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| 1 | Mesenteric lymph reperfusion may exacerbate brain injury in a rat model of superior mesenteric artery occlusion shock显示文摘BACKGROUND:The intestinal lymphatic pathway and intestinal ischemia/reperfusion are mainly involved in mesenteric lymph duct ligation or drainage;moreover,intervention by reducing the lymph liquid reflux might relieve lung and other organ dysfunction induced by intestinal ischemia/reperfusion;however,research addressing mesenteric lymph reperfusion (MLR) and brain injury has not yet to be reported.OBJECTIVE:To observe the effect of MLR on brain tissue in a rat model of superior mesenteric artery occlusion (SMAO) shock,and to explore the molecular mechanism of MLR.DESIGN,TIME AND SETTING:A randomized,controlled,animal experiment at a neuro-pathophysiology level was performed at the Institute of Microcirculation,Hebei North University;Department of Pathophysiology,Basic Medical College;Department of Pathology,the First Hospital of Hebei North University between December 2007 and March 2009.MATERIALS:Adenosine triphosphate (ATP) standard was provided by the National Institute for the Control of Pharmaceutical and Biological Products;lactic acid (LA),superoxide dismutase (SOD),malonaldehyde (MDA),nitrogen monoxidum (NO),nitric oxide synthase (NOS),myeloperoxidase (MPO) and ATPase assay kits were provided by Nanjing Jiancheng Bioengineering Institute,China.METHODS:A total of 24 male Wistar rats were randomly divided into four groups.In the sham-surgery group (n=6),both the mesenteric lymph duct and the superior mesenteric artery were not blocked;in the MLR group (n=6),the mesenteric lymph duct was occluded for 1 hour followed by 2-hour reperfusion;in the SMAO group (n=6),the superior mesenteric artery was occluded for 1 hour followed by 2-hour reperfusion;in the MLR + SMAO group (n=6),both the mesenteric lymph duct and superior mesenteric artery were occluded for 1 hour followed by 2-hour reperfusion.MAIN OUTCOME MEASURES:Mean arterial blood pressure prior to and following ischemia/reperfusion;brain tissue morphology levels of LA,MDA,SOD,NO,NOS,MPO,ATPase and ATP following reperfusion.RESULTS:MLR did not cause changes in mean arterial blood pressure,brain tissue morphology,LA,MDA,NO,ATP,SOD,NOS,MPO and ATPase.However,SMAO caused a rapid decrease and gradual increase of mean arterial blood pressure.Neuronal necrosis,degeneration and swelling were observed in brain tissue.Contents of MDA,NO,LA and ATP as well as activities of NOS and MPO were significantly increased (P < 0.05),but activities of SOD and Na+-K+-ATPase were significantly decreased (P < 0.05).MLR aggravated neuronal damage in a rat model of SMAO shock.Following MLR,mean arterial blood pressure was significantly decreased (P < 0.05),contents of MDA and NO as well as activities of NOS and MPO were significantly increased (P < 0.05),but activities of Ca2+-ATPase,Mg2+-ATPase and Ca2+-Mg2+-ATPase as well as ATP content were significantly decreased (P < 0.05).CONCLUSION:MLR aggravates brain injury in a rat model of SMAO shock,which correlates with oxygen-derived free radical injury,NO synthesis and release,sequestration of neutrophilic granulocytes,decreasing activity of cell membrane pumps and energy metabolism dysfunction.Pathogenesis of the intestinal lymphatic pathway should be thoroughly investigated to prevent ischemia/reperfusion injury. | Zigang Zhao Chunyu Niu Aimin Shang Jiaming Tian Rui Han Chunhui Zhang Yuping Zhang Jing Zhang | 2010 | Neural Regeneration Research2010，5，9： | 7 |
| 2 | Influence of mesenteric lymph reperfusion on neurotransmitter expression in brain tissue of a superior mesenteric artery occlusion shock rat model显示文摘BACKGROUND: Previous studies have shown that mesenteric lymph reperfusion (MLR) exacerbates brain injury in a rat model of superior mesenteric artery occlusion (SMAO) shock. However, little is known about the influence of MLR on neurotransmitter expression in brain tissue. OBJECTIVE: To observe the effect of MLR on brain tissue injury by measuring monoamine and cholinergic neurotransmitter levels. DESIGN, TIME AND SETTING: A randomized, controlled, animal experiment was performed at the Institute of Microcirculation, Hebei North University, China; Research Room of Microcirculation and Laboratory of Biochemistry, Department of Pathophysiology, Basic Medical College, Hebei North University between December 2007 and March 2009. MATERIALS: Choline acetyltransferase (ChAT) and acetylcholine esterase (AChE) kits were provided by Nanjing Jiancheng Bioengineering Institute, China; dopamine (DA) and noradrenalin (NE) standards were provided by the National Institute for the Control of Pharmaceutical and Biological Products; HP1100 chromatograph of liquid was provided by Agilent, USA. METHODS: A total of 24 male, Wistar rats were randomly assigned to 4 groups: sham-surgery, MLR, SMAO, and MLR + SMAO groups, with 6 rats in each group. In the MLR or SMAO groups, the mesenteric lymph duct or superior mesenteric artery was blocked for 1 hour. In the MLR + SMAO group, the mesenteric lymph duct and superior mesenteric artery were occluded for 1 hour, followed by 2-hour reperfusion. ChAT and AChE levels were measured using the synthesized and hydrolyzed acetylcholine method, respectively. Liquid chromatography was employed to quantitatively analyze DA and NE levels, using relative retention time and the external standard method. MAIN OUTCOME MEASURES: ChAT, AChE, DA, and NE levels. RESULTS: AChE levels were significantly increased, but ChAT levels were significantly decreased in the MLR and MLR + SMAO groups following 2-hour reperfusion (P < 0.01). However, AChE activity in the MLR + SMAO group was greater than in the MLR group (P < 0.05). DA and NE levels were significantly decreased in the SMAO and MLR + SMAO groups (P < 0.01), while DA levels in the MLR + SMAO group were less than in the SMAO group (P < 0.05). CONCLUSION: MLR exacerbated brain injury in a rat model of SMAO shock, which correlated with the intestinal lymphatic pathway. MLR decreased DA levels, but increased AChE activity, in a rat model of SMAO shock. | Chunyu Niu Zigang Zhao Aimin Shang Jiaming Tian Chunhui Zhang Rui Han Yuping Zhang Jing Zhang | 2010 | Neural Regeneration Research2010，5，11： | 2 |
| 3 | Multimerization and fusion expression of bovine lactoferricin derivative LfcinB 15-W4, 10 in Escherichia coli显示文摘 | TIAN Zigang TENG Da YANG Yalin | 2007 | Appl Microbiol Biotechnol2007，75，1： | 1 |
| 4 | High-level production of a candidacidal peptide lactoferrampin in Escherichia coli by fusion expression显示文摘 | YANG Yalin TIAN Zigang TENG Da | 2009 | J Biotechnol2009，139，4： | 1 |
| 5 | Muhimerization and fusion ex- pression of bovine lactoferricin derivative LfcinBl5-W4, 10 in Escherichia coli 显示文摘 | Tian Zigang Teng Da Yang Yalin | 2007 | Appl Microbiol Biotechnol2007，，75： | 1 |
| 6 | Erianin suppresses constitutive activation of MAPK signaling pathway by inhibition of CRAF and MEK1/2显示文摘Constitutive activation of RAS-RAF-MEK-ERK signaling pathway(MAPK pathway)frequently occurs in many cancers harboring RAS or RAF oncogenic mutations.Because of the paradoxical activation induced by a single use of BRAF or MEK inhibitors,dual-target RAF and MEK treatment is thought to be a promising strategy.In this work,we evaluated erianin is a novel inhibitor of CRAF and MEK1/2 kinases,thus suppressing constitutive activation of the MAPK signaling pathway induced by BRAF V600E or RAS mutations.KinaseProfiler enzyme profiling,surface plasmon resonance(SPR),isothermal titration calorimetry(ITC),cellular thermal shift assay,computational docking,and molecular dynamics simulations were utilized to screen and identify erianin binding to CRAF and MEK1/2.Kinase assay,luminescent ADP detection assay,and enzyme kinetics assay were investigated to identify the efficiency of erianin in CRAF and MEK1/2 kinase activity.Notably,erianin suppressed BRAF V600E or RAS mutant melanoma and colorectal cancer cell by inhibiting MEK1/2 and CRAF but not BRAF kinase activity.Moreover,erianin attenuated melanoma and colorectal cancer in vivo.Overall,we provide a promising leading compound for BRAF V600E or RAS mutant melanoma and colorectal cancer through dual targeting of CRAF and MEK1/2. | Penglei Wang Xuechao Jia Bingbing Lu Han Huang Jialin Liu Xuejiao Liu Qiong Wu Yamei Hu Pan Li Huifang Wei Tingting Liu Dengyun Zhao Lingwei Zhang Xueli Tian Yanan Jiang Yan Qiao Wenna Nie Xinli Ma Ruihua Bai Cong Peng Zigang Dong Kangdong Liu | 2023 | Signal Transduction and Targeted Therapy2023，8，4： | 1 |
| 7 | Facial paralysis in cerebral infarction:A case of misdiagnosis and literature review显示文摘Facial paralysis can be classified as central or peripheral facial paralysis based on the location of the underlying lesion,both of which demonstrate facial motor dysfunction.In the currently report,a patient admitted to the department of otology,First People’s Hospital of Qinhuangdao,presented with facial asymmetry as the initial symptom of a cerebral infarction and was first misdiagnosed as peripheral facial paralysis.The case is reported as follows. | Xin Li Xiaoyan Zhang Xiaobin Tian Zigang Jiang Baohuan Li | 2014 | Journal of Otology2014，9，4： | 1 |
| 8 | RIN1 exhibits oncogenic property to suppress apoptosis and its aberrant accumulation associates with poor prognosis in melanoma显示文摘 | Ping Fang Zigang Zhao Hongfang Tian Xin Zhang | 2012 | Tumor Biology2012，，5： | 1 |
| 9 | A novel selective ERK1/2 inhibitor,Laxiflorin B,targets EGFR mutation subtypes in non-small-cell lung cancer显示文摘Extracellular regulated protein kinases 1/2(ERK1/2)are key members of multiple signaling pathways,including the ErbB axis.Ectopic ERK1/2 activation contributes to various types of cancer,especially drug resistance to inhibitors of RTK,RAF and MEK,and specific ERK1/2 inhibitors are scarce.In this study,we identified a potential novel covalent ERK inhibitor,Laxiflorin B,which is a herbal compound with anticancer activity.However,Laxiflorin B is present at low levels in herbs;therefore,we adopted a semi-synthetic process for the efficient production of Laxiflorin B to improve the yield.Laxiflorin B induced mitochondria-mediated apoptosis via BAD activation in non-small-cell lung cancer(NSCLC)cells,especially in EGFR mutant subtypes.Transcriptomic analysis suggested that Laxiflorin B inhibits amphiregulin(AREG)and epiregulin(EREG)expression through ERK inhibition,and suppressed the activation of their receptors,ErbBs,via a positive feedback loop.Moreover,mass spectrometry analysis combined with computer simulation revealed that Laxiflorin B binds covalently to Cys-183 in the ATP-binding pocket of ERK1 via the D-ring,and Cys-178 of ERK1 through non-inhibitory binding of the A-ring.In a NSCLC tumor xenograft model in nude mice,Laxiflorin B also exhibited strong tumor suppressive effects with low toxicity and AREG and EREG were identified as biomarkers of Laxiflorin B efficacy.Finally,Laxiflorin B-4,a C-6 analog of Laxiflorin B,exhibited higher binding affinity for ERK1/2 and stronger tumor suppression.These findings provide a new approach to tumor inhibition using natural anticancer compounds. | Cheng-Yao Chiang Min Zhang Junrong Huang Juan Zeng Chunlan Chen Dongmei Pan Heng Yang Tiantian Zhang Min Yang Qiangqiang Han Zou Wang Tian Xiao Yangchao Chen Yongdong Zou Feng Yin Zigang Li Lizhi Zhu Duo Zheng | 2024 | Acta Pharmacologica Sinica2024，45，2： | 0 |
| 10 | Development of a potent peptide inhibitor of estrogen receptor α显示文摘We have developed a facile N-terminus helix-nucleating strategy using an unnaturally tethered aspartic acid(TD strategy). Relatively weak nuclear translocation efficiency of TD PERM limits its further biological applications. A potent peptide inhibitor of estrogen receptor α(ER-α) with significantly increased cellular uptake and cellular distribution was developed by cell penetrating peptide attachment.The resulted peptide conjugate showed selective toxicity towards estrogen receptor positive cell lines and induced decreased transcription of estrogen receptor a downstream genes. | Xuan Qin Hui Zhao Yanhong Jiang Feng Yin Yuan Tian Mingsheng Xie Xiyang Ye Naihan Xu Zigang Li | 2018 | Chinese Chemical Letters2018，29，7： | 0 |
| 11 | Reversibly switching the conformation of short peptide through in-tether chiral sulfonium auxiliary显示文摘A chirality induced helicity method has been developed to modulate the peptide's biophysical and biochemical properties. We report herein a novel approach for reversibly switching the conformation of short constraint a-helical peptides through alkylation of the in-tether thioether and dealkylation of the chiral sulfonium. This traceless redox sensitive tagging strategy broadened our scope of CIH(chirality induced helicity) strategy and provided a valuable approach to functionalize the peptide tether. | Xiaodong Shi Yixiang Jiang Dan Yang Hui Zhao Yuan Tian Zigang Li | 2018 | Chinese Chemical Letters2018，29，3： | 0 |
| 12 | Recent advances in chemical protein synthesis:method developments and biological applications显示文摘The central dogma of modern biology underscores the pivotal roles proteins play in diverse biological processes,the study of which necessitates advanced methods to produce proteins with precision and versatility.Chemical protein synthesis,a powerful approach utilizing chemical reactions for the de novo construction of structurally accurate proteins,has emerged as a transformative tool for studying proteins and generating protein derivatives/mimics inaccessible by natural biological machinery,including post-translationally modified proteins,proteins comprised of unnatural amino acids,as well as mirror-image proteins.This review summarizes recent strides in synthetic method developments for chemical protein synthesis,including innovative techniques in solid-phase peptide synthesis,the challenges presented by difficult sequences in either synthesis or folding and the exploration of novel ligation reactions using both chemical and enzymatic methods.Furthermore,the review also delves into newly developed protocols for site-selective protein modifications and the generation of stapled or macrocyclized peptides/miniproteins,highlighting the power of chemical methods to make structurally diverse proteins.Recent applications of synthetic proteins in investigating post-translational modifications(phosphorylation,lipidation,glycosylation,ubiquitination,etc.),mirror-image biological processes and drug development are further discussed.Together,these topics provide a comprehensive overview of the current landscape of chemical protein synthesis. | Suwei Dong Ji-Shen Zheng Yiming Li Huan Wang Gong Chen Yongxiang Chen Gemin Fang Jun Guo Chunmao He Honggang Hu Xuechen Li Yanmei Li Zigang Li Man Pan Shan Tang Changlin Tian Ping Wang Bian Wu Chuanliu Wu Junfeng Zhao Lei Liu | 2024 | Science China Chemistry2024，67，4： | 0 |
| 13 | Acetylshikonin inhibits inflammatory responses and Papain-like protease activity in murine model of COVID-19显示文摘Dear Editor,The emerging severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)variant Omicron has rapidly replaced the Delta variant and presents a huge challenge to public health and health care infrastructure.Cytokine-driven hyperinflammation is the leading cause of a series of COVID-19 clinical symptoms.1 Our research attempted to discover an anti-inflammatory or anti-viral small molecule agent capable of treating COVID-19 hyperinflammation. | Ning Lu Tingxuan Gu Xueli Tian Simin Zhao Guoguo Jin Fredimoses Mangaladoss Yan Qiao Kangdong Liu Ran Zhao Zigang Dong | 2022 | Signal Transduction and Targeted Therapy2022，7，11： | 0 |
| 14 | Proximity-induced amino-yne reaction for selective MDM4 conjugation via propargylated sulfonium显示文摘Whilst most bioorthogonal reactions focus on targeting binding-site cysteine residues,proximity-induced reactivity effect ensures that reaction also occurs at nucleophilic lysine residues.We report one example here that the propargylated-sulfonium center undergoes a nucleophilic reaction with lysine residue via proximity-induced conjugation.This propargylated-sulfonium tethered peptide resulting from a facile propargylation of thiolethers,enables amino-yne reaction at the selected lysine on MDM4 protein.This strategy represents a viable approach of lysine-targeted covalent inhibition in proximity. | Chenshan Lian Yang Li Zhanfeng Hou Wanjin Zhong Ye Tian Feng Yin Zigang Li Dongxian Zhou Rui Wang | 2021 | Chinese Chemical Letters2021，32，11： | 0 |
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