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| 1 | A novel coronavirus (2019-nCoV) causing pneumonia-associated respiratory syndrome显示文摘By the end of 2019,the World Health Organization(WHO)was informed about an outbreak of pneumonia of unknown etiology in Wuhan,China.Some patients were linked to a seafood market,suggesting animal-to-human transmission,but,soon,human-tohuman transmission was confirmed.The pathogen was promptly identified as a novel coronavirus belonging to lineage B betacoronaviruses that also include severe acute respiratory syndrome coronavirus(SARS-CoV),which caused a pandemic in China in 2002/2003,and bat SARS-like coronaviruses. | Shibo Jiang Shuai Xia Tianlei Ying Lu Lu | 2020 | Cellular & Molecular Immunology2020，17，5： | 33 |
| 2 | Fusion mechanism of 2019-nCoV and fusion inhibitors targeting HR1 domain in spike protein显示文摘Very recently,a novel coronavirus,2019-nCoV,emerged in Wuhan,China and then quickly spread worldwide,resulting in>17,388 confirmed cases and 361 deaths as of 3 February 2020,thus calling for the development of safe and effective therapeutics and prophylatics.1,2 Similar to severe acute respiratory syndrome(SARS)-CoV,2019-nCoV belongs to lineage B betacoronavirus,and it has the ability to utilize human angiotensin-converting enzyme 2(ACE2)as a receptor to infect human cells. | Shuai Xia Yun Zhu Meiqin Liu Qiaoshuai Lan Wei Xu Yanling Wu Tianlei Ying Shuwen Liu Zhengli Shi Shibo Jiang Lu Lu | 2020 | Cellular & Molecular Immunology2020，17，7： | 27 |
| 3 | Human monoclonal antibodies as candidate therapeutics against emerging viruses显示文摘 | Yujia Jin Cheng Lei Dan Hu Dimiter S. Dimitrov Tianlei Ying | 2017 | Frontiers of Medicine2017，11，4： | 8 |
| 4 | RBD-Fc-based COVID-19 vaccine candidate induces highly potent SARS-CoV-2 neutralizing antibody response显示文摘The pandemic of coronavirus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has posed serious threats to global health and economy,thus calling for the development of safe and effective vaccines.The receptorbinding domain(RBD)in the spike protein of SARS-CoV-2 is responsible for its binding to angiotensin-converting enzyme 2(ACE2)receptor.It contains multiple dominant neutralizing epitopes and serves as an important antigen for the development of COVID-19 vaccines.Here,we showed that immunization of mice with a candidate subunit vaccine consisting of SARS-CoV-2 RBD and Fc fragment of human IgG,as an immunopotentiator,elicited high titer of RBD-specific antibodies with robust neutralizing activity against both pseudotyped and live SARS-CoV-2 infections.The mouse antisera could also effectively neutralize infection by pseudotyped SARS-CoV-2 with several natural mutations in RBD and the IgG extracted from the mouse antisera could also show neutralization against pseudotyped SARS-CoV and SARS-related coronavirus(SARSr-CoV).Vaccination of human ACE2 transgenic mice with RBD-Fc could effectively protect mice from the SARS-CoV-2 challenge.These results suggest that SARS-CoV-2 RBD-Fc has good potential to be further developed as an effective and broad-spectrum vaccine to prevent infection of the current SARS-CoV-2 and its mutants,as well as future emerging SARSr-CoVs and re-emerging SARS-CoV. | Zezhong Liu Wei Xu Shuai Xia Chenjian Gu Xinling Wang Qian Wang Jie Zhou Yanling Wu Xia Cai Di Qu Tianlei Ying Youhua Xie Lu Lu Zhenghong Yuan Shibo Jiang | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 7 |
| 5 | Parallel ADR Detection Based on Spark and BCPNN显示文摘Adverse Drug Reaction(ADR) is one of the major challenges to the evaluation of drug safety in the medical field. The Bayesian Confidence Propagation Neural Network(BCPNN) algorithm is the main algorithm used by the World Health Organization to monitor ADRs. Currently, ADR reports are collected through the spontaneous reporting system. However, with the continuous increase in ADR reports and possible use scenarios, the efficiency of the stand-alone ADR detection algorithm will encounter considerable challenges. Meanwhile, the BCPNN algorithm requires a certain number of disk I/O, which leads to considerable time consumption. In this study,we propose a Spark-based parallel BCPNN algorithm, which speeds up data processing and reduces the number of disk I/O in BCPNN, and two optimization strategies. Then, the ADR data collected from the FDA Adverse Event Reporting System are used to verify the performance of the proposed algorithm and its optimization strategies.Experiments show that the parallel BCPNN can significantly accelerate data processing and the optimized algorithm has a high acceleration rate and can effectively prevent memory overflow. Finally, we apply the proposed algorithm to a dataset provided by a real medical consortium. Experiments further prove the performance and practical value of the proposed algorithm. | Li Sun Shan Sun Tianlei Wang Jiyun Li Jingsheng Lin | 2019 | Tsinghua Science and Technology2019，24，2： | 4 |
| 6 | Recent advances in developing small-molecule inhibitors against SARS-CoV-2显示文摘The COVID-19 pandemic caused by the novel SARS-CoV-2 virus has caused havoc across the entire world.Even though several COVID-19 vaccines are currently in distribution worldwide,with others in the pipeline,treatment modalities lag behind.Accordingly,researchers have been working hard to understand the nature of the virus,its mutant strains,and the pathogenesis of the disease in order to uncover possible drug targets and effective therapeutic agents.As the research continues,we now know the genome structure,epidemiological and clinical features,and pathogenic mechanism of SARS-CoV-2.Here,we summarized the potential therapeutic targets involved in the life cycle of the virus.On the basis of these targets,small-molecule prophylactic and therapeutic agents have been or are being developed for prevention and treatment of SARS-CoV-2 infection. | Rong Xiang Zhengsen Yu Yang Wang Lili Wang Shanshan Huo Yanbai Li Ruiying Liang Qinghong Hao Tianlei Ying Yaning Gao Fei Yu Shibo Jiang | 2022 | Acta Pharmaceutica Sinica B2022，12，4： | 3 |
| 7 | Receptor-binding domain-specific human neutralizing monoclonal antibodies against SARS-CoV and SARS-CoV-2显示文摘The outbreaks of severe acute respiratory syndrome(SARS)and Coronavirus Disease 2019(COVID-19)caused by SARS-CoV and SARS-CoV-2,respectively,have posed severe threats to global public health and the economy.Treatment and prevention of these viral diseases call for the research and development of human neutralizing monoclonal antibodies(NMAbs).Scientists have screened neutralizing antibodies using the virus receptor-binding domain(RBD)as an antigen,indicating that RBD contains multiple conformational neutralizing epitopes,which are the main structural domains for inducing neutralizing antibodies and T-cell immune responses.This review summarizes the structure and function of RBD and RBD-specific NMAbs against SARS-CoV and SARS-CoV-2 currently under development. | Fei Yu Rong Xiang Xiaoqian Deng Lili Wang Zhengsen Yu Shijun Tian Ruiying Liang Yanbai Li Tianlei Ying Shibo Jiang | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 3 |
| 8 | Antibody Cocktail Exhibits Broad Neutralization Activity Against SARS-CoV-2 and SARS-CoV-2 Variants显示文摘Severe acute respiratory syndrome coronavirus 2(SARS-Co V-2)has precipitated multiple variants resistant to therapeutic antibodies.In this study,12 high-affinity antibodies were generated from convalescent donors in early outbreaks using immune antibody phage display libraries.Of them,two RBD-binding antibodies(F61 and H121)showed high-affinity neutralization against SARS-Co V-2,whereas three S2-target antibodies failed to neutralize SARS-Co V-2.Following structure analysis,F61 identified a linear epitope located in residues G446–S494,which overlapped with angiotensinconverting enzyme 2(ACE2)binding sites,while H121 recognized a conformational epitope located on the side face of RBD,outside from ACE2 binding domain.Hence the cocktail of the two antibodies achieved better performance of neutralization to SARS-Co V-2.Importantly,these two antibodies also showed efficient neutralizing activities to the variants including B.1.1.7 and B.1.351,and reacted with mutations of N501 Y,E484 K,and L452 R,indicated that it may also neutralize the recent India endemic strain B.1.617.The unchanged binding activity of F61 and H121 to RBD with multiple mutations revealed a broad neutralizing activity against variants,which mitigated the risk of viral escape.Our findings revealed the therapeutic basis of cocktail antibodies against constantly emerging SARS-Co V-2 variants and provided promising candidate antibodies to clinical treatment of COVID-19 patients infected with broad SARS-Co V-2 variants. | Yuanyuan Qu Xueyan Zhang Meiyu Wang Lina Sun Yongzhong Jiang Cheng Li Wei Wu Zhen Chen Qiangling Yin Xiaolin Jiang Yang Liu Chuan Li Jiandong Li Tianlei Ying Dexin Li Faxian Zhan Youchun Wang Wuxiang Guan Shiwen Wang Mifang Liang | 2021 | Virologica Sinica2021，36，5： | 3 |
| 9 | Bimetallic PtSn nanoparticles confined in hierarchical ZSM-5 for propane dehydrogenation显示文摘A series of PtSn/hierarchical ZSM-5 catalysts were developed for propane dehydrogenation,in which the PtSn bimetallic particles are confined in the mesopores of hierarchical ZSM-5 zeolite.The synthesis of PtSn/hierarchical ZSM-5 catalysts was achieved via the loading of Pt and Sn species onto the hierarchical ZSM-5 catalysts that are obtained through a desilication of conventional ZSM-5.The PtSn/hierarchical ZSM-5 catalysts were fully characterized by XRD,N_(2) adsorption,STEM,XPS,and CO-IR techniques,which reveals that highly dispersed PtSn bimetallic nanoparticles are enclosed into mesopores of hierarchical ZSM-5.The catalytic performance of PtSn/hierarchical ZSM-5 is greatly affected by the concentrations of alkali solution in the desilication process and Sn/Pt ratios in PtSn bimetallic particles.The PtSn1.00/ZSM-5(0.8)catalyst shows the highest efficiency in propane dehydrogenation,which gives an initial conversion of 46%and selectivity of 98%at 570℃.The high efficiency in these PtSn/hierarchical ZSM-5 catalysts for propane dehydrogenation is mainly ascribed to the confinement of PtSn particles in the mesopores of hierarchical ZSM-5 zeolite. | Tianlei Wang Zhikang Xu Yuanyuan Yue Tinghai Wang Minggui Lin Haibo Zhu | 2022 | Chinese Journal of Chemical Engineering2022，35，1： | 2 |
| 10 | Syntheses and Anti-cancer Activities of Derivatives of Tetrandrine and Fangchinoline显示文摘 | LIU Yazhou HUANG Lan SUN Qianyun ZHANG Maosheng LI Tianlei LIANG Guangyi PAN Weidong | 2014 | Chemical Research in Chinese Universities2014，30，6： | 2 |
| 11 | Structure modeling and spatial epitope analysis for HA protein of the novel H1N1 influenza virus显示文摘In recent months,a novel influenza virus H1N1 broke out around the world.With bioinformatics technology,the 3D structure of HA protein was obtained,and the epitope residues were predicted with the method developed in our group for this novel flu virus.58 amino acids were identified as potential epitope residues,the majority of which clustered at the surface of the globular head of HA protein.Although it is located at the similar position,the epitope of HA protein for the novel H1N1 flu virus has obvious differences in the electrostatic potential compared to that of HA proteins from previous flu viruses. | WU Di XU TianLei SUN Jing DAI JianXin DING GuoHui HE YunGang ZHOU ZhengFeng XIONG Hui DONG Hui JIN WeiRong BIAN Chao JIN Li WANG HongYan WANG XiaoNing YANG Zhong ZHONG Yang WANG Hao CHE XiaoYan HUANG Zhong LAN Ke SUN Bing WU Fan YUAN ZhenAn ZHANG Xi ZHOU XiaoNong ZHOU JiaHai MA ZhiYong TONG GuangZhi GUO YaJun ZHAO GuoPing LI YiXue CAO ZhiWei | 2009 | Chinese Science Bulletin2009，54，13： | 2 |
| 12 | Evaluation of spatial epitope computational tools based on experimentally-confirmed dataset for protein antigens显示文摘Antibody molecules interact with antigen proteins through the epitope area,where the epitope residues are found to be discontinuous or spatial or conformational rather than linear on the protein surface.There are various computational algorithms to predict the spatial epitopes,and each of them have an outstanding performance based on their individual testing dataset.In this work,an independent dataset was created through collection of the epitope residual sites which have been confirmed by experiments. Based on this dataset,6 popular web-servers developed for B-cell structural epitope prediction,including SEPPA,CEP,DiscoTope,ElliPro,PEPOP and BEpro,were evaluated and compared according to sensitivity,the positive predictive value,the successful pick-up rate and the area under the curve of the receiver operator characteristic(AUC).The results showed that the general performance of spatial epitope prediction tools did obtain substantial advancement,and SEPPA gave the best performance among the 6 tools.However,the current prediction accuracy was still far from satisfaction.Moreover,our comparison elucidated that the performance of the web-servers was significantly affected by their training datasets and the algorithms adopted.In this sense,the results of our research may improve the design of B-cell epitope prediction tools and provide additional clues when the users utilize these tools in their related research. | XU XiaoLian SUN Jing LIU Qi WANG XiaoJing XU TianLei ZHU RuiXin WU Di CAO ZhiWei | 2010 | Chinese Science Bulletin2010，55，20： | 2 |
| 13 | Stability and Hopf bifurcation for a virus infection model with delayed humoral immunity response显示文摘 | Tianlei Wang Zhixing Hu Fucheng Liao | 2014 | J Math Anal Appl2014，411，： | 1 |
| 14 | Total dose radiation hardened PDSOI CMOS 64 K SRAMS显示文摘 | GUO Tianlei ZHAO Fazhan LIU Gang | 2007 | Chinese J Semicond2007，28，11： | 1 |
| 15 | Numerical simulation on the mixing behavior of pin unit under vibrant enhancement显示文摘 | Liu Tianlei Du Yaoxue | 2011 | Polymer-Plastics Technology and Engineering2011，50，12： | 1 |
| 16 | Traffic light detection and recognition for autonomous vehicles显示文摘Traffic light detection and recognition is essential for autonomous driving in urban environments. A camera based algorithm for real-time robust traffic light detection and recognition was proposed, and especially designed for autonomous vehicles. Although the current reliable traffic light recognition algorithms operate well under way, most of them are mainly designed for detection at a fixed position and the effect on autonomous vehicles under real-world conditions is still limited. Some methods achieve high accuracy on autonomous vehicle, but they can't work normally without the aid of high-precision priori map. The authors presented a camera-based algorithm for the problem. The image processing flow can be divided into three steps, including pre-processing, detection and recognition. Firstly, red-green-blue(RGB) color space is converted to hue-saturation-value(HSV) as main content of pre-processing. In detection step, the transcendental color threshold method is used for initial filterings, meanwhile, the prior knowledge is performed to scan the scene in order to quickly establish candidate regions. For recognition, this article use histogram of oriented gradients(HOG) features and support vector machine(SVM) as well to recognize the state of traffic light. The proposed system on our autonomous vehicle was evaluated. With voting schemes, the proposed can provide a sufficient accuracy for autonomous vehicles in urban enviroment. | Guo Mu Zhang Xinyu Li Deyi Zhang Tianlei An Lifeng | 2015 | The Journal of China Universities of Posts and Telecommunications2015，22，1： | 1 |
| 17 | A non-ACE2 competing human single-domain antibody confers broad neutralization against SARS-CoV-2 and circulating variants显示文摘The current COVID-19 pandemic has heavily burdened the global public health system and may keep simmering for years.The frequent emergence of immune escape variants have spurred the search for prophylactic vaccines and therapeutic antibodies that confer broad protection against SARS-CoV-2 variants.Here we show that the bivalency of an affinity maturated fully human singledomain antibody(n3113.1-Fc)exhibits exquisite neutralizing potency against SARS-CoV-2 pseudovirus,and confers effective prophylactic and therapeutic protection against authentic SARS-CoV-2 in the host cell receptor angiotensin-converting enzyme 2(ACE2)humanized mice.The crystal structure of n3113 in complex with the receptor-binding domain(RBD)of SARS-CoV-2,combined with the cryo-EM structures of n3113 and spike ecto-domain,reveals that n3113 binds to the side surface of up-state RBD with no competition with ACE2.The binding of n3113 to this novel epitope stabilizes spike in up-state conformations but inhibits SARS-CoV-2 S mediated membrane fusion,expanding our recognition of neutralization by antibodies against SARS-CoV-2.Binding assay and pseudovirus neutralization assay show no evasion of recently prevalent SARS-CoV-2 lineages,including Alpha(B.1.1.7),Beta(B.1.351),Gamma(P.1),and Delta(B.1.617.2)for n3113.1-Fc with Y58L mutation,demonstrating the potential of n3113.1-Fc(Y58L)as a promising candidate for clinical development to treat COVID-19. | Zhenlin Yang Yulu Wang Yujia Jin Yuanfei Zhu Yanling Wu Cheng Li Yu Kong Wenping Song Xiaolong Tian Wuqiang Zhan Ailing Huang Shanshan Zhou Shuai Xia Xiaoxu Tian Chao Peng Cuicui Chen Yibing Shi Gaowei Hu Shujuan Du Yuyan Wang Youhua Xie Shibo Jiang Lu Lu Lei Sun Yuanlin Song Tianlei Ying | 2021 | Signal Transduction and Targeted Therapy2021，6，12： | 1 |
| 18 | Recent advances in“universal”influenza virus antibodies:the rise of a hidden trimeric interface in hemagglutinin globular head显示文摘Influenza causes seasonal outbreaks yearly and unpredictable pandemics with high morbidity and mortality rates.Despite significant efforts to address influenza,it remains a major threat to human public health.This issue is partially due to the lack of antiviral drugs with potent antiviral activity and broad reactivity against all influenza virus strains and the rapid emergence of drug-resistant variants.Moreover,designing a universal influenza vaccine that is sufficiently immunogenic to induce universal antibodies is difficult.Some novel epitopes hidden in the hemagglutinin(HA)trimeric interface have been discovered recently,and a number of antibodies targeting these epitopes have been found to be capable of neutralizing a broad range of influenza isolates.These findings may have important implications for the development of universal influenza vaccines and antiviral drugs.In this review,we focused on the antibodies targeting these newly discovered epitopes in the HA domain of the influenza virus to promote the development of universal anti-influenza antibodies or vaccines and extend the discovery to other viruses with similar conformational changes in envelope proteins. | Yulu Wang Dan Hu Yanling Wu Tianlei Ying | 2020 | Frontiers of Medicine2020，14，2： | 1 |
| 19 | Analysis and Design of the Control Strategy of Three-roll Rewinder显示文摘By dividing the rewinding process of a three-roll rewinder into three zones, mathematical equations were formulated and diagrams were drawn to determine the structure factors of the rewinder, such as the diameters and spatial distribution of the rewinding rolls. The qualitative and quantitative analysis results show that the roll gap of the first two rewinding rolls and the angle between the line that connects the center points of the first two rewinding rolls and the vertical line play an important role in determining the ideal log diameter, which is related to the production capacity of the rewinder. Additionally, the ideal roll gap has a significant effect on the spatial distribution of the three rewinding rolls, the rewinding dead zone, and the rewinding quality of the log, which should be taken into full consideration when designing and improving rewinders to enhance production efficiency and satisfy ultra-high quality requirements. | TianLei Liu | 2017 | Paper And Biomaterials2017，2，2： | 1 |
| 20 | Establishment of Novel Monoclonal Fabs Specific for Epstein-Barr Virus Encoded Latent Membrane Protein 1显示文摘Dear Editor,Epstein-Barr virus(EBV,also termed human herpesvirus-4)was the first identified human tumor virus.Since its discovery in 1964,studies have shown that EBV infects over 90%of all people by the time they are adults(Williams and Crawford 2006).EBV infection can result in mucocutaneous and systemic diseases,ranging from selflimited illnesses to aggressive malignancies,including B cell Hodgkin lymphoma and nasopharyngeal carcinoma.In vitro,EBV transforms resting B cells into proliferating blast cells(Pope et al.1968). | Gaoxin Li Ling Ding Xiaojing Ma Qiliang Cai Tianlei Ying Fang Wei | 2019 | Virologica Sinica2019，34，4： | 1 |
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