ZmMs30 Encoding a Novel GDSL Lipase Is Essential for Male Fertility and Valuable for Hybrid Breeding in Maize

查看全文 作　　者：Xueli [1,2]An;Zhenying [1,2]Dong;Youhui [1,2]Tian;Ke [1,2]Xie;Suowei [1,2]Wu;Taotao [1]Zhu;Danfeng [2]Zhang;Van [2]Zhou;Canfang [1,2]Niu;Biao [1,2]Ma;Quancan [1,2]Hou;Jianxi [1]Bao;Simiao [1]Zhang;Ziwen [1,2]Li;Yanbo [1]Wang;Tingwei [1]Yan;Xiaojing [1]Sun;Yuwen [1,2]Zhang;Jinping [2]Li;Xiangyuan [1,2]Wan

机构地区：[1]Biology and Agriculture Research Center,University of Science and Technology Beijing,Beijing 100024,China;[2]Beijing Engineering Laboratory of Main Crop Bio-Tech Breeding,Beijing International Science and Technology Cooperation Base of Bio-Tech Breeding,Beijing Solidwill Sci-Tech Co.Ltd.,Beijing 100192,China

出　　处：《Molecular Plant》索引2019年第12卷第3期,共17页

基　　金：the National Key Research and Development Program of China (2018YFD0100806,2017YFD0102001,2017YFD0101201);the National Transgenic Major Program of China (2018ZX0801006B,2018ZX0800922B);the National Natural Science Foundation of China (31771875,31871702);the Fundamental Research Funds for the Central Universities of China (06500060;FRF-BR-17- 009A,-010A;and -011 A);the 'Ten Thousand Plann-National High Level Talents Special Support Plan (for X.W.);the National Key Technology R&D Program of China (2014BAD01B02);and the Beijing Science & Technology Plan Program (Z161100000916013). 摘　　要：Genic male sterility (GMS) is very useful for hybrid vigor utilization and hybrid seed production. Although a large number of GMS genes have been identified in plants, little is known about the roles of GDSL lipase members in anther and pollen development. Here, we report a maize GMS gene, ZmMs30, which encodes a novel type of GDSL lipase with diverged catalytic residues. Enzyme kinetics and activity assays show that ZmMs30 has lipase activity and prefers to substrates with a short carbon chain. ZmMs30 is specifically expressed in maize anthers during stages 7-9. Loss of ZmMs30 function resulted in defective anther cuticle, irregular foot layer of pollen exine, and complete male sterility. Cytological and lipidomics analyses demonstrate that ZmMs30 is crucial for the aliphatic metabolic pathway required for pollen exine formation and anther cuticle development. Furthermore, we found that male sterility caused by loss of ZmMs30 function was stable in various inbred lines with different genetic background, and that it didn't show any negative effect on maize heterosis and production, suggesting that ZmMs30 is valuable for crossbreeding and hybrid seed production. We then developed a new multi-control sterility system using ZmMs30 and its mutant line, and demonstrated it is feasible for generating desirable GMS lines and valu. able for hybrid maize seed production. Taken together, our study sheds new light on the mechanisms of anther and pollen development, and provides a valuable male-sterility system for hybrid breeding maize. 关键词：ZmMs30f GDSL LIPASE multi-control STERILITY (MCS) system hybrid SEED production MAIZE

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ZmMs30 Encoding a Novel GDSL Lipase Is Essential for Male Fertility and Valuable for Hybrid Breeding in Maize

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