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| 1 | Cardiac autonomic nerve fiber regeneration in chronic heart failure Do Akt gene-transduced mesenchymal stem cells promote repair?显示文摘BACKGROUND: Transplantation of Akt-over-expressing mesenchymal stem cells (Akt-MSCs) has been shown to repair infarcted myocardium and improve cardiac function. However, little is known about the therapeutic effects of Akt-MSCs on cardiac autonomic neuropathy in chronic heart failure (CHF). OBJECTIVE: The present study used adriamycin-induced CHF rat models to observe the effect of Akt-MSCs on cardiac autonomic nervous regeneration and the factors mediating this effect. DESIGN, TIME AND SETTING: A randomized, controlled animal experiment was performed at the Central Laboratory of Basic Medical College, China Medical University, between September 2008 and April 2009. MATERIALS: Rabbit anti-choline acetyltransferase (ChAT), growth associated protein-43 (GAP-43), synaptophysin (SYN) polyclonal antibodies and the secondary antibody (goat anti-rabbit IgG) were purchased from Boster, China. Cat-A-Kit assay system was provided by Amersham, USA. METHODS: (1) Adult rat MSCs were isolated and cultured for the preparation of Akt-MSCs. (2) Forty male Wistar rats were intramyocardially administered adriamycin at 2 mg/kg over 3 days for a total of five times and once a week for additional five times thereafter to establish CHF models. At 2 weeks after final adriamycin treatment, 34 successful CHF rat models were randomized to three groups: Akt-MSCs (n = 11), simple MSCs (s-MSCs, n =11), and control (n = 12). Each group was intravenously administered Akt-MSCs (2×106 cells in 100 μL PBS), s-MSCs (2×106 cells in 100 μL PBS) or equal volume of phosphate buffered saline, once a day for a total of three times. MAIN OUTCOME MEASURES: At 4 weeks after final adriamycin treatment, myocardial norepi- nephrine (NE) content was detected using a Cat-A-Kit assay system. Myocardial ChAT, SYN and GAP-43 were performed by immunohistochemistry and Western blot analysis. Prior to, 2 and 4 weeks after adriamycin treatment, echocardiographic examination was performed and left ventricular ejection fraction (LVEF) was determined. RESULTS: Myocardial NE content, as well as SYN-positive and GAP-43-positive nerve fiber density and expression, and LVEF, was the greatest in the Akt-MSCs group, followed by the s-MSCs group, and lastly the control group (P < 0.05 or P < 0.01). ChAT expression was similar between Akt-MSCs and s-MSCs groups, but it was higher compared with the control group (P < 0.05). NE contents were negatively correlated to LVEF (r = -0.64, P = 0.015). CONCLUSION: Transplantation of MSCs, in particular Akt-MSCs, promotes cardiac nervous regeneration in failing heart, which might be mediated by GAP-43. | Hongliang Kong Zhanquan Li Shumei Zhao Li Zhu Yingjun Zhao Weiwei Zhang GuipingXu Wenjun Hao Huijun Li Guoxian Qi | 2010 | Neural Regeneration Research2010，5，1： | 13 |
| 2 | High quality reference genome of drumstick tree(Moringa oleifera Lam.), a potential perennial crop显示文摘The drumstick tree(Moringa oleifera Lam.) is a perennial crop that has gained popularity in certain developing countries for its high-nutrition content and adaptability to arid and semi-arid environments. Here we report a high-quality draft genome sequence of M. oleifera. This assembly represents 91.78% of the estimated genome size and contains 19,465 protein-coding genes. Comparative genomic analysis between M. oleifera and related woody plant genomes helps clarify the general evolution of this species, while the identification of several species-specific gene families and positively selected genes in M. oleifera may help identify genes related to M. oleifera's high protein content, fast-growth, heat and stress tolerance. This reference genome greatly extends the basic research on M. oleifera, and may further promote applying genomics to enhanced breeding and improvement of M. oleifera. | TIAN Yang ZENG Yan ZHANG Jing YANG Cheng Guang YAN Liang WANG XuanJun SHI ChongYing XIE Jing DAI TianYi PENG Lei ZENG HUAN Yu XU An Ni HUANG YeWei ZHANG JiaJin MA Xiao DONG Yang HAO ShuMei SHENG Jun | 2015 | Science China(Life Sciences)2015，58，7： | 10 |
| 3 | Genome of Plant Maca (Lepidium meyenil) Illuminates Genomic Basis for High-Altitude Adaptation in the Central Andes显示文摘Maca (Lepidium meyenii Walp, 2n = 8x = 64 ) ,属于 Brassicaceae 家庭,经济植物在秘鲁(4000-4500 m ) 在中央安第斯山脉岭被栽培。认为快速中央安第斯山脉高举发生 5-10 百万年以前(妈) ,一个进化问题关于象 maca 那样的植物怎么在一个短地质的时期以内获得高高度的改编产生。这里,我们报导 maca 的高质量的染色体集会,在里面它二仔细 spaced maca 特定的整个染色体的复制(WGD;6.7 妈) 被识别。在 maca 和密切相关的 Brassicaceae 种类之间的比较 genomic 分析揭示了经由 WGD 涉及不能生活的压力反应,荷尔蒙发信号小径,和第二等的代谢物生合成的 maca 基因和基因家庭的扩大。保留和许多复制基因的随后的功能的分叉可以说明词法、生理的变化(即,小叶形状和自我富饶) 在在高高度的环境的 maca。另外,一些复制 maca 基因在词法改编与功能被识别(即,叶卷应答) 并且不能生活的压力反应(即, GLYCINE 富有的 RNA 有约束力的蛋白质和 DNA-DAMAGE-REPAIR/TOLERATION 2 ) 在积极选择下面。一起, maca 染色体提供有用信息在安第斯山脉在植物的高高度的改编理解 WGD 的重要角色。 | Jing Zhang Yang Tian Liang Yan GuanghuiZhang Xiao Wang Yan Zeng Jiajin Zhang Xiao Ma Yuntao Tan Ni Long Yangzi Wang Yujin Ma Yuqi He Yu Xue Shumei Hao Shengchao Yang Wen Wang Liangsheng Zhang Yang Dong Wei Chen Jun Sheng | 2016 | Molecular Plant2016，9，7： | 9 |
| 4 | Basin assemblages and tectonic evolution in the Bohai Gulfand its neighbors显示文摘According to multi- disciplinary research advances in recent years, three blocks which named Jiaoliao - Bohai block, central North China block and Ordos block are divided. The differences of the tectonic association, types, styles and features in the space, and evolution from Archaean to Cenozoic in the time, between the former two blocks are discussed. Tectonic features and evolution in the four tectonic stages of Precambrian, Indo- China, Yanshanian and Cenozoic are emphasized especially here. All of them described above have inherit and neogenic property. The temporal-spatial change and interaction among basin assemblages in the above four stages are analyzed. Escape tectonic model is employed to interpret coeval compressive and extensional basins in the study area. | Li Sanzhong Xu Shumei Shan Yehua Li Xishuang Zhou Lihong Hao Yinquan Fan Zheqing Liang Huilan | 2000 | Acta Oceanologica Sinica2000，19，4： | 7 |
| 5 | A review of whole-process control of industrial volatile organic compounds in China显示文摘Volatile organic compounds(VOCs)play an important role in the formation of ground-level ozone and secondary organic aerosol(SOA),and they have been key issues in current air pollution prevention and control in China.Considerable attention has been paid to industrial activities due to their large and relatively complex VOCs emissions.The present research aims to provide a comprehensive review on whole-process control of industrial VOCs,which mainly includes source reduction,collection enhancement and end-pipe treatments.Lower VOCs materials including water-borne ones are the keys to source substitution in industries related to coating and solvent usage,leak detection and repair(LDAR)should be regarded as an efficient means of source reduction in refining,petrochemical and other chemical industries.Several types of VOCs collection methods such as gas-collecting hoods,airtight partitions and others are discussed,and airtight collection at negative pressure yields the best collection efficiency.Current end-pipe treatments like UV oxidation,low-temperature plasma,activated carbon adsorption,combustion,biodegradation,and adsorption-combustion are discussed in detail.Finally,several recommendations are made for future advanced treatment and policy development in industrial VOCs emission control. | Hailin Wang Shumei Sun Lei Nie Zhongshen Zhang Wenpeng Li Zhengping Hao | 2023 | Journal of Environmental Sciences2023，，1： | 4 |
| 6 | The effect of a conductive polyaniline/carbon black composite on the performance of a semiconductive layer显示文摘In this study,conductive polyaniline(PANI)ribbons were introduced to a semi-conductive layer to improve the conductivity stability of the layer during thermal expansion and enhance its ability to inhibit charge injection into the insulating layer.To maximise the effect of PANI,PANI and carbon black(CB)were preformed into a uniformly dispersed composite,which was then added to the polymer matrix of the semiconductive layer.The experimental results show that the resistivity of the semi-conductive layer containing the PANI/CB composite is more stable during thermal expansion than that of the semiconductive layer only doped with CB.This is attributed to the conductive CB network being enhanced by the PANI ribbons.In addition,due to the unique conductivity mechanism and high dielectric constant of PANI,the semiconductive layer has a strong ability to inhibit space charge injection into the insulating layer. | Enmin Wang Sheng Zhang Ciyao Wang Shumei Chen Chuncheng Hao Qingquan Lei | 2022 | High Voltage2022，7，2： | 0 |
| 7 | Effect of LiCoO_(2)on the positive temperature coefficient effect(PTC)properties of semiconductive composites and its ability to inhibit space charge injection显示文摘The semiconductive shield layer is between the conductive core and the insulation layer of high voltage direct current transmission cables and plays an important role in suppressing the accumulation of space charge in the insulation layer.Since the prevalent positive temperature coefficient effect(PTC)of the semiconducting layer leads to cable ageing and failure,this paper proposes the use of lithium cobaltate to modify the semiconducting shield to suppress its PTC effect and improve its ability to inhibit space charge injection.The ionic conductor LiCoO_(2)was prepared by the sol–gel method.LiCoO_(2)particles with particle sizes ranging from tens to hundreds of nanometres were obtained by ball milling.The prepared LiCoO_(2)was used as a filler to modify the carbon black(CB)/ethylene vinyl acetate copolymer(EVA)/low density polyethylene(LDPE)composites.After melt blending and moulding cross-linking,LiCoO_(2)/CB/EVA/LDPE semiconductive shielding specimens were successfully fabricated.Resistivity,Thermally Stimulated Depolarisation Currents(TSDC),and pulsed electroacoustic measurements were conducted for the prepared specimens.The PTC effect,which is common in polymer composites,was successfully suppressed,and the PTC strength was reduced by 17%.The resistivity of the semiconductive shielding layer was significantly reduced.The ability of the semiconducting shield to inhibit space charge injection from the conductive core to the insulation layer was significantly improved.When LiCoO_(2)content is 0.5 wt%,the semiconductive shielding layer has the best performance,and the space charge in the insulation layer was reduced by 70%compared to the undoped semiconducting shield. | Ciyao Wang Shumei Chen Yu Long Yujia Liu Chuncheng Hao Qingquan Lei | 2022 | High Voltage2022，7，5： | 0 |
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