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| 1 | Diversity and high nitrogenase activity of endophytic diazotrophs isolated from Oryza rufipogon Griff.显示文摘Diversity and nitrogenase activity of endophytic diazotrophs colonized in the wild rice Oryza rufipogon Griff grown in Boluo, Huilai County in Guangdong Province and Lingshui County in Hainan Province were studied. Thirty-seven isolates obtained from Oryza rufipogon were identified as putative endophytic nitrogen-fixing bacteria by ARA (acetylene reduction assay) test and further confirmed by PCR amplification of nifH gene fragments. All obtained strains have ARA activity and the same sized nifH gene fragments. Above the similarity level of 80%, the obtained isolates were assigned as Group Ⅰ to Ⅷ by the clustering of IS-PCR fingerprints. The SDS-PAGE whole-cell protein patterns were similar to those of IS-PCR fingerprints. Components and contents of fatty acid methyl esters (FAMEs) were used to differentiate the representative strains (Ls13, Ls8, BL1, BL12, HL6, Ls4) from Group Ⅰ to Group Ⅵ. The six representative strains showed significant difference in contents and components of cellular fatty acid methyl ester. 16S rDNA sequencing analysis showed that strains of Group Ⅰ to Ⅶ were located in Enterobacteraceae (γ-proteobacteria). Strains of Group Ⅰ and Group II were closely related to Klebsiella sp.; Strain Ls8 of Group Ⅱ was a little far away from the genus of Pantoea (homology level 96% with Pantoea agglomerans), which may represent a new species or genus in Enterobacteraceae; Strains of Groups IV and V belonged to different Enterobacter sp.; Strain Ls4 and Ls 9 representing Group Ⅵ were close to Citrobacter amalonaticus with 98% sequence similarity; Strain Ls15 of Group Ⅶ showed 98% sequence identity with Pantoea sp.; Strains of Group Ⅷ were assigned to the genus Ideonella (β-proteobacteria). Based on the above results, endophytic diazotrophs isolated from O. rufipogon showed great diversity and some diazotrophs showed high nitrogenase activity with 42.52 μmol/mL·h C2H4. Inoculation to rice tests indicated that the isolated endophytic diazotrophs significantly promoted the rice growth. | TAN ZhiYuan PENG GuiXiang XU PeiZhi AI ShaoYing TANG ShuanHu ZHANG GuoXia ZENG FengYun | 2009 | Chinese Science Bulletin2009，54，16： | 14 |
| 2 | EFFECT OF TEMPERATURE-DEPENDENT PROPERTIES ON THERMOELASTIC PROBLEMS WITH THERMAL RELAXATIONS显示文摘Based on the generalized thermoelasticity proposed by Green and Lindsay, the dynamic response of generalized thermoelastic problems with temperature-dependent material properties is investigated. The governing equations are formulated and found to be nonlinear because of the temperature-dependence of properties. Owing to the nonlinearity of the governing equations, the finite element method is resorted to for solution. The results obtained show that the temperature-dependent properties influence the variables considered by reducing their magnitudes. This indicates that taking the temperature-dependence of properties into consideration in the investigation of generalized thermoelastic problems is necessary and practical for accurately predicting the thermoelastic behavior. | Tianhu He Shuanhu Shi | 2014 | Acta Mechanica Solida Sinica2014，27，4： | 5 |
| 3 | Analysis of Measures and Effect on Reducing Olefin Content in Gasoline显示文摘This article refers to major measures for reducing olefin content of automotive gasoline and the effect after adoption of these measures. The key for reducing olefin content in China's automotive gasoline pool is to reduce the olefin content of FCC naphtha. The domestic refiners apply the olefinreducing catalyst to decrease the olefin content of FCC gasoline as a convenient and cheap means to meet the national standard for automotive gasoline at the present phase. Furthermore, the novel domestic FCC reaction processes, such as the MIP, MGD, FDFCC and other processes can also apparently reduce olefin content in FCC gasoline. In order to further reduce the olefin content in gasoline to meet more stringent standard for automotive gasoline, Chinese refiners should optimize the processing scheme while aggressively disseminating hydrogenation process along with development of catalytic reforming,alkylation, etherification and other processes to completely change the simplistic composition of domestic gasoline pool. | Qi Hongxiang Kou Shuanhu Dai Zhenyu | 2005 | China Petroleum Processing & Petrochemical Technology2005，7，2： | 2 |
| 4 | Advances of radical and photo reactions in natural products synthesis显示文摘This review will focus on the recent advances of radical and photo reactions and their applications in the natural products total synthesis. A brief introduction to the development and mechanism of the newly developed radical and photo reactions will be presented. The organization of the each section is based on the type of reactions used. Examples of synthetic applications are discussed to demonstrate the potential of related methodologies and inspire future explorations. | Shuanhu Gao Yuanyou Qiu | 2016 | Science China Chemistry2016，59，9： | 2 |
| 5 | Cluster Analysis of Gene Expression Data Based on Self-splitting and Merging Competitive Learning显示文摘 | Shuanhu Wu Alan Wee-Chung Liew Hong Yan | 2004 | IEEE Trans on Information Technology in Biomedicine2004，8，1： | 1 |
| 6 | Evolution of microbial community diversity and enzymatic activity during composting显示文摘 | Yumei He Kaizhi Xie Peizhi Xu Xu Huang Wenjie Gu Fabao Zhang Shuanhu Tang | 2012 | Research in Microbiology2012，，： | 1 |
| 7 | Estrogen Modulates Estrogen Receptor a and b Expression, Osteogenic Activity,and Apoptosis in Mesenchymal Stem Cells (MSCs) of Osteoporotic Mice 显示文摘 | Shuanhu Z Yoram Z Karsten W | 2001 | Cellular Biochemistry Supplement2001，36，： | 1 |
| 8 | Demineralized bone promotes chondrocyte or osteoblast differentiation of human marrow stromal cells cultured in collagen sponges显示文摘 | Shuanhu Z Karen E | 2005 | Cell and Tissue Banking2005，6，2： | 1 |
| 9 | Eukaryotic promoter prediction based on relative entropy and positional information 显示文摘 | Wu Shuanhu Xie Xudong Wee A | 2007 | Physical Review E2007，75，04： | 1 |
| 10 | Cloning and Expression of the Gene Encoding an Extracellular Alkaline Serine Protease from Vibrio Alginolyticus Strain HY9901, the Causative Agent of Vibriosis in Lutjanus Erythopterus (Bloch)显示文摘 | CAI Shuanhu WU Zhaohe JIAN Jichang | 2007 | Journal of Fish Diseases2007，30，8： | 1 |
| 11 | Eukaryotic promoter prediction based on relative entropy and positional information显示文摘 | Shuanhu Wu Xudong Xie | 2007 | Physical Review E2007，75，04： | 1 |
| 12 | Vitamin D metabo- lism in human bone marrow stromal (mesenchymal stem) cells显示文摘 | Shuo Geng Shuanhu Zhou Zhenggang Bi | 2013 | Metabolism2013，62，6： | 1 |
| 13 | Clinical char- acteristics influence in vitro action of 1,25 - dihydroxyvitamin D (3) in human marrow stromal cells 显示文摘 | Shuanhu Zhou Julie Glowacki Sung Won Kim | 2012 | Journal of Bone and Min- eral Research2012，27，9： | 1 |
| 14 | Effects of 25 - hydroxyvitamin D (3) on proliferation and osteoblast differentiation of human marrow stromal cells require CYP27B1/1 alpha - hydrox- ylase 显示文摘 | Shuo Geng Shuanhu Zhou Julie Glowacki | 2011 | Journal of Bone and Mineral Research2011，26，5： | 1 |
| 15 | Age -related decline in osteoblastogenesis and lalpha- hydroxylase/CYP27Bl in human mesenchymal stem cells : stimulation by parathyroid hormone 显示文摘 | Shuo Geng Shuanhu Zhou Julie Glowacki | 2011 | Aging Cell2011，10，6： | 1 |
| 16 | Movement characteristics and present seismic activity of Ordos Block显示文摘The present horizontal movement of the Ordos Block is the result of the movement interaction between the Ordos Block and the mainland of China.The main dynamic sources are the northeastern pushing by Qinghaie Tibet Block,and the northwestern extension of the northern margin of the block.The latest GPS velocity field observational evidences shows that the velocity field of Ordos Block and around has a significant change from 1999 to 2015,and the change is significant in the northeast margin,the northwest margin,the southwest margin,and the southeast margin of the block.The velocity of left lateral movement of the northsouthern margin of Ordos Block is much larger than the right lateral movement of its eastsouthern margin.By using the seismic data of Ordos Block since 1970,the analysis of strain accumulation,strain release,stress distribution,and stress field show that the northern margin of Ordos Block is the main active area not only in the block edge,but also in North China.The strain around Ordos Block has been accumulating continuously since 2000,and this is the longest period of strain accumulation since 1970.Some signs of strain relief appeared after Alashanzuoqi Ms5.8 earthquake in 2015.There are some areas with extremely large strain anomalies appear in the northern margin of Ordos Block. | Lixin Gao Di Hou Juan Li Shuanhu Li Yong Dai Feng Xiong Hongying Yang | 2016 | Geodesy and Geodynamics2016，7，6： | 1 |
| 17 | Estrogen modulates Estrogen Receptor a and b Expression,Osteogenic Activity and Apoptosis in mesenchymal stem cells(MSCs)of Osteoporotic Mice显示文摘 | Shuanhu Z Yoram Z Karsten W | 2001 | Cellular Biochemistry Supplement2001，36，： | 1 |
| 18 | Biomimetic Synthesis of Equisetin and (+)‐Fusarisetin A显示文摘 | Jun Yin Lili Kong Cheng Wang Yingbo Shi Shujun Cai Shuanhu Gao | 2013 | Chem. Eur. J2013，，39： | 1 |
| 19 | Total Synthesis of Three Families of Natural Antibiotics:Anthrabenzoxocinones,Fasamycins/Naphthacemycins,and Benastatins显示文摘Due to the critical bacterial resistance and antibiotic crisis,the discovery of new antibiotics is an urgent need in the clinic than ever.Naturally occurring antibiotics have proven to be an indispensable source of the development of new antibacterial agents.Herein,we report the total synthesis of three families of biogenetically related natural antibiotics,including anthrabenzoxocinones(ABXs),fasamycins/naphthacemycins,and benastatins.The synthesis featured divergent and convergent approaches,which enabled efficient construction of the basic polycyclic skeletons in 6–10 steps on a large-scale,followed by a collective synthesis of 14 natural products and their corresponding analogs.The core scaffold of gemdimethyl-anthracenone,a naturally occurring type II fatty acid-specific condensation enzyme(FabFspecific)antibiotic pharmacophore,was forged via a Ti(Oi-Pr)4-mediated photoenolization/Diels–Alder(PEDA)reaction between 2-isopropyl benzaldehyde and a variety of enones.A scale-up of the PEDA reaction was facilitated in an assembled continuous-flow reactor,which allowed us to overcome the issues associated with batch photochemistry.Subsequently,the synthetic natural antibiotics and their analogs would be utilized in structure–activity relationships(SAR)and mechanism studies,which should enable the discovery of new and leading antibiotic compounds. | Delu Jiang Kunyun Xin Baochao Yang Yanyu Chen Quan Zhang Haibing He Shuanhu Gao | 2020 | CCS Chemistry2020，2，6： | 1 |
| 20 | A general and efficient strategy for 7-aryloctahydroindole and cis-3a-aryloctahydroindole alkaloids: Total syntheses of ( + / -)-gamma-lycorane and ( +/- )-crinane 显示文摘 | Gao Shuanhu Tu Yongqiang Song Zhenlei | 2005 | J OrgChem2005，70，16： | 1 |
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