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| 1 | Special Section:SARS-CoV-2 Preliminary evidence from a multicenter prospective observational study of the safety and efficacy of chloroquine for the treatment of COVID-19显示文摘Effective therapies are urgently needed for the SARS-CoV-2 pandemic.Chloroquine has been proved to have antiviral effect against coronavirus in vitro.In this study,we aimed to assess the efficacy and safety of chloroquine with different doses in COVID-19.In this multicenter prospective observational study,we enrolled patients older than 18 years old with confirmed SARS-CoV-2 infection excluding critical cases from 12 hospitals in Guangdong and Hubei Provinces.Eligible patients received chloroquinephosphate s00 mg,orally,once(half dose)or twice(full dose)daily.Patients treated with non-chloroquine therapy were included as historical controls.The primary endpoint is the time to undetectable viral RNA.Secondary outcomes include the proportion of patients with undetectable viral RNA by day 10 and 14,hospitalization time,duration of fever,and adverse events.A total of 197 patients completed chloroquine treatment,and 176patients were included as historical controls.The median time to achieve an undetectable viral RNA was shorter in chloroquine than in non-chloroquine(absolute difference in medians-6.0 days;95%CI-6.0 to—4.0).The duration of fever is shorter in chloroquine(geometric mean ratio 0.6;95%CIO.5 to 0.8).No serious adverse events were observed in the chloroquine group.Patients treated with half dose experienced lower rate of adverse events than with full dose.Although randomized trials are needed for further evaluation,this study provides evidence for safety and efficacy of chloroquine in COVID-19 and suggests that chloroquine can be a cost-effective therapy for combating the COVID-19 pandemic. | Mingxing Huang Man Li Fei Xiao Pengfei Pang Jiabi Liang Tiantian Tang Shaoxuan Liu Binghui Chen Jingxian Shu Yingying You Yang Li Meiwen Tang Jianhui Zhou Guanmin Jiang Jingfen Xiang Wenxin Hong Songmei He Zhaoqin Wang Jianhua Feng Changqing Lin Yinong Ye Zhilong Wu Yaocai Li Bei Zhong Ruilin Sun Zhongsi Hong Jing Liu Huili Chen Xiaohua Wang Zhonghe Li Duanqing Pei Lin Tian Jinyu Xia Shanping Jiang Nanshan Zhong Hong Shan | 2020 | National Science Review2020，7，9： | 20 |
| 2 | Characterization and Evaluation of OsLCT1 and OsN ramp5 Mutants Generated Through CRISPR/Cas9-Mediated Mutagenesis for Breeding Low Cd Rice显示文摘To explore how rice(Oryza sativa L.) can be safely produced in Cd-polluted soil, OsLCT1 and OsNramp5 mutant lines were generated by CRISPR/Cas9-mediated mutagenesis. One of OsLCT1 mutant(lct1×1) and two of OsNramp5 mutants(nramp5×7 and nramp5×9) were evaluated for grain Cd accumulation and agronomic performances. In paddy field soil containing approximately 0.9 mg/kg Cd, lct1×1 grains contained approximately 40%(0.17 mg/kg) of the Cd concentration of the wild type parental line, less than the China National Food Safety Standard(0.20 mg/kg). Both OsNramp5 mutants showed low grain Cd accumulation(< 0.06 mg/kg) in the paddy(approximately 0.9 mg/kg Cd) or in pots in soil spiked with 2 mg/kg Cd. However, only nramp5×7 showed normal growth and yield, whereas the growth of nramp5×9 was severely impaired. The study showed that lct1×1 could be used to produce rice grains safe for human consumption in lightly contaminated paddy soils and nramp5×7 used in soils contaminated by much higher levels of Cd. | LIU Songmei JIANG Jie LIU Yang MENG Jun XU Shouling TAN Yuanyuan LI Youfa SHU Qingyao HUANG Jianzhong | 2019 | Rice science2019，26，2： | 15 |
| 3 | Effect of temperature on corrosion behavior of 3003 aluminum alloy in ethylene glycol–water solution显示文摘The effect of temperature on the corrosion behavior of 3003 aluminum alloy in ethylene glycol–water solution was investigated by potentiodynamic polarization and electrochemical impedance spectroscopy(EIS) techniques. The surface characterization was observed and determined by scanning electron microscopy(SEM), atomic force microscopy(AFM) and energy dispersive spectrometer(EDS). The results demonstrate that the anodic aluminum dissolution and the cathodic oxygen reduction were accelerated by the increased temperature. However, as temperature was over60 °C, the solubility and concentration of oxygen decreased, resulting in the inhibition of cathodic reaction. The cathodic reaction rate of 3003 aluminum alloy rose to the maximum at 60 °C. The Warburg impedance in Nyquist diagram diminished and then was replaced by a negative capacitance caused by the absorption of intermediate corrosion product on electrode. On the other hand,after potentiodynamic measurements, 3003 aluminum alloy suffered pitting corrosion. The dissolution of aluminum alloy around secondary phase particles expanded both horizontally and vertically. | Chen Xin Tian Wenming Li Songmei Yu Mei Liu Jianhua | 2016 | Chinese Journal of Aeronautics2016，29，4： | 14 |
| 4 | High Accurate Interpolation of NURBS Tool Path for CNC Machine Tools显示文摘Feedrate fluctuation caused by approximation errors of interpolation methods has great effects on machining quality in NURBS interpolation, but few methods can efficiently eliminate or reduce it to a satisfying level without sacrificing the computing efficiency at present. In order to solve this problem, a high accurate interpolation method for NURBS tool path is proposed. The proposed method can efficiently reduce the feedrate fluctuation by forming a quartic equation with respect to the curve parameter increment, which can be efficiently solved by analytic methods in real-time. Theoretically, the proposed method can totally eliminate the feedrate fluctuation for any 2nd degree NURBS curves and can interpolate 3rd degree NURBS curves with minimal feedrate fluctuation.Moreover, a smooth feedrate planning algorithm is also proposed to generate smooth tool motion with considering multiple constraints and scheduling errors by an efficient planning strategy. Experiments are conducted to verify the feasibility and applicability of the proposed method. This research presents a novel NURBS interpolation method with not only high accuracy but also satisfying computing efficiency. | LIU Qiang LIU Huan YUAN Songmei | 2016 | Chinese Journal of Mechanical Engineering2016，29，5： | 10 |
| 5 | Corrosion behavior of an Al-6Mg-Sc-Zr alloy显示文摘The corrosion behavior of an Al-6Mg-Sc-Zr alloy was studied and compared with that of an Al-6Mg-Zr alloy.The addition of scandium into the Al-6Mg-Zr alloy reduced the susceptibility to exfoliation corrosion. By using the constantload tensile method in a 3.5 wt.% NaCl solution, the resistance to SCC of the Al-6Mg-Sc-Zr alloy was higher than that ofthe Al-6Mg-Zr alloy. When the specimens were not applied with an anodic current, the Al-6Mg-Sc-Zr alloy was resistanceto SCC and no brittle cracking was found on the fracture surface. When an anodic current was applied, the Al-6Mg-Sc-Zralloy specimens failed as a result of accelerated corrosion rather than SCC. It was believed that the addition of scandium re-sulted in (Al3Sc, Zr) particles that greatly refined grains and promoted the formation of homogeneous discontinuous distri-bution of β-phase in the alloy base, which much contributed to good corrosion resistance of the Al-6Mg-Sc-Zr alloy. | XU Guangxin TAO Binwu LIU Jianhua LI Songmei | 2005 | Rare Metals2005，24，3： | 8 |
| 6 | Prediction of Dynamic Cutting Force and Regenerative Chatter Stability in Inserted Cutters Milling显示文摘Currently, the modeling of cutting process mainly focuses on two aspects: one is the setup of the universal cutting force model that can be adapted to a broader cutting condition; the other is the setup of the exact cutting force model that can accurately reflect a true cutting process. However, there is little research on the prediction of chatter stablity in milling. Based on the generalized mathematical model of inserted cutters introduced by ENGIN, an improved geometrical, mechanical and dynamic model for the vast variety of inserted cutters widely used in engineering applications is presented, in which the average directional cutting force coefficients are obtained by means of a numerical approach, thus leading to an analytical determination of stability lobes diagram (SLD) on the axial depth of cut. A new kind of SLD on the radial depth of cut is also created to satisfy the special requirement of inserted cutter milling. The corresponding algorithms used for predicting cutting forces, vibrations, dimensional surface finish and stability lobes in inserted cutter milling under different cutting conditions are put forward. Thereafter, a dynamic simulation module of inserted cutter milling is implemented by using hybrid program of Matlab with Visual Basic. Verification tests are conducted on a vertical machine center for Aluminum alloy LC4 by using two different types of inserted cutters, and the effectiveness of the model and the algorithm is verified by the good agreement of simulation result with that of cutting tests under different cutting conditions. The proposed model can predict the cutting process accurately under a variety of cutting conditions, and a high efficient and chatter-free milling operation can be achieved by a cutting condition optimization in industry applications. | LI Zhongqun LIU Qiang YUAN Songmei HUANG Kaisheng | 2013 | Chinese Journal of Mechanical Engineering2013，26，3： | 8 |
| 7 | Preparation and characterization of the TiO_2-V_2O_5 photocatalyst with visible-light activity显示文摘Visible-light responsive TiO2-V2O5 catalyst was prepared using a binary sol-gel and in-situ intercalation method. The TiO2 sol and V2O5 sol were mixed to disperse the V2O5 species in the TiO2 phase at molecular level. The binary sol was then intercalated into interspaces of polyaniline (PANI) by means of in-situ polymerization of aniline. Conglomeration of the TiO2-V2O5 clusters during the calcination process was avoided because of the wrap of polyaniline. The surface mor- phology, the crystal phases, the structure, and the absorption spectra of (PANI)n/TiO2-V2O5 and the composite catalyst were studied using SEM, XRD, FT-IR, and UV-Vis. The photoactivity of the prepared catalyst under UV and visible light irra- diation were evaluated by decolorization of methylene blue (MB) solution. The results showed that the composite catalyst displayed a homogeneous anatase phase, and the vanadium pentoxide species was highly dispersed in the TiO2 phase. The composite catalyst responded to visible light because of the narrowed band gap. In this study, the catalyst with the sol vol- ume ratio of TiO2 : V2O5 = 10:1 presented the best photocatalytic activity. | LIU Jianhua YANG Rong LI Songmei | 2006 | Rare Metals2006，25，6： | 7 |
| 8 | Preparation of bimodal grain size 7075 aviation aluminum alloys and their corrosion properties显示文摘The bimodal grain size metals show improved strength and ductility compared to traditional metals; however, their corrosion properties are unknown. In order to evaluate the corrosion properties of these metals, the bimodal grain size 7075 aviation aluminum alloys containing different ratios of coarse(100 μm in diameter) and fine(10 μm in diameter) grains were prepared by spark plasma sintering(SPS). The effects of grain size as well as the mixture degree of coarse and fine grains on general corrosion were estimated by immersion tests, electrochemical measurements and complementary techniques such as scanning electron microscope(SEM) and transmission electron microscope-energy disperse spectroscopy(TEM-EDS). The results show that, compared to fine grains, the coarse grains have a faster dissolution rate in acidic NaCl solution due to the bigger size,higher alloying elements content and larger area fraction of second phases in them. In coarse grains,the hydrogen ions have a faster reduction rate on cathodic second phases, therefore promoting the corrosion propagation. The mixture of coarse and fine grains also increases the electrochemical heterogeneity of alloys in micro-scale, and thus the increased mixture degree of these grains in metal matrix accelerates the corrosion rate of alloys in acidic NaCl solution. | Wenming TIAN Songmei LI Jianhua LIU Mei YU Yujie DU | 2017 | Chinese Journal of Aeronautics2017，30，5： | 7 |
| 9 | Fabrication and characterization of highly ordered Ni_(0.5)Zn_(0.5)Fe_2O_4 nanowire/tube arrays by sol-gel template method显示文摘Highly ordered nanowire/tube arrays of Ni0.5Zn0.5Fe2O4 were fabricated by the sol-gel method in the pores of anodic alu- mina membrane (AAM). Whether nanowires or nanotubes were fabricated depends on immersion time. The immersion time was 15-40 s for nanotubes and over 60 s for nanowires. The topography and crystalline structure of the nanowire arrays were character- ized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD). It was found that the length and diameter of the Ni0.5Zn0.5Fe2O4 nanowires are related to the thickness of the AAM and the diameter of the pores. The results indicated that the Ni0.5Zn0.5Fe2O4 nanowires are uniform and parallel to each other. | Mei Yu Jianhua Liu Songmei Li | 2007 | Journal of University of Science and Technology Beijing2007，14，5： | 6 |
| 10 | Preparation and characterization of high photoactive TiO_2 catalyst using the UV irradiation-induced sol-gel method显示文摘High photoactive TiO2 catalyst was prepared using the sol-gel method through UV irradiation during the formation stage of nuclei. The surface morphology and microstructure of the prepared catalyst were characterized using scanning electron microscopy (SEM), X-ray diffraction patterns (XRD), and Fourier transform infrared spectroscopy (FT-IR). The photoactivity was evaluated by the degradation of methylene blue. The results show that the photocatalysis of the prepared catalyst is higher than that of conventional heat-treated particles. The higher photoactivity is a combined result of favorable microstructure, appropriate hydroxyl groups, and active sites of Ti3+ ions on the surface of TiO2. It is concluded that the ultraviolet irradiation-induced sol-gel method is an effective method to enhance the photocatalysis of TiO2. | Jianhua Liu Rong Yang Songmei Li | 2006 | Journal of University of Science and Technology Beijing2006，13，4： | 4 |
| 11 | Influence of Minimum Quantity Lubrication Parameters on Tool Wear and Surface Roughness in Milling of Forged Steel显示文摘The minimum quantity of lubrication(MQL) technique is becoming increasingly more popular due to the safety of environment.Moreover,MQL technique not only leads to economical benefits by way of saving lubricant costs but also presents better machinability.However,the effect of MQL parameters on machining is still not clear,which needs to be overcome.In this paper,the effect of different modes of lubrication,i.e.,conventional way using flushing,dry cutting and using the minimum quantity lubrication(MQL) technique on the machinability in end milling of a forged steel(50CrMnMo),is investigated.The influence of MQL parameters on tool wear and surface roughness is also discussed.MQL parameters include nozzle direction in relation to feed direction,nozzle elevation angle,distance from the nozzle tip to the cutting zone,lubricant flow rate and air pressure.The investigation results show that MQL technique lowers the tool wear and surface roughness values compared with that of conventional flood cutting fluid supply and dry cutting conditions.Based on the investigations of chip morphology and color,MQL technique reduces the cutting temperature to some extent.The relative nozzle-feed position at 120°,the angle elevation of 60° and distance from nozzle tip to cutting zone at 20 mm provide the prolonged tool life and reduced surface roughness values.This fact is due to the oil mists can penetrate in the inner zones of the tool edges in a very efficient way.Improvement in tool life and surface finish could be achieved utilizing higher oil flow rate and higher compressed air pressure.Moreover,oil flow rate increased from 43.8 mL?h to 58.4 mL?h leads to a small decrease of flank wear,but it is not very significant.The results obtained in this paper can be used to determine optimal conditions for milling of forged steel under MQL conditions. | YAN Lutao YUAN Songmei LIU Qiang | 2012 | Chinese Journal of Mechanical Engineering2012，25，3： | 4 |
| 12 | SCC evaluation of a 2297 Al-Li alloy rolled plate using the slow-strain rate technique显示文摘The stress corrosion cracking(SCC) susceptibility of 2297 Al-Li alloy in 1 M Na Cl +0.01 M H2O2 solution(CP solution) and 1 M NaCl + 0.01 M H2O2+ 0.6 M Na2SO4 solution(CPS solution) was investigated by slow-strain rate tests at various strain rates ranging from 10-5s(-1) to 10-7s-1. The roles of H2O2 and SO42-in the corrosion process were estimated by potentiodynamic polarization and electrochemical impedance spectroscopy. 2297 Al-Li alloy does not fracture ascribed to SCC in CP solution, while it undergoes SCC in CPS solution. In CPS solution,with a decreasing strain rate from 10-5s(-1) to 10-7s-1, the SCC susceptibility firstly rises and then declines exhibiting a peak value at a strain rate of 10-6s-1. H2O2 promotes the active dissolution while SO42- lowers the corrosion rate. The SCC fracture is associated with a decline in the dissolution rate of the crack tip by SO42-, which leads to stress concentration. In CPS solution, a reduction in the local dissolution rate of the crack tip leads to stress concentration, resulting in SCC fracture.As the preferred initiation site for a crack, pits also show a noteworthy effect on SCC of 2297 Al-Li alloy. | Kuo ZHAO Jianhua LIU Mei YU Songmei LI | 2019 | Chinese Journal of Aeronautics2019，32，11： | 3 |
| 13 | N-terminal domains of ARC1 are essential for interaction with the N-terminal region of Exo70A1 in transducing self-incompatibility of Brassica oleracea显示文摘自我障碍(SI ) 是一个重要交配系统阻止使近交并且支持 outcrossing。ARC1 和 Exo70A1 在 Brassica SI 发信号作为 S 地点受体 kinase 和戏保守人士角色的下游的目标工作。基于顺序相同, Exo70A1 被划分成四子域:白氨酸拉链(列伊 128-Leu149), hypervariable 区域(重量的单位 172-Leu197), 相扑修正主题(Glu 260-Ile275), 和 pfamExo70 领域(他的 271-Phe627) 。ARC1 如下包含四个领域:白氨酸拉链(列伊 116-Leu137), 卷卷领域(Thr 210-Val236),U 盒子(毒蛇 282-Trp347) 主题,和手臂(翼 415-Thr611) 领域。生物信息学分析,酵母二混血儿的屏蔽和 ARC1 116-236 的白氨酸拉链和卷卷主题为和 Exo70A1 的相互作用被要求的下拉试金表演,当手臂主题的增加导致和 Exo70A1 的相互作用的损失时。同时,没有任何域的 Exo70A1 的N终端与 ARC1 显示出一个弱相互作用,并且 LacZ 表示的水平与白氨酸拉链的增加增加并且与 hypervariable 区域和相扑修正主题到达最大的值,显示那个 hypervariable 区域和 Exo70A1 172-275的相扑修正主题为有 ARC1 的绑定主要负责,而 pfamExo70 领域为 ARC1 有很少亲密关系。位于 Exo70A1 hypervariable 区域的 Lys 181 可以是调停的 ubiquitination 地点在 ARC1 和 Exo70A1 之间的相互作用。因此,两有 ARC1 116-236, 和 hypervariable 区域和 Exo70A1 172-275 的相扑修正主题的卷卷结构的白氨酸拉链是在 ARC1 和 Exo70A1 之间的核心相互作用领域。影响这些核心领域的任何因素将是调停的 ARC1 的管理者在自我不兼容的系统的 ubiquitin 降级。 | Songmei Shi Qiguo Gao Jing Zeng Xiaohuan Liu Quanming Pu Guixi Liu Hecui Zhang Xiaohong Yang Liquan Zhu | 2016 | Acta Biochimica et Biophysica Sinica2016，48，9： | 3 |
| 14 | Effect of sulphate-reducing bacteria on the electrochemical impedance spectroscopy characteristics of 1Cr18Ni9Ti显示文摘The electrochemical characteristics of 1Cr18Ni9Ti in sulphate-reducing bacteria (SRB) solutions and the biofilm of SRB on the surface of the 1Cr18Ni9Ti electrode were studied by electrochemical, microbiological, and surface analysis methods. Electro- chemical impedance spectroscopy (EIS) of 1Cr18Ni9Ti was measured in the solutions with and without SRB at the culture time of 2, 4, 8 d, respectively. The measurement used two test methods, the nonimmersion electrode method and the immersion electrode method. It was found that the polarization resistance (Rp) of 1Cr18Ni9Ti in the solutions without SRB is the greatest for each test method. When using the nonimmersion electrode method, Rp shifts negatively at first and then positively, and the time constant is only one. Although using the immersion electrode method, the Rp shifts positively at first and then negatively, and the time constant also changes when the biofilm forms. The biofilm observed through SEM is with pores. It was demonstrated that SRB has acceler- ated corrosion action on 1Cr18Ni9Ti. The protection effect of the biofilm on the electrode depends on the compact degree of the film. | Jianhua Liu Xin Liang Songmei Li | 2007 | Journal of University of Science and Technology Beijing2007，14，5： | 3 |
| 15 | SCC investigation of low alloy ultra-high strength steel 30CrMnSiNi2A in 3.5wt% NaCl solution by slow strain rate technique显示文摘To evaluate stress corrosion cracking(SCC) mechanism of low alloy ultra-high strength steel 30CrMnSiNi2 A in environment containing NaCl, SCC behavior of the steel in 3.5wt% NaCl solution is investigated by slow strain rate technique(SSRT) with various strain rates and applied potentials, surface analysis technique, and electrochemical measurements. SCC susceptibility of the steel increases rapidly with strain rate decreasing from 1 · 10 5s 1to 5 · 10 7s 1, and becomes stable when strain rate is lower than 5 · 10 7s 1. SCC propagation of the steel in the solution at open circuit potential(OCP) needs sufficient hydrogen which is supplied at a certain strain rate.Fracture surface at OCP has similar characteristics with that at cathodic polarization 1000 mVSCE, which presents characteristic fractography of hydrogen induced cracking(HIC).All of these indicate that SCC behavior of the steel in the solution at OCP is mainly controlled by HIC rather than anodic dissolution(AD). | Liu Jianhua Guo Qiang Yu Mei Li Songmei | 2014 | Chinese Journal of Aeronautics2014，27，5： | 3 |
| 16 | Preparation and characterization of highly ordered NiO nanowire arrays by sol-gel template method显示文摘Highly ordered nickel monoxide (NiO) nanowire arrays were fabricated by sol-gel synthesis within the pores of anodic alu-mina membrane (AAM). Scanning electron microscopy (SEM), high resolution transmission electron microscopy (HRTEM) and X-raydiffraction (XRD) were used to characterize the topography and crystalloid structure of NiO nanowire arrays. The length and diameterof the NiO nanowires depended on the thickness of the AAM and the diameter of the pores. The results indicated that the NiOnanowires were uniformly assembled into the ordered nanopores of the AAM and paralleled to each other. Nickel monoxide nanotubeswere also fabricated with the same method by changing the immersing time. This new method to prepare NiO nanowire arrays may beimportant from gas sensors to various engineering materials. | Mei Yu Jianhua Liu Songmei Li | 2006 | Journal of University of Science and Technology Beijing2006，13，2： | 3 |
| 17 | Effects of cerium salts on corrosion behaviors of Si–Zr hybrid sol–gel coatings显示文摘The present work examines the effects of cerium salts on corrosion behaviors of Si–Zr hybrid sol–gel coatings. The Si–Zr hybrid sol–gel coatings on a 2A12 aluminum substrate were prepared through hydrolysis and condensation of glycidoxypropyl-trimethoxy-silane(GTMS) and zirconium(IV) n-propoxide(TPOZ). Used as inhibitors for corrosion, three types of cerium salts(Ce(NO_3)_3, CeCl_3, and Ce(CH_3COO)_3) were doped into the sol–gel coatings. Fourier transform infrared(FTIR) and scanning electron microscopy(SEM) were employed to investigate the structures and morphologies of various coatings, and the corrosion resistances of the coatings were evaluated by electrochemical methods and neutral salt spray tests. Experimental results indicate that the addition of cerium salts can hinder the process of corrosion due to their self-healing abilities. Furthermore, the sol–gel coating doped with Ce(CH_3COO)_3 has the best corrosion resistance because of the promotions of hydrolysis and condensation provided by CH_3COO. | Yu Mei Liu Yuxing Liu Jianhua Li Songmei Xue Bing Zhang You Yin Xiaolin | 2015 | Chinese Journal of Aeronautics2015，28，2： | 2 |
| 18 | Programmable DNA-responsive microchip for the capture and release of circulating tumor cells by nucleic acid hybridization显示文摘 | Shan Guo Haiyan Huang Xujing Deng Yuqi Chen Zhuoran Jiang Min Xie Songmei Liu Weihua Huang Xiang Zhou | 2018 | Nano Research2018，11，5： | 2 |
| 19 | Effects of dry/wet ratio and pre-immersion on stress corrosion cracking of 7050-T7451 aluminum alloy under wet-dry cyclic conditions显示文摘The stress corrosion cracking(SCC) behavior and mechanism of 7050-T7451 aluminum alloy under wet-dry cyclic conditions were investigated. Slow strain rate tests(SSRTs) and electrochemical tests were used to study the effects of dry/wet ratio(DWR) and pre-immersion on SCC.Fracture and side surface characterizations were observed by scanning electron microscopy(SEM).The results demonstrate that SCC susceptibility decreases with an increase of the DWR. With an increase of the pre-immersion time, both continuous pre-immersion(CP) and wet-dry cyclic preimmersion(WDP) samples are more sensitive to SCC, and the cracking mode in the SCC fracture region is intergranular. Furthermore, the effect of WDP on SCC is greater than that of CP when the total time immersed in solution before an SSRT is the same with each other. In fact, each single wetdry cycle can be divided into three processes with respect to the change of solution on samples' surface. Volatilization of water on the surface results in an increase in solute concentration, thus accelerating corrosion. | Mei YU Hang ZU Kuo ZHAO Jianhua LIU Songmei LI | 2018 | Chinese Journal of Aeronautics2018，31，11： | 2 |
| 20 | Identification and characterization of BoPUB3: a novel interaction protein with S-locus receptor kinase in Brassica oleracea L.显示文摘Armadillo repeat containing 1 (ARC1) is phosphorylated by S-locus receptor kinase (SRK) and functions as a positive regulator in self-incompatibility response of Brassica. However, ARC1 only causes partial breakdown of the self-incompatibility response, and other SRK downstream factors may also participate in the self-incompatibility signaling pathway. In the present study, to search for SRK downstream targets, a plant U-box protein 3 (BoPUB3) was identified from the stigma of Brassica oleracea L. BoPUB3 was highly expressed in the stigma, and its expression was increased with the stigma development and reached to the highest level in the mature-stage stigma. BoPUB3, a 76.8-kDa protein with 697 amino acids, is a member of the PUB-ARM family and contains three domain characteristics of BoARC1, including a U-box N-terminal domain, a U-box motif, and a C-terminal arm repeat domain. The phylogenic tree showed that BoPUB3 was close to BoARC1. The synteny analysis revealed that B. oleracea chromosomal region containing BoPUB3 had high synteny with the Arabidopsis thaliana chromosomal region containing AtPUB3 (At3G54790). In addition, the subcellular localization analysis showed that BoPUB3 primarily localized in the plasma membrane and also in the cytoplasm. The combination of the yeast two-hybrid and in vitro binding assay showed that both BoPUB3 and BoARC1 could interact with SRK kinase domain, and SRK showed much higher level of β-galactosidase activity in its interaction with BoPUB3 than with BoARC1. These results implied that BoPUB3 is a novel interactor with SRK, which lays a basis for further research on whether PUB3 participates in the self-incompatibility signaling pathway. | Songmei Shi Qiguo Gao Tonghong Zuo Zhenze Lei Quanming Pu Yukui Wang Guixi Liu Xinhua He Xuesong Ren Liquan Zhu | 2019 | Acta Biochimica et Biophysica Sinica2019，51，7： | 2 |
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