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| 1 | Enhancing Heterogeneous Catalytic Activity of Iron(II) Phthalocyanine by Ethanol and Its Application in 2,4-dichlorophenol Detection显示文摘A chemical system for facile and accurate detection of 2,4-dichlorophenol(DCP) via iron(II) phthalocyanine(Fe(II)Pc) catalyzed chromogenic reaction is reported for the fi rst time. In this system, DCP could be oxidized by dioxygen with the catalysis of Fe(II)Pc and then coupled with 4-aminoantipyrine(4-AAP) to generate pink antipyrilquinoneimine dye. Control experiments showed that the addition of ethanol could obviously enhance the catalytic activity of heterogeneous Fe(II)Pc catalysts because of the partial dissolution of Fe(II)Pc nanocubes, which was confirmed by the SEM analysis. On the basis of the detection results of DCP in the range from 2×10-5 to 9×10-4 mol/L, we obtained a regression equation(A = 0.187 5 + 0.01 209C(R2=0.995 6)) with the detection limit(3σ) of 3.26×10-6 mol/L, which could be successfully used in detecting the real samples. | 童伊琳 LI Dapeng HUANG Jun LI Kun DING Liyun WANG Tianxia GONG Jingjing | 2014 | Journal of Wuhan University of Technology(Materials Science)2014，29，3： | 1 |
| 2 | Crosstalk between CYP2E1 and PPARα substrates and agonists modulate adipose browning and obesity显示文摘Although the functions of metabolic enzymes and nuclear receptors in controlling physiological homeostasis have been established, their crosstalk in modulating metabolic disease has not been explored.Genetic ablation of the xenobiotic-metabolizing cytochrome P450 enzyme CYP2 E1 in mice markedly induced adipose browning and increased energy expenditure to improve obesity. CYP2 E1 deficiency activated the expression of hepatic peroxisome proliferator-activated receptor alpha(PPARa) target genes,including fibroblast growth factor(FGF) 21, that upon release from the liver, enhanced adipose browning and energy expenditure to decrease obesity. Nineteen metabolites were increased in Cyp2 e1-null mice as revealed by global untargeted metabolomics, among which four compounds, lysophosphatidylcholine and three polyunsaturated fatty acids were found to be directly metabolized by CYP2 E1 and to serve as PPARa agonists, thus explaining how CYP2 E1 deficiency causes hepatic PPARa activation through increasing cellular levels of endogenous PPARa agonists. Translationally, a CYP2 E1 inhibitor was found to activate the PPARa-FGF21-beige adipose axis and decrease obesity in wild-type mice, but not in liver-specific Pparanull mice. The present results establish a metabolic crosstalk between PPARa and CYP2 E1 that supports the potential for a novel anti-obesity strategy of activating adipose tissue browning by targeting the CYP2 E1 to modulate endogenous metabolites beyond its canonical role in xenobiotic-metabolism. | Youbo Zhang Tingting Yan Tianxia Wang Xiaoyan Liu Keisuke Hamada Dongxue Sun Yizheng Sun Yanfang Yang Jing Wang Shogo Takahashi Qiong Wang Kristopher W.Krausz Changtao Jiang Cen Xie Xiuwei Yang Frank J.Gonzalez | 2022 | Acta Pharmaceutica Sinica B2022，12，5： | 1 |
| 3 | Intranasal administration of a recombinant RBD vaccine induces long-term immunity against Omicron-included SARS-CoV-2 variants显示文摘The outbreak of coronavirus disease 2019(COVID-19)has posed great threats to global health and economy.Several effective vaccines are available now,but additional booster immunization is required to retain or increase the immune responses owing to waning immunity and the emergency of new variant strains.The deficiency of intramuscularly delivered vaccines to induce mucosal immunity urged the development of mucosal vaccines.Here,we developed an adjuvanted intranasal RBD vaccine and monitored its long-term immunogenicity against both wild-type and mutant strains of severe acute respiratory syndrome coronavirus-2(SARSCoV-2),including Omicron variants,in mice.Three-dose intranasal immunization with this vaccine induced and maintained high levels of neutralizing IgG antibodies in the sera for at least 1 year.Strong mucosal immunity was also provoked,including mucosal secretory IgA and lung-resident memory T cells(TRM).We also demonstrated that the long-term persistence of lung TRM cells is a consequence of local T-cell proliferation,rather than T-cell migration from lymph nodes.Our data suggested that the adjuvanted intranasal RBD vaccine is a promising vaccine candidate to establish robust,long-lasting,and broad protective immunity against SARS-CoV-2 both systemically and locally. | Hong Lei Aqu Alu Jingyun Yang Wenyan Ren Cai He Tianxia Lan Xuemei He Li Yang Jiong Li Zhenling Wang Xiangrong Song Wei Wang Guangwen Lu Xiawei Wei | 2022 | Signal Transduction and Targeted Therapy2022，7，6： | 1 |
| 4 | Omicron variant:a booster depending on infection histories显示文摘Recently,Reynold et al.published a study in Science1 showing that immune imprinting patterns might differentially influence immune responses against variants of concern(VOCs)of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).They also demonstrated that infection with B.1.1.529(Omicron variant)of SARS-CoV-2 confers only partial and transient protection against itself,resulting in frequent reinfections with Omicron in short time intervals. | Jiayu Wang Tianxia Lan Yuquan Wei Yoshimasa Tanaka | 2023 | Signal Transduction and Targeted Therapy2023，8，2： | 0 |
| 5 | Formation of thermogenic adipocytes: What we have learned from pigs显示文摘Thermogenic adipocytes, including brown and beige adipocytes, are capable of dissipating energy via heat production in response to external stimuli, such as cold exposure and exercise. This distinct feature makes them promising targets for therapy of obesity and related metabolic disease. In most mammals, the thermogenic function relies on uncoupling protein 1 (UCP1), which creates a proton leak across the inner mitochondrial membrane, diverting protons away from ATP synthesis and resulting in heat dissipation. However, that is not the case in pigs. Accumulating evidence suggested that in pigs neither functional UCP1 nor brown adipocytes were identified, which might be the leading cause for cold stress-caused neonatal mortality and greater adiposity. In our previous study, beige adipocyte formation was found in cold-tolerant pig breeds, together with the finding that UCP1 knock-in improved thermoregulatory ability and decreased fat deposition in pigs, suggesting the potential application of beige induction in pigs for both agricultural purpose and biomedical implication. In this context, we review the anatomical features, developmental origins, plasticity, transcriptional regulation, and the metabolic benefits of thermogenic adipocytes. Particularly, we describe the contribution of beige adipocytes and reconstitution of UCP1 to improve cold sensitivity and decrease fat deposition in pigs. Furthermore, future undertaking regarding beige adipocyte formation in pigs and its potential application is also discussed. | Jianguo Zhao Cong Tao Chuanhe Chen Yanfang Wang Tianxia Liu | 2021 | Fundamental Research2021，1，4： | 0 |
| 6 | Boosting with variant-matched vaccines: an opportunity to win the race against Omicron显示文摘Recently,Baoling Ying et al.published a study in Cell that investigated whether using BA.1-matched or historical mRNA vaccine as a booster could enhance the protection against Omicron infection.1 In this study,the team evaluated the anti-Omicron efficacies of these two types of vaccines as primary(two-dose)immunization series or thirddose boosters in mice,respectively(Fig.1).Many of the current outbreaks of COVID-19 around the world have been shown to be driven by the Omicron variant of SARSCoV-2.2 Although vaccination remains one of the most efficient approaches to containing the pandemic,amino acid substitutions,deletions,and insertions in the spike protein of the Omicron variant have substantially impaired the efficacy of most licensed vaccines.3 Hence,more effective vaccines or vaccination strategies against Omicron are urgently needed. | Jiayu Wang Tianxia Lan Qiu Sun | 2022 | Signal Transduction and Targeted Therapy2022，7，7： | 0 |
| 7 | Antioxidant metabolic system and comparative proteomics analysis in winter turnip rape(Brassica rapa L.)under cold stress显示文摘Winter turnip rape(Brassica rapa L.)is widely cultivated in winter in Northwest China,however,its cold-tolerant mechanism remains insufficiently understood.In this study,winter turnip rape cultivar Longyou 7,a cold-tolerant variety,was used as material,whose accumulation of H_(2)O_(2) and O_(2)^(·-),antioxidant enzyme activity as well as differences in protein expression based on two-dimensional gel electrophoresis(2-DE)technique under -4℃ stress were analyzed.Results showed that,production of H_(2)O_(2) and O_(2)^(·-) were increased in Longyou 7 leaves,simultaneously,SOD and POD activities were also obviously rosed up,but the activities of CAT and APX were gradually reduced with the temperature.Thirty-six differential protein spots were successfully identified between control and treatments group by using mass spectrometry analysis.Among them,4 differential protein spots were induced under cold stress,and 2 were inhibited at-4℃.Functional analysis found that these identified proteins mainly participated in photosynthesis,carbohydrate metabolism,defense,protein synthesis,enzyme activity,redox and membrane metabolism,respectively.Additionally,13 proteins'function were still unknown.In conclusion,strong antioxidant capacity and cell defense ability might play important roles in Longyou 7 response to cold stress. | Xiucun Zeng Li Ma Jinhai Yuan Yaping Xie Tianxia Guan Xiaoqin Wang Guotai Ma Yaozhao Xu Wancang Sun | 2022 | Oil Crop Science2022，7，2： | 0 |
| 8 | Regulation of adenosine triphosphate-sensitive potassium channels suppresses the toxic effects of amyloid-beta peptide(25-35)显示文摘In this study, we treated PC12 cells with 0-20 μM amyloid-β peptide (25-35) for 24 hours to induce cytotoxicity, and found that 5-20 μM amyloid-β peptide (25-35) decreased PC12 cell viability, but adenosine triphosphate-sensitive potassium channel activator diazoxide suppressed the decrease in PC12 cell viability induced by amyloid-β peptide (25-35). Diazoxide protected PC12 cells against amyloid-β peptide (25-35)-induced increases in mitochondrial membrane potential and intracellular reactive oxygen species levels. These protective effects were reversed by the selective mitochondrial adenosine triphosphate-sensitive potassium channel blocker 5-hydroxydecanoate. An inducible nitric oxide synthase inhibitor, Nω-nitro-L-arginine, also protected PC12 cells from amyloid-β peptide (25-35)-induced increases in both mitochondrial membrane potential and intracellular reactive oxygen species levels. However, the H2O2-degrading enzyme catalase could not reverse the amyloid-β peptide (25-35)-induced increase in intracellular reactive oxygen species. A 24-hour exposure to amyloid-β peptide (25-35) did not result in apoptosis or necrosis, suggesting that the increases in both mitochondrial membrane potential and reactive oxygen species levels preceded cell death. The data suggest that amyloid-β peptide (25-35) cytotoxicity is associated with adenosine triphosphate-sensitive potassium channels and nitric oxide. Regulation of adenosine triphosphate-sensitive potassium channels suppresses PC12 cell cytotoxicity induced by amyloid-β peptide (25-35). | Min Kong Maowen Ba Hui Liang Peng Shao Tianxia Yu Ying Wang | 2013 | Neural Regeneration Research2013，8，1： | 0 |
| 9 | Constrained surfaces: promising therapeutic targets for COVID-19 determined by systematically mutational analysis显示文摘A recent study published in Cell by Tyler N.Starr et al. presented a quantitative deep mutational scanning platform that allowed for the characterization of potential amino acid(AA)mutations to the SARS-CoV-2 receptor binding domain(RBD).The mutationphenotype maps provided in this work determined the influence of RBD expression and binding affinity to human cell-surface protein angiotensin-converting enzyme 2(ACE2)induced by fully random substitution for each AA of RBD.This work identified pivotal positions for virus binding and entry into cells,facilitating the development of countermeasures for COVID-19,such as vaccines. | Manni Wang Tianxia Lan Wei Wang | 2021 | Signal Transduction and Targeted Therapy2021，6，2： | 0 |
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