools for CRISPR/Cas genome editing. | Chao Li Wen Chu Rafaqat Ali Gill Shifei Sang Yuqin Shi Xuezhi Hu Yuting Yang Qamar U.Zaman Baohong Zhang | 2023 | Genomics, Proteomics & Bioinformatics2023，21，1： | 0 |
| 7 | Establishment of new convenient two-line system for hybrid production by targeting mutation of OPR3 in allopolyploid Brassica napus显示文摘The two-line pollination control system,which usually depends on the utilization of thermosensitive or photoperiod genic male-sterile lines,has been widely used in various crops.However,this system is susceptible to instability issues caused by uncontrollable weather fluctuations.A stable and handy two-line pollination control system is highly desirable in many crop species for heterosis exploitation.Oxophytodienoic acid reductase 3(OPR3)was proven to be involved in jasmonate biosynthesis.In the present study,CRISPR/Cas9(Clustered Regularly Interspaced Short Palindromic Repeat)was utilized to mutate two OPR3 homologs in Brassica napus.After two OPR3 homologs were simultaneously mutated,mutants exhibited complete male sterility,and fertility could be easily restored by exogenous MeJA treatment.Hybrids produced from crosses between the opr3 sterile lines and normal varieties exhibited heterosis.This new two-line system based on OPR3 mutation provides higher stability and convenience than traditional systems.By using exogenous MeJA treatment to restore fertility,the system enables more precise control of male fertility transition,which has great potential to significantly contribute to the maneuverable production of hybrid seeds in rapeseed as well as other Brassica species crops. | Hongtao Cheng Mengyu Hao Shifei Sang Yunfei Wen Yating Cai Hui Wang Wenxiang Wang Desheng Mei Qiong Hu | 2023 | Horticulture Research2023，10，12： | 0 |
| 8 | An efficient approach for obtaining plant organelle genomes显示文摘Plant organelle(plastid and mitochondrial)genomes contain substantial information for plant evolution and adaptation.Therefore,it’s important to reveal plant whole-genome sequences including plastid and mitochondrial genomes.To decode these sequences,it is required to efficiently separate organelle genomic DNA from nucleus genome,which is difficult and laborious.In this study,an efficient procedure was established to obtain plant organelle genomes without extraction of plastid and mitochondria.Organelle DNA was extracted from three materials including Sinapis arvensis var.‘Yeyou 18’,a cytoplasmic male sterile line(Nsa CMS)and its corresponding maintainer line‘Zhongshuang 4’.DNA was sequenced by Roche 454 FLXt and Illumina Miseq platforms.Organelle genomes were assembled using the generated reads and public organelle genome sequences.This research presented a procedure that efficiently assembled organelle genomes and subsequent fill gaps by extending the consensus contig terminals.This method enabled us to assemble plant plastid and mitochondrial genomes simultaneously.The obtained organelle genomes could accelerate understanding of mitochondrial rearrangements and laid a foundation for further study of Sinapis arvensis evolution and sterility gene of Nsa CMS. | Shifei Sang Desheng Mei Qamar U.Zaman Jia Liu Hongtao Cheng Li Fu Wenxiang Wang Hui Wang Qiong Hu | 2020 | Oil Crop Science2020，5，3： | 0 |
| 9 | Distribution of IOP and its relationship with refractive error and other factors:the Anyang University Students Eye Study显示文摘AIM:To investigate the distribution of intraocular pressure(IOP)and its relationship with refractive error and other factors in university students from Anyang,China.METHODS:A university-based study was conducted.Subjects were invited to complete ophthalmic examinations,including visual acuity,noncontact tonometr y(NCT),cycloplegic autorefraction,and ocular biometry.Univariable and multivariable analyses were used to evaluate the associations between IOP and other factors.Only data from right eyes were used in analysis.RESULTS:A total of 7720 subjects aged 16 to 26 years old were included,and 2834(36.4%)of the participants were male.The mean IOP of the right eye for all subjects was 15.52±3.20 mm Hg(95%CI:15.45,15.59).Using multivariate linear regression analysis,IOP was found to correlate significantly with younger age(P<0.001;standardized regression coefficientβ,-0.061;regression coefficientβ,-0.139;95%CI:-0.18,-0.09),higher myopic refractive error(P=0.044;standardizedβ,-0.060;regression coefficientβ,-0.770;95%CI:-0.15,-0.002),higher central corneal thickness(P<0.001;standardizedβ,0.450;regression coefficientβ,0.044;95%CI:0.04,0.05),and shorter axial length(AL;P<0.001;standardizedβ,-0.061;regression coefficientβ,-0.163;95%CI:-0.25,-0.07).CONCLUSION:This study described the normal distribution of IOP.In Chinese university students aged 16-26 y,higherIOP is associated with younger age,higher myopic refractive error,higher thickness of the central cornea,and shorter AL. | Dandan Ma Shifei Wei Yunyun Sun Shi-Ming Li Wen-Zai An Jian-Ping Hu Kai Cao Xiao-Hui Yang Cai-Xia Lin Ji-Yuan Guo He Li Jing Fu Ningli Wang | 2021 | International Journal of Ophthalmology(English edition)2021，14，4： | 0 |
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