|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Key residues of the receptor binding motif in the spike protein of SARS-CoV-2 that interact with ACE2 and neutralizing antibodies显示文摘Coronavirus disease 2019(COVID-19),caused by the novel human coronavirus SARS-CoV-2,is currently a major threat to public health worldwide.The viral spike protein binds the host receptor angiotensin-converting enzyme 2(ACE2)via the receptor-binding domain(RBD),and thus is believed to be a major target to block viral entry.Both SARS-CoV-2 and SARS-CoV share this mechanism.Here we functionally analyzed the key amino acid residues located within receptor binding motif of RBD that may interact with human ACE2 and available neutralizing antibodies.The in vivo experiments showed that immunization with either the SARS-CoV RBD or SARS-CoV-2 RBD was able to induce strong clade-specific neutralizing antibodies in mice;however,the cross-neutralizing activity was much weaker,indicating that there are distinct antigenic features in the RBDs of the two viruses.This finding was confirmed with the available neutralizing monoclonal antibodies against SARS-CoV or SARS-CoV-2.It is worth noting that a newly developed SARS-CoV-2 human antibody,HA001,was able to neutralize SARS-CoV-2,but failed to recognize SARS-CoV.Moreover,the potential epitope residues of HA001 were identified as A475 and F486 in the SARS-CoV-2 RBD,representing new binding sites for neutralizing antibodies.Overall,our study has revealed the presence of different key epitopes between SARS-CoV and SARSCoV-2,which indicates the necessity to develop new prophylactic vaccine and antibody drugs for specific control of the COVID-19 pandemic although the available agents obtained from the SARS-CoV study are unneglectable. | Chunyan Yi Xiaoyu Sun Jing Ye Longfei Ding Meiqin Liu Zhuo Yang Xiao Lu Yaguang Zhang Liyang Ma Wangpeng Gu Aidong Qu Jianqing Xu Zhengli Shi Zhiyang Ling Bing Sun | 2020 | Cellular & Molecular Immunology2020，17，6： | 13 |
| 2 | Simultaneous determination of ivabradine and N-desmethylivabradine in human plasma and urine using a LC-MS/MS method: application to a pharmacokinetic study显示文摘A sensitive and specific liquid-chromatography tandem mass spectrometry(LC-MS/MS)assay has been developed and validated for the simultaneous quantification of ivabradine and its active metabolite N-desmethylivabradine in human plasma and urine.The assay employed a single liquid–liquid extraction of the analytes from plasma and urine samples,and diazepam was used as internal standard(IS).The chromatographic separation was achieved on a Diamonsil C18 column(150 mm4.6 mm,5 mm,Dikma)using a mixture of methanol and aqueous 5 mM ammonium acetate buffer containing 0.2%formic acid(80:20,v/v)as mobile phase.The assay for ivabradine and N-desmethylivabradine in plasma showed good linearity(r≥0.99)over the ranges 0.1013–101.3 ng/mL and 0.085–25.5 ng/mL,respectively.The assay for ivabradine and N-desmethylivabradine in urine showed good linearity(r≥0.99)over the ranges 10.13–6078 ng/mL and 8.5–850 ng/mL,respectively.The intra-and inter-day accuracy and precision values were found to be within the assay variability limits(RSD<15%)in accordance with FDA guidelines.The methods were successfully used for evaluating the pharmacokinetic properties of ivabradine and N-desmethylivabradine in human plasma and urine in Chinese healthy volunteers. | Chengtao Lu Yanyan Jia Jing Yang Xin Jin Ying Songa Wenxing Liu Yi Ding Xiaoli Sun Aidong Wen | 2012 | Acta Pharmaceutica Sinica B2012，2，2： | 3 |
| 3 | Safety and efficacy of meplazumab in healthy volunteers and COVID-19 patients: a randomized phase 1 and an exploratory phase 2 trial显示文摘Recent evidence suggests that CD147 serves as a novel receptor for severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection.Blocking CD147 via anti-CD147 antibody could suppress the in vitro SARS-CoV-2 replication.Meplazumab is a humanized anti-CD147 IgG_(2) monoclonal antibody,which may effectively prevent SARS-CoV-2 infection in coronavirus disease 2019(COVID-19)patients.Here,we conducted a randomized,double-blinded,placebo-controlled phase 1 trial to evaluate the safety,tolerability,and pharmacokinetics of meplazumab in healthy subjects,and an open-labeled,concurrent controlled add-on exploratory phase 2 study to determine the efficacy in COVID-19 patients.In phase 1 study,59 subjects were enrolled and assigned to eight cohorts,and no serious treatment-emergent adverse event(TEAE)or TEAE grade≥3 was observed.The serum and peripheral blood Cmax and area under the curve showed non-linear pharmacokinetic characteristics.No obvious relation between the incidence or titer of positive anti-drug antibody and dosage was observed in each cohort.The biodistribution study indicated that meplazumab reached lung tissue and maintained>14 days stable with the lung tissue/cardiac blood-pool ratio ranging from 0.41 to 0.32.In the exploratory phase 2 study,17 COVID-19 patients were enrolled,and 11 hospitalized patients were involved as concurrent control.The meplazumab treatment significantly improved the discharged(P=0.005)and case severity(P=0.021),and reduced the time to virus negative(P=0.045)in comparison to the control group.These results show a sound safety and tolerance of meplazumab in healthy volunteers and suggest that meplazumab could accelerate the recovery of patients from COVID-19 pneumonia with a favorable safety profile. | Huijie Bian Zhao-Hui Zheng Ding Wei Aidong Wen Zheng Zhang Jian-Qi Lian Wen-Zhen Kang Chun-Qiu Hao Jing Wang Rong-Hua Xie Ke Dong Jie-Lai Xia Jin-Lin Miao Wen Kang Guoquan Li Di Zhang Mingru Zhang Xiu-Xuan Sun Likun Ding Kui Zhang Junfeng Jia Jin Ding Zhiqin Li Yanyan Jia Lin-Na Liu Zhe Zhang Zhao-Wei Gao Hong Du Na Yao Qing Wang Ke Wang Jie-Jie Geng Bin Wang Ting Guo Ruo Chen Yu-Meng Zhu Li-Juan Wang Qian He Rui-Rui Yao Ying Shi Xiang-Min Yang Jian-Sheng Zhou Yi-Nan Ma Ya-Tao Wang Xue Liang Fei Huo Zhe Wang Yang Zhang Xu Yang Ye Zhang Lu-Hua Gao Ling Wang Xiao-Chun Chen Hao Tang Shuang-Shuang Liu Qing-Yi Wang Zhi-Nan Chen Ping Zhu | 2021 | Signal Transduction and Targeted Therapy2021，6，6： | 2 |
| 4 | Some nutritional and functional properties of defatted wheat germ protein 显示文摘 | Yiqiang Ge Aidong Sun Yangying Ni Tongyi Cai | 2000 | Journal of Agriculture and Food chemistry2000，48，12： | 1 |
| 5 | Studies of the protective effect and antioxidant mechanism of blueberry anthocyanins in a CC14-induced liver injury model in mice 显示文摘 | jian Chen Hongnan Sun Aidong Sun | 2012 | Food Agric Immunol2012，23，4： | 1 |
| 6 | Some nutritional and functional properties of defatted wheat germ protein显示文摘 | Yiqiang Ge Aidong Sun Yangying Ni Tongyi Cai | 2000 | Journal of Agriculture and Food Chemistry2000，48，12： | 1 |
| 7 | Some nutritional and functional properties of defatted wheat germ protein显示文摘 | Ge Yiqiang Sun Aidong Ni Yangying | 2000 | Journal of Agriculture and Food Chemistry2000，48，12： | 1 |
| 8 | Application of Excel2002 incalculation of TCM dactylogram similarity显示文摘 | Miao Aidong Sun Dianjia | 2003 | Computer andPharmacy2003，27，1： | 1 |
| 9 | Some nutritional and functional properties of defatted wheat germ protein显示文摘 | Yiqiang Ge Aidong Sun Yangying Ni | 2000 | Journal of Agriculture and Food Chemistry2000，48，12： | 1 |
| 10 | Some Nutritional and Functional Properties of Defatted W heat Germ Protein 显示文摘 | Yiqiang Ge Aidong Sun Yangying Ni et a! | 2000 | Journal of Agricultureand Food Chemistry2000，48，12： | 1 |
| 11 | Some nutritional and functional properties of de-fatted wheat germ protein显示文摘 | Yiqiang Ge Aidong Sun Yangying Ni Tongyi Cai | 2000 | Journal of Agriculture and Food Chemistry2000，48，12： | 1 |
| 12 | Some nutritional and functional properties of defatted wheat germ protein显示文摘 | Yiqiang Ge Aidong Sun Yangying Ni | 2000 | Journal of Agriculture and Food Chemistry2000，48，12： | 1 |
| 13 | Some Nutritional and Functional Properties of Defatted Wheat Germ Protein显示文摘 | Yiqiang Ge Aidong Sun Yangying Ni etc | 2000 | Journal of Agriculture and Food Chemistry2000，48，12： | 1 |
| 14 | Some nutritional and functional properties of defatted wheat germ protein 显示文摘 | Yiqiang Ge Aidong Sun Yuanying Ni | 2000 | J Agric Food Chem2000，48，12： | 1 |
| 15 | Studies of the protective effect and antioxidant mechanism of blueberry anthocyanins in a CC14-induced liver injury model in mice显示文摘 | Jian Chen Hongnan Sun Aidong Sun | | 0，，04： | 1 |
| 16 | Some nutritional and functional properties of defatted wheat germ protein显示文摘 | Yiqiang Ge Aidong Sun Yangying Ni | 2000 | Journal of Agriculture and Food Chemistry2000，48，12： | 1 |
| 17 | Some nutritional and functional properties of defatted wheat germ protein显示文摘 | Yiqiang Ge Aidong Sun Yangying Ni Tongyi Cai | 2000 | Journal of Agriculture and Food chemistry2000，48，12： | 1 |
| 18 | Resistive Switching Properties and Failure Behaviors of(Pt, Cu)/Amorphous ZrO_(2)/Pt Sandwich Structures显示文摘The effect of Pt and Cu electrodes on the resistive switching properties and failure behaviors of amorphous ZrO_2 ?lms were investigated. Compared with Cu/ZrO_2/Pt structures, the Pt/ZrO_2/Pt structures exhibit better resistive switching properties such as the higher resistance ratio of OFF/ON states, the longer switching cycles and narrow distribution of OFF state resistance(Roff). The switching mechanism in the Pt/ZrO_2/Pt structure can be attributed to the formation and rupture of oxygen vacancy ?laments; while in the Cu/Zr O2/Pt structure, there exist both oxygen vacancy ?laments and Cu ?laments. The formation of Cu?laments is related to the redox reaction of Cu electrode under the applied voltage. The inhomogeneous dispersive injection of Cu ions results in the dispersive Roff and signi?cant decrease of operate voltage.Schematic diagrams of the formation of conductive ?laments and the failure mechanism in the Cu/ZrO_2/Pt structures are also proposed. | Haifa Zhai Jizhou Kong Jien Yang Jing Xu Qingran Xu Hongchen Sun Aidong Li Di Wu | 2016 | Journal of Materials Science & Technology2016，32，7： | 0 |
| 19 | Cultivation of Hippeastrum vittatum Embryogenic Calli and Their Sensitivity to Antibiotics显示文摘In this study,an embryogenic callus induction and proliferation system for Hippeastrum vittatum was established,with the tender bulbs as explants. And then the sensitivity of the explants and calli to kanamycin and hygromycin was evaluated. The results suggested that the embryogenic calli were induced from tender bulbs and cultured in Murashige and Skoog( MS) medium supplemented with 0. 5 mg/L N-phenyl-N'-1,2,3-thiadiazol-5-ylurea( TDZ),1. 0 mg/L 2,4-dichlorophenoxyacetic acid( 2,4-D),and 30 g/L sucrose( pH5. 8) in the dark at 25 ± 1 ℃. Further study of the influence of kanamycin and hygromycin on callus induction and multiplication showed that,the lethal doses of kanamycin and hygromycin to bulb explants were 100 and 30 mg/L,respectively. All explants of H. vittatum died on the medium supplemented with 100 mg/L kanamycin or 30 mg/L hygromycin at the induction stage,and callus proliferation was completely inhibited by 100 mg/L kanamycin or 25 mg/L hygromycin,and all the calli died at last. These results will provide important reference for further studies of transgenic H. vittatum. | Bo YU Min LIU Yu ZHU Aidong ZHONG Lili HUANG Dongxiang ZHU Chunhui LI Genfa ZHU Yingbo SUN | 2018 | Agricultural Biotechnology2018，7，1： | 0 |
| 20 | A Trust-Based Hierarchical Consensus Mechanism for Consortium Blockchain in Smart Grid显示文摘As the smart grid develops rapidly,abundant connected devices offer various trading data.This raises higher requirements for secure and effective data storage.Traditional centralized data management does not meet the above requirements.Currently,smart grid with conventional consortium blockchain can solve the above issues.However,in the face of a large number of nodes,existing consensus algorithms often perform poorly in terms of efficiency and throughput.In this paper,we propose a trust-based hierarchical consensus mechanism(THCM)to solve this problem.Firstly,we design a hierarchical mechanism to improve the efficiency and throughput.Then,intra-layer nodes use an improved Raft consensus algorithm and inter-layer nodes use the Byzantine Fault Tolerance algorithm.Thirdly,we propose a trust evaluation method to improve the election process of Raft.Finally,we implement a prototype system to evaluate the performance of THCM.The results demonstrate that the consensus efficiency is improved by 19.8%,the throughput is improved by 12.34%,and the storage is reduced by 37.9%. | Xingguo Jiang Aidong Sun Yan Sun Hong Luo Mohsen Guizani | 2023 | Tsinghua Science and Technology2023，28，1： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员