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| 1 | Host metabolism dysregulation and cell tropism identification in human airway and alveolar organoids upon SARS-CoV-2 infection显示文摘The coronavirus disease 2019(COVID-19)pandemic is caused by infection with the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),which is spread primary via respiratory droplets and infects the lungs.Currently widely used cell lines and animals are unable to accurately mimic human physiological conditions because of the abnormal status of cell lines(transformed or cancer cells)and species differences between animals and humans.Organoids are stem cell-derived selforganized three-dimensional culture in vitro and model the physiological conditions of natural organs.Here we showed that SARS-CoV-2 infected and extensively replicated in human embryonic stem cells(hESCs)-derived lung organoids,including airway and alveolar organoids which covered the complete infection and spread route for SARS-CoV-2 within lungs.The infected ceils were ciliated,club,and alveolar type 2(AT2)cells,which were sequentially located from the proximal to the distal airway and terminal alveoli,respectively.Additionally,RNA-seq revealed early cell response to virus infection including an unexpected downregulation of the metabolic processes,especially lipid metabolism,in addition to the well-known upregulation of immune response.Further,Remdesivir and a human neutralizing antibody potently inhibited SARS-CoV-2 replication in lung organoids.Therefore,human lung organoids can serve as a pathophysiological model to investigate the underlying mechanism of SARS-CoV-2 infection and to discover and test therapeutic drugs for COVID-19. | Rongjuan Pei Jianqi Feng Yecheng Zhang Hao Sun Lian Li Xuejie Yang Jiangping He Shuqi Xiao Jin Xiong Ying Lin Kun Wen Hongwei Zhou Jiekai Chen Zhili Rong Xinwen Chen | 2021 | Protein & Cell2021，12，9： | 7 |
| 2 | In vitro effect of iASPP on cell growth of oral tongue squamous cell carcinoma显示文摘iASPP is an inhibitory member of the apoptosis-stimulating proteins of P53(ASPP) family. iASPP is over expressed in several malignant tumors and potentially affects cancer progression. However, the expression and potential role of iASPP in oral tongue squamous cell carcinoma(OTSCC) have not been addressed. In our study, we detected iASPP expression in OTSCC by immunohistochemistry. iASPP expression is up-regulated in OTSCC tissues. Moreover, in clinical pathology specimens, we found that increased iASPP expression correlates with poor differentiation and lymph node metastasis. Using multicellular tumor spheroids(MTS) and flow cytometry, we demonstrated that iASPP down-regulation arrests OTSCC cells at the G0/G1 phase, induces OTSCC cell apoptosis and inhibits OTSCC cell proliferation. These results indicate that iASPP plays a significant role in the progression of OTSCC and may serve as a biomarker or therapeutic target for OTSCC patients. | Yu Chen Wangxiang Yan Shuqi He Jiechun Chen Dan Chen Zhaoqiang Zhang Zhiguo Liu Xueqiang Ding Anxun Wang | 2014 | Chinese Journal of Cancer Research2014，26，4： | 7 |
| 3 | Recent progress in terahertz modulation using photonic structures based on two-dimensional materials显示文摘Terahertz(THz)technology has attracted great attention in the past few decades for its unique applications in various fields,including spectroscopy,noninvasive detection,wireless communications,and imaging.In parallel to this,the practical,fast,and broadband modulation of THz waves is becoming indispensable.Two-dimensional(2D)materials exhibit unusual optical and electrical properties,which has prompted tremendous interest and significant advances in THz modulation.This review provides the recent progress in 2D materials-based THz modulators,outlining the operating principles,including all-optical,electro-optic,magneto-optic,and other exotic mechanisms.We focus on the recent advances in THz modulation by the designed photonic structures,such as heterostructure,metamaterial,capacitor,optical cavity,and waveguide integration.Lastly,we discussed the challenges and opportunities for 2D materials-based THz modulators and presented our prospects for the future development. | Ziming Wang Jie Qiao Shuqi Zhao Shilei Wang Chuan He Xutang Tao Shanpeng Wang | 2021 | InfoMat2021，3，10： | 4 |
| 4 | From waste of marine culture to natural patch in cardiac tissue engineering显示文摘Sea squirt,as a highly invasive species and main biofouling source in marine aquaculture,has seriously threatened the biodiversity and aquaculture economy.On the other hand,a conductive biomaterial with excellent biocompatibility,and appropriate mechanical property from renewable resources is urgently required for tissue engineering patches.To meet these targets,we presented a novel and robust strategy for sustainable development aiming at the marine pollution via recycling and upgrading the waste biomass-sea squirts and serving as a renewable resource for functional bio-scaffold patch in tissue engineering.We firstly demonstrated that the tunic cellulose derived natural self-conductive scaffolds successfully served as functional cardiac patches,which significantly promote the maturation and spontaneous contraction of cardiomyocytes both in vitro and enhance cardiac function of MI rats in vivo.We believe this novel,feasible and“Trash to Treasure”strategy to gain cardiac patches via recycling the waste biomass must be promising and beneficial for marine environmental bio-pollution issue and sustainable development considering the large-scale consumption potential for tissue engineering and other applications. | Yutong He Honghao Hou Shuqi Wang Rurong Lin Leyu Wang Lei Yu Xiaozhong Qiu | 2021 | Bioactive Materials2021，6，7： | 2 |
| 5 | Genomic and transcriptomic analysis unveils population evolution and development of pesticide resistance in fall armyworm Spodoptera frugiperda显示文摘The fall armyworm(FAW),Spodoptera frugiperda,is a destructive pest native to America and has recently become an invasive insect pest in China.Because of its rapid spread and great risks in China,understanding of FAW genetic background and pesticide resistance is urgent and essential to develop effective management strategies.Here,we assembled a chromosome-level genome of a male FAW(SFynMstLFR)and compared re-sequencing results of the populations from America,Africa,and China.Strain identification of 163 individuals collected from America,Africa and China showed that both C and R strains were found in the American pop-ulations,while only C strain was found in the Chinese and African populations.Moreover,population geno-mics analysis showed that populations from Africa and China have close relationship with significantly genetic differentiation from American populations.Taken toge-ther,FAWs invaded into China were most likely origi-nated from Africa.Comparative genomics analysis displayed that the cytochrome p450 gene family is extremely expanded to 425 members in FAW,of which 283 genes are specific to FAW.Treatments of Chinese populations with twenty-three pesticides showed the variant patterns of transcriptome profiles,and several detoxification genes such as AOX,UGT and GST spe-cially responded to the pesticides.These findings will be useful in developing effective strategies for manage-ment of FAW in China and other invaded areas. | Furong Gui Tianming Lan Yue Zhao Wei Guo Yang Dong Dongming Fang Huan Liu Haimeng Li Hongli Wang Ruoshi Hao Xiaofang Cheng Yahong Li Pengcheng Yang Sunil Kumar Sahu Yaping Chen Le Cheng Shuqi He Ping Liu Guangyi Fan Haorong Lu Guohai Hu Wei Dong Bin Chen Yuan Jiang Yongwei Zhang Hanhong Xu Fei Lin Bernard Slippers Alisa Postma Matthew Jackson Birhan Addisie Abate Kassahun Tesfaye Aschalew Lemma Demie Meseret Destaw Bayeleygne Dawit Tesfaye Degefu Feng Chen Paul K.Kuria Zachary M.Kinyua Tong-Xian Liu Huanming Yang Fangneng Huang Xin Liu Jun Sheng Le Kang | 2022 | Protein & Cell2022，13，7： | 2 |
| 6 | Environmental economic geography:Recent advances and innovative development显示文摘Modern environmental problems are the result of human-environment interaction in the process of industrial-ization.The relationship between economic development and environmental quality is an important aspect of understanding human-environment interaction and has been widely concerned by multidisciplinary researchers.Economic geography(EG)is a discipline that mainly focuses on the location and connections of economic activi-ties.EG has developed to environmental economic geography(EEG)by incorporating environmental factors into its research framework,considering the operational mechanism of the co-evolution of economy and environment,and the dynamic process of green transition of techno-economic paradigms.Studying the resource and environ-mental effects of R&D,production,trade,and other aspects of the economic cycle is beneficial to realizing the“greening”economy.This paper traces the background and theoretical development path of EEG and elaborates on the interaction between EEG and other research paradigms on environmental problems.In recent years,the focuses of EEG are as follows:(1)the“location-environment”relationship of production from the perspective of local actors;(2)the“GVC/GPN-environment”relationship of multinational enterprises and space of flows from the perspective of the global network;(3)the“trade/circulation-environment”relationship from the perspective of national linkages;and(4)the co-evolution of the economic system and environment from the perspective of synergistic evolution,with a focus on innovation and institutional forces.The future development trend of EEG is combined with the endogenous growth theory and the sustainable development theory,mainly from the aspects of endogenous,system integration and multi-scale,to explain the differences in environmental issues of differ-ent regional economic activities,and to promote the development of EEG theory and interdisciplinary research,strengthening the construction of global and regional sustainable development system. | Canfei He Shuqi He Enyi Mu Jian Peng | 2022 | Geography and Sustainability2022，3，2： | 1 |
| 7 | Configuration and capacitance properties of polypyrrole/aligned carbon nanotubes synthesized by electropolymerization显示文摘Aligned carbon nanombes(ACNTs) were modified with polypyrrole(PPy) via electropolymerization.Because of the large specific surface area and excellent electrical conductivity of ACNTs,continuous electropolymerization was able to be carried out,forming a thick PPy coating on the ACNTs.The resulting nanocomposite possessed a core-shell structure with ACNTs as the core PPy as the shell,and nanoparticles of PPy on the top,and displayed high performance supercapacitance properties. | XU Ying ZHUANG ShuQi ZHANG XiaoYan HE PinGang FANG YuZhi | 2011 | Chinese Science Bulletin2011，56，35： | 1 |
| 8 | Determination of nitrite with the electrocatalytic property to the oxidation of nitrite on thionine modified aligned carbon nanotubes显示文摘 | Kun Zhao Haiyan Song Shuqi Zhuang Liming Dai Pingang He Yuzhi Fang | 2006 | Electrochemistry Communications2006，，1： | 1 |
| 9 | Insulin-like growth factor-2 enhances regulatory T cell functions and suppresses food allergy in an experimental model显示文摘 | Gui Yang Xiao-Rui Geng Jiang-Ping Song Yingying Wu Hao Yan Zhengke Zhan Litao Yang Weiyi He Zhi-Qiang Liu Shuqi Qiu Zhigang Liu Ping-Chang Yang | 2014 | The Journal of Allergy and Clinical Immunology2014，，： | 1 |
| 10 | Comparison of the longissimus muscle proteome between obese and lean pigs at 180 days显示文摘 | Anning Li Delin Mo Xiao Zhao Wei Jiang Peiqing Cong Zuyong He Shuqi Xiao Xiaohong Liu Yaosheng Chen | 2013 | Mammalian Genome2013，，1： | 1 |
| 11 | Structural basis of nucleosome deacetylation and DNA linker tightening by Rpd3S histone deacetylase complex显示文摘In Saccharomyces cerevisiae,cryptic transcription at the coding region is prevented by the activity of Sin3 histone deacetylase(HDAC)complex Rpd3S,which is carried by the transcribing RNA polymerase Ⅱ(RNAPⅡ)to deacetylate and stabilize chromatin.Despite its fundamental importance,the mechanisms by which Rpd3S deacetylates nucleosomes and regulates chromatin dynamics remain elusive.Here,we determined several cryo-EM structures of Rpd3S in complex with nucleosome core particles(NCPs),including the H3/H4 deacetylation states,the alternative deacetylation state,the linker tightening state,and a state in which Rpd3S co-exists with the Hho1 linker histone on NCP.These structures suggest that Rpd3S utilizes a conserved Sin3 basic surface to navigate through the nucleosomal DNA,guided by its interactions with H3K36 methylation and the extra-nucleosomal DNA linkers,to target acetylated H3K9 and sample other histone tails.Furthermore,our structures illustrate that Rpd3S reconfigures the DNA linkers and acts in concert with Hho1 to engage the NCP,potentially unraveling how Rpd3S and Hho1 work in tandem for gene silencing. | Shuqi Dong Huadong Li Meilin Wang Nadia Rasheed Binqian Zou Xijie Gao Jiali Guan Weijie Li Jiale Zhang Chi Wang Ningkun Zhou Xue Shi Mei Li Min Zhou Junfeng Huang He Li Ying Zhang Koon Ho Wong Xiaofei Zhang William Chong Hang Chao Jun He | 2023 | Cell Research2023，33，10： | 1 |
| 12 | A theoretical and deep learning hybrid model for predicting surface roughness of diamond-turned polycrystalline materials显示文摘Polycrystalline materials are extensively employed in industry.Its surface roughness significantly affects the working performance.Material defects,particularly grain boundaries,have a great impact on the achieved surface roughness of polycrystalline materials.However,it is difficult to establish a purely theoretical model for surface roughness with consideration of the grain boundary effect using conventional analytical methods.In this work,a theoretical and deep learning hybrid model for predicting the surface roughness of diamond-turned polycrystalline materials is proposed.The kinematic–dynamic roughness component in relation to the tool profile duplication effect,work material plastic side flow,relative vibration between the diamond tool and workpiece,etc,is theoretically calculated.The material-defect roughness component is modeled with a cascade forward neural network.In the neural network,the ratio of maximum undeformed chip thickness to cutting edge radius RT S,work material properties(misorientation angle θ_(g) and grain size d_(g)),and spindle rotation speed n s are configured as input variables.The material-defect roughness component is set as the output variable.To validate the developed model,polycrystalline copper with a gradient distribution of grains prepared by friction stir processing is machined with various processing parameters and different diamond tools.Compared with the previously developed model,obvious improvement in the prediction accuracy is observed with this hybrid prediction model.Based on this model,the influences of different factors on the surface roughness of polycrystalline materials are discussed.The influencing mechanism of the misorientation angle and grain size is quantitatively analyzed.Two fracture modes,including transcrystalline and intercrystalline fractures at different RTS values,are observed.Meanwhile,optimal processing parameters are obtained with a simulated annealing algorithm.Cutting experiments are performed with the optimal parameters,and a flat surface finish with Sa 1.314 nm is finally achieved.The developed model and corresponding new findings in this work are beneficial for accurately predicting the surface roughness of polycrystalline materials and understanding the impacting mechanism of material defects in diamond turning. | Chunlei He Jiwang Yan Shuqi Wang Shuo Zhang Guang Chen Chengzu Ren | 2023 | International Journal of Extreme Manufacturing2023，5，3： | 0 |
| 13 | 组蛋白分子伴侣FACT促进DNA复制偶联的核小体组装显示文摘真核生物DNA复制时,复制叉前的核小体解聚以允许复制体前行,同时在新合成的DNA子链上新的核小体必须马上组装,这被称为DNA复制偶联的核小体组装,是染色质复制的关键一步。 | Jiayi Yang Xu Zhang Jianxun Feng He Leng Shuqi Li Junyu Xiao Shaofeng Liu Zhiyun Xu Jiawei Xu Di Li Zhongshi Wang Jingyang Wang 李晴 | 2017 | 科学新闻2017，19，4： | 0 |
| 14 | SARS-CoV-2 spike host cell surface exposure promoted by a COPI sorting inhibitor显示文摘Via an insufficient coat protein complex I(COPI)retrieval signal,the majority of SARSCo V-2 spike(S)is resident in host early secretory organelles and a tiny amount is leaked out in cell surface.Only surface-exposed S can be recognized by B cell receptor(BCR)or anti-S therapeutic monoclonal antibodies(m Abs)that is the trigger step for B cell activation after S m RNA vaccination or infected cell clearance by S m Abs.Now,a drug strategy to promote S host surface exposure is absent.Here,we first combined structural and biochemical analysis to characterize S COPI sorting signals.A potent S COPI sorting inhibitor was then invented,evidently capable of promoting S surface exposure and facilitating infected cell clearance by S antibody-dependent cellular cytotoxicity(ADCC).Importantly,with the inhibitor as a probe,we revealed Omicron BA.1 S is less cell surface exposed than prototypes because of a constellation of S folding mutations,possibly corresponding to its ER chaperone association.Our findings not only suggest COPI is a druggable target against COVID-19,but also highlight SARS-Co V-2 evolution mechanism driven by S folding and trafficking mutations. | Yiqun Li Mingrui Yang Yanan Nan Jiaming Wang Sanjiao Wang Dongxiao Cui Jiajian Guo Pengfei He Wenxin Dai Shuqi Zhou Yue Zhang Wenfu Ma | 2023 | Acta Pharmaceutica Sinica B2023，13，7： | 0 |
| 15 | Genomic similarity and antibody-dependent enhancement of immune serum potentially affect the protective efficacy of commercial MLV vaccines against NADC30-like PRRSV显示文摘Porcine reproductive and respiratory syndrome(PRRS)is one of the most significant diseases affecting the pig industry worldwide.The PRRSV mutation rate is the highest among the RNA viruses.To date,NADC30-like PRRSV and highly pathogenic PRRSV(HP-PRRSV)are the dominant epidemic strains in China;however,commercial vaccines do not always provide sufficient cross-protection,and the reasons for insufficient protection are unclear.This study isolated a wild-type NADC30-like PRRSV,SX-YL1806,from Shaanxi Province.Vaccination challenge experiments in piglets showed that commercial modified live virus(MLV)vaccines provided good protection against HP-PRRSV.However,it could not provide sufficient protection against the novel strain SXYL1806.To explore the reasons for this phenomenon,we compared the genomic homology between the MLV strain and HP-PRRSV or NADC30-like PRRSV and found that the MLV strain had a lower genome similarity with NADC30-like PRRSV.Serum neutralization assay showed that MLV-immune serum slightly promoted the homologous HP-PRRSV replication and significantly promoted the heterologous NADC30-like PRRSV strain replication in vitro,suggesting that antibody-dependent enhancement(ADE)might also play a role in decreasing MLV protective efficacy.These findings expand our understanding of the potential factors affecting the protective effect of PRRSV MLV vaccines against the NADC30-like strains. | Yang Li Lele Xu Dian Jiao Zifang Zheng Zhihao Chen Yang Jing Zhiwei Li Zhiqian Maa Yingtong Feng Xuyang Guo Yumiao Wang Yuan He Haixue Zheng Shuqi Xiao | 2023 | Virologica Sinica2023，38，5： | 0 |
| 16 | MEinVR:Multimodal interaction techniques in immersive exploration显示文摘Immersive environments have become increasingly popular for visualizing and exploring large-scale,complex scientific data because of their key features:immersion,engagement,and awareness.Virtual reality offers numerous new interaction possibilities,including tactile and tangible interactions,gestures,and voice commands.However,it is crucial to determine the most effective combination of these techniques for a more natural interaction experience.In this paper,we present MEinVR,a novel multimodal interaction technique for exploring 3D molecular data in virtual reality.MEinVR combines VR controller and voice input to provide a more intuitive way for users to manipulate data in immersive environments.By using the VR controller to select locations and regions of interest and voice commands to perform tasks,users can efficiently perform complex data exploration tasks.Our findings provide suggestions for the design of multimodal interaction techniques in 3D data exploration in virtual reality. | Ziyue Yuan Shuqi He Yu Liu Lingyun Yu | 2023 | Visual Informatics2023，7，3： | 0 |
| 17 | Multifunctional biomimetic tactile system via a stick-slip sensing strategy for human–machine interactions显示文摘A tactile sensor system enables natural interaction between humans and machines;this interaction is crucial for dexterous robotic hands,interactive entertainment,and other smart scenarios.However,the lack of sliding friction detection significantly limits the accuracy and scope of interactions due to the absence of sophisticated information,such as slippage,material and roughness of held objects.Here,inspired by the stick-slip phenomena in the sliding process,we have developed a multifunctional biomimetic tactile system based on the stick-slip sensing strategy,which is a universal method to detect slippage and estimate the surface properties of objects by sliding.This system consists of a flexible fingertip-inspired tactile sensor,a read-out circuit and a machinelearning module.Based on the stick-slip sensing strategy,our system was endowed with high recognition rates for slippage detection(100.0%),material classification(93.3%)and roughness discrimination(92.8%).Moreover,robotic hand manipulation,interactive games and object classification are demonstrated with this multifunctional system for comprehensive and promising human-machine interactions. | Yue Li Manjun Zhao Yadong Yan Luanxi He Yingyi Wang Zuoping Xiong Shuqi Wang Yuanyuan Bai Fuqin Sun Qifeng Lu Yu Wang Tie Li Ting Zhang | 2022 | npj Flexible Electronics2022，6，1： | 0 |
| 18 | Study on partial discharge and charge accumulation characteristics of oil‐pressboard insulation under DC superimposed harmonic voltage显示文摘The oil‐pressboard insulation on the valve side of the converter transformer withstands pulsating square wave voltage.The voltage contains lots of DC components and high‐order harmonics,which is likely to cause insulation failures.The partial discharge and space charge experiments of oil‐impregnated pressboard under DC superimposed har-monic voltage are conducted to study the effects of harmonic frequency and DC com-ponents on the discharge and charge accumulation and to explore the correlation mechanism of charge dynamic behaviour and discharge.Firstly,it is found that high‐order harmonic voltage promotes the partial discharge generation,but DC component sup-presses the discharge.Moreover,the charge inside the pressboard is mainly injected in the same polarity and accumulates over time.The space charge density near the electrode increases with the increase of harmonic frequency,and the large amount of charge in-jection leads to electric field distortion.Meanwhile,the electric field direction changes faster,and the frequent charge injection and extraction behaviours make the charge recombination intensify,which promotes the discharge.Through the above analysis,the influence law of harmonic voltage on the failure of oil‐pressboard insulation is obtained,providing a theoretical basis for the structural optimisation and condition assessment of the converter transformer insulation. | Shuqi Li Qiang Wang Dongxin He Liangkai Wang Qingquan Li Wen Si | 2023 | High Voltage2023，8，6： | 0 |
| 19 | Structural mechanism of a dual-functional enzyme Dgp A/B/C as both a C-glycoside cleaving enzyme and an O-to C-glycoside isomerase显示文摘The C-glycosidic bond that connects the sugar moiety with aglycone is difficult to be broken or made due to its inert nature.The knowledge of C-glycoside breakdown and synthesis is very limited.Recently,the enzyme Dgp A/B/C cascade from a human intestinal bacterium PUE was identified to specifically cleave the C-glycosidic bond of puerarin(daidzein-8-C-glucoside).Here we investigated how puerarin is recognized and oxidized by Dgp A based on crystal structures of Dgp A with or without substrate and biochemical characterization.More strikingly,we found that apart from being a C-glycoside cleaving enzyme,Dgp A/B/C is capable of efficiently converting O-to C-glycoside showing the activity as a structure isomerase.A possible mechanistic model was proposed dependently of the simulated complex structure of Dgp B/C with 3’’-oxo-daidzin and structure-based mutagenesis.Our findings not only shed light on understanding the enzyme-mediated C-glycosidic bond breakage and formation,but also may help to facilitate stereospecific C-glycoside synthesis in pharmaceutical industry. | Pengfei He Sha Wang Sen Li Siqi Liu Shuqi Zhou Jing Wang Jiayue Tao Dongdong Wang Rufeng Wang Wenfu Ma | 2023 | Acta Pharmaceutica Sinica B2023，13，1： | 0 |
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