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| 1 | Comparative Proteomic Analysis of Arabidopsis Mature Pollen and Germinated Pollen显示文摘Proteomic analysis was applied to generating the map of Arabidopsis mature pollen proteins and analyzing the differentially expressed proteins that are potentially involved in the regulation of Arabidopsis pollen germination.By applying 2-D electrophoresis and silver staining,we resolved 499 and 494 protein spots from protein samples extracted from pollen grains and pollen tubes,respectively.Using the matrix-assisted laser desorption ionization time-of-flight mass spectrometry method,we identified 189 distinct proteins from 213 protein spots expressed in mature pollen or pollen tubes,and 75 new identified proteins that had not been reported before in research into the Arabidopsis pollen proteome.Comparative analysis revealed that 40 protein spots exhibit reproducible significant changes between mature pollen and pollen tubes.And 21 proteins from 17 downregulated and six upregulated protein spots were identified.Functional category analysis indicated that these differentially expressed proteins mainly involved in signaling,cellular structure,transport,defense/stress responses,transcription,metabolism,and energy production.The patterns of changes at protein level suggested the important roles for energy metabolism-related proteins in pollen tube growth,accompanied by the activation of the stress response pathway and modifications to the cell wall. | Junjie Zou Lianfen Song Wenzheng Zhang Yi Wang Songlin Ruan Wei-Hua Wu | 2009 | Journal of Integrative Plant Biology2009，51，5： | 5 |
| 2 | A zinc finger protein, interacted with cyclophilin,affects root development via IAA pathway in rice显示文摘The plant hormone auxin plays a crucial role in lateral root development. To better understand the molecular mechanisms underlying lateral root formation,an auxin-responsive gene OsCYP2(Os02g0121300) was characterized from rice. Compared to the wild type,OsCYP2-RNAi(RNA interference) lines exhibited distinctive defects in lateral root development. Yeast two-hybrid and glutathione S-transferase pull-down results confirmed that OsCYP2 interacted with a C2HC-type zinc finger protein(OsZFP, Os01g0252900) which is located in the rice nucleus. T_2 OsZFP-RNAi lines had significantly fewer lateral roots than did wild-type plants, which suggests a role for OsCYP2 and OsZFP in regulating lateral root development.Quantitative real-time polymerase chain reaction showed that the expression of certain Aux/IAA(auxin/indole-3-acetic acid) genes was altered in OsCYP2-and Os ZFP-RNAi lines in response to IAA. These findings imply that OsCYP2 and OsZFP participate in IAA signal pathways controlling lateral root development. More importantly, OsIAA11 showed functional redundancy not only in OsCYP2-RNAi lines but also in Os ZFP-RNAi lines, which provides important clues for the elucidation of mechanisms controlling lateral root development in response to auxin. | Peng Cui Hongbo Liu Songlin Ruan Basharat Ali Rafaqat Ali Gill Huasheng Ma Zhifu Zheng Weijun Zhou | 2017 | Journal of Integrative Plant Biology2017，59，7： | 4 |
| 3 | Near‑Instantaneously Self‑Healing Coating toward Stable and Durable Electromagnetic Interference Shielding显示文摘Durable electromagnetic interference(EMI)shielding is highly desired,as electromagnetic pollution is a great concern for electronics’stable performance and human health.Although a superhydrophobic surface can extend the service lifespan of EMI shielding materials,degradation of its protection capability and insufficient self-healing are troublesome issues due to unavoidable physical/chemical damages under long-term application conditions.Here,we report,for the first time,an instantaneously self-healing approach via microwave heating to achieve durable shielding performance.First,a hydrophobic 1H,1H,2H,2H-perfluorooctyltriethoxysilane(POTS)layer was coated on a polypyrrole(PPy)-modified fabric(PPy@POTS),enabling protection against the invasion of water,salt solution,and corrosive acidic and basic solutions.Moreover,after being damaged,the POTS layer can,for the first time,be instantaneously self-healed via microwave heating for a very short time,i.e.,4 s,benefiting from the intense thermal energy generated by PPy under electromagnetic wave radiation.This self-healing ability is also repeatable even after intentionally severe plasma etching,which highlights the great potential to achieve robust and durable EMI shielding applications.Significantly,this approach can be extended to other EMI shielding materials where heat is a triggering stimulus for healing thin protection layers.We envision that this work could provide insights into fabricating EMI shielding materials with durable performance for portable and wearable devices,as well as for human health care. | Lihua Zou Chuntao Lan Songlin Zhang Xianhong Zheng Zhenzhen Xu Changlong Li Li Yang Fangtao Ruan Swee Ching Tan | 2021 | Nano-Micro Letters2021，13，11： | 3 |
| 4 | Genetic Transformation of Chinese Cabbage (Brassica rapa pekinensis) with Floral-dip Method显示文摘[Objective] This study is aimed to transform gus gene into Chinese cabbage (Brassica rapa pekinensis) by floral-dip method. [Method] The Chinese cabbage was transformed by floral-dip method for the first time. Then the cabbage seeds were harvested and screened by hygromycin, and the plants with resistance were confirmed by histochemical GUS assay and PCR detection. [Result] The target gene was successfully integrated into the Chinese cabbage genome, and the transformation rate was 0.1%. [Conclusion] This study optimized the genetic transformation system for Chinese cabbage, and laid the foundation for improving the means of genetic transformation of Chinese cabbage. | Yang GAO Xiangliang REN Songlin RUAN Huoxu WANG | 2012 | Agricultural Biotechnology2012，1，4： | 2 |
| 5 | Expression of microRNAs and its regulation in plants显示文摘 | Zhixin Xie Kanhav Khanna Songlin Ruan | 2010 | Seminars in Cell and Developmental Biology2010，，8： | 1 |
| 6 | A study on shrinkages during the carbonization of bamboo显示文摘 | Zuo Songlin GAO Shangyu RUAN Xigen | 2003 | Journal of Nanjing Forestry University (Natural Sciences Edition)2003，3，27： | 1 |
| 7 | Cadmium induced a non-coding RNA microRNA535 mediates Cd accumulation in rice显示文摘Identifying key regulators related to cadmium(Cd)tolerance and accumulation is the main factor for genetic engineering to improve plants for bioremediation and ensure crop food safety.MicroRNAs(miRNAs),as fine-tuning regulators of genes,participate in various abiotic stress processes.MiR535 is an ancient conserved non-coding small RNA in land plants,positively responding to Cd stress.We investigated the effects of knocking out(mir535)and overexpressing miR535(mir535 and OE535)under Cd stress in rice plants in this study.The mir535 plants showed better Cd tolerance than wild type(WT),whereas the OE535 showed the opposite effect.Cd accumulated approximately 71.9%and 127%in the roots of mir535 and OE535 plants,respectively,compared to WT,after exposure to 2μmol/L Cd.In brown rice,the total Cd accumulation of OE535 and mir535 was about 78%greater and 35%lower than WT.When growing in 2 mg/kg Cd of soil,the Cd concentration was significantly lower in mir535 and higher in OE535 than in the WT;afterward,we further revealed the most possible target gene SQUAMOSA promoter binding-like transcription factor 7(SPL7)and it negatively regulates Nramp5 expression,which in turn regulates Cd metabolism.Therefore,the CRISPR/Cas9 technology may be a valuable strategy for creating new rice varieties to ensure food safety. | Erkui Yue Fuxi Rong Zhen Liu Songlin Ruan Tao Lu Haifeng Qian | 2023 | Journal of Environmental Sciences2023，，8： | 1 |
| 8 | The influence of priming on germination of rice seeds and seedling emergence and perfomance in flooded soil显示文摘 | Ruan Songlin Xue Qingzhong Tylkowska K | 2002 | Seed Science and Technology2002，30，： | 1 |
| 9 | Quantitative detection of Cymbidium mosaic virus by real time PCR显示文摘The technique of SYBR Green-based quantitative real-time reverse transcription polymerase chain reaction(real-time RT-PCR)was applied to quantitative detect a 764 bp nucleotide sequence containing total coat protein(cp)gene of Cymbidium mosaic virus(CyMV).The plasmid containing the target sequence was constructed to prepare the standard curve and detect the sensitivity.The standard curve was drawn based on the linear relationship between the logarithm(base 10)of the quantity of target sequence and cycle threshold[C(T)].While the concentration of plasmid DNA falling within the range of 2.6×10^(7)to 2.6×10^(2)copies per tube established a regression equation,y=-0.3583x+10.32,and related coefficient:r^(2)=0.995.The real-time RT-PCR assay for CyMV had a minimum detectable quantity of two copies per tube.The naturally infected samples of Phalaenopsis sp.and the artificially inoculated samples of Arachnis sp.with trace CyMV were quantitatively detected using this method.CyMVin the positive samples of Phalaenopsis sp.and Arachnis sp.was confirmed by DNA sequencing and cp gene homeology blast.The results showed that CyMV extracted from the leaves of orchid in Hangzhou,Zhejiang Province,China,could be derived from Kunming city(KM),Yunnan Province,China.This method characterized by high sensitivity,specificity,and precision is suitable for early diagnosis and quantitative detection of CyMV. | Aichun LIU Yun ZHAO Songlin RUAN Guozheng SHEN | 2009 | Frontiers in Biology2009，4，3： | 1 |
| 10 | The influence of priming on germination of rice seeds and seedling emergence and perfomance in flooded soil显示文摘 | Ruan Songlin Xue Qingzhong Tylkowska K | | 0，，： | 1 |
| 11 | Advances in plant proteomics—key techniques of proteome显示文摘Following the completion of genome sequencing of model plants,such as rice(Oryza sativa L.)and Arabidopsis thaliana,the era of functional plant genomics has arrived which provides a solid basis for the develop-ment of plant proteomics.We review the background and concepts of proteomics,as well as the key techniques which include:(1)separation techniques such as 2-DE(two-dimensional electrophoresis),RP-HPLC(reverse phase high performance liquid chromatography)and SELDI(surface enhanced laser desorption/ionization)protein chip;(2)mass spectrometry such as MALDI-TOF-MS(matrix assisted laser desorption/ionization-time of flight-mass spectrometry)and ESI-MS/MS(electrospray ionization mass spectrometry/mass spectrometry);(3)Peptide sequence tags;(4)databases related to proteomics;(5)quantitative proteome;(6)TAP(tandem affinity purification)and(7)yeast two-hybrid system.In addition,the challenges and prospects of pro-teomics are also discussed. | Songlin RUAN Huasheng MA Shiheng WANG Ya XIN Lihua QIAN Jianxing TONG Jie WANG | 2008 | Frontiers in Biology2008，3，3： | 0 |
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