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| 1 | ZmMs30 Encoding a Novel GDSL Lipase Is Essential for Male Fertility and Valuable for Hybrid Breeding in Maize显示文摘Genic male sterility (GMS) is very useful for hybrid vigor utilization and hybrid seed production. Although a large number of GMS genes have been identified in plants, little is known about the roles of GDSL lipase members in anther and pollen development. Here, we report a maize GMS gene, ZmMs30, which encodes a novel type of GDSL lipase with diverged catalytic residues. Enzyme kinetics and activity assays show that ZmMs30 has lipase activity and prefers to substrates with a short carbon chain. ZmMs30 is specifically expressed in maize anthers during stages 7-9. Loss of ZmMs30 function resulted in defective anther cuticle, irregular foot layer of pollen exine, and complete male sterility. Cytological and lipidomics analyses demonstrate that ZmMs30 is crucial for the aliphatic metabolic pathway required for pollen exine formation and anther cuticle development. Furthermore, we found that male sterility caused by loss of ZmMs30 function was stable in various inbred lines with different genetic background, and that it didn't show any negative effect on maize heterosis and production, suggesting that ZmMs30 is valuable for crossbreeding and hybrid seed production. We then developed a new multi-control sterility system using ZmMs30 and its mutant line, and demonstrated it is feasible for generating desirable GMS lines and valu. able for hybrid maize seed production. Taken together, our study sheds new light on the mechanisms of anther and pollen development, and provides a valuable male-sterility system for hybrid breeding maize. | Xueli An Zhenying Dong Youhui Tian Ke Xie Suowei Wu Taotao Zhu Danfeng Zhang Van Zhou Canfang Niu Biao Ma Quancan Hou Jianxi Bao Simiao Zhang Ziwen Li Yanbo Wang Tingwei Yan Xiaojing Sun Yuwen Zhang Jinping Li Xiangyuan Wan | 2019 | Molecular Plant2019，12，3： | 15 |
| 2 | 25-羟维生素D和甲状旁腺激素与高血压的相关性显示文摘有研究证实,维生素D缺乏和甲状旁腺激素(PTH)过高与高血压的发生密切相关。维生素D缺乏不仅可以影响肾素血管紧张素系统及PTH水平,而且可以直接参与血管炎性反应,影响高血压的发生发展。PTH可以通过促进血管炎性反应及血管钙化等参与高血压的进展。目前还没有前瞻性研究同时评估维生素D和PTH水平与高血压发病的关系。该研究分析多民族动脉粥样硬化研究的前瞻性数据,发现低水平25羟维生素D[25(OH)D]和高水平PTH与高血压发病相关,特别是在肥胖人群中。但在校正混杂因素后,25(OH)D水平与高血压发病的相关性无统计学意义,而PTH水平与高血压发病仍然相关。鉴于维生素D缺乏和PTH过量在人群中是普遍现象,该研究对于更好地了解高血压发病机制,在人群中开展高血压预防工作有重要意义。 | van Ballegooijen AJ Kestenbaum B Sachs MC de Boer IH Siscovick DS Hoofnagle AN Ix JH Visser M Brouwer IA 练桂丽 叶鹏 | 2014 | 中华高血压杂志2014，22，6： | 10 |
| 3 | Thermal-responsive genetic and epigenetic regulation of DAM cluster controlling dormancy and chilling requirement in peach floral buds显示文摘The Dormancy-associated MADS-box(DAM)gene cluster in peach serves as a key regulatory hub on which the seasonal temperatures act and orchestrate dormancy onset and exit,chilling response and floral bud developmental pace.Yet,how different temperature regimes interact with and regulate the six linked DAM genes remains unclear.Here,we demonstrate that chilling downregulates DAM1 and DAM3–6 in dormant floral buds with distinct patterns and identify DAM4 as the most abundantly expressed one.We reveal multiple epigenetic events,with tri-methyl histone H3 lysine 27(H3K27me3)induced by chilling specifically in DAM1 and DAM5,a 21-nt sRNA in DAM3 and a ncRNA induced in DAM4.Such induction is inversely correlated with downregulation of their cognate DAMs.We also show that the six DAMs were hypermethylated,associating with the production of 24-nt sRNAs.Hence,the chilling-responsive dynamic of the different epigenetic elements and their interactions likely define distinct expression abundance and downregulation pattern of each DAM.We further show that the expression of the five DAMs remains steadily unchanged or continuously downregulated at the ensuing warm temperature after chilling,and this state of regulation correlates with robust increase of sRNA expression,H3K27me3 and CHH methylation,which is particularly pronounced in DAM4.Such robust increase of repressive epigenetic marks may irreversibly reinforce the chillingimposed repression of DAMs to ensure flower-developmental programming free from any residual DAM inhibition.Taken together,we reveal novel information about genetic and epigenetic regulation of the DAM cluster in peach,which will be of fundamental significance in understanding of the regulatory mechanisms underlying chilling requirement and dormancy release,and of practical application for improvement of plasticity of flower time and bud break in fruit trees to adapt changing climates. | Hong Zhu Pao-Yang Chen Silin Zhong Chris Dardick Ann Callahan Yong-Qiang An Steve van Knocker Yingzhen Yang Gan-Yuan Zhong Albert Abbott Zongrang Liu | 2020 | Horticulture Research2020，7，1： | 9 |
| 4 | Anti-HIV Ⅰ/Ⅱ Activity and Molecular Cloning of a Novel Mannose/Sialic Acidbinding Lectin from Rhizome of Polygonatum cyrtonema Hua显示文摘 | Jie AN Jin-Zhi LIU Chuan-Fang WU Jian LI Lei DAI Els Van DAMME Jan BALZARINI Erik De CLERCQ Fang CHEN Jin-Ku BAO | 2006 | Acta Biochimica et Biophysica Sinica2006，38，2： | 8 |
| 5 | An INDEHISCENT-Controlled Auxin Response Specifies the Separation Layer in Early Arabidopsis Fruit显示文摘种子疏开是在植物种的生命周期的一重要时刻。在 Arabidopsis thaliana,它依赖于抄写因素果皮不裂(IND ) 调停了在裂开地区的分离层的说明在在阀门(心皮墙) 和发展中的水果的中央洋梅之间的边缘发现了。IND 由在水果开发的迟了的阶段导致本地植物生长素最小指定分离层,这被建议。这里,我们证明 ind 异种和野类型的水果之间的词法差别已经在水果开发的早阶段产生,与在阀门边缘的强壮的 IND 表示与一致。我们证明减少 IND 的 PIN-FORMED3 (PIN3 ) 植物生长素流出搬运人丰富在早水果开发期间在阀门边缘导致增加的植物生长素回答,并且形成裂开地区的伴随物房间部门缺乏 ind 变异的水果。而且, AGC kinases PINOID (PID ) 的 IND 倡导者驱动的宫外的表示和 WAG2 由通过增加的 PIN3 丰富在水果开发的阶段 14-16 从阀门边缘驱逐植物生长素导致了 indehiscence。我们的结果证明除它在 Arabidopsis 水果开发的迟了的阶段的角色以外, IND 在早阶段工作便于形成裂开地区的被触发植物生长素的房间部门。 | Kasper van Gelderen Martin van Rongen An'an Liu Anne Otten Remko Offringa | 2016 | Molecular Plant2016，9，6： | 3 |
| 6 | Carcinoma-associated fibroblasts provide operational flexibility in metastasis显示文摘 | Olivier De Wever Mieke Van Bockstal Marc Mareel An Hendrix Marc Bracke | 2014 | Seminars in Cancer Biology2014，，： | 2 |
| 7 | Better to be alone than in bad company:The antagonistic effect of cisplatin and crizotinib combination therapy in non-small cell lung cancer显示文摘AIM To investigate the potential benefit of combining the cMET inhibitor crizotinib and cisplatin we performed in vitro combination studies.METHODS We tested three different treatment schemes in four non-small cell lung cancer(NSCLC) cell lines with a different cMET/epidermal growth factor receptor genetic background by means of the sulforhodamine B assay and performed analysis with Calcusyn.RESULTS All treatment schemes showed an antagonistic effect in all cell lines,independent of the cMET status.Despite their different genetic backgrounds,all cell lines(EBC-1,HCC827,H1975 and LUDLU-1) showed antagonistic combination indexes ranging from 1.3-2.7.These results were independent of the treatment schedule.CONCLUSION These results discourage further efforts to combine cMET inhibition with cisplatin chemotherapy in NSCLC. | Nele Van Der Steen Christophe Deben Vanessa Deschoolmeester An Wouters Filip Lardon Christian Rolfo Paul Germonpré Elisa Giovannetti Godefridus J Peters Patrick Pauwels | 2016 | World Journal of Clinical Oncology2016，7，6： | 2 |
| 8 | Prostatic intraepithelial neoplasia and putative precursor lesions of prostate cancer:a clinical perspective显示文摘 | Vis AN Van Der Kwast TH | 2001 | BJU Int2001，88，2： | 1 |
| 9 | Postoperative radiotherapy for stage 1 endometrial carcinoma: long-term outcome of the randomized PORTEC trial with central pathology revlew显示文摘 | Scholten AN van Putten WL Beerman H | 2005 | Int J Radiat Oncol Biol Phys2005，63，3： | 1 |
| 10 | Signal regulatory proteins in the immune system显示文摘 | van Beek EM Cochrane F Barclay AN | 2005 | J Immu- nol2005，175，12： | 1 |
| 11 | Soft tissue carcinoma in the retropertoneum: an often neglected diagnosis显示文摘 | Van Geel AN Van Coevorden F | 2001 | Eur J Surg Oncol2001，27，1： | 1 |
| 12 | Potency of HIV-1 envelope glycoprotein gp120 antibodies to inhibit the interaction of DC-SIGN with HIV-1 gp120显示文摘 | Lekkerkerker AN Ludwig IS van Vliet SJ | 2004 | Virology2004，329，2： | 1 |
| 13 | 显示文摘 | Cheng A van Hoek AN Yenger M | 1997 | Nature1997，387，： | 1 |
| 14 | Direct measure- ment of spontaneous strategy selection in a virtual Morris water maze shows females choose an allocentric strategy at least as often as males do显示文摘 | Van Gerven DJ Schneider AN Wuitchik DM | 2012 | Behav Neurosci2012，126，： | 1 |
| 15 | Extraction of high-quality genomic DNA from latex-containing plants 显示文摘 | Michiels An Van den Ende Wim Tucker Mark | 2003 | Analytical Biochemistry2003，315，1： | 1 |
| 16 | The cutaneous Innervation of the femal breast and nipple aveola complex: implications for surgery显示文摘 | Jaspars JJ Posma AN Van Immerseel AA | 1997 | Br J Plast Surg1997，50，： | 1 |
| 17 | Vascular endothelial growth factors and angiogenesis in eye disease 显示文摘 | Witmer AN Vrensen GFJM van Niirden CJF | 2003 | Pro Ret Eye Res2003，22，： | 1 |
| 18 | The nicotinamide phosphoribosyhransferase: a molecular link between metabolism, inflammation, and cancer 显示文摘 | Galli M Van Gool F Rongvaux A An&is F Leo O | 2010 | Cancer Res2010，70，： | 1 |
| 19 | Midterm clinical and magnetic resonance imaging follow-up of large and giant carotid artery aneurysms after therapeutic carotid artery occlusion显示文摘 | De Gast AN Sprengers ME van Rooij WJ | 2007 | Neurosurgery2007，60，6： | 1 |
| 20 | Prognosis of primary mucosal penile melanoma:a series of 19 Dutch patients and 47 patients from the literature显示文摘 | van Geel AN den Bakker MA Kirkels W | | 0，，： | 1 |
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