|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Host metabolism dysregulation and cell tropism identification in human airway and alveolar organoids upon SARS-CoV-2 infection显示文摘The coronavirus disease 2019(COVID-19)pandemic is caused by infection with the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),which is spread primary via respiratory droplets and infects the lungs.Currently widely used cell lines and animals are unable to accurately mimic human physiological conditions because of the abnormal status of cell lines(transformed or cancer cells)and species differences between animals and humans.Organoids are stem cell-derived selforganized three-dimensional culture in vitro and model the physiological conditions of natural organs.Here we showed that SARS-CoV-2 infected and extensively replicated in human embryonic stem cells(hESCs)-derived lung organoids,including airway and alveolar organoids which covered the complete infection and spread route for SARS-CoV-2 within lungs.The infected ceils were ciliated,club,and alveolar type 2(AT2)cells,which were sequentially located from the proximal to the distal airway and terminal alveoli,respectively.Additionally,RNA-seq revealed early cell response to virus infection including an unexpected downregulation of the metabolic processes,especially lipid metabolism,in addition to the well-known upregulation of immune response.Further,Remdesivir and a human neutralizing antibody potently inhibited SARS-CoV-2 replication in lung organoids.Therefore,human lung organoids can serve as a pathophysiological model to investigate the underlying mechanism of SARS-CoV-2 infection and to discover and test therapeutic drugs for COVID-19. | Rongjuan Pei Jianqi Feng Yecheng Zhang Hao Sun Lian Li Xuejie Yang Jiangping He Shuqi Xiao Jin Xiong Ying Lin Kun Wen Hongwei Zhou Jiekai Chen Zhili Rong Xinwen Chen | 2021 | Protein & Cell2021，12，9： | 7 |
| 2 | Productive HBV infection of well-differentiated, hNTCP-expressing human hepatoma-derived(Huh7) cells显示文摘Feasible and effective cell models for hepatitis B virus(HBV) infection are required for investigating the complete lifecycle of this virus, including the early steps of viral entry. Resistance to dimethyl sulfoxide/polyethylene glycol(DMSO/PEG), h NTCP expression, and a differentiated state are the limiting factors for successful HBV infection models. In the present study, we used a hepatoma cell line(Hu7^(hDNTCPh)) to overcome these limiting factors so that it exhibits excellent susceptibility to HBV infection. To achieve this goal, different hepatoma cell lines were tested with 2.5% DMSO/4%PEG8000, and one resistant cell line(Huh7 D) was used to construct a stable h NTCP-expressing cell line(Hu7^(hDNTCPh)) using a recombinant lentivirus system. Then, the morphological characteristics and differentiation molecular markers of Hu7^(hDNTCPh) cells with or without DMSO treatment were characterized. Finally, the susceptibility of Hu7^(hDNTCPh) cells to HBV infection was assessed. Our results showed that Huh7 D cells were resistant to 2.5% DMSO/4% PEG8000, whereas the others were not. Hu7^(hDNTCPh) cells were established to express a high level of h NTCP compared to liver extracts, and Hu7^(hDNTCPh) cells rapidly transformed into a non-dividing, well-differentiated polarized phenotype under DMSO treatment. Hu7^(hDNTCPh) cells fully supported the entire lifecycle of HBV infection. This cell culture system will be useful for the analysis of host-virus interactions, which should facilitate the discovery of antiviral drugs and vaccines. | Ming Zhou Kaitao Zhao Yongxuan Yao Yifei Yuan Rongjuan Pei Yun Wang Jizheng Chen Xue Hu Yuan Zhou Xinwen Chen Chunchen Wu | 2017 | Virologica Sinica2017，32，6： | 7 |
| 3 | Persistent hepatitis C virus infections and hepatopathological manifestations in immune-competent humanized mice显示文摘 | Jizheng Chen Yang Zhao Chao Zhang Hairong Chen Jin Feng Xiumei Chi Yu Pan Jun Du Min Guo Huang Cao Honghe Chen Zilong Wang Rongjuan Pei Qian Wang Lei Pan Junqi Niu Xinwen Chen Hong Tang | 2014 | Cell Research2014，24，9： | 5 |
| 4 | Phosphatidylserine-Specific Phospholipase A1 is the Critical Bridge for Hepatitis C Virus Assembly显示文摘The phosphatidylserine-specific phospholipase A1(PLA1A)is an essential host factor in hepatitis C virus(HCV)assembly.In this study,we mapped the E2,NS2 and NS5A involved in PLA1A interaction to their lumenal domains and membranous parts,through which they form oligomeric protein complexes to participate in HCV assembly.Multiple regions of PLA1A were involved in their interaction and complex formation.Furthermore,the results represented structures with PLA1A and E2 in closer proximity than NS2 and NS5A,and strongly suggest PLA1 A-E2,s physical interaction in cells.Meanwhile,we mapped the NS5A sequence which participated in PLA1A interaction with the C-terminus of domain 1.Interestingly,these amino acids in the sequence are also essential for viral RNA replication.Further experiments revealed that these four proteins interact with each other.Moreover,PLA1A expression levels were elevated in livers from HCV-infected patients.In conclusion,we exposed the structural determinants of PLA1A,E2,NS2 and NS5A proteins which were important for HCV assembly and provided a detailed characterization of PLA1A in HCV assembly. | Qi Yang Min Guo Yuan Zhou Xue Hu Yun Wang Chunchen Wu Min Yang Rongjuan Pei Xinwen Chen Jizheng Chen | 2019 | Virologica Sinica2019，34，5： | 4 |
| 5 | Nasal delivery of broadly neutralizing antibodies protects mice from lethal challenge with SARS-CoV-2 delta and omicron variants显示文摘Multiple new variants of severe acute respiratory syndrome coronavirus 2(SARS-Co V-2)have constantly emerged,as the delta and omicron variants,which have developed resistance to currently gained neutralizing antibodies.This highlights a critical need to discover new therapeutic agents to overcome the variants mutations.Despite the availability of vaccines against coronavirus disease 2019(COVID-19),the use of broadly neutralizing antibodies has been considered as an alternative way for the prevention or treatment of SARS-Co V-2 variants infection.Here,we show that the nasal delivery of two previously characterized broadly neutralizing antibodies(F61 and H121)protected K18-h ACE2 mice against lethal challenge with SARS-Co V-2 variants.The broadly protective efficacy of the F61 or F61/F121 cocktail antibodies was evaluated by lethal challenge with the wild strain(WIV04)and multiple variants,including beta(B.1.351),delta(B.1.617.2),and omicron(B.1.1.529)at 200or 1000 TCID_(50),and the minimum antibody administration doses(5-1.25 mg/kg body weight)were also evaluated with delta and omicron challenge.Fully prophylactic protections were found in all challenged groups with both F61 and F61/H121 combination at the administration dose of 20 mg/kg body weight,and corresponding mice lung viral RNA showed negative,with almost all alveolar septa and cavities remaining normal.Furthermore,low-dose antibody treatment induced significant prophylactic protection against lethal challenge with delta and omicron variants,whereas the F61/H121 combination showed excellent results against omicron infection.Our findings indicated the potential use of broadly neutralizing monoclonal antibodies as prophylactic and therapeutic agent for protection of current emerged SARS-Co V-2 variants infection. | Jia Lu Qiangling Yin Rongjuan Pei Qiu Zhang Yuanyuan Qu Yongbing Pan Lina Sun Ding Gao Cuiqin Liang Jingwen Yang Wei Wu Jiandong Li Zongqiang Cui Zejun Wang Xinguo Li Dexin Li Shiwen Wang Kai Duan Wuxiang Guan Mifang Liang Xiaoming Yang | 2022 | Virologica Sinica2022，37，2： | 4 |
| 6 | Distribution of airborne SARS-CoV-2 and possible aerosol transmission in Wuhan hospitals, China显示文摘The novel severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has caused an outbreak of CO V ID-19(2019 coronavirus infectious disease)that has led to a global public health crisis.The spread of SARS-CoV-2 can be rapid;but there is still considerable controversy about its main route of transmission[l,2];that is,whether transmission is by contact or through the air[2,3].Recently the W H O(World Health Organization)released a document that recognized the possibility of aerosol transmission of SARS-CoV-2 for indoor environments and enclosed spaces[4].The purpose of the present study was to evaluate the airborne transmission of SARS-CoV-2 through a field investigation of its occurrence in the air and other samples collected from health facilities in W uhan;China. | Jia Hu Chengfeng Lei Zhen Chen Weihua Liu Xujuan Hu Rongjuan Pei Zhengyuan Su Fei Deng Yu Huang Xiulian Sun Junji Cao Wuxiang Guan | 2020 | National Science Review2020，7，12： | 4 |
| 7 | In-cell infection: a novel pathway for Epstein-Barr virus infection mediated by cell-in-cell structures显示文摘 | Chao Ni Yuhui Chen Musheng Zeng Rongjuan Pei Yong Du Linquan Tang Mengyi Wang Yazhuo Hu Hanyu Zhu Meifang He Xiawei Wei Shan Wang Xiangkai Ning Manna Wang Jufang Wang Li Ma Xinwen Chen Qiang Sun Hong Tang Ying Wang Xiaoning Wang | 2015 | Cell Research2015，25，7： | 3 |
| 8 | Host HDAC4 regulates the antiviral response by inhibiting the phosphorylation of IRF3显示文摘Class II HDACs, such as HDAC4, are critical regulators of the immune response in various immune cells;however, its role in innate immunity remains largely unknown.Here, we report that the overexpression of HDAC4 suppresses the production of type I interferons triggered by pattern-recognition receptors (PRRs). HDAC4 repressed the translocation of transcription factor IRF3 to the nucleus, thereby decreasing IRF3-mediated IFN-β expression. In particular, we also determined that HDAC4 can be phosphorylated and simultaneously block the phosphorylation of IRF3 at Ser386 and Ser396 by TBK1 and IKKε, respectively, by interacting with the kinase domain of TBK1 and IKKε. Furthermore, IFN-β may stimulate the expression of HDAC4. Our findings suggest that HDAC4 acts as a regulator of PRR signaling and is a novel mechanism of negative feedback regulation for preventing an overreactive innate immune response. | Qi Yang Jielin Tang Rongjuan Pei XiaoXiao Gao Jing Guo Chonghui Xu Yun Wang QianWang Chunchen Wu Yuan Zhou Xue Hu He Zhao Yanyi Wang Xinwen Chen Jizheng Chen | 2019 | Journal of Molecular Cell Biology2019，11，2： | 2 |
| 9 | Different Responses of Two Highly Permissive Cell Lines Upon HCV Infection显示文摘The construction of the first infectious clone JFH-1 speeds up the research on hepatitis C virus (HCV). However, Huh7 cell line was the only highly permissive cell line for HCV infection and only a few clones were fully permissive. In this study, two different fully permissive clones of Huh7 cells, Huh7.5.1 and Huh7-Lunet-CD81 (Lunet-CD81) cells were compared for their responses upon HCV infection. The virus replication level was found slightly higher in Huh7.5.1 cells than that in Lunet-CD81 cells. Viability of Huh7.5.1 cells but not of Lunet-CD81 cells was reduced significantly after HCV infection. Further analysis showed that the cell cycle of infected Huh7.5.1 cells was arrested at G1 phase. The G1/S transition was blocked by HCV infection in Huh7.5.1 cells as shown by the cell cycle synchronization analysis. Genes related to cell cycle regulation was modified by HCV infection and gene interaction analysis in GeneSpring GX in Direct Interactions mode highlighted 31 genes. In conclusion, the responses of those two cell lines were different upon HCV infection. HCV infection blocked G1/S transition and cell cycle progress, thus reduced the cell viability in Huh7.5.1 cells but not in Lunet-CD81 cells. Lunet-CD81 cells might be suitable for long term infection studies of HCV. | Honghe Chen Rongjuan Pei Xinwen Chen | 2013 | Virologica Sinica2013，28，4： | 2 |
| 10 | Regulation of Hepatitis C Virus Replication and Gene Expression by the MAPK-ERK Pathway显示文摘The mitogen activated protein kinases-extracellular signal regulated kinases (MAPK-ERK) pathway is involved in regulation of multiple cellular processes including the cell cycle. In the present study using a Huh7 cell line Con1 with an HCV replicon, we have shown that the MAPK-ERK pathway plays a significant role in the modulation of HCV replication and protein expression and might influence IFN-α signalling. Epithelial growth factor (EGF) was able to stimulate ERK activation and decreased HCV RNA load while a MAPK-ERK pathway inhibitor U0126 led to an elevated HCV RNA load and higher NS5A protein amounts in Con1 cells. It could be further demonstrated that the inhibition of the MAPK-ERK pathway facilitated the translation directed by the HCV internal ribosome entry site. Consistently, a U0126 treatment enhanced activity of the HCV reporter replicon in transient transfection assays. Thus, the MAPK-ERK pathway plays an important role in the regulation of HCV gene expression and replication. In addition, cyclin-dependent kinases (CDKs) downstream of ERK may also be involved in the modulation of HCV replication since roscovitine, an inhibitor of CDKs had a similar effect to that of U0126. Modulation of the cell cycle progression by cell cycle inhibitor or RNAi resulted consistently in changes of HCV RNA levels. Further, the replication of HCV replicon in Con1 cells was inhibited by IFN-α. The inhibitory effect of IFN-α could be partly reversed by pre-incubation of Con-1 cells with inhibitors of the MAPK-ERK pathway and CDKs. It could be shown that the MAPK-ERK inhibitors are able to partially modulate the expression of interferon-stimulated genes. | Rongjuan Pei Xiaoyong Zhang Song Xu Zhongji Meng Michael Roggendorf Mengji Lu Xinwen Chen | 2012 | Virologica Sinica2012，27，5： | 2 |
| 11 | Ozone Water Is an Effective Disinfectant for SARS-CoV-2显示文摘Dear Editor,The severe acute respiratory syndrome coronavirus 2(SARS-Co V-2)is the causative pathogen of the pandemic coronavirus disease 2019(COVID-19),which has infected nearly 90 million people and resulted in 2 million human deaths by the end of the year 2020.SARS-Co V-2 can survive in aerosols or on the surfaces of various materials for up to 72 h(Ong et al.2020;van Doremalen et al.2020).In China,although the human-to-human transmission pathway is under well control,the fomite transmission pathway now accounts for a significant fraction of new infection cases.Therefore,environmental disinfection of public areas and workspaces potentially contaminated by SARS-Co V-2 is an important measure to control the spread of COVID-19 by fomite transmission.A disinfectant that is safe and readily available at a low cost is urgently needed to meet environmental disinfection requirements. | Xiao Hu Zhen Chen Zhengyuan Su Fei Deng Xinwen Chen Qi Yang Pan Li Quanjiao Chen Jun Ma Wuxiang Guan Rongjuan Pei Yun Wang | 2021 | Virologica Sinica2021，36，5： | 2 |
| 12 | Hepatitis C virus-induced prion protein expression facilitates hepatitis C virus replication显示文摘Hepatitis C virus(HCV) infects approximately 180 million people worldwide. Significant progress has been made since the establishment of in vitro HCV infection models in cells. However, the replication of HCV is complex and not completely understood. Here, we found that the expression of host prion protein(Pr P) was induced in an HCV replication cell model. We then showed that increased Pr P expression facilitated HCV genomic replication. Finally, we demonstrated that the KKRPK motif on the N-terminus of Pr P bound nucleic acids and facilitated HCV genomic replication. Our results provided important insights into how viruses may harness cellular protein to achieve propagation. | Huixia Zhang Shanshan Gao Rongjuan Pei Xinwen Chen Chaoyang Li | 2017 | Virologica Sinica2017，32，6： | 2 |
| 13 | Contribution of Temperature Increase to Restrain the Transmission of COVID-19显示文摘The COVID-19 outbreak has already become a global pandemic and containing this rapid worldwide transmission is of great challenge.The impacts of temperature and humidity on the COVID-19 transmission rate are still under discussion.Here,we elucidated these relationships by utilizing two unique scenarios,repeated measurement and natural experiment,using the COVID-19 cases reported from January 23–February 21,2020,in China.The modeling results revealed that higher temperature was most strongly associated with decreased COVID-19 transmission at a lag time of 8 days.Relative humidity(RH)appeared to have only a slight effect.These findings were verified by assessing SARSCoV-2 infectivity under the relevant conditions of temperature(4C–37C)and RH(>40%).We concluded that temperature increase made an important,but not determined,contribution to restrain the COVID19 outbreak in China.It suggests that the emphasis of other effective controlling polices should be strictly implemented to restrain COVID19 transmission in cold seasons. | Mengyuan Ren Rongjuan Pei Bahabaike Jiangtulu Junxi Chen Tao Xue Guofeng Shen Xiaoru Yuan Kexin Li Changxin Lan Zhen Chen Xinwen Chen Yun Wang Xiaoqian Jia Zewu Li Audil Rashid Tippawan Prapamontol Xiuge Zhao Zhaomin Dong Yali Zhang Le Zhang Rongwei Ye Zhiwen Li Wuxiang Guan Bin Wang | 2021 | The Innovation2021，2，1： | 2 |
| 14 | Polymerase mutations rtN238R,rtT240Y and rtN248H of hepatitis B virus decrease susceptibility to adefovir显示文摘Long term antiviral therapy with nucleos(t)ide analogs(NAs) may lead to the emergence of drug-resistance viral mutants in chronic hepatitis B virus(HBV) patient.The purpose of this study was to identify adefovir dipivoxil(ADV) resistance mutations of HBV polymerase and determine effective drugs to replace ADV.The reverse transcriptase(RT) coding region was PCR-amplified using HBV DNA extracted from patient blood samples and sequenced.Nineteen substitution mutations were detected.Among them,rtN238R,rtT240Y and rtN248H were often observed in patients receiving ADV administration.These three potential drug resistant sites were introduced into HBV replication-competent plasmids.The in vitro susceptibility of both wild-type(WT) and mutant-type(MT) HBV to NAs was analyzed by Southern blotting and quantitative real-time PCR.The rtN238R,rtT240Y and rtN248H substitutions had no obvious effect on HBV DNA replication or gene expression.The in vitro susceptibility analysis showed that rtN238R,rtT240Y and rtN248H substitutions were responsible for the reduced susceptibility to ADV,and demonstrated a 5.42-,2.89-and 5.72-fold increase in resistance towards ADV,respectively.However,HBV harbored these mutations retained normal susceptibility to LMV,LdT,ETV and TDF. | QIN Bo PEI RongJuan HE TingTing HUANG ZhaoHui PAN GuoShao TU ChunYu LU MengJi CHEN XinWen | 2013 | Chinese Science Bulletin2013，58，15： | 1 |
| 15 | SARS-CoV-2 Does Not Replicate in Aedes Mosquito Cells nor Present in Field-Caught Mosquitoes from Wuhan显示文摘Dear Editor,Severe acute respiratory syndrome coronavirus 2(SARSCoV-2), the etiologic agent of COVID-19, is an enveloped,positive-sense single-stranded RNA virus that first discovered in December 2019 from a seafood market in Wuhan, China(Zhou et al. 2020). | Han Xia Evans Atoni Lu Zhao Nanjie Ren Doudou Huang Rongjuan Pei Zhen Chen Jin Xiong Raphael Nyaruaba Shuqi Xiao Bo Zhang Zhiming Yuan | 2020 | Virologica Sinica2020，35，3： | 1 |
| 16 | Hepatitis B virus suppresses toll‐like receptor–mediated innate immune responses in murine parenchymal and nonparenchymal liver cells显示文摘 | Jun Wu Zhongji Meng Min Jiang Rongjuan Pei Martin Trippler Ruth Broering Agnes Bucchi Jan‐Peter Sowa Ulf Dittmer Dongliang Yang Michael Roggendorf Guido Gerken Mengji Lu Joerg F. Schlaak | 2009 | Hepatology2009，，： | 1 |
| 17 | Construction of a chimeric hepatitis C virus replicon based on a strain isolated from a chronic hepatitis C patient显示文摘Subgenomic repiicons of hepatitis C virus(HCV) have been widely used for studying HCV replication.Here,we report a new subgenomic replicon based on a strain isolated from a chronically infected patient.The coding sequence of HCV was recovered from a Chinese chronic hepatitis C patient displaying high serum HCV copy numbers.A consensus sequence designated as CCH strain was constructed based on the sequences of five clones and this was classified by sequence alignment as belonging to genotype 2a.The subgenomic replicon of CCH was replication-deficient in cell culture,due to dysfunctions in NS3 and NS5B.Various JFH1/CCH chimeric repiicons were constructed,and specific mutations were introduced.The introduction of mutations could partially restore the replication of chimeric repiicons.A replication-competent chimeric construct was finally obtained by the introduction of NS3 from JFH1 into the backbone of the CCH strain. | Huang Cao Wandi Zhu Qingxia Han Rongjuan Pei Xinwen Chen | 2014 | Virologica Sinica2014，29，1： | 1 |
| 18 | Efficient assembly of a large fragment of monkeypox virus genome as a qPCR template using dual-selection based transformation-associated recombination显示文摘Transformation-associated recombination(TAR)has been widely used to assemble large DNA constructs.One of the significant obstacles hindering assembly efficiency is the presence of error-prone DNA repair pathways in yeast,which results in vector backbone recircularization or illegitimate recombination products.To increase TAR assembly efficiency,we prepared a dual-selective TAR vector,pGFCS,by adding a PADH1-URA3 cassette to a previously described yeast-bacteria shuttle vector,p GF,harboring a PHIS3–HIS3 cassette as a positive selection marker.This new cassette works as a negative selection marker to ensure that yeast harboring a recircularized vector cannot propagate in the presence of 5-fluoroorotic acid.To prevent pGFCS bearing ura3 from recombining with endogenous ura3-52 in the yeast genome,a highly transformable Saccharomyces cerevisiae strain,VL6-48B,was prepared by chromosomal substitution of ura3-52 with a transgene conferring resistance to blasticidin.A55-kb genomic fragment of monkeypox virus encompassing primary detection targets for quantitative PCR was assembled by TAR using pGFCS in VL6-48B.The pGFCS-mediated TAR assembly showed a zero rate of vector recircularization and an average correct assembly yield of 79%indicating that the dual-selection strategy provides an efficient approach to optimizing TAR assembly. | Lei Yang Lingqian Tian Leshan Li Qiuhong Liu Xiang Guo Yuan Zhou Rongjuan Pei Xinwen Chen Yun Wang | 2022 | Virologica Sinica2022，37，3： | 1 |
| 19 | Enhanced host immune responses in presence of HCV facilitate HBV clearance in coinfection显示文摘Hepatitis B virus(HBV)/Hepatitis C virus(HCV)coinfection is frequently observed because of the common infection routine.Despite the reciprocal inhibition exerted by HBV and HCV genomes,the coinfection of HBV and HCV is associated with more severe forms of liver diseases.However,the complexity of viral interference and underlying pathological mechanism is still unclarified.With the demonstration of absence of direct viral interplay,some in vitro studies suggest the indirect effects of viral-host interaction on viral dominance outcome.Here,we comprehensively investigated the viral replication and host immune responses which might mediate the interference between viruses in HBV/HCV coinfected Huh7-NTCP cells and immunocompetent HCV human receptors transgenic ICR mice.We found that presence of HCV significantly inhibited HBV replication in vitro and in vivo irrespective of the coinfection order,while HBV did not affect HCV replication.Pathological alteration was coincidently reproduced in coinfected mice.In addition to the participation of innate immune response,an involvement of HCV in up-regulating HBV-specific immune responses was described to facilitate HBV clearance.Our systems partially recapitulate HBV/HCV coinfection and unveil the uncharacterized adaptive anti-viral immune responses during coinfection,which renews the knowledge on the nature of indirect viral interaction during HBV/HCV coinfection. | Shuhui Liu Kaitao Zhao Xi Su Xiaoxiao Gao Yongxuan Yao Ranran Kong Yun Wang Chunchen Wu Mengji Lu Xinwen Chen Rongjuan Pei | 2022 | Virologica Sinica2022，37，3： | 1 |
| 20 | RNA binding protein 24 regulates the translation and replication of hepatitis C virus显示文摘 | Huang Cao Kaitao Zhao Yongxuan Yao Jing Guo Xiaoxiao Gao Qi Yang Min Guo Mengji Lut Xinwen Chen Rongjuan Pei | 2018 | Protein & Cell2018，9，11： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员