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| 1 | Genome analysis of Taraxacum kok-saghyz Rodin provides new insights into rubber biosynthesis显示文摘The Russian dandelion Taraxacum kok-saghyz Rodin(TKS), a member of the Composite family and a potential alternative source of natural rubber(NR) and inulin, is an ideal model system for studying rubber biosynthesis. Here we present the draft genome of TKS, the first assembled NR-producing weed plant. The draft TKS genome assembly has a length of 1.29 Gb, containing 46 731 predicted protein-coding genes and68.56% repeats, in which the LTR-RT elements predominantly contribute to the genome enlargement. We analyzed the heterozygous regions/genes, suggesting its possible involvement in inbreeding depression.Through comparative studies between rubber-producing and non-rubber-producing plants, we found that enzymes of the mevalonate(MVA) pathway and rubber elongation might be critical for rubber biosynthesis, and several key isoforms have been isolated and shown to be predominantly expressed in the latex, indicating their crucial functions in rubber biosynthesis. Moreover, for two important families in rubber elongation, the CPT/CPTL and REF/SRPP families, diverse evolutionary tracks have been revealed. These results provide valuable resources and new insights into the mechanism of NR biosynthesis,and facilitate the development of alternative NR-producing crops. | Tao Lin Xia Xu Jue Ruan Shizhong Liu Shigang Wu Xiujuan Shao Xiaobo Wang Lin Gan Bi Qin Yushuang Yang Zhukuan Cheng Suhua Yang Zhonghua Zhang Guosheng Xiong Sanwen Huang Hong Yu Jiayang Li | 2018 | National Science Review2018，5，1： | 22 |
| 2 | Climate and litter C/N ratio constrain soil organic carbon accumulation显示文摘Soil organic carbon(SOC) plays critical roles in stabilizing atmospheric CO2 concentration, but the mechanistic controls on the amount and distribution of SOC on global scales are not well understood. In turn, this has hampered the ability to model global C budgets and to find measures to mitigate climate change. Here, based on the data from a large field survey campaign with 2600 plots across China’s forest ecosystems and a global collection of published data from forested land, we find that a low litter carbon-to-nitrogen ratio(C/N) and high wetness index(P/PET, precipitation-to-potentialevapotranspiration ratio) are the two factors that promote SOC accumulation, with only minor contributions of litter quantity and soil texture. The field survey data demonstrated that high plant diversity decreased litter C/N and thus indirectly promoted SOC accumulation by increasing the litter quality. We conclude that any changes in plant-community composition, plant-species richness and environmental factors that can reduce the litter C/N ratio, or climatic changes that increase wetness index, may promote SOC accumulation. The study provides a guideline for modeling the carbon cycle of various ecosystem scales and formulates the principle for land-based actions for mitigating the rising atmospheric CO2 concentration. | Guoyi Zhou Shan Xu Philippe Ciais Stefano Manzoni Jingyun Fang Guirui Yu Xuli Tang Ping Zhou Wantong Wang Junhua Yan Gengxu Wang Keping Ma Shenggong Li Sheng Du Shijie Han Youxin Ma Deqiang Zhang Juxiu Liu Shizhong Liu Guowei Chu Qianmei Zhang Yuelin Li Wenjuan Huang Hai Ren Xiankai Lu Xiuzhi Chen | 2019 | National Science Review2019，6，4： | 21 |
| 3 | Effect of long term aging on microstructure and stress rupture properties of a nickel based single crystal superalloy | Zhenxue Shi Jiarong Li Shizhong Liu | 2012 | Progress in Natural Science:Materials International2012，22，5： | 14 |
| 4 | Solidification Simulation of Investment Castings of Single Crystal Hollow Turbine Blade显示文摘The three-dimensional solidification simulation of the investment castings of single crystal hollow turbine blade at the withdrawal rates of 2 mm/min, 4.5 mm/min and 7 mm/min has been performed with the finite element thermal analysis. The calculated results are in accordance with the experimental ones. The results show that with the increase of withdrawal rate the concave curvature of the liquidus isotherm is larger and larger, and the temperature gradients of the blades increase. No effects of withdrawal rate on the distribution of the temperature gradients of the starter and helical grain selector of the blades are observed at withdrawal rates of 2 mm/min, 4.5 mm/min and 7 mm/min. The relatively high temperature gradient between 500℃/cm and 1000℃/cm in the starter and helical grain selector is obtained at three withdrawal rates. | Jiarong LI, Shizhong LIU, Hailong YUAN and Zhengang ZHONGNational Key Laboratory of Advanced High Temperature Structural Materials, Beijing Institute of Aeronautical Materials, Beijing 100095, China | 2003 | Journal of Materials Science & Technology2003，19，6： | 9 |
| 5 | Effects of Rhenium on Creep Rupture Life of a Single Crystal Superalloys显示文摘The slabs of a single crystal superalloy were poured with 2.2% Re, 3.2% Re and 4.2% Re and otherelement contents of the alloys were basicalIy kept no changed. The effects of Re on the ultimatetensile strength, creep rupture life and rafting of a single crystal superalloy were studied. Theresults showed that with increasing Re content the ultimate tensile strength decreased but theplasticity increased at 649℃. and the creep rupture life remarkably increased while the plasticitydecreased at 982℃/248.2 MPa. 1038℃/206.8 MPa and 1070℃/176.8 MPa for the alloy, on themicrostructure aspect, with increasing the creep rupture life of the alloy the rafting was gettingbigger and bigger at the same test condition, and the rafting suppressed creep and increasedcreep rupture life in this single crystal superalloy. | Li, Jiarong Tang, Dingzhong Lao, Riling Liu, Shizhong Wu, Zhongtang
| 1999 | Journal of Materials Science & Technology1999，15，1： | 8 |
| 6 | Solidification Simulation of Single Crystal Investment Castings显示文摘The three dimensional solidification simulation of the single crystal investment castings at withdrawal rates of 2 mm/min, 4.5 mm/min and 7 mm/min was performed with the finite element thermal analysis method. The calculated results were in accordance with the experimental ones. The results showed that with the increase of withdrawal rate the concave curvature of the liquidus isotherm was bigger and bigger and the temperature gradient of the castings decreased. No effects of withdrawal rate on the distribution of the temperature gradient of the starter and helical grain selector of the castings were observed at withdrawal rates of 2 mm/min, 4.5 mm/min and 7mm/min.The relatively high temperature gradient between 500°C/cm and 1000°C/cm in the starter and helical grain selector was obtained at three withdrawal rates. The study indicates the three dimensional solidification simulation by finite element method is a powerful tool for understanding solidification and predicting defects in single crystal investment castings. | Jiarong LI, Shizhong LIU and Zhengang ZHONGBeijing Institute of Aeronautical Materials, Beijing 100095, China | 2002 | Journal of Materials Science & Technology2002，18，4： | 7 |
| 7 | Chimeric flagellin expressed by Salmonella typhimurium induces an ESAT-6-specific Th1-type immune response and CTL effects following intranasal immunization显示文摘The flagellin component FliC of Salmonella typhimurium is capable of activating the innate immune system via specific interactions with TLR5 and can also act as a carrier of foreign antigen to elicit antigen-specific immune responses.Thus,we constructed an attenuated Salmonella strain SL5928(fliC/esat)expressing chimeric flagellin that contained the ESAT-6 antigen coding sequence of Mycobacterium tuberculosis inserted into the highly variable region of the Salmonella flagellin coding gene fliCi.The chimeric flagellin functioned normally,as demonstrated using a flagella swarming assay and electron microscopy.To analyze the effects of chimeric flagellin,the cell-mediated immune response and cytotoxic T lymphocyte(CTL)effects specific for ESAT-6 antigen were tested after intranasal immunization of mice with flagellated Salmonella SL5928(fliC/esat).The results showed that SL5928(fliC/esat)intranasal immunization can strongly elicit an ESAT-6-specific T helper(Th)1-type immune response in mucosal lymphoid tissues,such as nasopharynx-associated lymph nodes,lung and Peyer’s patches,and a Th1/Th2 response was elicited in spleen and mesenteric lymph nodes.Furthermore,intranasal immunization of SL5928(fliC/esat)produced efficient CTL effects,as demonstrated using a 5-and 6-carboxyfluorescein diacetate succinimidyl ester(CFSE)assay.Thus,our study revealed that Salmonella flagellin acts as a carrier for foreign antigen and triggers strong Th1 and CTL responses during intranasal immunization.Chimeric flagellin is potentially an effective strategy for the development of novel vaccines against tuberculosis in humans and animals. | Hui Zhang Liu Liu Ke Wen Jinlin Huang Shizhong Geng Junsong Shen Zhiming Pan Xinan Jiao | 2011 | Cellular & Molecular Immunology2011，8，6： | 6 |
| 8 | CO_2 efflux from different forest soils and impact factors in Dinghu Mountain, China显示文摘CO2 fluxes from soils and related environmental factors were measured in three forest ecosystems of Dinghu Mountain using static chamber-gas chromatograph technique for one year. The seasonal pattern of CO2 flux, contribution of litter on total CO2 flux and the correlations of CO2 flux with soil temperature and soil water content were examined for each type of forest. The results were given as followings: (1) The seasonal patterns of CO2 flux from soil of the three types of forest were similar, with a higher CO2 flux in rainy season than in dry season. The comparative relations of mean annual CO2 fluxes between the three sites were expressed as:monsoon forest > mixed forest > pine forest. (2) CO2 fluxes from litter decomposition in monsoon forest, mixed forest and pine forest accounted for 24.43%, 41.75% and 29.23% of the corresponding total CO2 fluxes from forest floor, respectively. (3) Significant relationships were found between CO2 fluxes and soil temperatures at 5 cm depth for the three types of forest, which could be best described by exponential equations. The calculated Q10 values based on soil temperature at 5 cm depth ranged from 1.86 to 3.24. More significant relationships were found between CO2 fluxes and soil water content when the annual variation coefficients of soil moisture were higher. | ZHOU Cunyu, ZHOU Guoyi, ZHANG Deqiang, WANG Yinghong & LIU Shizhong Dinghushan Forest Ecosystem Research Station, Chinese Academy of Sciences, Guangzhou 510650, China Institute of Atmospheric Physics, Chinese Academy of Sciences, Beijing 100029, China | 2005 | Science China Earth Sciences2005，48，z1： | 6 |
| 9 | Kernel selection with spectral perturbation stability of kernel matrix显示文摘Kernel selection is one of the key issues both in recent research and application of kernel methods.This is usually done by minimizing either an estimate of generalization error or some other related performance measure. Use of notions of stability to estimate the generalization error has attracted much attention in recent years. Unfortunately, the existing notions of stability, proposed to derive the theoretical generalization error bounds, are difficult to be used for kernel selection in practice. It is well known that the kernel matrix contains most of the information needed by kernel methods, and the eigenvalues play an important role in the kernel matrix. Therefore, we aim at introducing a new notion of stability, called the spectral perturbation stability,to study the kernel selection problem. This proposed stability quantifies the spectral perturbation of the kernel matrix with respect to the changes in the training set. We establish the connection between the spectral perturbation stability and the generalization error. By minimizing the derived generalization error bound, we propose a new kernel selection criterion that can guarantee good generalization properties. In our criterion,the perturbation of the eigenvalues of the kernel matrix is efficiently computed by solving the derivative of a newly defined generalized kernel matrix. Both theoretical analysis and experimental results demonstrate that our criterion is sound and effective. | LIU Yong LIAO ShiZhong | 2014 | Science China(Information Sciences)2014，57，11： | 5 |
| 10 | Effects of precipitation on soil organic carbon fractions in three subtropical forests in southern China显示文摘Aims The aim of this study was to investigate the effects of precipitation changes on soil organic carbon(SOC)fractions in subtropical forests where the precipitation pattern has been altered for decades.Methods We conducted field manipulations of precipitation,including ambient precipitation as a control(CK),double precipitation(DP)and no precipitation(NP),for 3 years in three forests with different stand ages(broadleaf forest[BF],mixed forest[MF]and pine forest[PF])in subtropical China.At the end of the experiment,soil samples were collected to assay SOC content,readily oxidizable organic carbon(ROC)and non-readily oxidizable organic carbon(NROC),as well as soil microbial biomass carbon(MBC),pH and total nitrogen content.Samples from the forest floors were also collected to analyze carbon(C)functional groups(i.e.alkyl C,aromatic C,O-alkyl C and carbonyl C).Furthermore,fine root biomass was measured periodically throughout the experiment.Important Findings Among the forests,ROC content did not exhibit any notable differences,while NROC content increased significantly with the stand age.This finding implied that the SOC accumulation observed in these forests resulted from the accumulation of NROC in the soil,a mechanism for SOC accumulation in the mature forests of southern China.Moreover,NP treatment led to significant reductions in both ROC and NROC content and therefore reduced the total SOC content in all of the studied forests.Such decreases may be due to the lower plant-derived C inputs(C quantity)and to the changes in SOC components(C quality)indicated by C functional groups analyses under NP treatment.DP treatment in all the forests also tended to decrease the SOC content,although the decreases were not statistically significant with the exception of SOC and ROC content in PF.This finding indicated that soils in MF and in BF may be more resistant to precipitation increases,possibly due to less water limitations under natural conditions in the two forests.Our results therefore highlight the different responses of SOC and its fractions to precipitation changes among the forests and suggest that further studies are needed to improve our understanding of SOC dynamics in such an important C sink region. | Xiaomei Chen Deqiang Zhang Guohua Liang Qingyan Qiu Juxiu Liu Guoyi Zhou Shizhong Liu Guowei Chu Junhua Yan | 2016 | Journal of Plant Ecology2016，9，1： | 4 |
| 11 | Chromosome-scale genome assembly of sweet cherry(Prunus avium L.)cv.Tieton obtained using long-read and Hi-C sequencing显示文摘Sweet cherry(Prunus avium)is an economically significant fruit species in the genus Prunus.However,in contrast to other important fruit trees in this genus,only one draft genome assembly is available for sweet cherry,which was assembled using only Illumina short-read sequences.The incompleteness and low quality of the current sweet cherry draft genome limit its use in genetic and genomic studies.A high-quality chromosome-scale sweet cherry reference genome assembly is therefore needed.A total of 65.05 Gb of Oxford Nanopore long reads and 46.24 Gb of Illumina short reads were generated,representing~190x and 136x coverage,respectively,of the sweet cherry genome.The final de novo assembly resulted in a phased haplotype assembly of 344.29 Mb with a contig N50 of 3.25 Mb.Hi-C scaffolding of the genome resulted in eight pseudochromosomes containing 99.59%of the bases in the assembled genome.Genome annotation revealed that more than half of the genome(59.40%)was composed of repetitive sequences,and 40,338 protein-coding genes were predicted,75.40%of which were functionally annotated.With the chromosomescale assembly,we revealed that gene duplication events contributed to the expansion of gene families for salicylic acid/jasmonic acid carboxyl methyltransferase and ankyrin repeat-containing proteins in the genome of sweet cherry.Four auxin-responsive genes(two GH3s and two SAURs)were induced in the late stage of fruit development,indicating that auxin is crucial for the sweet cherry ripening process.In addition,772 resistance genes were identified and functionally predicted in the sweet cherry genome.The high-quality genome assembly of sweet cherry obtained in this study will provide valuable genomic resources for sweet cherry improvement and molecular breeding. | Jiawei Wang Weizhen Liu Dongzi Zhu Po Hong Shizhong Zhang Shijun Xiao Yue Tan Xin Chen Li Xu Xiaojuan Zong Lisi Zhang Hairong Wei Xiaohui Yuan Qingzhong Liu | 2020 | Horticulture Research2020，7，1： | 4 |
| 12 | Different surface modification methods and coating materials of zinc metal anode显示文摘Rechargeable aqueous Zn-ion batteries(AZIBs)are one of the most promising energy storage devices for large-scale energy storage owing to their high specific capacity,eco-friendliness,low cost and high safety.Nevertheless,zinc metal anodes suffer from severe dendrite growth and side reactions,resulting in the inferior electrochemical performance of AZIBs.To address these problems,surface modification of zinc metal anodes is a facile and effective method to regulate the interaction between the zinc anode and an electrolyte.In this review,the current challenges and strategies for zinc metal anodes are presented.Furthermore,recent advances in surface modification strategies to improve their electrochemical performance are concluded and discussed.Finally,challenges and prospects for future development of zinc metal anodes are proposed.We hope this review will be useful for designing and fabricating highperformance AZIBs and boosting their practical applications. | Feng Tao Yong Liu Xinyuan Ren Jing Wang Yazhou Zhou Yingjie Miao Fengzhang Ren Shizhong Wei Jianmin Ma | 2022 | Journal of Energy Chemistry2022，31，3： | 4 |
| 13 | Effect of the microbial lipopeptide on tumor cell lines: apoptosis induced by disturbing the fatty acid composition of cell membrane显示文摘Microbial lipopeptides play an important role in apoptosis induction of tumor cells.However,there is little knowledge about the relationship between apoptosis induction and membrane fatty acids.The present study focused on the effects of lipopeptides produced by Bacillus subtilis HSO121 on Bcap-37 cell lines.3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl(MTT)colorimetric assay and surface tension measurements,showed that the critical micelle concentration(CMC)was a critical level for the inhibitory activity of lipopeptides on the growth of Bcap-37 cells.Under the CMC,the order of least to greatest cytotoxicity effect on cancer cell lines by lipopeptides is C13-lipopeptide | Xiangyang Liu Xinyi Tao Aihua Zou Shizhong Yang Lixin Zhang Bozhong Mu | 2010 | Protein & Cell2010，1，6： | 3 |
| 14 | Optical bandgap energy of CH3NH3PbI3 perovskite studied by photoconductivity and reflectance spectroscopy显示文摘Organic-inorganic hybrid perovskite materials have been receiving considerable attention due to their promising applications in many optoelectronic fields. However, the optical bandgap of CH_3NH_3PbI_3 perovskite is still disputed. In order to comprehend the intrinsic characteristics of these materials, we study the near-band-edge optical responses of the solution-processed CH_3NH_3PbI_3 perovskite crystals by photoconductivity and reflectance spectroscopy at room temperature. All these spectra of the CH_3NH_3PbI_3 polycrystals show only one significant absorption edge at 1.58 eV. However, we observe an extra absorption edge at 1.47 eV in CH_3NH_3PbI_3 single crystal. We establish a simple kinetic model of charge annihilation processes to explain why the low-energy structure of the photoconductivity and diffuse reflectance spectra of CH_3NH_3PbI_3 polycrystals disappear, which provides another possibility for understanding the difference in absorption edge. It is noteworthy that this low-energy absorption edge of CH_3NH_3PbI_3 single crystal has, to the best of our knowledge, seldom been reported on organic-inorganic hybrid perovskites.Therefore, the CH_3NH_3PbI_3 single crystal exhibits a relatively wide absorption which makes it a promising candidate for photoelectric applications. | HUANG Wei LIU Yu YUE ShiZhong ZHU LaiPan JIN Peng WU Qing ZHANG Yang QU ShengChun WANG ZhiJie CHEN YongHai | 2018 | Science China(Technological Sciences)2018，61，6： | 3 |
| 15 | Comprehensive test stand for high-intensity cyclotron development显示文摘A comprehensive test stand for high-intensity cyclotron development,a 10 MeV 430 μA cyclotron,has been successfully constructed at China Institute of Atomic Energy (CIAE) for the experimental verification of the viability of a 100 MeV high-intensity cyclotron (CYCIAE-100) being constructed at CIAE.The verification includes the overall design technology,detail design and engineering technology for the crucial parts of the machine,e.g.main magnet and beam diagnostics.It will also pave the way for future enhancement of the beam intensity.The test stand consists of a H-ion source,axial injection system,central region,main magnet and coil,RF resonator,stripping extraction,and auxiliary systems,which include the RF power amplifier,high voltage and DC power supplies,electric apparatus,beam diagnostics,control,vacuum,water cooling,pneumatic system,and safety interlock.This paper presents the work regarding the design and sub-system development of the test sand,and the results of the beam commissioning of the machine. | ZHANG TianJue LI ZhenGuo CHU ChengJie XING JianSheng GUAN FengPing ZHONG JunQing JI Bin GE Tao YIN ZhiGuo HOU ShiGang PAN GaoFeng YAO HongJuan LU YinLong WANG ZhenHui WU LongCheng LIN Jun JIA XianLu WEI SuMin WEN LiPeng WANG Feng XIA Le CAI HongRu XIE HuaiDong CHEN RongFan ZHANG Yan ZHANG SuPing LIU GengShou ZOU Jian AN ShiZhong YANG JianJun BI YuanJie YANG Fang | 2011 | Chinese Science Bulletin2011，56，3： | 3 |
| 16 | Low cycle fatigue properties and microstructure evolution at 760℃ of a single crystal superalloy显示文摘Low cycle fatigue(LCF) behavior of a single crystal superalloy was investigated at 760 ℃. Microstructure evolution and fracture mechanism were studied by scanning electron microscopy(SEM) and transmission electron microscopy(TEM), respectively. The results show that the fatigue data fluctuation was small and the fatigue parameters of the alloy had been determined. On increasing the cyclic number, the alloy initially showed slight cyclic softening at the early two or three cycles and slowly hardened to some extent afterwards, then kept stable for the most of the remaining fatigue life. The LCF of the alloy at 760 ℃ can be attributed to the main elastic damage in fatigue processing. The initiation site of fatigue crack was at or near the surface of the samples. Crack propagated perpendicularly to the loading direction at first and then along {111}octahedral slip planes. The fatigue fracture mechanism was quasi-cleavage fracture. The γ'phase morphology still maintained cubic shape after fracture. There were a number of slip bands shear the γ'precipitates and γ matrix near the fracture surface of the specimen. The inhomogeneous deformation microstructure was developed by dislocation motion of cross-slip and a limited γ'precipitate shearing by slip band, stacking faults or single dislocation was observed. | Zhenxue Shi Xiaoguang Wang Shizhong Liu Jiarong Li | 2015 | Progress in Natural Science:Materials International2015，25，1： | 2 |
| 17 | Comparative study of video-assisted thoracoscopic surgery ablation and radiofrequency catheter ablation on treating paroxysmal atrial fibrillation: a randomized, controlled short-term trial显示文摘 | Wang Shizhong Liu Liqun Zou Chengwei | 2014 | Chinese Medical Journal2014，，14： | 2 |
| 18 | A Reconfigurable Microstrip Patch Antenna with Frequency and Circular Polarization Diversities显示文摘A novel microstrip patch antenna with both frequency and circular polarization diversities is presented. The proposed antenna consists of two parts, a patch with two orthogonal slots on the upper substrate and a3 d B hybrid coupler fed structure which printed on the lower substrate. Two groups of diodes placed across the orthogonal slots are utilized to achieve frequency reconfiguration. Another two diodes, placed between the input port of the antenna and the 3d B hybrid coupler, are utilized to switch between Left-hand circular polarization(LHCP)and Right-hand circular polarization(RHCP). Full wave simulation is used for the antenna analysis, and a prototype of the antenna with an integrated Direct current(DC) biasing circuit has been fabricated and measured. The results verify the reconfigurable characteristics of the antenna and demonstrate a good candidate for wireless communication applications. | ZHANG Pengfei LIU Shizhong CHEN Rongrong HUANG Xinglin | 2016 | Chinese Journal of Electronics2016，25，2： | 2 |
| 19 | China’s bioenergy industry development roadmap显示文摘Positive development of renewable energy, saving and substitution of fossil energy, promotion of the energy structure adjustment are the inevitable strategy choices of China’s sustainable development. This paper discussed the China’s bioenergy resources status, development targets and technology development roadmaps. China has 136.140 million hm2 of marginal land, which distribute mainly in western and northern regions. There are 1 billion t of crop residues and forestry waste annually, and 300 million t can be used to produce different kinds of bioenergies. And organic waste and manure can generate 50 billion m3 of biogas. The discussed development target indicated that it can construct a biomass oilfield with the capacity of 100 million t/year and reduce 200 million t of CO_2 emission by 2020. The bioenergy technology development roadmap indicated that the bioethanol mainly uses non grain starch and hemicellulose products as raw materials in the near-term (2006-2010). The biodiesel technology will focus on the advanced production technology, FT diesel, liquefaction of biomass and raw material production technology. | Shi Yuanchun Li Shizhong Liu Xuejun | 2009 | Engineering Sciences2009，7，2： | 2 |
| 20 | Dll4-Notch signaling in regulation of tumor angiogenesis显示文摘 | Zhaoguo Liu Fangtian Fan Aiyun Wang Shizhong Zheng Yin Lu | 2014 | Journal of Cancer Research and Clinical Oncology2014，，4： | 1 |
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